LOCUS       RC214034L1                9525 bp    DNA     circular  SYN 
DEFINITION  HERC3 (Myc-DDK-tagged)-Human hect domain and RLD 3 (HERC3)
ACCESSION   NM_014606
VERSION     null
SOURCE      synthetic construct 
FEATURES    Location/Qualifiers 
     misc_feature    7816..7996 
                     /label="3'LTR"  
     misc_feature    833..1013 
                     /label="5'LTR"  
     misc_feature    8724..9383 
                     /label="CAT(Cam-r)"  
     misc_feature    2620..3234 
                     /label="CMV promoter"  
     misc_feature    8155..8723 
                     /label="ColE1"  
     misc_feature    6691..6711 
                     /label="LR50 primer"  
     misc_feature    6488..6559 
                     /label="Myc-DDK"  
     misc_feature    3252..3271 
                     /label="T7 promoter"  
     misc_feature    3221..3237 
                     /label="V2 primer"  
     misc_feature    6700..7291 
                     /label="WPRE"  
     misc_feature    3320..3328 
                     /label="Restriction Enzyme"  
     misc_feature    6470..6476 
                     /label="Restriction Enzyme"  
     misc_feature    3320..6470 
                     /label="HERC3"  
ORIGIN                      
        1 GTCGACGGAT CGGGAGATCT CCCGATCCCC TATGGTGCAC TCTCAGTACA ATCTGCTCTG 
       61 ATGCCGCATA GTTAAGCCAG TATCTGCTCC CTGCTTGTGT GTTGGAGGTC GCTGAGTAGT 
      121 GCGCGAGCAA AATTTAAGCT ACAACAAGGC AAGGCTTGAC CGACAATTGC ATGAAGAATC 
      181 TGCTTAGGGT TAGGCGTTTT GCGCTGCTTC GCGATGTACG GGCCAGATAT CGCGTTGACA 
      241 TTGATTATTG ACTAGTTATT AATAGTAATC AATTACGGGG TCATTAGTTC ATAGCCCATA 
      301 TATGGAGTTC CGCGTTACAT AACTTACGGT AAATGGCCCG CCTGGCTGAC CGCCCAACGA 
      361 CCCCCGCCCA TTGACGTCAA TAATGACGTA TGTTCCCATA GTAACGCCAA TAGGGACTTT 
      421 CCATTGACGT CAATGGGTGG AGTATTTACG GTAAACTGCC CACTTGGCAG TACATCAAGT 
      481 GTATCATATG CCAAGTACGC CCCCTATTGA CGTCAATGAC GGTAAATGGC CCGCCTGGCA 
      541 TTATGCCCAG TACATGACCT TATGGGACTT TCCTACTTGG CAGTACATCT ACGTATTAGT 
      601 CATCGCTATT ACCATGGTGA TGCGGTTTTG GCAGTACATC AATGGGCGTG GATAGCGGTT 
      661 TGACTCACGG GGATTTCCAA GTCTCCACCC CATTGACGTC AATGGGAGTT TGTTTTGGCA 
      721 CCAAAATCAA CGGGACTTTC CAAAATGTCG TAACAACTCC GCCCCATTGA CGCAAATGGG 
