LOCUS       RC213531                8880 bp    DNA     circular  SYN 
DEFINITION  SOS1 (Myc-DDK-tagged)-Human son of sevenless homolog 1 (Drosophila) (SOS1)
ACCESSION   NM_005633
VERSION     NM_005633.2
SOURCE      synthetic construct 
FEATURES    Location/Qualifiers 
     misc_feature    201..924 
                     /label="CMV promoter"  
     misc_feature    839..856 
                     /label="Restriction Enzyme"  
     misc_feature    953..971 
                     /label="T7 promoter"  
     misc_feature    5037..5128 
                     /label="Myc-DDK"  
     misc_feature    5158..5743 
                     /label="PolyA signal"  
     misc_feature    5215..5234 
                     /label="Restriction Enzyme"  
     misc_feature    6019..6691 
                     /label="ColE1"  
     misc_feature    7121..7915 
                     /label="Kan/Neo"  
     misc_feature    7950..8252 
                     /label="SV40 ori"  
     misc_feature    8338..8815 
                     /label="f1 ori"  
     misc_feature    1020..1028 
                     /label="Restriction Enzyme"  
     misc_feature    5019..5025 
                     /label="Restriction Enzyme"  
     misc_feature    1020..5019 
                     /label="SOS1"  
ORIGIN                      
        1 AACAAAATAT TAACGCTTAC AATTTCCATT CGCCATTCAG GCTGCGCAAC TGTTGGGAAG 
       61 GGCGATCGGT GCGGGCCTCT TCGCTATTAC GCCAGCTGGC GAAAGGGGGA TGTGCTGCAA 
      121 GGCGATTAAG TTGGGTAACG CCAGGGTTTT CCCAGTCACG ACGTTGTAAA ACGACGGCCA 
      181 GTGCCAAGCT GATCTATACA TTGAATCAAT ATTGGCAATT AGCCATATTA GTCATTGGTT 
      241 ATATAGCATA AATCAATATT GGCTATTGGC CATTGCATAC GTTGTATCTA TATCATAATA 
      301 TGTACATTTA TATTGGCTCA TGTCCAATAT GACCGCCATG TTGACATTGA TTATTGACTA 
      361 GTTATTAATA GTAATCAATT ACGGGGTCAT TAGTTCATAG CCCATATATG GAGTTCCGCG 
      421 TTACATAACT TACGGTAAAT GGCCCGCCTG GCTGACCGCC CAACGACCCC CGCCCATTGA 
      481 CGTCAATAAT GACGTATGTT CCCATAGTAA CGCCAATAGG GACTTTCCAT TGACGTCAAT 
      541 GGGTGGAGTA TTTACGGTAA ACTGCCCACT TGGCAGTACA TCAAGTGTAT CATATGCCAA 
      601 GTCCGCCCCC TATTGACGTC AATGACGGTA AATGGCCCGC CTGGCATTAT GCCCAGTACA 
