LOCUS       RC214301                7833 bp    DNA     circular  SYN 
DEFINITION  EPHB1 (Myc-DDK-tagged)-Human EPH receptor B1 (EPHB1)
ACCESSION   NM_004441
VERSION     NM_004441.3
SOURCE      synthetic construct 
FEATURES    Location/Qualifiers 
     misc_feature    201..924 
                     /label="CMV promoter"  
     misc_feature    839..856 
                     /label="Restriction Enzyme"  
     misc_feature    953..971 
                     /label="T7 promoter"  
     misc_feature    3990..4081 
                     /label="Myc-DDK"  
     misc_feature    4111..4696 
                     /label="PolyA signal"  
     misc_feature    4168..4187 
                     /label="Restriction Enzyme"  
     misc_feature    4972..5644 
                     /label="ColE1"  
     misc_feature    6074..6868 
                     /label="Kan/Neo"  
     misc_feature    6903..7205 
                     /label="SV40 ori"  
     misc_feature    7291..7768 
                     /label="f1 ori"  
     misc_feature    1020..1028 
                     /label="Restriction Enzyme"  
     misc_feature    3972..3978 
                     /label="Restriction Enzyme"  
     misc_feature    1020..3972 
                     /label="EPHB1"  
ORIGIN                      
        1 AACAAAATAT TAACGCTTAC AATTTCCATT CGCCATTCAG GCTGCGCAAC TGTTGGGAAG 
       61 GGCGATCGGT GCGGGCCTCT TCGCTATTAC GCCAGCTGGC GAAAGGGGGA TGTGCTGCAA 
      121 GGCGATTAAG TTGGGTAACG CCAGGGTTTT CCCAGTCACG ACGTTGTAAA ACGACGGCCA 
      181 GTGCCAAGCT GATCTATACA TTGAATCAAT ATTGGCAATT AGCCATATTA GTCATTGGTT 
      241 ATATAGCATA AATCAATATT GGCTATTGGC CATTGCATAC GTTGTATCTA TATCATAATA 
      301 TGTACATTTA TATTGGCTCA TGTCCAATAT GACCGCCATG TTGACATTGA TTATTGACTA 
      361 GTTATTAATA GTAATCAATT ACGGGGTCAT TAGTTCATAG CCCATATATG GAGTTCCGCG 
      421 TTACATAACT TACGGTAAAT GGCCCGCCTG GCTGACCGCC CAACGACCCC CGCCCATTGA 
      481 CGTCAATAAT GACGTATGTT CCCATAGTAA CGCCAATAGG GACTTTCCAT TGACGTCAAT 
      541 GGGTGGAGTA TTTACGGTAA ACTGCCCACT TGGCAGTACA TCAAGTGTAT CATATGCCAA 
      601 GTCCGCCCCC TATTGACGTC AATGACGGTA AATGGCCCGC CTGGCATTAT GCCCAGTACA 
      661 TGACCTTACG GGACTTTCCT ACTTGGCAGT ACATCTACGT ATTAGTCATC GCTATTACCA 
      721 TGGTGATGCG GTTTTGGCAG TACACCAATG GGCGTGGATA GCGGTTTGAC TCACGGGGAT 
