LOCUS       RC213916                9003 bp    DNA     circular  SYN 
DEFINITION  DROSHA (Myc-DDK-tagged)-Human drosha, ribonuclease type III (DROSHA), transcript variant 1
ACCESSION   NM_013235
VERSION     NM_013235.2
SOURCE      synthetic construct 
FEATURES    Location/Qualifiers 
     misc_feature    201..924 
                     /label="CMV promoter"  
     misc_feature    839..856 
                     /label="Restriction Enzyme"  
     misc_feature    953..971 
                     /label="T7 promoter"  
     misc_feature    5160..5251 
                     /label="Myc-DDK"  
     misc_feature    5281..5866 
                     /label="PolyA signal"  
     misc_feature    5338..5357 
                     /label="Restriction Enzyme"  
     misc_feature    6142..6814 
                     /label="ColE1"  
     misc_feature    7244..8038 
                     /label="Kan/Neo"  
     misc_feature    8073..8375 
                     /label="SV40 ori"  
     misc_feature    8461..8938 
                     /label="f1 ori"  
     misc_feature    1020..1028 
                     /label="Restriction Enzyme"  
     misc_feature    5142..5148 
                     /label="Restriction Enzyme"  
     misc_feature    1020..5142 
                     /label="DROSHA"  
ORIGIN                      
        1 AACAAAATAT TAACGCTTAC AATTTCCATT CGCCATTCAG GCTGCGCAAC TGTTGGGAAG 
       61 GGCGATCGGT GCGGGCCTCT TCGCTATTAC GCCAGCTGGC GAAAGGGGGA TGTGCTGCAA 
      121 GGCGATTAAG TTGGGTAACG CCAGGGTTTT CCCAGTCACG ACGTTGTAAA ACGACGGCCA 
      181 GTGCCAAGCT GATCTATACA TTGAATCAAT ATTGGCAATT AGCCATATTA GTCATTGGTT 
      241 ATATAGCATA AATCAATATT GGCTATTGGC CATTGCATAC GTTGTATCTA TATCATAATA 
      301 TGTACATTTA TATTGGCTCA TGTCCAATAT GACCGCCATG TTGACATTGA TTATTGACTA 
      361 GTTATTAATA GTAATCAATT ACGGGGTCAT TAGTTCATAG CCCATATATG GAGTTCCGCG 
      421 TTACATAACT TACGGTAAAT GGCCCGCCTG GCTGACCGCC CAACGACCCC CGCCCATTGA 
      481 CGTCAATAAT GACGTATGTT CCCATAGTAA CGCCAATAGG GACTTTCCAT TGACGTCAAT 
      541 GGGTGGAGTA TTTACGGTAA ACTGCCCACT TGGCAGTACA TCAAGTGTAT CATATGCCAA 
      601 GTCCGCCCCC TATTGACGTC AATGACGGTA AATGGCCCGC CTGGCATTAT GCCCAGTACA 
