LOCUS       RC213772                7797 bp    DNA     circular  SYN 
DEFINITION  KIT (Myc-DDK-tagged)-Human v-kit Hardy-Zuckerman 4 feline sarcoma viral oncogene homolog (KIT), transcript variant 2
ACCESSION   NM_001093772
VERSION     NM_001093772.1
SOURCE      synthetic construct 
FEATURES    Location/Qualifiers 
     misc_feature    201..924 
                     /label="CMV promoter"  
     misc_feature    839..856 
                     /label="Restriction Enzyme"  
     misc_feature    953..971 
                     /label="T7 promoter"  
     misc_feature    3954..4045 
                     /label="Myc-DDK"  
     misc_feature    4075..4660 
                     /label="PolyA signal"  
     misc_feature    4132..4151 
                     /label="Restriction Enzyme"  
     misc_feature    4936..5608 
                     /label="ColE1"  
     misc_feature    6038..6832 
                     /label="Kan/Neo"  
     misc_feature    6867..7169 
                     /label="SV40 ori"  
     misc_feature    7255..7732 
                     /label="f1 ori"  
     misc_feature    1020..1028 
                     /label="Restriction Enzyme"  
     misc_feature    3936..3942 
                     /label="Restriction Enzyme"  
     misc_feature    1020..3936 
                     /label="KIT"  
ORIGIN                      
        1 AACAAAATAT TAACGCTTAC AATTTCCATT CGCCATTCAG GCTGCGCAAC TGTTGGGAAG 
       61 GGCGATCGGT GCGGGCCTCT TCGCTATTAC GCCAGCTGGC GAAAGGGGGA TGTGCTGCAA 
      121 GGCGATTAAG TTGGGTAACG CCAGGGTTTT CCCAGTCACG ACGTTGTAAA ACGACGGCCA 
      181 GTGCCAAGCT GATCTATACA TTGAATCAAT ATTGGCAATT AGCCATATTA GTCATTGGTT 
      241 ATATAGCATA AATCAATATT GGCTATTGGC CATTGCATAC GTTGTATCTA TATCATAATA 
      301 TGTACATTTA TATTGGCTCA TGTCCAATAT GACCGCCATG TTGACATTGA TTATTGACTA 
      361 GTTATTAATA GTAATCAATT ACGGGGTCAT TAGTTCATAG CCCATATATG GAGTTCCGCG 
      421 TTACATAACT TACGGTAAAT GGCCCGCCTG GCTGACCGCC CAACGACCCC CGCCCATTGA 
      481 CGTCAATAAT GACGTATGTT CCCATAGTAA CGCCAATAGG GACTTTCCAT TGACGTCAAT 
      541 GGGTGGAGTA TTTACGGTAA ACTGCCCACT TGGCAGTACA TCAAGTGTAT CATATGCCAA 
      601 GTCCGCCCCC TATTGACGTC AATGACGGTA AATGGCCCGC CTGGCATTAT GCCCAGTACA 
      661 TGACCTTACG GGACTTTCCT ACTTGGCAGT ACATCTACGT ATTAGTCATC GCTATTACCA 
