LOCUS       RC213956                7935 bp    DNA     circular  SYN 
DEFINITION  ARHGEF18 (Myc-DDK-tagged)-Human Rho/Rac guanine nucleotide exchange factor (GEF) 18 (ARHGEF18), transcript variant 1
ACCESSION   NM_015318
VERSION     NM_015318.3
SOURCE      synthetic construct 
FEATURES    Location/Qualifiers 
     misc_feature    201..924 
                     /label="CMV promoter"  
     misc_feature    839..856 
                     /label="Restriction Enzyme"  
     misc_feature    953..971 
                     /label="T7 promoter"  
     misc_feature    4090..4181 
                     /label="Myc-DDK"  
     misc_feature    4211..4796 
                     /label="PolyA signal"  
     misc_feature    4268..4287 
                     /label="Restriction Enzyme"  
     misc_feature    5072..5744 
                     /label="ColE1"  
     misc_feature    6174..6968 
                     /label="Kan/Neo"  
     misc_feature    7003..7305 
                     /label="SV40 ori"  
     misc_feature    7391..7868 
                     /label="f1 ori"  
     misc_feature    1020..1028 
                     /label="Restriction Enzyme"  
     misc_feature    4065..4072 
                     /label="Restriction Enzyme"  
     misc_feature    1020..4065 
                     /label="ARHGEF18"  
ORIGIN                      
        1 AACAAAATAT TAACGCTTAC AATTTCCATT CGCCATTCAG GCTGCGCAAC TGTTGGGAAG 
       61 GGCGATCGGT GCGGGCCTCT TCGCTATTAC GCCAGCTGGC GAAAGGGGGA TGTGCTGCAA 
      121 GGCGATTAAG TTGGGTAACG CCAGGGTTTT CCCAGTCACG ACGTTGTAAA ACGACGGCCA 
      181 GTGCCAAGCT GATCTATACA TTGAATCAAT ATTGGCAATT AGCCATATTA GTCATTGGTT 
      241 ATATAGCATA AATCAATATT GGCTATTGGC CATTGCATAC GTTGTATCTA TATCATAATA 
      301 TGTACATTTA TATTGGCTCA TGTCCAATAT GACCGCCATG TTGACATTGA TTATTGACTA 
      361 GTTATTAATA GTAATCAATT ACGGGGTCAT TAGTTCATAG CCCATATATG GAGTTCCGCG 
      421 TTACATAACT TACGGTAAAT GGCCCGCCTG GCTGACCGCC CAACGACCCC CGCCCATTGA 
      481 CGTCAATAAT GACGTATGTT CCCATAGTAA CGCCAATAGG GACTTTCCAT TGACGTCAAT 
      541 GGGTGGAGTA TTTACGGTAA ACTGCCCACT TGGCAGTACA TCAAGTGTAT CATATGCCAA 
      601 GTCCGCCCCC TATTGACGTC AATGACGGTA AATGGCCCGC CTGGCATTAT GCCCAGTACA 
      661 TGACCTTACG GGACTTTCCT ACTTGGCAGT ACATCTACGT ATTAGTCATC GCTATTACCA 
      721 TGGTGATGCG GTTTTGGCAG TACACCAATG GGCGTGGATA GCGGTTTGAC TCACGGGGAT 
