LOCUS       RC214294                7419 bp    DNA     circular  SYN 
DEFINITION  EXO1 (Myc-DDK-tagged)-Human exonuclease 1 (EXO1), transcript variant 1
ACCESSION   NM_006027
VERSION     NM_006027.3
SOURCE      synthetic construct 
FEATURES    Location/Qualifiers 
     misc_feature    201..924 
                     /label="CMV promoter"  
     misc_feature    839..856 
                     /label="Restriction Enzyme"  
     misc_feature    953..971 
                     /label="T7 promoter"  
     misc_feature    3576..3667 
                     /label="Myc-DDK"  
     misc_feature    3697..4282 
                     /label="PolyA signal"  
     misc_feature    3754..3773 
                     /label="Restriction Enzyme"  
     misc_feature    4558..5230 
                     /label="ColE1"  
     misc_feature    5660..6454 
                     /label="Kan/Neo"  
     misc_feature    6489..6791 
                     /label="SV40 ori"  
     misc_feature    6877..7354 
                     /label="f1 ori"  
     misc_feature    1020..1028 
                     /label="Restriction Enzyme"  
     misc_feature    3558..3564 
                     /label="Restriction Enzyme"  
     misc_feature    1020..3558 
                     /label="EXO1"  
ORIGIN                      
        1 AACAAAATAT TAACGCTTAC AATTTCCATT CGCCATTCAG GCTGCGCAAC TGTTGGGAAG 
       61 GGCGATCGGT GCGGGCCTCT TCGCTATTAC GCCAGCTGGC GAAAGGGGGA TGTGCTGCAA 
      121 GGCGATTAAG TTGGGTAACG CCAGGGTTTT CCCAGTCACG ACGTTGTAAA ACGACGGCCA 
      181 GTGCCAAGCT GATCTATACA TTGAATCAAT ATTGGCAATT AGCCATATTA GTCATTGGTT 
      241 ATATAGCATA AATCAATATT GGCTATTGGC CATTGCATAC GTTGTATCTA TATCATAATA 
      301 TGTACATTTA TATTGGCTCA TGTCCAATAT GACCGCCATG TTGACATTGA TTATTGACTA 
      361 GTTATTAATA GTAATCAATT ACGGGGTCAT TAGTTCATAG CCCATATATG GAGTTCCGCG 
      421 TTACATAACT TACGGTAAAT GGCCCGCCTG GCTGACCGCC CAACGACCCC CGCCCATTGA 
      481 CGTCAATAAT GACGTATGTT CCCATAGTAA CGCCAATAGG GACTTTCCAT TGACGTCAAT 
      541 GGGTGGAGTA TTTACGGTAA ACTGCCCACT TGGCAGTACA TCAAGTGTAT CATATGCCAA 
      601 GTCCGCCCCC TATTGACGTC AATGACGGTA AATGGCCCGC CTGGCATTAT GCCCAGTACA 