      721 TGGTGATGCG GTTTTGGCAG TACACCAATG GGCGTGGATA GCGGTTTGAC TCACGGGGAT 
      781 TTCCAAGTCT CCACCCCATT GACGTCAATG GGAGTTTGTT TTGGCACCAA AATCAACGGG 
      841 ACTTTCCAAA ATGTCGTAAT AACCCCGCCC CGTTGACGCA AATGGGCGGT AGGCGTGTAC 
      901 GGTGGGAGGT CTATATAAGC AGAGCTCGTT TAGTGAACCG TCAGAATTTT GTAATACGAC 
      961 TCACTATAGG GCGGCCGGGA ATTCGTCGAC TGGATCCGGT ACCGAGGAGA TCTGCCGCCG 
     1021 CGATCGCCAT GAGAGGCGCT CGCGGCGCCT GGGATTTTCT CTGCGTTCTG CTCCTACTGC 
     1081 TTCGCGTCCA GACAGGCTCT TCTCAACCAT CTGTGAGTCC AGGGGAACCG TCTCCACCAT 
     1141 CCATCCATCC AGGAAAATCA GACTTAATAG TCCGCGTGGG CGACGAGATT AGGCTGTTAT 
     1201 GCACTGATCC GGGCTTTGTC AAATGGACTT TTGAGATCCT GGATGAAACG AATGAGAATA 
     1261 AGCAGAATGA ATGGATCACG GAAAAGGCAG AAGCCACCAA CACCGGCAAA TACACGTGCA 
     1321 CCAACAAACA CGGCTTAAGC AATTCCATTT ATGTGTTTGT TAGAGATCCT GCCAAGCTTT 
     1381 TCCTTGTTGA CCGCTCCTTG TATGGGAAAG AAGACAACGA CACGCTGGTC CGCTGTCCTC 
     1441 TCACAGACCC AGAAGTGACC AATTATTCCC TCAAGGGGTG CCAGGGGAAG CCTCTTCCCA 
     1501 AGGACTTGAG GTTTATTCCT GACCCCAAGG CGGGCATCAT GATCAAAAGT GTGAAACGCG 
     1561 CCTACCATCG GCTCTGTCTG CATTGTTCTG TGGACCAGGA GGGCAAGTCA GTGCTGTCGG 
     1621 AAAAATTCAT CCTGAAAGTG AGGCCAGCCT TCAAAGCTGT GCCTGTTGTG TCTGTGTCCA 
     1681 AAGCAAGCTA TCTTCTTAGG GAAGGGGAAG AATTCACAGT GACGTGCACA ATAAAAGATG 
     1741 TGTCTAGTTC TGTGTACTCA ACGTGGAAAA GAGAAAACAG TCAGACTAAA CTACAGGAGA 
     1801 AATATAATAG CTGGCATCAC GGTGACTTCA ATTATGAACG TCAGGCAACG TTGACTATCA 
     1861 GTTCAGCGAG AGTTAATGAT TCTGGAGTGT TCATGTGTTA TGCCAATAAT ACTTTTGGAT 
     1921 CAGCAAATGT CACAACAACC TTGGAAGTAG TAGATAAAGG ATTCATTAAT ATCTTCCCCA 
     1981 TGATAAACAC TACAGTATTT GTAAACGATG GAGAAAATGT AGATTTGATT GTTGAATATG 
     2041 AAGCATTCCC CAAACCTGAA CACCAGCAGT GGATCTATAT GAACAGAACC TTCACTGATA 
     2101 AATGGGAAGA TTATCCCAAG TCTGAGAATG AAAGTAATAT CAGATACGTA AGTGAACTTC 
     2161 ATCTAACGAG ATTAAAAGGC ACCGAAGGAG GCACTTACAC ATTCCTAGTG TCCAATTCTG 
     2221 ACGTCAATGC TGCCATAGCA TTTAATGTTT ATGTGAATAC AAAACCAGAA ATCCTGACTT 
     2281 ACGACAGGCT CGTGAATGGC ATGCTCCAAT GTGTGGCAGC AGGATTCCCA GAGCCCACAA 
     2341 TAGATTGGTA TTTTTGTCCA GGAACTGAGC AGAGATGCTC TGCTTCTGTA CTGCCAGTGG 
     2401 ATGTGCAGAC ACTAAACTCA TCTGGGCCAC CGTTTGGAAA GCTAGTGGTT CAGAGTTCTA 
     2461 TAGATTCTAG TGCATTCAAG CACAATGGCA CGGTTGAATG TAAGGCTTAC AACGATGTGG 
