LOCUS       RC214275                8685 bp    DNA     circular  SYN 
DEFINITION  MTR (Myc-DDK-tagged)-Human 5-methyltetrahydrofolate-homocysteine methyltransferase (MTR)
ACCESSION   NM_000254
VERSION     NM_000254.1
SOURCE      synthetic construct 
FEATURES    Location/Qualifiers 
     misc_feature    201..924 
                     /label="CMV promoter"  
     misc_feature    839..856 
                     /label="Restriction Enzyme"  
     misc_feature    953..971 
                     /label="T7 promoter"  
     misc_feature    4840..4931 
                     /label="Myc-DDK"  
     misc_feature    4961..5546 
                     /label="PolyA signal"  
     misc_feature    5018..5037 
                     /label="Restriction Enzyme"  
     misc_feature    5822..6494 
                     /label="ColE1"  
     misc_feature    6924..7718 
                     /label="Kan/Neo"  
     misc_feature    7753..8055 
                     /label="SV40 ori"  
     misc_feature    8141..8618 
                     /label="f1 ori"  
     misc_feature    1020..1028 
                     /label="Restriction Enzyme"  
     misc_feature    4815..4822 
                     /label="Restriction Enzyme"  
     misc_feature    1020..4815 
                     /label="MTR"  
ORIGIN                      
        1 AACAAAATAT TAACGCTTAC AATTTCCATT CGCCATTCAG GCTGCGCAAC TGTTGGGAAG 
       61 GGCGATCGGT GCGGGCCTCT TCGCTATTAC GCCAGCTGGC GAAAGGGGGA TGTGCTGCAA 
      121 GGCGATTAAG TTGGGTAACG CCAGGGTTTT CCCAGTCACG ACGTTGTAAA ACGACGGCCA 
      181 GTGCCAAGCT GATCTATACA TTGAATCAAT ATTGGCAATT AGCCATATTA GTCATTGGTT 
      241 ATATAGCATA AATCAATATT GGCTATTGGC CATTGCATAC GTTGTATCTA TATCATAATA 
      301 TGTACATTTA TATTGGCTCA TGTCCAATAT GACCGCCATG TTGACATTGA TTATTGACTA 
      361 GTTATTAATA GTAATCAATT ACGGGGTCAT TAGTTCATAG CCCATATATG GAGTTCCGCG 
      421 TTACATAACT TACGGTAAAT GGCCCGCCTG GCTGACCGCC CAACGACCCC CGCCCATTGA 
      481 CGTCAATAAT GACGTATGTT CCCATAGTAA CGCCAATAGG GACTTTCCAT TGACGTCAAT 
      541 GGGTGGAGTA TTTACGGTAA ACTGCCCACT TGGCAGTACA TCAAGTGTAT CATATGCCAA 