      661 TGACCTTACG GGACTTTCCT ACTTGGCAGT ACATCTACGT ATTAGTCATC GCTATTACCA 
      721 TGGTGATGCG GTTTTGGCAG TACACCAATG GGCGTGGATA GCGGTTTGAC TCACGGGGAT 
      781 TTCCAAGTCT CCACCCCATT GACGTCAATG GGAGTTTGTT TTGGCACCAA AATCAACGGG 
      841 ACTTTCCAAA ATGTCGTAAT AACCCCGCCC CGTTGACGCA AATGGGCGGT AGGCGTGTAC 
      901 GGTGGGAGGT CTATATAAGC AGAGCTCGTT TAGTGAACCG TCAGAATTTT GTAATACGAC 
      961 TCACTATAGG GCGGCCGGGA ATTCGTCGAC TGGATCCGGT ACCGAGGAGA TCTGCCGCCG 
     1021 CGATCGCCAT GGGGATACAG GGATTGCTAC AATTTATCAA AGAAGCTTCA GAACCCATCC 
     1081 ATGTGAGGAA GTATAAAGGG CAGGTAGTAG CTGTGGATAC ATATTGCTGG CTTCACAAAG 
     1141 GAGCTATTGC TTGTGCTGAA AAACTAGCCA AAGGTGAACC TACTGATAGG TATGTAGGAT 
     1201 TTTGTATGAA ATTTGTAAAT ATGTTACTAT CTCATGGGAT CAAGCCTATT CTCGTATTTG 
     1261 ATGGATGTAC TTTACCTTCT AAAAAGGAAG TAGAGAGATC TAGAAGAGAA AGACGACAAG 
     1321 CCAATCTTCT TAAGGGAAAG CAACTTCTTC GTGAGGGGAA AGTCTCGGAA GCTCGAGAGT 
     1381 GTTTCACCCG GTCTATCAAT ATCACACATG CCATGGCCCA CAAAGTAATT AAAGCTGCCC 
     1441 GGTCTCAGGG GGTAGATTGC CTCGTGGCTC CCTATGAAGC TGATGCGCAG TTGGCCTATC 
     1501 TTAACAAAGC GGGAATTGTG CAAGCCATAA TTACAGAGGA CTCGGATCTC CTAGCTTTTG 
     1561 GCTGTAAAAA GGTAATTTTA AAGATGGACC AGTTTGGAAA TGGACTTGAA ATTGATCAAG 
     1621 CTCGGCTAGG AATGTGCAGA CAGCTTGGGG ATGTATTCAC GGAAGAGAAG TTTCGTTACA 
     1681 TGTGTATTCT TTCAGGTTGT GACTACCTGT CATCACTGCG TGGGATTGGA TTAGCAAAGG 
     1741 CATGCAAAGT CCTAAGACTA GCCAATAATC CAGATATAGT AAAGGTTATC AAGAAAATTG 
     1801 GACATTATCT CAAGATGAAT ATCACGGTAC CAGAGGATTA CATCAACGGG TTTATTCGGG 
     1861 CCAACAATAC CTTCCTCTAT CAGCTAGTTT TTGATCCCAT CAAAAGGAAA CTTATTCCTC 
     1921 TGAACGCCTA TGAAGATGAT GTTGATCCTG AAACACTAAG CTACGCTGGG CAATATGTTG 
     1981 ATGATTCCAT AGCTCTTCAA ATAGCACTTG GAAATAAAGA TATAAATACT TTTGAACAGA 
     2041 TCGATGACTA CAATCCAGAC ACTGCTATGC CTGCCCATTC AAGAAGTCAT AGTTGGGATG 
     2101 ACAAAACATG TCAAAAGTCA GCTAATGTTA GCAGCATTTG GCATAGGAAT TACTCTCCCA 
     2161 GACCAGAGTC GGGTACTGTT TCAGATGCCC CACAATTGAA GGAAAATCCA AGTACTGTGG 
     2221 GAGTGGAACG AGTGATTAGT ACTAAAGGGT TAAATCTCCC AAGGAAATCA TCCATTGTGA 
     2281 AAAGACCAAG AAGTGCAGAG CTGTCAGAAG ATGACCTGTT GAGTCAGTAT TCTCTTTCAT 