      661 TGACCTTACG GGACTTTCCT ACTTGGCAGT ACATCTACGT ATTAGTCATC GCTATTACCA 
      721 TGGTGATGCG GTTTTGGCAG TACACCAATG GGCGTGGATA GCGGTTTGAC TCACGGGGAT 
      781 TTCCAAGTCT CCACCCCATT GACGTCAATG GGAGTTTGTT TTGGCACCAA AATCAACGGG 
      841 ACTTTCCAAA ATGTCGTAAT AACCCCGCCC CGTTGACGCA AATGGGCGGT AGGCGTGTAC 
      901 GGTGGGAGGT CTATATAAGC AGAGCTCGTT TAGTGAACCG TCAGAATTTT GTAATACGAC 
      961 TCACTATAGG GCGGCCGGGA ATTCGTCGAC TGGATCCGGT ACCGAGGAGA TCTGCCGCCG 
     1021 CGATCGCCAT GATGCAGGGA AACACATGTC ACAGAATGTC GTTCCACCCG GGACGAGGGT 
     1081 GTCCCCGAGG ACGAGGAGGA CATGGAGCCA GACCCTCAGC ACCATCCTTT AGGCCCCAAA 
     1141 ATCTGAGGCT GCTTCACCCT CAGCAGCCTC CTGTGCAATA TCAATATGAA CCTCCAAGTG 
     1201 CCCCTTCCAC CACTTTCTCA AACTCTCCAG CCCCCAATTT TCTCCCTCCA CGACCAGACT 
     1261 TTGTACCCTT CCCCCCACCC ATGCCTCCGT CAGCGCAAGG CCCTCTTCCC CCCTGCCCAA 
     1321 TCAGGCCGCC TTTCCCCAAC CACCAGATGA GGCACCCCTT CCCAGTTCCT CCTTGTTTTC 
     1381 CTCCCATGCC ACCACCAATG CCTTGTCCTA ATAACCCCCC AGTCCCTGGG GCACCTCCTG 
     1441 GACAAGGCAC TTTCCCCTTC ATGATGCCCC CTCCCTCCAT GCCTCATCCC CCGCCCCCTC 
     1501 CAGTCATGCC GCAGCAGGTT AATTATCAGT ACCCTCCGGG CTATTCTCAC CACAACTTCC 
     1561 CACCTCCCAG TTTTAATAGT TTCCAGAACA ACCCTAGTTC TTTCCTGCCC AGTGCTAATA 
     1621 ACAGCAGTAG TCCTCATTTC AGACATCTCC CTCCATACCC ACTCCCAAAG GCTCCCAGTG 
     1681 AGAGAAGGTC CCCAGAAAGG CTGAAACACT ATGATGACCA CAGGCACCGA GATCACAGTC 
     1741 ATGGGCGAGG TGAGAGGCAT CGGTCCCTGG ATCGGCGGGA GCGAGGCCGC AGTCCCGACA 
     1801 GGAGAAGACA AGACAGCCGG TACAGATCTG ATTATGACCG AGGGAGAACA CCATCTCGCC 
     1861 ACCGCAGCTA CGAACGGAGC AGAGAGCGAG AACGGGAGAG ACACAGGCAT CGAGACAACC 
     1921 GAAGATCACC ATCTCTGGAA AGGTCCTACA AAAAAGAGTA TAAGAGATCT GGAAGGAGTT 
     1981 ACGGTTTATC GGTTGTTCCT GAACCTGCTG GATGCACACC AGAATTACCT GGGGAGATTA 
     2041 TTAAAAATAC AGATTCTTGG GCCCCACCCC TGGAGATTGT GAATCATCGC TCCCCAAGTA 
     2101 GGGAGAAGAA GAGAGCTCGT TGGGAGGAAG AAAAAGACCG TTGGAGTGAC AACCAGAGTT 
     2161 CTGGCAAAGA CAAGAACTAT ACCTCAATCA AGGAAAAAGA GCCCGAGGAG ACCATGCCTG 
     2221 ACAAGAATGA GGAGGAAGAA GAAGAACTTC TTAAGCCTGT GTGGATTCGA TGCACTCATT 
     2281 CAGAAAACTA CTACTCCAGT GACCCCATGG ATCAGGTGGG AGATTCTACA GTGGTTGGAA 
