LOCUS       RC213999                8889 bp    DNA     circular  SYN 
DEFINITION  KDM2B (Myc-DDK-tagged)-Human lysine (K)-specific demethylase 2B (KDM2B), transcript variant 1
ACCESSION   NM_032590
VERSION     NM_032590.3
SOURCE      synthetic construct 
FEATURES    Location/Qualifiers 
     misc_feature    201..924 
                     /label="CMV promoter"  
     misc_feature    839..856 
                     /label="Restriction Enzyme"  
     misc_feature    953..971 
                     /label="T7 promoter"  
     misc_feature    5046..5137 
                     /label="Myc-DDK"  
     misc_feature    5167..5752 
                     /label="PolyA signal"  
     misc_feature    5224..5243 
                     /label="Restriction Enzyme"  
     misc_feature    6028..6700 
                     /label="ColE1"  
     misc_feature    7130..7924 
                     /label="Kan/Neo"  
     misc_feature    7959..8261 
                     /label="SV40 ori"  
     misc_feature    8347..8824 
                     /label="f1 ori"  
     misc_feature    1020..1028 
                     /label="Restriction Enzyme"  
     misc_feature    5028..5034 
                     /label="Restriction Enzyme"  
     misc_feature    1020..5028 
                     /label="KDM2B"  
ORIGIN                      
        1 AACAAAATAT TAACGCTTAC AATTTCCATT CGCCATTCAG GCTGCGCAAC TGTTGGGAAG 
       61 GGCGATCGGT GCGGGCCTCT TCGCTATTAC GCCAGCTGGC GAAAGGGGGA TGTGCTGCAA 
      121 GGCGATTAAG TTGGGTAACG CCAGGGTTTT CCCAGTCACG ACGTTGTAAA ACGACGGCCA 
      181 GTGCCAAGCT GATCTATACA TTGAATCAAT ATTGGCAATT AGCCATATTA GTCATTGGTT 
      241 ATATAGCATA AATCAATATT GGCTATTGGC CATTGCATAC GTTGTATCTA TATCATAATA 
      301 TGTACATTTA TATTGGCTCA TGTCCAATAT GACCGCCATG TTGACATTGA TTATTGACTA 
      361 GTTATTAATA GTAATCAATT ACGGGGTCAT TAGTTCATAG CCCATATATG GAGTTCCGCG 
      421 TTACATAACT TACGGTAAAT GGCCCGCCTG GCTGACCGCC CAACGACCCC CGCCCATTGA 
      481 CGTCAATAAT GACGTATGTT CCCATAGTAA CGCCAATAGG GACTTTCCAT TGACGTCAAT 
      541 GGGTGGAGTA TTTACGGTAA ACTGCCCACT TGGCAGTACA TCAAGTGTAT CATATGCCAA 
