LOCUS       RC213566L1                9489 bp    DNA     circular  SYN 
DEFINITION  TNK2 (Myc-DDK-tagged)-Human tyrosine kinase, non-receptor, 2 (TNK2), transcript variant 1
ACCESSION   NM_005781
VERSION     null
SOURCE      synthetic construct 
FEATURES    Location/Qualifiers 
     misc_feature    7780..7960 
                     /label="3'LTR"  
     misc_feature    833..1013 
                     /label="5'LTR"  
     misc_feature    8688..9347 
                     /label="CAT(Cam-r)"  
     misc_feature    2620..3234 
                     /label="CMV promoter"  
     misc_feature    8119..8687 
                     /label="ColE1"  
     misc_feature    6655..6675 
                     /label="LR50 primer"  
     misc_feature    6452..6523 
                     /label="Myc-DDK"  
     misc_feature    3252..3271 
                     /label="T7 promoter"  
     misc_feature    3221..3237 
                     /label="V2 primer"  
     misc_feature    6664..7255 
                     /label="WPRE"  
     misc_feature    3320..3328 
                     /label="Restriction Enzyme"  
     misc_feature    6434..6440 
                     /label="Restriction Enzyme"  
     misc_feature    3320..6434 
                     /label="TNK2"  
ORIGIN                      
        1 GTCGACGGAT CGGGAGATCT CCCGATCCCC TATGGTGCAC TCTCAGTACA ATCTGCTCTG 
       61 ATGCCGCATA GTTAAGCCAG TATCTGCTCC CTGCTTGTGT GTTGGAGGTC GCTGAGTAGT 
      121 GCGCGAGCAA AATTTAAGCT ACAACAAGGC AAGGCTTGAC CGACAATTGC ATGAAGAATC 
      181 TGCTTAGGGT TAGGCGTTTT GCGCTGCTTC GCGATGTACG GGCCAGATAT CGCGTTGACA 
      241 TTGATTATTG ACTAGTTATT AATAGTAATC AATTACGGGG TCATTAGTTC ATAGCCCATA 
      301 TATGGAGTTC CGCGTTACAT AACTTACGGT AAATGGCCCG CCTGGCTGAC CGCCCAACGA 
      361 CCCCCGCCCA TTGACGTCAA TAATGACGTA TGTTCCCATA GTAACGCCAA TAGGGACTTT 
      421 CCATTGACGT CAATGGGTGG AGTATTTACG GTAAACTGCC CACTTGGCAG TACATCAAGT 
      481 GTATCATATG CCAAGTACGC CCCCTATTGA CGTCAATGAC GGTAAATGGC CCGCCTGGCA 
      541 TTATGCCCAG TACATGACCT TATGGGACTT TCCTACTTGG CAGTACATCT ACGTATTAGT 
      601 CATCGCTATT ACCATGGTGA TGCGGTTTTG GCAGTACATC AATGGGCGTG GATAGCGGTT 
      661 TGACTCACGG GGATTTCCAA GTCTCCACCC CATTGACGTC AATGGGAGTT TGTTTTGGCA 
