LOCUS       RC214261                8007 bp    DNA     circular  SYN 
DEFINITION  DAGLA (Myc-DDK-tagged)-Human diacylglycerol lipase, alpha (DAGLA)
ACCESSION   NM_006133
VERSION     NM_006133.2
SOURCE      synthetic construct 
FEATURES    Location/Qualifiers 
     misc_feature    201..924 
                     /label="CMV promoter"  
     misc_feature    839..856 
                     /label="Restriction Enzyme"  
     misc_feature    953..971 
                     /label="T7 promoter"  
     misc_feature    4164..4255 
                     /label="Myc-DDK"  
     misc_feature    4285..4870 
                     /label="PolyA signal"  
     misc_feature    4342..4361 
                     /label="Restriction Enzyme"  
     misc_feature    5146..5818 
                     /label="ColE1"  
     misc_feature    6248..7042 
                     /label="Kan/Neo"  
     misc_feature    7077..7379 
                     /label="SV40 ori"  
     misc_feature    7465..7942 
                     /label="f1 ori"  
     misc_feature    1020..1028 
                     /label="Restriction Enzyme"  
     misc_feature    4146..4152 
                     /label="Restriction Enzyme"  
     misc_feature    1020..4146 
                     /label="DAGLA"  
ORIGIN                      
        1 AACAAAATAT TAACGCTTAC AATTTCCATT CGCCATTCAG GCTGCGCAAC TGTTGGGAAG 
       61 GGCGATCGGT GCGGGCCTCT TCGCTATTAC GCCAGCTGGC GAAAGGGGGA TGTGCTGCAA 
      121 GGCGATTAAG TTGGGTAACG CCAGGGTTTT CCCAGTCACG ACGTTGTAAA ACGACGGCCA 
      181 GTGCCAAGCT GATCTATACA TTGAATCAAT ATTGGCAATT AGCCATATTA GTCATTGGTT 
      241 ATATAGCATA AATCAATATT GGCTATTGGC CATTGCATAC GTTGTATCTA TATCATAATA 
      301 TGTACATTTA TATTGGCTCA TGTCCAATAT GACCGCCATG TTGACATTGA TTATTGACTA 
      361 GTTATTAATA GTAATCAATT ACGGGGTCAT TAGTTCATAG CCCATATATG GAGTTCCGCG 
      421 TTACATAACT TACGGTAAAT GGCCCGCCTG GCTGACCGCC CAACGACCCC CGCCCATTGA 
      481 CGTCAATAAT GACGTATGTT CCCATAGTAA CGCCAATAGG GACTTTCCAT TGACGTCAAT 
      541 GGGTGGAGTA TTTACGGTAA ACTGCCCACT TGGCAGTACA TCAAGTGTAT CATATGCCAA 
      601 GTCCGCCCCC TATTGACGTC AATGACGGTA AATGGCCCGC CTGGCATTAT GCCCAGTACA 
      661 TGACCTTACG GGACTTTCCT ACTTGGCAGT ACATCTACGT ATTAGTCATC GCTATTACCA 
      721 TGGTGATGCG GTTTTGGCAG TACACCAATG GGCGTGGATA GCGGTTTGAC TCACGGGGAT 
