LOCUS       RC214198                9210 bp    DNA     circular  SYN 
DEFINITION  PTPRU (Myc-DDK-tagged)-Human protein tyrosine phosphatase, receptor type, U (PTPRU), transcript variant 2
ACCESSION   NM_133177
VERSION     NM_133177.3
SOURCE      synthetic construct 
FEATURES    Location/Qualifiers 
     misc_feature    201..924 
                     /label="CMV promoter"  
     misc_feature    839..856 
                     /label="Restriction Enzyme"  
     misc_feature    953..971 
                     /label="T7 promoter"  
     misc_feature    5365..5456 
                     /label="Myc-DDK"  
     misc_feature    5486..6071 
                     /label="PolyA signal"  
     misc_feature    5543..5562 
                     /label="Restriction Enzyme"  
     misc_feature    6347..7019 
                     /label="ColE1"  
     misc_feature    7449..8243 
                     /label="Kan/Neo"  
     misc_feature    8278..8580 
                     /label="SV40 ori"  
     misc_feature    8666..9143 
                     /label="f1 ori"  
     misc_feature    1020..1028 
                     /label="Restriction Enzyme"  
     misc_feature    5340..5347 
                     /label="Restriction Enzyme"  
     misc_feature    1020..5340 
                     /label="PTPRU"  
ORIGIN                      
        1 AACAAAATAT TAACGCTTAC AATTTCCATT CGCCATTCAG GCTGCGCAAC TGTTGGGAAG 
       61 GGCGATCGGT GCGGGCCTCT TCGCTATTAC GCCAGCTGGC GAAAGGGGGA TGTGCTGCAA 
      121 GGCGATTAAG TTGGGTAACG CCAGGGTTTT CCCAGTCACG ACGTTGTAAA ACGACGGCCA 
      181 GTGCCAAGCT GATCTATACA TTGAATCAAT ATTGGCAATT AGCCATATTA GTCATTGGTT 
      241 ATATAGCATA AATCAATATT GGCTATTGGC CATTGCATAC GTTGTATCTA TATCATAATA 
      301 TGTACATTTA TATTGGCTCA TGTCCAATAT GACCGCCATG TTGACATTGA TTATTGACTA 
      361 GTTATTAATA GTAATCAATT ACGGGGTCAT TAGTTCATAG CCCATATATG GAGTTCCGCG 
      421 TTACATAACT TACGGTAAAT GGCCCGCCTG GCTGACCGCC CAACGACCCC CGCCCATTGA 
      481 CGTCAATAAT GACGTATGTT CCCATAGTAA CGCCAATAGG GACTTTCCAT TGACGTCAAT 
      541 GGGTGGAGTA TTTACGGTAA ACTGCCCACT TGGCAGTACA TCAAGTGTAT CATATGCCAA 
      601 GTCCGCCCCC TATTGACGTC AATGACGGTA AATGGCCCGC CTGGCATTAT GCCCAGTACA 
