LOCUS       RC214038                8040 bp    DNA     circular  SYN 
DEFINITION  EMILIN2 (Myc-DDK-tagged)-Human elastin microfibril interfacer 2 (EMILIN2)
ACCESSION   NM_032048
VERSION     NM_032048.2
SOURCE      synthetic construct 
FEATURES    Location/Qualifiers 
     misc_feature    201..924 
                     /label="CMV promoter"  
     misc_feature    839..856 
                     /label="Restriction Enzyme"  
     misc_feature    953..971 
                     /label="T7 promoter"  
     misc_feature    4197..4288 
                     /label="Myc-DDK"  
     misc_feature    4318..4903 
                     /label="PolyA signal"  
     misc_feature    4375..4394 
                     /label="Restriction Enzyme"  
     misc_feature    5179..5851 
                     /label="ColE1"  
     misc_feature    6281..7075 
                     /label="Kan/Neo"  
     misc_feature    7110..7412 
                     /label="SV40 ori"  
     misc_feature    7498..7975 
                     /label="f1 ori"  
     misc_feature    1020..1028 
                     /label="Restriction Enzyme"  
     misc_feature    4179..4185 
                     /label="Restriction Enzyme"  
     misc_feature    1020..4179 
                     /label="EMILIN2"  
ORIGIN                      
        1 AACAAAATAT TAACGCTTAC AATTTCCATT CGCCATTCAG GCTGCGCAAC TGTTGGGAAG 
       61 GGCGATCGGT GCGGGCCTCT TCGCTATTAC GCCAGCTGGC GAAAGGGGGA TGTGCTGCAA 
      121 GGCGATTAAG TTGGGTAACG CCAGGGTTTT CCCAGTCACG ACGTTGTAAA ACGACGGCCA 
      181 GTGCCAAGCT GATCTATACA TTGAATCAAT ATTGGCAATT AGCCATATTA GTCATTGGTT 
      241 ATATAGCATA AATCAATATT GGCTATTGGC CATTGCATAC GTTGTATCTA TATCATAATA 
      301 TGTACATTTA TATTGGCTCA TGTCCAATAT GACCGCCATG TTGACATTGA TTATTGACTA 
      361 GTTATTAATA GTAATCAATT ACGGGGTCAT TAGTTCATAG CCCATATATG GAGTTCCGCG 
      421 TTACATAACT TACGGTAAAT GGCCCGCCTG GCTGACCGCC CAACGACCCC CGCCCATTGA 
      481 CGTCAATAAT GACGTATGTT CCCATAGTAA CGCCAATAGG GACTTTCCAT TGACGTCAAT 
      541 GGGTGGAGTA TTTACGGTAA ACTGCCCACT TGGCAGTACA TCAAGTGTAT CATATGCCAA 
      601 GTCCGCCCCC TATTGACGTC AATGACGGTA AATGGCCCGC CTGGCATTAT GCCCAGTACA 
      661 TGACCTTACG GGACTTTCCT ACTTGGCAGT ACATCTACGT ATTAGTCATC GCTATTACCA 
      721 TGGTGATGCG GTTTTGGCAG TACACCAATG GGCGTGGATA GCGGTTTGAC TCACGGGGAT 