      781 TTCCAAGTCT CCACCCCATT GACGTCAATG GGAGTTTGTT TTGGCACCAA AATCAACGGG 
      841 ACTTTCCAAA ATGTCGTAAT AACCCCGCCC CGTTGACGCA AATGGGCGGT AGGCGTGTAC 
      901 GGTGGGAGGT CTATATAAGC AGAGCTCGTT TAGTGAACCG TCAGAATTTT GTAATACGAC 
      961 TCACTATAGG GCGGCCGGGA ATTCGTCGAC TGGATCCGGT ACCGAGGAGA TCTGCCGCCG 
     1021 CGATCGCCAT GCCCGGGATC GTGGTGTTCC GGCGGCGCTG GTCTGTGGGC AGTGATGACC 
     1081 TCGTCCTACC GGCCATCTTC CTCTTTCTCC TGCATACCAC CTGGTTTGTG ATCCTGTCCG 
     1141 TGGTGCTCTT CGGCCTGGTC TATAACCCGC ACGAGGCCTG CTCCCTGAAC CTGGTGGACC 
     1201 ACGGCCGCGG CTACCTGGGC ATCCTGCTGA GCTGCATGAT CGCTGAGATG GCCATCATCT 
     1261 GGCTGAGCAT GCGCGGGGGC ATCCTCTACA CGGAGCCCCG TGACTCCATG CAGTACGTGC 
     1321 TCTACGTGCG CCTGGCCATC CTGGTGATCG AGTTCATCTA CGCCATCGTG GGCATCGTCT 
     1381 GGCTCACTCA GTACTACACC TCCTGCAACG ACCTCACTGC CAAGAATGTC ACCCTCGGAA 
     1441 TGGTTGTCTG CAACTGGGTA GTCATCCTCA GTGTGTGCAT CACTGTCCTC TGCGTCTTCG 
     1501 ACCCCACGGG CCGCACCTTT GTCAAGCTGA GAGCCACCAA GAGGAGGCAG CGTAACCTGC 
     1561 GGACCTACAA CCTGCGGCAC CGCTTAGAGG AGGGTCAAGC CACCAGCTGG TCGCGCCGGC 
     1621 TCAAAGTGTT CCTCTGCTGC ACGCGGACGA AGGACTCCCA GTCAGATGCC TACTCAGAAA 
     1681 TCGCCTACCT CTTTGCGGAG TTCTTCCGGG ACCTTGACAT TGTGCCATCC GACATCATTG 
     1741 CTGGCCTGGT GCTGCTCCGG CAGCGGCAGC GGGCCAAGCG CAACGCCGTG CTGGACGAGG 
     1801 CAAACAATGA CATCTTGGCC TTCCTGTCTG GGATGCCGGT GACCAGAAAC ACCAAGTACC 
     1861 TCGACCTCAA GAATTCACAA GAGATGCTCC GCTACAAAGA GGTCTGCTAC TACATGCTCT 
     1921 TTGCCCTGGC TGCCTACGGG TGGCCCATGT ACCTGATGCG GAAGCCCGCC TGCGGCCTCT 
     1981 GCCAACTGGC TCGGTCCTGC TCGTGTTGCC TGTGTCCTGC GAGGCCGCGG TTCGCCCCTG 
     2041 GAGTCACCAT CGAGGAAGAC AACTGCTGTG GCTGTAATGC CATTGCCATC CGGCGCCACT 
     2101 TCCTGGACGA GAACATGACT GCGGTGGACA TCGTCTATAC CTCCTGCCAT GATGCGGTCT 
     2161 ATGAAACGCC CTTCTACGTG GCGGTGGACC ATGACAAGAA GAAAGTGGTG ATCAGTATCC 
     2221 GGGGGACCCT GTCCCCCAAG GATGCCCTGA CTGACCTGAC GGGTGATGCT GAGCGCCTCC 
     2281 CCGTGGAGGG GCACCACGGC ACCTGGCTGG GCCACAAGGG TATGGTCCTC TCAGCTGAGT 
     2341 ACATCAAGAA GAAACTGGAG CAGGAGATGG TCCTGTCCCA GGCCTTTGGG CGAGACCTGG 
     2401 GCCGCGGAAC CAAACACTAC GGCCTGATTG TGGTGGGCCA CTCCCTGGGC GCGGGCACTG 
     2461 CTGCCATCCT CTCCTTCCTT CTGCGCCCAC AGTATCCGAC CCTCAAGTGC TTTGCCTACT 
     2521 CCCCGCCAGG GGGCCTGCTG AGTGAGGATG CGATGGAGTA TTCCAAGGAG TTCGTGACTG 
