LOCUS       RC213520L1                8188 bp    DNA     circular  SYN 
DEFINITION  KLHL15 (Myc-DDK-tagged)-Human kelch-like 15 (Drosophila) (KLHL15)
ACCESSION   NM_030624
VERSION     null
SOURCE      synthetic construct 
FEATURES    Location/Qualifiers 
     misc_feature    6479..6659 
                     /label="3'LTR"  
     misc_feature    833..1013 
                     /label="5'LTR"  
     misc_feature    7387..8046 
                     /label="CAT(Cam-r)"  
     misc_feature    2620..3234 
                     /label="CMV promoter"  
     misc_feature    6818..7386 
                     /label="ColE1"  
     misc_feature    5354..5374 
                     /label="LR50 primer"  
     misc_feature    5151..5222 
                     /label="Myc-DDK"  
     misc_feature    3252..3271 
                     /label="T7 promoter"  
     misc_feature    3221..3237 
                     /label="V2 primer"  
     misc_feature    5363..5954 
                     /label="WPRE"  
     misc_feature    3320..3328 
                     /label="Restriction Enzyme"  
     misc_feature    5133..5139 
                     /label="Restriction Enzyme"  
     misc_feature    3320..5133 
                     /label="KLHL15"  
ORIGIN                      
        1 GTCGACGGAT CGGGAGATCT CCCGATCCCC TATGGTGCAC TCTCAGTACA ATCTGCTCTG 
       61 ATGCCGCATA GTTAAGCCAG TATCTGCTCC CTGCTTGTGT GTTGGAGGTC GCTGAGTAGT 
      121 GCGCGAGCAA AATTTAAGCT ACAACAAGGC AAGGCTTGAC CGACAATTGC ATGAAGAATC 
      181 TGCTTAGGGT TAGGCGTTTT GCGCTGCTTC GCGATGTACG GGCCAGATAT CGCGTTGACA 
      241 TTGATTATTG ACTAGTTATT AATAGTAATC AATTACGGGG TCATTAGTTC ATAGCCCATA 
      301 TATGGAGTTC CGCGTTACAT AACTTACGGT AAATGGCCCG CCTGGCTGAC CGCCCAACGA 
      361 CCCCCGCCCA TTGACGTCAA TAATGACGTA TGTTCCCATA GTAACGCCAA TAGGGACTTT 
      421 CCATTGACGT CAATGGGTGG AGTATTTACG GTAAACTGCC CACTTGGCAG TACATCAAGT 
      481 GTATCATATG CCAAGTACGC CCCCTATTGA CGTCAATGAC GGTAAATGGC CCGCCTGGCA 
      541 TTATGCCCAG TACATGACCT TATGGGACTT TCCTACTTGG CAGTACATCT ACGTATTAGT 
      601 CATCGCTATT ACCATGGTGA TGCGGTTTTG GCAGTACATC AATGGGCGTG GATAGCGGTT 
      661 TGACTCACGG GGATTTCCAA GTCTCCACCC CATTGACGTC AATGGGAGTT TGTTTTGGCA 
      721 CCAAAATCAA CGGGACTTTC CAAAATGTCG TAACAACTCC GCCCCATTGA CGCAAATGGG 
      781 CGGTAGGCGT GTACGGTGGG AGGTCTATAT AAGCAGCGCG TTTTGCCTGT ACTGGGTCTC 