      601 GTCCGCCCCC TATTGACGTC AATGACGGTA AATGGCCCGC CTGGCATTAT GCCCAGTACA 
      661 TGACCTTACG GGACTTTCCT ACTTGGCAGT ACATCTACGT ATTAGTCATC GCTATTACCA 
      721 TGGTGATGCG GTTTTGGCAG TACACCAATG GGCGTGGATA GCGGTTTGAC TCACGGGGAT 
      781 TTCCAAGTCT CCACCCCATT GACGTCAATG GGAGTTTGTT TTGGCACCAA AATCAACGGG 
      841 ACTTTCCAAA ATGTCGTAAT AACCCCGCCC CGTTGACGCA AATGGGCGGT AGGCGTGTAC 
      901 GGTGGGAGGT CTATATAAGC AGAGCTCGTT TAGTGAACCG TCAGAATTTT GTAATACGAC 
      961 TCACTATAGG GCGGCCGGGA ATTCGTCGAC TGGATCCGGT ACCGAGGAGA TCTGCCGCCG 
     1021 CGATCGCCAT GGCGGGTCCG CAAATGGGGG GATCTGCAGA GGATCACCCC CCACGAAAAA 
     1081 GACATGCAGC AGAAAAGCAA AAAAAGAAAA CAGTTATATA TACAAAATGC TTTGAATTTG 
     1141 AGTCGGCCAC ACAGCGCCCG ATTGACCGCC AGCGATACGA CGAGAACGAG GACTTGTCGG 
     1201 ACGTGGAGGA GATCGTCAGC GTCCGCGGCT TCAGCCTGGA GGAGAAGCTT CGCAGCCAGC 
     1261 TGTACCAGGG GGACTTCGTG CACGCCATGG AGGGCAAAGA TTTCAACTAT GAGTACGTAC 
     1321 AGAGAGAAGC TCTCAGGGTT CCCCTGATAT TTCGAGAAAA GGATGGACTG GGAATTAAGA 
     1381 TGCCTGACCC TGATTTCACA GTCCGAGACG TCAAACTCCT AGTGGGGAGC CGGCGGCTTG 
     1441 TGGACGTGAT GGATGTGAAC ACCCAGAAGG GCACGGAGAT GAGCATGTCC CAGTTTGTGC 
     1501 GTTACTACGA GACGCCCGAG GCCCAGCGGG ACAAGCTGTA CAACGTCATC AGCCTAGAGT 
     1561 TCAGCCACAC CAAGCTGGAG CACTTGGTCA AGCGTCCGAC TGTGGTAGAC CTGGTGGACT 
     1621 GGGTGGACAA CATGTGGCCC CAGCATCTGA AGGAGAAGCA GACAGAAGCC ACGAACGCCA 
     1681 TTGCAGAGAT GAAGTACCCG AAAGTGAAAA AGTACTGTCT GATGAGCGTG AAAGGTTGTT 
     1741 TCACCGACTT CCACATCGAC TTTGGAGGCA CTTCCGTTTG GTACCATGTT TTCCGGGGTG 
     1801 GGAAGATTTT TTGGCTGATT CCTCCAACGC TGCACAATTT GGCGCTGTAC GAGGAGTGGG 
     1861 TGCTGTCAGG CAAACAGAGT GACATCTTTC TGGGAGACCG TGTGGAACGA TGCCAAAGAA 
     1921 TTGAGCTGAA GCAGGGCTAC ACATTTTTCA TCCCTTCCGG TTGGATCCAT GCCGTCTACA 
     1981 CCCCTGTAGA CTCTTTGGTG TTCGGCGGAA ACATCCTGCA CAGCTTTAAC GTGCCCATGC 
     2041 AGCTGCGGAT CTACGAGATC GAGGACAGGA CGCGGGTGCA GCCCAAATTC CGTTACCCCT 
     2101 TCTACTATGA GATGTGCTGG TATGTCCTGG AGAGATACGT GTACTGTGTG ACCCAGCGCT 
     2161 CCCACCTCAC TCAGGAATAC CAGAGGGAGT CGATGCTTAT TGATGCCCCG AGGAAGCCCA 
     2221 GCATAGACGG CTTCTCTTCG GATTCCTGGC TGGAGATGGA GGAGGAGGCC TGTGATCAGC 