      781 TTCCAAGTCT CCACCCCATT GACGTCAATG GGAGTTTGTT TTGGCACCAA AATCAACGGG 
      841 ACTTTCCAAA ATGTCGTAAT AACCCCGCCC CGTTGACGCA AATGGGCGGT AGGCGTGTAC 
      901 GGTGGGAGGT CTATATAAGC AGAGCTCGTT TAGTGAACCG TCAGAATTTT GTAATACGAC 
      961 TCACTATAGG GCGGCCGGGA ATTCGTCGAC TGGATCCGGT ACCGAGGAGA TCTGCCGCCG 
     1021 CGATCGCCAT GTGGCAGCCC AGACGGCCCT GGCCCCGCGT GCCCTGGCGC TGGGCGCTGG 
     1081 CGCTGCTGGC CCTGGTTGGC GCGGGGCTGT GCCACGCCGG CCCGCAGCCC GGGTATCCCG 
     1141 CGCGGCCCAG CGCCAGGAAC AAGAACTGGT GCGCCTACAT CGTGAACAAG AATGTGAGCT 
     1201 GCTCCGTGCT GGAGGGAAGT GAGAGTTTTA TTCAGGCTCA GTACAACTGT GCCTGGAACC 
     1261 AGATGCCCTG TCCGTCGGCG CTGGTGTATC GAGTGAACTT CAGACCTAGA TATGTCACTA 
     1321 GGTATAAGAC AGTGACACAG TTGGAATGGA GGTGCTGTCC TGGCTTTAGA GGGGGAGATT 
     1381 GCCAAGAAGG TCCCAAAGAC CCCGTGAAGA CCCTCCGCCC CACGCCGGCT CGGCCTCGAA 
     1441 ATAGCTTGAA GAAAGCCACA GATAATGAAC CCAGCCAATT CTCAGAGCCC AGGAAGACTT 
     1501 TGTCCCCAAC TGGTACAGCA CAACCAAGCT GGGGGGTAGA TCCAAAAGAG GGGCCTCAGG 
     1561 AACTTCAGGA AAAGAAGATA CAGGTGCTAG AGGAGAAGGT TCTTCGACTC ACAAGGACGG 
     1621 TTCTTGACCT CCAGTCTTCC CTTGCTGGAG TGAGTGAAAA TCTCAAACAT GCCACTCAGG 
     1681 ATGATGCCAG TAGAACACGG GCACCAGGGC TCAGCAGCCA GCACCCCAAG CCTGACACCA 
     1741 CTGTTAGTGG AGACACAGAA ACGGGCCAGA GTCCTGGTGT CTTCAACACT AAGGAATCTG 
     1801 GCATGAAGGA CATCAAGTCT GAATTGGCTG AAGTCAAAGA TACTCTAAAG AACAAAAGTG 
     1861 ACAAGCTGGA AGAGCTGGAT GGAAAAGTGA AGGGCTACGA AGGGCAGCTC AGACAGCTCC 
     1921 AGGAAGCAGC TCAGGGCCCG ACGGTGACCA TGACAACCAA CGAACTCTAC CAAGCCTATG 
     1981 TGGACAGTAA GATCGACGCC CTGAGAGAGG AGCTCATGGA GGGCATGGAC AGAAAGCTGG 
     2041 CTGACCTGAA AAACTCATGT GAGTACAAGC TCACTGGCCT CCAGCAGCAG TGTGATGACT 
     2101 ATGGGAGCAG CTACCTGGGA GTGATAGAGC TCATAGGGGA GAAGGAAACA AGCCTGAGAA 
     2161 AAGAAATAAA TAACCTCCGA GCCCGGCTAC AGGAGCCTTC AGCCCAGGCA AATTGCTGCG 
     2221 ACAGTGAAAA GAATGGTGAC ATTGGTCAAC AGATCAAGAC ATTGGACCAG AAAATCGAGA 
     2281 GAGTTGCTGA AGCCACCAGA ATGCTGAATG GAAGACTGGA CAATGAGTTT GACCGCCTTA 
     2341 TAGTTCCAGA GCCAGATGTG GATTTTGATG CAAAATGGAA TGAACTCGAT GCAAGGATCA 
     2401 ATGTGACGGA GAAGAACGCT GAAGAACATT GCTTTTACAT TGAGGAAACC CTTCGGGGCG 
     2461 CCATTAATGG AGAGGTGGGT GACTTGAAGC AGCTTGTTGA TCAGAAAATA CAGTCTCTGG 
     2521 AAGACCGTCT GGGGAGCGTT CTCCTACAGA TGACCAATAA CACTGGTGCA GAGCTCAGTC 
     2581 CCCCAGGGGC AGCAGCCCTG CCAGGAGTGT CAGGGTCAGG AGATGAACGG GTCATGATGG 