      601 GTCCGCCCCC TATTGACGTC AATGACGGTA AATGGCCCGC CTGGCATTAT GCCCAGTACA 
      661 TGACCTTACG GGACTTTCCT ACTTGGCAGT ACATCTACGT ATTAGTCATC GCTATTACCA 
      721 TGGTGATGCG GTTTTGGCAG TACACCAATG GGCGTGGATA GCGGTTTGAC TCACGGGGAT 
      781 TTCCAAGTCT CCACCCCATT GACGTCAATG GGAGTTTGTT TTGGCACCAA AATCAACGGG 
      841 ACTTTCCAAA ATGTCGTAAT AACCCCGCCC CGTTGACGCA AATGGGCGGT AGGCGTGTAC 
      901 GGTGGGAGGT CTATATAAGC AGAGCTCGTT TAGTGAACCG TCAGAATTTT GTAATACGAC 
      961 TCACTATAGG GCGGCCGGGA ATTCGTCGAC TGGATCCGGT ACCGAGGAGA TCTGCCGCCG 
     1021 CGATCGCCAT GTCACCCGCG CTCCAAGACC TGTCGCAACC CGAAGGTCTG AAGAAAACCC 
     1081 TGCGGGATGA GATCAATGCC ATTCTGCAGA AGAGGATTAT GGTGCTGGAT GGAGGGATGG 
     1141 GGACCATGAT CCAGCGGGAG AAGCTAAACG AAGAACACTT CCGAGGTCAG GAATTTAAAG 
     1201 ATCATGCCAG GCCGCTGAAA GGCAACAATG ACATTTTAAG TATAACTCAG CCTGATGTCA 
     1261 TTTACCAAAT CCATAAGGAA TACTTGCTGG CTGGGGCAGA TATCATTGAA ACAAATACTT 
     1321 TTAGCAGCAC TAGTATTGCC CAAGCTGACT ATGGCCTTGA ACACTTGGCC TACCGGATGA 
     1381 ACATGTGCTC TGCAGGAGTG GCCAGAAAAG CTGCCGAGGA GGTAACTCTC CAGACAGGAA 
     1441 TTAAGAGGTT TGTGGCAGGG GCTCTGGGTC CGACTAATAA GACACTCTCT GTGTCCCCAT 
     1501 CTGTGGAAAG GCCGGATTAT AGGAACATCA CATTTGATGA GCTTGTTGAA GCATACCAAG 
     1561 AGCAGGCCAA AGGACTTCTG GATGGCGGGG TTGATATCTT ACTCATTGAA ACTATTTTTG 
     1621 ATACTGCCAA TGCCAAGGCA GCCTTGTTTG CACTCCAAAA TCTTTTTGAG GAGAAATATG 
     1681 CTCCCCGGCC TATCTTTATT TCAGGGACGA TCGTTGATAA AAGTGGGCGG ACTCTTTCCG 
     1741 GACAGACAGG AGAGGGATTT GTCATCAGCG TGTCTCATGG AGAACCACTC TGCATTGGAT 
     1801 TAAATTGTGC TTTGGGTGCA GCTGAAATGA GACCTTTTAT TGAAATAATT GGAAAATGTA 
     1861 CAACAGCCTA TGTCCTCTGT TATCCCAATG CAGGTCTTCC CAACACCTTT GGTGACTATG 
     1921 ATGAAACGCC TTCTATGATG GCCAAGCACC TAAAGGATTT TGCTATGGAT GGCTTGGTCA 
     1981 ATATAGTTGG AGGATGCTGT GGGTCAACAC CAGATCATAT CAGGGAAATT GCTGAAGCTG 
     2041 TGAAAAATTG TAAGCCTAGA GTTCCACCTG CCACTGCTTT TGAAGGACAT ATGTTACTGT 
     2101 CTGGTCTAGA GCCCTTCAGG ATTGGACCGT ACACCAACTT TGTTAACATT GGAGAGCGCT 
     2161 GTAATGTTGC AGGATCAAGG AAGTTTGCTA AACTCATCAT GGCAGGAAAC TATGAAGAAG 
