LOCUS       RC213379                8496 bp    DNA     circular  SYN 
DEFINITION  APAF1 (Myc-DDK-tagged)-Human apoptotic peptidase activating factor 1 (APAF1), transcript variant 4
ACCESSION   NM_181868
VERSION     NM_181868.1
SOURCE      synthetic construct 
FEATURES    Location/Qualifiers 
     misc_feature    201..924 
                     /label="CMV promoter"  
     misc_feature    839..856 
                     /label="Restriction Enzyme"  
     misc_feature    953..971 
                     /label="T7 promoter"  
     misc_feature    4653..4744 
                     /label="Myc-DDK"  
     misc_feature    4774..5359 
                     /label="PolyA signal"  
     misc_feature    4831..4850 
                     /label="Restriction Enzyme"  
     misc_feature    5635..6307 
                     /label="ColE1"  
     misc_feature    6737..7531 
                     /label="Kan/Neo"  
     misc_feature    7566..7868 
                     /label="SV40 ori"  
     misc_feature    7954..8431 
                     /label="f1 ori"  
     misc_feature    1020..1028 
                     /label="Restriction Enzyme"  
     misc_feature    4635..4641 
                     /label="Restriction Enzyme"  
     misc_feature    1020..4635 
                     /label="APAF1"  
ORIGIN                      
        1 AACAAAATAT TAACGCTTAC AATTTCCATT CGCCATTCAG GCTGCGCAAC TGTTGGGAAG 
       61 GGCGATCGGT GCGGGCCTCT TCGCTATTAC GCCAGCTGGC GAAAGGGGGA TGTGCTGCAA 
      121 GGCGATTAAG TTGGGTAACG CCAGGGTTTT CCCAGTCACG ACGTTGTAAA ACGACGGCCA 
      181 GTGCCAAGCT GATCTATACA TTGAATCAAT ATTGGCAATT AGCCATATTA GTCATTGGTT 
      241 ATATAGCATA AATCAATATT GGCTATTGGC CATTGCATAC GTTGTATCTA TATCATAATA 
      301 TGTACATTTA TATTGGCTCA TGTCCAATAT GACCGCCATG TTGACATTGA TTATTGACTA 
      361 GTTATTAATA GTAATCAATT ACGGGGTCAT TAGTTCATAG CCCATATATG GAGTTCCGCG 
      421 TTACATAACT TACGGTAAAT GGCCCGCCTG GCTGACCGCC CAACGACCCC CGCCCATTGA 
      481 CGTCAATAAT GACGTATGTT CCCATAGTAA CGCCAATAGG GACTTTCCAT TGACGTCAAT 
      541 GGGTGGAGTA TTTACGGTAA ACTGCCCACT TGGCAGTACA TCAAGTGTAT CATATGCCAA 
      601 GTCCGCCCCC TATTGACGTC AATGACGGTA AATGGCCCGC CTGGCATTAT GCCCAGTACA 
      661 TGACCTTACG GGACTTTCCT ACTTGGCAGT ACATCTACGT ATTAGTCATC GCTATTACCA 
      721 TGGTGATGCG GTTTTGGCAG TACACCAATG GGCGTGGATA GCGGTTTGAC TCACGGGGAT 
