LOCUS       RC214253                8014 bp    DNA     circular  SYN 
DEFINITION  GNAS (Myc-DDK-tagged)-Human GNAS complex locus (GNAS), transcript variant 2
ACCESSION   NM_080425
VERSION     NM_080425.3
SOURCE      synthetic construct 
FEATURES    Location/Qualifiers 
     misc_feature    201..924 
                     /label="CMV promoter"  
     misc_feature    839..856 
                     /label="Restriction Enzyme"  
     misc_feature    953..971 
                     /label="T7 promoter"  
     misc_feature    4172..4263 
                     /label="Myc-DDK"  
     misc_feature    4293..4878 
                     /label="PolyA signal"  
     misc_feature    4350..4369 
                     /label="Restriction Enzyme"  
     misc_feature    5154..5826 
                     /label="ColE1"  
     misc_feature    6256..7050 
                     /label="Kan/Neo"  
     misc_feature    7085..7387 
                     /label="SV40 ori"  
     misc_feature    7473..7950 
                     /label="f1 ori"  
     misc_feature    1020..1028 
                     /label="Restriction Enzyme"  
     misc_feature    4131..4137 
                     /label="Restriction Enzyme"  
     misc_feature    1020..4131 
                     /label="GNAS"  
ORIGIN                      
        1 AACAAAATAT TAACGCTTAC AATTTCCATT CGCCATTCAG GCTGCGCAAC TGTTGGGAAG 
       61 GGCGATCGGT GCGGGCCTCT TCGCTATTAC GCCAGCTGGC GAAAGGGGGA TGTGCTGCAA 
      121 GGCGATTAAG TTGGGTAACG CCAGGGTTTT CCCAGTCACG ACGTTGTAAA ACGACGGCCA 
      181 GTGCCAAGCT GATCTATACA TTGAATCAAT ATTGGCAATT AGCCATATTA GTCATTGGTT 
      241 ATATAGCATA AATCAATATT GGCTATTGGC CATTGCATAC GTTGTATCTA TATCATAATA 
      301 TGTACATTTA TATTGGCTCA TGTCCAATAT GACCGCCATG TTGACATTGA TTATTGACTA 
      361 GTTATTAATA GTAATCAATT ACGGGGTCAT TAGTTCATAG CCCATATATG GAGTTCCGCG 
      421 TTACATAACT TACGGTAAAT GGCCCGCCTG GCTGACCGCC CAACGACCCC CGCCCATTGA 
      481 CGTCAATAAT GACGTATGTT CCCATAGTAA CGCCAATAGG GACTTTCCAT TGACGTCAAT 
      541 GGGTGGAGTA TTTACGGTAA ACTGCCCACT TGGCAGTACA TCAAGTGTAT CATATGCCAA 
      601 GTCCGCCCCC TATTGACGTC AATGACGGTA AATGGCCCGC CTGGCATTAT GCCCAGTACA 
      661 TGACCTTACG GGACTTTCCT ACTTGGCAGT ACATCTACGT ATTAGTCATC GCTATTACCA 
      721 TGGTGATGCG GTTTTGGCAG TACACCAATG GGCGTGGATA GCGGTTTGAC TCACGGGGAT 