      661 TGACCTTACG GGACTTTCCT ACTTGGCAGT ACATCTACGT ATTAGTCATC GCTATTACCA 
      721 TGGTGATGCG GTTTTGGCAG TACACCAATG GGCGTGGATA GCGGTTTGAC TCACGGGGAT 
      781 TTCCAAGTCT CCACCCCATT GACGTCAATG GGAGTTTGTT TTGGCACCAA AATCAACGGG 
      841 ACTTTCCAAA ATGTCGTAAT AACCCCGCCC CGTTGACGCA AATGGGCGGT AGGCGTGTAC 
      901 GGTGGGAGGT CTATATAAGC AGAGCTCGTT TAGTGAACCG TCAGAATTTT GTAATACGAC 
      961 TCACTATAGG GCGGCCGGGA ATTCGTCGAC TGGATCCGGT ACCGAGGAGA TCTGCCGCCG 
     1021 CGATCGCCAT GGCCCGTGCC CAGGCGCTCG TGCTGGCACT CACCTTCCAG CTCTGCGCGC 
     1081 CGGAGACCGA GACTCCGGCA GCTGGCTGCA CCTTCGAGGA GGCAAGTGAC CCAGCAGTGC 
     1141 CCTGCGAGTA CAGCCAGGCC CAGTACGATG ACTTCCAGTG GGAGCAAGTG CGAATCCACC 
     1201 CTGGCACCCG GGCACCTGCG GACCTGCCCC ACGGCTCCTA CTTGATGGTC AACACTTCCC 
     1261 AGCATGCCCC AGGCCAGCGA GCCCATGTCA TCTTCCAGAG CCTGAGCGAG AATGATACCC 
     1321 ACTGTGTGCA GTTCAGCTAC TTCCTGTACA GCCGGGACGG GCACAGCCCG GGCACCCTGG 
     1381 GCGTCTACGT GCGCGTTAAT GGGGGCCCCC TGGGCAGTGC TGTGTGGAAT ATGACTGGAT 
     1441 CCCACGGCCG TCAGTGGCAC CAGGCTGAGC TGGCTGTCAG CACTTTCTGG CCCAATGAAT 
     1501 ATCAGGTGCT GTTTGAGGCC CTCATCTCCC CAGACCGCAG GGGCTACATG GGCCTAGATG 
     1561 ACATCCTGCT TCTCAGCTAC CCCTGCGCAA AGGCCCCACA CTTCTCCCGC CTGGGCGACG 
     1621 TGGAGGTCAA CGCGGGCCAG AACGCGTCGT TCCAGTGCAT GGCCGCGGGC AGAGCGGCCG 
     1681 AGGCCGAACG CTTCCTCTTG CAACGGCAGA GCGGGGCGCT GGTGCCGGCG GCGGGCGTGC 
     1741 GGCACATCAG CCACCGGCGC TTCCTGGCCA CTTTCCCGCT GGCTGCCGTG AGCCGCGCCG 
     1801 AGCAGGACCT GTACCGCTGT GTGTCCCAGG CCCCGCGCGG CGCGGGCGTC TCTAACTTCG 
     1861 CGGAGCTCAT CGTCAAGGAG CCCCCAACTC CCATCGCGCC CCCACAGCTG CTGCGTGCTG 
     1921 GCCCCACCTA CCTCATCATC CAGCTCAACA CCAACTCCAT CATTGGCGAC GGGCCGATCG 
     1981 TGCGCAAGGA GATTGAGTAC CGCATGGCGC GCGGGCCCTG GGCTGAGGTG CACGCCGTCA 
     2041 GCCTGCAGAC CTACAAGCTG TGGCACCTCG ACCCCGACAC AGAGTATGAG ATCAGCGTGC 
     2101 TGCTCACGCG TCCCGGAGAC GGCGGCACTG GCCGCCCTGG GCCACCCCTC ATCAGCCGCA 
     2161 CCAAATGCGC AGAGCCCATG AGGGCCCCCA AAGGCCTGGC TTTTGCTGAG ATCCAGGCCC 
     2221 GTCAGCTGAC CCTGCAGTGG GAACCACTGG GCTACAACGT GACGCGTTGC CACACCTATA 
     2281 CTGTGTCGCT GTGCTATCAC TACACCCTGG GCAGCAGCCA CAACCAGACC ATCCGAGAGT 
     2341 GTGTGAAGAC AGAGCAAGGT GTCAGCCGCT ACACCATCAA GAACCTGCTG CCCTATCGGA 
