LOCUS       RC214297                8586 bp    DNA     circular  SYN 
DEFINITION  TAOK2 (Myc-DDK-tagged)-Human TAO kinase 2 (TAOK2), transcript variant 2
ACCESSION   NM_016151
VERSION     NM_016151.2
SOURCE      synthetic construct 
FEATURES    Location/Qualifiers 
     misc_feature    201..924 
                     /label="CMV promoter"  
     misc_feature    839..856 
                     /label="Restriction Enzyme"  
     misc_feature    953..971 
                     /label="T7 promoter"  
     misc_feature    4743..4834 
                     /label="Myc-DDK"  
     misc_feature    4864..5449 
                     /label="PolyA signal"  
     misc_feature    4921..4940 
                     /label="Restriction Enzyme"  
     misc_feature    5725..6397 
                     /label="ColE1"  
     misc_feature    6827..7621 
                     /label="Kan/Neo"  
     misc_feature    7656..7958 
                     /label="SV40 ori"  
     misc_feature    8044..8521 
                     /label="f1 ori"  
     misc_feature    1020..1028 
                     /label="Restriction Enzyme"  
     misc_feature    4725..4731 
                     /label="Restriction Enzyme"  
     misc_feature    1020..4725 
                     /label="TAOK2"  
ORIGIN                      
        1 AACAAAATAT TAACGCTTAC AATTTCCATT CGCCATTCAG GCTGCGCAAC TGTTGGGAAG 
       61 GGCGATCGGT GCGGGCCTCT TCGCTATTAC GCCAGCTGGC GAAAGGGGGA TGTGCTGCAA 
      121 GGCGATTAAG TTGGGTAACG CCAGGGTTTT CCCAGTCACG ACGTTGTAAA ACGACGGCCA 
      181 GTGCCAAGCT GATCTATACA TTGAATCAAT ATTGGCAATT AGCCATATTA GTCATTGGTT 
      241 ATATAGCATA AATCAATATT GGCTATTGGC CATTGCATAC GTTGTATCTA TATCATAATA 
      301 TGTACATTTA TATTGGCTCA TGTCCAATAT GACCGCCATG TTGACATTGA TTATTGACTA 
      361 GTTATTAATA GTAATCAATT ACGGGGTCAT TAGTTCATAG CCCATATATG GAGTTCCGCG 
      421 TTACATAACT TACGGTAAAT GGCCCGCCTG GCTGACCGCC CAACGACCCC CGCCCATTGA 
      481 CGTCAATAAT GACGTATGTT CCCATAGTAA CGCCAATAGG GACTTTCCAT TGACGTCAAT 
      541 GGGTGGAGTA TTTACGGTAA ACTGCCCACT TGGCAGTACA TCAAGTGTAT CATATGCCAA 
