LOCUS       RC213622                8757 bp    DNA     circular  SYN 
DEFINITION  SORBS1 (Myc-DDK-tagged)-Human sorbin and SH3 domain containing 1 (SORBS1), transcript variant 3
ACCESSION   NM_001034954
VERSION     NM_001034954.1
SOURCE      synthetic construct 
FEATURES    Location/Qualifiers 
     misc_feature    201..924 
                     /label="CMV promoter"  
     misc_feature    839..856 
                     /label="Restriction Enzyme"  
     misc_feature    953..971 
                     /label="T7 promoter"  
     misc_feature    4914..5005 
                     /label="Myc-DDK"  
     misc_feature    5035..5620 
                     /label="PolyA signal"  
     misc_feature    5092..5111 
                     /label="Restriction Enzyme"  
     misc_feature    5896..6568 
                     /label="ColE1"  
     misc_feature    6998..7792 
                     /label="Kan/Neo"  
     misc_feature    7827..8129 
                     /label="SV40 ori"  
     misc_feature    8215..8692 
                     /label="f1 ori"  
     misc_feature    1020..1028 
                     /label="Restriction Enzyme"  
     misc_feature    4896..4902 
                     /label="Restriction Enzyme"  
     misc_feature    1020..4896 
                     /label="SORBS1"  
ORIGIN                      
        1 AACAAAATAT TAACGCTTAC AATTTCCATT CGCCATTCAG GCTGCGCAAC TGTTGGGAAG 
       61 GGCGATCGGT GCGGGCCTCT TCGCTATTAC GCCAGCTGGC GAAAGGGGGA TGTGCTGCAA 
      121 GGCGATTAAG TTGGGTAACG CCAGGGTTTT CCCAGTCACG ACGTTGTAAA ACGACGGCCA 
      181 GTGCCAAGCT GATCTATACA TTGAATCAAT ATTGGCAATT AGCCATATTA GTCATTGGTT 
      241 ATATAGCATA AATCAATATT GGCTATTGGC CATTGCATAC GTTGTATCTA TATCATAATA 
      301 TGTACATTTA TATTGGCTCA TGTCCAATAT GACCGCCATG TTGACATTGA TTATTGACTA 
      361 GTTATTAATA GTAATCAATT ACGGGGTCAT TAGTTCATAG CCCATATATG GAGTTCCGCG 
      421 TTACATAACT TACGGTAAAT GGCCCGCCTG GCTGACCGCC CAACGACCCC CGCCCATTGA 
      481 CGTCAATAAT GACGTATGTT CCCATAGTAA CGCCAATAGG GACTTTCCAT TGACGTCAAT 
      541 GGGTGGAGTA TTTACGGTAA ACTGCCCACT TGGCAGTACA TCAAGTGTAT CATATGCCAA 