      721 CCAAAATCAA CGGGACTTTC CAAAATGTCG TAACAACTCC GCCCCATTGA CGCAAATGGG 
      781 CGGTAGGCGT GTACGGTGGG AGGTCTATAT AAGCAGCGCG TTTTGCCTGT ACTGGGTCTC 
      841 TCTGGTTAGA CCAGATCTGA GCCTGGGAGC TCTCTGGCTA ACTAGGGAAC CCACTGCTTA 
      901 AGCCTCAATA AAGCTTGCCT TGAGTGCTTC AAGTAGTGTG TGCCCGTCTG TTGTGTGACT 
      961 CTGGTAACTA GAGATCCCTC AGACCCTTTT AGTCAGTGTG GAAAATCTCT AGCAGTGGCG 
     1021 CCCGAACAGG GACTTGAAAG CGAAAGGGAA ACCAGAGGAG CTCTCTCGAC GCAGGACTCG 
     1081 GCTTGCTGAA GCGCGCACGG CAAGAGGCGA GGGGCGGCGA CTGGTGAGTA CGCCAAAAAT 
     1141 TTTGACTAGC GGAGGCTAGA AGGAGAGAGA TGGGTGCGAG AGCGTCAGTA TTAAGCGGGG 
     1201 GAGAATTAGA TCGCGATGGG AAAAAATTCG GTTAAGGCCA GGGGGAAAGA AAAAATATAA 
     1261 ATTAAAACAT ATAGTATGGG CAAGCAGGGA GCTAGAACGA TTCGCAGTTA ATCCTGGCCT 
     1321 GTTAGAAACA TCAGAAGGCT GTAGACAAAT ACTGGGACAG CTACAACCAT CCCTTCAGAC 
     1381 AGGATCAGAA GAACTTAGAT CATTATATAA TACAGTAGCA ACCCTCTATT GTGTGCATCA 
     1441 AAGGATAGAG ATAAAAGACA CCAAGGAAGC TTTAGACAAG ATAGAGGAAG AGCAAAACAA 
     1501 AAGTAAGACC ACCGCACAGC AAGCGGCCGG CCGCTGATCT TCAGACCTGG AGGAGGAGAT 
     1561 ATGAGGGACA ATTGGAGAAG TGAATTATAT AAATATAAAG TAGTAAAAAT TGAACCATTA 
     1621 GGAGTAGCAC CCACCAAGGC AAAGAGAAGA GTGGTGCAGA GAGAAAAAAG AGCAGTGGGA 
     1681 ATAGGAGCTT TGTTCCTTGG GTTCTTGGGA GCAGCAGGAA GCACTATGGG CGCAGCGTCA 
     1741 ATGACGCTGA CGGTACAGGC CAGACAATTA TTGTCTGGTA TAGTGCAGCA GCAGAACAAT 
     1801 TTGCTGAGGG CTATTGAGGC GCAACAGCAT CTGTTGCAAC TCACAGTCTG GGGCATCAAG 
     1861 CAGCTCCAGG CAAGAATCCT GGCTGTGGAA AGATACCTAA AGGATCAACA GCTCCTGGGG 
     1921 ATTTGGGGTT GCTCTGGAAA ACTCATTTGC ACCACTGCTG TGCCTTGGAA TGCTAGTTGG 
     1981 AGTAATAAAT CTCTGGAACA GATTTGGAAT CACACGACCT GGATGGAGTG GGACAGAGAA 
     2041 ATTAACAATT ACACAAGCTT AATACACTCC TTAATTGAAG AATCGCAAAA CCAGCAAGAA 
     2101 AAGAATGAAC AAGAATTATT GGAATTAGAT AAATGGGCAA GTTTGTGGAA TTGGTTTAAC 
     2161 ATAACAAATT GGCTGTGGTA TATAAAATTA TTCATAATGA TAGTAGGAGG CTTGGTAGGT 
     2221 TTAAGAATAG TTTTTGCTGT ACTTTCTATA GTGAATAGAG TTAGGCAGGG ATATTCACCA 
     2281 TTATCGTTTC AGACCCACCT CCCAACCCCG AGGGGACCCG ACAGGCCCGA AGGAATAGAA 
     2341 GAAGAAGGTG GAGAGAGAGA CAGAGACAGA TCCATTCGAT TAGTGAACGG ATCGGCACTG 
     2401 CGTGCGCCAA TTCTGCAGAC AAATGGCAGT ATTCATCCAC AATTTTAAAA GAAAAGGGGG 
     2461 GATTGGGGGG TACAGTGCAG GGGAAAGAAT AGTAGACATA ATAGCAACAG ACATACAAAC 
