LOCUS       RC213835                8496 bp    DNA     circular  SYN 
DEFINITION  ATP2B4 (Myc-DDK-tagged)-Human ATPase, Ca++ transporting, plasma membrane 4 (ATP2B4), transcript variant 2
ACCESSION   NM_001684
VERSION     NM_001684.3
SOURCE      synthetic construct 
FEATURES    Location/Qualifiers 
     misc_feature    201..924 
                     /label="CMV promoter"  
     misc_feature    839..856 
                     /label="Restriction Enzyme"  
     misc_feature    953..971 
                     /label="T7 promoter"  
     misc_feature    4653..4744 
                     /label="Myc-DDK"  
     misc_feature    4774..5359 
                     /label="PolyA signal"  
     misc_feature    4831..4850 
                     /label="Restriction Enzyme"  
     misc_feature    5635..6307 
                     /label="ColE1"  
     misc_feature    6737..7531 
                     /label="Kan/Neo"  
     misc_feature    7566..7868 
                     /label="SV40 ori"  
     misc_feature    7954..8431 
                     /label="f1 ori"  
     misc_feature    1020..1028 
                     /label="Restriction Enzyme"  
     misc_feature    4635..4641 
                     /label="Restriction Enzyme"  
     misc_feature    1020..4635 
                     /label="ATP2B4"  
ORIGIN                      
        1 AACAAAATAT TAACGCTTAC AATTTCCATT CGCCATTCAG GCTGCGCAAC TGTTGGGAAG 
       61 GGCGATCGGT GCGGGCCTCT TCGCTATTAC GCCAGCTGGC GAAAGGGGGA TGTGCTGCAA 
      121 GGCGATTAAG TTGGGTAACG CCAGGGTTTT CCCAGTCACG ACGTTGTAAA ACGACGGCCA 
      181 GTGCCAAGCT GATCTATACA TTGAATCAAT ATTGGCAATT AGCCATATTA GTCATTGGTT 
      241 ATATAGCATA AATCAATATT GGCTATTGGC CATTGCATAC GTTGTATCTA TATCATAATA 
      301 TGTACATTTA TATTGGCTCA TGTCCAATAT GACCGCCATG TTGACATTGA TTATTGACTA 
      361 GTTATTAATA GTAATCAATT ACGGGGTCAT TAGTTCATAG CCCATATATG GAGTTCCGCG 
      421 TTACATAACT TACGGTAAAT GGCCCGCCTG GCTGACCGCC CAACGACCCC CGCCCATTGA 
      481 CGTCAATAAT GACGTATGTT CCCATAGTAA CGCCAATAGG GACTTTCCAT TGACGTCAAT 
      541 GGGTGGAGTA TTTACGGTAA ACTGCCCACT TGGCAGTACA TCAAGTGTAT CATATGCCAA 
      601 GTCCGCCCCC TATTGACGTC AATGACGGTA AATGGCCCGC CTGGCATTAT GCCCAGTACA 
      661 TGACCTTACG GGACTTTCCT ACTTGGCAGT ACATCTACGT ATTAGTCATC GCTATTACCA 
      721 TGGTGATGCG GTTTTGGCAG TACACCAATG GGCGTGGATA GCGGTTTGAC TCACGGGGAT 
      781 TTCCAAGTCT CCACCCCATT GACGTCAATG GGAGTTTGTT TTGGCACCAA AATCAACGGG 
      841 ACTTTCCAAA ATGTCGTAAT AACCCCGCCC CGTTGACGCA AATGGGCGGT AGGCGTGTAC 