      601 GTCCGCCCCC TATTGACGTC AATGACGGTA AATGGCCCGC CTGGCATTAT GCCCAGTACA 
      661 TGACCTTACG GGACTTTCCT ACTTGGCAGT ACATCTACGT ATTAGTCATC GCTATTACCA 
      721 TGGTGATGCG GTTTTGGCAG TACACCAATG GGCGTGGATA GCGGTTTGAC TCACGGGGAT 
      781 TTCCAAGTCT CCACCCCATT GACGTCAATG GGAGTTTGTT TTGGCACCAA AATCAACGGG 
      841 ACTTTCCAAA ATGTCGTAAT AACCCCGCCC CGTTGACGCA AATGGGCGGT AGGCGTGTAC 
      901 GGTGGGAGGT CTATATAAGC AGAGCTCGTT TAGTGAACCG TCAGAATTTT GTAATACGAC 
      961 TCACTATAGG GCGGCCGGGA ATTCGTCGAC TGGATCCGGT ACCGAGGAGA TCTGCCGCCG 
     1021 CGATCGCCAT GAGTTCTGAA TGTGATGGTG GTTCCAAAGC TGTGATGAAT GGCTTGGCAC 
     1081 CTGGCAGCAA TGGGCAAGAC AAAGCAACTG CCGACCCTTT ACGCGCACGC TCTATTTCTG 
     1141 CTGTTAAAAT CATTCCTGTG AAGACAGTGA AAAACGCCTC AGGCCTAGTT CTCCCTACAG 
     1201 ACATGGATCC TACAAAAATC TGCACTGGGA AGGGAGCGGT GACTCTCCGG GCCTCGTCTT 
     1261 CCTACAGGGA AACCCCAAGC AGTAGCCCTG CGAGCCCTCA GGAAACCCGG CAACACGAAA 
     1321 GCAAACCAGG TCTGGAGCCA GAGCCTTCTT CAGCAGATGA GTGGAGGCTT TCTTCCAGTG 
     1381 CTGATGCCAA TGGAAATGCC CAGCCCTCTT CACTCGCTGC CAAGGGCTAC AGAAGTGTGC 
     1441 ATCCCAACCT TCCTTCTGAC AAGTCCCAGG ATGCCACTTC CTCCAGTGCA GCCCAGCCGG 
     1501 AGGTAATAGT TGTCCCTCTC TACCTGGTTA ATACTGACAG AGGGCAAGAA GGCACTGCCA 
     1561 GACCTCCAAC ACCTCTGGGG CCTCTTGGCT GCGTCCCCAC AATCCCAGCG ACTGCCTCTG 
     1621 CCGCCTCACC TCTGACCTTC CCGACTCTAG ATGATTTCAT TCCCCCTCAT CTGCAGAGGT 
     1681 GGCCCCACCA CAGCCAGCCA GCCCGCGCCT CTGGCTCCTT TGCCCCCATT AGCCAGACGC 
     1741 CACCATCCTT CTCACCACCA CCTCCGCTGG TCCCTCCTGC CCCGGAGGAC CTCCGCAGAG 
     1801 TCTCGGAGCC TGACCTCACG GGAGCTGTTT CGAGTACCGA TTCCAGTCCT CTACTAAATG 
     1861 AAGTTTCTTC TTCCCTTATT GGAACTGATT CCCAAGCCTT TCCATCAGTT AGCAAGCCTT 
     1921 CATCCGCCTA TCCCTCCACA ACGATTGTCA ATCCTACTAT TGTGCTCTTG CAACACAATC 
     1981 GAGAACAGCA AAAACGACTC AGTAGCCTTT CAGATCCTGT CTCAGAAAGA AGAGTGGGAG 
     2041 AGCAGGACTC AGCACCAACC CAGGAAAAAC CCACCTCACC TGGCAAGGCT ATTGAAAAAA 
     2101 GAGCAAAGGA TGACAGTAGG CGGGTGGTGA AGAGCACTCA GGACTTAAGC GATGTTTCCA 
     2161 TGGATGAAGT GGGCATCCCA CTCCGGAACA CTGAGAGATC AAAAGACTGG TACAAGACTA 
