LOCUS       RC214194                8286 bp    DNA     circular  SYN 
DEFINITION  SLC12A6 (Myc-DDK-tagged)-Human solute carrier family 12 (potassium/chloride transporters), member 6 (SLC12A6), transcript variant 6
ACCESSION   NM_001042497
VERSION     NM_001042497.1
SOURCE      synthetic construct 
FEATURES    Location/Qualifiers 
     misc_feature    201..924 
                     /label="CMV promoter"  
     misc_feature    839..856 
                     /label="Restriction Enzyme"  
     misc_feature    953..971 
                     /label="T7 promoter"  
     misc_feature    4443..4534 
                     /label="Myc-DDK"  
     misc_feature    4564..5149 
                     /label="PolyA signal"  
     misc_feature    4621..4640 
                     /label="Restriction Enzyme"  
     misc_feature    5425..6097 
                     /label="ColE1"  
     misc_feature    6527..7321 
                     /label="Kan/Neo"  
     misc_feature    7356..7658 
                     /label="SV40 ori"  
     misc_feature    7744..8221 
                     /label="f1 ori"  
     misc_feature    1020..1028 
                     /label="Restriction Enzyme"  
     misc_feature    4425..4431 
                     /label="Restriction Enzyme"  
     misc_feature    1020..4425 
                     /label="SLC12A6"  
ORIGIN                      
        1 AACAAAATAT TAACGCTTAC AATTTCCATT CGCCATTCAG GCTGCGCAAC TGTTGGGAAG 
       61 GGCGATCGGT GCGGGCCTCT TCGCTATTAC GCCAGCTGGC GAAAGGGGGA TGTGCTGCAA 
      121 GGCGATTAAG TTGGGTAACG CCAGGGTTTT CCCAGTCACG ACGTTGTAAA ACGACGGCCA 
      181 GTGCCAAGCT GATCTATACA TTGAATCAAT ATTGGCAATT AGCCATATTA GTCATTGGTT 
      241 ATATAGCATA AATCAATATT GGCTATTGGC CATTGCATAC GTTGTATCTA TATCATAATA 
      301 TGTACATTTA TATTGGCTCA TGTCCAATAT GACCGCCATG TTGACATTGA TTATTGACTA 
      361 GTTATTAATA GTAATCAATT ACGGGGTCAT TAGTTCATAG CCCATATATG GAGTTCCGCG 
      421 TTACATAACT TACGGTAAAT GGCCCGCCTG GCTGACCGCC CAACGACCCC CGCCCATTGA 
      481 CGTCAATAAT GACGTATGTT CCCATAGTAA CGCCAATAGG GACTTTCCAT TGACGTCAAT 
      541 GGGTGGAGTA TTTACGGTAA ACTGCCCACT TGGCAGTACA TCAAGTGTAT CATATGCCAA 
      601 GTCCGCCCCC TATTGACGTC AATGACGGTA AATGGCCCGC CTGGCATTAT GCCCAGTACA 
      661 TGACCTTACG GGACTTTCCT ACTTGGCAGT ACATCTACGT ATTAGTCATC GCTATTACCA 
      721 TGGTGATGCG GTTTTGGCAG TACACCAATG GGCGTGGATA GCGGTTTGAC TCACGGGGAT 
      781 TTCCAAGTCT CCACCCCATT GACGTCAATG GGAGTTTGTT TTGGCACCAA AATCAACGGG 