      901 GGTGGGAGGT CTATATAAGC AGAGCTCGTT TAGTGAACCG TCAGAATTTT GTAATACGAC 
      961 TCACTATAGG GCGGCCGGGA ATTCGTCGAC TGGATCCGGT ACCGAGGAGA TCTGCCGCCG 
     1021 CGATCGCCAT GACGAACCCA TCAGACCGTG TCTTGCCTGC CAACTCGATG GCCGAGAGCC 
     1081 GTGAAGGGGA CTTTGGCTGC ACAGTAATGG AACTGAGGAA GCTCATGGAG CTGCGTTCGA 
     1141 GGGATGCACT GACCCAGATT AATGTCCACT ATGGAGGTGT ACAGAATCTC TGCAGTAGAC 
     1201 TGAAAACCTC CCCTGTGGAA GGTCTGTCTG GGAACCCTGC AGATCTGGAG AAACGTAGGC 
     1261 AGGTGTTTGG ACACAACGTG ATCCCCCCCA AAAAGCCCAA GACTTTCTTA GAATTAGTGT 
     1321 GGGAAGCTCT TCAAGATGTC ACGCTCATCA TCCTGGAGAT TGCAGCCATC ATCTCCCTGG 
     1381 TCCTGTCCTT TTATCGCCCT GCTGGTGAAG AAAATGAACT GTGTGGTCAA GTCGCAACTA 
     1441 CCCCAGAAGA TGAAAATGAG GCACAAGCTG GCTGGATTGA GGGGGCAGCC ATCCTTTTCT 
     1501 CAGTGATCAT CGTGGTGTTA GTGACTGCCT TTAATGATTG GAGCAAAGAG AAGCAATTCC 
     1561 GGGGGCTGCA GTGCCGCATT GAACAGGAGC AAAAGTTCTC CATCATCCGA AACGGTCAAC 
     1621 TCATCCAGCT CCCTGTGGCT GAGATTGTGG TTGGTGATAT TGCCCAAGTC AAATACGGTG 
     1681 ATCTGCTGCC TGCAGATGGA ATCCTGATCC AAGGAAATGA TCTGAAGATT GATGAGAGCT 
     1741 CTCTGACAGG GGAATCTGAC CATGTCAAGA AGTCCCTGGA CAAAGACCCC ATGTTGCTCT 
     1801 CAGGGACCCA TGTCATGGAA GGTTCTGGCC GGATGGTGGT GACAGCTGTT GGTGTCAACT 
     1861 CTCAGACTGG AATCATCCTT ACTCTCTTGG GGGTCAATGA GGATGACGAA GGGGAGAAAA 
     1921 AGAAGAAAGG TAAAAAACAA GGAGTCCCTG AAAATCGCAA CAAAGCAAAG ACCCAAGACG 
     1981 GAGTGGCCCT GGAAATCCAG CCACTCAACA GCCAGGAGGG AATCGACAAT GAGGAAAAGG 
     2041 ACAAGAAGGC AGTCAAGGTG CCTAAAAAGG AGAAGTCAGT GCTGCAGGGC AAGCTGACTC 
     2101 GCCTGGCTGT TCAGATTGGG AAAGCCGGTC TGCTCATGTC TGCTCTCACG GTTTTCATCC 
     2161 TGATTCTATA CTTTGTGATT GACAACTTTG TGATAAATCG CAGACCATGG CTCCCTGAGT 
     2221 GTACTCCCAT CTACATCCAG TACTTTGTCA AGTTCTTCAT CATCGGCATC ACTGTACTGG 
     2281 TGGTGGCTGT GCCAGAGGGG CTGCCTCTGG CTGTCACCAT CTCACTGGCC TACTCTGTGA 
     2341 AGAAAATGAT GAAAGACAAT AACCTAGTAC GGCACTTGGA TGCTTGTGAG ACCATGGGCA 
     2401 ACGCCACCGC CATCTGCTCT GATAAGACAG GCACGTTGAC CATGAACCGC ATGACTGTGG 
     2461 TACAAGCTTA TATTGGGGGC ATCCATTACC GTCAAATCCC AAGCCCTGAT GTCTTCCTGC 
     2521 CCAAAGTCCT GGACCTCATT GTCAATGGCA TTTCTATCAA CAGTGCTTAT ACCTCCAAGA 
     2581 TTCTGCCTCC AGAGAAGGAG GGAGGCCTGC CTCGGCAGGT GGGCAACAAG ACCGAGTGTG 
     2641 CTCTGCTAGG CTTTGTCACA GATCTGAAGC AGGATTATCA GGCTGTGCGT AATGAAGTGC 
     2701 CCGAGGAGAA GCTCTACAAG GTGTACACCT TTAACTCAGT GCGCAAGTCA ATGAGCACCG 