      781 TTCCAAGTCT CCACCCCATT GACGTCAATG GGAGTTTGTT TTGGCACCAA AATCAACGGG 
      841 ACTTTCCAAA ATGTCGTAAT AACCCCGCCC CGTTGACGCA AATGGGCGGT AGGCGTGTAC 
      901 GGTGGGAGGT CTATATAAGC AGAGCTCGTT TAGTGAACCG TCAGAATTTT GTAATACGAC 
      961 TCACTATAGG GCGGCCGGGA ATTCGTCGAC TGGATCCGGT ACCGAGGAGA TCTGCCGCCG 
     1021 CGATCGCATG GGCGTGCGCA ACTGCCTCTA CGGCAATAAT ATGTCAGGAC AACGCGATAT 
     1081 CCCCCCTGAA ATCGGGGAAC AGCCCGAGCA ACCACCTTTG GAGGCCCCAG GGGCAGCTGC 
     1141 CCCCGGTGCT GGGCCTAGCC CAGCCGAAGA GATGGAGACC GAACCGCCTC ACAACGAGCC 
     1201 CATCCCCGTC GAGAATGATG GCGAGGCCTG TGGACCCCCA GAGGTCTCCA GACCCAACTT 
     1261 TCAGGTCCTC AACCCGGCAT TCAGGGAAGC TGGAGCCCAT GGAAGCTACA GCCCACCTCC 
     1321 TGAGGAAGCA ATGCCCTTCG AGGCTGAACA GCCCAGCTTG GGAGGCTTCT GGCCTACACT 
     1381 GGAGCAGCCT GGATTCCCCA GTGGGGTCCA TGCAGGCCTT GAGGCCTTCG GCCCAGCACT 
     1441 CATGGAGCCC GGAGCCTTCA GTGGTGCCAG ACCAGGCCTG GGAGGATACA GCCCTCCACC 
     1501 AGAAGAAGCT ATGCCCTTTG AGTTTGACCA GCCTGCCCAG AGAGGCTGCA GTCAACTTCT 
     1561 CTTACAGGTC CCAGACCTTG CTCCAGGAGG CCCAGGTGCT GCAGGGGTCC CCGGAGCTCC 
     1621 TCCCGAGGAG CCCCAAGCCC TCAGGCCTGC AAAGGCTGGC TCCAGAGGAG GCTACAGCCC 
     1681 TCCCCCTGAG GAGACTATGC CATTTGAGCT TGATGGAGAA GGATTTGGGG ACGACAGCCC 
     1741 ACCCCCGGGG CTTTCCCGAG TTATCGCACA AGTCGACGGC AGCAGCCAGT TCGCGGCAGT 
     1801 CGCGGCCTCG AGTGCGGTCC GCCTCACTCC CGCCGCGAAC GCGCCTCCCC TCTGGGTCCC 
     1861 AGGCGCCATC GGCAGCCCAT CCCAAGAGGC TGTCAGACCT CCTTCTAACT TCACGGGCAG 
     1921 CAGCCCCTGG ATGGAGATCT CCGGACCCCC GTTCGAGATT GGCAGCGCCC CCGCTGGGGT 
     1981 CGACGACACT CCCGTCAACA TGGACAGCCC CCCAATCGCG CTTGACGGCC CGCCCATCAA 
     2041 GGTCTCCGGA GCCCCAGATA AGAGAGAGCG AGCAGAGAGA CCCCCAGTTG AGGAGGAAGC 
     2101 AGCAGAGATG GAAGGAGCCG CTGATGCCGC GGAGGGAGGA AAAGTACCCT CTCCGGGGTA 
     2161 CGGATCCCCT GCCGCCGGGG CAGCCTCAGC GGATACCGCT GCCAGGGCAG CCCCTGCAGC 
     2221 CCCAGCCGAT CCTGACTCCG GGGCAACCCC AGAAGATCCC GACTCCGGGA CAGCACCAGC 
     2281 CGATCCTGAC TCCGGGGCAT TCGCAGCCGA TCCCGACTCC GGGGCAGCCC CTGCCGCCCC 
     2341 AGCCGATCCC GACTCCGGGG CGGCCCCTGA CGCCCCAGCC GATCCCGACT CCGGGGCGGC 
     2401 CCCTGACGCC CCAGCCGATC CAGATGCCGG GGCGGCCCCT GAGGCTCCCG CCGCCCCTGC 
     2461 GGCTGCTGAG ACCCGGGCAG CCCATGTCGC CCCAGCTGCG CCAGACGCAG GGGCTCCCAC 
     2521 TGCCCCAGCC GCTTCTGCCA CCCGGGCAGC CCAAGTCCGC CGGGCGGCCT CTGCAGCCCC 