     2401 ACGTTCACGT GAGGCTTGTC CTCACTAACC CTGAGGGGCG CAAAGAGGGC AAGGAGGTCA 
     2461 CTTTCCAGAC GGATGAGGAT GTGCCCAGTG GGATTGCAGC CGAGTCCCTG ACCTTCACTC 
     2521 CACTGGAGGA CATGATCTTC CTCAAGTGGG AGGAGCCCCA GGAGCCCAAT GGTCTCATCA 
     2581 CCCAGTATGA GATCAGCTAC CAGAGCATCG AGTCATCAGA CCCGGCAGTG AACGTGCCAG 
     2641 GCCCACGACG TACCATCTCC AAGCTCCGCA ATGAGACCTA CCATGTCTTC TCCAACCTGC 
     2701 ACCCAGGCAC CACCTACCTG TTCTCCGTGC GGGCCCGCAC AGGCAAAGGC TTCGGCCAGG 
     2761 CGGCACTCAC TGAGATAACC ACTAACATCT CTGCTCCCAG CTTTGATTAT GCCGACATGC 
     2821 CGTCACCCCT GGGCGAGTCT GAGAACACCA TCACCGTGCT GCTGAGGCCG GCACAGGGCC 
     2881 GCGGTGCGCC CATCAGTGTG TACCAGGTGA TTGTGGAGGA GGAGCGGGCG CGGAGGCTGC 
     2941 GGCGGGAGCC AGGTGGACAG GACTGCTTCC CAGTGCCATT GACCTTCGAG GCGGCGCTGG 
     3001 CCCGAGGCCT GGTGCACTAC TTCGGGGCCG AACTGGCGGC CAGCAGTCTA CCTGAGGCCA 
     3061 TGCCCTTTAC CGTGGGTGAC AACCAGACCT ACCGAGGCTT CTGGAACCCA CCACTTGAGC 
     3121 CTAGGAAGGC CTATCTCATC TACTTCCAGG CAGCAAGCCA CCTGAAGGGG GAGACCCGGC 
     3181 TGAATTGCAT CCGCATTGCC AGGAAAGCTG CCTGCAAGGA AAGCAAGCGG CCCCTGGAGG 
     3241 TGTCCCAGAG ATCGGAGGAG ATGGGGCTTA TCCTGGGCAT CTGTGCAGGG GGGCTTGCTG 
     3301 TCCTCATCCT TCTCCTGGGT GCCATCATTG TCATCATCCG CAAAGGGAAG CCGGTGAACA 
     3361 TGACCAAGGC CACCGTCAAC TACCGCCAGG AGAAGACACA CATGATGAGC GCCGTGGACC 
     3421 GCAGCTTCAC AGACCAGAGC ACCCTGCAGG AGGACGAGCG GCTGGGCCTG TCCTTCATGG 
     3481 ACACCCATGG CTACAGCACC CGGGGAGACC AGCGCAGCGG TGGGGTCACT GAGGCCAGCA 
     3541 GCCTCCTGGG GGGCTCCCCG AGGCGTCCCT GTGGCCGGAA GGGCTCCCCA TACCACACGG 
     3601 GGCAGCTGCA CCCTGCGGTG CGTGTCGCAG ACCTTCTGCA GCACATCAAC CAGATGAAGA 
     3661 CGGCCGAGGG TTACGGCTTC AAGCAGGAGT ATGAGAGCTT CTTTGAAGGC TGGGACGCCA 
     3721 CAAAGAAGAA AGACAAGGTC AAGGGCAGCC GGCAGGAGCC AATGCCTGCC TATGATCGGC 
     3781 ACCGAGTGAA ACTGCACCCG ATGCTGGGAG ACCCCAATGC CGACTACATT AATGCCAACT 
     3841 ACATAGATAT TCGGATAAAC CGAGAAGGTT ACCACAGGTC AAACCACTTC ATAGCCACTC 
     3901 AAGGGCCGAA GCCTGAGATG GTCTATGACT TCTGGCGTAT GGTGTGGCAG GAGCACTGTT 
     3961 CCAGCATCGT CATGATCACC AAGCTGGTCG AGGTGGGCAG GGTGAAATGC TCACGGTACT 
     4021 GGCCGGAGGA CTCAGACACC TACGGGGACA TCAAGATTAT GCTGGTGAAG ACAGAGACCC 