     2521 TAAAGAATTA CAAAAACAAA TTACAAAAAT TCAAAATTTT CGGGTTTATT ACAGGGACAG 
     2581 CAGAGATCCA GTTTGGTTAG TACCGGGCCC GCTCTAGACA TGTCCAATAT GACCGCCATG 
     2641 TTGACATTGA TTATTGACTA GTTATTAATA GTAATCAATT ACGGGGTCAT TAGTTCATAG 
     2701 CCCATATATG GAGTTCCGCG TTACATAACT TACGGTAAAT GGCCCGCCTG GCTGACCGCC 
     2761 CAACGACCCC CGCCCATTGA CGTCAATAAT GACGTATGTT CCCATAGTAA CGCCAATAGG 
     2821 GACTTTCCAT TGACGTCAAT GGGTGGAGTA TTTACGGTAA ACTGCCCACT TGGCAGTACA 
     2881 TCAAGTGTAT CATATGCCAA GTCCGCCCCC TATTGACGTC AATGACGGTA AATGGCCCGC 
     2941 CTGGCATTAT GCCCAGTACA TGACCTTACG GGACTTTCCT ACTTGGCAGT ACATCTACGT 
     3001 ATTAGTCATC GCTATTACCA TGGTGATGCG GTTTTGGCAG TACACCAATG GGCGTGGATA 
     3061 GCGGTTTGAC TCACGGGGAT TTCCAAGTCT CCACCCCATT GACGTCAATG GGAGTTTGTT 
     3121 TTGGCACCAA AATCAACGGG ACTTTCCAAA ATGTCGTAAT AACCCCGCCC CGTTGACGCA 
     3181 AATGGGCGGT AGGCGTGTAC GGTGGGAGGT CTATATAAGC AGAGCTCGTT TAGTGAACCG 
     3241 TCAGAATTTT GTAATACGAC TCACTATAGG GCGGCCGGGA ATTCGTCGAC TGGATCCGGT 
     3301 ACCGAGGAGA TCTGCCGCCG CGATCGCCAT GCAGCCAGAG GAGGGCACAG GCTGGCTGCT 
     3361 GGAGCTGCTG TCCGAGGTGC AGCTGCAACA GTACTTCCTG CGGCTCCGAG ATGACCTCAA 
     3421 CGTCACCCGC CTGTCCCACT TTGAGTACGT CAAGAATGAG GACCTGGAGA AGATCGGCAT 
     3481 GGGTCGGCCT GGCCAGCGGC GGCTGTGGGA GGCTGTGAAG AGGAGGAAGG CCTTGTGCAA 
     3541 ACGCAAGTCG TGGATGAGTA AGGTGTTCAG TGGAAAGCGA CTGGAGGCTG AGTTCCCACC 
     3601 TCATCACTCT CAGAGCACCT TCCGGAAGAC CTCGCCCGCC CCTGGGGGCC CAGCAGGGGA 
     3661 GGGGCCCCTG CAGAGCCTCA CCTGCCTCAT TGGGGAGAAG GACCTGCGCC TCCTGGAGAA 
     3721 GCTGGGTGAT GGTTCCTTTG GCGTGGTGCG CAGGGGCGAG TGGGACGCGC CCTCAGGGAA 
     3781 GACGGTGAGT GTGGCTGTGA AGTGCCTGAA GCCCGATGTC CTGAGCCAGC CAGAAGCCAT 
     3841 GGACGACTTC ATCCGGGAGG TCAATGCCAT GCACTCGCTC GACCACCGAA ACCTCATCCG 
     3901 CCTCTACGGG GTGGTGCTCA CGCCGCCCAT GAAGATGGTG ACAGAGCTGG CACCTCTGGG 
     3961 ATCGTTGTTG GACCGGCTAC GTAAGCACCA GGGCCACTTC CTCCTGGGGA CTCTGAGCCG 
     4021 CTACGCTGTG CAGGTGGCTG AGGGCATGGG CTACCTGGAG TCCAAGCGCT TTATTCACCG 
     4081 TGACCTGGCT GCCCGCAATC TGCTGTTGGC TACCCGCGAC CTGGTCAAGA TCGGGGACTT 
     4141 TGGGCTGATG CGAGCACTAC CTCAGAATGA CGACCATTAC GTCATGCAGG AACATCGCAA 
     4201 GGTGCCCTTC GCCTGGTGTG CCCCCGAGAG CCTGAAGACA CGCACCTTCT CCCATGCCAG 