     2221 TGTTTAAACA GATCCACAAA CTGAACAGAG ACACTCCTGA AGAAAACCCT TATTTCCCTA 
     2281 CGTACAAATT CCCTGAACTT CCTGAAATCC AGCAAACTTC CGAAGAGGAC AATCCTTACA 
     2341 CTCCCACCTA CCAGTTTCCT GCATCTACTC CTAGTCCTAA ATCTGAAGAT GATGATTCAG 
     2401 ATCTGTACTC TCCCAGATAC TCATTTTCTG AAGACACAAA ATCTCCCCTT TCTGTGCCTC 
     2461 GCTCAAAAAG TGAGATGAGC TACATTGATG GTGAGAAGGT AGTCAAGAGG TCGGCCACAC 
     2521 TACCCCTCCC AGCCCGCTCT TCCTCACTGA AGTCAAGCTC AGAAAGAAAT GACTGGGAAC 
     2581 CCCCAGATAA GAAAGTAGAC ACAAGAAAAT ATCGTGCAGA GCCCAAGAGC ATTTACGAAT 
     2641 ATCAGCCTGG CAAGTCTTCC GTTCTGACCA ACGAAAAGAT GAGTCGGGAT ATAAGCCCAG 
     2701 AAGAGATAGA TTTAAAGAAT GAACCTTGGT ATAAATTCTT TTCGGAATTG GAGTTTGGGA 
     2761 AACCGCCTCC CAAAAAGATA TGGGATTATA CTCCTGGAGA CTGCTCTATC CTTCCTAGAG 
     2821 AGGATAGAAA GACTAATCTA GACAAAGATC TCAGCCTCTG CCAGACAGAG TTAGAGGCAG 
     2881 ATTTAGAAAA AATGGAGACG CTTAATAAAG CACCCAGTGC AAACGTGCCA CAGAGCTCAG 
     2941 CCATCAGCCC TACTCCGGAA ATTTCTTCAG AGACTCCTGG ATATATATAT TCTTCCAACT 
     3001 TCCATGCAGT GAAGAGGGAA TCAGACGGGG CTCCTGGGGA TCTCACTAGC TTGGAGAATG 
     3061 AGAGACAAAT TTATAAAAGT GTCTTGGAAG GTGGTGACAT CCCTCTTCAG GGCCTGAGTG 
     3121 GGCTCAAGCG ACCATCCAGC TCTGCTTCCA CTAAAGATTC AGAATCGCCA AGACATTTTA 
     3181 TACCAGCTGA TTACTTGGAA TCCACGGAAG AATTTATTCG AAGACGTCAT GATGATAAAG 
     3241 AGAAACTTTT AGCGGACCAG AGACGACTTA AACGCGAGCA AGAAGAGGCT GATATTGCAG 
     3301 CTCGACGCCA CACAGGCGTC ATTCCGACGC ACCATCAGTT TATCACTAAT GAGCGCTTTG 
     3361 GGGACCTCCT CAATATAGAC GATACTGCAA AAAGGAAATC TGGGTCAGAG ATGAGACCTG 
     3421 CCAGAGCCAA ATTTGACTTT AAAGCTCAGA CACTAAAGGA GCTTCCTCTG CAGAAGGGAG 
     3481 ATATTGTTTA CATTTATAAG CAAATTGATC AGAACTGGTA TGAAGGAGAA CACCACGGCC 
     3541 GGGTGGGAAT CTTCCCACGC ACCTACATCG AGCTTCTTCC TCCTGCTGAG AAGGCACAGC 
     3601 CCAAAAAGTT GACACCAGTG CAGGTTTTGG AATATGGAGA AGCTATTGCT AAGTTTAACT 
     3661 TTAATGGTGA TACACAAGTA GAAATGTCCT TCAGAAAGGG TGAGAGGATC ACACTGCTCC 
     3721 GGCAGGTAGA TGAGAACTGG TACGAAGGGA GGATCCCGGG GACATCCCGA CAAGGCATCT 
     3781 TCCCCATCAC CTACGTGGAT GTGATCAAGC GACCACTGGT GAAAAACCCT GTGGATTACA 