     2341 TTACGAAGAA GACCAAGAAA AATAGCTCTG AAGGCAATAA ATCATTGAGC TTTTCTGAAG 
     2401 TGTTTGTGCC TGACCTGGTA AATGGACCTA CTAACAAAAA GAGTGTAAGC ACTCCACCTA 
     2461 GGACGAGAAA TAAATTTGCA ACATTTTTAC AAAGGAAAAA TGAAGAAAGT GGTGCAGTTG 
     2521 TGGTTCCAGG GACCAGAAGC AGGTTTTTTT GCAGTTCAGA TTCTACTGAC TGTGTATCAA 
     2581 ACAAAGTGAG CATCCAGCCT CTGGATGAAA CTGCTGTCAC AGATAAAGAG AACAATCTGC 
     2641 ATGAATCAGA GTATGGAGAC CAAGAAGGCA AGAGACTGGT TGACACAGAT GTAGCACGTA 
     2701 ATTCAAGTGA TGACATTCCG AATAATCATA TTCCAGGTGA TCATATTCCA GACAAGGCAA 
     2761 CAGTGTTTAC AGATGAAGAG TCCTACTCTT TTGAGAGCAG CAAATTTACA AGGACCATTT 
     2821 CACCACCCAC TTTGGGAACA CTAAGAAGTT GTTTTAGTTG GTCTGGAGGT CTTGGAGATT 
     2881 TTTCAAGAAC GCCGAGCCCC TCTCCAAGCA CAGCATTGCA GCAGTTCCGA AGAAAGAGCG 
     2941 ATTCCCCCAC CTCTTTGCCT GAGAATAATA TGTCTGATGT GTCGCAGTTA AAGAGCGAGG 
     3001 AGTCCAGTGA CGATGAGTCT CATCCCTTAC GAGAAGGGGC ATGTTCTTCA CAGTCCCAGG 
     3061 AAAGTGGAGA ATTCTCACTG CAGAGTTCAA ATGCATCAAA GCTTTCTCAG TGCTCTAGTA 
     3121 AGGACTCTGA TTCAGAGGAA TCTGATTGCA ATATTAAGTT ACTTGACAGT CAAAGTGACC 
     3181 AGACCTCCAA GCTATGTTTA TCTCATTTCT CAAAAAAAGA CACACCTCTA AGGAACAAGG 
     3241 TTCCTGGGCT ATATAAGTCC AGTTCTGCAG ACTCTCTTTC TACAACCAAG ATCAAACCTC 
     3301 TAGGACCTGC CAGAGCCAGT GGGCTGAGCA AGAAGCCGGC AAGCATCCAG AAGAGAAAGC 
     3361 ATCATAATGC CGAGAACAAG CCGGGGTTAC AGATCAAACT CAATGAGCTC TGGAAAAACT 
     3421 TTGGATTTAA AAAAGATTCT GAAAAGCTTC CTCCTTGTAA GAAACCCCTG TCCCCAGTCA 
     3481 GAGATAACAT CCAACTAACT CCAGAAGCGG AAGAGGATAT ATTTAACAAA CCTGAATGTG 
     3541 GCCGTGTTCA AAGAGCAATA TTCCAGACGC GTACGCGGCC GCTCGAGCAG AAACTCATCT 
     3601 CAGAAGAGGA TCTGGCAGCA AATGATATCC TGGATTACAA GGATGACGAC GATAAGGTTT 
     3661 AAACGGCCGG CCGCGGTCAT AGCTGTTTCC TGAACAGATC CCGGGTGGCA TCCCTGTGAC 
     3721 CCCTCCCCAG TGCCTCTCCT GGCCCTGGAA GTTGCCACTC CAGTGCCCAC CAGCCTTGTC 
     3781 CTAATAAAAT TAAGTTGCAT CATTTTGTCT GACTAGGTGT CCTTCTATAA TATTATGGGG 
     3841 TGGAGGGGGG TGGTATGGAG CAAGGGGCAA GTTGGGAAGA CAACCTGTAG GGCCTGCGGG 
     3901 GTCTATTGGG AACCAAGCTG GAGTGCAGTG GCACAATCTT GGCTCACTGC AATCTCCGCC 
     3961 TCCTGGGTTC AAGCGATTCT CCTGCCTCAG CCTCCCGAGT TGTTGGGATT CCAGGCATGC 
     4021 ATGACCAGGC TCAGCTAATT TTTGTTTTTT TGGTAGAGAC GGGGTTTCAC CATATTGGCC 