      781 TTCCAAGTCT CCACCCCATT GACGTCAATG GGAGTTTGTT TTGGCACCAA AATCAACGGG 
      841 ACTTTCCAAA ATGTCGTAAT AACCCCGCCC CGTTGACGCA AATGGGCGGT AGGCGTGTAC 
      901 GGTGGGAGGT CTATATAAGC AGAGCTCGTT TAGTGAACCG TCAGAATTTT GTAATACGAC 
      961 TCACTATAGG GCGGCCGGGA ATTCGTCGAC TGGATCCGGT ACCGAGGAGA TCTGCCGCCG 
     1021 CGATCGCCAT GACGGTCTCT CAGAAAGGGG GTCCCCAGCC AACACCGAGC CCGGCTGGCC 
     1081 CTGGGACGCA ACTCGGACCA ATCACAGGAG AGATGGATGA AGCCGATTCT GCGTTTTTAA 
     1141 AATTTAAGCA GACAGCTGAT GACTCTCTGT CCCTTACATC TCCAAACACC GAGTCCATTT 
     1201 TTGTAGAAGA TCCCTACACC GCCTCGCTGA GGAGTGAGAT TGAGTCAGAC GGCCACGAGT 
     1261 TTGAAGCTGA GTCCTGGAGC CTCGCCGTGG ATGCAGCCTA CGCCAAGAAG CAAAAGAGGG 
     1321 AGGTGGTGAA AAGACAAGAT GTCCTTTATG AGCTGATGCA GACAGAGGTG CACCACGTGC 
     1381 GGACGCTCAA GATCATGCTG AAGGTGTACT CCAGGGCCCT GCAGGAGGAG CTGCAGTTCA 
     1441 GCAGCAAGGC CATTGGCCGC CTCTTCCCAT GCGCTGACGA CCTGCTGGAG ACGCACAGCC 
     1501 ACTTCCTCGC TCGGCTCAAG GAGCGCCGCC AGGAGTCCCT GGAGGAGGGC AGTGACCGGA 
     1561 ATTATGTCAT CCAGAAAATC GGCGACCTCC TGGTTCAGCA GTTTTCAGGT GAAAATGGGG 
     1621 AGAGAATGAA AGAAAAGTAC GGTGTGTTTT GTAGTGGCCA CAATGAAGCT GTTAGTCATT 
     1681 ACAAGTTGCT GCTTCAGCAA AACAAGAAAT TTCAAAACTT GATCAAGAAA ATTGGCAACT 
     1741 TCTCCATCGT GCGGCGGCTT GGCGTGCAGG AGTGCATTCT CCTGGTTACA CAACGCATAA 
     1801 CCAAATACCC AGTGCTGGTG GAGCGCATCA TCCAGAACAC GGAAGCTGGC ACTGAGGACT 
     1861 ATGAAGACCT GACCCAGGCC TTGAACCTCA TCAAAGATAT CATCTCACAA GTGGACGCCA 
     1921 AGGTCAGTGA GTGTGAGAAG GGCCAGCGCC TCAGGGAGAT CGCAGGGAAG ATGGACCTGA 
     1981 AGTCTTCCAG CAAACTCAAG AACGGGCTCA CCTTCCGCAA GGAAGACATG CTTCAGCGGC 
     2041 AGCTCCACCT GGAGGGCATG CTATGCTGGA AGACCACATC AGGGCGCTTG AAAGATATCC 
     2101 TGGCTATCCT GCTGACCGAC GTACTTTTGC TGCTACAAGA AAAAGATCAG AAATACGTCT 
     2161 TTGCTTCTGT GGACTCAAAG CCACCCGTCA TCTCGTTACA AAAGCTCATC GTGAGGGAAG 
     2221 TGGCCAACGA GGAGAAAGCG ATGTTTCTGA TCAGCGCCTC CTTGCAAGGG CCGGAGATGT 
     2281 ATGAAATCTA CACGAGCTCC AAAGAGGACA GGAACGCCTG GATGGCCCAC ATCCAAAGGG 
     2341 CTGTGGAGAG CTGCCCTGAC GAGGAGGAGG GGCCCTTCAG CCTGCCCGAA GAGGAAAGGA 
     2401 AGGTGGTCGA GGCCCGCGCC ACGAGACTCC GGGACTTTCA AGAGCGGTTG AGCATGAAAG 
     2461 ACCAGCTGAT CGCACAGAGC CTCCTAGAGA AACAGCAGAT CTACCTGGAG ATGGCCGAGA 
     2521 TGGGCGGCCT CGAAGACCTG CCCCAGCCCC GAGGCCTATT CCGTGGAGGG GACCCATCCG 