     3841 TGGACCTGCC TTTCTCCTCC TCCCCAAGTC GCAGTGCCAC TGCAAGCCCA CAGTTTTCCA 
     3901 GTCACAGCAA GCTCATCACG CCAGCCCCCT CATCTCTGCC CCACTCCCGC CGAGCCCTGT 
     3961 CCCCCGAGAT GCACGCTGTC ACCTCTGAGT GGATCTCACT GACTGTGGGG GTCCCAGGCA 
     4021 GGCGTTCTCT GGCCCTGACC CCACCCTTGC CTCCTCTGCC AGAGGCTTCT ATCTATAACA 
     4081 CTGACCACCT CGCCTTGTCA CCAAGGGCCA GTCCCTCCCT GTCTCTCAGC CTCCCCCATT 
     4141 TGAGTTGGTC AGATCGTCCC ACCCCACGAT CAGTAGCTTC TCCACTGGCC CTACCTTCCC 
     4201 CACACAAAAC CTACTCCCTA GCACCTACTT CCCAGGCCTC CCTTCACATG AATGGAGACG 
     4261 GTGGTGTCCA CACGCCATCT TCAGGCATCC ACCAAGATAG CTTCTTGCAG CTGCCGCTGG 
     4321 GGAGCTCTGA TAGTGTCATC TCCCAGCTTA GTGATGCCTT TAGCAGCCAG AGCAAGAGGC 
     4381 AGCCATGGCG CGAAGAGAGT GGACAATATG AGAGGAAAGC AGAGAGGGGG GCAGGCGAAA 
     4441 GAGGCCCTGG TGGACCCAAG ATCTCTAAGA AGAGCTGCTT GAAGCCTTCA GACGTGGTCA 
     4501 GGTGCCTGAG TACTGAACAG AGACTCTCAG ATCTCAACAC CCCTGAGGAG AGCCGGCCCG 
     4561 GCAAGCCCCT GGGTAGCGCT TTTCCAGGAA GTGAGGCTGA GCAGACAGAG CGGCATAGAG 
     4621 GTGGCGAGCA GGCGGGGAGG AAAGCTGCTC GGAGAGGTGG GAGCCAGCAA CCTCAAGCCC 
     4681 AGCAGCGAAG AGTCACCCCC GACAGGAGTC AAACCTCACA AGATTTATTT AGCTATCAAG 
     4741 CATTATATAG CTATATACCA CAGAATGATG ATGAGTTGGA ACTCCGCGAT GGAGATATCG 
     4801 TTGATGTCAT GGAAAAATGT GACGATGGAT GGTTTGTTGG TACTTCAAGA AGGACAAAGC 
     4861 AGTTTGGTAC TTTTCCAGGC AACTATGTAA AACCTTTGTA TCTAACGCGT ACGCGGCCGC 
     4921 TCGAGCAGAA ACTCATCTCA GAAGAGGATC TGGCAGCAAA TGATATCCTG GATTACAAGG 
     4981 ATGACGACGA TAAGGTTTAA ACGGCCGGCC GCGGTCATAG CTGTTTCCTG AACAGATCCC 
     5041 GGGTGGCATC CCTGTGACCC CTCCCCAGTG CCTCTCCTGG CCCTGGAAGT TGCCACTCCA 
     5101 GTGCCCACCA GCCTTGTCCT AATAAAATTA AGTTGCATCA TTTTGTCTGA CTAGGTGTCC 
     5161 TTCTATAATA TTATGGGGTG GAGGGGGGTG GTATGGAGCA AGGGGCAAGT TGGGAAGACA 
     5221 ACCTGTAGGG CCTGCGGGGT CTATTGGGAA CCAAGCTGGA GTGCAGTGGC ACAATCTTGG 
     5281 CTCACTGCAA TCTCCGCCTC CTGGGTTCAA GCGATTCTCC TGCCTCAGCC TCCCGAGTTG 
     5341 TTGGGATTCC AGGCATGCAT GACCAGGCTC AGCTAATTTT TGTTTTTTTG GTAGAGACGG 
     5401 GGTTTCACCA TATTGGCCAG GCTGGTCTCC AACTCCTAAT CTCAGGTGAT CTACCCACCT 
     5461 TGGCCTCCCA AATTGCTGGG ATTACAGGCG TGAACCACTG CTCCCTTCCC TGTCCTTCTG 