      781 CGGTAGGCGT GTACGGTGGG AGGTCTATAT AAGCAGCGCG TTTTGCCTGT ACTGGGTCTC 
      841 TCTGGTTAGA CCAGATCTGA GCCTGGGAGC TCTCTGGCTA ACTAGGGAAC CCACTGCTTA 
      901 AGCCTCAATA AAGCTTGCCT TGAGTGCTTC AAGTAGTGTG TGCCCGTCTG TTGTGTGACT 
      961 CTGGTAACTA GAGATCCCTC AGACCCTTTT AGTCAGTGTG GAAAATCTCT AGCAGTGGCG 
     1021 CCCGAACAGG GACTTGAAAG CGAAAGGGAA ACCAGAGGAG CTCTCTCGAC GCAGGACTCG 
     1081 GCTTGCTGAA GCGCGCACGG CAAGAGGCGA GGGGCGGCGA CTGGTGAGTA CGCCAAAAAT 
     1141 TTTGACTAGC GGAGGCTAGA AGGAGAGAGA TGGGTGCGAG AGCGTCAGTA TTAAGCGGGG 
     1201 GAGAATTAGA TCGCGATGGG AAAAAATTCG GTTAAGGCCA GGGGGAAAGA AAAAATATAA 
     1261 ATTAAAACAT ATAGTATGGG CAAGCAGGGA GCTAGAACGA TTCGCAGTTA ATCCTGGCCT 
     1321 GTTAGAAACA TCAGAAGGCT GTAGACAAAT ACTGGGACAG CTACAACCAT CCCTTCAGAC 
     1381 AGGATCAGAA GAACTTAGAT CATTATATAA TACAGTAGCA ACCCTCTATT GTGTGCATCA 
     1441 AAGGATAGAG ATAAAAGACA CCAAGGAAGC TTTAGACAAG ATAGAGGAAG AGCAAAACAA 
     1501 AAGTAAGACC ACCGCACAGC AAGCGGCCGG CCGCTGATCT TCAGACCTGG AGGAGGAGAT 
     1561 ATGAGGGACA ATTGGAGAAG TGAATTATAT AAATATAAAG TAGTAAAAAT TGAACCATTA 
     1621 GGAGTAGCAC CCACCAAGGC AAAGAGAAGA GTGGTGCAGA GAGAAAAAAG AGCAGTGGGA 
     1681 ATAGGAGCTT TGTTCCTTGG GTTCTTGGGA GCAGCAGGAA GCACTATGGG CGCAGCGTCA 
     1741 ATGACGCTGA CGGTACAGGC CAGACAATTA TTGTCTGGTA TAGTGCAGCA GCAGAACAAT 
     1801 TTGCTGAGGG CTATTGAGGC GCAACAGCAT CTGTTGCAAC TCACAGTCTG GGGCATCAAG 
     1861 CAGCTCCAGG CAAGAATCCT GGCTGTGGAA AGATACCTAA AGGATCAACA GCTCCTGGGG 
     1921 ATTTGGGGTT GCTCTGGAAA ACTCATTTGC ACCACTGCTG TGCCTTGGAA TGCTAGTTGG 
     1981 AGTAATAAAT CTCTGGAACA GATTTGGAAT CACACGACCT GGATGGAGTG GGACAGAGAA 
     2041 ATTAACAATT ACACAAGCTT AATACACTCC TTAATTGAAG AATCGCAAAA CCAGCAAGAA 
     2101 AAGAATGAAC AAGAATTATT GGAATTAGAT AAATGGGCAA GTTTGTGGAA TTGGTTTAAC 
     2161 ATAACAAATT GGCTGTGGTA TATAAAATTA TTCATAATGA TAGTAGGAGG CTTGGTAGGT 
     2221 TTAAGAATAG TTTTTGCTGT ACTTTCTATA GTGAATAGAG TTAGGCAGGG ATATTCACCA 
     2281 TTATCGTTTC AGACCCACCT CCCAACCCCG AGGGGACCCG ACAGGCCCGA AGGAATAGAA 
     2341 GAAGAAGGTG GAGAGAGAGA CAGAGACAGA TCCATTCGAT TAGTGAACGG ATCGGCACTG 
     2401 CGTGCGCCAA TTCTGCAGAC AAATGGCAGT ATTCATCCAC AATTTTAAAA GAAAAGGGGG 
     2461 GATTGGGGGG TACAGTGCAG GGGAAAGAAT AGTAGACATA ATAGCAACAG ACATACAAAC 