     4261 CGACACCTGG ATGTTCGGGG TGACACTGTG GGAAATGTTC ACCTACGGCC AGGAGCCCTG 
     4321 GATCGGCCTC AACGGCAGTC AGATCCTGCA TAAGATCGAC AAGGAGGGGG AGCGGCTGCC 
     4381 CCGGCCCGAG GACTGTCCCC AGGACATCTA CAACGTCATG GTCCAGTGCT GGGCTCACAA 
     4441 GCCAGAGGAC AGACCCACGT TTGTGGCCCT GCGGGACTTC CTGCTGGAGG CCCAGCCCAC 
     4501 AGACATGCGG GCCCTTCAGG ACTTTGAGGA ACCGGACAAG CTGCACATCC AGATGAATGA 
     4561 TGTCATCACC GTCATCGAGG GAAGGGCCGA GAACTACTGG TGGCGTGGCC AGAACACACG 
     4621 GACGCTGTGT GTGGGGCCCT TCCCTCGCAA CGTGGTGACC TCCGTGGCCG GCCTGTCGGC 
     4681 CCAGGACATC AGCCAGCCCC TGCAGAACAG CTTCATCCAC ACAGGGCATG GCGACAGTGA 
     4741 CCCCCGCCAC TGCTGGGGCT TCCCGGACAG GATTGACGAA CTGTATCTGG GAAACCCCAT 
     4801 GGACCCCCCC GACCTCCTGA GCGTGGAACT GAGCACCTCC CGGCCCCCCC AGCATCTAGG 
     4861 AGGGGTGAAA AAACCAACCT ATGACCCTGT GAGCGAGGAC CAAGACCCCT TGTCCAGCGA 
     4921 CTTCAAGAGG CTGGGCCTGC GGAAGCCAGG CCTGCCCCGA GGGCTGTGGC TGGCGAAGCC 
     4981 CTCGGCGCGG GTGCCGGGCA CCAAGGCCAG CCGAGGCAGC GGGGCTGAGG TCACGCTCAT 
     5041 CGACTTCGGT GAGGAGCCCG TGGTCCCGGC CCTACGGCCC TGCGCGCCCT CCCTGGCGCA 
     5101 GCTGGCCATG GACGCCTGCT CCCTGCTGGA CGAGACCCCG CCTCAGAGCC CCACGCGGGC 
     5161 ACTGCCCCGG CCCCTGCACC CCACGCCTGT GGTGGACTGG GACGCACGCC CGCTGCCCCC 
     5221 CCCGCCCGCC TATGACGACG TGGCCCAGGA TGAGGATGAC TTTGAGATCT GCTCCATCAA 
     5281 CAGCACCCTC GTGGGCGCGG GGGTCCCTGC CGGGCCCAGC CAGGGCCAGA CCAACTACGC 
     5341 CTTTGTGCCT GAGCAGGCGC GGCCGCCCCC TCCCCTGGAG GACAACCTGT TCCTCCCGCC 
     5401 CCAGGGTGGG GGCAAGCCGC CCAGCTCCGC ACAGACCGCA GAGATCTTCC AGGCGCTACA 
     5461 GCAGGAGTGC ATGAGGCAAC TGCAGGCTCC GGCCGGCTCC CCGGCCCCCT CTCCCAGCCC 
     5521 GGGGGGTGAC GACAAGCCCC AGGTGCCTCC TCGGGTACCC ATCCCCCCTC GGCCCACGCG 
     5581 CCCACACGTC CAGCTGTCTC CAGCCCCCCC GGGCGAGGAG GAGACCAGCC AGTGGCCTGG 
     5641 ACCTGCTTCC CCTCCCCGGG TGCCTCCGCG GGAGCCCCTG TCCCCTCAAG GCTCGAGGAC 
     5701 ACCCAGCCCC CTGGTACCAC CTGGCAGCTC CCCGCTGCCA CCCCGGCTCT CAAGCTCACC 
     5761 TGGGAAGACC ATGCCCACCA CCCAGAGCTT TGCCTCAGAC CCCAAGTACG CCACCCCCCA 
     5821 GGTGATCCAG GCCCCTGGCC CGCGGGCTGG TCCCTGCATC CTGCCCATCG TCCGGGATGG 
     5881 CAAGAAGGTC AGCAGCACCC ACTATTACTT GCTGCCCGAG CGACCATCCT ACCTGGAGCG 
     5941 CTACCAGCGC TTCCTGCGTG AGGCCCAGAG CCCCGAGGAG CCTACCCCCC TGCCTGTGCC 