     2281 AGCCTCAGGA GGAGGAGGAG AAGGACGAGG AGGGCGAGGG CAGGGACAGG GCACCCAAAC 
     2341 CGCCCACCGA TGGCTCCACT TCACCCACCA GCACGCCCTC TGAGGACCAG GAGGCCCTCG 
     2401 GGAAGAAGCC CAAAGCACCT GCCCTGCGAT TCCTCAAAAG GACTTTGTCT AATGAGTCGG 
     2461 AGGAAAGTGT GAAGTCCACC ACATTGGCCG TAGACTACCC CAAGACCCCC ACCGGCTCTC 
     2521 CCGCCACGGA GGTCTCTGCC AAATGGACCC ATCTCACTGA GTTTGAACTG AAGGGCCTGA 
     2581 AAGCTCTGGT GGAGAAACTG GAATCCCTCC CGGAGAACAA GAAGTGTGTC CCCGAGGGCA 
     2641 TCGAGGACCC CCAGGCACTC CTGGAGGGTG TGAAGAACGT CCTGAAGGAG CACGCAGATG 
     2701 ATGACCCTAG TCTGGCCATC ACTGGGGTCC CTGTGGTGAC TTGGCCAAAG AAGACTCCAA 
     2761 AGAACCGGGC TGTGGGTCGG CCCAAGGGGA AGCTGGGCCC GGCCTCCGCG GTGAAGTTGG 
     2821 CCGCCAACCG GACAACGGCA GGAGCTCGGC GGCGCCGGAC GCGATGCCGC AAGTGCGAGG 
     2881 CCTGCCTGCG GACCGAGTGC GGAGAGTGCC ACTTCTGCAA GGACATGAAG AAGTTCGGGG 
     2941 GCCCCGGGCG CATGAAGCAG AGCTGCATCA TGCGGCAGTG CATCGCGCCA GTGCTGCCCC 
     3001 ACACCGCCGT GTGCCTTGTG TGTGGCGAGG CGGGGAAGGA AGACACGGTG GAAGAGGAGG 
     3061 AAGGCAAGTT TAACCTCATG CTCATGGAGT GCTCCATCTG CAATGAAATC ATCCACCCTG 
     3121 GATGCCTTAA GATTAAGGAG TCAGAGGGTG TGGTCAACGA CGAGCTTCCA AACTGCTGGG 
     3181 AGTGTCCGAA GTGTAACCAC GCCGGCAAGA CCGGGAAACA AAAGCGTGGC CCTGGCTTTA 
     3241 AGTACGCCTC CAACCTGCCC GGCTCCCTGC TCAAGGAGCA GAAGATGAAC CGGGACAACA 
     3301 AGGAAGGGCA GGAACCTGCC AAGCGGAGGA GTGAGTGTGA GGAGGCGCCC CGGCGCAGGT 
     3361 CGGATGAGCA CTCGAAGAAG GTGCCGCCGG ACGGCCTTCT GCGCAGAAAG TCTGACGACG 
     3421 TGCACCTGAG GAAGAAGCGG AAATACGAGA AGCCCCAGGA GCTGAGTGGA CGCAAGCGGG 
     3481 CCTCATCGCT TCAAACGTCC CCCGGTTCCT CCTCTCACCT CTCGCCGAGG CCCCCTCTAG 
     3541 GCAGCAGCCT CAGCCCCTGG TGGAGATCCA GTCTCACTTA CTTCCAGCAG CAGCTCAAAC 
     3601 CTGGCAAAGA AGATAAGCTT TTCAGGAAAA AGCGGCGGTC CTGGAAGAAC GCCGAGGACC 
     3661 GCATGGCGCT GGCCAACAAG CCCCTCCGGC GCTTCAAGCA GGAACCCGAG GACGAACTGC 
     3721 CCGAGGCGCC CCCCAAGACC AGGGAGAGCG ACCACTCCCG CTCCAGCTCC CCCACCGCGG 
     3781 GACCCAGCAC CGAAGGGGCC GAGGGCCCGG AGGAGAAGAA GAAGGTGAAG ATGCGCCGGA 
     3841 AGCGGCGGCT TCCCAACAAG GAGCTGAGCA GGGAGCTGAG CAAGGAGCTC AACCACGAGA 
     3901 TCCAGAGGAC GGAGAACAGC CTGGCCAACG AGAACCAGCA GCCCATCAAG TCGGAGCCTG 
