LOCUS       RC213837                7647 bp    DNA     circular  SYN 
DEFINITION  PGAP1 (Myc-DDK-tagged)-Human post-GPI attachment to proteins 1 (PGAP1)
ACCESSION   NM_024989
VERSION     NM_024989.3
SOURCE      synthetic construct 
FEATURES    Location/Qualifiers 
     misc_feature    201..924 
                     /label="CMV promoter"  
     misc_feature    839..856 
                     /label="Restriction Enzyme"  
     misc_feature    953..971 
                     /label="T7 promoter"  
     misc_feature    3804..3895 
                     /label="Myc-DDK"  
     misc_feature    3925..4510 
                     /label="PolyA signal"  
     misc_feature    3982..4001 
                     /label="Restriction Enzyme"  
     misc_feature    4786..5458 
                     /label="ColE1"  
     misc_feature    5888..6682 
                     /label="Kan/Neo"  
     misc_feature    6717..7019 
                     /label="SV40 ori"  
     misc_feature    7105..7582 
                     /label="f1 ori"  
     misc_feature    1020..1028 
                     /label="Restriction Enzyme"  
     misc_feature    3786..3792 
                     /label="Restriction Enzyme"  
     misc_feature    1020..3786 
                     /label="PGAP1"  
ORIGIN                      
        1 AACAAAATAT TAACGCTTAC AATTTCCATT CGCCATTCAG GCTGCGCAAC TGTTGGGAAG 
       61 GGCGATCGGT GCGGGCCTCT TCGCTATTAC GCCAGCTGGC GAAAGGGGGA TGTGCTGCAA 
      121 GGCGATTAAG TTGGGTAACG CCAGGGTTTT CCCAGTCACG ACGTTGTAAA ACGACGGCCA 
      181 GTGCCAAGCT GATCTATACA TTGAATCAAT ATTGGCAATT AGCCATATTA GTCATTGGTT 
      241 ATATAGCATA AATCAATATT GGCTATTGGC CATTGCATAC GTTGTATCTA TATCATAATA 
      301 TGTACATTTA TATTGGCTCA TGTCCAATAT GACCGCCATG TTGACATTGA TTATTGACTA 
      361 GTTATTAATA GTAATCAATT ACGGGGTCAT TAGTTCATAG CCCATATATG GAGTTCCGCG 
      421 TTACATAACT TACGGTAAAT GGCCCGCCTG GCTGACCGCC CAACGACCCC CGCCCATTGA 
      481 CGTCAATAAT GACGTATGTT CCCATAGTAA CGCCAATAGG GACTTTCCAT TGACGTCAAT 
      541 GGGTGGAGTA TTTACGGTAA ACTGCCCACT TGGCAGTACA TCAAGTGTAT CATATGCCAA 
      601 GTCCGCCCCC TATTGACGTC AATGACGGTA AATGGCCCGC CTGGCATTAT GCCCAGTACA 
      661 TGACCTTACG GGACTTTCCT ACTTGGCAGT ACATCTACGT ATTAGTCATC GCTATTACCA 