      781 TTCCAAGTCT CCACCCCATT GACGTCAATG GGAGTTTGTT TTGGCACCAA AATCAACGGG 
      841 ACTTTCCAAA ATGTCGTAAT AACCCCGCCC CGTTGACGCA AATGGGCGGT AGGCGTGTAC 
      901 GGTGGGAGGT CTATATAAGC AGAGCTCGTT TAGTGAACCG TCAGAATTTT GTAATACGAC 
      961 TCACTATAGG GCGGCCGGGA ATTCGTCGAC TGGATCCGGT ACCGAGGAGA TCTGCCGCCG 
     1021 CGATCGCCAT GGCCCTGGAT TATCTACTAC TGCTCCTCCT GGCATCCGCA GTGGCTGCGA 
     1081 TGGAAGAAAC GTTAATGGAC ACCAGAACGG CTACTGCAGA GCTGGGCTGG ACGGCCAATC 
     1141 CTGCGTCCGG GTGGGAAGAA GTCAGTGGCT ACGATGAAAA CCTGAACACC ATCCGCACCT 
     1201 ACCAGGTGTG CAATGTCTTC GAGCCCAACC AGAACAATTG GCTGCTCACC ACCTTCATCA 
     1261 ACCGGCGGGG GGCCCATCGC ATCTACACAG AGATGCGCTT CACTGTGAGA GACTGCAGCA 
     1321 GCCTCCCTAA TGTCCCAGGA TCCTGCAAGG AGACCTTCAA CTTGTATTAC TATGAGACTG 
     1381 ACTCTGTCAT TGCCACCAAG AAGTCAGCCT TCTGGTCTGA GGCCCCCTAC CTCAAAGTAG 
     1441 ACACCATTGC TGCAGATGAG AGCTTCTCCC AGGTGGACTT TGGGGGAAGG CTGATGAAGG 
     1501 TAAACACAGA AGTCAGGAGC TTTGGGCCTC TTACTCGGAA TGGTTTTTAC CTCGCTTTTC 
     1561 AGGATTATGG AGCCTGTATG TCTCTTCTTT CTGTCCGTGT CTTCTTCAAA AAGTGTCCCA 
     1621 GCATTGTGCA AAATTTTGCA GTGTTTCCAG AGACTATGAC AGGGGCAGAG AGCACATCTC 
     1681 TGGTGATTGC TCGGGGCACA TGCATCCCCA ACGCAGAGGA AGTGGACGTG CCCATCAAAC 
     1741 TCTACTGCAA CGGGGATGGG GAATGGATGG TGCCTATTGG GCGATGCACC TGCAAGCCTG 
     1801 GCTATGAGCC TGAGAACAGC GTGGCATGCA AGGCTTGCCC TGCAGGGACA TTCAAGGCCA 
     1861 GCCAGGAAGC TGAAGGCTGC TCCCACTGCC CCTCCAACAG CCGCTCCCCT GCAGAGGCGT 
     1921 CTCCCATCTG CACCTGTCGG ACCGGTTATT ACCGAGCGGA CTTTGACCCT CCAGAAGTGG 
     1981 CATGCACTAG CGTCCCATCA GGTCCCCGCA ATGTTATCTC CATCGTCAAT GAGACGTCCA 
     2041 TCATTCTGGA GTGGCACCCT CCAAGGGAGA CAGGTGGGCG GGATGATGTG ACCTACAACA 
     2101 TCATCTGCAA AAAGTGCCGG GCAGACCGCC GGAGCTGCTC CCGCTGTGAC GACAATGTGG 
     2161 AGTTTGTGCC CAGGCAGCTG GGCCTGACGG AGTGCCGCGT CTCCATCAGC AGCCTGTGGG 
     2221 CCCACACCCC CTACACCTTT GACATCCAGG CCATCAATGG AGTCTCCAGC AAGAGTCCCT 
     2281 TCCCCCCACA GCACGTCTCT GTCAACATCA CCACAAACCA AGCCGCCCCC TCCACCGTTC 
     2341 CCATCATGCA CCAAGTCAGT GCCACTATGA GGAGCATCAC CTTGTCATGG CCACAGCCGG 
     2401 AGCAGCCCAA TGGCATCATC CTGGACTATG AGATCCGGTA CTATGAGAAG GAACACAATG 
     2461 AGTTCAACTC CTCCATGGCC AGGAGTCAGA CCAACACAGC AAGGATTGAT GGGCTGCGGC 
     2521 CTGGCATGGT ATATGTGGTA CAGGTGCGTG CCCGCACTGT TGCTGGCTAC GGCAAGTTCA 