      601 GTCCGCCCCC TATTGACGTC AATGACGGTA AATGGCCCGC CTGGCATTAT GCCCAGTACA 
      661 TGACCTTACG GGACTTTCCT ACTTGGCAGT ACATCTACGT ATTAGTCATC GCTATTACCA 
      721 TGGTGATGCG GTTTTGGCAG TACACCAATG GGCGTGGATA GCGGTTTGAC TCACGGGGAT 
      781 TTCCAAGTCT CCACCCCATT GACGTCAATG GGAGTTTGTT TTGGCACCAA AATCAACGGG 
      841 ACTTTCCAAA ATGTCGTAAT AACCCCGCCC CGTTGACGCA AATGGGCGGT AGGCGTGTAC 
      901 GGTGGGAGGT CTATATAAGC AGAGCTCGTT TAGTGAACCG TCAGAATTTT GTAATACGAC 
      961 TCACTATAGG GCGGCCGGGA ATTCGTCGAC TGGATCCGGT ACCGAGGAGA TCTGCCGCCG 
     1021 CGATCGCCAT GCCAGCTGGG GGCCGGGCCG GGAGCCTGAA GGACCCAGAT GTGGCTGAGC 
     1081 TCTTCTTCAA GGATGACCCA GAAAAGCTCT TCTCTGACCT CCGGGAAATT GGCCATGGCA 
     1141 GCTTTGGAGC CGTATACTTT GCCCGGGATG TCCGGAATAG TGAGGTGGTG GCCATCAAGA 
     1201 AGATGTCCTA CAGTGGGAAG CAGTCCAATG AGAAATGGCA AGACATCATC AAGGAGGTGC 
     1261 GGTTCTTACA GAAGCTCCGG CATCCCAACA CCATTCAGTA CCGGGGCTGT TACCTGAGGG 
     1321 AGCACACGGC TTGGCTGGTA ATGGAGTATT GCCTGGGCTC AGCTTCTGAC CTTCTAGAAG 
     1381 TGCACAAGAA ACCCCTTCAG GAGGTAGAGA TCGCAGCTGT GACCCACGGG GCGCTTCAGG 
     1441 GCCTGGCATA TCTGCACTCC CACAACATGA TCCATAGGGA TGTGAAGGCT GGAAACATCC 
     1501 TGCTGTCAGA GCCAGGGTTA GTGAAGCTAG GGGACTTTGG TTCTGCGTCC ATCATGGCAC 
     1561 CTGCCAACTC CTTCGTGGGC ACCCCATACT GGATGGCACC CGAGGTGATC CTGGCCATGG 
     1621 ATGAGGGGCA GTACGATGGC AAAGTGGACG TCTGGTCCTT GGGGATAACC TGCATCGAGC 
     1681 TGGCTGAACG GAAACCACCG CTCTTTAACA TGAATGCGAT GAGTGCCTTA TACCACATTG 
     1741 CACAGAACGA ATCCCCCGTG CTCCAGTCAG GACACTGGTC TGAGTACTTC CGGAATTTTG 
     1801 TCGACTCCTG TCTTCAGAAA ATCCCTCAAG ACAGACCAAC CTCAGAGGTT CTCCTGAAGC 
     1861 ACCGCTTTGT GCTCCGGGAG CGGCCACCCA CAGTCATCAT GGACCTGATC CAGAGGACCA 
     1921 AGGATGCCGT GCGGGAGCTG GACAACCTGC AGTACCGCAA GATGAAGAAG ATCCTGTTCC 
     1981 AAGAGGCACC CAACGGCCCT GGTGCCGAGG CCCCAGAGGA GGAAGAGGAG GCCGAGCCCT 
     2041 ACATGCACCG GGCCGGGACT CTGACCAGCC TCGAGAGTAG CCACTCAGTG CCCAGCATGT 
     2101 CCATCAGCGC CTCCAGCCAG AGCAGCTCCG TCAACAGCCT AGCAGATGCC TCAGACAACG 
     2161 AGGAAGAGGA GGAGGAGGAG GAGGAAGAGG AGGAGGAGGA AGAAGGCCCT GAAGCCCGGG 