     2221 CCTTGTGTGT TGCCAAAGTG CAGGTGGAAA TGGGAGCCCA GGTGTTGGAT GTCAACATGG 
     2281 ATGATGGCAT GCTAGATGGT CCAAGTGCAA TGACCAGATT TTGCAACTTA ATTGCTTCCG 
     2341 AGCCAGACAT CGCAAAGGTA CCTTTGTGCA TCGACTCCTC CAATTTTGCT GTGATTGAAG 
     2401 CTGGGTTAAA GTGCTGCCAA GGGAAGTGCA TTGTCAATAG CATTAGTCTG AAGGAAGGAG 
     2461 AGGACGACTT CTTGGAGAAG GCCAGGAAGA TTAAAAAGTA TGGAGCTGCT ATGGTGGTCA 
     2521 TGGCTTTTGA TGAAGAAGGA CAGGCAACAG AAACAGACAC AAAAATCAGA GTGTGCACCC 
     2581 GGGCCTACCA TCTGCTTGTG AAAAAACTGG GCTTTAATCC AAATGACATT ATTTTTGACC 
     2641 CTAATATCCT AACCATTGGG ACTGGAATGG AGGAACACAA CTTGTATGCC ATTAATTTTA 
     2701 TCCATGCAAC AAAAGTCATT AAAGAAACAT TACCTGGAGC CAGAATAAGT GGAGGTCTTT 
     2761 CCAACTTGTC CTTCTCCTTC CGAGGAATGG AAGCCATTCG AGAAGCAATG CATGGGGTTT 
     2821 TCCTTTACCA TGCAATCAAG TCTGGCATGG ACATGGGGAT AGTGAATGCT GGAAACCTCC 
     2881 CTGTGTATGA TGATATCCAT AAGGAACTTC TGCAGCTCTG TGAAGATCTC ATCTGGAATA 
     2941 AAGACCCTGA GGCCACTGAG AAGCTCTTAC GTTATGCCCA GACTCAAGGC ACAGGAGGGA 
     3001 AGAAAGTCAT TCAGACTGAT GAGTGGAGAA ATGGCCCTGT CGAAGAACGC CTTGAGTATG 
     3061 CCCTTGTGAA GGGCATTGAA AAACATATTA TTGAGGATAC TGAGGAAGCC AGGTTAAACC 
     3121 AAAAAAAATA TCCCCGACCT CTCAATATAA TTGAAGGACC CCTGATGAAT GGAATGAAAA 
     3181 TTGTTGGTGA TCTTTTTGGA GCTGGAAAAA TGTTTCTACC TCAGGTTATA AAGTCAGCCC 
     3241 GGGTTATGAA GAAGGCTGTT GGCCACCTTA TCCCTTTCAT GGAAAAAGAA AGAGAAGAAA 
     3301 CCAGAGTGCT TAACGGCACA GTAGAAGAAG AGGACCCTTA CCAGGGCACC ATCGTGCTGG 
     3361 CCACTGTTAA AGGCGACGTG CACGACATAG GCAAGAACAT AGTTGGAGTA GTCCTTGGCT 
     3421 GCAATAATTT CCGAGTTATT GATTTAGGAG TCATGACTCC ATGTGATAAG ATACTGAAAG 
     3481 CTGCTCTTGA CCACAAAGCA GATATAATTG GCCTGTCAGG ACTCATCACT CCTTCCCTGG 
     3541 ATGAAATGAT TTTTGTTGCC AAGGAAATGG AGAGATTAGC TATAAGGATT CCATTGTTGA 
     3601 TTGGAGGAGC AACCACTTCA AAAACCCACA CAGCAGTTAA AATAGCTCCG AGATACAGTG 
     3661 CACCTGTAAT CCATGTCCTG GACGCGTCCA AGAGTGTGGT GGTGTGTTCC CAGCTGTTAG 
     3721 ATGAAAATCT AAAGGATGAA TACTTTGAGG AAATCATGGA AGAATATGAA GATATTAGAC 
     3781 AGGACCATTA TGAGTCTCTC AAGGAGAGGA GATACTTACC CTTAAGTCAA GCCAGAAAAA 
