LOCUS       RC214022                8331 bp    DNA     circular  SYN 
DEFINITION  SLC12A6 (Myc-DDK-tagged)-Human solute carrier family 12 (potassium/chloride transporters), member 6 (SLC12A6), transcript variant 1
ACCESSION   NM_133647
VERSION     NM_133647.1
SOURCE      synthetic construct 
FEATURES    Location/Qualifiers 
     misc_feature    201..924 
                     /label="CMV promoter"  
     misc_feature    839..856 
                     /label="Restriction Enzyme"  
     misc_feature    953..971 
                     /label="T7 promoter"  
     misc_feature    4488..4579 
                     /label="Myc-DDK"  
     misc_feature    4609..5194 
                     /label="PolyA signal"  
     misc_feature    4666..4685 
                     /label="Restriction Enzyme"  
     misc_feature    5470..6142 
                     /label="ColE1"  
     misc_feature    6572..7366 
                     /label="Kan/Neo"  
     misc_feature    7401..7703 
                     /label="SV40 ori"  
     misc_feature    7789..8266 
                     /label="f1 ori"  
     misc_feature    1020..1028 
                     /label="Restriction Enzyme"  
     misc_feature    4470..4476 
                     /label="Restriction Enzyme"  
     misc_feature    1020..4470 
                     /label="SLC12A6"  
ORIGIN                      
        1 AACAAAATAT TAACGCTTAC AATTTCCATT CGCCATTCAG GCTGCGCAAC TGTTGGGAAG 
       61 GGCGATCGGT GCGGGCCTCT TCGCTATTAC GCCAGCTGGC GAAAGGGGGA TGTGCTGCAA 
      121 GGCGATTAAG TTGGGTAACG CCAGGGTTTT CCCAGTCACG ACGTTGTAAA ACGACGGCCA 
      181 GTGCCAAGCT GATCTATACA TTGAATCAAT ATTGGCAATT AGCCATATTA GTCATTGGTT 
      241 ATATAGCATA AATCAATATT GGCTATTGGC CATTGCATAC GTTGTATCTA TATCATAATA 
      301 TGTACATTTA TATTGGCTCA TGTCCAATAT GACCGCCATG TTGACATTGA TTATTGACTA 
      361 GTTATTAATA GTAATCAATT ACGGGGTCAT TAGTTCATAG CCCATATATG GAGTTCCGCG 
      421 TTACATAACT TACGGTAAAT GGCCCGCCTG GCTGACCGCC CAACGACCCC CGCCCATTGA 
      481 CGTCAATAAT GACGTATGTT CCCATAGTAA CGCCAATAGG GACTTTCCAT TGACGTCAAT 
      541 GGGTGGAGTA TTTACGGTAA ACTGCCCACT TGGCAGTACA TCAAGTGTAT CATATGCCAA 
      601 GTCCGCCCCC TATTGACGTC AATGACGGTA AATGGCCCGC CTGGCATTAT GCCCAGTACA 
      661 TGACCTTACG GGACTTTCCT ACTTGGCAGT ACATCTACGT ATTAGTCATC GCTATTACCA 
      721 TGGTGATGCG GTTTTGGCAG TACACCAATG GGCGTGGATA GCGGTTTGAC TCACGGGGAT 
      781 TTCCAAGTCT CCACCCCATT GACGTCAATG GGAGTTTGTT TTGGCACCAA AATCAACGGG 