     2521 GCAAGACTTC TGCCTATTTT AACTTTGCAT TTAAAGAGCA AATCCATCCC CACACCCTGT 
     2581 TCACTCCTTT GCTGATTGGT TTCGTAATCG TAGCTGGCAT GATGTGCATT ATTGTGATGA 
     2641 TTCTGACCTA CAAATATTTA CAGAAACCCA TGTATGAAGT ACAGTGGAAG GTTGTTGAGG 
     2701 AGATAAATGG AAACAATTAT GTTTACATAG ACCCAACACA ACTTCCTTAT GATCACAAAT 
     2761 GGGAGTTTCC CAGAAACAGG CTGAGTTTTG GGAAAACCCT GGGTGCTGGA GCTTTCGGGA 
     2821 AGGTTGTTGA GGCAACTGCT TATGGCTTAA TTAAGTCAGA TGCGGCCATG ACTGTCGCTG 
     2881 TAAAGATGCT CAAGCCGAGT GCCCATTTGA CAGAACGGGA AGCCCTCATG TCTGAACTCA 
     2941 AAGTCCTGAG TTACCTTGGT AATCACATGA ATATTGTGAA TCTACTTGGA GCCTGCACCA 
     3001 TTGGAGGGCC CACCCTGGTC ATTACAGAAT ATTGTTGCTA TGGTGATCTT TTGAATTTTT 
     3061 TGAGAAGAAA ACGTGATTCA TTTATTTGTT CAAAGCAGGA AGATCATGCA GAAGCTGCAC 
     3121 TTTATAAGAA TCTTCTGCAT TCAAAGGAGT CTTCCTGCAG CGATAGTACT AATGAGTACA 
     3181 TGGACATGAA ACCTGGAGTT TCTTATGTTG TCCCAACCAA GGCCGACAAA AGGAGATCTG 
     3241 TGAGAATAGG CTCATACATA GAAAGAGATG TGACTCCCGC CATCATGGAG GATGACGAGT 
     3301 TGGCCCTAGA CTTAGAAGAC TTGCTGAGCT TTTCTTACCA GGTGGCAAAG GGCATGGCTT 
     3361 TCCTCGCCTC CAAGAATTGT ATTCACAGAG ACTTGGCAGC CAGAAATATC CTCCTTACTC 
     3421 ATGGTCGGAT CACAAAGATT TGTGATTTTG GTCTAGCCAG AGACATCAAG AATGATTCTA 
     3481 ATTATGTGGT TAAAGGAAAC GCTCGACTAC CTGTGAAGTG GATGGCACCT GAAAGCATTT 
     3541 TCAACTGTGT ATACACGTTT GAAAGTGACG TCTGGTCCTA TGGGATTTTT CTTTGGGAGC 
     3601 TGTTCTCTTT AGGAAGCAGC CCCTATCCTG GAATGCCGGT CGATTCTAAG TTCTACAAGA 
     3661 TGATCAAGGA AGGCTTCCGG ATGCTCAGCC CTGAACACGC ACCTGCTGAA ATGTATGACA 
     3721 TAATGAAGAC TTGCTGGGAT GCAGATCCCC TAAAAAGACC AACATTCAAG CAAATTGTTC 
     3781 AGCTAATTGA GAAGCAGATT TCAGAGAGCA CCAATCATAT TTACTCCAAC TTAGCAAACT 
     3841 GCAGCCCCAA CCGACAGAAG CCCGTGGTAG ACCATTCTGT GCGGATCAAT TCTGTCGGCA 
     3901 GCACCGCTTC CTCCTCCCAG CCTCTGCTTG TGCACGACGA TGTCACGCGT ACGCGGCCGC 
     3961 TCGAGCAGAA ACTCATCTCA GAAGAGGATC TGGCAGCAAA TGATATCCTG GATTACAAGG 
     4021 ATGACGACGA TAAGGTTTAA ACGGCCGGCC GCGGTCATAG CTGTTTCCTG AACAGATCCC 
     4081 GGGTGGCATC CCTGTGACCC CTCCCCAGTG CCTCTCCTGG CCCTGGAAGT TGCCACTCCA 
     4141 GTGCCCACCA GCCTTGTCCT AATAAAATTA AGTTGCATCA TTTTGTCTGA CTAGGTGTCC 
     4201 TTCTATAATA TTATGGGGTG GAGGGGGGTG GTATGGAGCA AGGGGCAAGT TGGGAAGACA 