     2581 GTGGCAAGAT GTGCTTCCAG ACTCTGACTG ACGATGATTA CAAGTCAGAG CTGAGGGAGC 
     2641 AGCTGCCCCT GATTGCTGGC TCGGCAGCGG CCGGGGTCGT GTTCGTTGTG TCCTTGGTGG 
     2701 CCATCTCTAT CGTCTGTAGC AGGAAACGGG CTTATAGCAA AGAGGCTGTG TACAGCGATA 
     2761 AGCTCCAGCA TTACAGCACA GGCCGAGGCT CCCCAGGGAT GAAGATCTAC ATTGACCCCT 
     2821 TCACTTATGA GGATCCCAAC GAAGCTGTCC GGGAGTTTGC CAAGGAGATT GATGTATCTT 
     2881 TTGTGAAAAT TGAAGAGGTC ATCGGAGCAG GGGAGTTTGG AGAAGTGTAC AAGGGGCGTT 
     2941 TGAAACTGCC AGGCAAGAGG GAAATCTACG TGGCCATCAA GACCCTGAAG GCAGGGTACT 
     3001 CGGAGAAGCA GCGTCGGGAC TTTCTGAGTG AGGCGAGCAT CATGGGCCAG TTCGACCATC 
     3061 CTAACATCAT TCGCCTGGAG GGTGTGGTCA CCAAGAGTCG GCCTGTCATG ATCATCACAG 
     3121 AGTTCATGGA GAATGGTGCA TTGGATTCTT TCCTCAGGCA AAATGACGGG CAGTTCACCG 
     3181 TGATCCAGCT TGTGGGTATG CTCAGGGGCA TCGCTGCTGG CATGAAGTAC CTGGCTGAGA 
     3241 TGAATTATGT GCATCGGGAC CTGGCTGCTA GGAACATTCT GCTCAACAGT AACCTGGTGT 
     3301 GCAAGGTGTC CGACTTTGGC CTCTCCCGCT ACCTCCAGGA TGACACCTCA GATCCCACCT 
     3361 ACACCAGCTC CTTGGGAGGG AAGATCCCTG TGAGATGGAC AGCTCCAGAG GCCATCGCCT 
     3421 ACCGCAAGTT CACTTCAGCC AGCGACGTTT GGAGCTATGG GATCGTCATG TGGGAAGTCA 
     3481 TGTCATTTGG AGAGAGACCC TATTGGGATA TGTCCAACCA AGATGTCATC AATGCCATCG 
     3541 AGCAGGACTA CCGGCTGCCC CCACCCATGG ACTGTCCAGC TGCTCTACAC CAGCTCATGC 
     3601 TGGACTGTTG GCAGAAGGAC CGGAACAGCC GGCCCCGGTT TGCGGAGATT GTCAACACCC 
     3661 TAGATAAGAT GATCCGGAAC CCGGCAAGTC TCAAGACTGT GGCAACCATC ACCGCCGTGC 
     3721 CTTCCCAGCC CCTGCTCGAC CGCTCCATCC CAGACTTCAC GGCCTTTACC ACCGTGGATG 
     3781 ACTGGCTCAG CGCCATCAAA ATGGTCCAGT ACAGGGACAG CTTCCTCACT GCTGGCTTCA 
     3841 CCTCCCTCCA GCTGGTCACC CAGATGACAT CAGAAGACCT CCTGAGAATA GGCATCACCT 
     3901 TGGCAGGCCA TCAGAAGAAG ATCCTGAACA GCATTCATTC TATGAGGGTC CAGATAAGTC 
     3961 AGTCACCAAC GGCAATGGCA ACGCGTACGC GGCCGCTCGA GCAGAAACTC ATCTCAGAAG 
     4021 AGGATCTGGC AGCAAATGAT ATCCTGGATT ACAAGGATGA CGACGATAAG GTTTAAACGG 
     4081 CCGGCCGCGG TCATAGCTGT TTCCTGAACA GATCCCGGGT GGCATCCCTG TGACCCCTCC 
     4141 CCAGTGCCTC TCCTGGCCCT GGAAGTTGCC ACTCCAGTGC CCACCAGCCT TGTCCTAATA 
     4201 AAATTAAGTT GCATCATTTT GTCTGACTAG GTGTCCTTCT ATAATATTAT GGGGTGGAGG 
     4261 GGGGTGGTAT GGAGCAAGGG GCAAGTTGGG AAGACAACCT GTAGGGCCTG CGGGGTCTAT 