      841 ACTTTCCAAA ATGTCGTAAT AACCCCGCCC CGTTGACGCA AATGGGCGGT AGGCGTGTAC 
      901 GGTGGGAGGT CTATATAAGC AGAGCTCGTT TAGTGAACCG TCAGAATTTT GTAATACGAC 
      961 TCACTATAGG GCGGCCGGGA ATTCGTCGAC TGGATCCGGT ACCGAGGAGA TCTGCCGCCG 
     1021 CGATCGCCAT GCATCCTCCA GAAACCACCA CCAAGATGGC TTCAGTTCGG TTCATGGTGA 
     1081 CACCGACAAA GATCGATGAC ATTCCAGGTT TGTCAGACAC CAGTCCGGAC CTCAGCTCTC 
     1141 GATCTAGTTC CCGAGTAAGA TTTAGCTCCC GGGAAAGCGT GCCTGAAACA AGCCGGAGTG 
     1201 AGCCTATGAG TGAGATGTCT GGGGCCACCA CTTCGCTGGC AACTGTTGCA CTGGATCCAC 
     1261 CCAGTGACCG GACTTCTCAC CCCCAGGATG TCATCGAGGA CCTGAGTCAG AACTCCATCA 
     1321 CAGGGGAACA CAGCCAACTG TTAGACGACG GACATAAGAA AGCTCGAAAT GCTTATCTCA 
     1381 ATAATTCCAA TTATGAAGAA GGAGATGAAT ATTTTGATAA AAATTTGGCA CTCTTTGAGG 
     1441 AAGAAATGGA CACCAGACCG AAGGTGTCTT CCCTCCTCAA CCGCATGGCC AATTACACTA 
     1501 ATCTGACTCA AGGAGCAAAG GAACATGAAG AGGCAGAAAA CATCACTGAA GGGAAAAAGA 
     1561 AGCCCACCAA GACCCCCCAA ATGGGTACCT TCATGGGTGT CTACCTCCCA TGTCTACAAA 
     1621 ATATTTTTGG AGTGATCCTT TTTTTACGCC TTACATGGGT GGTGGGCACA GCTGGAGTTC 
     1681 TTCAGGCTTT TGCAATTGTC CTTATCTGCT GCTGCTGTAC AATGTTGACT GCTATCTCCA 
     1741 TGAGTGCCAT TGCCACTAAT GGAGTGGTGC CAGCTGGGGG CTCATACTTT ATGATTTCCC 
     1801 GGGCACTGGG CCCAGAGTTT GGTGGGGCTG TTGGCCTCTG CTTTTATCTT GGTACCACAT 
     1861 TTGCAGCAGC CATGTACATC CTTGGTGCCA TTGAAATCTT TCTGGTCTAT ATCGTCCCCC 
     1921 GAGCTGCCAT CTTTCACAGT GATGACGCAC TCAAGGAATC AGCAGCCATG CTAAATAACA 
     1981 TGCGTGTCTA CGGCACAGCT TTCTTGGTCC TTATGGTATT AGTGGTATTT ATCGGCGTAC 
     2041 GCTATGTGAA CAAGTTTGCC TCACTTTTCC TGGCCTGTGT CATTGTGTCC ATCTTGGCCA 
     2101 TCTATGCTGG AGCCATCAAG TCTTCTTTTG CTCCTCCACA CTTCCCGGTC TGCATGCTGG 
     2161 GTAACCGCAC CCTTTCATCA AGACACATTG ACGTTTGCTC TAAGACCAAG GAAATTAACA 
     2221 ACATGACAGT CCCATCAAAG TTATGGGGAT TCTTCTGTAA CTCGAGTCAA TTTTTCAATG 
     2281 CCACCTGTGA TGAATACTTT GTTCACAATA ACGTCACTTC AATCCAGGGC ATTCCTGGAT 
     2341 TGGCTAGTGG TATAATTACA GAGAATCTTT GGAGTAATTA CCTACCCAAG GGAGAGATCA 
     2401 TCGAAAAGCC TTCAGCCAAA TCTTCTGATG TCTTAGGCAG CTTAAACCAT GAATATGTTC 
     2461 TTGTTGACAT CACCACCTCC TTCACGCTTC TGGTGGGAAT CTTCTTTCCC TCTGTTACAG 
     2521 GTATCATGGC TGGATCAAAC AGATCTGGAG ATCTGAAAGA TGCTCAGAAG TCTATTCCGA 
     2581 TTGGTACTAT CCTTGCCATC CTGACCACCT CCTTTGTTTA TTTAAGCAAT GTTGTCCTTT 
     2641 TTGGTGCATG TATTGAAGGG GTTGTTCTCA GAGACAAGTT CGGTGATGCT GTGAAAGGTA 