     4081 TGGCTGAGTA TGTCGTGCGC ACTTTTGCCC TGGAGCGGAG AGGCTACTCT GCCCGGCACG 
     4141 AGGTCCGCCA GTTCCACTTC ACAGCGTGGC CAGAGCATGG CGTCCCCTAC CATGCCACGG 
     4201 GGCTGCTGGC TTTCATCCGG CGCGTGAAGG CCTCCACCCC ACCTGATGCC GGGCCCATTG 
     4261 TCATCCACTG CAGCGCGGGC ACCGGCCGCA CAGGTTGCTA TATCGTCCTG GATGTGATGC 
     4321 TGGACATGGC AGAGTGTGAG GGCGTCGTGG ACATTTACAA CTGTGTGAAG ACTCTCTGCT 
     4381 CCCGGCGTGT CAACATGATC CAGACTGAGG AGCAGTACAT CTTCATTCAT GATGCAATCC 
     4441 TGGAGGCCTG CCTGTGTGGG GAGACCACCA TCCCTGTCAG TGAGTTCAAG GCCACCTACA 
     4501 AGGAGATGAT CCGCATTGAT CCTCAGAGTA ATTCCTCCCA GCTGCGGGAA GAGTTCCAGA 
     4561 CGCTGAACTC GGTCACCCCG CCGCTGGACG TGGAGGAGTG CAGCATCGCC CTGTTGCCCC 
     4621 GGAACCGCGA CAAGAACCGC AGCATGGACG TCCTGCCGCC CGACCGCTGC CTGCCCTTCC 
     4681 TCATCTCCAC TGATGGGGAC TCCAACAACT ACATTAATGC AGCCCTGACT GACAGCTACA 
     4741 CACGGAGTGC GGCCTTCATC GTGACCCTGC ACCCGCTGCA GAGCACCACG CCCGACTTCT 
     4801 GGCGGCTGGT CTACGATTAC GGGTGCACCT CCATCGTCAT GCTCAACCAG CTGAACCAGT 
     4861 CCAACTCCGC CTGGCCCTGC CTGCAGTACT GGCCAGAGCC AGGCCGGCAG CAATATGGCC 
     4921 TCATGGAGGT GGAGTTTATG TCGGGCACAG CTGATGAAGA CTTAGTGGCT CGAGTCTTCC 
     4981 GGGTGCAGAA CATCTCTCGG GAGGGGCACC TGCTGGTGCG GCACTTCCAG TTCCTGCGCT 
     5041 GGTCTGCATA CCGGGACACA CCTGACTCCA AGAAGGCCTT CTTGCACCTG CTGGCTGAGG 
     5101 TGGACAAGTG GCAGGCCGAG AGTGGGGATG GGCGCACCAT CGTGCACTGC CTAAACGGGG 
     5161 GAGGACGCAG CGGCACCTTC TGCGCCTGCG CCACGGTCCT GGAGATGATC CGCTGCCACA 
     5221 ACTTGGTGGA CGTTTTCTTT GCTGCCAAAA CCCTCCGGAA CTACAAACCC AACATGGTGG 
     5281 AGACCATGGA TCAGTACCAC TTTTGCTACG ATGTGGCCCT GGAGTACTTG GAGGGGCTGG 
     5341 AGTCAAGAAG CGGACCGACG CGTACGCGGC CGCTCGAGCA GAAACTCATC TCAGAAGAGG 
     5401 ATCTGGCAGC AAATGATATC CTGGATTACA AGGATGACGA CGATAAGGTT TAAACGGCCG 
     5461 GCCGCGGTCA TAGCTGTTTC CTGAACAGAT CCCGGGTGGC ATCCCTGTGA CCCCTCCCCA 
     5521 GTGCCTCTCC TGGCCCTGGA AGTTGCCACT CCAGTGCCCA CCAGCCTTGT CCTAATAAAA 
     5581 TTAAGTTGCA TCATTTTGTC TGACTAGGTG TCCTTCTATA ATATTATGGG GTGGAGGGGG 
     5641 GTGGTATGGA GCAAGGGGCA AGTTGGGAAG ACAACCTGTA GGGCCTGCGG GGTCTATTGG 
     5701 GAACCAAGCT GGAGTGCAGT GGCACAATCT TGGCTCACTG CAATCTCCGC CTCCTGGGTT 
     5761 CAAGCGATTC TCCTGCCTCA GCCTCCCGAG TTGTTGGGAT TCCAGGCATG CATGACCAGG 
