LOCUS       RC213888                8391 bp    DNA     circular  SYN 
DEFINITION  ATP2B4 (Myc-DDK-tagged)-Human ATPase, Ca++ transporting, plasma membrane 4 (ATP2B4), transcript variant 1
ACCESSION   NM_001001396
VERSION     NM_001001396.2
SOURCE      synthetic construct 
FEATURES    Location/Qualifiers 
     misc_feature    201..924 
                     /label="CMV promoter"  
     misc_feature    839..856 
                     /label="Restriction Enzyme"  
     misc_feature    953..971 
                     /label="T7 promoter"  
     misc_feature    4548..4639 
                     /label="Myc-DDK"  
     misc_feature    4669..5254 
                     /label="PolyA signal"  
     misc_feature    4726..4745 
                     /label="Restriction Enzyme"  
     misc_feature    5530..6202 
                     /label="ColE1"  
     misc_feature    6632..7426 
                     /label="Kan/Neo"  
     misc_feature    7461..7763 
                     /label="SV40 ori"  
     misc_feature    7849..8326 
                     /label="f1 ori"  
     misc_feature    1020..1028 
                     /label="Restriction Enzyme"  
     misc_feature    4530..4536 
                     /label="Restriction Enzyme"  
     misc_feature    1020..4530 
                     /label="ATP2B4"  
ORIGIN                      
        1 AACAAAATAT TAACGCTTAC AATTTCCATT CGCCATTCAG GCTGCGCAAC TGTTGGGAAG 
       61 GGCGATCGGT GCGGGCCTCT TCGCTATTAC GCCAGCTGGC GAAAGGGGGA TGTGCTGCAA 
      121 GGCGATTAAG TTGGGTAACG CCAGGGTTTT CCCAGTCACG ACGTTGTAAA ACGACGGCCA 
      181 GTGCCAAGCT GATCTATACA TTGAATCAAT ATTGGCAATT AGCCATATTA GTCATTGGTT 
      241 ATATAGCATA AATCAATATT GGCTATTGGC CATTGCATAC GTTGTATCTA TATCATAATA 
      301 TGTACATTTA TATTGGCTCA TGTCCAATAT GACCGCCATG TTGACATTGA TTATTGACTA 
      361 GTTATTAATA GTAATCAATT ACGGGGTCAT TAGTTCATAG CCCATATATG GAGTTCCGCG 
      421 TTACATAACT TACGGTAAAT GGCCCGCCTG GCTGACCGCC CAACGACCCC CGCCCATTGA 
      481 CGTCAATAAT GACGTATGTT CCCATAGTAA CGCCAATAGG GACTTTCCAT TGACGTCAAT 
      541 GGGTGGAGTA TTTACGGTAA ACTGCCCACT TGGCAGTACA TCAAGTGTAT CATATGCCAA 
      601 GTCCGCCCCC TATTGACGTC AATGACGGTA AATGGCCCGC CTGGCATTAT GCCCAGTACA 
      661 TGACCTTACG GGACTTTCCT ACTTGGCAGT ACATCTACGT ATTAGTCATC GCTATTACCA 
      721 TGGTGATGCG GTTTTGGCAG TACACCAATG GGCGTGGATA GCGGTTTGAC TCACGGGGAT 
      781 TTCCAAGTCT CCACCCCATT GACGTCAATG GGAGTTTGTT TTGGCACCAA AATCAACGGG 