      661 TGACCTTACG GGACTTTCCT ACTTGGCAGT ACATCTACGT ATTAGTCATC GCTATTACCA 
      721 TGGTGATGCG GTTTTGGCAG TACACCAATG GGCGTGGATA GCGGTTTGAC TCACGGGGAT 
      781 TTCCAAGTCT CCACCCCATT GACGTCAATG GGAGTTTGTT TTGGCACCAA AATCAACGGG 
      841 ACTTTCCAAA ATGTCGTAAT AACCCCGCCC CGTTGACGCA AATGGGCGGT AGGCGTGTAC 
      901 GGTGGGAGGT CTATATAAGC AGAGCTCGTT TAGTGAACCG TCAGAATTTT GTAATACGAC 
      961 TCACTATAGG GCGGCCGGGA ATTCGTCGAC TGGATCCGGT ACCGAGGAGA TCTGCCGCCG 
     1021 CGATCGCCAT GCAGGCGCAG CAGCTGCCCT ACGAGTTTTT CAGCGAAGAG AACGCGCCCA 
     1081 AGTGGCGGGG ACTACTGGTG CCTGCGCTGA AAAAGGTCCA GGGGCAAGTT CATCCTACTC 
     1141 TCGAGTCTAA TGATGATGCT CTTCAGTATG TTGAAGAATT AATTTTGCAA TTATTAAATA 
     1201 TGCTATGCCA AGCTCAGCCC CGAAGTGCTT CAGATGTAGA GGAACGTGTT CAAAAAAGTT 
     1261 TCCCTCATCC AATTGATAAA TGGGCAATAG CTGATGCCCA ATCAGCTATT GAAAAGAGGA 
     1321 AGCGAAGAAA CCCTTTATCT CTCCCAGTAG AAAAAATTCA TCCTTTATTA AAGGAGGTCC 
     1381 TAGGTTATAA AATTGACCAC CAGGTTTCTG TTTACATAGT AGCAGTCTTA GAATACATTT 
     1441 CTGCAGACAT TTTAAAGCTG GTTGGGAATT ATGTAAGAAA TATACGGCAT TATGAAATTA 
     1501 CAAAACAAGA TATTAAAGTG GCAATGTGTG CTGACAAGGT ATTGATGGAT ATGTTTCATC 
     1561 AAGATGTAGA AGATATTAAT ATATTATCTT TAACTGACGA AGAGCCTTCC ACCTCAGGAG 
     1621 AACAAACTTA CTATGATTTG GTAAAAGCAT TTATGGCAGA AATTCGACAA TATATAAGGG 
     1681 AACTAAATCT AATTATAAAA GTTTTTAGAG AGCCCTTTGT CTCCAATTCA AAATTGTTTT 
     1741 CAGCTAATGA TGTAGAAAAT ATATTTAGTC GCATAGTAGA TATACATGAA CTTAGTGTAA 
     1801 AGTTACTGGG CCATATAGAA GATACAGTAG AAATGACAGA TGAAGGCAGT CCCCATCCAC 
     1861 TAGTAGGAAG CTGCTTTGAA GACTTAGCAG AGGAACTGGC ATTTGATCCA TATGAATCGT 
     1921 ATGCTCGAGA TATTTTGCGA CCTGGTTTTC ATGATCGTTT CCTTAGTCAG TTATCAAAGC 
     1981 CTGGGGCAGC ACTTTATTTG CAGTCAATAG GCGAAGGTTT CAAAGAAGCT GTTCAATATG 
     2041 TTTTACCCAG GCTGCTTCTG GCCCCTGTTT ACCACTGTCT CCATTACTTT GAACTTTTGA 
     2101 AGCAGTTAGA AGAAAAAAGT GAAGATCAAG AAGACAAGGA ATGTTTAAAA CAAGCAATAA 
     2161 CAGCTTTGCT TAATGTTCAG AGTGGTATGG AAAAAATATG TTCTAAAAGT CTTGCAAAAC 
     2221 GAAGACTGAG TGAATCTGCA TGTCGGTTTT ATAGTCAGCA AATGAAGGGG AAACAACTAG 