     4321 TGGGAACCAA GCTGGAGTGC AGTGGCACAA TCTTGGCTCA CTGCAATCTC CGCCTCCTGG 
     4381 GTTCAAGCGA TTCTCCTGCC TCAGCCTCCC GAGTTGTTGG GATTCCAGGC ATGCATGACC 
     4441 AGGCTCAGCT AATTTTTGTT TTTTTGGTAG AGACGGGGTT TCACCATATT GGCCAGGCTG 
     4501 GTCTCCAACT CCTAATCTCA GGTGATCTAC CCACCTTGGC CTCCCAAATT GCTGGGATTA 
     4561 CAGGCGTGAA CCACTGCTCC CTTCCCTGTC CTTCTGATTT TAAAATAACT ATACCAGCAG 
     4621 GAGGACGTCC AGACACAGCA TAGGCTACCT GGCCATGCCC AACCGGTGGG ACATTTGAGT 
     4681 TGCTTGCTTG GCACTGTCCT CTCATGCGTT GGGTCCACTC AGTAGATGCC TGTTGAATTG 
     4741 GGTACGCGGC CAGCGGCGAG CGGTATCAGC TCACTCAAAG GCGGTAATAC GGTTATCCAC 
     4801 AGAATCAGGG GATAACGCAG GAAAGAACAT GTGAGCAAAA GGCCAGCAAA AGGCCAGGAA 
     4861 CCGTAAAAAG GCCGCGTTGC TGGCGTTTTT CCATAGGCTC CGCCCCCCTG ACGAGCATCA 
     4921 CAAAAATCGA CGCTCAAGTC AGAGGTGGCG AAACCCGACA GGACTATAAA GATACCAGGC 
     4981 GTTTCCCCCT GGAAGCTCCC TCGTGCGCTC TCCTGTTCCG ACCCTGCCGC TTACCGGATA 
     5041 CCTGTCCGCC TTTCTCCCTT CGGGAAGCGT GGCGCTTTCT CATAGCTCAC GCTGTAGGTA 
     5101 TCTCAGTTCG GTGTAGGTCG TTCGCTCCAA GCTGGGCTGT GTGCACGAAC CCCCCGTTCA 
     5161 GCCCGACCGC TGCGCCTTAT CCGGTAACTA TCGTCTTGAG TCCAACCCGG TAAGACACGA 
     5221 CTTATCGCCA CTGGCAGCAG CCACTGGTAA CAGGATTAGC AGAGCGAGGT ATGTAGGCGG 
     5281 TGCTACAGAG TTCTTGAAGT GGTGGCCTAA CTACGGCTAC ACTAGAAGAA CAGTATTTGG 
     5341 TATCTGCGCT CTGCTGAAGC CAGTTACCTT CGGAAAAAGA GTTGGTAGCT CTTGATCCGG 
     5401 CAAACAAACC ACCGCTGGTA GCGGTGGTTT TTTTGTTTGC AAGCAGCAGA TTACGCGCAG 
     5461 AAAAAAAGGA TCTCAAGAAG ATCCTTTGAT CTTTTCTACG GGGTCTGACG CTCAGTGGAA 
     5521 CGAAAACTCA CGTTAAGGGA TTTTGGTCAT GAGATTATCA AAAAGGATCT TCACCTAGAT 
     5581 CCTTTTAAAT TAAAAATGAA GTTTTAAATC AATCTAAAGT ATATATGAGT AACCTGAGGC 
     5641 TATGGCAGGG CCTGCCGCCC CGACGTTGGC TGCGAGCCCT GGGCCTTCAC CCGAACTTGG 
     5701 GGGGTGGGGT GGGGAAAAGG AAGAAACGCG GGCGTATTGG CCCCAATGGG GTCTCGGTGG 
     5761 GGTATCGACA GAGTGCCAGC CCTGGGACCG AACCCCGCGT TTATGAACAA ACGACCCAAC 
     5821 ACCGTGCGTT TTATTCTGTC TTTTTATTGC CGTCATAGCG CGGGTTCCTT CCGGTATTGT 
     5881 CTCCTTCCGT GTTTCAGTTA GCCTCCCCCT AGGGTGGGCG AAGAACTCCA GCATGAGATC 
     5941 CCCGCGCTGG AGGATCATCC AGCCGGCGTC CCGGAAAACG ATTCCGAAGC CCAACCTTTC 
     6001 ATAGAAGGCG GCGGTGGAAT CGAAATCTCG TGATGGCAGG TTGGGCGTCG CTTGGTCGGT 
     6061 CATTTCGAAC CCCAGAGTCC CGCTCAGAAG AACTCGTCAA GAAGGCGATA GAAGGCGATG 