     2521 TAAAGAATTA CAAAAACAAA TTACAAAAAT TCAAAATTTT CGGGTTTATT ACAGGGACAG 
     2581 CAGAGATCCA GTTTGGTTAG TACCGGGCCC GCTCTAGACA TGTCCAATAT GACCGCCATG 
     2641 TTGACATTGA TTATTGACTA GTTATTAATA GTAATCAATT ACGGGGTCAT TAGTTCATAG 
     2701 CCCATATATG GAGTTCCGCG TTACATAACT TACGGTAAAT GGCCCGCCTG GCTGACCGCC 
     2761 CAACGACCCC CGCCCATTGA CGTCAATAAT GACGTATGTT CCCATAGTAA CGCCAATAGG 
     2821 GACTTTCCAT TGACGTCAAT GGGTGGAGTA TTTACGGTAA ACTGCCCACT TGGCAGTACA 
     2881 TCAAGTGTAT CATATGCCAA GTCCGCCCCC TATTGACGTC AATGACGGTA AATGGCCCGC 
     2941 CTGGCATTAT GCCCAGTACA TGACCTTACG GGACTTTCCT ACTTGGCAGT ACATCTACGT 
     3001 ATTAGTCATC GCTATTACCA TGGTGATGCG GTTTTGGCAG TACACCAATG GGCGTGGATA 
     3061 GCGGTTTGAC TCACGGGGAT TTCCAAGTCT CCACCCCATT GACGTCAATG GGAGTTTGTT 
     3121 TTGGCACCAA AATCAACGGG ACTTTCCAAA ATGTCGTAAT AACCCCGCCC CGTTGACGCA 
     3181 AATGGGCGGT AGGCGTGTAC GGTGGGAGGT CTATATAAGC AGAGCTCGTT TAGTGAACCG 
     3241 TCAGAATTTT GTAATACGAC TCACTATAGG GCGGCCGGGA ATTCGTCGAC TGGATCCGGT 
     3301 ACCGAGGAGA TCTGCCGCCG CGATCGCCAT GTTATGTTGG GGATATTGGT CTCTGGGCCA 
     3361 ACCTGGTATC AGCACCAACC TGCAGGGAAT TGTGGCTGAG CCCCAGGTGT GTGGGTTCAT 
     3421 ATCTGACAGA AGTGTCAAGG AAGTGGCCTG TGGGGGAAAC CACTCTGTGT TCCTGCTGGA 
     3481 AGATGGGGAA GTTTACACAT GTGGTTTGAA CACCAAGGGG CAACTGGGCC ATGAGAGGGA 
     3541 AGGAAACAAG CCAGAACAAA TTGGAGCTCT GGCAGATCAG CATATCATTC ATGTGGCATG 
     3601 TGGCGAGTCC CACAGTCTGG CCCTCAGTGA CCGAGGCCAG CTGTTTTCTT GGGGTGCAGG 
     3661 GAGTGATGGT CAGCTAGGAC TCATGACTAC TGAGGATTCT GTGGCAGTGC CCAGGTTAAT 
     3721 ACAAAAGCTG AACCAGCAAA CAATATTACA AGTTTCCTGT GGCAACTGGC ATTGCTTGGC 
     3781 TCTTGCGGCT GATGGCCAGT TCTTCACCTG GGGAAAGAAC AGCCATGGGC AGCTTGGCTT 
     3841 AGGGAAGGAG TTCCCCTCCC AAGCCAGCCC ACAGAGGGTG AGGTCCCTGG AGGGGATCCC 
     3901 ACTGGCTCAG GTGGCTGCCG GAGGGGCTCA CAGCTTTGCC CTGTCTCTCT CAGGAGCTGT 
     3961 TTTTGGCTGG GGGATGAATA ATGCCGGGCA GCTAGGGCTC AGTGATGAAA AAGATCGAGA 
     4021 ATCTCCATGC CATGTAAAAC TCTTACGCAC GCAAAAAGTT GTCTATATTA GTTGTGGAGA 
     4081 AGAACACACA GCAGTTCTCA CAAAGAGTGG AGGTGTGTTT ACCTTTGGCG CTGGTTCCTG 
     4141 TGGGCAACTT GGACACGACT CCATGAATGA TGAGGTTAAC CCTAGAAGAG TTCTAGAGCT 
     4201 GATGGGTAGT GAAGTAACTC AAATTGCTTG TGGCAGACAA CATACCCTAG CCTTCGTGCC 
