LOCUS       RC213564                8625 bp    DNA     circular  SYN 
DEFINITION  APAF1 (Myc-DDK-tagged)-Human apoptotic peptidase activating factor 1 (APAF1), transcript variant 3
ACCESSION   NM_181861
VERSION     NM_181861.1
SOURCE      synthetic construct 
FEATURES    Location/Qualifiers 
     misc_feature    201..924 
                     /label="CMV promoter"  
     misc_feature    839..856 
                     /label="Restriction Enzyme"  
     misc_feature    953..971 
                     /label="T7 promoter"  
     misc_feature    4782..4873 
                     /label="Myc-DDK"  
     misc_feature    4903..5488 
                     /label="PolyA signal"  
     misc_feature    4960..4979 
                     /label="Restriction Enzyme"  
     misc_feature    5764..6436 
                     /label="ColE1"  
     misc_feature    6866..7660 
                     /label="Kan/Neo"  
     misc_feature    7695..7997 
                     /label="SV40 ori"  
     misc_feature    8083..8560 
                     /label="f1 ori"  
     misc_feature    1020..1028 
                     /label="Restriction Enzyme"  
     misc_feature    4764..4770 
                     /label="Restriction Enzyme"  
     misc_feature    1020..4764 
                     /label="APAF1"  
ORIGIN                      
        1 AACAAAATAT TAACGCTTAC AATTTCCATT CGCCATTCAG GCTGCGCAAC TGTTGGGAAG 
       61 GGCGATCGGT GCGGGCCTCT TCGCTATTAC GCCAGCTGGC GAAAGGGGGA TGTGCTGCAA 
      121 GGCGATTAAG TTGGGTAACG CCAGGGTTTT CCCAGTCACG ACGTTGTAAA ACGACGGCCA 
      181 GTGCCAAGCT GATCTATACA TTGAATCAAT ATTGGCAATT AGCCATATTA GTCATTGGTT 
      241 ATATAGCATA AATCAATATT GGCTATTGGC CATTGCATAC GTTGTATCTA TATCATAATA 
      301 TGTACATTTA TATTGGCTCA TGTCCAATAT GACCGCCATG TTGACATTGA TTATTGACTA 
      361 GTTATTAATA GTAATCAATT ACGGGGTCAT TAGTTCATAG CCCATATATG GAGTTCCGCG 
      421 TTACATAACT TACGGTAAAT GGCCCGCCTG GCTGACCGCC CAACGACCCC CGCCCATTGA 
      481 CGTCAATAAT GACGTATGTT CCCATAGTAA CGCCAATAGG GACTTTCCAT TGACGTCAAT 
      541 GGGTGGAGTA TTTACGGTAA ACTGCCCACT TGGCAGTACA TCAAGTGTAT CATATGCCAA 