     2761 TCATCAGGAA TCCCAACGGT GGCTTCCGTA TGTACAGCAA GGGCGCCTCT GAGATCATCT 
     2821 TGCGCAAGTG TAATCGAATC CTGGACCGGA AAGGGGAAGC AGTGCCATTC AAGAATAAAG 
     2881 ACAGAGATGA TATGGTACGC ACTGTCATCG AGCCCATGGC CTGTGATGGA CTCCGGACTA 
     2941 TCTGCATAGC TTACCGGGAC TTCGATGACA CAGAGCCCTC TTGGGACAAT GAGAATGAGA 
     3001 TCCTCACCGA ACTGACCTGT ATCGCGGTGG TGGGCATTGA GGACCCTGTG CGCCCAGAGG 
     3061 TGCCAGATGC TATTGCCAAA TGCAAACAAG CTGGCATTAC TGTCAGAATG GTGACAGGTG 
     3121 ACAACATCAA CACAGCCCGG GCCATTGCCA CCAAATGTGG CATTCTGACA CCTGGGGATG 
     3181 ACTTCCTGTG CTTAGAAGGC AAAGAATTCA ACCGGCTCAT CCGCAACGAG AAAGGCGAGG 
     3241 TAGAGCAAGA AAAGCTGGAC AAGATCTGGC CTAAGCTTCG GGTCCTGGCG CGATCTTCTC 
     3301 CCACTGACAA GCACACCCTG GTGAAAGGCA TAATTGACAG CACTGTTGGG GAACACCGGC 
     3361 AGGTCGTGGC TGTCACTGGT GATGGCACAA ATGACGGGCC TGCTCTGAAG AAAGCGGATG 
     3421 TTGGTTTTGC CATGGGCATC GCAGGCACAG ATGTAGCAAA GGAGGCTTCA GACATCATCC 
     3481 TAACAGATGA CAACTTCACC AGCATTGTGA AGGCAGTGAT GTGGGGACGA AATGTCTATG 
     3541 ACAGCATCTC CAAGTTCCTG CAGTTCCAGC TCACTGTCAA TGTGGTGGCC GTGATTGTAG 
     3601 CCTTCACTGG AGCCTGTATC ACTCAGGATT CCCCATTGAA AGCTGTGCAG ATGTTGTGGG 
     3661 TTAATCTGAT CATGGACACT TTTGCTTCAT TGGCCCTGGC CACAGAGCCC CCTACGGAAT 
     3721 CTCTGTTGAA GCGGCGCCCC TATGGCCGAA ATAAGCCTCT GATCTCACGC ACTATGATGA 
     3781 AGAACATCTT GGGCCATGCA TTCTATCAGC TCATTGTCAT CTTTATCCTT GTCTTTGCGG 
     3841 GTGAGAAATT CTTTGATATT GATAGTGGGA GGAAGGCACC TCTACATTCA CCACCCAGCC 
     3901 AGCACTATAC CATTGTTTTT AACACCTTCG TGCTGATGCA GCTCTTCAAT GAAATCAACT 
     3961 CCCGAAAGAT CCATGGAGAG AAGAACGTCT TTTCAGGCAT CTACCGCAAC ATTATCTTCT 
     4021 GCTCTGTAGT CTTGGGCACA TTCATCTGCC AGATTTTCAT CGTGGAATTT GGGGGTAAAC 
     4081 CCTTCAGTTG TACAAGCCTC AGCCTGTCTC AGTGGCTGTG GTGTCTCTTC ATTGGGATTG 
     4141 GAGAACTTCT GTGGGGCCAG TTCATCTCCG CAATACCTAC CCGATCCCTG AAGTTCCTGA 
     4201 AGGAGGCTGG GCATGGCACC ACCAAAGAGG AGATCACCAA GGATGCCGAG GGACTGGATG 
     4261 AGATTGACCA TGCTGAGATG GAGCTGCGCC GAGGCCAGAT CCTCTGGTTC CGGGGCCTGA 
     4321 ACCGTATCCA GACTCAGATC AAAGTGGTCA AAGCGTTCCA TAGTTCCCTC CACGAAAGCA 
     4381 TTCAGAAACC CTACAACCAA AAGTCCATCC ACAGCTTCAT GACCCACCCT GAATTCGCCA 
     4441 TAGAGGAGGA GTTGCCACGA ACACCACTCC TGGATGAGGA AGAGGAGGAA AATCCTGACA 
     4501 AGGCTTCTAA GTTTGGGACT AGGGTGCTCC TGTTGGATGG TGAGGTCACT CCATATGCCA 
     4561 ATACAAACAA CAATGCGGTG GATTGCAACC AAGTGCAGCT CCCCCAGTCG GACAGCTCTC 
     4621 TACAGAGCCT AGAGACATCA GTTACGCGTA CGCGGCCGCT CGAGCAGAAA CTCATCTCAG 