     4261 TTCTTCTGGA CTCATCTATG CATTTGGTTG TGGAGCAAGA GGTCAATTAG GAACTGGGCA 
     4321 CACTTGTAAT GTTAAGTGCC CATCTCCTGT CAAGGGTTAC TGGGCTGCCC ACAGTGGCCA 
     4381 GCTTTCAGCC CGAGCTGATC GCTTTAAATA TCATATCGTT AAGCAGATCT TCTCTGGAGG 
     4441 AGACCAGACT TTTGTACTTT GCTCCAAATA CGAGAATTAT TCTCCTGCTG TTGACTTCAG 
     4501 GACTATGAAC CAAGCACATT ATACCAGTTT AATAAATGAT GAAACCATAG CAGTTTGGAG 
     4561 ACAAAAACTC TCAGAACACA ACAATGCAAA TACAATCAAT GGTGTTGTTC AGATATTATC 
     4621 TTCTGCAGCC TGTTGGAATG GAAGTTTTCT TGAAAAAAAA ATTGATGAAC ATTTTAAAAC 
     4681 GAGTCCCAAA ATCCCTGGGA TTGACCTGAA CTCAACTAGG GTGTTATTTG AGAAGTTAAT 
     4741 GAACTCTCAG CACTCCATGA TTCTAGAACA GATTTTGAAC AGTTTTGAAA GTTGTCTGAT 
     4801 TCCCCAGTTG TCAAGCTCAC CACCAGATGT TGAAGCCATG AGAATCTATT TAATACTACC 
     4861 TGAGTTTCCC CTACTCCAGG ATTCCAAGTA TTATATAACA TTGACTATTC CCTTGGCTAT 
     4921 GGCCATTCTT CGGCTGGATA CAAACCCCAG CAAAGTACTA GATAACTGGT GGTCTCAGGT 
     4981 ATGCCCGAAA TATTTCATGA AGCTGGTAAA CCTCTATAAA GGTGCAGTCC TTTATCTACT 
     5041 GAGGGGAAGA AAGACATTCT TAATTCCCGT ACTGTTTAAC AATTATATCA CAGCAGCTCT 
     5101 CAAACTCTTG GAGAAGTTAT ATAAGGTAAA TCTTAAAGTG AAGCATGTGG AATATGATAC 
     5161 ATTTTACATT CCTGAGATTT CCAATCTCGT GGACATTCAG GAAGACTACC TCATGTGGTT 
     5221 CTTGCATCAA GCAGGGATGA AGGCTAGACC ATCAATAATA CAGGATACTG TAACACTTTG 
     5281 TTCCTACCCT TTCATCTTTG ATGCCCAAGC CAAGACCAAA ATGTTACAGA CAGATGCTGA 
     5341 ACTACAGATG CAGGTGGCAG TCAATGGAGC CAACCTGCAG AATGTCTTCA TGCTTCTCAC 
     5401 CCTGGAGCCT CTGCTGGCCA GAAGCCCCTT CCTGGTCCTT CACGTTCGCA GGAACAACCT 
     5461 TGTTGGAGAT GCCCTAAGAG AGCTGAGCAT TCATTCTGAT ATTGATTTGA AAAAGCCTCT 
     5521 CAAAGTAATC TTTGATGGTG AAGAAGCAGT GGATGCCGGT GGTGTTACAA AGGAATTTTT 
     5581 TCTTTTGCTG TTAAAAGAAC TTTTGAATCC CATCTATGGA ATGTTTACCT ACTATCAAGA 
     5641 TTCAAATCTC TTGTGGTTTT CAGACACGTG TTTTGTAGAG CACAACTGGT TTCACTTGAT 
     5701 TGGTATAACC TGTGGACTAG CTATCTACAA CTCCACTGTG GTCGATCTCC ACTTCCCATT 
     5761 GGCTCTCTAC AAGAAGTTAC TCAATGTAAA GCCTGGCTTG GAAGACTTAA AGGAGTTGTC 
     5821 ACCCACTGAA GGAAGGAGTC TCCAAGAGCT TTTGGATTAC CCCGGGGAGG ATGTGGAGGA 
     5881 GACTTTCTGC CTCAACTTCA CGATCTGCCG AGAAAGCTAT GGAGTGATTG AACAGAAGAA 
     5941 GCTGATACCT GGGGGAGATA ATGTAACTGT GTGCAAGGAT AACAGGCAGG AATTTGTGGA 