      601 GTCCGCCCCC TATTGACGTC AATGACGGTA AATGGCCCGC CTGGCATTAT GCCCAGTACA 
      661 TGACCTTACG GGACTTTCCT ACTTGGCAGT ACATCTACGT ATTAGTCATC GCTATTACCA 
      721 TGGTGATGCG GTTTTGGCAG TACACCAATG GGCGTGGATA GCGGTTTGAC TCACGGGGAT 
      781 TTCCAAGTCT CCACCCCATT GACGTCAATG GGAGTTTGTT TTGGCACCAA AATCAACGGG 
      841 ACTTTCCAAA ATGTCGTAAT AACCCCGCCC CGTTGACGCA AATGGGCGGT AGGCGTGTAC 
      901 GGTGGGAGGT CTATATAAGC AGAGCTCGTT TAGTGAACCG TCAGAATTTT GTAATACGAC 
      961 TCACTATAGG GCGGCCGGGA ATTCGTCGAC TGGATCCGGT ACCGAGGAGA TCTGCCGCCG 
     1021 CGATCGCCAT GGATGCAAAA GCTCGAAATT GTTTGCTTCA ACATAGAGAA GCTCTGGAAA 
     1081 AGGACATCAA GACATCCTAC ATCATGGATC ACATGATTAG TGATGGATTT TTAACAATAT 
     1141 CAGAAGAGGA AAAAGTAAGA AATGAGCCCA CTCAACAGCA AAGAGCAGCT ATGCTGATTA 
     1201 AAATGATACT TAAAAAAGAT AATGATTCCT ACGTATCATT CTACAATGCT CTACTACATG 
     1261 AAGGATATAA AGATCTTGCT GCCCTTCTCC ATGATGGCAT TCCTGTTGTC TCTTCTTCCA 
     1321 GTGGTAAAGA TTCAGTTAGT GGAATAACTT CGTATGTAAG GACAGTCCTG TGTGAAGGTG 
     1381 GAGTACCACA GAGGCCAGTT GTTTTTGTCA CAAGGAAGAA GCTGGTGAAT GCAATTCAGC 
     1441 AGAAGCTCTC CAAATTGAAA GGTGAACCAG GATGGGTCAC CATACATGGA ATGGCAGGCT 
     1501 GTGGGAAGTC TGTATTAGCT GCAGAAGCTG TTAGAGATCA TTCCCTTTTA GAAGGTTGTT 
     1561 TCCCAGGGGG AGTGCATTGG GTTTCAGTTG GGAAACAAGA CAAATCTGGG CTTCTGATGA 
     1621 AACTGCAGAA TCTTTGCACA CGGTTGGATC AGGATGAGAG TTTTTCCCAG AGGCTTCCAC 
     1681 TTAATATTGA AGAGGCTAAA GACCGTCTCC GCATTCTGAT GCTTCGCAAA CACCCAAGGT 
     1741 CTCTCTTGAT CTTGGATGAT GTTTGGGACT CTTGGGTGTT GAAAGCTTTT GACAGTCAGT 
     1801 GTCAGATTCT TCTTACAACC AGAGACAAGA GTGTTACAGA TTCAGTAATG GGTCCTAAAT 
     1861 ATGTAGTCCC TGTGGAGAGT TCCTTAGGAA AGGAAAAAGG ACTTGAAATT TTATCCCTTT 
     1921 TTGTTAATAT GAAGAAGGCA GATTTGCCAG AACAAGCTCA TAGTATTATA AAAGAATGTA 
     1981 AAGGCTCTCC CCTTGTAGTA TCTTTAATTG GTGCACTTTT ACGTGATTTT CCCAATCGCT 
     2041 GGGAGTACTA CCTCAAACAG CTTCAGAATA AGCAGTTTAA GAGAATAAGG AAATCTTCGT 
     2101 CTTATGATTA TGAGGCTCTA GATGAAGCCA TGTCTATAAG TGTTGAAATG CTCAGAGAAG 
     2161 ACATCAAAGA TTATTACACA GATCTTTCCA TCCTTCAGAA GGACGTTAAG GTGCCTACAA 