     2581 CTGTGGTTCT GGGCAAAGAC CTCGTCCCCA GGATTGGCCT CTCTCAGCTG GAAGGCTTCC 
     2641 GCAGACAGCT CCTGGATGTC CTGCAGCGAA GCACCAAGCC CAAATGGCGG ATCATCGTGG 
     2701 GGGCCACCAA ATGCATCCCC AAGTCGGAGC TGCCTGAGGA GGTAGAGGTG ACCACCCTGG 
     2761 CCAGCACGCG GCTCTGGACC CACCCCAGCG ACCTAACTAT AGCCCTCTCA GCCAGCACTC 
     2821 CACTCTACCC GCCCGGCCGC ATCATCCACG TGGTCCACAA CCACCCTGCA GAGCAGTGCT 
     2881 GCTGCTGTGA GCAGGAGGAG CCCACATACT TTGCCATCTG GGGCGACAAC AAGGCCTTCA 
     2941 ATGAGGTGAT CATCTCGCCA GCCATGCTGC ATGAGCACCT GCCCTATGTG GTCATGGAGG 
     3001 GGCTCAACAA GGTGCTGGAG AACTACAACA AGGGGAAGAC CGCTCTGCTC TCTGCAGCCA 
     3061 AGGTCATGGT GAGCCCTACC GAGGTGGACC TGACTCCTGA GCTCATCTTC CAGCAGCAGC 
     3121 CACTCCCCAC GGGGCCGCCC ATGCCCACTG GCCTTGCCCT GGAGCTGCCG ACTGCAGACC 
     3181 ACCGCAACAG CAGCGTCAGG AGCAAGTCCC AGTCTGAGAT GAGCCTGGAG GGCTTCTCGG 
     3241 AGGGGCGGCT GCTGTCGCCA GTGGTTGCGG CGGCGGCCCG CCAGGACCCG GTGGAGCTGC 
     3301 TGCTGCTGTC TACCCAGGAG CGGCTGGCGG CGGAGCTGCA GGCCCGGCGG GCACCACTGG 
     3361 CCACCATGGA GAGCCTCTCG GACACTGAGT CCCTGTACAG CTTCGACTCG CGCCGCTCCT 
     3421 CAGGCTTCCG CAGCATCCGG GGCTCCCCCA GCCTCCACGC TGTGCTGGAG CGTGATGAAG 
     3481 GCCACCTCTT CTACATTGAC CCTGCCATCC CCGAGGAAAA CCCATCCCTG AGCTCGCGCA 
     3541 CTGAGCTGCT GGCGGCCGAC AGCCTGTCCA AGCACTCACA GGACACGCAG CCCCTGGAGG 
     3601 CGGCCCTGGG CAGTGGCGGC GTCACTCCTG AGCGGCCCCC CAGTGCTGCG GCCAATGACG 
     3661 AGGAGGAAGA GGTTGGCGGT GGGGGTGGCG GGCCGGCCTC CCGCGGGGAG CTGGCGCTGC 
     3721 ACAATGGGCG CCTGGGGGAC TCGCCCAGTC CTCAGGTGCT GGAATTCGCC GAGTTCATCG 
     3781 ACAGCCTCTT CAACCTGGAC AGCAAGAGCA GCTCCTTCCA AGACCTCTAC TGCATGGTGG 
     3841 TGCCCGAGAG CCCCACCAGT GACTACGCTG AGGGCCCCAA GTCCCCCAGC CAGCAAGAGA 
     3901 TCCTGCTCCG TGCCCAGTTC GAGCCCAACC TGGTGCCCAA GCCCCCACGG CTCTTTGCCG 
     3961 GCTCAGCCGA CCCCTCCTCG GGCATCTCAC TCTCGCCCTC CTTCCCGCTC AGCTCCTCGG 
     4021 GTGAGCTCAT GGACCTGACG CCCACGGGCC TCAGTAGCCA GGAATGCCTG GCGGCTGACA 
     4081 AGATCCGGAC TTCTACCCCC ACTGGCCACG GAGCCAGCCC CGCCAAGCAA GATGAGCTGG 
     4141 TCATCTCAGC ACGCACGCGT ACGCGGCCGC TCGAGCAGAA ACTCATCTCA GAAGAGGATC 
     4201 TGGCAGCAAA TGATATCCTG GATTACAAGG ATGACGACGA TAAGGTTTAA ACGGCCGGCC 
     4261 GCGGTCATAG CTGTTTCCTG AACAGATCCC GGGTGGCATC CCTGTGACCC CTCCCCAGTG 
     4321 CCTCTCCTGG CCCTGGAAGT TGCCACTCCA GTGCCCACCA GCCTTGTCCT AATAAAATTA 