     2581 TGCCTCCGGG GCCAGACGCA AGATCCATCT CAGACCCCCC AGCCCCGAGA TCCAGGCTGC 
     2641 CGATCCGCCT ACTCCGCGGC CTACTCGCGC GTCTGCCTGG CGGGGCAAGT CCGAGAGCAG 
     2701 CCGCGGCCGC CGCGTGTACT ACGATGAAGG GGTGGCCAGC AGCGACGATG ACTCCAGCGG 
     2761 AGACGAGTCC GACGATGGGA CCTCCGGATG CCTCCGCTGG TTTCAGCATC GGCGAAATCG 
     2821 CCGCCGCCGA AAGCCCCAGC GCAACTTACT CCGCAACTTT CTCGTGCAAG CCTTCGGGGG 
     2881 CTGCTTCGGT CGATCTGAGA GTCCCCAGCC CAAAGCCTCG CGCTCTCTCA AGGTCAAGAA 
     2941 GGTACCCCTG GCGGAGAAGC GCAGACAGAT GCGCAAAGAA GCCCTGGAGA AGCGGGCCCA 
     3001 GAAGCGCGCA GAGAAGAAAC GCAGTAAGCT CATCGACAAA CAACTCCAGG ACGAAAAGAT 
     3061 GGGCTACATG TGTACGCACC GCCTGCTGCT TCTAGGTGCT GGAGAATCTG GTAAAAGCAC 
     3121 CATTGTGAAG CAGATGAGGA TCCTGCATGT TAATGGGTTT AATGGAGAGG GCGGCGAAGA 
     3181 GGACCCGCAG GCTGCAAGGA GCAACAGCGA TGGTGAGAAG GCAACCAAAG TGCAGGACAT 
     3241 CAAAAACAAC CTGAAAGAGG CGATTGAAAC CATTGTGGCC GCCATGAGCA ACCTGGTGCC 
     3301 CCCCGTGGAG CTGGCCAACC CCGAGAACCA GTTCAGAGTG GACTACATCC TGAGTGTGAT 
     3361 GAACGTGCCT GACTTTGACT TCCCTCCCGA ATTCTATGAG CATGCCAAGG CTCTGTGGGA 
     3421 GGATGAAGGA GTGCGTGCCT GCTACGAACG CTCCAACGAG TACCAGCTGA TTGACTGTGC 
     3481 CCAGTACTTC CTGGACAAGA TCGACGTGAT CAAGCAGGCT GACTATGTGC CGAGCGATCA 
     3541 GGACCTGCTT CGCTGCCGTG TCCTGACTTC TGGAATCTTT GAGACCAAGT TCCAGGTGGA 
     3601 CAAAGTCAAC TTCCACATGT TTGACGTGGG TGGCCAGCGC GATGAACGCC GCAAGTGGAT 
     3661 CCAGTGCTTC AACGATGTGA CTGCCATCAT CTTCGTGGTG GCCAGCAGCA GCTACAACAT 
     3721 GGTCATCCGG GAGGACAACC AGACCAACCG CCTGCAGGAG GCTCTGAACC TCTTCAAGAG 
     3781 CATCTGGAAC AACAGATGGC TGCGCACCAT CTCTGTGATC CTGTTCCTCA ACAAGCAAGA 
     3841 TCTGCTCGCT GAGAAAGTCC TTGCTGGGAA ATCGAAGATT GAGGACTACT TTCCAGAATT 
     3901 TGCTCGCTAC ACTACTCCTG AGGATGCTAC TCCCGAGCCC GGAGAGGACC CACGCGTGAC 
     3961 CCGGGCCAAG TACTTCATTC GAGATGAGTT TCTGAGGATC AGCACTGCCA GTGGAGATGG 
     4021 GCGTCACTAC TGCTACCCTC ATTTCACCTG CGCTGTGGAC ACTGAGAACA TCCGCCGTGT 
     4081 GTTCAACGAC TGCCGTGACA TCATTCAGCG CATGCACCTT CGTCAGTACG AGCTGCTCAA 
     4141 GCTTAACTAG CTAGCGGACC GACGCGTACG CGGCCGCTCG AGCAGAAACT CATCTCAGAA 
     4201 GAGGATCTGG CAGCAAATGA TATCCTGGAT TACAAGGATG ACGACGATAA GGTTTAAACG 
     4261 GCCGGCCGCG GTCATAGCTG TTTCCTGAAC AGATCCCGGG TGGCATCCCT GTGACCCCTC 
     4321 CCCAGTGCCT CTCCTGGCCC TGGAAGTTGC CACTCCAGTG CCCACCAGCC TTGTCCTAAT 