     3961 AGAGCGAGGG CGAGGAGCCC AAGCGGCCCC CGGGCATCTG CGAGCGTCCC CACCGCTTCA 
     4021 GCAAGGGGCT CAACGGCACC CCCCGGGAGC TGCGGCACCA GCTGGGGCCC AGCCTGCGCA 
     4081 GCCCGCCCCG TGTCATCTCC CGGCCCCCAC CCTCCGTGTC CCCGCCCAAG TGTATCCAGA 
     4141 TGGAGCGCCA TGTGATCCGG CCACCCCCCA TCAGCCCCCC GCCTGACTCG CTACCCCTGG 
     4201 ACGATGGGGC AGCCCACGTC ATGCACAGGG AGGTGTGGAT GGCCGTCTTC AGCTACCTCA 
     4261 GCCACCAAGA CCTGTGTGTG TGCATGCGGG TCTGCAGGAC CTGGAACCGC TGGTGCTGCG 
     4321 ATAAGCGGTT GTGGACCCGC ATTGACCTGA ACCACTGCAA GTCTATCACA CCCCTGATGC 
     4381 TGAGTGGCAT CATCCGGCGA CAGCCCGTCT CCCTCGACCT CAGCTGGACC AATATCTCCA 
     4441 AGAAGCAGCT GAGCTGGCTC ATCAACCGGC TGCCTGGGCT CCGGGACTTG GTGCTGTCAG 
     4501 GCTGCTCATG GATCGCGGTC TCGGCCCTTT GCAGCTCCAG TTGTCCGCTG CTCCGGACCC 
     4561 TGGATGTCCA GTGGGTGGAG GGACTAAAGG ATGCCCAGAT GCGGGATCTC CTGTCCCCGC 
     4621 CCACAGACAA CAGGCCAGGT CAGATGGACA ATCGGAGCAA GCTCCGGAAC ATCGTGGAGC 
     4681 TGCGCCTGGC AGGCCTGGAC ATCACAGATG CCTCCCTGCG GCTCATCATC CGCCACATGC 
     4741 CCCTGCTCTC CAAGCTCCAC CTCAGTTACT GTAACCACGT CACCGACCAG TCTATCAACC 
     4801 TGCTCACTGC TGTTGGCACC ACCACCCGAG ACTCCTTAAC CGAGATCAAC CTGTCTGACT 
     4861 GCAATAAGGT CACTGATCAG TGCCTGTCCT TCTTCAAACG CTGTGGAAAC ATCTGTCATA 
     4921 TTGACCTGAG GTACTGCAAG CAAGTCACCA AGGAAGGCTG TGAGCAGTTC ATAGCCGAGA 
     4981 TGTCTGTGAG TGTCCAGTTT GGGCAAGTAG AAGAAAAACT CCTGCAAAAA CTGAGTACGC 
     5041 GTACGCGGCC GCTCGAGCAG AAACTCATCT CAGAAGAGGA TCTGGCAGCA AATGATATCC 
     5101 TGGATTACAA GGATGACGAC GATAAGGTTT AAACGGCCGG CCGCGGTCAT AGCTGTTTCC 
     5161 TGAACAGATC CCGGGTGGCA TCCCTGTGAC CCCTCCCCAG TGCCTCTCCT GGCCCTGGAA 
     5221 GTTGCCACTC CAGTGCCCAC CAGCCTTGTC CTAATAAAAT TAAGTTGCAT CATTTTGTCT 
     5281 GACTAGGTGT CCTTCTATAA TATTATGGGG TGGAGGGGGG TGGTATGGAG CAAGGGGCAA 
     5341 GTTGGGAAGA CAACCTGTAG GGCCTGCGGG GTCTATTGGG AACCAAGCTG GAGTGCAGTG 
     5401 GCACAATCTT GGCTCACTGC AATCTCCGCC TCCTGGGTTC AAGCGATTCT CCTGCCTCAG 
     5461 CCTCCCGAGT TGTTGGGATT CCAGGCATGC ATGACCAGGC TCAGCTAATT TTTGTTTTTT 
     5521 TGGTAGAGAC GGGGTTTCAC CATATTGGCC AGGCTGGTCT CCAACTCCTA ATCTCAGGTG 