      841 ACTTTCCAAA ATGTCGTAAT AACCCCGCCC CGTTGACGCA AATGGGCGGT AGGCGTGTAC 
      901 GGTGGGAGGT CTATATAAGC AGAGCTCGTT TAGTGAACCG TCAGAATTTT GTAATACGAC 
      961 TCACTATAGG GCGGCCGGGA ATTCGTCGAC TGGATCCGGT ACCGAGGAGA TCTGCCGCCG 
     1021 CGATCGCCAT GCATCCTCCA GAAACCACCA CCAAGATGGC TTCAGTTCGG TTCATGGTGA 
     1081 CACCGACAAA GATCGATGAC ATTCCAGGTT TGTCAGACAC CAGTCCGGAC CTCAGCTCTC 
     1141 GATCTAGTTC CCGAGTAAGA TTTAGCTCCC GGGAAAGCGT GCCTGAAACA AGCCGGAGTG 
     1201 AGCCTATGAG TGAGATGTCT GGGGCCACCA CTTCGCTGGC AACTGTTGCA CTGGATCCAC 
     1261 CCAGTGACCG GACTTCTCAC CCCCAGGATG TCATCGAGGA CGACGGACAT AAGAAAGCTC 
     1321 GAAATGCTTA TCTCAATAAT TCCAATTATG AAGAAGGAGA TGAATATTTT GATAAAAATT 
     1381 TGGCACTCTT TGAGGAAGAA ATGGACACCA GACCGAAGGT GTCTTCCCTC CTCAACCGCA 
     1441 TGGCCAATTA CACTAATCTG ACTCAAGGAG CAAAGGAACA TGAAGAGGCA GAAAACATCA 
     1501 CTGAAGGGAA AAAGAAGCCC ACCAAGACCC CCCAAATGGG TACCTTCATG GGTGTCTACC 
     1561 TCCCATGTCT ACAAAATATT TTTGGAGTGA TCCTTTTTTT ACGCCTTACA TGGGTGGTGG 
     1621 GCACAGCTGG AGTTCTTCAG GCTTTTGCAA TTGTCCTTAT CTGCTGCTGC TGTACAATGT 
     1681 TGACTGCTAT CTCCATGAGT GCCATTGCCA CTAATGGAGT GGTGCCAGCT GGGGGCTCAT 
     1741 ACTTTATGAT TTCCCGGGCA CTGGGCCCAG AGTTTGGTGG GGCTGTTGGC CTCTGCTTTT 
     1801 ATCTTGGTAC CACATTTGCA GCAGCCATGT ACATCCTTGG TGCCATTGAA ATCTTTCTGG 
     1861 TCTATATCGT CCCCCGAGCT GCCATCTTTC ACAGTGATGA CGCACTCAAG GAATCAGCAG 
     1921 CCATGCTAAA TAACATGCGT GTCTACGGCA CAGCTTTCTT GGTCCTTATG GTATTAGTGG 
     1981 TATTTATCGG CGTACGCTAT GTGAACAAGT TTGCCTCACT TTTCCTGGCC TGTGTCATTG 
     2041 TGTCCATCTT GGCCATCTAT GCTGGAGCCA TCAAGTCTTC TTTTGCTCCT CCACACTTCC 
     2101 CGGTCTGCAT GCTGGGTAAC CGCACCCTTT CATCAAGACA CATTGACGTT TGCTCTAAGA 
     2161 CCAAGGAAAT TAACAACATG ACAGTCCCAT CAAAGTTATG GGGATTCTTC TGTAACTCGA 
     2221 GTCAATTTTT CAATGCCACC TGTGATGAAT ACTTTGTTCA CAATAACGTC ACTTCAATCC 
     2281 AGGGCATTCC TGGATTGGCT AGTGGTATAA TTACAGAGAA TCTTTGGAGT AATTACCTAC 
     2341 CCAAGGGAGA GATCATCGAA AAGCCTTCAG CCAAATCTTC TGATGTCTTA GGCAGCTTAA 
     2401 ACCATGAATA TGTTCTTGTT GACATCACCA CCTCCTTCAC GCTTCTGGTG GGAATCTTCT 
     2461 TTCCCTCTGT TACAGGTATC ATGGCTGGAT CAAACAGATC TGGAGATCTG AAAGATGCTC 
     2521 AGAAGTCTAT TCCGATTGGT ACTATCCTTG CCATCCTGAC CACCTCCTTT GTTTATTTAA 
     2581 GCAATGTTGT CCTTTTTGGT GCATGTATTG AAGGGGTTGT TCTCAGAGAC AAGTTCGGTG 
     2641 ATGCTGTGAA AGGTAATTTG GTGGTAGGCA CCTTATCTTG GCCATCCCCA TGGGTGATTG 