     2701 ATTTGGTGGT AGGCACCTTA TCTTGGCCAT CCCCATGGGT GATTGTTATT GGCTCCTTCT 
     2761 TTTCAACATG TGGGGCTGGA CTTCAGAGCC TCACAGGTGC ACCGAGGCTG CTACAAGCTA 
     2821 TTGCCAAGGA TAACATCATA CCGTTTCTGA GGGTTTTTGG CCACAGCAAA GCCAATGGGG 
     2881 AACCTACCTG GGCTTTACTT CTAACTGCTG CCATTGCAGA GCTTGGAATA CTCATTGCCT 
     2941 CCCTGGATCT TGTGGCCCCA ATTCTTTCCA TGTTTTTTCT CATGTGTTAC CTCTTTGTAA 
     3001 ACTTGGCATG TGCCTTGCAA ACATTACTTC GAACACCCAA CTGGAGACCC CGATTCCGCT 
     3061 ACTACCATTG GGCCCTTTCT TTCATGGGAA TGAGTATCTG TCTGGCTCTG ATGTTCATTT 
     3121 CTTCCTGGTA TTATGCCATT GTAGCCATGG TAATAGCTGG TATGATCTAC AAGTACATTG 
     3181 AATACCAAGG AGCTGAGAAA GAATGGGGTG ATGGTATCCG TGGGCTGTCC CTCAGTGCAG 
     3241 CCCGGTTTGC TTTGCTTCGA TTGGAGGAAG GACCTCCACA CACTAAAAAC TGGAGGCCTC 
     3301 AGTTGCTTGT ATTACTGAAA CTAGATGAAG ACTTACATGT CAAGCATCCT CGCCTCCTCA 
     3361 CCTTTGCCTC ACAGCTCAAA GCAGGAAAAG GTCTCACTAT TGTGGGCTCT GTCATCGTGG 
     3421 GGAACTTCCT AGAGAACTAC GGTGAAGCTT TAGCTGCTGA GCAGACCATA AAGCACCTAA 
     3481 TGGAGGCAGA GAAGGTAAAA GGATTCTGCC AGCTGGTGGT GGCCGCCAAG CTGAGAGAGG 
     3541 GCATTTCCCA CCTCATCCAG TCATGTGGCC TTGGGGGCAT GAAGCACAAC ACGGTGGTGA 
     3601 TGGGCTGGCC TAATGGCTGG CGTCAAAGCG AAGATGCCCG CGCTTGGAAG ACTTTTATTG 
     3661 GCACAGTTCG AGTGACAACT GCTGCCCATC TTGCACTGCT GGTGGCTAAA AACATCTCCT 
     3721 TCTTTCCCAG CAATGTGGAG CAATTTTCTG AGGGCAACAT TGATGTGTGG TGGATTGTGC 
     3781 ATGATGGGGG GATGCTTATG CTACTACCAT TCCTACTGAA ACAGCACAAG GTGTGGCGAA 
     3841 AGTGCAGCAT ACGGATCTTC ACAGTAGCCC AATTAGAAGA CAACAGTATC CAAATGAAGA 
     3901 AGGACCTAGC CACCTTCCTA TATCACTTAC GCATTGAGGC GGAGGTAGAA GTGGTGGAGA 
     3961 TGCATGACAG TGATATATCA GCATATACTT ACGAGCGCAC TTTGATGATG GAACAAAGGT 
     4021 CCCAGATGCT CCGGCACATG CGGCTATCCA AAACAGAGCG AGACAGAGAG GCACAATTGG 
     4081 TGAAAGACCG AAACTCAATG CTACGATTGA CCAGCATTGG CTCTGATGAG GACGAAGAGA 
     4141 CAGAAACCTA TCAGGAGAAG GTGCACATGA CTTGGACAAA AGACAAGTAC ATGGCATCCC 
     4201 GGGGACAAAA AGCGAAGTCA ATGGAAGGAT TCCAGGACCT GCTTAACATG CGTCCGGACC 
     4261 AGTCCAATGT GAGGCGGATG CATACAGCAG TGAAACTCAA CGAGGTTATA GTTAACAAGT 
     4321 CCCATGAAGC AAAGCTGGTT TTATTGAATA TGCCAGGGCC ACCCCGAAAC CCTGAGGGTG 
     4381 ATGAAAACTA CATGGAGTTC CTAGAGGTGC TTACCGAGGG ACTAGAGCGA GTCCTACTTG 
     4441 TCCGGGGTGG TGGCAGTGAA GTGATCACCA TTTATTCAAC GCGTACGCGG CCGCTCGAGC 
     4501 AGAAACTCAT CTCAGAAGAG GATCTGGCAG CAAATGATAT CCTGGATTAC AAGGATGACG 
     4561 ACGATAAGGT TTAAACGGCC GGCCGCGGTC ATAGCTGTTT CCTGAACAGA TCCCGGGTGG 