     5581 ATCTACCCAC CTTGGCCTCC CAAATTGCTG GGATTACAGG CGTGAACCAC TGCTCCCTTC 
     5641 CCTGTCCTTC TGATTTTAAA ATAACTATAC CAGCAGGAGG ACGTCCAGAC ACAGCATAGG 
     5701 CTACCTGGCC ATGCCCAACC GGTGGGACAT TTGAGTTGCT TGCTTGGCAC TGTCCTCTCA 
     5761 TGCGTTGGGT CCACTCAGTA GATGCCTGTT GAATTGGGTA CGCGGCCAGC GGCGAGCGGT 
     5821 ATCAGCTCAC TCAAAGGCGG TAATACGGTT ATCCACAGAA TCAGGGGATA ACGCAGGAAA 
     5881 GAACATGTGA GCAAAAGGCC AGCAAAAGGC CAGGAACCGT AAAAAGGCCG CGTTGCTGGC 
     5941 GTTTTTCCAT AGGCTCCGCC CCCCTGACGA GCATCACAAA AATCGACGCT CAAGTCAGAG 
     6001 GTGGCGAAAC CCGACAGGAC TATAAAGATA CCAGGCGTTT CCCCCTGGAA GCTCCCTCGT 
     6061 GCGCTCTCCT GTTCCGACCC TGCCGCTTAC CGGATACCTG TCCGCCTTTC TCCCTTCGGG 
     6121 AAGCGTGGCG CTTTCTCATA GCTCACGCTG TAGGTATCTC AGTTCGGTGT AGGTCGTTCG 
     6181 CTCCAAGCTG GGCTGTGTGC ACGAACCCCC CGTTCAGCCC GACCGCTGCG CCTTATCCGG 
     6241 TAACTATCGT CTTGAGTCCA ACCCGGTAAG ACACGACTTA TCGCCACTGG CAGCAGCCAC 
     6301 TGGTAACAGG ATTAGCAGAG CGAGGTATGT AGGCGGTGCT ACAGAGTTCT TGAAGTGGTG 
     6361 GCCTAACTAC GGCTACACTA GAAGAACAGT ATTTGGTATC TGCGCTCTGC TGAAGCCAGT 
     6421 TACCTTCGGA AAAAGAGTTG GTAGCTCTTG ATCCGGCAAA CAAACCACCG CTGGTAGCGG 
     6481 TGGTTTTTTT GTTTGCAAGC AGCAGATTAC GCGCAGAAAA AAAGGATCTC AAGAAGATCC 
     6541 TTTGATCTTT TCTACGGGGT CTGACGCTCA GTGGAACGAA AACTCACGTT AAGGGATTTT 
     6601 GGTCATGAGA TTATCAAAAA GGATCTTCAC CTAGATCCTT TTAAATTAAA AATGAAGTTT 
     6661 TAAATCAATC TAAAGTATAT ATGAGTAACC TGAGGCTATG GCAGGGCCTG CCGCCCCGAC 
     6721 GTTGGCTGCG AGCCCTGGGC CTTCACCCGA ACTTGGGGGG TGGGGTGGGG AAAAGGAAGA 
     6781 AACGCGGGCG TATTGGCCCC AATGGGGTCT CGGTGGGGTA TCGACAGAGT GCCAGCCCTG 
     6841 GGACCGAACC CCGCGTTTAT GAACAAACGA CCCAACACCG TGCGTTTTAT TCTGTCTTTT 
     6901 TATTGCCGTC ATAGCGCGGG TTCCTTCCGG TATTGTCTCC TTCCGTGTTT CAGTTAGCCT 
     6961 CCCCCTAGGG TGGGCGAAGA ACTCCAGCAT GAGATCCCCG CGCTGGAGGA TCATCCAGCC 
     7021 GGCGTCCCGG AAAACGATTC CGAAGCCCAA CCTTTCATAG AAGGCGGCGG TGGAATCGAA 
     7081 ATCTCGTGAT GGCAGGTTGG GCGTCGCTTG GTCGGTCATT TCGAACCCCA GAGTCCCGCT 
     7141 CAGAAGAACT CGTCAAGAAG GCGATAGAAG GCGATGCGCT GCGAATCGGG AGCGGCGATA 
     7201 CCGTAAAGCA CGAGGAAGCG GTCAGCCCAT TCGCCGCCAA GCTCTTCAGC AATATCACGG 