     3841 GTGGTTTCCA AATGGATTGG CTGTCTGAAC CTCACCCAGT GAAGCCCACG TTTATTGGGA 
     3901 CCCAGGTCTT TGAAGACTAT GACCTGCAGA AGCTGGTGGA CTACATTGAC TGGAAGCCTT 
     3961 TCTTTGATGT CTGGCAGCTC CGGGGCAAGT ACCCGAATCG AGGCTTTCCC AAGATATTTA 
     4021 ACGACAAAAC AGTAGGTGGA GAGGCCAGGA AGGTCTACGA TGATGCCCAC AATATGCTGA 
     4081 ACACACTGAT TAGTCAAAAG AAACTCCGGG CCCGGGGTGT GGTTGGGTTC TGGCCAGCAC 
     4141 AGAGTATCCA AGACGACATT CACCTGTACG CAGAGGCTGC TGTGCCCCAG GCTGCAGAGC 
     4201 CCATAGCCAC CTTCTATGGG TTAAGGCAAC AGGCTGAGAA GGACTCTGCC AGCACGGAGC 
     4261 CATACTACTG CCTCTCAGAC TTCATCGCTC CCTTGCATTC TGGCATCCGT GACTACCTGG 
     4321 GCCTGTTTGC CGTTGCCTGC TTTGGGGTAG AAGAGCTGAG CAAGGCCTAT GAGGATGATG 
     4381 GTGACGACTA CAGCAGCATC ATGGTCAAGG CGCTGGGGGA CCGGCTGGCA GAGGCCTTTG 
     4441 CAGAAGAGCT CCATGAAAGA GTTCGCCGAG AACTGTGGGC CTACTGTGGC AGTGAGCAGC 
     4501 TGGACGTCGC AGACCTGCGC AGGCTGCGGT ACAAGGGCAT CCGCCCGGCT CCTGGCTACC 
     4561 CCAGCCAGCC CGACCACACC GAGAAGCTCA CCATGTGGAG ACTCGCAGAC ATCGAGCAGT 
     4621 CTACAGGCAT TAGGTTAACA GAATCATTAG CAATGGCACC TGCTTCAGCA GTCTCAGGCC 
     4681 TCTACTTCTC CAATTTGAAG TCCAAATATT TTGCTGTGGG GAAGATTTCC AAGGATCAGG 
     4741 TTGAGGATTA TGCATTGAGG AAGAACATAT CTGTGGCTGA GGTTGAGAAA TGGCTTGGAC 
     4801 CCATTTTGGG ATATGATACA GACAGCGGAC CGACGCGTAC GCGGCCGCTC GAGCAGAAAC 
     4861 TCATCTCAGA AGAGGATCTG GCAGCAAATG ATATCCTGGA TTACAAGGAT GACGACGATA 
     4921 AGGTTTAAAC GGCCGGCCGC GGTCATAGCT GTTTCCTGAA CAGATCCCGG GTGGCATCCC 
     4981 TGTGACCCCT CCCCAGTGCC TCTCCTGGCC CTGGAAGTTG CCACTCCAGT GCCCACCAGC 
     5041 CTTGTCCTAA TAAAATTAAG TTGCATCATT TTGTCTGACT AGGTGTCCTT CTATAATATT 
     5101 ATGGGGTGGA GGGGGGTGGT ATGGAGCAAG GGGCAAGTTG GGAAGACAAC CTGTAGGGCC 
     5161 TGCGGGGTCT ATTGGGAACC AAGCTGGAGT GCAGTGGCAC AATCTTGGCT CACTGCAATC 
     5221 TCCGCCTCCT GGGTTCAAGC GATTCTCCTG CCTCAGCCTC CCGAGTTGTT GGGATTCCAG 
     5281 GCATGCATGA CCAGGCTCAG CTAATTTTTG TTTTTTTGGT AGAGACGGGG TTTCACCATA 
     5341 TTGGCCAGGC TGGTCTCCAA CTCCTAATCT CAGGTGATCT ACCCACCTTG GCCTCCCAAA 
     5401 TTGCTGGGAT TACAGGCGTG AACCACTGCT CCCTTCCCTG TCCTTCTGAT TTTAAAATAA 