     2221 AGGTGTTATG TATTCTCTGG GACATGGAAA CTGAAGAAGT TGAAGACATA CTGCAGGAGT 
     2281 TTGTAAATAA GTCTCTTTTA TTCTGTGATC GGAATGGAAA GTCGTTTCGT TATTATTTAC 
     2341 ATGATCTTCA AGTAGATTTT CTTACAGAGA AGAATTGCAG CCAGCTTCAG GATCTACATA 
     2401 AGAAGATAAT CACTCAGTTT CAGAGATATC ACCAGCCGCA TACTCTTTCA CCAGATCAGG 
     2461 AAGACTGTAT GTATTGGTAC AACTTTCTGG CCTATCACAT GGCCAGTGCC AAGATGCACA 
     2521 AGGAACTTTG TGCTTTAATG TTTTCCCTGG ATTGGATTAA AGCAAAAACA GAACTTGTAG 
     2581 GCCCTGCTCA TCTGATTCAT GAATTTGTGG AATACAGACA TATACTAGAT GAAAAGGATT 
     2641 GTGCAGTCAG TGAGAATTTT CAGGAGTTTT TATCTTTAAA TGGACACCTT CTTGGACGAC 
     2701 AGCCATTTCC TAATATTGTA CAACTGGGTC TCTGTGAGCC GGAAACTTCA GAAGTTTATC 
     2761 AGCAAGCTAA GCTGCAGGCC AAGCAGGAGG TCGATAATGG AATGCTTTAC CTGGAATGGA 
     2821 TAAACAAAAA AAACATCACG AATCTTTCCC GCTTAGTTGT CCGCCCCCAC ACAGATGCTG 
     2881 TTTACCATGC CTGCTTTTCT GAGGATGGTC AGAGAATAGC TTCTTGTGGA GCTGATAAAA 
     2941 CCTTACAGGT GTTCAAAGCT GAAACAGGAG AGAAACTTCT AGAAATCAAG GCTCATGAGG 
     3001 ATGAAGTGCT TTGTTGTGCA TTCTCTACAG ATGACAGATT TATAGCAACC TGCTCAGTGG 
     3061 ATAAAAAAGT GAAGATTTGG AATTCTATGA CTGGGGAACT AGTACACACC TATGATGAGC 
     3121 ACTCAGAGCA AGTCAATTGC TGCCATTTCA CCAACAGTAG TCATCATCTT CTCTTAGCCA 
     3181 CTGGGTCAAG TGACTGCTTC CTCAAACTTT GGGATTTGAA TCAAAAAGAA TGTCGAAATA 
     3241 CCATGTTTGG TCATACAAAT TCAGTCAATC ACTGCAGATT TTCACCAGAT GATAAGCTTT 
     3301 TGGCTAGTTG TTCAGCTGAT GGAACCTTAA AGCTTTGGGA TGCGACATCA GCAAATGAGA 
     3361 GGAAAAGCAT TAATGTGAAA CAGTTCTTCC TAAATTTGGA GGACCCTCAA GAGGATATGG 
     3421 AAGTGATAGT GAAGTGTTGT TCGTGGTCTG CTGATGGTGC AAGGATAATG GTGGCAGCAA 
     3481 AAAATAAAAT CTTTCTTTTT GACATTCATA CTAGTGGCCT ATTGGGAGAA ATCCACACGG 
     3541 GCCATCACAG CACCATCCAG TACTGTGACT TCTCCCCACA AAACCATTTG GCAGTGGTTG 
     3601 CTTTGTCCCA GTACTGTGTA GAGTTGTGGA ATACAGACTC ACGTTCAAAG GTGGCTGATT 
     3661 GCAGAGGACA TTTAAGTTGG GTTCATGGTG TGATGTTTTC TCCTGATGGA TCATCATTTT 
     3721 TGACATCTTC TGATGACCAG ACAATCAGGC TCTGGGAGAC AAAGAAAGTA TGTAAGAACT 