     2641 AATTAAACCA CCTGAAGGAC AAAGTTCAAG TTGTTGAAGA CATTTGCCTG CTGAACATCC 
     2701 AGGGAAAGCC TCATGGGATG GAAGGTGCCT TGCCAAACAG GGAAGACCGC GCAGTACGCG 
     2761 ACAGCCTGCA CCTTTTGAAA TCTCTCAACG ACACGATGCA CAGGAAGTTT CAAGAAACCG 
     2821 AACAAACCAT CCAGAAACTT CAACAGGATT TTAGTTTTCT TTATTCTCAA TTAAACCACA 
     2881 CAGAAAATGA TGTGACTCAT CTTCAAAAGG AAATGAGCAA TTGTAGAGCA GGTGAAAACG 
     2941 CTGGCATGGG TAGGTTCACT AAGGTGGGTG AGCAAGAAAG GACAGTGGAC ACCCTGCCGT 
     3001 CCCCCCAGCA CCCCGTGGCT CATTGCTGCA GTCAGCTGGA GGAGAGGTGG CAGAGGTTGC 
     3061 AGAGCCAGGT CATCTCGGAG CTGGATGCTT GTAAGGAATG CACGCAGGGG GTCCAGAGGG 
     3121 AGGTCTCCAT GGTGGAGGGC AGGGTGTCTC ATATGGAGAA AACTTGCAGC AAGCTGGACT 
     3181 CTATCTCAGG AAATCTTCAG AGGATCAAGG AGGGGCTCAA CAAGCATGTC AGCAGCCTGT 
     3241 GGAACTGTGT CAGGCAGATG AACGGAACGC TCAGGTCGCA TTCCAGAGAC ATTTCTGGCC 
     3301 TGAAGAATTC AGTCCAGCAG TTCTACAGCC ACGTCTTCCA GATTTCTACT GATTTGCAAG 
     3361 ATCTGGTCAA ATTTCAGCCA TCAGCAAAGG CACCCTCGCC CCCGCCGCCC GCAGAGGCCC 
     3421 CGAAGGAGCC GCTGCAGCCC GAGCCCGCCC CGCCGAGGCC CAGCGGCCCC GCAACCGCAG 
     3481 AGGACCCTGG GCGACGGCCC GTCCTGCCCC AGCGGCCCCC CGAGGAGAGG CCGCCCCAGC 
     3541 CGCCAGGCTC CACCGGGGTC ATCGCGGAGA CGGGCCAGGC CGGGCCCCCC GCAGGCGCAG 
     3601 GCGTGTCTGG GCGGGGTCTG CCGCGGGGCG TGGACGGCCA GACCGGGAGC GGCACCGTCC 
     3661 CCGGCGCAGA AGGCTTCGCG GGCGCACCAG GATACCCGAA GTCACCTCCT GTAGCTTCCC 
     3721 CAGGAGCTCC GGTGCCTTCT CTGGTGTCTT TTTCTGCGGG GCTCACCCAG AAGCCTTTCC 
     3781 CCAGTGATGG GGGCGTTGTC CTCTTTAACA AAGTGCTGGT GAACGACGGG GATGTTTACA 
     3841 ACCCCAGCAC CGGGGTCTTC ACGGCTCCTT ATGATGGGCG CTACCTGATC ACGGCCACCC 
     3901 TCACCCCCGA GAGAGACGCC TACGTGGAAG CAGTGCTGTC GGTCTCCAAC GCCAGCGTGG 
     3961 CCCAGCTGCA TACCGCTGGG TACAGGAGAG AGTTCCTGGA ATACCACCGC CCTCCAGGAG 
     4021 CTTTGCATAC CTGCGGGGGC CCGGGGGCAT TCCACCTCAT CGTGCACCTG AAGGCGGGAG 
     4081 ATGCAGTCAA CGTCGTGGTG ACTGGGGGCA AGCTGGCTCA CACAGACTTT GATGAAATGT 
     4141 ACTCCACATT TAGTGGGGTT TTCTTATATC CTTTCCTTTC CCACCTCACG CGTACGCGGC 
     4201 CGCTCGAGCA GAAACTCATC TCAGAAGAGG ATCTGGCAGC AAATGATATC CTGGATTACA 
     4261 AGGATGACGA CGATAAGGTT TAAACGGCCG GCCGCGGTCA TAGCTGTTTC CTGAACAGAT 
     4321 CCCGGGTGGC ATCCCTGTGA CCCCTCCCCA GTGCCTCTCC TGGCCCTGGA AGTTGCCACT 
     4381 CCAGTGCCCA CCAGCCTTGT CCTAATAAAA TTAAGTTGCA TCATTTTGTC TGACTAGGTG 