     2281 CAATCAAGAA GATGAACGAG ATTCAGAAGA ATATTGATGG TTGGGAGGGA AAAGACATTG 
     2341 GACAGTGTTG TAATGAATTT ATAATGGAAG GAACTCTTAC ACGTGTAGGA GCCAAACATG 
     2401 AGAGACACAT ATTTCTCTTT GATGGCTTAA TGATTTGCTG TAAATCAAAT CATGGGCAGC 
     2461 CAAGACTTCC TGGTGCTAGC AATGCAGAAT ATCGTCTTAA AGAAAAGTTT TTTATGCGAA 
     2521 AGGTACAAAT TAATGATAAA GATGACACCA ATGAATACAA GCATGCTTTT GAAATAATTT 
     2581 TAAAAGATGA AAATAGTGTT ATATTTTCTG CCAAGTCAGC TGAAGAGAAA AACAATTGGA 
     2641 TGGCAGCATT GATATCTTTA CAGTACCGGA GTACACTGGA AAGGATGCTT GATGTAACAA 
     2701 TGCTACAGGA AGAGAAAGAG GAGCAGATGA GGCTGCCTAG TGCTGATGTT TATAGATTTG 
     2761 CAGAGCCTGA CTCTGAAGAG AATATTATAT TTGAAGAGAA CATGCAGCCC AAGGCTGGAA 
     2821 TTCCAATTAT CAAAGCAGGA ACTGTTATTA AACTTATAGA GAGGCTTACG TACCATATGT 
     2881 ACGCAGATCC CAATTTTGTT CGGACATTTC TTACAACATA CAGATCCTTT TGCAAACCTC 
     2941 AAGAACTACT GAGTCTTATA ATAGAAAGGT TTGAAATTCC AGAGCCTGAG CCAACAGAAG 
     3001 CTGATCGCAT AGCTATAGAG AATGGAGATC AACCCTTGAG TGCAGAACTG AAAAGATTTA 
     3061 GAAAAGAATA TATACAGCCT GTGCAACTGC GAGTATTAAA TGTATGTCGG CACTGGGTAG 
     3121 AGCACCACTT CTATGATTTT GAAAGAGATG CATATCTTTT GCAACGAATG GAAGAATTTA 
     3181 TTGGAACAGT AAGAGGTAAA GCAATGAAAA AATGGGTTGA ATCCATCACT AAAATAATCC 
     3241 AAAGGAAAAA AATTGCAAGA GACAATGGAC CAGGTCATAA TATTACATTT CAGAGTTCAC 
     3301 CTCCCACAGT TGAGTGGCAT ATAAGCAGAC CTGGGCACAT AGAGACTTTT GACCTGCTCA 
     3361 CCTTACACCC AATAGAAATT GCTCGACAAC TCACTTTACT TGAATCAGAT CTATACCGAG 
     3421 CTGTACAGCC ATCAGAATTA GTTGGAAGTG TGTGGACAAA AGAAGACAAA GAAATTAACT 
     3481 CTCCTAATCT TCTGAAAATG ATTCGACATA CCACCAACCT CACTCTGTGG TTTGAGAAAT 
     3541 GTATTGTAGA AACTGAAAAT TTAGAAGAAA GAGTAGCTGT GGTGAGTCGA ATTATTGAGA 
     3601 TTCTACAAGT CTTTCAAGAG TTGAACAACT TTAATGGTGT CCTTGAGGTT GTCAGTGCTA 
     3661 TGAATTCATC ACCTGTTTAC AGACTAGACC ACACATTTGA GCAAATACCA AGTCGCCAGA 
     3721 AGAAAATTTT AGAAGAAGCT CATGAATTGA GTGAAGATCA CTATAAGAAA TATTTGGCAA 
     3781 AACTCAGGTC TATTAATCCA CCATGTGTGC CTTTCTTTGG AATTTATCTC ACTAATATCT 
     3841 TGAAAACAGA AGAAGGCAAC CCTGAGGTCC TAAAAAGACA TGGAAAAGAG CTTATAAACT 
     3901 TTAGCAAAAG GAGGAAAGTA GCAGAAATAA CAGGAGAGAT CCAGCACTAC CAAAATCAGC 