     4381 AGTTGCATCA TTTTGTCTGA CTAGGTGTCC TTCTATAATA TTATGGGGTG GAGGGGGGTG 
     4441 GTATGGAGCA AGGGGCAAGT TGGGAAGACA ACCTGTAGGG CCTGCGGGGT CTATTGGGAA 
     4501 CCAAGCTGGA GTGCAGTGGC ACAATCTTGG CTCACTGCAA TCTCCGCCTC CTGGGTTCAA 
     4561 GCGATTCTCC TGCCTCAGCC TCCCGAGTTG TTGGGATTCC AGGCATGCAT GACCAGGCTC 
     4621 AGCTAATTTT TGTTTTTTTG GTAGAGACGG GGTTTCACCA TATTGGCCAG GCTGGTCTCC 
     4681 AACTCCTAAT CTCAGGTGAT CTACCCACCT TGGCCTCCCA AATTGCTGGG ATTACAGGCG 
     4741 TGAACCACTG CTCCCTTCCC TGTCCTTCTG ATTTTAAAAT AACTATACCA GCAGGAGGAC 
     4801 GTCCAGACAC AGCATAGGCT ACCTGGCCAT GCCCAACCGG TGGGACATTT GAGTTGCTTG 
     4861 CTTGGCACTG TCCTCTCATG CGTTGGGTCC ACTCAGTAGA TGCCTGTTGA ATTGGGTACG 
     4921 CGGCCAGCGG CGAGCGGTAT CAGCTCACTC AAAGGCGGTA ATACGGTTAT CCACAGAATC 
     4981 AGGGGATAAC GCAGGAAAGA ACATGTGAGC AAAAGGCCAG CAAAAGGCCA GGAACCGTAA 
     5041 AAAGGCCGCG TTGCTGGCGT TTTTCCATAG GCTCCGCCCC CCTGACGAGC ATCACAAAAA 
     5101 TCGACGCTCA AGTCAGAGGT GGCGAAACCC GACAGGACTA TAAAGATACC AGGCGTTTCC 
     5161 CCCTGGAAGC TCCCTCGTGC GCTCTCCTGT TCCGACCCTG CCGCTTACCG GATACCTGTC 
     5221 CGCCTTTCTC CCTTCGGGAA GCGTGGCGCT TTCTCATAGC TCACGCTGTA GGTATCTCAG 
     5281 TTCGGTGTAG GTCGTTCGCT CCAAGCTGGG CTGTGTGCAC GAACCCCCCG TTCAGCCCGA 
     5341 CCGCTGCGCC TTATCCGGTA ACTATCGTCT TGAGTCCAAC CCGGTAAGAC ACGACTTATC 
     5401 GCCACTGGCA GCAGCCACTG GTAACAGGAT TAGCAGAGCG AGGTATGTAG GCGGTGCTAC 
     5461 AGAGTTCTTG AAGTGGTGGC CTAACTACGG CTACACTAGA AGAACAGTAT TTGGTATCTG 
     5521 CGCTCTGCTG AAGCCAGTTA CCTTCGGAAA AAGAGTTGGT AGCTCTTGAT CCGGCAAACA 
     5581 AACCACCGCT GGTAGCGGTG GTTTTTTTGT TTGCAAGCAG CAGATTACGC GCAGAAAAAA 
     5641 AGGATCTCAA GAAGATCCTT TGATCTTTTC TACGGGGTCT GACGCTCAGT GGAACGAAAA 
     5701 CTCACGTTAA GGGATTTTGG TCATGAGATT ATCAAAAAGG ATCTTCACCT AGATCCTTTT 
     5761 AAATTAAAAA TGAAGTTTTA AATCAATCTA AAGTATATAT GAGTAACCTG AGGCTATGGC 
     5821 AGGGCCTGCC GCCCCGACGT TGGCTGCGAG CCCTGGGCCT TCACCCGAAC TTGGGGGGTG 
     5881 GGGTGGGGAA AAGGAAGAAA CGCGGGCGTA TTGGCCCCAA TGGGGTCTCG GTGGGGTATC 
     5941 GACAGAGTGC CAGCCCTGGG ACCGAACCCC GCGTTTATGA ACAAACGACC CAACACCGTG 
     6001 CGTTTTATTC TGTCTTTTTA TTGCCGTCAT AGCGCGGGTT CCTTCCGGTA TTGTCTCCTT 
     6061 CCGTGTTTCA GTTAGCCTCC CCCTAGGGTG GGCGAAGAAC TCCAGCATGA GATCCCCGCG 
     6121 CTGGAGGATC ATCCAGCCGG CGTCCCGGAA AACGATTCCG AAGCCCAACC TTTCATAGAA 