     4261 ACCTGTAGGG CCTGCGGGGT CTATTGGGAA CCAAGCTGGA GTGCAGTGGC ACAATCTTGG 
     4321 CTCACTGCAA TCTCCGCCTC CTGGGTTCAA GCGATTCTCC TGCCTCAGCC TCCCGAGTTG 
     4381 TTGGGATTCC AGGCATGCAT GACCAGGCTC AGCTAATTTT TGTTTTTTTG GTAGAGACGG 
     4441 GGTTTCACCA TATTGGCCAG GCTGGTCTCC AACTCCTAAT CTCAGGTGAT CTACCCACCT 
     4501 TGGCCTCCCA AATTGCTGGG ATTACAGGCG TGAACCACTG CTCCCTTCCC TGTCCTTCTG 
     4561 ATTTTAAAAT AACTATACCA GCAGGAGGAC GTCCAGACAC AGCATAGGCT ACCTGGCCAT 
     4621 GCCCAACCGG TGGGACATTT GAGTTGCTTG CTTGGCACTG TCCTCTCATG CGTTGGGTCC 
     4681 ACTCAGTAGA TGCCTGTTGA ATTGGGTACG CGGCCAGCGG CGAGCGGTAT CAGCTCACTC 
     4741 AAAGGCGGTA ATACGGTTAT CCACAGAATC AGGGGATAAC GCAGGAAAGA ACATGTGAGC 
     4801 AAAAGGCCAG CAAAAGGCCA GGAACCGTAA AAAGGCCGCG TTGCTGGCGT TTTTCCATAG 
     4861 GCTCCGCCCC CCTGACGAGC ATCACAAAAA TCGACGCTCA AGTCAGAGGT GGCGAAACCC 
     4921 GACAGGACTA TAAAGATACC AGGCGTTTCC CCCTGGAAGC TCCCTCGTGC GCTCTCCTGT 
     4981 TCCGACCCTG CCGCTTACCG GATACCTGTC CGCCTTTCTC CCTTCGGGAA GCGTGGCGCT 
     5041 TTCTCATAGC TCACGCTGTA GGTATCTCAG TTCGGTGTAG GTCGTTCGCT CCAAGCTGGG 
     5101 CTGTGTGCAC GAACCCCCCG TTCAGCCCGA CCGCTGCGCC TTATCCGGTA ACTATCGTCT 
     5161 TGAGTCCAAC CCGGTAAGAC ACGACTTATC GCCACTGGCA GCAGCCACTG GTAACAGGAT 
     5221 TAGCAGAGCG AGGTATGTAG GCGGTGCTAC AGAGTTCTTG AAGTGGTGGC CTAACTACGG 
     5281 CTACACTAGA AGAACAGTAT TTGGTATCTG CGCTCTGCTG AAGCCAGTTA CCTTCGGAAA 
     5341 AAGAGTTGGT AGCTCTTGAT CCGGCAAACA AACCACCGCT GGTAGCGGTG GTTTTTTTGT 
     5401 TTGCAAGCAG CAGATTACGC GCAGAAAAAA AGGATCTCAA GAAGATCCTT TGATCTTTTC 
     5461 TACGGGGTCT GACGCTCAGT GGAACGAAAA CTCACGTTAA GGGATTTTGG TCATGAGATT 
     5521 ATCAAAAAGG ATCTTCACCT AGATCCTTTT AAATTAAAAA TGAAGTTTTA AATCAATCTA 
     5581 AAGTATATAT GAGTAACCTG AGGCTATGGC AGGGCCTGCC GCCCCGACGT TGGCTGCGAG 
     5641 CCCTGGGCCT TCACCCGAAC TTGGGGGGTG GGGTGGGGAA AAGGAAGAAA CGCGGGCGTA 
     5701 TTGGCCCCAA TGGGGTCTCG GTGGGGTATC GACAGAGTGC CAGCCCTGGG ACCGAACCCC 
     5761 GCGTTTATGA ACAAACGACC CAACACCGTG CGTTTTATTC TGTCTTTTTA TTGCCGTCAT 
     5821 AGCGCGGGTT CCTTCCGGTA TTGTCTCCTT CCGTGTTTCA GTTAGCCTCC CCCTAGGGTG 
     5881 GGCGAAGAAC TCCAGCATGA GATCCCCGCG CTGGAGGATC ATCCAGCCGG CGTCCCGGAA 
     5941 AACGATTCCG AAGCCCAACC TTTCATAGAA GGCGGCGGTG GAATCGAAAT CTCGTGATGG 
     6001 CAGGTTGGGC GTCGCTTGGT CGGTCATTTC GAACCCCAGA GTCCCGCTCA GAAGAACTCG 