     4621 CATCCCTGTG ACCCCTCCCC AGTGCCTCTC CTGGCCCTGG AAGTTGCCAC TCCAGTGCCC 
     4681 ACCAGCCTTG TCCTAATAAA ATTAAGTTGC ATCATTTTGT CTGACTAGGT GTCCTTCTAT 
     4741 AATATTATGG GGTGGAGGGG GGTGGTATGG AGCAAGGGGC AAGTTGGGAA GACAACCTGT 
     4801 AGGGCCTGCG GGGTCTATTG GGAACCAAGC TGGAGTGCAG TGGCACAATC TTGGCTCACT 
     4861 GCAATCTCCG CCTCCTGGGT TCAAGCGATT CTCCTGCCTC AGCCTCCCGA GTTGTTGGGA 
     4921 TTCCAGGCAT GCATGACCAG GCTCAGCTAA TTTTTGTTTT TTTGGTAGAG ACGGGGTTTC 
     4981 ACCATATTGG CCAGGCTGGT CTCCAACTCC TAATCTCAGG TGATCTACCC ACCTTGGCCT 
     5041 CCCAAATTGC TGGGATTACA GGCGTGAACC ACTGCTCCCT TCCCTGTCCT TCTGATTTTA 
     5101 AAATAACTAT ACCAGCAGGA GGACGTCCAG ACACAGCATA GGCTACCTGG CCATGCCCAA 
     5161 CCGGTGGGAC ATTTGAGTTG CTTGCTTGGC ACTGTCCTCT CATGCGTTGG GTCCACTCAG 
     5221 TAGATGCCTG TTGAATTGGG TACGCGGCCA GCGGCGAGCG GTATCAGCTC ACTCAAAGGC 
     5281 GGTAATACGG TTATCCACAG AATCAGGGGA TAACGCAGGA AAGAACATGT GAGCAAAAGG 
     5341 CCAGCAAAAG GCCAGGAACC GTAAAAAGGC CGCGTTGCTG GCGTTTTTCC ATAGGCTCCG 
     5401 CCCCCCTGAC GAGCATCACA AAAATCGACG CTCAAGTCAG AGGTGGCGAA ACCCGACAGG 
     5461 ACTATAAAGA TACCAGGCGT TTCCCCCTGG AAGCTCCCTC GTGCGCTCTC CTGTTCCGAC 
     5521 CCTGCCGCTT ACCGGATACC TGTCCGCCTT TCTCCCTTCG GGAAGCGTGG CGCTTTCTCA 
     5581 TAGCTCACGC TGTAGGTATC TCAGTTCGGT GTAGGTCGTT CGCTCCAAGC TGGGCTGTGT 
     5641 GCACGAACCC CCCGTTCAGC CCGACCGCTG CGCCTTATCC GGTAACTATC GTCTTGAGTC 
     5701 CAACCCGGTA AGACACGACT TATCGCCACT GGCAGCAGCC ACTGGTAACA GGATTAGCAG 
     5761 AGCGAGGTAT GTAGGCGGTG CTACAGAGTT CTTGAAGTGG TGGCCTAACT ACGGCTACAC 
     5821 TAGAAGAACA GTATTTGGTA TCTGCGCTCT GCTGAAGCCA GTTACCTTCG GAAAAAGAGT 
     5881 TGGTAGCTCT TGATCCGGCA AACAAACCAC CGCTGGTAGC GGTGGTTTTT TTGTTTGCAA 
     5941 GCAGCAGATT ACGCGCAGAA AAAAAGGATC TCAAGAAGAT CCTTTGATCT TTTCTACGGG 
     6001 GTCTGACGCT CAGTGGAACG AAAACTCACG TTAAGGGATT TTGGTCATGA GATTATCAAA 
     6061 AAGGATCTTC ACCTAGATCC TTTTAAATTA AAAATGAAGT TTTAAATCAA TCTAAAGTAT 
     6121 ATATGAGTAA CCTGAGGCTA TGGCAGGGCC TGCCGCCCCG ACGTTGGCTG CGAGCCCTGG 
     6181 GCCTTCACCC GAACTTGGGG GGTGGGGTGG GGAAAAGGAA GAAACGCGGG CGTATTGGCC 
     6241 CCAATGGGGT CTCGGTGGGG TATCGACAGA GTGCCAGCCC TGGGACCGAA CCCCGCGTTT 
     6301 ATGAACAAAC GACCCAACAC CGTGCGTTTT ATTCTGTCTT TTTATTGCCG TCATAGCGCG 
     6361 GGTTCCTTCC GGTATTGTCT CCTTCCGTGT TTCAGTTAGC CTCCCCCTAG GGTGGGCGAA 
     6421 GAACTCCAGC ATGAGATCCC CGCGCTGGAG GATCATCCAG CCGGCGTCCC GGAAAACGAT 