     6181 GGCGGCGGTG GAATCGAAAT CTCGTGATGG CAGGTTGGGC GTCGCTTGGT CGGTCATTTC 
     6241 GAACCCCAGA GTCCCGCTCA GAAGAACTCG TCAAGAAGGC GATAGAAGGC GATGCGCTGC 
     6301 GAATCGGGAG CGGCGATACC GTAAAGCACG AGGAAGCGGT CAGCCCATTC GCCGCCAAGC 
     6361 TCTTCAGCAA TATCACGGGT AGCCAACGCT ATGTCCTGAT AGCGATCCGC CACACCCAGC 
     6421 CGGCCACAGT CGATGAATCC AGAAAAGCGG CCATTTTCCA CCATGATATT CGGCAAGCAG 
     6481 GCATCGCCAT GGGTCACGAC GAGATCCTCG CCGTCGGGCA TGCTCGCCTT GAGCCTGGCG 
     6541 AACAGTTCGG CTGGCGCGAG CCCCTGATGC TCTTCGTCCA GATCATCCTG ATCGACAAGA 
     6601 CCGGCTTCCA TCCGAGTACG TGCTCGCTCG ATGCGATGTT TCGCTTGGTG GTCGAATGGG 
     6661 CAGGTAGCCG GATCAAGCGT ATGCAGCCGC CGCATTGCAT CAGCCATGAT GGATACTTTC 
     6721 TCGGCAGGAG CAAGGTGAGA TGACAGGAGA TCCTGCCCCG GCACTTCGCC CAATAGCAGC 
     6781 CAGTCCCTTC CCGCTTCAGT GACAACGTCG AGCACAGCTG CGCAAGGAAC GCCCGTCGTG 
     6841 GCCAGCCACG ATAGCCGCGC TGCCTCGTCT TGCAGTTCAT TCAGGGCACC GGACAGGTCG 
     6901 GTCTTGACAA AAAGAACCGG GCGCCCCTGC GCTGACAGCC GGAACACGGC GGCATCAGAG 
     6961 CAGCCGATTG TCTGTTGTGC CCAGTCATAG CCGAATAGCC TCTCCACCCA AGCGGCCGGA 
     7021 GAACCTGCGT GCAATCCATC TTGTTCAATC ATGCGAAACG ATCCTCATCC TGTCTCTTGA 
     7081 TCGATCTTTG CAAAAGCCTA GGCCTCCAAA AAAGCCTCCT CACTACTTCT GGAATAGCTC 
     7141 AGAGGCCGAG GCGGCCTCGG CCTCTGCATA AATAAAAAAA ATTAGTCAGC CATGGGGCGG 
     7201 AGAATGGGCG GAACTGGGCG GAGTTAGGGG CGGGATGGGC GGAGTTAGGG GCGGGACTAT 
     7261 GGTTGCTGAC TAATTGAGAT GCATGCTTTG CATACTTCTG CCTGCTGGGG AGCCTGGGGA 
     7321 CTTTCCACAC CTGGTTGCTG ACTAATTGAG ATGCATGCTT TGCATACTTC TGCCTGCTGG 
     7381 GGAGCCTGGG GACTTTCCAC ACCCTAACTG ACACACATTC CACAGCTGGT TCTTTCCGCC 
     7441 TCAGGACTCT TCCTTTTTCA ATATTATTGA AGCATTTATC AGGGTTATTG TCTCATGAGC 
     7501 GGATACATAT TTGAATGTAT TTAGAAAAAT AAACAAATAG GGGTTCCGCG CACATTTCCC 
     7561 CGAAAAGTGC CACCTGACGC GCCCTGTAGC GGCGCATTAA GCGCGGCGGG TGTGGTGGTT 
     7621 ACGCGCAGCG TGACCGCTAC ACTTGCCAGC GCCCTAGCGC CCGCTCCTTT CGCTTTCTTC 
     7681 CCTTCCTTTC TCGCCACGTT CGCCGGCTTT CCCCGTCAAG CTCTAAATCG GGGGCTCCCT 
     7741 TTAGGGTTCC GATTTAGTGC TTTACGGCAC CTCGACCCCA AAAAACTTGA TTAGGGTGAT 
     7801 GGTTCACGTA GTGGGCCATC GCCCTGATAG ACGGTTTTTC GCCCTTTGAC GTTGGAGTCC 
     7861 ACGTTCTTTA ATAGTGGACT CTTGTTCCAA ACTGGAACAA CACTCAACCC TATCTCGGTC 
     7921 TATTCTTTTG ATTTATAAGG GATTTTGCCG ATTTCGGCCT ATTGGTTAAA AAATGAGCTG 
     7981 ATTTAACAAA AATTTAACGC GAATTTT
//