     4681 AAGAGGATCT GGCAGCAAAT GATATCCTGG ATTACAAGGA TGACGACGAT AAGGTTTAAA 
     4741 CGGCCGGCCG CGGTCATAGC TGTTTCCTGA ACAGATCCCG GGTGGCATCC CTGTGACCCC 
     4801 TCCCCAGTGC CTCTCCTGGC CCTGGAAGTT GCCACTCCAG TGCCCACCAG CCTTGTCCTA 
     4861 ATAAAATTAA GTTGCATCAT TTTGTCTGAC TAGGTGTCCT TCTATAATAT TATGGGGTGG 
     4921 AGGGGGGTGG TATGGAGCAA GGGGCAAGTT GGGAAGACAA CCTGTAGGGC CTGCGGGGTC 
     4981 TATTGGGAAC CAAGCTGGAG TGCAGTGGCA CAATCTTGGC TCACTGCAAT CTCCGCCTCC 
     5041 TGGGTTCAAG CGATTCTCCT GCCTCAGCCT CCCGAGTTGT TGGGATTCCA GGCATGCATG 
     5101 ACCAGGCTCA GCTAATTTTT GTTTTTTTGG TAGAGACGGG GTTTCACCAT ATTGGCCAGG 
     5161 CTGGTCTCCA ACTCCTAATC TCAGGTGATC TACCCACCTT GGCCTCCCAA ATTGCTGGGA 
     5221 TTACAGGCGT GAACCACTGC TCCCTTCCCT GTCCTTCTGA TTTTAAAATA ACTATACCAG 
     5281 CAGGAGGACG TCCAGACACA GCATAGGCTA CCTGGCCATG CCCAACCGGT GGGACATTTG 
     5341 AGTTGCTTGC TTGGCACTGT CCTCTCATGC GTTGGGTCCA CTCAGTAGAT GCCTGTTGAA 
     5401 TTGGGTACGC GGCCAGCGGC GAGCGGTATC AGCTCACTCA AAGGCGGTAA TACGGTTATC 
     5461 CACAGAATCA GGGGATAACG CAGGAAAGAA CATGTGAGCA AAAGGCCAGC AAAAGGCCAG 
     5521 GAACCGTAAA AAGGCCGCGT TGCTGGCGTT TTTCCATAGG CTCCGCCCCC CTGACGAGCA 
     5581 TCACAAAAAT CGACGCTCAA GTCAGAGGTG GCGAAACCCG ACAGGACTAT AAAGATACCA 
     5641 GGCGTTTCCC CCTGGAAGCT CCCTCGTGCG CTCTCCTGTT CCGACCCTGC CGCTTACCGG 
     5701 ATACCTGTCC GCCTTTCTCC CTTCGGGAAG CGTGGCGCTT TCTCATAGCT CACGCTGTAG 
     5761 GTATCTCAGT TCGGTGTAGG TCGTTCGCTC CAAGCTGGGC TGTGTGCACG AACCCCCCGT 
     5821 TCAGCCCGAC CGCTGCGCCT TATCCGGTAA CTATCGTCTT GAGTCCAACC CGGTAAGACA 
     5881 CGACTTATCG CCACTGGCAG CAGCCACTGG TAACAGGATT AGCAGAGCGA GGTATGTAGG 
     5941 CGGTGCTACA GAGTTCTTGA AGTGGTGGCC TAACTACGGC TACACTAGAA GAACAGTATT 
     6001 TGGTATCTGC GCTCTGCTGA AGCCAGTTAC CTTCGGAAAA AGAGTTGGTA GCTCTTGATC 
     6061 CGGCAAACAA ACCACCGCTG GTAGCGGTGG TTTTTTTGTT TGCAAGCAGC AGATTACGCG 
     6121 CAGAAAAAAA GGATCTCAAG AAGATCCTTT GATCTTTTCT ACGGGGTCTG ACGCTCAGTG 
     6181 GAACGAAAAC TCACGTTAAG GGATTTTGGT CATGAGATTA TCAAAAAGGA TCTTCACCTA 
     6241 GATCCTTTTA AATTAAAAAT GAAGTTTTAA ATCAATCTAA AGTATATATG AGTAACCTGA 
     6301 GGCTATGGCA GGGCCTGCCG CCCCGACGTT GGCTGCGAGC CCTGGGCCTT CACCCGAACT 
     6361 TGGGGGGTGG GGTGGGGAAA AGGAAGAAAC GCGGGCGTAT TGGCCCCAAT GGGGTCTCGG 
     6421 TGGGGTATCG ACAGAGTGCC AGCCCTGGGA CCGAACCCCG CGTTTATGAA CAAACGACCC 
     6481 AACACCGTGC GTTTTATTCT GTCTTTTTAT TGCCGTCATA GCGCGGGTTC CTTCCGGTAT 
     6541 TGTCTCCTTC CGTGTTTCAG TTAGCCTCCC CCTAGGGTGG GCGAAGAACT CCAGCATGAG 