      841 TCTGGTTAGA CCAGATCTGA GCCTGGGAGC TCTCTGGCTA ACTAGGGAAC CCACTGCTTA 
      901 AGCCTCAATA AAGCTTGCCT TGAGTGCTTC AAGTAGTGTG TGCCCGTCTG TTGTGTGACT 
      961 CTGGTAACTA GAGATCCCTC AGACCCTTTT AGTCAGTGTG GAAAATCTCT AGCAGTGGCG 
     1021 CCCGAACAGG GACTTGAAAG CGAAAGGGAA ACCAGAGGAG CTCTCTCGAC GCAGGACTCG 
     1081 GCTTGCTGAA GCGCGCACGG CAAGAGGCGA GGGGCGGCGA CTGGTGAGTA CGCCAAAAAT 
     1141 TTTGACTAGC GGAGGCTAGA AGGAGAGAGA TGGGTGCGAG AGCGTCAGTA TTAAGCGGGG 
     1201 GAGAATTAGA TCGCGATGGG AAAAAATTCG GTTAAGGCCA GGGGGAAAGA AAAAATATAA 
     1261 ATTAAAACAT ATAGTATGGG CAAGCAGGGA GCTAGAACGA TTCGCAGTTA ATCCTGGCCT 
     1321 GTTAGAAACA TCAGAAGGCT GTAGACAAAT ACTGGGACAG CTACAACCAT CCCTTCAGAC 
     1381 AGGATCAGAA GAACTTAGAT CATTATATAA TACAGTAGCA ACCCTCTATT GTGTGCATCA 
     1441 AAGGATAGAG ATAAAAGACA CCAAGGAAGC TTTAGACAAG ATAGAGGAAG AGCAAAACAA 
     1501 AAGTAAGACC ACCGCACAGC AAGCGGCCGG CCGCTGATCT TCAGACCTGG AGGAGGAGAT 
     1561 ATGAGGGACA ATTGGAGAAG TGAATTATAT AAATATAAAG TAGTAAAAAT TGAACCATTA 
     1621 GGAGTAGCAC CCACCAAGGC AAAGAGAAGA GTGGTGCAGA GAGAAAAAAG AGCAGTGGGA 
     1681 ATAGGAGCTT TGTTCCTTGG GTTCTTGGGA GCAGCAGGAA GCACTATGGG CGCAGCGTCA 
     1741 ATGACGCTGA CGGTACAGGC CAGACAATTA TTGTCTGGTA TAGTGCAGCA GCAGAACAAT 
     1801 TTGCTGAGGG CTATTGAGGC GCAACAGCAT CTGTTGCAAC TCACAGTCTG GGGCATCAAG 
     1861 CAGCTCCAGG CAAGAATCCT GGCTGTGGAA AGATACCTAA AGGATCAACA GCTCCTGGGG 
     1921 ATTTGGGGTT GCTCTGGAAA ACTCATTTGC ACCACTGCTG TGCCTTGGAA TGCTAGTTGG 
     1981 AGTAATAAAT CTCTGGAACA GATTTGGAAT CACACGACCT GGATGGAGTG GGACAGAGAA 
     2041 ATTAACAATT ACACAAGCTT AATACACTCC TTAATTGAAG AATCGCAAAA CCAGCAAGAA 
     2101 AAGAATGAAC AAGAATTATT GGAATTAGAT AAATGGGCAA GTTTGTGGAA TTGGTTTAAC 
     2161 ATAACAAATT GGCTGTGGTA TATAAAATTA TTCATAATGA TAGTAGGAGG CTTGGTAGGT 
     2221 TTAAGAATAG TTTTTGCTGT ACTTTCTATA GTGAATAGAG TTAGGCAGGG ATATTCACCA 
     2281 TTATCGTTTC AGACCCACCT CCCAACCCCG AGGGGACCCG ACAGGCCCGA AGGAATAGAA 
     2341 GAAGAAGGTG GAGAGAGAGA CAGAGACAGA TCCATTCGAT TAGTGAACGG ATCGGCACTG 
     2401 CGTGCGCCAA TTCTGCAGAC AAATGGCAGT ATTCATCCAC AATTTTAAAA GAAAAGGGGG 
     2461 GATTGGGGGG TACAGTGCAG GGGAAAGAAT AGTAGACATA ATAGCAACAG ACATACAAAC 
     2521 TAAAGAATTA CAAAAACAAA TTACAAAAAT TCAAAATTTT CGGGTTTATT ACAGGGACAG 
     2581 CAGAGATCCA GTTTGGTTAG TACCGGGCCC GCTCTAGACA TGTCCAATAT GACCGCCATG 
     2641 TTGACATTGA TTATTGACTA GTTATTAATA GTAATCAATT ACGGGGTCAT TAGTTCATAG 