     2581 AGACCCTGCA GGGGGAGCTA ATTCTCAAGT CGGCCATGAG CGAGATCGAG GGCATCCAGA 
     2641 GCCTGATCTG CAGGCAGCTG GGCAGCGCCA ACGGCCAGGC GGAAGACGGA GGCAGCTCCA 
     2701 CAGGCCCGCC CAGGAGGGCT GAGACCTTCG CGGGCTACGA CTGCACAAAC AGCCCCACCA 
     2761 AGAATGGCAG TTTCAAGAAG AAAGTCAGCA GCACTGACCC CAGGCCCCGA GACTGGCGAG 
     2821 GCCCCCCAAA CAGCCCGGAC TTGAAGCTCA GTGACAGTGA CATTCCTGGG AGCTCTGAGG 
     2881 AATCGCCGCA GGTGGTGGAG GCGCCAGGCA CGGAATCCGA TCCCCGTCTG CCCACCGTCC 
     2941 TGGAGTCGGA GCTTGTCCAG CGGATCCAGA CACTGTCCCA GCTGCTCCTG AACCTTCAGG 
     3001 CGGTAATCGC CCACCAGGAC AGCTATGTGG AGACGCAGCG GGCTGCCATC CAGGAGCGGG 
     3061 AGAAGCAGTT CCGGCTGCAG TCGACGCGTG GGAACCTGCT GCTGGAGCAG GAGCGGCAAC 
     3121 GCAACTTCGA GAAGCAGCGG GAGGAGCGCG CGGCCCTGGA GAAGCTGCAG AGCCAGCTGC 
     3181 GGCACGAGCA GCAGCGCTGG GAGCGCGAGC GCCAGTGGCA GCACCAGGAG CTGGAGCGTG 
     3241 CGGGCGCGCG GCTGCAGGAG CGCGAGGGCG AGGCGCGGCA GCTACGCGAG CGGCTGGAGC 
     3301 AGGAGCGGGC CGAGCTGGAG CGCCAGCGCC AGGCCTACCA GCACGACCTG GAGCGGCTGC 
     3361 GCGAGGCCCA GCGTGCCGTG GAGCGCGAGC GGGAGCGCCT GGAGCTGCTG CGCCGCCTCA 
     3421 AGAAGCAGAA CACCGCGCCA GGCGCGCTGC CGCCCGACAC ACTGGCCGAG GCCCAGCCCC 
     3481 CAAGCCACCC TCCCAGCTTC AACGGGGAAG GGCTGGAGGG CCCTCGTGTG AGCATGCTGC 
     3541 CATCCGGCGT GGGGCCAGAG TACGCAGAGC GCCCCGAGGT GGCTCGCCGG GACAGCGCCC 
     3601 CCACCGAGAA CCGGCTGGCC AAGAGCGATG TGCCCATCCA GCTGCTCAGC GCCACCAACC 
     3661 AGTTCCAGAG GCAGGCGGCC GTGCAGCAGC AGATCCCCAC CAAGCTGGCG GCCTCCACCA 
     3721 AGGGTGGCAA GGACAAGGGC GGCAAGAGCA GGGGCTCTCA GCGCTGGGAG AGCTCAGCGT 
     3781 CCTTCGACCT GAAGCAGCAG CTGCTGCTCA ACAAGCTCAT GGGGAAAGAT GAGAGCACCT 
     3841 CACGGAACCG CCGCTCGCTG AGCCCTATCC TGCCCGGCAG ACACAGTCCT GCGCCCCCAC 
     3901 CAGACCCTGG CTTCCCCGCC CCGAGCCCAC CGCCAGCTGA CAGCCCCTCC GAGGGCTTCT 
     3961 CTCTCAAGGC CGGGGGCACA GCCCTCCTGC CCGGGCCCCC AGCTCCCTCG CCACTGCCGG 
     4021 CCACACCACT CAGCGCCAAG GAGGACGCCA GCAAAGAAGA CGTCATCTTC TTCAGCGGAC 
     4081 CGACGCGTAC GCGGCCGCTC GAGCAGAAAC TCATCTCAGA AGAGGATCTG GCAGCAAATG 
     4141 ATATCCTGGA TTACAAGGAT GACGACGATA AGGTTTAAAC GGCCGGCCGC GGTCATAGCT 
     4201 GTTTCCTGAA CAGATCCCGG GTGGCATCCC TGTGACCCCT CCCCAGTGCC TCTCCTGGCC 
     4261 CTGGAAGTTG CCACTCCAGT GCCCACCAGC CTTGTCCTAA TAAAATTAAG TTGCATCATT 