     3961 CTTACTGTTT ACGAGTAGAA TCAGATATCA AAAGGTTCTT TGAAAACTTG AATCCGATGG 
     4021 GAAATAGCAT GGAGAAGGAA TTTACAGATT ATCTTTTCAA CAAATCCCTA GAAATAGAAC 
     4081 CACGAAACCC TAAGCCTCTC CCAAGATTTC CAAAAAAATA TAGCTATCCC CTAAAATCTC 
     4141 CTGGTGTTCG TCCATCAAAC CCAAGACCAG GTACCATGAG GCATCCCACA CCTCTGCAGC 
     4201 AGGAGCCAAG GAAAATTAGT TATAGTAGGA TCCCTGAAAG TGAAACAGAA AGTACAGCAT 
     4261 CTGCACCAAA TTCTCCAAGA ACACCGTTAA CACCTCCGCC TGCTTCTGGT GCTTCCAGTA 
     4321 CCACAGATGT TTGCAGTGTA TTTGATTCCG ATCATTCGAG CCCTTTTCAC TCAAGCAATG 
     4381 ATACCGTCTT TATCCAAGTT ACTCTGCCCC ATGGCCCAAG ATCTGCTTCT GTATCATCTA 
     4441 TAAGTTTAAC CAAAGGCACT GATGAAGTGC CTGTCCCTCC TCCTGTTCCT CCACGAAGAC 
     4501 GACCAGAATC TGCCCCAGCA GAATCTTCAC CATCTAAGAT TATGTCTAAG CATTTGGACA 
     4561 GTCCCCCAGC CATTCCTCCT AGGCAACCCA CATCAAAAGC CTATTCACCA CGATATTCAA 
     4621 TATCAGACCG GACCTCTATC TCAGACCCTC CTGAAAGCCC TCCCTTATTA CCACCACGAG 
     4681 AACCTGTGAG GACACCTGAT GTTTTCTCAA GCTCACCACT ACATCTCCAA CCTCCCCCTT 
     4741 TGGGCAAAAA AAGTGACCAT GGCAATGCCT TCTTCCCAAA CAGCCCTTCC CCCTTTACAC 
     4801 CACCTCCTCC TCAAACACCT TCTCCTCACG GCACAAGAAG GCATCTGCCA TCACCACCAT 
     4861 TGACACAAGA AGTGGACCTT CATTCCATTG CTGGGCCGCC TGTTCCTCCA CGACAAAGCA 
     4921 CTTCTCAACA TATCCCTAAA CTCCCTCCAA AAACTTACAA AAGGGAGCAC ACACACCCAT 
     4981 CCATGCACAG AGATGGACCA CCACTGTTGG AGAATGCCCA TTCTTCCACG CGTACGCGGC 
     5041 CGCTCGAGCA GAAACTCATC TCAGAAGAGG ATCTGGCAGC AAATGATATC CTGGATTACA 
     5101 AGGATGACGA CGATAAGGTT TAAACGGCCG GCCGCGGTCA TAGCTGTTTC CTGAACAGAT 
     5161 CCCGGGTGGC ATCCCTGTGA CCCCTCCCCA GTGCCTCTCC TGGCCCTGGA AGTTGCCACT 
     5221 CCAGTGCCCA CCAGCCTTGT CCTAATAAAA TTAAGTTGCA TCATTTTGTC TGACTAGGTG 
     5281 TCCTTCTATA ATATTATGGG GTGGAGGGGG GTGGTATGGA GCAAGGGGCA AGTTGGGAAG 
     5341 ACAACCTGTA GGGCCTGCGG GGTCTATTGG GAACCAAGCT GGAGTGCAGT GGCACAATCT 
     5401 TGGCTCACTG CAATCTCCGC CTCCTGGGTT CAAGCGATTC TCCTGCCTCA GCCTCCCGAG 
     5461 TTGTTGGGAT TCCAGGCATG CATGACCAGG CTCAGCTAAT TTTTGTTTTT TTGGTAGAGA 
     5521 CGGGGTTTCA CCATATTGGC CAGGCTGGTC TCCAACTCCT AATCTCAGGT GATCTACCCA 