     6481 TCCGAAGCCC AACCTTTCAT AGAAGGCGGC GGTGGAATCG AAATCTCGTG ATGGCAGGTT 
     6541 GGGCGTCGCT TGGTCGGTCA TTTCGAACCC CAGAGTCCCG CTCAGAAGAA CTCGTCAAGA 
     6601 AGGCGATAGA AGGCGATGCG CTGCGAATCG GGAGCGGCGA TACCGTAAAG CACGAGGAAG 
     6661 CGGTCAGCCC ATTCGCCGCC AAGCTCTTCA GCAATATCAC GGGTAGCCAA CGCTATGTCC 
     6721 TGATAGCGAT CCGCCACACC CAGCCGGCCA CAGTCGATGA ATCCAGAAAA GCGGCCATTT 
     6781 TCCACCATGA TATTCGGCAA GCAGGCATCG CCATGGGTCA CGACGAGATC CTCGCCGTCG 
     6841 GGCATGCTCG CCTTGAGCCT GGCGAACAGT TCGGCTGGCG CGAGCCCCTG ATGCTCTTCG 
     6901 TCCAGATCAT CCTGATCGAC AAGACCGGCT TCCATCCGAG TACGTGCTCG CTCGATGCGA 
     6961 TGTTTCGCTT GGTGGTCGAA TGGGCAGGTA GCCGGATCAA GCGTATGCAG CCGCCGCATT 
     7021 GCATCAGCCA TGATGGATAC TTTCTCGGCA GGAGCAAGGT GAGATGACAG GAGATCCTGC 
     7081 CCCGGCACTT CGCCCAATAG CAGCCAGTCC CTTCCCGCTT CAGTGACAAC GTCGAGCACA 
     7141 GCTGCGCAAG GAACGCCCGT CGTGGCCAGC CACGATAGCC GCGCTGCCTC GTCTTGCAGT 
     7201 TCATTCAGGG CACCGGACAG GTCGGTCTTG ACAAAAAGAA CCGGGCGCCC CTGCGCTGAC 
     7261 AGCCGGAACA CGGCGGCATC AGAGCAGCCG ATTGTCTGTT GTGCCCAGTC ATAGCCGAAT 
     7321 AGCCTCTCCA CCCAAGCGGC CGGAGAACCT GCGTGCAATC CATCTTGTTC AATCATGCGA 
     7381 AACGATCCTC ATCCTGTCTC TTGATCGATC TTTGCAAAAG CCTAGGCCTC CAAAAAAGCC 
     7441 TCCTCACTAC TTCTGGAATA GCTCAGAGGC CGAGGCGGCC TCGGCCTCTG CATAAATAAA 
     7501 AAAAATTAGT CAGCCATGGG GCGGAGAATG GGCGGAACTG GGCGGAGTTA GGGGCGGGAT 
     7561 GGGCGGAGTT AGGGGCGGGA CTATGGTTGC TGACTAATTG AGATGCATGC TTTGCATACT 
     7621 TCTGCCTGCT GGGGAGCCTG GGGACTTTCC ACACCTGGTT GCTGACTAAT TGAGATGCAT 
     7681 GCTTTGCATA CTTCTGCCTG CTGGGGAGCC TGGGGACTTT CCACACCCTA ACTGACACAC 
     7741 ATTCCACAGC TGGTTCTTTC CGCCTCAGGA CTCTTCCTTT TTCAATATTA TTGAAGCATT 
     7801 TATCAGGGTT ATTGTCTCAT GAGCGGATAC ATATTTGAAT GTATTTAGAA AAATAAACAA 
     7861 ATAGGGGTTC CGCGCACATT TCCCCGAAAA GTGCCACCTG ACGCGCCCTG TAGCGGCGCA 
     7921 TTAAGCGCGG CGGGTGTGGT GGTTACGCGC AGCGTGACCG CTACACTTGC CAGCGCCCTA 
     7981 GCGCCCGCTC CTTTCGCTTT CTTCCCTTCC TTTCTCGCCA CGTTCGCCGG CTTTCCCCGT 
     8041 CAAGCTCTAA ATCGGGGGCT CCCTTTAGGG TTCCGATTTA GTGCTTTACG GCACCTCGAC 
     8101 CCCAAAAAAC TTGATTAGGG TGATGGTTCA CGTAGTGGGC CATCGCCCTG ATAGACGGTT 
     8161 TTTCGCCCTT TGACGTTGGA GTCCACGTTC TTTAATAGTG GACTCTTGTT CCAAACTGGA 
     8221 ACAACACTCA ACCCTATCTC GGTCTATTCT TTTGATTTAT AAGGGATTTT GCCGATTTCG 
     8281 GCCTATTGGT TAAAAAATGA GCTGATTTAA CAAAAATTTA ACGCGAATTT T
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