      721 TGGTGATGCG GTTTTGGCAG TACACCAATG GGCGTGGATA GCGGTTTGAC TCACGGGGAT 
      781 TTCCAAGTCT CCACCCCATT GACGTCAATG GGAGTTTGTT TTGGCACCAA AATCAACGGG 
      841 ACTTTCCAAA ATGTCGTAAT AACCCCGCCC CGTTGACGCA AATGGGCGGT AGGCGTGTAC 
      901 GGTGGGAGGT CTATATAAGC AGAGCTCGTT TAGTGAACCG TCAGAATTTT GTAATACGAC 
      961 TCACTATAGG GCGGCCGGGA ATTCGTCGAC TGGATCCGGT ACCGAGGAGA TCTGCCGCCG 
     1021 CGATCGCCAT GTTTCTTCAC TCAGTTAATC TCTGGAACCT GGCGTTTTAT GTCTTCATGG 
     1081 TCTTTCTGGC AACCCTGGGG CTGTGGGATG TCTTCTTCGG CTTCGAGGAG AATAAGTGCA 
     1141 GTATGAGCTA CATGTTTGAG TACCCGGAGT ATCAGAAAAT AGAACTTCCA AAGAAACTGG 
     1201 CAAAACGCTA TCCCGCATAT GAGTTGTATC TTTATGGAGA GGGATCCTAT GCTGAAGAAC 
     1261 ACAAAATTCT CCCTTTGACG GGTATTCCAG TTCTCTTTCT TCCTGGTAAT GCTGGAAGTT 
     1321 ATAAGCAAGT TCGTTCTATT GGCTCCATTG CACTTAGAAA AGCAGAGGAC ATTGACTTCA 
     1381 AGTACCACTT TGACTTCTTT AGTGTGAACT TCAATGAAGA ACTGGTGGCT TTGTATGGTG 
     1441 GAAGTCTTCA GAAGCAGACC AAGTTTGTAC ATGAATGTAT TAAAACAATT CTCAAACTCT 
     1501 ATAAGGGTCA AGAATTTGCT CCAAAAAGTG TGGCAATAAT TGGTCATTCT ATGGGTGGCC 
     1561 TTGTTGCAAG AGCATTGCTT ACACTGAAAA ATTTTAAGCA TGATCTGATA AATCTTCTTA 
     1621 TTACACAAGC CACACCTCAT GTTGCTCCTG TGATGCCATT AGATCGTTTC ATTACAGATT 
     1681 TTTATACGAC TGTAAACAAC TATTGGATTC TAAATGCTCG ACACATAAAT TTAACCACAC 
     1741 TTTCTGTAGC TGGAGGATTC CGGGATTACC AAGTTCGTTC AGGATTGACT TTTCTACCAA 
     1801 AATTAAGCCA TCATACCAGT GCCTTATCTG TTGTGAGTTC AGCAGTGCCT AAGACCTGGG 
     1861 TCTCAACAGA CCACCTCTCC ATTGTGTGGT GTAAACAATT GCAGTTGACT ACAGTTCGAG 
     1921 CATTCTTTGA TCTTATTGAT GCTGATACTA AACAAATAAC TCAAAATTCC AAGAAGAAAC 
     1981 TGTCAGTTTT GTATCACCAC TTTATAAGAC ACCCATCAAA ACATTTTGAG GAAAATCCAG 
     2041 CTATAATTTC TGACTTAACA GGGACATCTA TGTGGGTTCT AGTAAAAGTG TCCAAATGGA 
     2101 CCTATGTAGC TTACAACGAA TCTGAGAAGA TATATTTTAC ATTTCCTCTT GAAAATCATA 
     2161 GAAAAATCTA CACTCATGTC TATTGTCAGA GCACTATGCT GGATACAAAT AGTTGGATTT 
     2221 TTGCTTGCAT AAACAGCACT TCTATGTGCC TGCAAGGGGT TGATTTATCA TGGAAAGCTG 
     2281 AACTGCTGCC AACAATTAAG TATCTGACAT TAAGACTTCA AGACTATCCA TCTTTGTCTC 
     2341 ATCTTGTTGT TTATGTACCA TCTGTTCGTG GAAGTAAGTT TGTTGTAGAT TGTGAATTCT 
     2401 TTAAAAAAGA GAAAAGATAC ATACAGCTTC CTGTAACTCA TCTTTTTTCC TTTGGATTGT 