     2221 AGATGGCCAT GATGCAGGAG GGGGAGCACA CAGTCACCTC TCACAGCTCC ATTATCCACC 
     2281 GGCTGCCGGG CTCTGACAAC CTATATGATG ACCCCTACCA GCCAGAGATA ACCCCCAGCC 
     2341 CTCTCCAGCC GCCTGCAGCC CCAGCTCCCA CTTCCACCAC CTCTTCCGCC CGCCGCCGGG 
     2401 CCTACTGCCG TAACCGAGAC CACTTTGCCA CCATCCGAAC CGCCTCCCTG GTCAGCCGTC 
     2461 AGATCCAGGA GCATGAGCAG GACTCTGCGC TGCGGGAGCA GCTGAGCGGC TATAAGCGGA 
     2521 TGCGACGACA GCACCAGAAG CAGCTGCTGG CCCTGGAGTC ACGGCTGAGG GGTGAACGGG 
     2581 AGGAGCACAG TGCACGGCTG CAGCGGGAGC TTGAGGCGCA GCGGGCTGGC TTTGGGGCAG 
     2641 AGGCAGAAAA GCTGGCCCGG CGGCACCAGG CCATAGGTGA GAAGGAGGCA CGAGCTGCCC 
     2701 AGGCCGAGGA GCGGAAGTTC CAGCAGCACA TCCTTGGGCA GCAGAAGAAG GAGCTGGCTG 
     2761 CCCTGCTGGA GGCACAGAAG CGGACCTACA AACTTCGCAA GGAACAGCTG AAGGAGGAGC 
     2821 TCCAGGAGAA CCCCAGCACT CCCAAGCGGG AGAAGGCCGA GTGGCTGCTG CGGCAGAAGG 
     2881 AGCAGCTCCA GCAGTGCCAG GCGGAGGAGG AAGCAGGGCT GCTGCGGCGG CAGCGCCAGT 
     2941 ACTTTGAGCT GCAGTGTCGC CAGTACAAGC GCAAGATGTT GCTGGCTCGG CACAGCCTGG 
     3001 ACCAGGACCT GCTGCGGGAG GACCTGAACA AGAAGCAGAC CCAGAAGGAC TTGGAGTGTG 
     3061 CACTGCTGCT TCGGCAGCAC GAGGCCACGC GGGAGCTGGA GCTGCGGCAG CTCCAGGCCG 
     3121 TGCAGCGCAC GCGGGCTGAG CTCACCCGCC TGCAGCACCA GACGGAGCTG GGCAACCAGC 
     3181 TGGAGTACAA CAAGCGGCGT GAGCAAGAGT TGCGGCAGAA GCATGCGGCC CAGGTTCGCC 
     3241 AGCAGCCCAA GAGCCTCAAA GTACGTGCAG GCCAGCGCCC CCCGGGCCTT CCACTCCCCA 
     3301 TTCCTGGGGC TCTGGGCCCA CCCAACACAG GCACCCCTAT AGAACAGCAG CCCTGCTCAC 
     3361 CTGGCCAGGA GGCAGTCCTG GACCAAAGAA TGCTTGGCGA GGAGGAGGAA GCAGTTGGAG 
     3421 AGAGAAGGAT TCTGGGAAAG GAAGGGGCCA CTTTGGAGCC CAAGCAGCAG AGGATTCTGG 
     3481 GGGAAGAATC AGGAGCCCCT AGTCCCAGTC CACAAAAACA TGGGAGCCTG GTTGATGAGG 
     3541 AAGTTTGGGG TCTGCCTGAG GAGATAGAGG AGCTTAGGGT GCCCTCCCTT GTACCCCAGG 
     3601 AGAGGAGCAT TGTTGGCCAG GAGGAGGCTG GGACATGGAG CTTGTGGGGG AAGGAGGATG 
     3661 AGAGTCTTCT GGATGAGGAG TTTGAGCTTG GCTGGGTCCA GGGCCCAGCA CTGACTCCCG 
     3721 TCCCTGAGGA GGAGGAAGAA GAGGAAGAGG GGGCTCCGAT TGGGACCCCT AGGGATCCTG 