     5461 CTATACCAGC AGGAGGACGT CCAGACACAG CATAGGCTAC CTGGCCATGC CCAACCGGTG 
     5521 GGACATTTGA GTTGCTTGCT TGGCACTGTC CTCTCATGCG TTGGGTCCAC TCAGTAGATG 
     5581 CCTGTTGAAT TGGGTACGCG GCCAGCGGCG AGCGGTATCA GCTCACTCAA AGGCGGTAAT 
     5641 ACGGTTATCC ACAGAATCAG GGGATAACGC AGGAAAGAAC ATGTGAGCAA AAGGCCAGCA 
     5701 AAAGGCCAGG AACCGTAAAA AGGCCGCGTT GCTGGCGTTT TTCCATAGGC TCCGCCCCCC 
     5761 TGACGAGCAT CACAAAAATC GACGCTCAAG TCAGAGGTGG CGAAACCCGA CAGGACTATA 
     5821 AAGATACCAG GCGTTTCCCC CTGGAAGCTC CCTCGTGCGC TCTCCTGTTC CGACCCTGCC 
     5881 GCTTACCGGA TACCTGTCCG CCTTTCTCCC TTCGGGAAGC GTGGCGCTTT CTCATAGCTC 
     5941 ACGCTGTAGG TATCTCAGTT CGGTGTAGGT CGTTCGCTCC AAGCTGGGCT GTGTGCACGA 
     6001 ACCCCCCGTT CAGCCCGACC GCTGCGCCTT ATCCGGTAAC TATCGTCTTG AGTCCAACCC 
     6061 GGTAAGACAC GACTTATCGC CACTGGCAGC AGCCACTGGT AACAGGATTA GCAGAGCGAG 
     6121 GTATGTAGGC GGTGCTACAG AGTTCTTGAA GTGGTGGCCT AACTACGGCT ACACTAGAAG 
     6181 AACAGTATTT GGTATCTGCG CTCTGCTGAA GCCAGTTACC TTCGGAAAAA GAGTTGGTAG 
     6241 CTCTTGATCC GGCAAACAAA CCACCGCTGG TAGCGGTGGT TTTTTTGTTT GCAAGCAGCA 
     6301 GATTACGCGC AGAAAAAAAG GATCTCAAGA AGATCCTTTG ATCTTTTCTA CGGGGTCTGA 
     6361 CGCTCAGTGG AACGAAAACT CACGTTAAGG GATTTTGGTC ATGAGATTAT CAAAAAGGAT 
     6421 CTTCACCTAG ATCCTTTTAA ATTAAAAATG AAGTTTTAAA TCAATCTAAA GTATATATGA 
     6481 GTAACCTGAG GCTATGGCAG GGCCTGCCGC CCCGACGTTG GCTGCGAGCC CTGGGCCTTC 
     6541 ACCCGAACTT GGGGGGTGGG GTGGGGAAAA GGAAGAAACG CGGGCGTATT GGCCCCAATG 
     6601 GGGTCTCGGT GGGGTATCGA CAGAGTGCCA GCCCTGGGAC CGAACCCCGC GTTTATGAAC 
     6661 AAACGACCCA ACACCGTGCG TTTTATTCTG TCTTTTTATT GCCGTCATAG CGCGGGTTCC 
     6721 TTCCGGTATT GTCTCCTTCC GTGTTTCAGT TAGCCTCCCC CTAGGGTGGG CGAAGAACTC 
     6781 CAGCATGAGA TCCCCGCGCT GGAGGATCAT CCAGCCGGCG TCCCGGAAAA CGATTCCGAA 
     6841 GCCCAACCTT TCATAGAAGG CGGCGGTGGA ATCGAAATCT CGTGATGGCA GGTTGGGCGT 
     6901 CGCTTGGTCG GTCATTTCGA ACCCCAGAGT CCCGCTCAGA AGAACTCGTC AAGAAGGCGA 
     6961 TAGAAGGCGA TGCGCTGCGA ATCGGGAGCG GCGATACCGT AAAGCACGAG GAAGCGGTCA 
     7021 GCCCATTCGC CGCCAAGCTC TTCAGCAATA TCACGGGTAG CCAACGCTAT GTCCTGATAG 
