LOCUS       RC213623L1                9483 bp    DNA     circular  SYN 
DEFINITION  NLRP3 (Myc-DDK-tagged)-Human NLR family, pyrin domain containing 3 (NLRP3), transcript variant 3
ACCESSION   NM_001079821
VERSION     null
SOURCE      synthetic construct 
FEATURES    Location/Qualifiers 
     misc_feature    7774..7954 
                     /label="3'LTR"  
     misc_feature    833..1013 
                     /label="5'LTR"  
     misc_feature    8682..9341 
                     /label="CAT(Cam-r)"  
     misc_feature    2620..3234 
                     /label="CMV promoter"  
     misc_feature    8113..8681 
                     /label="ColE1"  
     misc_feature    6649..6669 
                     /label="LR50 primer"  
     misc_feature    6446..6517 
                     /label="Myc-DDK"  
     misc_feature    3252..3271 
                     /label="T7 promoter"  
     misc_feature    3221..3237 
                     /label="V2 primer"  
     misc_feature    6658..7249 
                     /label="WPRE"  
     misc_feature    3320..3328 
                     /label="Restriction Enzyme"  
     misc_feature    6428..6434 
                     /label="Restriction Enzyme"  
     misc_feature    3320..6428 
                     /label="NLRP3"  
ORIGIN                      
        1 GTCGACGGAT CGGGAGATCT CCCGATCCCC TATGGTGCAC TCTCAGTACA ATCTGCTCTG 
       61 ATGCCGCATA GTTAAGCCAG TATCTGCTCC CTGCTTGTGT GTTGGAGGTC GCTGAGTAGT 
      121 GCGCGAGCAA AATTTAAGCT ACAACAAGGC AAGGCTTGAC CGACAATTGC ATGAAGAATC 
      181 TGCTTAGGGT TAGGCGTTTT GCGCTGCTTC GCGATGTACG GGCCAGATAT CGCGTTGACA 
      241 TTGATTATTG ACTAGTTATT AATAGTAATC AATTACGGGG TCATTAGTTC ATAGCCCATA 
      301 TATGGAGTTC CGCGTTACAT AACTTACGGT AAATGGCCCG CCTGGCTGAC CGCCCAACGA 
      361 CCCCCGCCCA TTGACGTCAA TAATGACGTA TGTTCCCATA GTAACGCCAA TAGGGACTTT 
      421 CCATTGACGT CAATGGGTGG AGTATTTACG GTAAACTGCC CACTTGGCAG TACATCAAGT 
      481 GTATCATATG CCAAGTACGC CCCCTATTGA CGTCAATGAC GGTAAATGGC CCGCCTGGCA 
      541 TTATGCCCAG TACATGACCT TATGGGACTT TCCTACTTGG CAGTACATCT ACGTATTAGT 
      601 CATCGCTATT ACCATGGTGA TGCGGTTTTG GCAGTACATC AATGGGCGTG GATAGCGGTT 
      661 TGACTCACGG GGATTTCCAA GTCTCCACCC CATTGACGTC AATGGGAGTT TGTTTTGGCA 