     2461 CTTCAAGGAA AGTGGTGTTA AATACAAATG GCCTATACTA CAATCTAGAG CTTCTGAACT 
     2521 TTGGACAGAT ATACCAAGCT TTTAAAATCA ACGTGGTAAG CAAGTGCTCA GCAGTCAAAG 
     2581 AAGAAATAAC CAGTATCTAT AGACTTCATA TTCCTTGGTC TTATGAAGAT TCACTAACCA 
     2641 TTGCACAGGC TCCATCTTCC ACAGAAATTT CTCTGAAACT CCATATTGCT CAACCAGAAA 
     2701 ACAATACCCA TGTGGCATTA TTTAAAATGT ACACGTCATC AGACTGTCGG TACGAGGTGA 
     2761 CAGTTAAGAC TTCCTTTTCA CAAATACTTG GACAGGTAGT TAGATTTCAT GGTGGAGCTC 
     2821 TTCCTGCTTA TGTCGTATCT AATATCCTTC TTGCTTATAG AGGACAGTTA TATTCTCTTT 
     2881 TCTCAACAGG TTGTTGCTTA GAATATGCTA CCATGTTGGA TAAAGAAGCC AAACCATACA 
     2941 AAGTTGATCC TTTTGTAATT ATCATTAAGT TTCTGTTGGG GTATAAATGG TTTAAAGAAT 
     3001 TGTGGGATGT ATTATTGTTA CCAGAATTAG ATGCCGTCAT TTTAACTTGT CAGAGTATGT 
     3061 GTTTCCCCCT GATATCCTTG ATTCTCTTTC TGTTTGGAAC GTGTACTGCC TACTGGAGTG 
     3121 GCCTACTGTC TTCTGCATCT GTGAGACTTC TTTCTTCATT GTGGCTAGCT TTGAAAAGAC 
     3181 CCTCTGAACT TCCTAAAGAT ATCAAGATGA TATCACCAGA CTTGCCCTTT TTGACAATTG 
     3241 TCTTGATCAT AGTTAGTTGG ACAACTTGTG GAGCACTAGC CATACTTCTT TCTTATCTTT 
     3301 ACTATGTGTT TAAGGTTGTT CATCTGCAAG CCAGCTTAAC AACTTTTAAG AATAGCCAGC 
     3361 CTGTGAATCC CAAACACTCT AGAAGAAGTG AAAAGAAATC CAATCATCAT AAAGACTCCT 
     3421 CAATACACCA TCTTCGTTTA TCTGCCAACG ATGCTGAAGA TAGCCTTCGC ATGCACAGTA 
     3481 CTGTGATTAA CTTACTAACA TGGATTGTAT TACTCAGCAT GCCTTCTCTA ATTTATTGGC 
     3541 TAAAGAATCT TAGGTATTAT TTTAAACTTA ATCCTGATCC ATGTAAACCT TTGGCATTTA 
     3601 TCCTTATTCC GACTATGGCA ATTCTTGGAA ATACTTACAC TGTTTCAATA AAATCAAGTA 
     3661 AATTGTTGAA GACTACTTCA CAATTTCCAC TTCCTCTGGC TGTTGGTGTG ATTGCTTTTG 
     3721 GGTCAGCACA TTTATATAGG CTTCCATGCT TTGTCTTCAT TCCTCTTTTA CTCCATGCAT 
     3781 TATGCAACTT TATGACGCGT ACGCGGCCGC TCGAGCAGAA ACTCATCTCA GAAGAGGATC 
     3841 TGGCAGCAAA TGATATCCTG GATTACAAGG ATGACGACGA TAAGGTTTAA ACGGCCGGCC 
     3901 GCGGTCATAG CTGTTTCCTG AACAGATCCC GGGTGGCATC CCTGTGACCC CTCCCCAGTG 
     3961 CCTCTCCTGG CCCTGGAAGT TGCCACTCCA GTGCCCACCA GCCTTGTCCT AATAAAATTA 
     4021 AGTTGCATCA TTTTGTCTGA CTAGGTGTCC TTCTATAATA TTATGGGGTG GAGGGGGGTG 
     4081 GTATGGAGCA AGGGGCAAGT TGGGAAGACA ACCTGTAGGG CCTGCGGGGT CTATTGGGAA 
     4141 CCAAGCTGGA GTGCAGTGGC ACAATCTTGG CTCACTGCAA TCTCCGCCTC CTGGGTTCAA 