     2341 CGAGTAGGCT TCGTGACTTA TATGACAAAT TTGAGGAGGA GTTGGGGAGC AGGCAAGAAA 
     2401 AGGCCAAAGC TGCTCGGCCT CCGTGGGAAC CTCCAAAGAC GAAGCTCGAT GAAGATTTAG 
     2461 AGAGTTCCAG TGAATCCGAG TGTGAGTCTG ATGAGGACAG CACCTGTTCT AGCAGCTCAG 
     2521 ACTCTGAAGT TTTTGACGTT ATTGCAGAAA TCAAACGCAA AAAGGCCCAC CCTGACCGAC 
     2581 TTCATGATGA ACTTTGGTAC AACGATCCAG GCCAGATGAA TGATGGACCA CTCTGCAAAT 
     2641 GCAGCGCAAA GGCAAGACGC ACAGGAATTA GGCACAGCAT TTATCCTGGA GAAGAGGCCA 
     2701 TCAAGCCCTG TCGTCCTATG ACCAACAATG CTGGCAGACT TTTCCACTAC CGGATCACAG 
     2761 TCTCCCCGCC TACGAACTTT TTAACTGACA GGCCAACTGT TATAGAATAC GATGATCACG 
     2821 AGTATATCTT TGAAGGATTT TCTATGTTTG CACATGCCCC CCTGACCAAT ATTCCACTGT 
     2881 GTAAAGTAAT TAGATTCAAC ATAGACTACA CGATTCATTT CATTGAAGAG ATGATGCCGG 
     2941 AGAATTTTTG TGTGAAAGGG CTTGAACTCT TTTCACTGTT CCTATTCAGA GATATTTTGG 
     3001 AATTATATGA CTGGAATCTT AAAGGTCCTT TGTTTGAAGA CAGCCCTCCC TGCTGCCCAA 
     3061 GATTTCATTT CATGCCACGT TTTGTAAGAT TTCTTCCAGA TGGAGGAAAG GAAGTGCTGT 
     3121 CCATGCACCA GATTCTCCTG TACTTGTTAA GGTGCAGCAA AGCCCTGGTG CCTGAGGAGG 
     3181 AGATTGCCAA TATGCTTCAG TGGGAGGAGC TGGAGTGGCA GAAATATGCA GAAGAATGCA 
     3241 AAGGCATGAT TGTTACCAAC CCTGGGACGA AACCAAGCTC TGTCCGTATC GATCAACTGG 
     3301 ATCGTGAACA GTTCAACCCC GATGTGATTA CTTTTCCGAT TATCGTCCAC TTTGGGATAC 
     3361 GCCCTGCACA GTTGAGTTAT GCAGGAGACC CACAGTACCA AAAACTGTGG AAGAGTTATG 
     3421 TGAAACTTCG CCACCTCCTA GCAAATAGTC CCAAAGTCAA ACAAACTGAC AAACAGAAGC 
     3481 TGGCACAGAG GGAGGAAGCC CTCCAAAAAA TACGGCAGAA GAATACAATG AGACGAGAAG 
     3541 TAACGGTGGA GCTAAGTAGC CAAGGATTCT GGAAAACTGG CATCCGTTCT GATGTCTGTC 
     3601 AGCATGCAAT GATGCTACCT GTTCTGACCC ATCATATCCG CTACCACCAA TGCCTAATGC 
     3661 ATTTGGACAA GTTGATAGGA TATACTTTCC AAGATCGTTG TCTGTTGCAG CTGGCCATGA 
     3721 CTCATCCAAG TCATCATTTA AATTTTGGAA TGAATCCTGA TCATGCCAGG AATTCATTAT 
     3781 CTAACTGTGG AATTCGGCAG CCCAAATACG GAGACAGAAA AGTTCATCAC ATGCACATGC 
     3841 GGAAGAAAGG GATTAACACC TTGATAAATA TCATGTCACG CCTTGGCCAA GATGACCCAA 
     3901 CTCCCTCGAG GATTAACCAC AATGAACGGT TGGAATTCCT GGGTGATGCT GTTGTTGAAT 
     3961 TTCTGACCAG CGTCCATTTG TACTATTTGT TTCCTAGTCT GGAAGAAGGA GGATTAGCAA 