     3781 GAGATGGTTG TCCTTCCCCC GACATCCCTC CTGAACCCCC TCCAACACAC CTGAGGCCCT 
     3841 GCCCTGCCAG CCAGCTCCCT GGACTCCTGT CCCATGGCCT CCTGGCCGGC CTCTCCTTTG 
     3901 CAGTGGGGTC CTCCTCTGGC CTCCTGCCCC TCCTGCTGCT GCTGCTGCTT CCATTGCTGG 
     3961 CAGCCCAGGG TGGGGGTGGC CTGCAGGCAG CGCTGCTGGC CCTTGAGGTG GGGCTGGTGG 
     4021 GTCTGGGGGC CTCCTACCTG CTCCTTTGTA CAGCCCTGCA CCTGCCCTCC AGTCTTTTCC 
     4081 TACTCCTGGC CCAGGGTACC GCACTGGGGG CCGTCCTGGG CCTGAGCTGG CGCCGAGGCC 
     4141 TCATGGGTGT TCCCCTGGGC CTTGGAGCTG CCTGGCTCTT AGCTTGGCCA GGCCTAGCTC 
     4201 TACCTCTGGT GGCTATGGCA GCGGGGGGCA GATGGGTGCG GCAGCAGGGC CCCCGGGTGC 
     4261 GCCGGGGCAT ATCTCGACTC TGGTTGCGGG TTCTGCTGCG CCTGTCACCC ATGGCCTTCC 
     4321 GGGCCCTGCA GGGCTGTGGG GCTGTGGGGG ACCGGGGTCT GTTTGCACTG TACCCCAAAA 
     4381 CCAACAAGGA TGGCTTCCGC AGCCGCCTGC CCGTCCCTGG GCCCCGGCGG CGTAATCCCC 
     4441 GCACCACCCA ACACCCATTA GCTCTGTTGG CAAGGGTCTG GGTCCTGTGC AAGGGCTGGA 
     4501 ACTGGCGTCT GGCACGGGCC AGCCAGGGTT TAGCATCCCA CTTGCCCCCG TGGGCCATCC 
     4561 ACACACTGGC CAGCTGGGGC CTGCTTCGGG GTGAACGGCC CACCCGAATC CCCCGGCTAC 
     4621 TACCACGCAG CCAGCGCCAG CTAGGGCCCC CTGCCTCCCG CCAGCCACTG CCAGGGACTC 
     4681 TAGCCGGGCG GAGGTCACGC ACCCGCCAGT CCCGGGCCCT GCCCCCCTGG AGGACGCGTA 
     4741 CGCGGCCGCT CGAGCAGAAA CTCATCTCAG AAGAGGATCT GGCAGCAAAT GATATCCTGG 
     4801 ATTACAAGGA TGACGACGAT AAGGTTTAAA CGGCCGGCCG CGGTCATAGC TGTTTCCTGA 
     4861 ACAGATCCCG GGTGGCATCC CTGTGACCCC TCCCCAGTGC CTCTCCTGGC CCTGGAAGTT 
     4921 GCCACTCCAG TGCCCACCAG CCTTGTCCTA ATAAAATTAA GTTGCATCAT TTTGTCTGAC 
     4981 TAGGTGTCCT TCTATAATAT TATGGGGTGG AGGGGGGTGG TATGGAGCAA GGGGCAAGTT 
     5041 GGGAAGACAA CCTGTAGGGC CTGCGGGGTC TATTGGGAAC CAAGCTGGAG TGCAGTGGCA 
     5101 CAATCTTGGC TCACTGCAAT CTCCGCCTCC TGGGTTCAAG CGATTCTCCT GCCTCAGCCT 
     5161 CCCGAGTTGT TGGGATTCCA GGCATGCATG ACCAGGCTCA GCTAATTTTT GTTTTTTTGG 
     5221 TAGAGACGGG GTTTCACCAT ATTGGCCAGG CTGGTCTCCA ACTCCTAATC TCAGGTGATC 
     5281 TACCCACCTT GGCCTCCCAA ATTGCTGGGA TTACAGGCGT GAACCACTGC TCCCTTCCCT 
     5341 GTCCTTCTGA TTTTAAAATA ACTATACCAG CAGGAGGACG TCCAGACACA GCATAGGCTA 