     4201 GCGATTCTCC TGCCTCAGCC TCCCGAGTTG TTGGGATTCC AGGCATGCAT GACCAGGCTC 
     4261 AGCTAATTTT TGTTTTTTTG GTAGAGACGG GGTTTCACCA TATTGGCCAG GCTGGTCTCC 
     4321 AACTCCTAAT CTCAGGTGAT CTACCCACCT TGGCCTCCCA AATTGCTGGG ATTACAGGCG 
     4381 TGAACCACTG CTCCCTTCCC TGTCCTTCTG ATTTTAAAAT AACTATACCA GCAGGAGGAC 
     4441 GTCCAGACAC AGCATAGGCT ACCTGGCCAT GCCCAACCGG TGGGACATTT GAGTTGCTTG 
     4501 CTTGGCACTG TCCTCTCATG CGTTGGGTCC ACTCAGTAGA TGCCTGTTGA ATTGGGTACG 
     4561 CGGCCAGCGG CGAGCGGTAT CAGCTCACTC AAAGGCGGTA ATACGGTTAT CCACAGAATC 
     4621 AGGGGATAAC GCAGGAAAGA ACATGTGAGC AAAAGGCCAG CAAAAGGCCA GGAACCGTAA 
     4681 AAAGGCCGCG TTGCTGGCGT TTTTCCATAG GCTCCGCCCC CCTGACGAGC ATCACAAAAA 
     4741 TCGACGCTCA AGTCAGAGGT GGCGAAACCC GACAGGACTA TAAAGATACC AGGCGTTTCC 
     4801 CCCTGGAAGC TCCCTCGTGC GCTCTCCTGT TCCGACCCTG CCGCTTACCG GATACCTGTC 
     4861 CGCCTTTCTC CCTTCGGGAA GCGTGGCGCT TTCTCATAGC TCACGCTGTA GGTATCTCAG 
     4921 TTCGGTGTAG GTCGTTCGCT CCAAGCTGGG CTGTGTGCAC GAACCCCCCG TTCAGCCCGA 
     4981 CCGCTGCGCC TTATCCGGTA ACTATCGTCT TGAGTCCAAC CCGGTAAGAC ACGACTTATC 
     5041 GCCACTGGCA GCAGCCACTG GTAACAGGAT TAGCAGAGCG AGGTATGTAG GCGGTGCTAC 
     5101 AGAGTTCTTG AAGTGGTGGC CTAACTACGG CTACACTAGA AGAACAGTAT TTGGTATCTG 
     5161 CGCTCTGCTG AAGCCAGTTA CCTTCGGAAA AAGAGTTGGT AGCTCTTGAT CCGGCAAACA 
     5221 AACCACCGCT GGTAGCGGTG GTTTTTTTGT TTGCAAGCAG CAGATTACGC GCAGAAAAAA 
     5281 AGGATCTCAA GAAGATCCTT TGATCTTTTC TACGGGGTCT GACGCTCAGT GGAACGAAAA 
     5341 CTCACGTTAA GGGATTTTGG TCATGAGATT ATCAAAAAGG ATCTTCACCT AGATCCTTTT 
     5401 AAATTAAAAA TGAAGTTTTA AATCAATCTA AAGTATATAT GAGTAACCTG AGGCTATGGC 
     5461 AGGGCCTGCC GCCCCGACGT TGGCTGCGAG CCCTGGGCCT TCACCCGAAC TTGGGGGGTG 
     5521 GGGTGGGGAA AAGGAAGAAA CGCGGGCGTA TTGGCCCCAA TGGGGTCTCG GTGGGGTATC 
     5581 GACAGAGTGC CAGCCCTGGG ACCGAACCCC GCGTTTATGA ACAAACGACC CAACACCGTG 
     5641 CGTTTTATTC TGTCTTTTTA TTGCCGTCAT AGCGCGGGTT CCTTCCGGTA TTGTCTCCTT 
     5701 CCGTGTTTCA GTTAGCCTCC CCCTAGGGTG GGCGAAGAAC TCCAGCATGA GATCCCCGCG 
     5761 CTGGAGGATC ATCCAGCCGG CGTCCCGGAA AACGATTCCG AAGCCCAACC TTTCATAGAA 
     5821 GGCGGCGGTG GAATCGAAAT CTCGTGATGG CAGGTTGGGC GTCGCTTGGT CGGTCATTTC 
     5881 GAACCCCAGA GTCCCGCTCA GAAGAACTCG TCAAGAAGGC GATAGAAGGC GATGCGCTGC 