     4381 AAAATTAAGT TGCATCATTT TGTCTGACTA GGTGTCCTTC TATAATATTA TGGGGTGGAG 
     4441 GGGGGTGGTA TGGAGCAAGG GGCAAGTTGG GAAGACAACC TGTAGGGCCT GCGGGGTCTA 
     4501 TTGGGAACCA AGCTGGAGTG CAGTGGCACA ATCTTGGCTC ACTGCAATCT CCGCCTCCTG 
     4561 GGTTCAAGCG ATTCTCCTGC CTCAGCCTCC CGAGTTGTTG GGATTCCAGG CATGCATGAC 
     4621 CAGGCTCAGC TAATTTTTGT TTTTTTGGTA GAGACGGGGT TTCACCATAT TGGCCAGGCT 
     4681 GGTCTCCAAC TCCTAATCTC AGGTGATCTA CCCACCTTGG CCTCCCAAAT TGCTGGGATT 
     4741 ACAGGCGTGA ACCACTGCTC CCTTCCCTGT CCTTCTGATT TTAAAATAAC TATACCAGCA 
     4801 GGAGGACGTC CAGACACAGC ATAGGCTACC TGGCCATGCC CAACCGGTGG GACATTTGAG 
     4861 TTGCTTGCTT GGCACTGTCC TCTCATGCGT TGGGTCCACT CAGTAGATGC CTGTTGAATT 
     4921 GGGTACGCGG CCAGCGGCGA GCGGTATCAG CTCACTCAAA GGCGGTAATA CGGTTATCCA 
     4981 CAGAATCAGG GGATAACGCA GGAAAGAACA TGTGAGCAAA AGGCCAGCAA AAGGCCAGGA 
     5041 ACCGTAAAAA GGCCGCGTTG CTGGCGTTTT TCCATAGGCT CCGCCCCCCT GACGAGCATC 
     5101 ACAAAAATCG ACGCTCAAGT CAGAGGTGGC GAAACCCGAC AGGACTATAA AGATACCAGG 
     5161 CGTTTCCCCC TGGAAGCTCC CTCGTGCGCT CTCCTGTTCC GACCCTGCCG CTTACCGGAT 
     5221 ACCTGTCCGC CTTTCTCCCT TCGGGAAGCG TGGCGCTTTC TCATAGCTCA CGCTGTAGGT 
     5281 ATCTCAGTTC GGTGTAGGTC GTTCGCTCCA AGCTGGGCTG TGTGCACGAA CCCCCCGTTC 
     5341 AGCCCGACCG CTGCGCCTTA TCCGGTAACT ATCGTCTTGA GTCCAACCCG GTAAGACACG 
     5401 ACTTATCGCC ACTGGCAGCA GCCACTGGTA ACAGGATTAG CAGAGCGAGG TATGTAGGCG 
     5461 GTGCTACAGA GTTCTTGAAG TGGTGGCCTA ACTACGGCTA CACTAGAAGA ACAGTATTTG 
     5521 GTATCTGCGC TCTGCTGAAG CCAGTTACCT TCGGAAAAAG AGTTGGTAGC TCTTGATCCG 
     5581 GCAAACAAAC CACCGCTGGT AGCGGTGGTT TTTTTGTTTG CAAGCAGCAG ATTACGCGCA 
     5641 GAAAAAAAGG ATCTCAAGAA GATCCTTTGA TCTTTTCTAC GGGGTCTGAC GCTCAGTGGA 
     5701 ACGAAAACTC ACGTTAAGGG ATTTTGGTCA TGAGATTATC AAAAAGGATC TTCACCTAGA 
     5761 TCCTTTTAAA TTAAAAATGA AGTTTTAAAT CAATCTAAAG TATATATGAG TAACCTGAGG 
     5821 CTATGGCAGG GCCTGCCGCC CCGACGTTGG CTGCGAGCCC TGGGCCTTCA CCCGAACTTG 
     5881 GGGGGTGGGG TGGGGAAAAG GAAGAAACGC GGGCGTATTG GCCCCAATGG GGTCTCGGTG 
     5941 GGGTATCGAC AGAGTGCCAG CCCTGGGACC GAACCCCGCG TTTATGAACA AACGACCCAA 
     6001 CACCGTGCGT TTTATTCTGT CTTTTTATTG CCGTCATAGC GCGGGTTCCT TCCGGTATTG 
     6061 TCTCCTTCCG TGTTTCAGTT AGCCTCCCCC TAGGGTGGGC GAAGAACTCC AGCATGAGAT 
     6121 CCCCGCGCTG GAGGATCATC CAGCCGGCGT CCCGGAAAAC GATTCCGAAG CCCAACCTTT 
     6181 CATAGAAGGC GGCGGTGGAA TCGAAATCTC GTGATGGCAG GTTGGGCGTC GCTTGGTCGG 