     4441 TCCTTCTATA ATATTATGGG GTGGAGGGGG GTGGTATGGA GCAAGGGGCA AGTTGGGAAG 
     4501 ACAACCTGTA GGGCCTGCGG GGTCTATTGG GAACCAAGCT GGAGTGCAGT GGCACAATCT 
     4561 TGGCTCACTG CAATCTCCGC CTCCTGGGTT CAAGCGATTC TCCTGCCTCA GCCTCCCGAG 
     4621 TTGTTGGGAT TCCAGGCATG CATGACCAGG CTCAGCTAAT TTTTGTTTTT TTGGTAGAGA 
     4681 CGGGGTTTCA CCATATTGGC CAGGCTGGTC TCCAACTCCT AATCTCAGGT GATCTACCCA 
     4741 CCTTGGCCTC CCAAATTGCT GGGATTACAG GCGTGAACCA CTGCTCCCTT CCCTGTCCTT 
     4801 CTGATTTTAA AATAACTATA CCAGCAGGAG GACGTCCAGA CACAGCATAG GCTACCTGGC 
     4861 CATGCCCAAC CGGTGGGACA TTTGAGTTGC TTGCTTGGCA CTGTCCTCTC ATGCGTTGGG 
     4921 TCCACTCAGT AGATGCCTGT TGAATTGGGT ACGCGGCCAG CGGCGAGCGG TATCAGCTCA 
     4981 CTCAAAGGCG GTAATACGGT TATCCACAGA ATCAGGGGAT AACGCAGGAA AGAACATGTG 
     5041 AGCAAAAGGC CAGCAAAAGG CCAGGAACCG TAAAAAGGCC GCGTTGCTGG CGTTTTTCCA 
     5101 TAGGCTCCGC CCCCCTGACG AGCATCACAA AAATCGACGC TCAAGTCAGA GGTGGCGAAA 
     5161 CCCGACAGGA CTATAAAGAT ACCAGGCGTT TCCCCCTGGA AGCTCCCTCG TGCGCTCTCC 
     5221 TGTTCCGACC CTGCCGCTTA CCGGATACCT GTCCGCCTTT CTCCCTTCGG GAAGCGTGGC 
     5281 GCTTTCTCAT AGCTCACGCT GTAGGTATCT CAGTTCGGTG TAGGTCGTTC GCTCCAAGCT 
     5341 GGGCTGTGTG CACGAACCCC CCGTTCAGCC CGACCGCTGC GCCTTATCCG GTAACTATCG 
     5401 TCTTGAGTCC AACCCGGTAA GACACGACTT ATCGCCACTG GCAGCAGCCA CTGGTAACAG 
     5461 GATTAGCAGA GCGAGGTATG TAGGCGGTGC TACAGAGTTC TTGAAGTGGT GGCCTAACTA 
     5521 CGGCTACACT AGAAGAACAG TATTTGGTAT CTGCGCTCTG CTGAAGCCAG TTACCTTCGG 
     5581 AAAAAGAGTT GGTAGCTCTT GATCCGGCAA ACAAACCACC GCTGGTAGCG GTGGTTTTTT 
     5641 TGTTTGCAAG CAGCAGATTA CGCGCAGAAA AAAAGGATCT CAAGAAGATC CTTTGATCTT 
     5701 TTCTACGGGG TCTGACGCTC AGTGGAACGA AAACTCACGT TAAGGGATTT TGGTCATGAG 
     5761 ATTATCAAAA AGGATCTTCA CCTAGATCCT TTTAAATTAA AAATGAAGTT TTAAATCAAT 
     5821 CTAAAGTATA TATGAGTAAC CTGAGGCTAT GGCAGGGCCT GCCGCCCCGA CGTTGGCTGC 
     5881 GAGCCCTGGG CCTTCACCCG AACTTGGGGG GTGGGGTGGG GAAAAGGAAG AAACGCGGGC 
     5941 GTATTGGCCC CAATGGGGTC TCGGTGGGGT ATCGACAGAG TGCCAGCCCT GGGACCGAAC 
     6001 CCCGCGTTTA TGAACAAACG ACCCAACACC GTGCGTTTTA TTCTGTCTTT TTATTGCCGT 
     6061 CATAGCGCGG GTTCCTTCCG GTATTGTCTC CTTCCGTGTT TCAGTTAGCC TCCCCCTAGG 
     6121 GTGGGCGAAG AACTCCAGCA TGAGATCCCC GCGCTGGAGG ATCATCCAGC CGGCGTCCCG 
     6181 GAAAACGATT CCGAAGCCCA ACCTTTCATA GAAGGCGGCG GTGGAATCGA AATCTCGTGA 