     4021 CCTATCGGAC TGCCATTGTT CAGAATCAGC ACCTTGCCAT GCTAGCAAAG AAACTTGAAC 
     4081 TGGATCCATT TATGCTGTAT GCTCACGGGC CTGACCTTTG TAGAGAATCG GACCTTCGAC 
     4141 ATGCAATGGC CAATTGTTTT GAAGCGTTAA TAGGAGCTGT TTACTTGGAG GGAAGCCTGG 
     4201 AGGAAGCCAA GCAGTTATTT GGACGCTTGC TCTTTAATGA TCCGGACCTG CGCGAAGTCT 
     4261 GGCTCAATTA TCCTCTCCAC CCACTCCAAC TACAAGAGCC AAATACTGAT CGACAACTTA 
     4321 TTGAAACTTC TCCAGTTCTA CAAAAACTTA CTGAGTTTGA AGAAGCAATT GGAGTAATTT 
     4381 TTACTCATGT TCGACTTCTG GCAAGGGCAT TCACATTGAG AACTGTGGGA TTTAACCATC 
     4441 TGACCCTAGG CCACAATCAG AGAATGGAAT TCCTAGGTGA CTCCATAATG CAACTGGTAG 
     4501 CCACAGAGTA CTTATTCATT CATTTCCCAG ATCATCATGA AGGACACTTA ACTTTGTTGC 
     4561 GAAGCTCTTT GGTGAATAAT AGAACTCAGG CCAAGGTAGC GGAGGAGCTG GGCATGCAGG 
     4621 AGTACGCCAT AACCAACGAC AAGACCAAGA GGCCTGTGGC GCTTCGCACC AAGACCTTGG 
     4681 CGGACCTTTT GGAATCATTT ATTGCAGCGC TGTACACTGA TAAGGATTTG GAATATGTTC 
     4741 ATACTTTCAT GAATGTCTGC TTCTTTCCAC GATTGAAAGA ATTCATTTTG AATCAGGATT 
     4801 GGAATGACCC CAAATCCCAG CTTCAGCAGT GTTGCTTGAC ACTTAGGACA GAAGGAAAAG 
     4861 AGCCAGACAT TCCTCTGTAC AAGACTCTGC AGACAGTGGG CCCATCCCAT GCCCGAACCT 
     4921 ACACTGTGGC TGTTTATTTC AAGGGAGAAA GAATAGGCTG TGGGAAAGGA CCAAGTATTC 
     4981 AGCAAGCGGA AATGGGAGCA GCAATGGATG CGCTTGAAAA ATATAATTTT CCCCAGATGG 
     5041 CCCATCAGAA GCGGTTCATC GAACGGAAGT ACAGACAAGA GTTAAAAGAA ATGAGGTGGG 
     5101 AAAGAGAGCA TCAAGAGAGA GAGCCAGATG AGACTGAAGA CATCAAGAAA ACGCGTACGC 
     5161 GGCCGCTCGA GCAGAAACTC ATCTCAGAAG AGGATCTGGC AGCAAATGAT ATCCTGGATT 
     5221 ACAAGGATGA CGACGATAAG GTTTAAACGG CCGGCCGCGG TCATAGCTGT TTCCTGAACA 
     5281 GATCCCGGGT GGCATCCCTG TGACCCCTCC CCAGTGCCTC TCCTGGCCCT GGAAGTTGCC 
     5341 ACTCCAGTGC CCACCAGCCT TGTCCTAATA AAATTAAGTT GCATCATTTT GTCTGACTAG 
     5401 GTGTCCTTCT ATAATATTAT GGGGTGGAGG GGGGTGGTAT GGAGCAAGGG GCAAGTTGGG 
     5461 AAGACAACCT GTAGGGCCTG CGGGGTCTAT TGGGAACCAA GCTGGAGTGC AGTGGCACAA 
     5521 TCTTGGCTCA CTGCAATCTC CGCCTCCTGG GTTCAAGCGA TTCTCCTGCC TCAGCCTCCC 
     5581 GAGTTGTTGG GATTCCAGGC ATGCATGACC AGGCTCAGCT AATTTTTGTT TTTTTGGTAG 