     4321 TTGTCTGACT AGGTGTCCTT CTATAATATT ATGGGGTGGA GGGGGGTGGT ATGGAGCAAG 
     4381 GGGCAAGTTG GGAAGACAAC CTGTAGGGCC TGCGGGGTCT ATTGGGAACC AAGCTGGAGT 
     4441 GCAGTGGCAC AATCTTGGCT CACTGCAATC TCCGCCTCCT GGGTTCAAGC GATTCTCCTG 
     4501 CCTCAGCCTC CCGAGTTGTT GGGATTCCAG GCATGCATGA CCAGGCTCAG CTAATTTTTG 
     4561 TTTTTTTGGT AGAGACGGGG TTTCACCATA TTGGCCAGGC TGGTCTCCAA CTCCTAATCT 
     4621 CAGGTGATCT ACCCACCTTG GCCTCCCAAA TTGCTGGGAT TACAGGCGTG AACCACTGCT 
     4681 CCCTTCCCTG TCCTTCTGAT TTTAAAATAA CTATACCAGC AGGAGGACGT CCAGACACAG 
     4741 CATAGGCTAC CTGGCCATGC CCAACCGGTG GGACATTTGA GTTGCTTGCT TGGCACTGTC 
     4801 CTCTCATGCG TTGGGTCCAC TCAGTAGATG CCTGTTGAAT TGGGTACGCG GCCAGCGGCG 
     4861 AGCGGTATCA GCTCACTCAA AGGCGGTAAT ACGGTTATCC ACAGAATCAG GGGATAACGC 
     4921 AGGAAAGAAC ATGTGAGCAA AAGGCCAGCA AAAGGCCAGG AACCGTAAAA AGGCCGCGTT 
     4981 GCTGGCGTTT TTCCATAGGC TCCGCCCCCC TGACGAGCAT CACAAAAATC GACGCTCAAG 
     5041 TCAGAGGTGG CGAAACCCGA CAGGACTATA AAGATACCAG GCGTTTCCCC CTGGAAGCTC 
     5101 CCTCGTGCGC TCTCCTGTTC CGACCCTGCC GCTTACCGGA TACCTGTCCG CCTTTCTCCC 
     5161 TTCGGGAAGC GTGGCGCTTT CTCATAGCTC ACGCTGTAGG TATCTCAGTT CGGTGTAGGT 
     5221 CGTTCGCTCC AAGCTGGGCT GTGTGCACGA ACCCCCCGTT CAGCCCGACC GCTGCGCCTT 
     5281 ATCCGGTAAC TATCGTCTTG AGTCCAACCC GGTAAGACAC GACTTATCGC CACTGGCAGC 
     5341 AGCCACTGGT AACAGGATTA GCAGAGCGAG GTATGTAGGC GGTGCTACAG AGTTCTTGAA 
     5401 GTGGTGGCCT AACTACGGCT ACACTAGAAG AACAGTATTT GGTATCTGCG CTCTGCTGAA 
     5461 GCCAGTTACC TTCGGAAAAA GAGTTGGTAG CTCTTGATCC GGCAAACAAA CCACCGCTGG 
     5521 TAGCGGTGGT TTTTTTGTTT GCAAGCAGCA GATTACGCGC AGAAAAAAAG GATCTCAAGA 
     5581 AGATCCTTTG ATCTTTTCTA CGGGGTCTGA CGCTCAGTGG AACGAAAACT CACGTTAAGG 
     5641 GATTTTGGTC ATGAGATTAT CAAAAAGGAT CTTCACCTAG ATCCTTTTAA ATTAAAAATG 
     5701 AAGTTTTAAA TCAATCTAAA GTATATATGA GTAACCTGAG GCTATGGCAG GGCCTGCCGC 
     5761 CCCGACGTTG GCTGCGAGCC CTGGGCCTTC ACCCGAACTT GGGGGGTGGG GTGGGGAAAA 
     5821 GGAAGAAACG CGGGCGTATT GGCCCCAATG GGGTCTCGGT GGGGTATCGA CAGAGTGCCA 
     5881 GCCCTGGGAC CGAACCCCGC GTTTATGAAC AAACGACCCA ACACCGTGCG TTTTATTCTG 
     5941 TCTTTTTATT GCCGTCATAG CGCGGGTTCC TTCCGGTATT GTCTCCTTCC GTGTTTCAGT 
     6001 TAGCCTCCCC CTAGGGTGGG CGAAGAACTC CAGCATGAGA TCCCCGCGCT GGAGGATCAT 
     6061 CCAGCCGGCG TCCCGGAAAA CGATTCCGAA GCCCAACCTT TCATAGAAGG CGGCGGTGGA 