     6241 TGGCAGGTTG GGCGTCGCTT GGTCGGTCAT TTCGAACCCC AGAGTCCCGC TCAGAAGAAC 
     6301 TCGTCAAGAA GGCGATAGAA GGCGATGCGC TGCGAATCGG GAGCGGCGAT ACCGTAAAGC 
     6361 ACGAGGAAGC GGTCAGCCCA TTCGCCGCCA AGCTCTTCAG CAATATCACG GGTAGCCAAC 
     6421 GCTATGTCCT GATAGCGATC CGCCACACCC AGCCGGCCAC AGTCGATGAA TCCAGAAAAG 
     6481 CGGCCATTTT CCACCATGAT ATTCGGCAAG CAGGCATCGC CATGGGTCAC GACGAGATCC 
     6541 TCGCCGTCGG GCATGCTCGC CTTGAGCCTG GCGAACAGTT CGGCTGGCGC GAGCCCCTGA 
     6601 TGCTCTTCGT CCAGATCATC CTGATCGACA AGACCGGCTT CCATCCGAGT ACGTGCTCGC 
     6661 TCGATGCGAT GTTTCGCTTG GTGGTCGAAT GGGCAGGTAG CCGGATCAAG CGTATGCAGC 
     6721 CGCCGCATTG CATCAGCCAT GATGGATACT TTCTCGGCAG GAGCAAGGTG AGATGACAGG 
     6781 AGATCCTGCC CCGGCACTTC GCCCAATAGC AGCCAGTCCC TTCCCGCTTC AGTGACAACG 
     6841 TCGAGCACAG CTGCGCAAGG AACGCCCGTC GTGGCCAGCC ACGATAGCCG CGCTGCCTCG 
     6901 TCTTGCAGTT CATTCAGGGC ACCGGACAGG TCGGTCTTGA CAAAAAGAAC CGGGCGCCCC 
     6961 TGCGCTGACA GCCGGAACAC GGCGGCATCA GAGCAGCCGA TTGTCTGTTG TGCCCAGTCA 
     7021 TAGCCGAATA GCCTCTCCAC CCAAGCGGCC GGAGAACCTG CGTGCAATCC ATCTTGTTCA 
     7081 ATCATGCGAA ACGATCCTCA TCCTGTCTCT TGATCGATCT TTGCAAAAGC CTAGGCCTCC 
     7141 AAAAAAGCCT CCTCACTACT TCTGGAATAG CTCAGAGGCC GAGGCGGCCT CGGCCTCTGC 
     7201 ATAAATAAAA AAAATTAGTC AGCCATGGGG CGGAGAATGG GCGGAACTGG GCGGAGTTAG 
     7261 GGGCGGGATG GGCGGAGTTA GGGGCGGGAC TATGGTTGCT GACTAATTGA GATGCATGCT 
     7321 TTGCATACTT CTGCCTGCTG GGGAGCCTGG GGACTTTCCA CACCTGGTTG CTGACTAATT 
     7381 GAGATGCATG CTTTGCATAC TTCTGCCTGC TGGGGAGCCT GGGGACTTTC CACACCCTAA 
     7441 CTGACACACA TTCCACAGCT GGTTCTTTCC GCCTCAGGAC TCTTCCTTTT TCAATATTAT 
     7501 TGAAGCATTT ATCAGGGTTA TTGTCTCATG AGCGGATACA TATTTGAATG TATTTAGAAA 
     7561 AATAAACAAA TAGGGGTTCC GCGCACATTT CCCCGAAAAG TGCCACCTGA CGCGCCCTGT 
     7621 AGCGGCGCAT TAAGCGCGGC GGGTGTGGTG GTTACGCGCA GCGTGACCGC TACACTTGCC 
     7681 AGCGCCCTAG CGCCCGCTCC TTTCGCTTTC TTCCCTTCCT TTCTCGCCAC GTTCGCCGGC 
     7741 TTTCCCCGTC AAGCTCTAAA TCGGGGGCTC CCTTTAGGGT TCCGATTTAG TGCTTTACGG 
     7801 CACCTCGACC CCAAAAAACT TGATTAGGGT GATGGTTCAC GTAGTGGGCC ATCGCCCTGA 
     7861 TAGACGGTTT TTCGCCCTTT GACGTTGGAG TCCACGTTCT TTAATAGTGG ACTCTTGTTC 
     7921 CAAACTGGAA CAACACTCAA CCCTATCTCG GTCTATTCTT TTGATTTATA AGGGATTTTG 
     7981 CCGATTTCGG CCTATTGGTT AAAAAATGAG CTGATTTAAC AAAAATTTAA CGCGAATTTT 
//