     6241 TCATTTCGAA CCCCAGAGTC CCGCTCAGAA GAACTCGTCA AGAAGGCGAT AGAAGGCGAT 
     6301 GCGCTGCGAA TCGGGAGCGG CGATACCGTA AAGCACGAGG AAGCGGTCAG CCCATTCGCC 
     6361 GCCAAGCTCT TCAGCAATAT CACGGGTAGC CAACGCTATG TCCTGATAGC GATCCGCCAC 
     6421 ACCCAGCCGG CCACAGTCGA TGAATCCAGA AAAGCGGCCA TTTTCCACCA TGATATTCGG 
     6481 CAAGCAGGCA TCGCCATGGG TCACGACGAG ATCCTCGCCG TCGGGCATGC TCGCCTTGAG 
     6541 CCTGGCGAAC AGTTCGGCTG GCGCGAGCCC CTGATGCTCT TCGTCCAGAT CATCCTGATC 
     6601 GACAAGACCG GCTTCCATCC GAGTACGTGC TCGCTCGATG CGATGTTTCG CTTGGTGGTC 
     6661 GAATGGGCAG GTAGCCGGAT CAAGCGTATG CAGCCGCCGC ATTGCATCAG CCATGATGGA 
     6721 TACTTTCTCG GCAGGAGCAA GGTGAGATGA CAGGAGATCC TGCCCCGGCA CTTCGCCCAA 
     6781 TAGCAGCCAG TCCCTTCCCG CTTCAGTGAC AACGTCGAGC ACAGCTGCGC AAGGAACGCC 
     6841 CGTCGTGGCC AGCCACGATA GCCGCGCTGC CTCGTCTTGC AGTTCATTCA GGGCACCGGA 
     6901 CAGGTCGGTC TTGACAAAAA GAACCGGGCG CCCCTGCGCT GACAGCCGGA ACACGGCGGC 
     6961 ATCAGAGCAG CCGATTGTCT GTTGTGCCCA GTCATAGCCG AATAGCCTCT CCACCCAAGC 
     7021 GGCCGGAGAA CCTGCGTGCA ATCCATCTTG TTCAATCATG CGAAACGATC CTCATCCTGT 
     7081 CTCTTGATCG ATCTTTGCAA AAGCCTAGGC CTCCAAAAAA GCCTCCTCAC TACTTCTGGA 
     7141 ATAGCTCAGA GGCCGAGGCG GCCTCGGCCT CTGCATAAAT AAAAAAAATT AGTCAGCCAT 
     7201 GGGGCGGAGA ATGGGCGGAA CTGGGCGGAG TTAGGGGCGG GATGGGCGGA GTTAGGGGCG 
     7261 GGACTATGGT TGCTGACTAA TTGAGATGCA TGCTTTGCAT ACTTCTGCCT GCTGGGGAGC 
     7321 CTGGGGACTT TCCACACCTG GTTGCTGACT AATTGAGATG CATGCTTTGC ATACTTCTGC 
     7381 CTGCTGGGGA GCCTGGGGAC TTTCCACACC CTAACTGACA CACATTCCAC AGCTGGTTCT 
     7441 TTCCGCCTCA GGACTCTTCC TTTTTCAATA TTATTGAAGC ATTTATCAGG GTTATTGTCT 
     7501 CATGAGCGGA TACATATTTG AATGTATTTA GAAAAATAAA CAAATAGGGG TTCCGCGCAC 
     7561 ATTTCCCCGA AAAGTGCCAC CTGACGCGCC CTGTAGCGGC GCATTAAGCG CGGCGGGTGT 
     7621 GGTGGTTACG CGCAGCGTGA CCGCTACACT TGCCAGCGCC CTAGCGCCCG CTCCTTTCGC 
     7681 TTTCTTCCCT TCCTTTCTCG CCACGTTCGC CGGCTTTCCC CGTCAAGCTC TAAATCGGGG 
     7741 GCTCCCTTTA GGGTTCCGAT TTAGTGCTTT ACGGCACCTC GACCCCAAAA AACTTGATTA 
     7801 GGGTGATGGT TCACGTAGTG GGCCATCGCC CTGATAGACG GTTTTTCGCC CTTTGACGTT 
     7861 GGAGTCCACG TTCTTTAATA GTGGACTCTT GTTCCAAACT GGAACAACAC TCAACCCTAT 
     7921 CTCGGTCTAT TCTTTTGATT TATAAGGGAT TTTGCCGATT TCGGCCTATT GGTTAAAAAA 
     7981 TGAGCTGATT TAACAAAAAT TTAACGCGAA TTTT
//