     5641 AGACGGGGTT TCACCATATT GGCCAGGCTG GTCTCCAACT CCTAATCTCA GGTGATCTAC 
     5701 CCACCTTGGC CTCCCAAATT GCTGGGATTA CAGGCGTGAA CCACTGCTCC CTTCCCTGTC 
     5761 CTTCTGATTT TAAAATAACT ATACCAGCAG GAGGACGTCC AGACACAGCA TAGGCTACCT 
     5821 GGCCATGCCC AACCGGTGGG ACATTTGAGT TGCTTGCTTG GCACTGTCCT CTCATGCGTT 
     5881 GGGTCCACTC AGTAGATGCC TGTTGAATTG GGTACGCGGC CAGCGGCGAG CGGTATCAGC 
     5941 TCACTCAAAG GCGGTAATAC GGTTATCCAC AGAATCAGGG GATAACGCAG GAAAGAACAT 
     6001 GTGAGCAAAA GGCCAGCAAA AGGCCAGGAA CCGTAAAAAG GCCGCGTTGC TGGCGTTTTT 
     6061 CCATAGGCTC CGCCCCCCTG ACGAGCATCA CAAAAATCGA CGCTCAAGTC AGAGGTGGCG 
     6121 AAACCCGACA GGACTATAAA GATACCAGGC GTTTCCCCCT GGAAGCTCCC TCGTGCGCTC 
     6181 TCCTGTTCCG ACCCTGCCGC TTACCGGATA CCTGTCCGCC TTTCTCCCTT CGGGAAGCGT 
     6241 GGCGCTTTCT CATAGCTCAC GCTGTAGGTA TCTCAGTTCG GTGTAGGTCG TTCGCTCCAA 
     6301 GCTGGGCTGT GTGCACGAAC CCCCCGTTCA GCCCGACCGC TGCGCCTTAT CCGGTAACTA 
     6361 TCGTCTTGAG TCCAACCCGG TAAGACACGA CTTATCGCCA CTGGCAGCAG CCACTGGTAA 
     6421 CAGGATTAGC AGAGCGAGGT ATGTAGGCGG TGCTACAGAG TTCTTGAAGT GGTGGCCTAA 
     6481 CTACGGCTAC ACTAGAAGAA CAGTATTTGG TATCTGCGCT CTGCTGAAGC CAGTTACCTT 
     6541 CGGAAAAAGA GTTGGTAGCT CTTGATCCGG CAAACAAACC ACCGCTGGTA GCGGTGGTTT 
     6601 TTTTGTTTGC AAGCAGCAGA TTACGCGCAG AAAAAAAGGA TCTCAAGAAG ATCCTTTGAT 
     6661 CTTTTCTACG GGGTCTGACG CTCAGTGGAA CGAAAACTCA CGTTAAGGGA TTTTGGTCAT 
     6721 GAGATTATCA AAAAGGATCT TCACCTAGAT CCTTTTAAAT TAAAAATGAA GTTTTAAATC 
     6781 AATCTAAAGT ATATATGAGT AACCTGAGGC TATGGCAGGG CCTGCCGCCC CGACGTTGGC 
     6841 TGCGAGCCCT GGGCCTTCAC CCGAACTTGG GGGGTGGGGT GGGGAAAAGG AAGAAACGCG 
     6901 GGCGTATTGG CCCCAATGGG GTCTCGGTGG GGTATCGACA GAGTGCCAGC CCTGGGACCG 
     6961 AACCCCGCGT TTATGAACAA ACGACCCAAC ACCGTGCGTT TTATTCTGTC TTTTTATTGC 
     7021 CGTCATAGCG CGGGTTCCTT CCGGTATTGT CTCCTTCCGT GTTTCAGTTA GCCTCCCCCT 
     7081 AGGGTGGGCG AAGAACTCCA GCATGAGATC CCCGCGCTGG AGGATCATCC AGCCGGCGTC 
     7141 CCGGAAAACG ATTCCGAAGC CCAACCTTTC ATAGAAGGCG GCGGTGGAAT CGAAATCTCG 
     7201 TGATGGCAGG TTGGGCGTCG CTTGGTCGGT CATTTCGAAC CCCAGAGTCC CGCTCAGAAG 
     7261 AACTCGTCAA GAAGGCGATA GAAGGCGATG CGCTGCGAAT CGGGAGCGGC GATACCGTAA 