     4081 AGGCTGGTCT CCAACTCCTA ATCTCAGGTG ATCTACCCAC CTTGGCCTCC CAAATTGCTG 
     4141 GGATTACAGG CGTGAACCAC TGCTCCCTTC CCTGTCCTTC TGATTTTAAA ATAACTATAC 
     4201 CAGCAGGAGG ACGTCCAGAC ACAGCATAGG CTACCTGGCC ATGCCCAACC GGTGGGACAT 
     4261 TTGAGTTGCT TGCTTGGCAC TGTCCTCTCA TGCGTTGGGT CCACTCAGTA GATGCCTGTT 
     4321 GAATTGGGTA CGCGGCCAGC GGCGAGCGGT ATCAGCTCAC TCAAAGGCGG TAATACGGTT 
     4381 ATCCACAGAA TCAGGGGATA ACGCAGGAAA GAACATGTGA GCAAAAGGCC AGCAAAAGGC 
     4441 CAGGAACCGT AAAAAGGCCG CGTTGCTGGC GTTTTTCCAT AGGCTCCGCC CCCCTGACGA 
     4501 GCATCACAAA AATCGACGCT CAAGTCAGAG GTGGCGAAAC CCGACAGGAC TATAAAGATA 
     4561 CCAGGCGTTT CCCCCTGGAA GCTCCCTCGT GCGCTCTCCT GTTCCGACCC TGCCGCTTAC 
     4621 CGGATACCTG TCCGCCTTTC TCCCTTCGGG AAGCGTGGCG CTTTCTCATA GCTCACGCTG 
     4681 TAGGTATCTC AGTTCGGTGT AGGTCGTTCG CTCCAAGCTG GGCTGTGTGC ACGAACCCCC 
     4741 CGTTCAGCCC GACCGCTGCG CCTTATCCGG TAACTATCGT CTTGAGTCCA ACCCGGTAAG 
     4801 ACACGACTTA TCGCCACTGG CAGCAGCCAC TGGTAACAGG ATTAGCAGAG CGAGGTATGT 
     4861 AGGCGGTGCT ACAGAGTTCT TGAAGTGGTG GCCTAACTAC GGCTACACTA GAAGAACAGT 
     4921 ATTTGGTATC TGCGCTCTGC TGAAGCCAGT TACCTTCGGA AAAAGAGTTG GTAGCTCTTG 
     4981 ATCCGGCAAA CAAACCACCG CTGGTAGCGG TGGTTTTTTT GTTTGCAAGC AGCAGATTAC 
     5041 GCGCAGAAAA AAAGGATCTC AAGAAGATCC TTTGATCTTT TCTACGGGGT CTGACGCTCA 
     5101 GTGGAACGAA AACTCACGTT AAGGGATTTT GGTCATGAGA TTATCAAAAA GGATCTTCAC 
     5161 CTAGATCCTT TTAAATTAAA AATGAAGTTT TAAATCAATC TAAAGTATAT ATGAGTAACC 
     5221 TGAGGCTATG GCAGGGCCTG CCGCCCCGAC GTTGGCTGCG AGCCCTGGGC CTTCACCCGA 
     5281 ACTTGGGGGG TGGGGTGGGG AAAAGGAAGA AACGCGGGCG TATTGGCCCC AATGGGGTCT 
     5341 CGGTGGGGTA TCGACAGAGT GCCAGCCCTG GGACCGAACC CCGCGTTTAT GAACAAACGA 
     5401 CCCAACACCG TGCGTTTTAT TCTGTCTTTT TATTGCCGTC ATAGCGCGGG TTCCTTCCGG 
     5461 TATTGTCTCC TTCCGTGTTT CAGTTAGCCT CCCCCTAGGG TGGGCGAAGA ACTCCAGCAT 
     5521 GAGATCCCCG CGCTGGAGGA TCATCCAGCC GGCGTCCCGG AAAACGATTC CGAAGCCCAA 
     5581 CCTTTCATAG AAGGCGGCGG TGGAATCGAA ATCTCGTGAT GGCAGGTTGG GCGTCGCTTG 
     5641 GTCGGTCATT TCGAACCCCA GAGTCCCGCT CAGAAGAACT CGTCAAGAAG GCGATAGAAG 
     5701 GCGATGCGCT GCGAATCGGG AGCGGCGATA CCGTAAAGCA CGAGGAAGCG GTCAGCCCAT 