      721 CCAAAATCAA CGGGACTTTC CAAAATGTCG TAACAACTCC GCCCCATTGA CGCAAATGGG 
      781 CGGTAGGCGT GTACGGTGGG AGGTCTATAT AAGCAGCGCG TTTTGCCTGT ACTGGGTCTC 
      841 TCTGGTTAGA CCAGATCTGA GCCTGGGAGC TCTCTGGCTA ACTAGGGAAC CCACTGCTTA 
      901 AGCCTCAATA AAGCTTGCCT TGAGTGCTTC AAGTAGTGTG TGCCCGTCTG TTGTGTGACT 
      961 CTGGTAACTA GAGATCCCTC AGACCCTTTT AGTCAGTGTG GAAAATCTCT AGCAGTGGCG 
     1021 CCCGAACAGG GACTTGAAAG CGAAAGGGAA ACCAGAGGAG CTCTCTCGAC GCAGGACTCG 
     1081 GCTTGCTGAA GCGCGCACGG CAAGAGGCGA GGGGCGGCGA CTGGTGAGTA CGCCAAAAAT 
     1141 TTTGACTAGC GGAGGCTAGA AGGAGAGAGA TGGGTGCGAG AGCGTCAGTA TTAAGCGGGG 
     1201 GAGAATTAGA TCGCGATGGG AAAAAATTCG GTTAAGGCCA GGGGGAAAGA AAAAATATAA 
     1261 ATTAAAACAT ATAGTATGGG CAAGCAGGGA GCTAGAACGA TTCGCAGTTA ATCCTGGCCT 
     1321 GTTAGAAACA TCAGAAGGCT GTAGACAAAT ACTGGGACAG CTACAACCAT CCCTTCAGAC 
     1381 AGGATCAGAA GAACTTAGAT CATTATATAA TACAGTAGCA ACCCTCTATT GTGTGCATCA 
     1441 AAGGATAGAG ATAAAAGACA CCAAGGAAGC TTTAGACAAG ATAGAGGAAG AGCAAAACAA 
     1501 AAGTAAGACC ACCGCACAGC AAGCGGCCGG CCGCTGATCT TCAGACCTGG AGGAGGAGAT 
     1561 ATGAGGGACA ATTGGAGAAG TGAATTATAT AAATATAAAG TAGTAAAAAT TGAACCATTA 
     1621 GGAGTAGCAC CCACCAAGGC AAAGAGAAGA GTGGTGCAGA GAGAAAAAAG AGCAGTGGGA 
     1681 ATAGGAGCTT TGTTCCTTGG GTTCTTGGGA GCAGCAGGAA GCACTATGGG CGCAGCGTCA 
     1741 ATGACGCTGA CGGTACAGGC CAGACAATTA TTGTCTGGTA TAGTGCAGCA GCAGAACAAT 
     1801 TTGCTGAGGG CTATTGAGGC GCAACAGCAT CTGTTGCAAC TCACAGTCTG GGGCATCAAG 
     1861 CAGCTCCAGG CAAGAATCCT GGCTGTGGAA AGATACCTAA AGGATCAACA GCTCCTGGGG 
     1921 ATTTGGGGTT GCTCTGGAAA ACTCATTTGC ACCACTGCTG TGCCTTGGAA TGCTAGTTGG 
     1981 AGTAATAAAT CTCTGGAACA GATTTGGAAT CACACGACCT GGATGGAGTG GGACAGAGAA 
     2041 ATTAACAATT ACACAAGCTT AATACACTCC TTAATTGAAG AATCGCAAAA CCAGCAAGAA 
     2101 AAGAATGAAC AAGAATTATT GGAATTAGAT AAATGGGCAA GTTTGTGGAA TTGGTTTAAC 
     2161 ATAACAAATT GGCTGTGGTA TATAAAATTA TTCATAATGA TAGTAGGAGG CTTGGTAGGT 
     2221 TTAAGAATAG TTTTTGCTGT ACTTTCTATA GTGAATAGAG TTAGGCAGGG ATATTCACCA 
     2281 TTATCGTTTC AGACCCACCT CCCAACCCCG AGGGGACCCG ACAGGCCCGA AGGAATAGAA 
     2341 GAAGAAGGTG GAGAGAGAGA CAGAGACAGA TCCATTCGAT TAGTGAACGG ATCGGCACTG 
     2401 CGTGCGCCAA TTCTGCAGAC AAATGGCAGT ATTCATCCAC AATTTTAAAA GAAAAGGGGG 
     2461 GATTGGGGGG TACAGTGCAG GGGAAAGAAT AGTAGACATA ATAGCAACAG ACATACAAAC 