      781 TTCCAAGTCT CCACCCCATT GACGTCAATG GGAGTTTGTT TTGGCACCAA AATCAACGGG 
      841 ACTTTCCAAA ATGTCGTAAT AACCCCGCCC CGTTGACGCA AATGGGCGGT AGGCGTGTAC 
      901 GGTGGGAGGT CTATATAAGC AGAGCTCGTT TAGTGAACCG TCAGAATTTT GTAATACGAC 
      961 TCACTATAGG GCGGCCGGGA ATTCGTCGAC TGGATCCGGT ACCGAGGAGA TCTGCCGCCG 
     1021 CGATCGCCAT GGATGCAAAA GCTCGAAATT GTTTGCTTCA ACATAGAGAA GCTCTGGAAA 
     1081 AGGACATCAA GACATCCTAC ATCATGGATC ACATGATTAG TGATGGATTT TTAACAATAT 
     1141 CAGAAGAGGA AAAAGTAAGA AATGAGCCCA CTCAACAGCA AAGAGCAGCT ATGCTGATTA 
     1201 AAATGATACT TAAAAAAGAT AATGATTCCT ACGTATCATT CTACAATGCT CTACTACATG 
     1261 AAGGATATAA AGATCTTGCT GCCCTTCTCC ATGATGGCAT TCCTGTTGTC TCTTCTTCCA 
     1321 GTGGTAAAGA TTCAGTTAGT GGAATAACTT CGTATGTAAG GACAGTCCTG TGTGAAGGTG 
     1381 GAGTACCACA GAGGCCAGTT GTTTTTGTCA CAAGGAAGAA GCTGGTGAAT GCAATTCAGC 
     1441 AGAAGCTCTC CAAATTGAAA GGTGAACCAG GATGGGTCAC CATACATGGA ATGGCAGGCT 
     1501 GTGGGAAGTC TGTATTAGCT GCAGAAGCTG TTAGAGATCA TTCCCTTTTA GAAGGTTGTT 
     1561 TCCCAGGGGG AGTGCATTGG GTTTCAGTTG GGAAACAAGA CAAATCTGGG CTTCTGATGA 
     1621 AACTGCAGAA TCTTTGCACA CGGTTGGATC AGGATGAGAG TTTTTCCCAG AGGCTTCCAC 
     1681 TTAATATTGA AGAGGCTAAA GACCGTCTCC GCATTCTGAT GCTTCGCAAA CACCCAAGGT 
     1741 CTCTCTTGAT CTTGGATGAT GTTTGGGACT CTTGGGTGTT GAAAGCTTTT GACAGTCAGT 
     1801 GTCAGATTCT TCTTACAACC AGAGACAAGA GTGTTACAGA TTCAGTAATG GGTCCTAAAT 
     1861 ATGTAGTCCC TGTGGAGAGT TCCTTAGGAA AGGAAAAAGG ACTTGAAATT TTATCCCTTT 
     1921 TTGTTAATAT GAAGAAGGCA GATTTGCCAG AACAAGCTCA TAGTATTATA AAAGAATGTA 
     1981 AAGGCTCTCC CCTTGTAGTA TCTTTAATTG GTGCACTTTT ACGTGATTTT CCCAATCGCT 
     2041 GGGAGTACTA CCTCAAACAG CTTCAGAATA AGCAGTTTAA GAGAATAAGG AAATCTTCGT 
     2101 CTTATGATTA TGAGGCTCTA GATGAAGCCA TGTCTATAAG TGTTGAAATG CTCAGAGAAG 
     2161 ACATCAAAGA TTATTACACA GATCTTTCCA TCCTTCAGAA GGACGTTAAG GTGCCTACAA 
     2221 AGGTGTTATG TATTCTCTGG GACATGGAAA CTGAAGAAGT TGAAGACATA CTGCAGGAGT 
     2281 TTGTAAATAA GTCTCTTTTA TTCTGTGATC GGAATGGAAA GTCGTTTCGT TATTATTTAC 
     2341 ATGATCTTCA AGTAGATTTT CTTACAGAGA AGAATTGCAG CCAGCTTCAG GATCTACATA 
     2401 AGAAGATAAT CACTCAGTTT CAGAGATATC ACCAGCCGCA TACTCTTTCA CCAGATCAGG 
     2461 AAGACTGTAT GTATTGGTAC AACTTTCTGG CCTATCACAT GGCCAGTGCC AAGATGCACA 
     2521 AGGAACTTTG TGCTTTAATG TTTTCCCTGG ATTGGATTAA AGCAAAAACA GAACTTGTAG 
     2581 GCCCTGCTCA TCTGATTCAT GAATTTGTGG AATACAGACA TATACTAGAT GAAAAGGATT 