     5401 CCTGGCCATG CCCAACCGGT GGGACATTTG AGTTGCTTGC TTGGCACTGT CCTCTCATGC 
     5461 GTTGGGTCCA CTCAGTAGAT GCCTGTTGAA TTGGGTACGC GGCCAGCGGC GAGCGGTATC 
     5521 AGCTCACTCA AAGGCGGTAA TACGGTTATC CACAGAATCA GGGGATAACG CAGGAAAGAA 
     5581 CATGTGAGCA AAAGGCCAGC AAAAGGCCAG GAACCGTAAA AAGGCCGCGT TGCTGGCGTT 
     5641 TTTCCATAGG CTCCGCCCCC CTGACGAGCA TCACAAAAAT CGACGCTCAA GTCAGAGGTG 
     5701 GCGAAACCCG ACAGGACTAT AAAGATACCA GGCGTTTCCC CCTGGAAGCT CCCTCGTGCG 
     5761 CTCTCCTGTT CCGACCCTGC CGCTTACCGG ATACCTGTCC GCCTTTCTCC CTTCGGGAAG 
     5821 CGTGGCGCTT TCTCATAGCT CACGCTGTAG GTATCTCAGT TCGGTGTAGG TCGTTCGCTC 
     5881 CAAGCTGGGC TGTGTGCACG AACCCCCCGT TCAGCCCGAC CGCTGCGCCT TATCCGGTAA 
     5941 CTATCGTCTT GAGTCCAACC CGGTAAGACA CGACTTATCG CCACTGGCAG CAGCCACTGG 
     6001 TAACAGGATT AGCAGAGCGA GGTATGTAGG CGGTGCTACA GAGTTCTTGA AGTGGTGGCC 
     6061 TAACTACGGC TACACTAGAA GAACAGTATT TGGTATCTGC GCTCTGCTGA AGCCAGTTAC 
     6121 CTTCGGAAAA AGAGTTGGTA GCTCTTGATC CGGCAAACAA ACCACCGCTG GTAGCGGTGG 
     6181 TTTTTTTGTT TGCAAGCAGC AGATTACGCG CAGAAAAAAA GGATCTCAAG AAGATCCTTT 
     6241 GATCTTTTCT ACGGGGTCTG ACGCTCAGTG GAACGAAAAC TCACGTTAAG GGATTTTGGT 
     6301 CATGAGATTA TCAAAAAGGA TCTTCACCTA GATCCTTTTA AATTAAAAAT GAAGTTTTAA 
     6361 ATCAATCTAA AGTATATATG AGTAACCTGA GGCTATGGCA GGGCCTGCCG CCCCGACGTT 
     6421 GGCTGCGAGC CCTGGGCCTT CACCCGAACT TGGGGGGTGG GGTGGGGAAA AGGAAGAAAC 
     6481 GCGGGCGTAT TGGCCCCAAT GGGGTCTCGG TGGGGTATCG ACAGAGTGCC AGCCCTGGGA 
     6541 CCGAACCCCG CGTTTATGAA CAAACGACCC AACACCGTGC GTTTTATTCT GTCTTTTTAT 
     6601 TGCCGTCATA GCGCGGGTTC CTTCCGGTAT TGTCTCCTTC CGTGTTTCAG TTAGCCTCCC 
     6661 CCTAGGGTGG GCGAAGAACT CCAGCATGAG ATCCCCGCGC TGGAGGATCA TCCAGCCGGC 
     6721 GTCCCGGAAA ACGATTCCGA AGCCCAACCT TTCATAGAAG GCGGCGGTGG AATCGAAATC 
     6781 TCGTGATGGC AGGTTGGGCG TCGCTTGGTC GGTCATTTCG AACCCCAGAG TCCCGCTCAG 
     6841 AAGAACTCGT CAAGAAGGCG ATAGAAGGCG ATGCGCTGCG AATCGGGAGC GGCGATACCG 
     6901 TAAAGCACGA GGAAGCGGTC AGCCCATTCG CCGCCAAGCT CTTCAGCAAT ATCACGGGTA 
     6961 GCCAACGCTA TGTCCTGATA GCGATCCGCC ACACCCAGCC GGCCACAGTC GATGAATCCA 