     2701 TTATTGGCTC CTTCTTTTCA ACATGTGGGG CTGGACTTCA GAGCCTCACA GGTGCACCGA 
     2761 GGCTGCTACA AGCTATTGCC AAGGATAACA TCATACCGTT TCTGAGGGTT TTTGGCCACA 
     2821 GCAAAGCCAA TGGGGAACCT ACCTGGGCTT TACTTCTAAC TGCTGCCATT GCAGAGCTTG 
     2881 GAATACTCAT TGCCTCCCTG GATCTTGTGG CCCCAATTCT TTCCATGTTT TTTCTCATGT 
     2941 GTTACCTCTT TGTAAACTTG GCATGTGCCT TGCAAACATT ACTTCGAACA CCCAACTGGA 
     3001 GACCCCGATT CCGCTACTAC CATTGGGCCC TTTCTTTCAT GGGAATGAGT ATCTGTCTGG 
     3061 CTCTGATGTT CATTTCTTCC TGGTATTATG CCATTGTAGC CATGGTAATA GCTGGTATGA 
     3121 TCTACAAGTA CATTGAATAC CAAGGAGCTG AGAAAGAATG GGGTGATGGT ATCCGTGGGC 
     3181 TGTCCCTCAG TGCAGCCCGG TTTGCTTTGC TTCGATTGGA GGAAGGACCT CCACACACTA 
     3241 AAAACTGGAG GCCTCAGTTG CTTGTATTAC TGAAACTAGA TGAAGACTTA CATGTCAAGC 
     3301 ATCCTCGCCT CCTCACCTTT GCCTCACAGC TCAAAGCAGG AAAAGGTCTC ACTATTGTGG 
     3361 GCTCTGTCAT CGTGGGGAAC TTCCTAGAGA ACTACGGTGA AGCTTTAGCT GCTGAGCAGA 
     3421 CCATAAAGCA CCTAATGGAG GCAGAGAAGG TAAAAGGATT CTGCCAGCTG GTGGTGGCCG 
     3481 CCAAGCTGAG AGAGGGCATT TCCCACCTCA TCCAGTCATG TGGCCTTGGG GGCATGAAGC 
     3541 ACAACACGGT GGTGATGGGC TGGCCTAATG GCTGGCGTCA AAGCGAAGAT GCCCGCGCTT 
     3601 GGAAGACTTT TATTGGCACA GTTCGAGTGA CAACTGCTGC CCATCTTGCA CTGCTGGTGG 
     3661 CTAAAAACAT CTCCTTCTTT CCCAGCAATG TGGAGCAATT TTCTGAGGGC AACATTGATG 
     3721 TGTGGTGGAT TGTGCATGAT GGGGGGATGC TTATGCTACT ACCATTCCTA CTGAAACAGC 
     3781 ACAAGGTGTG GCGAAAGTGC AGCATACGGA TCTTCACAGT AGCCCAATTA GAAGACAACA 
     3841 GTATCCAAAT GAAGAAGGAC CTAGCCACCT TCCTATATCA CTTACGCATT GAGGCGGAGG 
     3901 TAGAAGTGGT GGAGATGCAT GACAGTGATA TATCAGCATA TACTTACGAG CGCACTTTGA 
     3961 TGATGGAACA AAGGTCCCAG ATGCTCCGGC ACATGCGGCT ATCCAAAACA GAGCGAGACA 
     4021 GAGAGGCACA ATTGGTGAAA GACCGAAACT CAATGCTACG ATTGACCAGC ATTGGCTCTG 
     4081 ATGAGGACGA AGAGACAGAA ACCTATCAGG AGAAGGTGCA CATGACTTGG ACAAAAGACA 
     4141 AGTACATGGC ATCCCGGGGA CAAAAAGCGA AGTCAATGGA AGGATTCCAG GACCTGCTTA 
     4201 ACATGCGTCC GGACCAGTCC AATGTGAGGC GGATGCATAC AGCAGTGAAA CTCAACGAGG 
     4261 TTATAGTTAA CAAGTCCCAT GAAGCAAAGC TGGTTTTATT GAATATGCCA GGGCCACCCC 
     4321 GAAACCCTGA GGGTGATGAA AACTACATGG AGTTCCTAGA GGTGCTTACC GAGGGACTAG 
     4381 AGCGAGTCCT ACTTGTCCGG GGTGGTGGCA GTGAAGTGAT CACCATTTAT TCAACGCGTA 
     4441 CGCGGCCGCT CGAGCAGAAA CTCATCTCAG AAGAGGATCT GGCAGCAAAT GATATCCTGG 
     4501 ATTACAAGGA TGACGACGAT AAGGTTTAAA CGGCCGGCCG CGGTCATAGC TGTTTCCTGA 