     5521 ATTTTAAAAT AACTATACCA GCAGGAGGAC GTCCAGACAC AGCATAGGCT ACCTGGCCAT 
     5581 GCCCAACCGG TGGGACATTT GAGTTGCTTG CTTGGCACTG TCCTCTCATG CGTTGGGTCC 
     5641 ACTCAGTAGA TGCCTGTTGA ATTGGGTACG CGGCCAGCGG CGAGCGGTAT CAGCTCACTC 
     5701 AAAGGCGGTA ATACGGTTAT CCACAGAATC AGGGGATAAC GCAGGAAAGA ACATGTGAGC 
     5761 AAAAGGCCAG CAAAAGGCCA GGAACCGTAA AAAGGCCGCG TTGCTGGCGT TTTTCCATAG 
     5821 GCTCCGCCCC CCTGACGAGC ATCACAAAAA TCGACGCTCA AGTCAGAGGT GGCGAAACCC 
     5881 GACAGGACTA TAAAGATACC AGGCGTTTCC CCCTGGAAGC TCCCTCGTGC GCTCTCCTGT 
     5941 TCCGACCCTG CCGCTTACCG GATACCTGTC CGCCTTTCTC CCTTCGGGAA GCGTGGCGCT 
     6001 TTCTCATAGC TCACGCTGTA GGTATCTCAG TTCGGTGTAG GTCGTTCGCT CCAAGCTGGG 
     6061 CTGTGTGCAC GAACCCCCCG TTCAGCCCGA CCGCTGCGCC TTATCCGGTA ACTATCGTCT 
     6121 TGAGTCCAAC CCGGTAAGAC ACGACTTATC GCCACTGGCA GCAGCCACTG GTAACAGGAT 
     6181 TAGCAGAGCG AGGTATGTAG GCGGTGCTAC AGAGTTCTTG AAGTGGTGGC CTAACTACGG 
     6241 CTACACTAGA AGAACAGTAT TTGGTATCTG CGCTCTGCTG AAGCCAGTTA CCTTCGGAAA 
     6301 AAGAGTTGGT AGCTCTTGAT CCGGCAAACA AACCACCGCT GGTAGCGGTG GTTTTTTTGT 
     6361 TTGCAAGCAG CAGATTACGC GCAGAAAAAA AGGATCTCAA GAAGATCCTT TGATCTTTTC 
     6421 TACGGGGTCT GACGCTCAGT GGAACGAAAA CTCACGTTAA GGGATTTTGG TCATGAGATT 
     6481 ATCAAAAAGG ATCTTCACCT AGATCCTTTT AAATTAAAAA TGAAGTTTTA AATCAATCTA 
     6541 AAGTATATAT GAGTAACCTG AGGCTATGGC AGGGCCTGCC GCCCCGACGT TGGCTGCGAG 
     6601 CCCTGGGCCT TCACCCGAAC TTGGGGGGTG GGGTGGGGAA AAGGAAGAAA CGCGGGCGTA 
     6661 TTGGCCCCAA TGGGGTCTCG GTGGGGTATC GACAGAGTGC CAGCCCTGGG ACCGAACCCC 
     6721 GCGTTTATGA ACAAACGACC CAACACCGTG CGTTTTATTC TGTCTTTTTA TTGCCGTCAT 
     6781 AGCGCGGGTT CCTTCCGGTA TTGTCTCCTT CCGTGTTTCA GTTAGCCTCC CCCTAGGGTG 
     6841 GGCGAAGAAC TCCAGCATGA GATCCCCGCG CTGGAGGATC ATCCAGCCGG CGTCCCGGAA 
     6901 AACGATTCCG AAGCCCAACC TTTCATAGAA GGCGGCGGTG GAATCGAAAT CTCGTGATGG 
     6961 CAGGTTGGGC GTCGCTTGGT CGGTCATTTC GAACCCCAGA GTCCCGCTCA GAAGAACTCG 
     7021 TCAAGAAGGC GATAGAAGGC GATGCGCTGC GAATCGGGAG CGGCGATACC GTAAAGCACG 
     7081 AGGAAGCGGT CAGCCCATTC GCCGCCAAGC TCTTCAGCAA TATCACGGGT AGCCAACGCT 