     2701 CCCATATATG GAGTTCCGCG TTACATAACT TACGGTAAAT GGCCCGCCTG GCTGACCGCC 
     2761 CAACGACCCC CGCCCATTGA CGTCAATAAT GACGTATGTT CCCATAGTAA CGCCAATAGG 
     2821 GACTTTCCAT TGACGTCAAT GGGTGGAGTA TTTACGGTAA ACTGCCCACT TGGCAGTACA 
     2881 TCAAGTGTAT CATATGCCAA GTCCGCCCCC TATTGACGTC AATGACGGTA AATGGCCCGC 
     2941 CTGGCATTAT GCCCAGTACA TGACCTTACG GGACTTTCCT ACTTGGCAGT ACATCTACGT 
     3001 ATTAGTCATC GCTATTACCA TGGTGATGCG GTTTTGGCAG TACACCAATG GGCGTGGATA 
     3061 GCGGTTTGAC TCACGGGGAT TTCCAAGTCT CCACCCCATT GACGTCAATG GGAGTTTGTT 
     3121 TTGGCACCAA AATCAACGGG ACTTTCCAAA ATGTCGTAAT AACCCCGCCC CGTTGACGCA 
     3181 AATGGGCGGT AGGCGTGTAC GGTGGGAGGT CTATATAAGC AGAGCTCGTT TAGTGAACCG 
     3241 TCAGAATTTT GTAATACGAC TCACTATAGG GCGGCCGGGA ATTCGTCGAC TGGATCCGGT 
     3301 ACCGAGGAGA TCTGCCGCCG CGATCGCCAT GGCAGGGGAC GTGGAAGGAT TCTGTTCCTC 
     3361 CATCCACGAC ACCAGTGTCT CTGCTGGGTT CAGAGCACTG TATGAGGAGG GATTGCTTCT 
     3421 TGATGTCACT CTGGTTATTG AAGATCATCA GTTCCAGGCC CATAAAGCAC TCTTGGCCAC 
     3481 CCAGAGTGAT TACTTCAGAA TTATGTTTAC TGCAGACATG AGGGAACGAG ATCAGGACAA 
     3541 AATTCATTTA AAAGGTCTAA CAGCTACCGG TTTCAGCCAT GTCCTGCAAT TTATGTACTA 
     3601 TGGAACTATA GAGCTGAGTA TGAATACCGT TCATGAGATT CTTCAGGCTG CCATGTATGT 
     3661 TCAACTTATA GAAGTGGTGA AGTTCTGCTG CTCTTTTCTC TTAGCGAAGA TCTGCCTAGA 
     3721 AAATTGTGCA GAAATTATGA GACTCTTAGA TGATTTCGGC GTAAACATCG AGGGAGTCAG 
     3781 GGAGAAGTTA GACACCTTTC TGCTAGACAA CTTTGTGCCA CTCATGTCTA GGCCTGACTT 
     3841 TCTGTCCTAT CTGAGCTTTG AGAAGCTCAT GTCTTACTTG GATAATGATC ATCTGAGCAG 
     3901 GTTCCCAGAG ATAGAGCTGT ACGAGGCTGT GCAGTCTTGG CTGCGGCATG ATAGAAGACG 
     3961 CTGGAGACAT ACCGATACCA TCATTCAGAA TATCCGGTTT TGCTTGATGA CCCCAACCAG 
     4021 CGTTTTTGAG AAGGTTAAGA CATCAGAATT TTATAGATAC TCCCGACAGC TCCGTTACGA 
     4081 AGTTGACCAA GCATTGAATT ACTTTCAGAA TGTTCACCAG CAGCCTTTGT TGGATATGAA 
     4141 GTCAAGCCGC ATCCGTTCTG CAAAACCGCA AACTACAGTA TTTCGAGGAA TGATTGGACA 
     4201 TAGCATGGTT AACAGTAAAA TACTTCTCTT AAAGAAACCA AGAGTCTGGT GGGAGCTAGA 
     4261 AGGCCCACAA GTACCTCTGC GACCTGACTG CCTTGCTATC GTCAATAATT TTGTGTTCCT 
     4321 GTTAGGCGGG GAAGAGCTGG GCCCGGATGG TGAATTCCAT GCTTCTTCCA AAGTATTCAG 
     4381 GTATGACCCG AGACAGAACT CCTGGCTGCA GATGGCAGAT ATGTCTGTAC CACGCTCTGA 
     4441 ATTTGCTGTA GGTGTTATTG GGAAGTTTAT TTACGCCGTA GCAGGCAGAA CCAGAGATGA 