     3781 CTGCTGTAAT GTTAAAGCAA GAAGTAGATG TTGTGTTTCA AGAAAATGAA GTGATGGTCC 
     3841 TTGCAGTTGA CCATATAAGA CGTCTGCAAC TCATTAATGG AAGAACAGGT CAGATTGATT 
     3901 ATCTGACTGA AGCTCAAGTT AGCTGCTGTT GCTTAAGTCC ACATCTTCAG TACATTGCAT 
     3961 TTGGAGATGA AAATGGAGCC ATTGAGATTT TAGAACTTGT AAACAATAGA ATCTTCCAGT 
     4021 CCAGGTTTCA GCACAAGAAA ACTGTATGGC ACATCCAGTT CACAGCCGAT GAGAAGACTC 
     4081 TTATTTCAAG TTCTGATGAT GCTGAAATTC AGGTATGGAA TTGGCAATTG GACAAATGTA 
     4141 TCTTTCTACG AGGCCATCAG GAAACAGTGA AAGACTTTAG ACTCTTGAAA AATTCAAGAC 
     4201 TGCTTTCTTG GTCATTTGAT GGAACAGTGA AGGTATGGAA TATTATTACT GGAAATAAAG 
     4261 AAAAAGACTT TGTCTGTCAC CAGGGTACAG TACTTTCTTG TGACATTTCT CACGATGCTA 
     4321 CCAAGTTTTC ATCTACCTCT GCTGACAAGA CTGCAAAGAT CTGGAGTTTT GATCTCCTTT 
     4381 TGCCACTTCA TGAATTGAGG GGCCACAACG GCTGTGTGCG CTGCTCTGCC TTCTCTGTGG 
     4441 ACAGTACCCT GCTGGCAACG GGAGATGACA ATGGAGAAAT CAGGATATGG AATGTCTCAA 
     4501 ACGGTGAGCT TCTTCATTTG TGTGCTCCGC TTTCAGAAGA AGGAGCTGCT ACCCATGGAG 
     4561 GCTGGGTGAC TGACCTTTGC TTTTCTCCAG ATGGCAAAAT GCTTATCTCT GCTGGAGGAT 
     4621 ATATTAAGTG GTGGAACGTT GTCACTGGGG AATCCTCACA GACCTTCTAC ACAAATGGAA 
     4681 CCAATCTTAA GAAAATACAC GTGTCCCCTG ACTTCAAAAC ATATGTGACT GTGGATAATC 
     4741 TTGGTATTTT ATATATTTTA CAGACTTTAG AAACGCGTAC GCGGCCGCTC GAGCAGAAAC 
     4801 TCATCTCAGA AGAGGATCTG GCAGCAAATG ATATCCTGGA TTACAAGGAT GACGACGATA 
     4861 AGGTTTAAAC GGCCGGCCGC GGTCATAGCT GTTTCCTGAA CAGATCCCGG GTGGCATCCC 
     4921 TGTGACCCCT CCCCAGTGCC TCTCCTGGCC CTGGAAGTTG CCACTCCAGT GCCCACCAGC 
     4981 CTTGTCCTAA TAAAATTAAG TTGCATCATT TTGTCTGACT AGGTGTCCTT CTATAATATT 
     5041 ATGGGGTGGA GGGGGGTGGT ATGGAGCAAG GGGCAAGTTG GGAAGACAAC CTGTAGGGCC 
     5101 TGCGGGGTCT ATTGGGAACC AAGCTGGAGT GCAGTGGCAC AATCTTGGCT CACTGCAATC 
     5161 TCCGCCTCCT GGGTTCAAGC GATTCTCCTG CCTCAGCCTC CCGAGTTGTT GGGATTCCAG 
     5221 GCATGCATGA CCAGGCTCAG CTAATTTTTG TTTTTTTGGT AGAGACGGGG TTTCACCATA 
     5281 TTGGCCAGGC TGGTCTCCAA CTCCTAATCT CAGGTGATCT ACCCACCTTG GCCTCCCAAA 
     5341 TTGCTGGGAT TACAGGCGTG AACCACTGCT CCCTTCCCTG TCCTTCTGAT TTTAAAATAA 