     5941 GAATCGGGAG CGGCGATACC GTAAAGCACG AGGAAGCGGT CAGCCCATTC GCCGCCAAGC 
     6001 TCTTCAGCAA TATCACGGGT AGCCAACGCT ATGTCCTGAT AGCGATCCGC CACACCCAGC 
     6061 CGGCCACAGT CGATGAATCC AGAAAAGCGG CCATTTTCCA CCATGATATT CGGCAAGCAG 
     6121 GCATCGCCAT GGGTCACGAC GAGATCCTCG CCGTCGGGCA TGCTCGCCTT GAGCCTGGCG 
     6181 AACAGTTCGG CTGGCGCGAG CCCCTGATGC TCTTCGTCCA GATCATCCTG ATCGACAAGA 
     6241 CCGGCTTCCA TCCGAGTACG TGCTCGCTCG ATGCGATGTT TCGCTTGGTG GTCGAATGGG 
     6301 CAGGTAGCCG GATCAAGCGT ATGCAGCCGC CGCATTGCAT CAGCCATGAT GGATACTTTC 
     6361 TCGGCAGGAG CAAGGTGAGA TGACAGGAGA TCCTGCCCCG GCACTTCGCC CAATAGCAGC 
     6421 CAGTCCCTTC CCGCTTCAGT GACAACGTCG AGCACAGCTG CGCAAGGAAC GCCCGTCGTG 
     6481 GCCAGCCACG ATAGCCGCGC TGCCTCGTCT TGCAGTTCAT TCAGGGCACC GGACAGGTCG 
     6541 GTCTTGACAA AAAGAACCGG GCGCCCCTGC GCTGACAGCC GGAACACGGC GGCATCAGAG 
     6601 CAGCCGATTG TCTGTTGTGC CCAGTCATAG CCGAATAGCC TCTCCACCCA AGCGGCCGGA 
     6661 GAACCTGCGT GCAATCCATC TTGTTCAATC ATGCGAAACG ATCCTCATCC TGTCTCTTGA 
     6721 TCGATCTTTG CAAAAGCCTA GGCCTCCAAA AAAGCCTCCT CACTACTTCT GGAATAGCTC 
     6781 AGAGGCCGAG GCGGCCTCGG CCTCTGCATA AATAAAAAAA ATTAGTCAGC CATGGGGCGG 
     6841 AGAATGGGCG GAACTGGGCG GAGTTAGGGG CGGGATGGGC GGAGTTAGGG GCGGGACTAT 
     6901 GGTTGCTGAC TAATTGAGAT GCATGCTTTG CATACTTCTG CCTGCTGGGG AGCCTGGGGA 
     6961 CTTTCCACAC CTGGTTGCTG ACTAATTGAG ATGCATGCTT TGCATACTTC TGCCTGCTGG 
     7021 GGAGCCTGGG GACTTTCCAC ACCCTAACTG ACACACATTC CACAGCTGGT TCTTTCCGCC 
     7081 TCAGGACTCT TCCTTTTTCA ATATTATTGA AGCATTTATC AGGGTTATTG TCTCATGAGC 
     7141 GGATACATAT TTGAATGTAT TTAGAAAAAT AAACAAATAG GGGTTCCGCG CACATTTCCC 
     7201 CGAAAAGTGC CACCTGACGC GCCCTGTAGC GGCGCATTAA GCGCGGCGGG TGTGGTGGTT 
     7261 ACGCGCAGCG TGACCGCTAC ACTTGCCAGC GCCCTAGCGC CCGCTCCTTT CGCTTTCTTC 
     7321 CCTTCCTTTC TCGCCACGTT CGCCGGCTTT CCCCGTCAAG CTCTAAATCG GGGGCTCCCT 
     7381 TTAGGGTTCC GATTTAGTGC TTTACGGCAC CTCGACCCCA AAAAACTTGA TTAGGGTGAT 
     7441 GGTTCACGTA GTGGGCCATC GCCCTGATAG ACGGTTTTTC GCCCTTTGAC GTTGGAGTCC 
     7501 ACGTTCTTTA ATAGTGGACT CTTGTTCCAA ACTGGAACAA CACTCAACCC TATCTCGGTC 
     7561 TATTCTTTTG ATTTATAAGG GATTTTGCCG ATTTCGGCCT ATTGGTTAAA AAATGAGCTG 
     7621 ATTTAACAAA AATTTAACGC GAATTTT
//