      841 ACTTTCCAAA ATGTCGTAAT AACCCCGCCC CGTTGACGCA AATGGGCGGT AGGCGTGTAC 
      901 GGTGGGAGGT CTATATAAGC AGAGCTCGTT TAGTGAACCG TCAGAATTTT GTAATACGAC 
      961 TCACTATAGG GCGGCCGGGA ATTCGTCGAC TGGATCCGGT ACCGAGGAGA TCTGCCGCCG 
     1021 CGATCGCCAT GACGAACCCA TCAGACCGTG TCTTGCCTGC CAACTCGATG GCCGAGAGCC 
     1081 GTGAAGGGGA CTTTGGCTGC ACAGTAATGG AACTGAGGAA GCTCATGGAG CTGCGTTCAA 
     1141 GGGATGCACT GACCCAGATT AATGTCCACT ATGGAGGTGT ACAGAATCTC TGCAGTAGAC 
     1201 TGAAAACCTC CCCTGTGGAA GGTCTGTCTG GGAACCCTGC AGATCTGGAG AAACGTAGGC 
     1261 AGGTGTTTGG ACACAACGTG ATCCCCCCCA AAAAGCCCAA GACTTTCTTA GAATTAGTGT 
     1321 GGGAAGCTCT TCAAGATGTC ACGCTTATCA TCCTGGAGAT TGCAGCCATC ATCTCCCTGG 
     1381 TCCTGTCCTT TTATCGCCCT GCTGGTGAAG AAAATGAACT GTGTGGTCAA GTCGCAACTA 
     1441 CCCCAGAAGA TGAAAATGAG GCACAAGCTG GCTGGATTGA GGGGGCAGCC ATCCTTTTCT 
     1501 CAGTGATCAT CGTGGTGTTA GTGACTGCCT TTAATGATTG GAGCAAAGAG AAGCAATTCC 
     1561 GGGGGCTGCA GTGCCGCATT GAACAGGAGC AAAAGTTCTC CATCATCCGA AACGGTCAAC 
     1621 TCATCCAGCT CCCTGTGGCT GAGATTGTGG TTGGTGATAT TGCCCAAGTC AAATACGGTG 
     1681 ATCTGCTGCC TGCAGATGGA ATCCTGATCC AAGGGAATGA TCTGAAGATT GATGAGAGCT 
     1741 CTCTGACAGG GGAATCTGAC CATGTCAAGA AGTCCCTGGA CAAAGACCCC ATGTTGCTCT 
     1801 CAGGGACCCA TGTCATGGAA GGTTCTGGCC GGATGGTGGT GACAGCTGTT GGTGTCAACT 
     1861 CTCAGACTGG AATCATCCTT ACTCTCTTGG GGGTCAATGA GGATGACGAA GGGGAGAAAA 
     1921 AGAAGAAAGG TAAAAAACAA GGAGTCCCTG AAAATCGCAA CAAAGCAAAG ACCCAAGACG 
     1981 GAGTGGCCCT GGAAATCCAG CCACTCAACA GCCAGGAGGG AATCGACAAT GAGGAAAAGG 
     2041 ACAAGAAGGC AGTCAAGGTG CCTAAAAAGG AGAAGTCAGT GCTGCAGGGC AAGCTGACTC 
     2101 GCCTGGCTGT TCAGATTGGG AAAGCCGGTC TGCTCATGTC TGCTCTCACG GTTTTCATCC 
     2161 TGATTCTATA CTTTGTGATT GACAACTTTG TGATAAATCG CAGACCATGG CTCCCTGAGT 
     2221 GTACTCCCAT CTACATCCAG TACTTTGTCA AGTTCTTCAT CATCGGCATC ACTGTACTGG 
     2281 TGGTGGCTGT GCCAGAGGGG CTGCCTCTGG CTGTCACCAT CTCACTGGCC TACTCTGTGA 
     2341 AGAAAATGAT GAAAGACAAT AACCTAGTAC GGCACTTGGA TGCTTGTGAG ACCATGGGCA 
     2401 ACGCCACCGC CATCTGCTCT GATAAGACAG GCACGTTGAC CATGAACCGC ATGACTGTGG 
     2461 TACAAGCTTA TATTGGGGGC ATCCATTACC GTCAAATCCC AAGCCCTGAT GTCTTCCTGC 
     2521 CCAAAGTCCT GGACCTCATT GTCAATGGCA TTTCTATCAA CAGTGCTTAT ACCTCCAAGA 
     2581 TTCTGCCTCC AGAGAAGGAG GGAGGCCTGC CTCGGCAGGT GGGCAACAAG ACCGAGTGTG 
     2641 CTCTGCTAGG CTTTGTCACA GATCTGAAGC AGGATTATCA GGCTGTGCGT AATGAAGTGC 
     2701 CCGAGGAGAA GCTCTACAAG GTGTACACCT TTAACTCAGT GCGCAAGTCA ATGAGCACCG 