     5401 CTATACCAGC AGGAGGACGT CCAGACACAG CATAGGCTAC CTGGCCATGC CCAACCGGTG 
     5461 GGACATTTGA GTTGCTTGCT TGGCACTGTC CTCTCATGCG TTGGGTCCAC TCAGTAGATG 
     5521 CCTGTTGAAT TGGGTACGCG GCCAGCGGCG AGCGGTATCA GCTCACTCAA AGGCGGTAAT 
     5581 ACGGTTATCC ACAGAATCAG GGGATAACGC AGGAAAGAAC ATGTGAGCAA AAGGCCAGCA 
     5641 AAAGGCCAGG AACCGTAAAA AGGCCGCGTT GCTGGCGTTT TTCCATAGGC TCCGCCCCCC 
     5701 TGACGAGCAT CACAAAAATC GACGCTCAAG TCAGAGGTGG CGAAACCCGA CAGGACTATA 
     5761 AAGATACCAG GCGTTTCCCC CTGGAAGCTC CCTCGTGCGC TCTCCTGTTC CGACCCTGCC 
     5821 GCTTACCGGA TACCTGTCCG CCTTTCTCCC TTCGGGAAGC GTGGCGCTTT CTCATAGCTC 
     5881 ACGCTGTAGG TATCTCAGTT CGGTGTAGGT CGTTCGCTCC AAGCTGGGCT GTGTGCACGA 
     5941 ACCCCCCGTT CAGCCCGACC GCTGCGCCTT ATCCGGTAAC TATCGTCTTG AGTCCAACCC 
     6001 GGTAAGACAC GACTTATCGC CACTGGCAGC AGCCACTGGT AACAGGATTA GCAGAGCGAG 
     6061 GTATGTAGGC GGTGCTACAG AGTTCTTGAA GTGGTGGCCT AACTACGGCT ACACTAGAAG 
     6121 AACAGTATTT GGTATCTGCG CTCTGCTGAA GCCAGTTACC TTCGGAAAAA GAGTTGGTAG 
     6181 CTCTTGATCC GGCAAACAAA CCACCGCTGG TAGCGGTGGT TTTTTTGTTT GCAAGCAGCA 
     6241 GATTACGCGC AGAAAAAAAG GATCTCAAGA AGATCCTTTG ATCTTTTCTA CGGGGTCTGA 
     6301 CGCTCAGTGG AACGAAAACT CACGTTAAGG GATTTTGGTC ATGAGATTAT CAAAAAGGAT 
     6361 CTTCACCTAG ATCCTTTTAA ATTAAAAATG AAGTTTTAAA TCAATCTAAA GTATATATGA 
     6421 GTAACCTGAG GCTATGGCAG GGCCTGCCGC CCCGACGTTG GCTGCGAGCC CTGGGCCTTC 
     6481 ACCCGAACTT GGGGGGTGGG GTGGGGAAAA GGAAGAAACG CGGGCGTATT GGCCCCAATG 
     6541 GGGTCTCGGT GGGGTATCGA CAGAGTGCCA GCCCTGGGAC CGAACCCCGC GTTTATGAAC 
     6601 AAACGACCCA ACACCGTGCG TTTTATTCTG TCTTTTTATT GCCGTCATAG CGCGGGTTCC 
     6661 TTCCGGTATT GTCTCCTTCC GTGTTTCAGT TAGCCTCCCC CTAGGGTGGG CGAAGAACTC 
     6721 CAGCATGAGA TCCCCGCGCT GGAGGATCAT CCAGCCGGCG TCCCGGAAAA CGATTCCGAA 
     6781 GCCCAACCTT TCATAGAAGG CGGCGGTGGA ATCGAAATCT CGTGATGGCA GGTTGGGCGT 
     6841 CGCTTGGTCG GTCATTTCGA ACCCCAGAGT CCCGCTCAGA AGAACTCGTC AAGAAGGCGA 
     6901 TAGAAGGCGA TGCGCTGCGA ATCGGGAGCG GCGATACCGT AAAGCACGAG GAAGCGGTCA 
     6961 GCCCATTCGC CGCCAAGCTC TTCAGCAATA TCACGGGTAG CCAACGCTAT GTCCTGATAG 