     6121 CGCTGCGAAT CGGGAGCGGC GATACCGTAA AGCACGAGGA AGCGGTCAGC CCATTCGCCG 
     6181 CCAAGCTCTT CAGCAATATC ACGGGTAGCC AACGCTATGT CCTGATAGCG ATCCGCCACA 
     6241 CCCAGCCGGC CACAGTCGAT GAATCCAGAA AAGCGGCCAT TTTCCACCAT GATATTCGGC 
     6301 AAGCAGGCAT CGCCATGGGT CACGACGAGA TCCTCGCCGT CGGGCATGCT CGCCTTGAGC 
     6361 CTGGCGAACA GTTCGGCTGG CGCGAGCCCC TGATGCTCTT CGTCCAGATC ATCCTGATCG 
     6421 ACAAGACCGG CTTCCATCCG AGTACGTGCT CGCTCGATGC GATGTTTCGC TTGGTGGTCG 
     6481 AATGGGCAGG TAGCCGGATC AAGCGTATGC AGCCGCCGCA TTGCATCAGC CATGATGGAT 
     6541 ACTTTCTCGG CAGGAGCAAG GTGAGATGAC AGGAGATCCT GCCCCGGCAC TTCGCCCAAT 
     6601 AGCAGCCAGT CCCTTCCCGC TTCAGTGACA ACGTCGAGCA CAGCTGCGCA AGGAACGCCC 
     6661 GTCGTGGCCA GCCACGATAG CCGCGCTGCC TCGTCTTGCA GTTCATTCAG GGCACCGGAC 
     6721 AGGTCGGTCT TGACAAAAAG AACCGGGCGC CCCTGCGCTG ACAGCCGGAA CACGGCGGCA 
     6781 TCAGAGCAGC CGATTGTCTG TTGTGCCCAG TCATAGCCGA ATAGCCTCTC CACCCAAGCG 
     6841 GCCGGAGAAC CTGCGTGCAA TCCATCTTGT TCAATCATGC GAAACGATCC TCATCCTGTC 
     6901 TCTTGATCGA TCTTTGCAAA AGCCTAGGCC TCCAAAAAAG CCTCCTCACT ACTTCTGGAA 
     6961 TAGCTCAGAG GCCGAGGCGG CCTCGGCCTC TGCATAAATA AAAAAAATTA GTCAGCCATG 
     7021 GGGCGGAGAA TGGGCGGAAC TGGGCGGAGT TAGGGGCGGG ATGGGCGGAG TTAGGGGCGG 
     7081 GACTATGGTT GCTGACTAAT TGAGATGCAT GCTTTGCATA CTTCTGCCTG CTGGGGAGCC 
     7141 TGGGGACTTT CCACACCTGG TTGCTGACTA ATTGAGATGC ATGCTTTGCA TACTTCTGCC 
     7201 TGCTGGGGAG CCTGGGGACT TTCCACACCC TAACTGACAC ACATTCCACA GCTGGTTCTT 
     7261 TCCGCCTCAG GACTCTTCCT TTTTCAATAT TATTGAAGCA TTTATCAGGG TTATTGTCTC 
     7321 ATGAGCGGAT ACATATTTGA ATGTATTTAG AAAAATAAAC AAATAGGGGT TCCGCGCACA 
     7381 TTTCCCCGAA AAGTGCCACC TGACGCGCCC TGTAGCGGCG CATTAAGCGC GGCGGGTGTG 
     7441 GTGGTTACGC GCAGCGTGAC CGCTACACTT GCCAGCGCCC TAGCGCCCGC TCCTTTCGCT 
     7501 TTCTTCCCTT CCTTTCTCGC CACGTTCGCC GGCTTTCCCC GTCAAGCTCT AAATCGGGGG 
     7561 CTCCCTTTAG GGTTCCGATT TAGTGCTTTA CGGCACCTCG ACCCCAAAAA ACTTGATTAG 
     7621 GGTGATGGTT CACGTAGTGG GCCATCGCCC TGATAGACGG TTTTTCGCCC TTTGACGTTG 
     7681 GAGTCCACGT TCTTTAATAG TGGACTCTTG TTCCAAACTG GAACAACACT CAACCCTATC 
     7741 TCGGTCTATT CTTTTGATTT ATAAGGGATT TTGCCGATTT CGGCCTATTG GTTAAAAAAT 
     7801 GAGCTGATTT AACAAAAATT TAACGCGAAT TTT
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