     4501 GACTTTCTAT TCAACTGAGA GATATGACAT CACCAACGAT AAATGGGAAT TTGTGGATCC 
     4561 TTATCCAGTT AACAAATATG GACATGAGGG GACAGTGCTC AATAACAAAT TGTTTATCAC 
     4621 CGGTGGAATC ACCTCATCTT CCACCTCCAA GCAAGTGTGC GTGTTTGACC CCAGCAAAGA 
     4681 AGGGACCATA GAACAACGGA CCAGGAGAAC TCAAGTGGTT ACCAACTGTT GGGAGAATAA 
     4741 GAGCAAGATG AATTACGCGA GATGCTTTCA CAAGATGATT TCTTACAATG GCAAGCTTTA 
     4801 TGTCTTCGGT GGTGTCTGTG TGATCTTGAG GGCCTCTTTC GAATCTCAGG GATGCCCTTC 
     4861 TACAGAAGTA TACAACCCAG AGACTGATCA GTGGACCATC TTGGCATCCA TGCCGATTGG 
     4921 TAGAAGTGGC CATGGTGTGA CTGTGCTGGA CAAACAAATA ATGGTTCTTG GAGGCCTTTG 
     4981 TTATAATGGT CATTACAGCG ATTCCATCCT CACTTTTGAT CCGGATGAAA ACAAGTGGAA 
     5041 GGAAGATGAG TACCCTCGGA TGCCCTGCAA GCTGGATGGT TTACAAGTAT GCAACCTGCA 
     5101 TTTTCCGGAC TATGTACTGG ATGAGGTCAG GCGTTGCAAC TACGCGTACG CGGCCGCTCG 
     5161 AGCAGAAACT CATCTCAGAA GAGGATCTGG CAGCAAATGA TATCCTGGAT TACAAGGATG 
     5221 ACGACGATAA GGTTTAAACG GCCGGCCGCG GTCTGTACAA GTAGGATTCG TCGAGGGACC 
     5281 TAATAACTTC GTATAGCATA CATTATACGA AGTTATACAT GTTTAAGGGT TCCGGTTCCA 
     5341 CTAGGTACAA TTCGATATCA AGCTTATCGA TAATCAACCT CTGGATTACA AAATTTGTGA 
     5401 AAGATTGACT GGTATTCTTA ACTATGTTGC TCCTTTTACG CTATGTGGAT ACGCTGCTTT 
     5461 AATGCCTTTG TATCATGCTA TTGCTTCCCG TATGGCTTTC ATTTTCTCCT CCTTGTATAA 
     5521 ATCCTGGTTG CTGTCTCTTT ATGAGGAGTT GTGGCCCGTT GTCAGGCAAC GTGGCGTGGT 
     5581 GTGCACTGTG TTTGCTGACG CAACCCCCAC TGGTTGGGGC ATTGCCACCA CCTGTCAGCT 
     5641 CCTTTCCGGG ACTTTCGCTT TCCCCCTCCC TATTGCCACG GCGGAACTCA TCGCCGCCTG 
     5701 CCTTGCCCGC TGCTGGACAG GGGCTCGGCT GTTGGGCACT GACAATTCCG TGGTGTTGTC 
     5761 GGGGAAATCA TCGTCCTTTC CTTGGCTGCT CGCCTGTGTT GCCACCTGGA TTCTGCGCGG 
     5821 GACGTCCTTC TGCTACGTCC CTTCGGCCCT CAATCCAGCG GACCTTCCTT CCCGCGGCCT 
     5881 GCTGCCGGCT CTGCGGCCTC TTCCGCGTCT TCGCCTTCGC CCTCAGACGA GTCGGATCTC 
     5941 CCTTTGGGCC GCCTCCCCGC ATCGATACCG TCGACCTCGA TCGAGACCTA GAAAAACATG 
     6001 GAGCAATCAC AAGTAGCAAT ACAGCAGCTA CCAATGCTGA TTGTGCCTGG CTAGAAGCAC 
     6061 AAGAGGAGGA GGAGGTGGGT TTTCCAGTCA CACCTCAGGT ACCTTTAAGA CCAATGACTT 
     6121 ACAAGGCAGC TGTAGATCTT AGCCACTTTT TAAAAGAAAA GGGGGGACTG GAAGGGCTAA 
     6181 TTCACTCCCA ACGAAGACAA GATATCCTTG ATCTGTGGAT CTACCACACA CAAGGCTACT 
     6241 TCCCTGATTG GCAGAACTAC ACACCAGGGC CAGGGATCAG ATATCCACTG ACCTTTGGAT 
     6301 GGTGCTACAA GCTAGTACCA GTTGAGCAAG AGAAGGTAGA AGAAGCCAAT GAAGGAGAGA 