     6061 TCAAGAAGGC GATAGAAGGC GATGCGCTGC GAATCGGGAG CGGCGATACC GTAAAGCACG 
     6121 AGGAAGCGGT CAGCCCATTC GCCGCCAAGC TCTTCAGCAA TATCACGGGT AGCCAACGCT 
     6181 ATGTCCTGAT AGCGATCCGC CACACCCAGC CGGCCACAGT CGATGAATCC AGAAAAGCGG 
     6241 CCATTTTCCA CCATGATATT CGGCAAGCAG GCATCGCCAT GGGTCACGAC GAGATCCTCG 
     6301 CCGTCGGGCA TGCTCGCCTT GAGCCTGGCG AACAGTTCGG CTGGCGCGAG CCCCTGATGC 
     6361 TCTTCGTCCA GATCATCCTG ATCGACAAGA CCGGCTTCCA TCCGAGTACG TGCTCGCTCG 
     6421 ATGCGATGTT TCGCTTGGTG GTCGAATGGG CAGGTAGCCG GATCAAGCGT ATGCAGCCGC 
     6481 CGCATTGCAT CAGCCATGAT GGATACTTTC TCGGCAGGAG CAAGGTGAGA TGACAGGAGA 
     6541 TCCTGCCCCG GCACTTCGCC CAATAGCAGC CAGTCCCTTC CCGCTTCAGT GACAACGTCG 
     6601 AGCACAGCTG CGCAAGGAAC GCCCGTCGTG GCCAGCCACG ATAGCCGCGC TGCCTCGTCT 
     6661 TGCAGTTCAT TCAGGGCACC GGACAGGTCG GTCTTGACAA AAAGAACCGG GCGCCCCTGC 
     6721 GCTGACAGCC GGAACACGGC GGCATCAGAG CAGCCGATTG TCTGTTGTGC CCAGTCATAG 
     6781 CCGAATAGCC TCTCCACCCA AGCGGCCGGA GAACCTGCGT GCAATCCATC TTGTTCAATC 
     6841 ATGCGAAACG ATCCTCATCC TGTCTCTTGA TCGATCTTTG CAAAAGCCTA GGCCTCCAAA 
     6901 AAAGCCTCCT CACTACTTCT GGAATAGCTC AGAGGCCGAG GCGGCCTCGG CCTCTGCATA 
     6961 AATAAAAAAA ATTAGTCAGC CATGGGGCGG AGAATGGGCG GAACTGGGCG GAGTTAGGGG 
     7021 CGGGATGGGC GGAGTTAGGG GCGGGACTAT GGTTGCTGAC TAATTGAGAT GCATGCTTTG 
     7081 CATACTTCTG CCTGCTGGGG AGCCTGGGGA CTTTCCACAC CTGGTTGCTG ACTAATTGAG 
     7141 ATGCATGCTT TGCATACTTC TGCCTGCTGG GGAGCCTGGG GACTTTCCAC ACCCTAACTG 
     7201 ACACACATTC CACAGCTGGT TCTTTCCGCC TCAGGACTCT TCCTTTTTCA ATATTATTGA 
     7261 AGCATTTATC AGGGTTATTG TCTCATGAGC GGATACATAT TTGAATGTAT TTAGAAAAAT 
     7321 AAACAAATAG GGGTTCCGCG CACATTTCCC CGAAAAGTGC CACCTGACGC GCCCTGTAGC 
     7381 GGCGCATTAA GCGCGGCGGG TGTGGTGGTT ACGCGCAGCG TGACCGCTAC ACTTGCCAGC 
     7441 GCCCTAGCGC CCGCTCCTTT CGCTTTCTTC CCTTCCTTTC TCGCCACGTT CGCCGGCTTT 
     7501 CCCCGTCAAG CTCTAAATCG GGGGCTCCCT TTAGGGTTCC GATTTAGTGC TTTACGGCAC 
     7561 CTCGACCCCA AAAAACTTGA TTAGGGTGAT GGTTCACGTA GTGGGCCATC GCCCTGATAG 
     7621 ACGGTTTTTC GCCCTTTGAC GTTGGAGTCC ACGTTCTTTA ATAGTGGACT CTTGTTCCAA 
     7681 ACTGGAACAA CACTCAACCC TATCTCGGTC TATTCTTTTG ATTTATAAGG GATTTTGCCG 
     7741 ATTTCGGCCT ATTGGTTAAA AAATGAGCTG ATTTAACAAA AATTTAACGC GAATTTT
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