     4561 ACAGATCCCG GGTGGCATCC CTGTGACCCC TCCCCAGTGC CTCTCCTGGC CCTGGAAGTT 
     4621 GCCACTCCAG TGCCCACCAG CCTTGTCCTA ATAAAATTAA GTTGCATCAT TTTGTCTGAC 
     4681 TAGGTGTCCT TCTATAATAT TATGGGGTGG AGGGGGGTGG TATGGAGCAA GGGGCAAGTT 
     4741 GGGAAGACAA CCTGTAGGGC CTGCGGGGTC TATTGGGAAC CAAGCTGGAG TGCAGTGGCA 
     4801 CAATCTTGGC TCACTGCAAT CTCCGCCTCC TGGGTTCAAG CGATTCTCCT GCCTCAGCCT 
     4861 CCCGAGTTGT TGGGATTCCA GGCATGCATG ACCAGGCTCA GCTAATTTTT GTTTTTTTGG 
     4921 TAGAGACGGG GTTTCACCAT ATTGGCCAGG CTGGTCTCCA ACTCCTAATC TCAGGTGATC 
     4981 TACCCACCTT GGCCTCCCAA ATTGCTGGGA TTACAGGCGT GAACCACTGC TCCCTTCCCT 
     5041 GTCCTTCTGA TTTTAAAATA ACTATACCAG CAGGAGGACG TCCAGACACA GCATAGGCTA 
     5101 CCTGGCCATG CCCAACCGGT GGGACATTTG AGTTGCTTGC TTGGCACTGT CCTCTCATGC 
     5161 GTTGGGTCCA CTCAGTAGAT GCCTGTTGAA TTGGGTACGC GGCCAGCGGC GAGCGGTATC 
     5221 AGCTCACTCA AAGGCGGTAA TACGGTTATC CACAGAATCA GGGGATAACG CAGGAAAGAA 
     5281 CATGTGAGCA AAAGGCCAGC AAAAGGCCAG GAACCGTAAA AAGGCCGCGT TGCTGGCGTT 
     5341 TTTCCATAGG CTCCGCCCCC CTGACGAGCA TCACAAAAAT CGACGCTCAA GTCAGAGGTG 
     5401 GCGAAACCCG ACAGGACTAT AAAGATACCA GGCGTTTCCC CCTGGAAGCT CCCTCGTGCG 
     5461 CTCTCCTGTT CCGACCCTGC CGCTTACCGG ATACCTGTCC GCCTTTCTCC CTTCGGGAAG 
     5521 CGTGGCGCTT TCTCATAGCT CACGCTGTAG GTATCTCAGT TCGGTGTAGG TCGTTCGCTC 
     5581 CAAGCTGGGC TGTGTGCACG AACCCCCCGT TCAGCCCGAC CGCTGCGCCT TATCCGGTAA 
     5641 CTATCGTCTT GAGTCCAACC CGGTAAGACA CGACTTATCG CCACTGGCAG CAGCCACTGG 
     5701 TAACAGGATT AGCAGAGCGA GGTATGTAGG CGGTGCTACA GAGTTCTTGA AGTGGTGGCC 
     5761 TAACTACGGC TACACTAGAA GAACAGTATT TGGTATCTGC GCTCTGCTGA AGCCAGTTAC 
     5821 CTTCGGAAAA AGAGTTGGTA GCTCTTGATC CGGCAAACAA ACCACCGCTG GTAGCGGTGG 
     5881 TTTTTTTGTT TGCAAGCAGC AGATTACGCG CAGAAAAAAA GGATCTCAAG AAGATCCTTT 
     5941 GATCTTTTCT ACGGGGTCTG ACGCTCAGTG GAACGAAAAC TCACGTTAAG GGATTTTGGT 
     6001 CATGAGATTA TCAAAAAGGA TCTTCACCTA GATCCTTTTA AATTAAAAAT GAAGTTTTAA 
     6061 ATCAATCTAA AGTATATATG AGTAACCTGA GGCTATGGCA GGGCCTGCCG CCCCGACGTT 
     6121 GGCTGCGAGC CCTGGGCCTT CACCCGAACT TGGGGGGTGG GGTGGGGAAA AGGAAGAAAC 
     6181 GCGGGCGTAT TGGCCCCAAT GGGGTCTCGG TGGGGTATCG ACAGAGTGCC AGCCCTGGGA 
     6241 CCGAACCCCG CGTTTATGAA CAAACGACCC AACACCGTGC GTTTTATTCT GTCTTTTTAT 
     6301 TGCCGTCATA GCGCGGGTTC CTTCCGGTAT TGTCTCCTTC CGTGTTTCAG TTAGCCTCCC 
     6361 CCTAGGGTGG GCGAAGAACT CCAGCATGAG ATCCCCGCGC TGGAGGATCA TCCAGCCGGC 