     5581 CCTTGGCCTC CCAAATTGCT GGGATTACAG GCGTGAACCA CTGCTCCCTT CCCTGTCCTT 
     5641 CTGATTTTAA AATAACTATA CCAGCAGGAG GACGTCCAGA CACAGCATAG GCTACCTGGC 
     5701 CATGCCCAAC CGGTGGGACA TTTGAGTTGC TTGCTTGGCA CTGTCCTCTC ATGCGTTGGG 
     5761 TCCACTCAGT AGATGCCTGT TGAATTGGGT ACGCGGCCAG CGGCGAGCGG TATCAGCTCA 
     5821 CTCAAAGGCG GTAATACGGT TATCCACAGA ATCAGGGGAT AACGCAGGAA AGAACATGTG 
     5881 AGCAAAAGGC CAGCAAAAGG CCAGGAACCG TAAAAAGGCC GCGTTGCTGG CGTTTTTCCA 
     5941 TAGGCTCCGC CCCCCTGACG AGCATCACAA AAATCGACGC TCAAGTCAGA GGTGGCGAAA 
     6001 CCCGACAGGA CTATAAAGAT ACCAGGCGTT TCCCCCTGGA AGCTCCCTCG TGCGCTCTCC 
     6061 TGTTCCGACC CTGCCGCTTA CCGGATACCT GTCCGCCTTT CTCCCTTCGG GAAGCGTGGC 
     6121 GCTTTCTCAT AGCTCACGCT GTAGGTATCT CAGTTCGGTG TAGGTCGTTC GCTCCAAGCT 
     6181 GGGCTGTGTG CACGAACCCC CCGTTCAGCC CGACCGCTGC GCCTTATCCG GTAACTATCG 
     6241 TCTTGAGTCC AACCCGGTAA GACACGACTT ATCGCCACTG GCAGCAGCCA CTGGTAACAG 
     6301 GATTAGCAGA GCGAGGTATG TAGGCGGTGC TACAGAGTTC TTGAAGTGGT GGCCTAACTA 
     6361 CGGCTACACT AGAAGAACAG TATTTGGTAT CTGCGCTCTG CTGAAGCCAG TTACCTTCGG 
     6421 AAAAAGAGTT GGTAGCTCTT GATCCGGCAA ACAAACCACC GCTGGTAGCG GTGGTTTTTT 
     6481 TGTTTGCAAG CAGCAGATTA CGCGCAGAAA AAAAGGATCT CAAGAAGATC CTTTGATCTT 
     6541 TTCTACGGGG TCTGACGCTC AGTGGAACGA AAACTCACGT TAAGGGATTT TGGTCATGAG 
     6601 ATTATCAAAA AGGATCTTCA CCTAGATCCT TTTAAATTAA AAATGAAGTT TTAAATCAAT 
     6661 CTAAAGTATA TATGAGTAAC CTGAGGCTAT GGCAGGGCCT GCCGCCCCGA CGTTGGCTGC 
     6721 GAGCCCTGGG CCTTCACCCG AACTTGGGGG GTGGGGTGGG GAAAAGGAAG AAACGCGGGC 
     6781 GTATTGGCCC CAATGGGGTC TCGGTGGGGT ATCGACAGAG TGCCAGCCCT GGGACCGAAC 
     6841 CCCGCGTTTA TGAACAAACG ACCCAACACC GTGCGTTTTA TTCTGTCTTT TTATTGCCGT 
     6901 CATAGCGCGG GTTCCTTCCG GTATTGTCTC CTTCCGTGTT TCAGTTAGCC TCCCCCTAGG 
     6961 GTGGGCGAAG AACTCCAGCA TGAGATCCCC GCGCTGGAGG ATCATCCAGC CGGCGTCCCG 
     7021 GAAAACGATT CCGAAGCCCA ACCTTTCATA GAAGGCGGCG GTGGAATCGA AATCTCGTGA 
     7081 TGGCAGGTTG GGCGTCGCTT GGTCGGTCAT TTCGAACCCC AGAGTCCCGC TCAGAAGAAC 
     7141 TCGTCAAGAA GGCGATAGAA GGCGATGCGC TGCGAATCGG GAGCGGCGAT ACCGTAAAGC 
     7201 ACGAGGAAGC GGTCAGCCCA TTCGCCGCCA AGCTCTTCAG CAATATCACG GGTAGCCAAC 