     2521 TAAAGAATTA CAAAAACAAA TTACAAAAAT TCAAAATTTT CGGGTTTATT ACAGGGACAG 
     2581 CAGAGATCCA GTTTGGTTAG TACCGGGCCC GCTCTAGACA TGTCCAATAT GACCGCCATG 
     2641 TTGACATTGA TTATTGACTA GTTATTAATA GTAATCAATT ACGGGGTCAT TAGTTCATAG 
     2701 CCCATATATG GAGTTCCGCG TTACATAACT TACGGTAAAT GGCCCGCCTG GCTGACCGCC 
     2761 CAACGACCCC CGCCCATTGA CGTCAATAAT GACGTATGTT CCCATAGTAA CGCCAATAGG 
     2821 GACTTTCCAT TGACGTCAAT GGGTGGAGTA TTTACGGTAA ACTGCCCACT TGGCAGTACA 
     2881 TCAAGTGTAT CATATGCCAA GTCCGCCCCC TATTGACGTC AATGACGGTA AATGGCCCGC 
     2941 CTGGCATTAT GCCCAGTACA TGACCTTACG GGACTTTCCT ACTTGGCAGT ACATCTACGT 
     3001 ATTAGTCATC GCTATTACCA TGGTGATGCG GTTTTGGCAG TACACCAATG GGCGTGGATA 
     3061 GCGGTTTGAC TCACGGGGAT TTCCAAGTCT CCACCCCATT GACGTCAATG GGAGTTTGTT 
     3121 TTGGCACCAA AATCAACGGG ACTTTCCAAA ATGTCGTAAT AACCCCGCCC CGTTGACGCA 
     3181 AATGGGCGGT AGGCGTGTAC GGTGGGAGGT CTATATAAGC AGAGCTCGTT TAGTGAACCG 
     3241 TCAGAATTTT GTAATACGAC TCACTATAGG GCGGCCGGGA ATTCGTCGAC TGGATCCGGT 
     3301 ACCGAGGAGA TCTGCCGCCG CGATCGCCAT GAAGATGGCA AGCACCCGCT GCAAGCTGGC 
     3361 CAGGTACCTG GAGGACCTGG AGGATGTGGA CTTGAAGAAA TTTAAGATGC ACTTAGAGGA 
     3421 CTATCCTCCC CAGAAGGGCT GCATCCCCCT CCCGAGGGGT CAGACAGAGA AGGCAGACCA 
     3481 TGTGGATCTA GCCACGCTAA TGATCGACTT CAATGGGGAG GAGAAGGCGT GGGCCATGGC 
     3541 CGTGTGGATC TTCGCTGCGA TCAACAGGAG AGACCTTTAT GAGAAAGCAA AAAGAGATGA 
     3601 GCCGAAGTGG GGTTCAGATA ATGCACGTGT TTCGAATCCC ACTGTGATAT GCCAGGAAGA 
     3661 CAGCATTGAA GAGGAGTGGA TGGGTTTACT GGAGTACCTT TCGAGAATCT CTATTTGTAA 
     3721 AATGAAGAAA GATTACCGTA AGAAGTACAG AAAGTACGTG AGAAGCAGAT TCCAGTGCAT 
     3781 TGAAGACAGG AATGCCCGTC TGGGTGAGAG TGTGAGCCTC AACAAACGCT ACACACGACT 
     3841 GCGTCTCATC AAGGAGCACC GGAGCCAGCA GGAGAGGGAG CAGGAGCTTC TGGCCATCGG 
     3901 CAAGACCAAG ACGTGTGAGA GCCCCGTGAG TCCCATTAAG ATGGAGTTGC TGTTTGACCC 
     3961 CGATGATGAG CATTCTGAGC CTGTGCACAC CGTGGTGTTC CAGGGGGCGG CAGGGATTGG 
     4021 GAAAACAATC CTGGCCAGGA AGATGATGTT GGACTGGGCG TCGGGGACAC TCTACCAAGA 
     4081 CAGGTTTGAC TATCTGTTCT ATATCCACTG TCGAGAGGTG AGCCTTGTGA CACAGAGGAG 
     4141 CCTGGGGGAC CTGATCATGA GCTGCTGCCC CGACCCAAAC CCACCCATCC ACAAGATCGT 
     4201 GAGAAAACCC TCCAGAATCC TCTTCCTCAT GGACGGCTTC GATGAGCTGC AAGGTGCCTT 