     2761 TCATCAGGAA TCCCAACGGT GGCTTCCGTA TGTACAGCAA GGGCGCCTCT GAGATCATCT 
     2821 TGCGCAAGTG TAATCGAATC CTGGACCGGA AAGGGGAAGC AGTGCCATTC AAGAATAAAG 
     2881 ACAGAGATGA TATGGTACGC ACTGTCATCG AGCCCATGGC CTGTGATGGA CTCCGGACTA 
     2941 TCTGCATAGC TTACCGGGAC TTCGATGACA CAGAGCCCTC TTGGGACAAT GAGAATGAGA 
     3001 TCCTCACCGA ACTGACCTGT ATCGCGGTGG TGGGCATTGA GGACCCTGTG CGCCCAGAGG 
     3061 TGCCAGATGC TATTGCCAAA TGCAAACAAG CTGGCATTAC TGTCAGAATG GTGACAGGTG 
     3121 ACAACATCAA CACAGCCCGG GCCATTGCCA CCAAATGTGG CATTCTGACA CCTGGGGATG 
     3181 ACTTCCTGTG CTTAGAAGGC AAAGAATTCA ACCGGCTCAT CCGCAACGAG AAAGGCGAGG 
     3241 TAGAGCAAGA AAAGCTGGAC AAGATCTGGC CTAAGCTTCG GGTCCTGGCG CGATCTTCTC 
     3301 CCACTGACAA GCACACCCTG GTGAAAGGCA TAATTGACAG CACTGTTGGG GAACACCGGC 
     3361 AGGTCGTGGC TGTCACTGGT GATGGCACAA ATGACGGGCC TGCTCTGAAG AAAGCGGATG 
     3421 TTGGTTTTGC CATGGGCATC GCAGGCACAG ATGTAGCAAA GGAGGCTTCA GACATCATCC 
     3481 TAACAGATGA CAACTTCACC AGCATTGTGA AGGCAGTGAT GTGGGGACGA AATGTCTATG 
     3541 ACAGCATCTC CAAGTTCCTG CAGTTCCAGC TCACTGTCAA TGTGGTGGCC GTGATTGTAG 
     3601 CCTTCACTGG AGCCTGTATC ACTCAGGATT CCCCATTGAA AGCTGTGCAG ATGTTGTGGG 
     3661 TTAATCTGAT CATGGACACT TTTGCTTCAT TGGCCCTGGC CACAGAGCCC CCTACGGAAT 
     3721 CTCTGTTGAA GCGGCGCCCC TATGGCCGAA ATAAGCCTCT GATCTCACGC ACTATGATGA 
     3781 AGAACATCTT GGGCCATGCA TTCTATCAGC TCATTGTCAT CTTTATCCTT GTCTTTGCGG 
     3841 GTGAGAAATT CTTTGATATT GATAGTGGGA GGAAGGCACC TCTACATTCA CCACCCAGCC 
     3901 AGCACTATAC CATTGTTTTT AACACCTTCG TGCTGATGCA GCTCTTCAAT GAAATCAACT 
     3961 CCCGAAAGAT CCATGGAGAG AAGAACGTCT TTTCAGGCAT CTACCGCAAC ATTATCTTCT 
     4021 GCTCTGTAGT CTTGGGCACA TTCATCTGCC AGATTTTCAT CGTGGAATTT GGGGGTAAAC 
     4081 CCTTCAGTTG TACAAGCCTC AGCCTGTCTC AGTGGCTGTG GTGTCTCTTC ATTGGGATTG 
     4141 GAGAACTTCT GTGGGGCCAG TTCATCTCCG CAATACCTAC CCGATCCCTG AAGTTCCTGA 
     4201 AGGAGGCTGG GCATGGCACC ACCAAAGAGG AGATCACCAA GGATGCCGAG GGACTGGATG 
     4261 AGATTGACCA TGCTGAGATG GAGCTGCGCC GAGGCCAGAT CCTCTGGTTC CGGGGCCTGA 
     4321 ACCGTATCCA GACTCAGATC GACGTAATTA ACACATTCCA GACGGGAGCC TCTTTTAAGG 
     4381 GAGTCCTAAG GCGACAGAAC ATGGGTCAAC ACCTTGATGT AAAACTTGTT CCTAGTTCAT 
     4441 CCTATGTAGC AGTTGCACCA GTCAAATCTT CCCCCACCAC TTCTGTTCCT GCTGTTTCAT 
     4501 CTCCTCCTAT GGGCAATCAA AGTGGTCAAA GCGTTCCAAC GCGTACGCGG CCGCTCGAGC 
     4561 AGAAACTCAT CTCAGAAGAG GATCTGGCAG CAAATGATAT CCTGGATTAC AAGGATGACG 