     6001 TCTGCTGCTG CCCCCACCCA GCACCCCAGC CCCCGCCGCC CCCACGGCCA CCGTGCGGCC 
     6061 GATGCCCCAG GCTGCCTTGG ACCCCAAGGC CAACTTCTCC ACCAACAACA GCAACCCAGG 
     6121 GGCCCGGCCA CCACCCCCGA GGGCCACTGC TCGGCTGCCA CAGAGGGGCT GCCCTGGCGA 
     6181 TGGGCCAGAG GCGGGCCGGC CAGCAGACAA GATCCAGATG GCCATGGTGC ATGGGGTGAC 
     6241 CACAGAGGAG TGCCAGGCGG CCCTGCAGTG CCACGGCTGG AGCGTGCAGA GGGCTGCCCA 
     6301 GTATCTGAAG GTGGAGCAGC TCTTCGGGCT GGGTCTGCGG CCCAGAGGGG AGTGCCACAA 
     6361 AGTGCTGGAG ATGTTCGACT GGAACCTGGA GCAGGCCGGC TGCCACCTTC TGGGCTCCTG 
     6421 GGGCCCTGCC CACCACAAGC GCACGCGTAC GCGGCCGCTC GAGCAGAAAC TCATCTCAGA 
     6481 AGAGGATCTG GCAGCAAATG ATATCCTGGA TTACAAGGAT GACGACGATA AGGTTTAAAC 
     6541 GGCCGGCCGC GGTCTGTACA AGTAGGATTC GTCGAGGGAC CTAATAACTT CGTATAGCAT 
     6601 ACATTATACG AAGTTATACA TGTTTAAGGG TTCCGGTTCC ACTAGGTACA ATTCGATATC 
     6661 AAGCTTATCG ATAATCAACC TCTGGATTAC AAAATTTGTG AAAGATTGAC TGGTATTCTT 
     6721 AACTATGTTG CTCCTTTTAC GCTATGTGGA TACGCTGCTT TAATGCCTTT GTATCATGCT 
     6781 ATTGCTTCCC GTATGGCTTT CATTTTCTCC TCCTTGTATA AATCCTGGTT GCTGTCTCTT 
     6841 TATGAGGAGT TGTGGCCCGT TGTCAGGCAA CGTGGCGTGG TGTGCACTGT GTTTGCTGAC 
     6901 GCAACCCCCA CTGGTTGGGG CATTGCCACC ACCTGTCAGC TCCTTTCCGG GACTTTCGCT 
     6961 TTCCCCCTCC CTATTGCCAC GGCGGAACTC ATCGCCGCCT GCCTTGCCCG CTGCTGGACA 
     7021 GGGGCTCGGC TGTTGGGCAC TGACAATTCC GTGGTGTTGT CGGGGAAATC ATCGTCCTTT 
     7081 CCTTGGCTGC TCGCCTGTGT TGCCACCTGG ATTCTGCGCG GGACGTCCTT CTGCTACGTC 
     7141 CCTTCGGCCC TCAATCCAGC GGACCTTCCT TCCCGCGGCC TGCTGCCGGC TCTGCGGCCT 
     7201 CTTCCGCGTC TTCGCCTTCG CCCTCAGACG AGTCGGATCT CCCTTTGGGC CGCCTCCCCG 
     7261 CATCGATACC GTCGACCTCG ATCGAGACCT AGAAAAACAT GGAGCAATCA CAAGTAGCAA 
     7321 TACAGCAGCT ACCAATGCTG ATTGTGCCTG GCTAGAAGCA CAAGAGGAGG AGGAGGTGGG 
     7381 TTTTCCAGTC ACACCTCAGG TACCTTTAAG ACCAATGACT TACAAGGCAG CTGTAGATCT 
     7441 TAGCCACTTT TTAAAAGAAA AGGGGGGACT GGAAGGGCTA ATTCACTCCC AACGAAGACA 
     7501 AGATATCCTT GATCTGTGGA TCTACCACAC ACAAGGCTAC TTCCCTGATT GGCAGAACTA 
     7561 CACACCAGGG CCAGGGATCA GATATCCACT GACCTTTGGA TGGTGCTACA AGCTAGTACC 
     7621 AGTTGAGCAA GAGAAGGTAG AAGAAGCCAA TGAAGGAGAG AACACCCGCT TGTTACACCC 
     7681 TGTGAGCCTG CATGGGATGG ATGACCCGGA GAGAGAAGTA TTAGAGTGGA GGTTTGACAG 