     7261 GTAGCCAACG CTATGTCCTG ATAGCGATCC GCCACACCCA GCCGGCCACA GTCGATGAAT 
     7321 CCAGAAAAGC GGCCATTTTC CACCATGATA TTCGGCAAGC AGGCATCGCC ATGGGTCACG 
     7381 ACGAGATCCT CGCCGTCGGG CATGCTCGCC TTGAGCCTGG CGAACAGTTC GGCTGGCGCG 
     7441 AGCCCCTGAT GCTCTTCGTC CAGATCATCC TGATCGACAA GACCGGCTTC CATCCGAGTA 
     7501 CGTGCTCGCT CGATGCGATG TTTCGCTTGG TGGTCGAATG GGCAGGTAGC CGGATCAAGC 
     7561 GTATGCAGCC GCCGCATTGC ATCAGCCATG ATGGATACTT TCTCGGCAGG AGCAAGGTGA 
     7621 GATGACAGGA GATCCTGCCC CGGCACTTCG CCCAATAGCA GCCAGTCCCT TCCCGCTTCA 
     7681 GTGACAACGT CGAGCACAGC TGCGCAAGGA ACGCCCGTCG TGGCCAGCCA CGATAGCCGC 
     7741 GCTGCCTCGT CTTGCAGTTC ATTCAGGGCA CCGGACAGGT CGGTCTTGAC AAAAAGAACC 
     7801 GGGCGCCCCT GCGCTGACAG CCGGAACACG GCGGCATCAG AGCAGCCGAT TGTCTGTTGT 
     7861 GCCCAGTCAT AGCCGAATAG CCTCTCCACC CAAGCGGCCG GAGAACCTGC GTGCAATCCA 
     7921 TCTTGTTCAA TCATGCGAAA CGATCCTCAT CCTGTCTCTT GATCGATCTT TGCAAAAGCC 
     7981 TAGGCCTCCA AAAAAGCCTC CTCACTACTT CTGGAATAGC TCAGAGGCCG AGGCGGCCTC 
     8041 GGCCTCTGCA TAAATAAAAA AAATTAGTCA GCCATGGGGC GGAGAATGGG CGGAACTGGG 
     8101 CGGAGTTAGG GGCGGGATGG GCGGAGTTAG GGGCGGGACT ATGGTTGCTG ACTAATTGAG 
     8161 ATGCATGCTT TGCATACTTC TGCCTGCTGG GGAGCCTGGG GACTTTCCAC ACCTGGTTGC 
     8221 TGACTAATTG AGATGCATGC TTTGCATACT TCTGCCTGCT GGGGAGCCTG GGGACTTTCC 
     8281 ACACCCTAAC TGACACACAT TCCACAGCTG GTTCTTTCCG CCTCAGGACT CTTCCTTTTT 
     8341 CAATATTATT GAAGCATTTA TCAGGGTTAT TGTCTCATGA GCGGATACAT ATTTGAATGT 
     8401 ATTTAGAAAA ATAAACAAAT AGGGGTTCCG CGCACATTTC CCCGAAAAGT GCCACCTGAC 
     8461 GCGCCCTGTA GCGGCGCATT AAGCGCGGCG GGTGTGGTGG TTACGCGCAG CGTGACCGCT 
     8521 ACACTTGCCA GCGCCCTAGC GCCCGCTCCT TTCGCTTTCT TCCCTTCCTT TCTCGCCACG 
     8581 TTCGCCGGCT TTCCCCGTCA AGCTCTAAAT CGGGGGCTCC CTTTAGGGTT CCGATTTAGT 
     8641 GCTTTACGGC ACCTCGACCC CAAAAAACTT GATTAGGGTG ATGGTTCACG TAGTGGGCCA 
     8701 TCGCCCTGAT AGACGGTTTT TCGCCCTTTG ACGTTGGAGT CCACGTTCTT TAATAGTGGA 
     8761 CTCTTGTTCC AAACTGGAAC AACACTCAAC CCTATCTCGG TCTATTCTTT TGATTTATAA 
     8821 GGGATTTTGC CGATTTCGGC CTATTGGTTA AAAAATGAGC TGATTTAACA AAAATTTAAC 
     8881 GCGAATTTT
//