     6601 ATCCCCGCGC TGGAGGATCA TCCAGCCGGC GTCCCGGAAA ACGATTCCGA AGCCCAACCT 
     6661 TTCATAGAAG GCGGCGGTGG AATCGAAATC TCGTGATGGC AGGTTGGGCG TCGCTTGGTC 
     6721 GGTCATTTCG AACCCCAGAG TCCCGCTCAG AAGAACTCGT CAAGAAGGCG ATAGAAGGCG 
     6781 ATGCGCTGCG AATCGGGAGC GGCGATACCG TAAAGCACGA GGAAGCGGTC AGCCCATTCG 
     6841 CCGCCAAGCT CTTCAGCAAT ATCACGGGTA GCCAACGCTA TGTCCTGATA GCGATCCGCC 
     6901 ACACCCAGCC GGCCACAGTC GATGAATCCA GAAAAGCGGC CATTTTCCAC CATGATATTC 
     6961 GGCAAGCAGG CATCGCCATG GGTCACGACG AGATCCTCGC CGTCGGGCAT GCTCGCCTTG 
     7021 AGCCTGGCGA ACAGTTCGGC TGGCGCGAGC CCCTGATGCT CTTCGTCCAG ATCATCCTGA 
     7081 TCGACAAGAC CGGCTTCCAT CCGAGTACGT GCTCGCTCGA TGCGATGTTT CGCTTGGTGG 
     7141 TCGAATGGGC AGGTAGCCGG ATCAAGCGTA TGCAGCCGCC GCATTGCATC AGCCATGATG 
     7201 GATACTTTCT CGGCAGGAGC AAGGTGAGAT GACAGGAGAT CCTGCCCCGG CACTTCGCCC 
     7261 AATAGCAGCC AGTCCCTTCC CGCTTCAGTG ACAACGTCGA GCACAGCTGC GCAAGGAACG 
     7321 CCCGTCGTGG CCAGCCACGA TAGCCGCGCT GCCTCGTCTT GCAGTTCATT CAGGGCACCG 
     7381 GACAGGTCGG TCTTGACAAA AAGAACCGGG CGCCCCTGCG CTGACAGCCG GAACACGGCG 
     7441 GCATCAGAGC AGCCGATTGT CTGTTGTGCC CAGTCATAGC CGAATAGCCT CTCCACCCAA 
     7501 GCGGCCGGAG AACCTGCGTG CAATCCATCT TGTTCAATCA TGCGAAACGA TCCTCATCCT 
     7561 GTCTCTTGAT CGATCTTTGC AAAAGCCTAG GCCTCCAAAA AAGCCTCCTC ACTACTTCTG 
     7621 GAATAGCTCA GAGGCCGAGG CGGCCTCGGC CTCTGCATAA ATAAAAAAAA TTAGTCAGCC 
     7681 ATGGGGCGGA GAATGGGCGG AACTGGGCGG AGTTAGGGGC GGGATGGGCG GAGTTAGGGG 
     7741 CGGGACTATG GTTGCTGACT AATTGAGATG CATGCTTTGC ATACTTCTGC CTGCTGGGGA 
     7801 GCCTGGGGAC TTTCCACACC TGGTTGCTGA CTAATTGAGA TGCATGCTTT GCATACTTCT 
     7861 GCCTGCTGGG GAGCCTGGGG ACTTTCCACA CCCTAACTGA CACACATTCC ACAGCTGGTT 
     7921 CTTTCCGCCT CAGGACTCTT CCTTTTTCAA TATTATTGAA GCATTTATCA GGGTTATTGT 
     7981 CTCATGAGCG GATACATATT TGAATGTATT TAGAAAAATA AACAAATAGG GGTTCCGCGC 
     8041 ACATTTCCCC GAAAAGTGCC ACCTGACGCG CCCTGTAGCG GCGCATTAAG CGCGGCGGGT 
     8101 GTGGTGGTTA CGCGCAGCGT GACCGCTACA CTTGCCAGCG CCCTAGCGCC CGCTCCTTTC 
     8161 GCTTTCTTCC CTTCCTTTCT CGCCACGTTC GCCGGCTTTC CCCGTCAAGC TCTAAATCGG 
     8221 GGGCTCCCTT TAGGGTTCCG ATTTAGTGCT TTACGGCACC TCGACCCCAA AAAACTTGAT 
     8281 TAGGGTGATG GTTCACGTAG TGGGCCATCG CCCTGATAGA CGGTTTTTCG CCCTTTGACG 
     8341 TTGGAGTCCA CGTTCTTTAA TAGTGGACTC TTGTTCCAAA CTGGAACAAC ACTCAACCCT 
     8401 ATCTCGGTCT ATTCTTTTGA TTTATAAGGG ATTTTGCCGA TTTCGGCCTA TTGGTTAAAA 
     8461 AATGAGCTGA TTTAACAAAA ATTTAACGCG AATTTT
//