     6001 TGCTTATGTG AATTATGTCT TCCAAATCTC AGTTCATGAA TGGTACACAG CCTTCTCTAG 
     6061 TGGCTTCCTA AAGGTGTGTG GTGGCAAAGT ACTTGAGCTC TTCCAGCCTT CAGAACTGAG 
     6121 GGCTATGATG GTGGGGAACA GCAACTACAA CTGGGAAGAA CTGCAAGAGA CTGCCATCTA 
     6181 CAAGGGAGAT TACTCGGCCA CACATCCCAC TGTAAAACTA TTTTGGGAAA CATTTCATGA 
     6241 GTTTCCATTG GAAAAGAAGA AGAAGTTTCT CTTGTTCCTG ACAGGCAGCG ATCGGATTCC 
     6301 CATCTACGGC ATGGCCAGTC TGCAGATTGT CATCCAGTCC ACAGCCAGCG GGGAGGAGTA 
     6361 CTTGCCGGTG GCCCACACTT GCTACAACCT TCTTGACCTC CCCAAGTACA GCAGCAAAGA 
     6421 GATTCTGAGT GCCCGGCTGA CCCAGGCCCT TGACAACTAT GAAGGGTTTA GTTTGGCCAC 
     6481 GCGTACGCGG CCGCTCGAGC AGAAACTCAT CTCAGAAGAG GATCTGGCAG CAAATGATAT 
     6541 CCTGGATTAC AAGGATGACG ACGATAAGGT TTAAACGGCC GGCCGCGGTC TGTACAAGTA 
     6601 GGATTCGTCG AGGGACCTAA TAACTTCGTA TAGCATACAT TATACGAAGT TATACATGTT 
     6661 TAAGGGTTCC GGTTCCACTA GGTACAATTC GATATCAAGC TTATCGATAA TCAACCTCTG 
     6721 GATTACAAAA TTTGTGAAAG ATTGACTGGT ATTCTTAACT ATGTTGCTCC TTTTACGCTA 
     6781 TGTGGATACG CTGCTTTAAT GCCTTTGTAT CATGCTATTG CTTCCCGTAT GGCTTTCATT 
     6841 TTCTCCTCCT TGTATAAATC CTGGTTGCTG TCTCTTTATG AGGAGTTGTG GCCCGTTGTC 
     6901 AGGCAACGTG GCGTGGTGTG CACTGTGTTT GCTGACGCAA CCCCCACTGG TTGGGGCATT 
     6961 GCCACCACCT GTCAGCTCCT TTCCGGGACT TTCGCTTTCC CCCTCCCTAT TGCCACGGCG 
     7021 GAACTCATCG CCGCCTGCCT TGCCCGCTGC TGGACAGGGG CTCGGCTGTT GGGCACTGAC 
     7081 AATTCCGTGG TGTTGTCGGG GAAATCATCG TCCTTTCCTT GGCTGCTCGC CTGTGTTGCC 
     7141 ACCTGGATTC TGCGCGGGAC GTCCTTCTGC TACGTCCCTT CGGCCCTCAA TCCAGCGGAC 
     7201 CTTCCTTCCC GCGGCCTGCT GCCGGCTCTG CGGCCTCTTC CGCGTCTTCG CCTTCGCCCT 
     7261 CAGACGAGTC GGATCTCCCT TTGGGCCGCC TCCCCGCATC GATACCGTCG ACCTCGATCG 
     7321 AGACCTAGAA AAACATGGAG CAATCACAAG TAGCAATACA GCAGCTACCA ATGCTGATTG 
     7381 TGCCTGGCTA GAAGCACAAG AGGAGGAGGA GGTGGGTTTT CCAGTCACAC CTCAGGTACC 
     7441 TTTAAGACCA ATGACTTACA AGGCAGCTGT AGATCTTAGC CACTTTTTAA AAGAAAAGGG 
     7501 GGGACTGGAA GGGCTAATTC ACTCCCAACG AAGACAAGAT ATCCTTGATC TGTGGATCTA 
     7561 CCACACACAA GGCTACTTCC CTGATTGGCA GAACTACACA CCAGGGCCAG GGATCAGATA 
     7621 TCCACTGACC TTTGGATGGT GCTACAAGCT AGTACCAGTT GAGCAAGAGA AGGTAGAAGA 
     7681 AGCCAATGAA GGAGAGAACA CCCGCTTGTT ACACCCTGTG AGCCTGCATG GGATGGATGA 
     7741 CCCGGAGAGA GAAGTATTAG AGTGGAGGTT TGACAGCCGC CTAGCATTTC ATCACATGGC 