     7021 GAAAAGCGGC CATTTTCCAC CATGATATTC GGCAAGCAGG CATCGCCATG GGTCACGACG 
     7081 AGATCCTCGC CGTCGGGCAT GCTCGCCTTG AGCCTGGCGA ACAGTTCGGC TGGCGCGAGC 
     7141 CCCTGATGCT CTTCGTCCAG ATCATCCTGA TCGACAAGAC CGGCTTCCAT CCGAGTACGT 
     7201 GCTCGCTCGA TGCGATGTTT CGCTTGGTGG TCGAATGGGC AGGTAGCCGG ATCAAGCGTA 
     7261 TGCAGCCGCC GCATTGCATC AGCCATGATG GATACTTTCT CGGCAGGAGC AAGGTGAGAT 
     7321 GACAGGAGAT CCTGCCCCGG CACTTCGCCC AATAGCAGCC AGTCCCTTCC CGCTTCAGTG 
     7381 ACAACGTCGA GCACAGCTGC GCAAGGAACG CCCGTCGTGG CCAGCCACGA TAGCCGCGCT 
     7441 GCCTCGTCTT GCAGTTCATT CAGGGCACCG GACAGGTCGG TCTTGACAAA AAGAACCGGG 
     7501 CGCCCCTGCG CTGACAGCCG GAACACGGCG GCATCAGAGC AGCCGATTGT CTGTTGTGCC 
     7561 CAGTCATAGC CGAATAGCCT CTCCACCCAA GCGGCCGGAG AACCTGCGTG CAATCCATCT 
     7621 TGTTCAATCA TGCGAAACGA TCCTCATCCT GTCTCTTGAT CGATCTTTGC AAAAGCCTAG 
     7681 GCCTCCAAAA AAGCCTCCTC ACTACTTCTG GAATAGCTCA GAGGCCGAGG CGGCCTCGGC 
     7741 CTCTGCATAA ATAAAAAAAA TTAGTCAGCC ATGGGGCGGA GAATGGGCGG AACTGGGCGG 
     7801 AGTTAGGGGC GGGATGGGCG GAGTTAGGGG CGGGACTATG GTTGCTGACT AATTGAGATG 
     7861 CATGCTTTGC ATACTTCTGC CTGCTGGGGA GCCTGGGGAC TTTCCACACC TGGTTGCTGA 
     7921 CTAATTGAGA TGCATGCTTT GCATACTTCT GCCTGCTGGG GAGCCTGGGG ACTTTCCACA 
     7981 CCCTAACTGA CACACATTCC ACAGCTGGTT CTTTCCGCCT CAGGACTCTT CCTTTTTCAA 
     8041 TATTATTGAA GCATTTATCA GGGTTATTGT CTCATGAGCG GATACATATT TGAATGTATT 
     8101 TAGAAAAATA AACAAATAGG GGTTCCGCGC ACATTTCCCC GAAAAGTGCC ACCTGACGCG 
     8161 CCCTGTAGCG GCGCATTAAG CGCGGCGGGT GTGGTGGTTA CGCGCAGCGT GACCGCTACA 
     8221 CTTGCCAGCG CCCTAGCGCC CGCTCCTTTC GCTTTCTTCC CTTCCTTTCT CGCCACGTTC 
     8281 GCCGGCTTTC CCCGTCAAGC TCTAAATCGG GGGCTCCCTT TAGGGTTCCG ATTTAGTGCT 
     8341 TTACGGCACC TCGACCCCAA AAAACTTGAT TAGGGTGATG GTTCACGTAG TGGGCCATCG 
     8401 CCCTGATAGA CGGTTTTTCG CCCTTTGACG TTGGAGTCCA CGTTCTTTAA TAGTGGACTC 
     8461 TTGTTCCAAA CTGGAACAAC ACTCAACCCT ATCTCGGTCT ATTCTTTTGA TTTATAAGGG 
     8521 ATTTTGCCGA TTTCGGCCTA TTGGTTAAAA AATGAGCTGA TTTAACAAAA ATTTAACGCG 
     8581 AATTTT
//