     6361 ACACCCGCTT GTTACACCCT GTGAGCCTGC ATGGGATGGA TGACCCGGAG AGAGAAGTAT 
     6421 TAGAGTGGAG GTTTGACAGC CGCCTAGCAT TTCATCACAT GGCCCGAGAG CTGCATCCGG 
     6481 ACTGTACTGG GTCTCTCTGG TTAGACCAGA TCTGAGCCTG GGAGCTCTCT GGCTAACTAG 
     6541 GGAACCCACT GCTTAAGCCT CAATAAAGCT TGCCTTGAGT GCTTCAAGTA GTGTGTGCCC 
     6601 GTCTGTTGTG TGACTCTGGT AACTAGAGAT CCCTCAGACC CTTTTAGTCA GTGTGGAAAA 
     6661 TCTCTAGCAG CATGTGAGCA AAAGGCCAGC AAAAGGCCAG GAACCGTAAA AAGGCCGCGT 
     6721 TGCTGGCGTT TTTCCATAGG CTCCGCCCCC CTGACGAGCA TCACAAAAAT CGACGCTCAA 
     6781 GTCAGAGGTG GCGAAACCCG ACAGGACTAT AAAGATACCA GGCGTTTCCC CCTGGAAGCT 
     6841 CCCTCGTGCG CTCTCCTGTT CCGACCCTGC CGCTTACCGG ATACCTGTCC GCCTTTCTCC 
     6901 CTTCGGGAAG CGTGGCGCTT TCTCATAGCT CACGCTGTAG GTATCTCAGT TCGGTGTAGG 
     6961 TCGTTCGCTC CAAGCTGGGC TGTGTGCACG AACCCCCCGT TCAGCCCGAC CGCTGCGCCT 
     7021 TATCCGGTAA CTATCGTCTT GAGTCCAACC CGGTAAGACA CGACTTATCG CCACTGGCAG 
     7081 CAGCCACTGG TAACAGGATT AGCAGAGCGA GGTATGTAGG CGGTGCTACA GAGTTCTTGA 
     7141 AGTGGTGGCC TAACTACGGC TACACTAGAA GAACAGTATT TGGTATCTGC GCTCTGCTGA 
     7201 AGCCAGTTAC CTTCGGAAAA AGAGTTGGTA GCTCTTGATC CGGCAAACAA ACCACCGCTG 
     7261 GTAGCGGTGG TTTTTTTGTT TGCAAGCAGC AGATTACGCG CAGAAAAAAA GGATCTCAAG 
     7321 AAGATCCTTT GATCTTTTCT ACGGGGTCTG ACGCTCAGTG GAACGAAAAC TCACGTTAAG 
     7381 GGATTTTGGT CATGATTACG CCCCGCCCTG CCACTCATCG CAGTACTGTT GTAATTCATT 
     7441 AAGCATTCTG CCGACATGGA AGCCATCACA AACGGCATGA TGAACCTGAA TCGCCAGCGG 
     7501 CATCAGCACC TTGTCGCCTT GCGTATAATA TTTGCCCATG GTGAAAACGG GGGCGAAGAA 
     7561 GTTGTCCATA TTGGCCACGT TTAAATCAAA ACTGGTGAAA CTCACCCAGG GATTGGCTGA 
     7621 GACGAAAAAC ATATTCTCAA TAAACCCTTT AGGGAAATAG GCCAGGTTTT CACCGTAACA 
     7681 CGCCACATCT TGCGAATATA TGTGTAGAAA CTGCCGGAAA TCGTCGTGGT ATTCACTCCA 
     7741 GAGCGATGAA AACGTTTCAG TTTGCTCATG GAAAACGGTG TAACAAGGGT GAACACTATC 
     7801 CCATATCACC AGCTCACCGT CTTTCATTGC CATACGGAAC TCCGGATGAG CATTCATCAG 
     7861 GCGGGCAAGA ATGTGAATAA AGGCCGGATA AAACTTGTGC TTATTTTTCT TTACGGTCTT 
     7921 TAAAAAGGCC GTAATATCCA GCTGAACGGT CTGGTTATAG GTACATTGAG CAACTGACTG 
     7981 AAATGCCTCA AAATGTTCTT TACGATGCCA TTGGGATATA TCAACGGTGG TATATCCAGT 
     8041 GATTTTTTTC TCCATACTCT TCCTTTTTCA ATATTATTGA AGCATTTATC AGGGTTATTG 
     8101 TCTCATGAGC GGATACATAT TTGAATGTAT TTAGAAAAAT AAACAAATAG GGGTCCCGCG 
     8161 CACATTTCCC CGAAAAGTGC CACCTGAC
//