     7801 CCGAGAGCTG CATCCGGACT GTACTGGGTC TCTCTGGTTA GACCAGATCT GAGCCTGGGA 
     7861 GCTCTCTGGC TAACTAGGGA ACCCACTGCT TAAGCCTCAA TAAAGCTTGC CTTGAGTGCT 
     7921 TCAAGTAGTG TGTGCCCGTC TGTTGTGTGA CTCTGGTAAC TAGAGATCCC TCAGACCCTT 
     7981 TTAGTCAGTG TGGAAAATCT CTAGCAGCAT GTGAGCAAAA GGCCAGCAAA AGGCCAGGAA 
     8041 CCGTAAAAAG GCCGCGTTGC TGGCGTTTTT CCATAGGCTC CGCCCCCCTG ACGAGCATCA 
     8101 CAAAAATCGA CGCTCAAGTC AGAGGTGGCG AAACCCGACA GGACTATAAA GATACCAGGC 
     8161 GTTTCCCCCT GGAAGCTCCC TCGTGCGCTC TCCTGTTCCG ACCCTGCCGC TTACCGGATA 
     8221 CCTGTCCGCC TTTCTCCCTT CGGGAAGCGT GGCGCTTTCT CATAGCTCAC GCTGTAGGTA 
     8281 TCTCAGTTCG GTGTAGGTCG TTCGCTCCAA GCTGGGCTGT GTGCACGAAC CCCCCGTTCA 
     8341 GCCCGACCGC TGCGCCTTAT CCGGTAACTA TCGTCTTGAG TCCAACCCGG TAAGACACGA 
     8401 CTTATCGCCA CTGGCAGCAG CCACTGGTAA CAGGATTAGC AGAGCGAGGT ATGTAGGCGG 
     8461 TGCTACAGAG TTCTTGAAGT GGTGGCCTAA CTACGGCTAC ACTAGAAGAA CAGTATTTGG 
     8521 TATCTGCGCT CTGCTGAAGC CAGTTACCTT CGGAAAAAGA GTTGGTAGCT CTTGATCCGG 
     8581 CAAACAAACC ACCGCTGGTA GCGGTGGTTT TTTTGTTTGC AAGCAGCAGA TTACGCGCAG 
     8641 AAAAAAAGGA TCTCAAGAAG ATCCTTTGAT CTTTTCTACG GGGTCTGACG CTCAGTGGAA 
     8701 CGAAAACTCA CGTTAAGGGA TTTTGGTCAT GATTACGCCC CGCCCTGCCA CTCATCGCAG 
     8761 TACTGTTGTA ATTCATTAAG CATTCTGCCG ACATGGAAGC CATCACAAAC GGCATGATGA 
     8821 ACCTGAATCG CCAGCGGCAT CAGCACCTTG TCGCCTTGCG TATAATATTT GCCCATGGTG 
     8881 AAAACGGGGG CGAAGAAGTT GTCCATATTG GCCACGTTTA AATCAAAACT GGTGAAACTC 
     8941 ACCCAGGGAT TGGCTGAGAC GAAAAACATA TTCTCAATAA ACCCTTTAGG GAAATAGGCC 
     9001 AGGTTTTCAC CGTAACACGC CACATCTTGC GAATATATGT GTAGAAACTG CCGGAAATCG 
     9061 TCGTGGTATT CACTCCAGAG CGATGAAAAC GTTTCAGTTT GCTCATGGAA AACGGTGTAA 
     9121 CAAGGGTGAA CACTATCCCA TATCACCAGC TCACCGTCTT TCATTGCCAT ACGGAACTCC 
     9181 GGATGAGCAT TCATCAGGCG GGCAAGAATG TGAATAAAGG CCGGATAAAA CTTGTGCTTA 
     9241 TTTTTCTTTA CGGTCTTTAA AAAGGCCGTA ATATCCAGCT GAACGGTCTG GTTATAGGTA 
     9301 CATTGAGCAA CTGACTGAAA TGCCTCAAAA TGTTCTTTAC GATGCCATTG GGATATATCA 
     9361 ACGGTGGTAT ATCCAGTGAT TTTTTTCTCC ATACTCTTCC TTTTTCAATA TTATTGAAGC 
     9421 ATTTATCAGG GTTATTGTCT CATGAGCGGA TACATATTTG AATGTATTTA GAAAAATAAA 
     9481 CAAATAGGGG TCCCGCGCAC ATTTCCCCGA AAAGTGCCAC CTGAC
//