     7321 AGCACGAGGA AGCGGTCAGC CCATTCGCCG CCAAGCTCTT CAGCAATATC ACGGGTAGCC 
     7381 AACGCTATGT CCTGATAGCG ATCCGCCACA CCCAGCCGGC CACAGTCGAT GAATCCAGAA 
     7441 AAGCGGCCAT TTTCCACCAT GATATTCGGC AAGCAGGCAT CGCCATGGGT CACGACGAGA 
     7501 TCCTCGCCGT CGGGCATGCT CGCCTTGAGC CTGGCGAACA GTTCGGCTGG CGCGAGCCCC 
     7561 TGATGCTCTT CGTCCAGATC ATCCTGATCG ACAAGACCGG CTTCCATCCG AGTACGTGCT 
     7621 CGCTCGATGC GATGTTTCGC TTGGTGGTCG AATGGGCAGG TAGCCGGATC AAGCGTATGC 
     7681 AGCCGCCGCA TTGCATCAGC CATGATGGAT ACTTTCTCGG CAGGAGCAAG GTGAGATGAC 
     7741 AGGAGATCCT GCCCCGGCAC TTCGCCCAAT AGCAGCCAGT CCCTTCCCGC TTCAGTGACA 
     7801 ACGTCGAGCA CAGCTGCGCA AGGAACGCCC GTCGTGGCCA GCCACGATAG CCGCGCTGCC 
     7861 TCGTCTTGCA GTTCATTCAG GGCACCGGAC AGGTCGGTCT TGACAAAAAG AACCGGGCGC 
     7921 CCCTGCGCTG ACAGCCGGAA CACGGCGGCA TCAGAGCAGC CGATTGTCTG TTGTGCCCAG 
     7981 TCATAGCCGA ATAGCCTCTC CACCCAAGCG GCCGGAGAAC CTGCGTGCAA TCCATCTTGT 
     8041 TCAATCATGC GAAACGATCC TCATCCTGTC TCTTGATCGA TCTTTGCAAA AGCCTAGGCC 
     8101 TCCAAAAAAG CCTCCTCACT ACTTCTGGAA TAGCTCAGAG GCCGAGGCGG CCTCGGCCTC 
     8161 TGCATAAATA AAAAAAATTA GTCAGCCATG GGGCGGAGAA TGGGCGGAAC TGGGCGGAGT 
     8221 TAGGGGCGGG ATGGGCGGAG TTAGGGGCGG GACTATGGTT GCTGACTAAT TGAGATGCAT 
     8281 GCTTTGCATA CTTCTGCCTG CTGGGGAGCC TGGGGACTTT CCACACCTGG TTGCTGACTA 
     8341 ATTGAGATGC ATGCTTTGCA TACTTCTGCC TGCTGGGGAG CCTGGGGACT TTCCACACCC 
     8401 TAACTGACAC ACATTCCACA GCTGGTTCTT TCCGCCTCAG GACTCTTCCT TTTTCAATAT 
     8461 TATTGAAGCA TTTATCAGGG TTATTGTCTC ATGAGCGGAT ACATATTTGA ATGTATTTAG 
     8521 AAAAATAAAC AAATAGGGGT TCCGCGCACA TTTCCCCGAA AAGTGCCACC TGACGCGCCC 
     8581 TGTAGCGGCG CATTAAGCGC GGCGGGTGTG GTGGTTACGC GCAGCGTGAC CGCTACACTT 
     8641 GCCAGCGCCC TAGCGCCCGC TCCTTTCGCT TTCTTCCCTT CCTTTCTCGC CACGTTCGCC 
     8701 GGCTTTCCCC GTCAAGCTCT AAATCGGGGG CTCCCTTTAG GGTTCCGATT TAGTGCTTTA 
     8761 CGGCACCTCG ACCCCAAAAA ACTTGATTAG GGTGATGGTT CACGTAGTGG GCCATCGCCC 
     8821 TGATAGACGG TTTTTCGCCC TTTGACGTTG GAGTCCACGT TCTTTAATAG TGGACTCTTG 
     8881 TTCCAAACTG GAACAACACT CAACCCTATC TCGGTCTATT CTTTTGATTT ATAAGGGATT 
     8941 TTGCCGATTT CGGCCTATTG GTTAAAAAAT GAGCTGATTT AACAAAAATT TAACGCGAAT 
     9001 TTT
//