     5821 CTCAGCTAAT TTTTGTTTTT TTGGTAGAGA CGGGGTTTCA CCATATTGGC CAGGCTGGTC 
     5881 TCCAACTCCT AATCTCAGGT GATCTACCCA CCTTGGCCTC CCAAATTGCT GGGATTACAG 
     5941 GCGTGAACCA CTGCTCCCTT CCCTGTCCTT CTGATTTTAA AATAACTATA CCAGCAGGAG 
     6001 GACGTCCAGA CACAGCATAG GCTACCTGGC CATGCCCAAC CGGTGGGACA TTTGAGTTGC 
     6061 TTGCTTGGCA CTGTCCTCTC ATGCGTTGGG TCCACTCAGT AGATGCCTGT TGAATTGGGT 
     6121 ACGCGGCCAG CGGCGAGCGG TATCAGCTCA CTCAAAGGCG GTAATACGGT TATCCACAGA 
     6181 ATCAGGGGAT AACGCAGGAA AGAACATGTG AGCAAAAGGC CAGCAAAAGG CCAGGAACCG 
     6241 TAAAAAGGCC GCGTTGCTGG CGTTTTTCCA TAGGCTCCGC CCCCCTGACG AGCATCACAA 
     6301 AAATCGACGC TCAAGTCAGA GGTGGCGAAA CCCGACAGGA CTATAAAGAT ACCAGGCGTT 
     6361 TCCCCCTGGA AGCTCCCTCG TGCGCTCTCC TGTTCCGACC CTGCCGCTTA CCGGATACCT 
     6421 GTCCGCCTTT CTCCCTTCGG GAAGCGTGGC GCTTTCTCAT AGCTCACGCT GTAGGTATCT 
     6481 CAGTTCGGTG TAGGTCGTTC GCTCCAAGCT GGGCTGTGTG CACGAACCCC CCGTTCAGCC 
     6541 CGACCGCTGC GCCTTATCCG GTAACTATCG TCTTGAGTCC AACCCGGTAA GACACGACTT 
     6601 ATCGCCACTG GCAGCAGCCA CTGGTAACAG GATTAGCAGA GCGAGGTATG TAGGCGGTGC 
     6661 TACAGAGTTC TTGAAGTGGT GGCCTAACTA CGGCTACACT AGAAGAACAG TATTTGGTAT 
     6721 CTGCGCTCTG CTGAAGCCAG TTACCTTCGG AAAAAGAGTT GGTAGCTCTT GATCCGGCAA 
     6781 ACAAACCACC GCTGGTAGCG GTGGTTTTTT TGTTTGCAAG CAGCAGATTA CGCGCAGAAA 
     6841 AAAAGGATCT CAAGAAGATC CTTTGATCTT TTCTACGGGG TCTGACGCTC AGTGGAACGA 
     6901 AAACTCACGT TAAGGGATTT TGGTCATGAG ATTATCAAAA AGGATCTTCA CCTAGATCCT 
     6961 TTTAAATTAA AAATGAAGTT TTAAATCAAT CTAAAGTATA TATGAGTAAC CTGAGGCTAT 
     7021 GGCAGGGCCT GCCGCCCCGA CGTTGGCTGC GAGCCCTGGG CCTTCACCCG AACTTGGGGG 
     7081 GTGGGGTGGG GAAAAGGAAG AAACGCGGGC GTATTGGCCC CAATGGGGTC TCGGTGGGGT 
     7141 ATCGACAGAG TGCCAGCCCT GGGACCGAAC CCCGCGTTTA TGAACAAACG ACCCAACACC 
     7201 GTGCGTTTTA TTCTGTCTTT TTATTGCCGT CATAGCGCGG GTTCCTTCCG GTATTGTCTC 
     7261 CTTCCGTGTT TCAGTTAGCC TCCCCCTAGG GTGGGCGAAG AACTCCAGCA TGAGATCCCC 
     7321 GCGCTGGAGG ATCATCCAGC CGGCGTCCCG GAAAACGATT CCGAAGCCCA ACCTTTCATA 
     7381 GAAGGCGGCG GTGGAATCGA AATCTCGTGA TGGCAGGTTG GGCGTCGCTT GGTCGGTCAT 
     7441 TTCGAACCCC AGAGTCCCGC TCAGAAGAAC TCGTCAAGAA GGCGATAGAA GGCGATGCGC 