     4261 TGACGAGCAC ATAGGACCGC TCTGCACTGA CTGGCAGAAG GCCGAGCGGG GAGACATTCT 
     4321 CCTGAGCAGC CTCATCAGAA AGAAGCTGCT TCCCGAGGCC TCTCTGCTCA TCACCACGAG 
     4381 ACCTGTGGCC CTGGAGAAAC TGCAGCACTT GCTGGACCAT CCTCGGCATG TGGAGATCCT 
     4441 GGGTTTCTCC GAGGCCAAAA GGAAAGAGTA CTTCTTCAAG TACTTCTCTG ATGAGGCCCA 
     4501 AGCCAGGGCA GCCTTCAGTC TGATTCAGGA GAACGAGGTC CTCTTCACCA TGTGCTTCAT 
     4561 CCCCCTGGTC TGCTGGATCG TGTGCACTGG ACTGAAACAG CAGATGGAGA GTGGCAAGAG 
     4621 CCTTGCCCAG ACATCCAAGA CCACCACCGC GGTGTACGTC TTCTTCCTTT CCAGTTTGCT 
     4681 GCAGCCCCGG GGAGGGAGCC AGGAGCACGG CCTCTGCGCC CACCTCTGGG GGCTCTGCTC 
     4741 TTTGGCTGCA GATGGAATCT GGAACCAGAA AATCCTGTTT GAGGAGTCCG ACCTCAGGAA 
     4801 TCATGGACTG CAGAAGGCGG ATGTGTCTGC TTTCCTGAGG ATGAACCTGT TCCAAAAGGA 
     4861 AGTGGACTGC GAGAAGTTCT ACAGCTTCAT CCACATGACT TTCCAGGAGT TCTTTGCCGC 
     4921 CATGTACTAC CTGCTGGAAG AGGAAAAGGA AGGAAGGACG AACGTTCCAG GGAGTCGTTT 
     4981 GAAGCTTCCC AGCCGAGACG TGACAGTCCT TCTGGAAAAC TATGGCAAAT TCGAAAAGGG 
     5041 GTATTTGATT TTTGTTGTAC GTTTCCTCTT TGGCCTGGTA AACCAGGAGA GGACCTCCTA 
     5101 CTTGGAGAAG AAATTAAGTT GCAAGATCTC TCAGCAAATC AGGCTGGAGC TGCTGAAATG 
     5161 GATTGAAGTG AAAGCCAAAG CTAAAAAGCT GCAGATCCAG CCCAGCCAGC TGGAATTGTT 
     5221 CTACTGTTTG TACGAGATGC AGGAGGAGGA CTTCGTGCAA AGGGCCATGG ACTATTTCCC 
     5281 CAAGATTGAG ATCAATCTCT CCACCAGAAT GGACCACATG GTTTCTTCCT TTTGCATTGA 
     5341 GAACTGTCAT CGGGTGGAGT CACTGTCCCT GGGGTTTCTC CATAACATGC CCAAGGAGGA 
     5401 AGAGGAGGAG GAAAAGGAAG GCCGACACCT TGATATGGTG CAGTGTGTCC TCCCAAGCTC 
     5461 CTCTCATGCT GCCTGTTCTC ATGGATTGGT GAACAGCCAC CTCACTTCCA GTTTTTGCCG 
     5521 GGGCCTCTTT TCAGTTCTGA GCACCAGCCA GAGTCTAACT GAATTGGACC TCAGTGACAA 
     5581 TTCTCTGGGG GACCCAGGGA TGAGAGTGTT GTGTGAAACG CTCCAGCATC CTGGCTGTAA 
     5641 CATTCGGAGA TTGTGGTTGG GGCGCTGTGG CCTCTCGCAT GAGTGCTGCT TCGACATCTC 
     5701 CTTGGTCCTC AGCAGCAACC AGAAGCTGGT GGAGCTGGAC CTGAGTGACA ACGCCCTCGG 
     5761 TGACTTCGGA ATCAGACTTC TGTGTGTGGG ACTGAAGCAC CTGTTGTGCA ATCTGAAGAA 
     5821 GCTCTGGTTG GTCAGCTGCT GCCTCACATC AGCATGTTGT CAGGATCTTG CATCAGTATT 
     5881 GAGCACCAGC CATTCCCTGA CCAGACTCTA TGTGGGGGAG AATGCCTTGG GAGACTCAGG 
     5941 AGTCGCAATT TTATGTGAAA AAGCCAAGAA TCCACAGTGT AACCTGCAGA AACTGGGGTT 