     7141 ATGTCCTGAT AGCGATCCGC CACACCCAGC CGGCCACAGT CGATGAATCC AGAAAAGCGG 
     7201 CCATTTTCCA CCATGATATT CGGCAAGCAG GCATCGCCAT GGGTCACGAC GAGATCCTCG 
     7261 CCGTCGGGCA TGCTCGCCTT GAGCCTGGCG AACAGTTCGG CTGGCGCGAG CCCCTGATGC 
     7321 TCTTCGTCCA GATCATCCTG ATCGACAAGA CCGGCTTCCA TCCGAGTACG TGCTCGCTCG 
     7381 ATGCGATGTT TCGCTTGGTG GTCGAATGGG CAGGTAGCCG GATCAAGCGT ATGCAGCCGC 
     7441 CGCATTGCAT CAGCCATGAT GGATACTTTC TCGGCAGGAG CAAGGTGAGA TGACAGGAGA 
     7501 TCCTGCCCCG GCACTTCGCC CAATAGCAGC CAGTCCCTTC CCGCTTCAGT GACAACGTCG 
     7561 AGCACAGCTG CGCAAGGAAC GCCCGTCGTG GCCAGCCACG ATAGCCGCGC TGCCTCGTCT 
     7621 TGCAGTTCAT TCAGGGCACC GGACAGGTCG GTCTTGACAA AAAGAACCGG GCGCCCCTGC 
     7681 GCTGACAGCC GGAACACGGC GGCATCAGAG CAGCCGATTG TCTGTTGTGC CCAGTCATAG 
     7741 CCGAATAGCC TCTCCACCCA AGCGGCCGGA GAACCTGCGT GCAATCCATC TTGTTCAATC 
     7801 ATGCGAAACG ATCCTCATCC TGTCTCTTGA TCGATCTTTG CAAAAGCCTA GGCCTCCAAA 
     7861 AAAGCCTCCT CACTACTTCT GGAATAGCTC AGAGGCCGAG GCGGCCTCGG CCTCTGCATA 
     7921 AATAAAAAAA ATTAGTCAGC CATGGGGCGG AGAATGGGCG GAACTGGGCG GAGTTAGGGG 
     7981 CGGGATGGGC GGAGTTAGGG GCGGGACTAT GGTTGCTGAC TAATTGAGAT GCATGCTTTG 
     8041 CATACTTCTG CCTGCTGGGG AGCCTGGGGA CTTTCCACAC CTGGTTGCTG ACTAATTGAG 
     8101 ATGCATGCTT TGCATACTTC TGCCTGCTGG GGAGCCTGGG GACTTTCCAC ACCCTAACTG 
     8161 ACACACATTC CACAGCTGGT TCTTTCCGCC TCAGGACTCT TCCTTTTTCA ATATTATTGA 
     8221 AGCATTTATC AGGGTTATTG TCTCATGAGC GGATACATAT TTGAATGTAT TTAGAAAAAT 
     8281 AAACAAATAG GGGTTCCGCG CACATTTCCC CGAAAAGTGC CACCTGACGC GCCCTGTAGC 
     8341 GGCGCATTAA GCGCGGCGGG TGTGGTGGTT ACGCGCAGCG TGACCGCTAC ACTTGCCAGC 
     8401 GCCCTAGCGC CCGCTCCTTT CGCTTTCTTC CCTTCCTTTC TCGCCACGTT CGCCGGCTTT 
     8461 CCCCGTCAAG CTCTAAATCG GGGGCTCCCT TTAGGGTTCC GATTTAGTGC TTTACGGCAC 
     8521 CTCGACCCCA AAAAACTTGA TTAGGGTGAT GGTTCACGTA GTGGGCCATC GCCCTGATAG 
     8581 ACGGTTTTTC GCCCTTTGAC GTTGGAGTCC ACGTTCTTTA ATAGTGGACT CTTGTTCCAA 
     8641 ACTGGAACAA CACTCAACCC TATCTCGGTC TATTCTTTTG ATTTATAAGG GATTTTGCCG 
     8701 ATTTCGGCCT ATTGGTTAAA AAATGAGCTG ATTTAACAAA AATTTAACGC GAATTTT
//