     7081 CGATCCGCCA CACCCAGCCG GCCACAGTCG ATGAATCCAG AAAAGCGGCC ATTTTCCACC 
     7141 ATGATATTCG GCAAGCAGGC ATCGCCATGG GTCACGACGA GATCCTCGCC GTCGGGCATG 
     7201 CTCGCCTTGA GCCTGGCGAA CAGTTCGGCT GGCGCGAGCC CCTGATGCTC TTCGTCCAGA 
     7261 TCATCCTGAT CGACAAGACC GGCTTCCATC CGAGTACGTG CTCGCTCGAT GCGATGTTTC 
     7321 GCTTGGTGGT CGAATGGGCA GGTAGCCGGA TCAAGCGTAT GCAGCCGCCG CATTGCATCA 
     7381 GCCATGATGG ATACTTTCTC GGCAGGAGCA AGGTGAGATG ACAGGAGATC CTGCCCCGGC 
     7441 ACTTCGCCCA ATAGCAGCCA GTCCCTTCCC GCTTCAGTGA CAACGTCGAG CACAGCTGCG 
     7501 CAAGGAACGC CCGTCGTGGC CAGCCACGAT AGCCGCGCTG CCTCGTCTTG CAGTTCATTC 
     7561 AGGGCACCGG ACAGGTCGGT CTTGACAAAA AGAACCGGGC GCCCCTGCGC TGACAGCCGG 
     7621 AACACGGCGG CATCAGAGCA GCCGATTGTC TGTTGTGCCC AGTCATAGCC GAATAGCCTC 
     7681 TCCACCCAAG CGGCCGGAGA ACCTGCGTGC AATCCATCTT GTTCAATCAT GCGAAACGAT 
     7741 CCTCATCCTG TCTCTTGATC GATCTTTGCA AAAGCCTAGG CCTCCAAAAA AGCCTCCTCA 
     7801 CTACTTCTGG AATAGCTCAG AGGCCGAGGC GGCCTCGGCC TCTGCATAAA TAAAAAAAAT 
     7861 TAGTCAGCCA TGGGGCGGAG AATGGGCGGA ACTGGGCGGA GTTAGGGGCG GGATGGGCGG 
     7921 AGTTAGGGGC GGGACTATGG TTGCTGACTA ATTGAGATGC ATGCTTTGCA TACTTCTGCC 
     7981 TGCTGGGGAG CCTGGGGACT TTCCACACCT GGTTGCTGAC TAATTGAGAT GCATGCTTTG 
     8041 CATACTTCTG CCTGCTGGGG AGCCTGGGGA CTTTCCACAC CCTAACTGAC ACACATTCCA 
     8101 CAGCTGGTTC TTTCCGCCTC AGGACTCTTC CTTTTTCAAT ATTATTGAAG CATTTATCAG 
     8161 GGTTATTGTC TCATGAGCGG ATACATATTT GAATGTATTT AGAAAAATAA ACAAATAGGG 
     8221 GTTCCGCGCA CATTTCCCCG AAAAGTGCCA CCTGACGCGC CCTGTAGCGG CGCATTAAGC 
     8281 GCGGCGGGTG TGGTGGTTAC GCGCAGCGTG ACCGCTACAC TTGCCAGCGC CCTAGCGCCC 
     8341 GCTCCTTTCG CTTTCTTCCC TTCCTTTCTC GCCACGTTCG CCGGCTTTCC CCGTCAAGCT 
     8401 CTAAATCGGG GGCTCCCTTT AGGGTTCCGA TTTAGTGCTT TACGGCACCT CGACCCCAAA 
     8461 AAACTTGATT AGGGTGATGG TTCACGTAGT GGGCCATCGC CCTGATAGAC GGTTTTTCGC 
     8521 CCTTTGACGT TGGAGTCCAC GTTCTTTAAT AGTGGACTCT TGTTCCAAAC TGGAACAACA 
     8581 CTCAACCCTA TCTCGGTCTA TTCTTTTGAT TTATAAGGGA TTTTGCCGAT TTCGGCCTAT 
     8641 TGGTTAAAAA ATGAGCTGAT TTAACAAAAA TTTAACGCGA ATTTT
//