     6421 GTCCCGGAAA ACGATTCCGA AGCCCAACCT TTCATAGAAG GCGGCGGTGG AATCGAAATC 
     6481 TCGTGATGGC AGGTTGGGCG TCGCTTGGTC GGTCATTTCG AACCCCAGAG TCCCGCTCAG 
     6541 AAGAACTCGT CAAGAAGGCG ATAGAAGGCG ATGCGCTGCG AATCGGGAGC GGCGATACCG 
     6601 TAAAGCACGA GGAAGCGGTC AGCCCATTCG CCGCCAAGCT CTTCAGCAAT ATCACGGGTA 
     6661 GCCAACGCTA TGTCCTGATA GCGATCCGCC ACACCCAGCC GGCCACAGTC GATGAATCCA 
     6721 GAAAAGCGGC CATTTTCCAC CATGATATTC GGCAAGCAGG CATCGCCATG GGTCACGACG 
     6781 AGATCCTCGC CGTCGGGCAT GCTCGCCTTG AGCCTGGCGA ACAGTTCGGC TGGCGCGAGC 
     6841 CCCTGATGCT CTTCGTCCAG ATCATCCTGA TCGACAAGAC CGGCTTCCAT CCGAGTACGT 
     6901 GCTCGCTCGA TGCGATGTTT CGCTTGGTGG TCGAATGGGC AGGTAGCCGG ATCAAGCGTA 
     6961 TGCAGCCGCC GCATTGCATC AGCCATGATG GATACTTTCT CGGCAGGAGC AAGGTGAGAT 
     7021 GACAGGAGAT CCTGCCCCGG CACTTCGCCC AATAGCAGCC AGTCCCTTCC CGCTTCAGTG 
     7081 ACAACGTCGA GCACAGCTGC GCAAGGAACG CCCGTCGTGG CCAGCCACGA TAGCCGCGCT 
     7141 GCCTCGTCTT GCAGTTCATT CAGGGCACCG GACAGGTCGG TCTTGACAAA AAGAACCGGG 
     7201 CGCCCCTGCG CTGACAGCCG GAACACGGCG GCATCAGAGC AGCCGATTGT CTGTTGTGCC 
     7261 CAGTCATAGC CGAATAGCCT CTCCACCCAA GCGGCCGGAG AACCTGCGTG CAATCCATCT 
     7321 TGTTCAATCA TGCGAAACGA TCCTCATCCT GTCTCTTGAT CGATCTTTGC AAAAGCCTAG 
     7381 GCCTCCAAAA AAGCCTCCTC ACTACTTCTG GAATAGCTCA GAGGCCGAGG CGGCCTCGGC 
     7441 CTCTGCATAA ATAAAAAAAA TTAGTCAGCC ATGGGGCGGA GAATGGGCGG AACTGGGCGG 
     7501 AGTTAGGGGC GGGATGGGCG GAGTTAGGGG CGGGACTATG GTTGCTGACT AATTGAGATG 
     7561 CATGCTTTGC ATACTTCTGC CTGCTGGGGA GCCTGGGGAC TTTCCACACC TGGTTGCTGA 
     7621 CTAATTGAGA TGCATGCTTT GCATACTTCT GCCTGCTGGG GAGCCTGGGG ACTTTCCACA 
     7681 CCCTAACTGA CACACATTCC ACAGCTGGTT CTTTCCGCCT CAGGACTCTT CCTTTTTCAA 
     7741 TATTATTGAA GCATTTATCA GGGTTATTGT CTCATGAGCG GATACATATT TGAATGTATT 
     7801 TAGAAAAATA AACAAATAGG GGTTCCGCGC ACATTTCCCC GAAAAGTGCC ACCTGACGCG 
     7861 CCCTGTAGCG GCGCATTAAG CGCGGCGGGT GTGGTGGTTA CGCGCAGCGT GACCGCTACA 
     7921 CTTGCCAGCG CCCTAGCGCC CGCTCCTTTC GCTTTCTTCC CTTCCTTTCT CGCCACGTTC 
     7981 GCCGGCTTTC CCCGTCAAGC TCTAAATCGG GGGCTCCCTT TAGGGTTCCG ATTTAGTGCT 
     8041 TTACGGCACC TCGACCCCAA AAAACTTGAT TAGGGTGATG GTTCACGTAG TGGGCCATCG 
     8101 CCCTGATAGA CGGTTTTTCG CCCTTTGACG TTGGAGTCCA CGTTCTTTAA TAGTGGACTC 
     8161 TTGTTCCAAA CTGGAACAAC ACTCAACCCT ATCTCGGTCT ATTCTTTTGA TTTATAAGGG 
     8221 ATTTTGCCGA TTTCGGCCTA TTGGTTAAAA AATGAGCTGA TTTAACAAAA ATTTAACGCG 
     8281 AATTTT
//