     7741 CCGCCTAGCA TTTCATCACA TGGCCCGAGA GCTGCATCCG GACTGTACTG GGTCTCTCTG 
     7801 GTTAGACCAG ATCTGAGCCT GGGAGCTCTC TGGCTAACTA GGGAACCCAC TGCTTAAGCC 
     7861 TCAATAAAGC TTGCCTTGAG TGCTTCAAGT AGTGTGTGCC CGTCTGTTGT GTGACTCTGG 
     7921 TAACTAGAGA TCCCTCAGAC CCTTTTAGTC AGTGTGGAAA ATCTCTAGCA GCATGTGAGC 
     7981 AAAAGGCCAG CAAAAGGCCA GGAACCGTAA AAAGGCCGCG TTGCTGGCGT TTTTCCATAG 
     8041 GCTCCGCCCC CCTGACGAGC ATCACAAAAA TCGACGCTCA AGTCAGAGGT GGCGAAACCC 
     8101 GACAGGACTA TAAAGATACC AGGCGTTTCC CCCTGGAAGC TCCCTCGTGC GCTCTCCTGT 
     8161 TCCGACCCTG CCGCTTACCG GATACCTGTC CGCCTTTCTC CCTTCGGGAA GCGTGGCGCT 
     8221 TTCTCATAGC TCACGCTGTA GGTATCTCAG TTCGGTGTAG GTCGTTCGCT CCAAGCTGGG 
     8281 CTGTGTGCAC GAACCCCCCG TTCAGCCCGA CCGCTGCGCC TTATCCGGTA ACTATCGTCT 
     8341 TGAGTCCAAC CCGGTAAGAC ACGACTTATC GCCACTGGCA GCAGCCACTG GTAACAGGAT 
     8401 TAGCAGAGCG AGGTATGTAG GCGGTGCTAC AGAGTTCTTG AAGTGGTGGC CTAACTACGG 
     8461 CTACACTAGA AGAACAGTAT TTGGTATCTG CGCTCTGCTG AAGCCAGTTA CCTTCGGAAA 
     8521 AAGAGTTGGT AGCTCTTGAT CCGGCAAACA AACCACCGCT GGTAGCGGTG GTTTTTTTGT 
     8581 TTGCAAGCAG CAGATTACGC GCAGAAAAAA AGGATCTCAA GAAGATCCTT TGATCTTTTC 
     8641 TACGGGGTCT GACGCTCAGT GGAACGAAAA CTCACGTTAA GGGATTTTGG TCATGATTAC 
     8701 GCCCCGCCCT GCCACTCATC GCAGTACTGT TGTAATTCAT TAAGCATTCT GCCGACATGG 
     8761 AAGCCATCAC AAACGGCATG ATGAACCTGA ATCGCCAGCG GCATCAGCAC CTTGTCGCCT 
     8821 TGCGTATAAT ATTTGCCCAT GGTGAAAACG GGGGCGAAGA AGTTGTCCAT ATTGGCCACG 
     8881 TTTAAATCAA AACTGGTGAA ACTCACCCAG GGATTGGCTG AGACGAAAAA CATATTCTCA 
     8941 ATAAACCCTT TAGGGAAATA GGCCAGGTTT TCACCGTAAC ACGCCACATC TTGCGAATAT 
     9001 ATGTGTAGAA ACTGCCGGAA ATCGTCGTGG TATTCACTCC AGAGCGATGA AAACGTTTCA 
     9061 GTTTGCTCAT GGAAAACGGT GTAACAAGGG TGAACACTAT CCCATATCAC CAGCTCACCG 
     9121 TCTTTCATTG CCATACGGAA CTCCGGATGA GCATTCATCA GGCGGGCAAG AATGTGAATA 
     9181 AAGGCCGGAT AAAACTTGTG CTTATTTTTC TTTACGGTCT TTAAAAAGGC CGTAATATCC 
     9241 AGCTGAACGG TCTGGTTATA GGTACATTGA GCAACTGACT GAAATGCCTC AAAATGTTCT 
     9301 TTACGATGCC ATTGGGATAT ATCAACGGTG GTATATCCAG TGATTTTTTT CTCCATACTC 
     9361 TTCCTTTTTC AATATTATTG AAGCATTTAT CAGGGTTATT GTCTCATGAG CGGATACATA 
     9421 TTTGAATGTA TTTAGAAAAA TAAACAAATA GGGGTCCCGC GCACATTTCC CCGAAAAGTG 
     9481 CCACCTGAC
//