     4621 ACGATAAGGT TTAAACGGCC GGCCGCGGTC ATAGCTGTTT CCTGAACAGA TCCCGGGTGG 
     4681 CATCCCTGTG ACCCCTCCCC AGTGCCTCTC CTGGCCCTGG AAGTTGCCAC TCCAGTGCCC 
     4741 ACCAGCCTTG TCCTAATAAA ATTAAGTTGC ATCATTTTGT CTGACTAGGT GTCCTTCTAT 
     4801 AATATTATGG GGTGGAGGGG GGTGGTATGG AGCAAGGGGC AAGTTGGGAA GACAACCTGT 
     4861 AGGGCCTGCG GGGTCTATTG GGAACCAAGC TGGAGTGCAG TGGCACAATC TTGGCTCACT 
     4921 GCAATCTCCG CCTCCTGGGT TCAAGCGATT CTCCTGCCTC AGCCTCCCGA GTTGTTGGGA 
     4981 TTCCAGGCAT GCATGACCAG GCTCAGCTAA TTTTTGTTTT TTTGGTAGAG ACGGGGTTTC 
     5041 ACCATATTGG CCAGGCTGGT CTCCAACTCC TAATCTCAGG TGATCTACCC ACCTTGGCCT 
     5101 CCCAAATTGC TGGGATTACA GGCGTGAACC ACTGCTCCCT TCCCTGTCCT TCTGATTTTA 
     5161 AAATAACTAT ACCAGCAGGA GGACGTCCAG ACACAGCATA GGCTACCTGG CCATGCCCAA 
     5221 CCGGTGGGAC ATTTGAGTTG CTTGCTTGGC ACTGTCCTCT CATGCGTTGG GTCCACTCAG 
     5281 TAGATGCCTG TTGAATTGGG TACGCGGCCA GCGGCGAGCG GTATCAGCTC ACTCAAAGGC 
     5341 GGTAATACGG TTATCCACAG AATCAGGGGA TAACGCAGGA AAGAACATGT GAGCAAAAGG 
     5401 CCAGCAAAAG GCCAGGAACC GTAAAAAGGC CGCGTTGCTG GCGTTTTTCC ATAGGCTCCG 
     5461 CCCCCCTGAC GAGCATCACA AAAATCGACG CTCAAGTCAG AGGTGGCGAA ACCCGACAGG 
     5521 ACTATAAAGA TACCAGGCGT TTCCCCCTGG AAGCTCCCTC GTGCGCTCTC CTGTTCCGAC 
     5581 CCTGCCGCTT ACCGGATACC TGTCCGCCTT TCTCCCTTCG GGAAGCGTGG CGCTTTCTCA 
     5641 TAGCTCACGC TGTAGGTATC TCAGTTCGGT GTAGGTCGTT CGCTCCAAGC TGGGCTGTGT 
     5701 GCACGAACCC CCCGTTCAGC CCGACCGCTG CGCCTTATCC GGTAACTATC GTCTTGAGTC 
     5761 CAACCCGGTA AGACACGACT TATCGCCACT GGCAGCAGCC ACTGGTAACA GGATTAGCAG 
     5821 AGCGAGGTAT GTAGGCGGTG CTACAGAGTT CTTGAAGTGG TGGCCTAACT ACGGCTACAC 
     5881 TAGAAGAACA GTATTTGGTA TCTGCGCTCT GCTGAAGCCA GTTACCTTCG GAAAAAGAGT 
     5941 TGGTAGCTCT TGATCCGGCA AACAAACCAC CGCTGGTAGC GGTGGTTTTT TTGTTTGCAA 
     6001 GCAGCAGATT ACGCGCAGAA AAAAAGGATC TCAAGAAGAT CCTTTGATCT TTTCTACGGG 
     6061 GTCTGACGCT CAGTGGAACG AAAACTCACG TTAAGGGATT TTGGTCATGA GATTATCAAA 
     6121 AAGGATCTTC ACCTAGATCC TTTTAAATTA AAAATGAAGT TTTAAATCAA TCTAAAGTAT 
     6181 ATATGAGTAA CCTGAGGCTA TGGCAGGGCC TGCCGCCCCG ACGTTGGCTG CGAGCCCTGG 
     6241 GCCTTCACCC GAACTTGGGG GGTGGGGTGG GGAAAAGGAA GAAACGCGGG CGTATTGGCC 
     6301 CCAATGGGGT CTCGGTGGGG TATCGACAGA GTGCCAGCCC TGGGACCGAA CCCCGCGTTT 
     6361 ATGAACAAAC GACCCAACAC CGTGCGTTTT ATTCTGTCTT TTTATTGCCG TCATAGCGCG 
     6421 GGTTCCTTCC GGTATTGTCT CCTTCCGTGT TTCAGTTAGC CTCCCCCTAG GGTGGGCGAA 