     6121 ATCGAAATCT CGTGATGGCA GGTTGGGCGT CGCTTGGTCG GTCATTTCGA ACCCCAGAGT 
     6181 CCCGCTCAGA AGAACTCGTC AAGAAGGCGA TAGAAGGCGA TGCGCTGCGA ATCGGGAGCG 
     6241 GCGATACCGT AAAGCACGAG GAAGCGGTCA GCCCATTCGC CGCCAAGCTC TTCAGCAATA 
     6301 TCACGGGTAG CCAACGCTAT GTCCTGATAG CGATCCGCCA CACCCAGCCG GCCACAGTCG 
     6361 ATGAATCCAG AAAAGCGGCC ATTTTCCACC ATGATATTCG GCAAGCAGGC ATCGCCATGG 
     6421 GTCACGACGA GATCCTCGCC GTCGGGCATG CTCGCCTTGA GCCTGGCGAA CAGTTCGGCT 
     6481 GGCGCGAGCC CCTGATGCTC TTCGTCCAGA TCATCCTGAT CGACAAGACC GGCTTCCATC 
     6541 CGAGTACGTG CTCGCTCGAT GCGATGTTTC GCTTGGTGGT CGAATGGGCA GGTAGCCGGA 
     6601 TCAAGCGTAT GCAGCCGCCG CATTGCATCA GCCATGATGG ATACTTTCTC GGCAGGAGCA 
     6661 AGGTGAGATG ACAGGAGATC CTGCCCCGGC ACTTCGCCCA ATAGCAGCCA GTCCCTTCCC 
     6721 GCTTCAGTGA CAACGTCGAG CACAGCTGCG CAAGGAACGC CCGTCGTGGC CAGCCACGAT 
     6781 AGCCGCGCTG CCTCGTCTTG CAGTTCATTC AGGGCACCGG ACAGGTCGGT CTTGACAAAA 
     6841 AGAACCGGGC GCCCCTGCGC TGACAGCCGG AACACGGCGG CATCAGAGCA GCCGATTGTC 
     6901 TGTTGTGCCC AGTCATAGCC GAATAGCCTC TCCACCCAAG CGGCCGGAGA ACCTGCGTGC 
     6961 AATCCATCTT GTTCAATCAT GCGAAACGAT CCTCATCCTG TCTCTTGATC GATCTTTGCA 
     7021 AAAGCCTAGG CCTCCAAAAA AGCCTCCTCA CTACTTCTGG AATAGCTCAG AGGCCGAGGC 
     7081 GGCCTCGGCC TCTGCATAAA TAAAAAAAAT TAGTCAGCCA TGGGGCGGAG AATGGGCGGA 
     7141 ACTGGGCGGA GTTAGGGGCG GGATGGGCGG AGTTAGGGGC GGGACTATGG TTGCTGACTA 
     7201 ATTGAGATGC ATGCTTTGCA TACTTCTGCC TGCTGGGGAG CCTGGGGACT TTCCACACCT 
     7261 GGTTGCTGAC TAATTGAGAT GCATGCTTTG CATACTTCTG CCTGCTGGGG AGCCTGGGGA 
     7321 CTTTCCACAC CCTAACTGAC ACACATTCCA CAGCTGGTTC TTTCCGCCTC AGGACTCTTC 
     7381 CTTTTTCAAT ATTATTGAAG CATTTATCAG GGTTATTGTC TCATGAGCGG ATACATATTT 
     7441 GAATGTATTT AGAAAAATAA ACAAATAGGG GTTCCGCGCA CATTTCCCCG AAAAGTGCCA 
     7501 CCTGACGCGC CCTGTAGCGG CGCATTAAGC GCGGCGGGTG TGGTGGTTAC GCGCAGCGTG 
     7561 ACCGCTACAC TTGCCAGCGC CCTAGCGCCC GCTCCTTTCG CTTTCTTCCC TTCCTTTCTC 
     7621 GCCACGTTCG CCGGCTTTCC CCGTCAAGCT CTAAATCGGG GGCTCCCTTT AGGGTTCCGA 
     7681 TTTAGTGCTT TACGGCACCT CGACCCCAAA AAACTTGATT AGGGTGATGG TTCACGTAGT 
     7741 GGGCCATCGC CCTGATAGAC GGTTTTTCGC CCTTTGACGT TGGAGTCCAC GTTCTTTAAT 
     7801 AGTGGACTCT TGTTCCAAAC TGGAACAACA CTCAACCCTA TCTCGGTCTA TTCTTTTGAT 
     7861 TTATAAGGGA TTTTGCCGAT TTCGGCCTAT TGGTTAAAAA ATGAGCTGAT TTAACAAAAA 
     7921 TTTAACGCGA ATTTT
//