     5761 TCGCCGCCAA GCTCTTCAGC AATATCACGG GTAGCCAACG CTATGTCCTG ATAGCGATCC 
     5821 GCCACACCCA GCCGGCCACA GTCGATGAAT CCAGAAAAGC GGCCATTTTC CACCATGATA 
     5881 TTCGGCAAGC AGGCATCGCC ATGGGTCACG ACGAGATCCT CGCCGTCGGG CATGCTCGCC 
     5941 TTGAGCCTGG CGAACAGTTC GGCTGGCGCG AGCCCCTGAT GCTCTTCGTC CAGATCATCC 
     6001 TGATCGACAA GACCGGCTTC CATCCGAGTA CGTGCTCGCT CGATGCGATG TTTCGCTTGG 
     6061 TGGTCGAATG GGCAGGTAGC CGGATCAAGC GTATGCAGCC GCCGCATTGC ATCAGCCATG 
     6121 ATGGATACTT TCTCGGCAGG AGCAAGGTGA GATGACAGGA GATCCTGCCC CGGCACTTCG 
     6181 CCCAATAGCA GCCAGTCCCT TCCCGCTTCA GTGACAACGT CGAGCACAGC TGCGCAAGGA 
     6241 ACGCCCGTCG TGGCCAGCCA CGATAGCCGC GCTGCCTCGT CTTGCAGTTC ATTCAGGGCA 
     6301 CCGGACAGGT CGGTCTTGAC AAAAAGAACC GGGCGCCCCT GCGCTGACAG CCGGAACACG 
     6361 GCGGCATCAG AGCAGCCGAT TGTCTGTTGT GCCCAGTCAT AGCCGAATAG CCTCTCCACC 
     6421 CAAGCGGCCG GAGAACCTGC GTGCAATCCA TCTTGTTCAA TCATGCGAAA CGATCCTCAT 
     6481 CCTGTCTCTT GATCGATCTT TGCAAAAGCC TAGGCCTCCA AAAAAGCCTC CTCACTACTT 
     6541 CTGGAATAGC TCAGAGGCCG AGGCGGCCTC GGCCTCTGCA TAAATAAAAA AAATTAGTCA 
     6601 GCCATGGGGC GGAGAATGGG CGGAACTGGG CGGAGTTAGG GGCGGGATGG GCGGAGTTAG 
     6661 GGGCGGGACT ATGGTTGCTG ACTAATTGAG ATGCATGCTT TGCATACTTC TGCCTGCTGG 
     6721 GGAGCCTGGG GACTTTCCAC ACCTGGTTGC TGACTAATTG AGATGCATGC TTTGCATACT 
     6781 TCTGCCTGCT GGGGAGCCTG GGGACTTTCC ACACCCTAAC TGACACACAT TCCACAGCTG 
     6841 GTTCTTTCCG CCTCAGGACT CTTCCTTTTT CAATATTATT GAAGCATTTA TCAGGGTTAT 
     6901 TGTCTCATGA GCGGATACAT ATTTGAATGT ATTTAGAAAA ATAAACAAAT AGGGGTTCCG 
     6961 CGCACATTTC CCCGAAAAGT GCCACCTGAC GCGCCCTGTA GCGGCGCATT AAGCGCGGCG 
     7021 GGTGTGGTGG TTACGCGCAG CGTGACCGCT ACACTTGCCA GCGCCCTAGC GCCCGCTCCT 
     7081 TTCGCTTTCT TCCCTTCCTT TCTCGCCACG TTCGCCGGCT TTCCCCGTCA AGCTCTAAAT 
     7141 CGGGGGCTCC CTTTAGGGTT CCGATTTAGT GCTTTACGGC ACCTCGACCC CAAAAAACTT 
     7201 GATTAGGGTG ATGGTTCACG TAGTGGGCCA TCGCCCTGAT AGACGGTTTT TCGCCCTTTG 
     7261 ACGTTGGAGT CCACGTTCTT TAATAGTGGA CTCTTGTTCC AAACTGGAAC AACACTCAAC 
     7321 CCTATCTCGG TCTATTCTTT TGATTTATAA GGGATTTTGC CGATTTCGGC CTATTGGTTA 
     7381 AAAAATGAGC TGATTTAACA AAAATTTAAC GCGAATTTT
//