     6481 GAACTCCAGC ATGAGATCCC CGCGCTGGAG GATCATCCAG CCGGCGTCCC GGAAAACGAT 
     6541 TCCGAAGCCC AACCTTTCAT AGAAGGCGGC GGTGGAATCG AAATCTCGTG ATGGCAGGTT 
     6601 GGGCGTCGCT TGGTCGGTCA TTTCGAACCC CAGAGTCCCG CTCAGAAGAA CTCGTCAAGA 
     6661 AGGCGATAGA AGGCGATGCG CTGCGAATCG GGAGCGGCGA TACCGTAAAG CACGAGGAAG 
     6721 CGGTCAGCCC ATTCGCCGCC AAGCTCTTCA GCAATATCAC GGGTAGCCAA CGCTATGTCC 
     6781 TGATAGCGAT CCGCCACACC CAGCCGGCCA CAGTCGATGA ATCCAGAAAA GCGGCCATTT 
     6841 TCCACCATGA TATTCGGCAA GCAGGCATCG CCATGGGTCA CGACGAGATC CTCGCCGTCG 
     6901 GGCATGCTCG CCTTGAGCCT GGCGAACAGT TCGGCTGGCG CGAGCCCCTG ATGCTCTTCG 
     6961 TCCAGATCAT CCTGATCGAC AAGACCGGCT TCCATCCGAG TACGTGCTCG CTCGATGCGA 
     7021 TGTTTCGCTT GGTGGTCGAA TGGGCAGGTA GCCGGATCAA GCGTATGCAG CCGCCGCATT 
     7081 GCATCAGCCA TGATGGATAC TTTCTCGGCA GGAGCAAGGT GAGATGACAG GAGATCCTGC 
     7141 CCCGGCACTT CGCCCAATAG CAGCCAGTCC CTTCCCGCTT CAGTGACAAC GTCGAGCACA 
     7201 GCTGCGCAAG GAACGCCCGT CGTGGCCAGC CACGATAGCC GCGCTGCCTC GTCTTGCAGT 
     7261 TCATTCAGGG CACCGGACAG GTCGGTCTTG ACAAAAAGAA CCGGGCGCCC CTGCGCTGAC 
     7321 AGCCGGAACA CGGCGGCATC AGAGCAGCCG ATTGTCTGTT GTGCCCAGTC ATAGCCGAAT 
     7381 AGCCTCTCCA CCCAAGCGGC CGGAGAACCT GCGTGCAATC CATCTTGTTC AATCATGCGA 
     7441 AACGATCCTC ATCCTGTCTC TTGATCGATC TTTGCAAAAG CCTAGGCCTC CAAAAAAGCC 
     7501 TCCTCACTAC TTCTGGAATA GCTCAGAGGC CGAGGCGGCC TCGGCCTCTG CATAAATAAA 
     7561 AAAAATTAGT CAGCCATGGG GCGGAGAATG GGCGGAACTG GGCGGAGTTA GGGGCGGGAT 
     7621 GGGCGGAGTT AGGGGCGGGA CTATGGTTGC TGACTAATTG AGATGCATGC TTTGCATACT 
     7681 TCTGCCTGCT GGGGAGCCTG GGGACTTTCC ACACCTGGTT GCTGACTAAT TGAGATGCAT 
     7741 GCTTTGCATA CTTCTGCCTG CTGGGGAGCC TGGGGACTTT CCACACCCTA ACTGACACAC 
     7801 ATTCCACAGC TGGTTCTTTC CGCCTCAGGA CTCTTCCTTT TTCAATATTA TTGAAGCATT 
     7861 TATCAGGGTT ATTGTCTCAT GAGCGGATAC ATATTTGAAT GTATTTAGAA AAATAAACAA 
     7921 ATAGGGGTTC CGCGCACATT TCCCCGAAAA GTGCCACCTG ACGCGCCCTG TAGCGGCGCA 
     7981 TTAAGCGCGG CGGGTGTGGT GGTTACGCGC AGCGTGACCG CTACACTTGC CAGCGCCCTA 
     8041 GCGCCCGCTC CTTTCGCTTT CTTCCCTTCC TTTCTCGCCA CGTTCGCCGG CTTTCCCCGT 
     8101 CAAGCTCTAA ATCGGGGGCT CCCTTTAGGG TTCCGATTTA GTGCTTTACG GCACCTCGAC 
     8161 CCCAAAAAAC TTGATTAGGG TGATGGTTCA CGTAGTGGGC CATCGCCCTG ATAGACGGTT 
     8221 TTTCGCCCTT TGACGTTGGA GTCCACGTTC TTTAATAGTG GACTCTTGTT CCAAACTGGA 
     8281 ACAACACTCA ACCCTATCTC GGTCTATTCT TTTGATTTAT AAGGGATTTT GCCGATTTCG 
     8341 GCCTATTGGT TAAAAAATGA GCTGATTTAA CAAAAATTTA ACGCGAATTT T
//