     7501 TGCGAATCGG GAGCGGCGAT ACCGTAAAGC ACGAGGAAGC GGTCAGCCCA TTCGCCGCCA 
     7561 AGCTCTTCAG CAATATCACG GGTAGCCAAC GCTATGTCCT GATAGCGATC CGCCACACCC 
     7621 AGCCGGCCAC AGTCGATGAA TCCAGAAAAG CGGCCATTTT CCACCATGAT ATTCGGCAAG 
     7681 CAGGCATCGC CATGGGTCAC GACGAGATCC TCGCCGTCGG GCATGCTCGC CTTGAGCCTG 
     7741 GCGAACAGTT CGGCTGGCGC GAGCCCCTGA TGCTCTTCGT CCAGATCATC CTGATCGACA 
     7801 AGACCGGCTT CCATCCGAGT ACGTGCTCGC TCGATGCGAT GTTTCGCTTG GTGGTCGAAT 
     7861 GGGCAGGTAG CCGGATCAAG CGTATGCAGC CGCCGCATTG CATCAGCCAT GATGGATACT 
     7921 TTCTCGGCAG GAGCAAGGTG AGATGACAGG AGATCCTGCC CCGGCACTTC GCCCAATAGC 
     7981 AGCCAGTCCC TTCCCGCTTC AGTGACAACG TCGAGCACAG CTGCGCAAGG AACGCCCGTC 
     8041 GTGGCCAGCC ACGATAGCCG CGCTGCCTCG TCTTGCAGTT CATTCAGGGC ACCGGACAGG 
     8101 TCGGTCTTGA CAAAAAGAAC CGGGCGCCCC TGCGCTGACA GCCGGAACAC GGCGGCATCA 
     8161 GAGCAGCCGA TTGTCTGTTG TGCCCAGTCA TAGCCGAATA GCCTCTCCAC CCAAGCGGCC 
     8221 GGAGAACCTG CGTGCAATCC ATCTTGTTCA ATCATGCGAA ACGATCCTCA TCCTGTCTCT 
     8281 TGATCGATCT TTGCAAAAGC CTAGGCCTCC AAAAAAGCCT CCTCACTACT TCTGGAATAG 
     8341 CTCAGAGGCC GAGGCGGCCT CGGCCTCTGC ATAAATAAAA AAAATTAGTC AGCCATGGGG 
     8401 CGGAGAATGG GCGGAACTGG GCGGAGTTAG GGGCGGGATG GGCGGAGTTA GGGGCGGGAC 
     8461 TATGGTTGCT GACTAATTGA GATGCATGCT TTGCATACTT CTGCCTGCTG GGGAGCCTGG 
     8521 GGACTTTCCA CACCTGGTTG CTGACTAATT GAGATGCATG CTTTGCATAC TTCTGCCTGC 
     8581 TGGGGAGCCT GGGGACTTTC CACACCCTAA CTGACACACA TTCCACAGCT GGTTCTTTCC 
     8641 GCCTCAGGAC TCTTCCTTTT TCAATATTAT TGAAGCATTT ATCAGGGTTA TTGTCTCATG 
     8701 AGCGGATACA TATTTGAATG TATTTAGAAA AATAAACAAA TAGGGGTTCC GCGCACATTT 
     8761 CCCCGAAAAG TGCCACCTGA CGCGCCCTGT AGCGGCGCAT TAAGCGCGGC GGGTGTGGTG 
     8821 GTTACGCGCA GCGTGACCGC TACACTTGCC AGCGCCCTAG CGCCCGCTCC TTTCGCTTTC 
     8881 TTCCCTTCCT TTCTCGCCAC GTTCGCCGGC TTTCCCCGTC AAGCTCTAAA TCGGGGGCTC 
     8941 CCTTTAGGGT TCCGATTTAG TGCTTTACGG CACCTCGACC CCAAAAAACT TGATTAGGGT 
     9001 GATGGTTCAC GTAGTGGGCC ATCGCCCTGA TAGACGGTTT TTCGCCCTTT GACGTTGGAG 
     9061 TCCACGTTCT TTAATAGTGG ACTCTTGTTC CAAACTGGAA CAACACTCAA CCCTATCTCG 
     9121 GTCTATTCTT TTGATTTATA AGGGATTTTG CCGATTTCGG CCTATTGGTT AAAAAATGAG 
     9181 CTGATTTAAC AAAAATTTAA CGCGAATTTT 
//