     7021 CGATCCGCCA CACCCAGCCG GCCACAGTCG ATGAATCCAG AAAAGCGGCC ATTTTCCACC 
     7081 ATGATATTCG GCAAGCAGGC ATCGCCATGG GTCACGACGA GATCCTCGCC GTCGGGCATG 
     7141 CTCGCCTTGA GCCTGGCGAA CAGTTCGGCT GGCGCGAGCC CCTGATGCTC TTCGTCCAGA 
     7201 TCATCCTGAT CGACAAGACC GGCTTCCATC CGAGTACGTG CTCGCTCGAT GCGATGTTTC 
     7261 GCTTGGTGGT CGAATGGGCA GGTAGCCGGA TCAAGCGTAT GCAGCCGCCG CATTGCATCA 
     7321 GCCATGATGG ATACTTTCTC GGCAGGAGCA AGGTGAGATG ACAGGAGATC CTGCCCCGGC 
     7381 ACTTCGCCCA ATAGCAGCCA GTCCCTTCCC GCTTCAGTGA CAACGTCGAG CACAGCTGCG 
     7441 CAAGGAACGC CCGTCGTGGC CAGCCACGAT AGCCGCGCTG CCTCGTCTTG CAGTTCATTC 
     7501 AGGGCACCGG ACAGGTCGGT CTTGACAAAA AGAACCGGGC GCCCCTGCGC TGACAGCCGG 
     7561 AACACGGCGG CATCAGAGCA GCCGATTGTC TGTTGTGCCC AGTCATAGCC GAATAGCCTC 
     7621 TCCACCCAAG CGGCCGGAGA ACCTGCGTGC AATCCATCTT GTTCAATCAT GCGAAACGAT 
     7681 CCTCATCCTG TCTCTTGATC GATCTTTGCA AAAGCCTAGG CCTCCAAAAA AGCCTCCTCA 
     7741 CTACTTCTGG AATAGCTCAG AGGCCGAGGC GGCCTCGGCC TCTGCATAAA TAAAAAAAAT 
     7801 TAGTCAGCCA TGGGGCGGAG AATGGGCGGA ACTGGGCGGA GTTAGGGGCG GGATGGGCGG 
     7861 AGTTAGGGGC GGGACTATGG TTGCTGACTA ATTGAGATGC ATGCTTTGCA TACTTCTGCC 
     7921 TGCTGGGGAG CCTGGGGACT TTCCACACCT GGTTGCTGAC TAATTGAGAT GCATGCTTTG 
     7981 CATACTTCTG CCTGCTGGGG AGCCTGGGGA CTTTCCACAC CCTAACTGAC ACACATTCCA 
     8041 CAGCTGGTTC TTTCCGCCTC AGGACTCTTC CTTTTTCAAT ATTATTGAAG CATTTATCAG 
     8101 GGTTATTGTC TCATGAGCGG ATACATATTT GAATGTATTT AGAAAAATAA ACAAATAGGG 
     8161 GTTCCGCGCA CATTTCCCCG AAAAGTGCCA CCTGACGCGC CCTGTAGCGG CGCATTAAGC 
     8221 GCGGCGGGTG TGGTGGTTAC GCGCAGCGTG ACCGCTACAC TTGCCAGCGC CCTAGCGCCC 
     8281 GCTCCTTTCG CTTTCTTCCC TTCCTTTCTC GCCACGTTCG CCGGCTTTCC CCGTCAAGCT 
     8341 CTAAATCGGG GGCTCCCTTT AGGGTTCCGA TTTAGTGCTT TACGGCACCT CGACCCCAAA 
     8401 AAACTTGATT AGGGTGATGG TTCACGTAGT GGGCCATCGC CCTGATAGAC GGTTTTTCGC 
     8461 CCTTTGACGT TGGAGTCCAC GTTCTTTAAT AGTGGACTCT TGTTCCAAAC TGGAACAACA 
     8521 CTCAACCCTA TCTCGGTCTA TTCTTTTGAT TTATAAGGGA TTTTGCCGAT TTCGGCCTAT 
     8581 TGGTTAAAAA ATGAGCTGAT TTAACAAAAA TTTAACGCGA ATTTT
//