     7261 GCTATGTCCT GATAGCGATC CGCCACACCC AGCCGGCCAC AGTCGATGAA TCCAGAAAAG 
     7321 CGGCCATTTT CCACCATGAT ATTCGGCAAG CAGGCATCGC CATGGGTCAC GACGAGATCC 
     7381 TCGCCGTCGG GCATGCTCGC CTTGAGCCTG GCGAACAGTT CGGCTGGCGC GAGCCCCTGA 
     7441 TGCTCTTCGT CCAGATCATC CTGATCGACA AGACCGGCTT CCATCCGAGT ACGTGCTCGC 
     7501 TCGATGCGAT GTTTCGCTTG GTGGTCGAAT GGGCAGGTAG CCGGATCAAG CGTATGCAGC 
     7561 CGCCGCATTG CATCAGCCAT GATGGATACT TTCTCGGCAG GAGCAAGGTG AGATGACAGG 
     7621 AGATCCTGCC CCGGCACTTC GCCCAATAGC AGCCAGTCCC TTCCCGCTTC AGTGACAACG 
     7681 TCGAGCACAG CTGCGCAAGG AACGCCCGTC GTGGCCAGCC ACGATAGCCG CGCTGCCTCG 
     7741 TCTTGCAGTT CATTCAGGGC ACCGGACAGG TCGGTCTTGA CAAAAAGAAC CGGGCGCCCC 
     7801 TGCGCTGACA GCCGGAACAC GGCGGCATCA GAGCAGCCGA TTGTCTGTTG TGCCCAGTCA 
     7861 TAGCCGAATA GCCTCTCCAC CCAAGCGGCC GGAGAACCTG CGTGCAATCC ATCTTGTTCA 
     7921 ATCATGCGAA ACGATCCTCA TCCTGTCTCT TGATCGATCT TTGCAAAAGC CTAGGCCTCC 
     7981 AAAAAAGCCT CCTCACTACT TCTGGAATAG CTCAGAGGCC GAGGCGGCCT CGGCCTCTGC 
     8041 ATAAATAAAA AAAATTAGTC AGCCATGGGG CGGAGAATGG GCGGAACTGG GCGGAGTTAG 
     8101 GGGCGGGATG GGCGGAGTTA GGGGCGGGAC TATGGTTGCT GACTAATTGA GATGCATGCT 
     8161 TTGCATACTT CTGCCTGCTG GGGAGCCTGG GGACTTTCCA CACCTGGTTG CTGACTAATT 
     8221 GAGATGCATG CTTTGCATAC TTCTGCCTGC TGGGGAGCCT GGGGACTTTC CACACCCTAA 
     8281 CTGACACACA TTCCACAGCT GGTTCTTTCC GCCTCAGGAC TCTTCCTTTT TCAATATTAT 
     8341 TGAAGCATTT ATCAGGGTTA TTGTCTCATG AGCGGATACA TATTTGAATG TATTTAGAAA 
     8401 AATAAACAAA TAGGGGTTCC GCGCACATTT CCCCGAAAAG TGCCACCTGA CGCGCCCTGT 
     8461 AGCGGCGCAT TAAGCGCGGC GGGTGTGGTG GTTACGCGCA GCGTGACCGC TACACTTGCC 
     8521 AGCGCCCTAG CGCCCGCTCC TTTCGCTTTC TTCCCTTCCT TTCTCGCCAC GTTCGCCGGC 
     8581 TTTCCCCGTC AAGCTCTAAA TCGGGGGCTC CCTTTAGGGT TCCGATTTAG TGCTTTACGG 
     8641 CACCTCGACC CCAAAAAACT TGATTAGGGT GATGGTTCAC GTAGTGGGCC ATCGCCCTGA 
     8701 TAGACGGTTT TTCGCCCTTT GACGTTGGAG TCCACGTTCT TTAATAGTGG ACTCTTGTTC 
     8761 CAAACTGGAA CAACACTCAA CCCTATCTCG GTCTATTCTT TTGATTTATA AGGGATTTTG 
     8821 CCGATTTCGG CCTATTGGTT AAAAAATGAG CTGATTTAAC AAAAATTTAA CGCGAATTTT 
//