     6001 GGTGAATTCT GGCCTTACGT CAGTCTGTTG TTCAGCTTTG TCCTCGGTAC TCAGCACTAA 
     6061 TCAGAATCTC ACGCACCTTT ACCTGCGAGG CAACACTCTC GGAGACAAGG GGATCAAACT 
     6121 ACTCTGTGAG GGACTCTTGC ACCCCGACTG CAAGCTTCAG GTGTTGGAAT TAGACAACTG 
     6181 CAACCTCACG TCACACTGCT GCTGGGATCT TTCCACACTT CTGACCTCCA GCCAGAGCCT 
     6241 GCGAAAGCTG AGCCTGGGCA ACAATGACCT GGGCGACCTG GGGGTCATGA TGTTCTGTGA 
     6301 AGTGCTGAAA CAGCAGAGCT GCCTCCTGCA GAACCTGGGG TTGTCTGAAA TGTATTTCAA 
     6361 TTATGAGACA AAAAGTGCGT TAGAAACACT TCAAGAAGAA AAGCCTGAGC TGACCGTCGT 
     6421 CTTTGAGCCT TCTTGGACGC GTACGCGGCC GCTCGAGCAG AAACTCATCT CAGAAGAGGA 
     6481 TCTGGCAGCA AATGATATCC TGGATTACAA GGATGACGAC GATAAGGTTT AAACGGCCGG 
     6541 CCGCGGTCTG TACAAGTAGG ATTCGTCGAG GGACCTAATA ACTTCGTATA GCATACATTA 
     6601 TACGAAGTTA TACATGTTTA AGGGTTCCGG TTCCACTAGG TACAATTCGA TATCAAGCTT 
     6661 ATCGATAATC AACCTCTGGA TTACAAAATT TGTGAAAGAT TGACTGGTAT TCTTAACTAT 
     6721 GTTGCTCCTT TTACGCTATG TGGATACGCT GCTTTAATGC CTTTGTATCA TGCTATTGCT 
     6781 TCCCGTATGG CTTTCATTTT CTCCTCCTTG TATAAATCCT GGTTGCTGTC TCTTTATGAG 
     6841 GAGTTGTGGC CCGTTGTCAG GCAACGTGGC GTGGTGTGCA CTGTGTTTGC TGACGCAACC 
     6901 CCCACTGGTT GGGGCATTGC CACCACCTGT CAGCTCCTTT CCGGGACTTT CGCTTTCCCC 
     6961 CTCCCTATTG CCACGGCGGA ACTCATCGCC GCCTGCCTTG CCCGCTGCTG GACAGGGGCT 
     7021 CGGCTGTTGG GCACTGACAA TTCCGTGGTG TTGTCGGGGA AATCATCGTC CTTTCCTTGG 
     7081 CTGCTCGCCT GTGTTGCCAC CTGGATTCTG CGCGGGACGT CCTTCTGCTA CGTCCCTTCG 
     7141 GCCCTCAATC CAGCGGACCT TCCTTCCCGC GGCCTGCTGC CGGCTCTGCG GCCTCTTCCG 
     7201 CGTCTTCGCC TTCGCCCTCA GACGAGTCGG ATCTCCCTTT GGGCCGCCTC CCCGCATCGA 
     7261 TACCGTCGAC CTCGATCGAG ACCTAGAAAA ACATGGAGCA ATCACAAGTA GCAATACAGC 
     7321 AGCTACCAAT GCTGATTGTG CCTGGCTAGA AGCACAAGAG GAGGAGGAGG TGGGTTTTCC 
     7381 AGTCACACCT CAGGTACCTT TAAGACCAAT GACTTACAAG GCAGCTGTAG ATCTTAGCCA 
     7441 CTTTTTAAAA GAAAAGGGGG GACTGGAAGG GCTAATTCAC TCCCAACGAA GACAAGATAT 
     7501 CCTTGATCTG TGGATCTACC ACACACAAGG CTACTTCCCT GATTGGCAGA ACTACACACC 
     7561 AGGGCCAGGG ATCAGATATC CACTGACCTT TGGATGGTGC TACAAGCTAG TACCAGTTGA 
     7621 GCAAGAGAAG GTAGAAGAAG CCAATGAAGG AGAGAACACC CGCTTGTTAC ACCCTGTGAG 
     7681 CCTGCATGGG ATGGATGACC CGGAGAGAGA AGTATTAGAG TGGAGGTTTG ACAGCCGCCT 
     7741 AGCATTTCAT CACATGGCCC GAGAGCTGCA TCCGGACTGT ACTGGGTCTC TCTGGTTAGA 
     7801 CCAGATCTGA GCCTGGGAGC TCTCTGGCTA ACTAGGGAAC CCACTGCTTA AGCCTCAATA 
     7861 AAGCTTGCCT TGAGTGCTTC AAGTAGTGTG TGCCCGTCTG TTGTGTGACT CTGGTAACTA 
     7921 GAGATCCCTC AGACCCTTTT AGTCAGTGTG GAAAATCTCT AGCAGCATGT GAGCAAAAGG 
     7981 CCAGCAAAAG GCCAGGAACC GTAAAAAGGC CGCGTTGCTG GCGTTTTTCC ATAGGCTCCG 
     8041 CCCCCCTGAC GAGCATCACA AAAATCGACG CTCAAGTCAG AGGTGGCGAA ACCCGACAGG 
     8101 ACTATAAAGA TACCAGGCGT TTCCCCCTGG AAGCTCCCTC GTGCGCTCTC CTGTTCCGAC 
     8161 CCTGCCGCTT ACCGGATACC TGTCCGCCTT TCTCCCTTCG GGAAGCGTGG CGCTTTCTCA 
     8221 TAGCTCACGC TGTAGGTATC TCAGTTCGGT GTAGGTCGTT CGCTCCAAGC TGGGCTGTGT 
     8281 GCACGAACCC CCCGTTCAGC CCGACCGCTG CGCCTTATCC GGTAACTATC GTCTTGAGTC 
     8341 CAACCCGGTA AGACACGACT TATCGCCACT GGCAGCAGCC ACTGGTAACA GGATTAGCAG 
     8401 AGCGAGGTAT GTAGGCGGTG CTACAGAGTT CTTGAAGTGG TGGCCTAACT ACGGCTACAC 
     8461 TAGAAGAACA GTATTTGGTA TCTGCGCTCT GCTGAAGCCA GTTACCTTCG GAAAAAGAGT 
     8521 TGGTAGCTCT TGATCCGGCA AACAAACCAC CGCTGGTAGC GGTGGTTTTT TTGTTTGCAA 
     8581 GCAGCAGATT ACGCGCAGAA AAAAAGGATC TCAAGAAGAT CCTTTGATCT TTTCTACGGG 
     8641 GTCTGACGCT CAGTGGAACG AAAACTCACG TTAAGGGATT TTGGTCATGA TTACGCCCCG 
     8701 CCCTGCCACT CATCGCAGTA CTGTTGTAAT TCATTAAGCA TTCTGCCGAC ATGGAAGCCA 
     8761 TCACAAACGG CATGATGAAC CTGAATCGCC AGCGGCATCA GCACCTTGTC GCCTTGCGTA 
     8821 TAATATTTGC CCATGGTGAA AACGGGGGCG AAGAAGTTGT CCATATTGGC CACGTTTAAA 
     8881 TCAAAACTGG TGAAACTCAC CCAGGGATTG GCTGAGACGA AAAACATATT CTCAATAAAC 
     8941 CCTTTAGGGA AATAGGCCAG GTTTTCACCG TAACACGCCA CATCTTGCGA ATATATGTGT 
     9001 AGAAACTGCC GGAAATCGTC GTGGTATTCA CTCCAGAGCG ATGAAAACGT TTCAGTTTGC 
     9061 TCATGGAAAA CGGTGTAACA AGGGTGAACA CTATCCCATA TCACCAGCTC ACCGTCTTTC 
     9121 ATTGCCATAC GGAACTCCGG ATGAGCATTC ATCAGGCGGG CAAGAATGTG AATAAAGGCC 
     9181 GGATAAAACT TGTGCTTATT TTTCTTTACG GTCTTTAAAA AGGCCGTAAT ATCCAGCTGA 
     9241 ACGGTCTGGT TATAGGTACA TTGAGCAACT GACTGAAATG CCTCAAAATG TTCTTTACGA 
     9301 TGCCATTGGG ATATATCAAC GGTGGTATAT CCAGTGATTT TTTTCTCCAT ACTCTTCCTT 
     9361 TTTCAATATT ATTGAAGCAT TTATCAGGGT TATTGTCTCA TGAGCGGATA CATATTTGAA 
     9421 TGTATTTAGA AAAATAAACA AATAGGGGTC CCGCGCACAT TTCCCCGAAA AGTGCCACCT 
     9481 GAC
//