     2641 GTGCAGTCAG TGAGAATTTT CAGGAGTTTT TATCTTTAAA TGGACACCTT CTTGGACGAC 
     2701 AGCCATTTCC TAATATTGTA CAACTGGGTC TCTGTGAGCC GGAAACTTCA GAAGTTTATC 
     2761 AGCAAGCTAA GCTGCAGGCC AAGCAGGAGG TCGATAATGG AATGCTTTAC CTGGAATGGA 
     2821 TAAACAAAAA AAACATCACG AATCTTTCCC GCTTAGTTGT CCGCCCCCAC ACAGATGCTG 
     2881 TTTACCATGC CTGCTTTTCT GAGGATGGTC AGAGAATAGC TTCTTGTGGA GCTGATAAAA 
     2941 CCTTACAGGT GTTCAAAGCT GAAACAGGAG AGAAACTTCT AGAAATCAAG GCTCATGAGG 
     3001 ATGAAGTGCT TTGTTGTGCA TTCTCTACAG ATGACAGATT TATAGCAACC TGCTCAGTGG 
     3061 ATAAAAAAGT GAAGATTTGG AATTCTATGA CTGGGGAACT AGTACACACC TATGATGAGC 
     3121 ACTCAGAGCA AGTCAATTGC TGCCATTTCA CCAACAGTAG TCATCATCTT CTCTTAGCCA 
     3181 CTGGGTCAAG TGACTGCTTC CTCAAACTTT GGGATTTGAA TCAAAAAGAA TGTCGAAATA 
     3241 CCATGTTTGG TCATACAAAT TCAGTCAATC ACTGCAGATT TTCACCAGAT GATAAGCTTT 
     3301 TGGCTAGTTG TTCAGCTGAT GGAACCTTAA AGCTTTGGGA TGCGACATCA GCAAATGAGA 
     3361 GGAAAAGCAT TAATGTGAAA CAGTTCTTCC TAAATTTGGA GGACCCTCAA GAGGATATGG 
     3421 AAGTGATAGT GAAGTGTTGT TCGTGGTCTG CTGATGGTGC AAGGATAATG GTGGCAGCAA 
     3481 AAAATAAAAT CTTTTTGTGG AATACAGACT CACGTTCAAA GGTGGCTGAT TGCAGAGGAC 
     3541 ATTTAAGTTG GGTTCATGGT GTGATGTTTT CTCCTGATGG ATCATCATTT TTGACATCTT 
     3601 CTGATGACCA GACAATCAGG CTCTGGGAGA CAAAGAAAGT ATGTAAGAAC TCTGCTGTAA 
     3661 TGTTAAAGCA AGAAGTAGAT GTTGTGTTTC AAGAAAATGA AGTGATGGTC CTTGCAGTTG 
     3721 ACCATATAAG ACGTCTGCAA CTCATTAATG GAAGAACAGG TCAGATTGAT TATCTGACTG 
     3781 AAGCTCAAGT TAGCTGCTGT TGCTTAAGTC CACATCTTCA GTACATTGCA TTTGGAGATG 
     3841 AAAATGGAGC CATTGAGATT TTAGAACTTG TAAACAATAG AATCTTCCAG TCCAGGTTTC 
     3901 AGCACAAGAA AACTGTATGG CACATCCAGT TCACAGCCGA TGAGAAGACT CTTATTTCAA 
     3961 GTTCTGATGA TGCTGAAATT CAGGTATGGA ATTGGCAATT GGACAAATGT ATCTTTCTAC 
     4021 GAGGCCATCA GGAAACAGTG AAAGACTTTA GACTCTTGAA AAATTCAAGA CTGCTTTCTT 
     4081 GGTCATTTGA TGGAACAGTG AAGGTATGGA ATATTATTAC TGGAAATAAA GAAAAAGACT 
     4141 TTGTCTGTCA CCAGGGTACA GTACTTTCTT GTGACATTTC TCACGATGCT ACCAAGTTTT 
     4201 CATCTACCTC TGCTGACAAG ACTGCAAAGA TCTGGAGTTT TGATCTCCTT TTGCCACTTC 
     4261 ATGAATTGAG GGGCCACAAC GGCTGTGTGC GCTGCTCTGC CTTCTCTGTG GACAGTACCC 
     4321 TGCTGGCAAC GGGAGATGAC AATGGAGAAA TCAGGATATG GAATGTCTCA AACGGTGAGC 
     4381 TTCTTCATTT GTGTGCTCCG CTTTCAGAAG AAGGAGCTGC TACCCATGGA GGCTGGGTGA 
     4441 CTGACCTTTG CTTTTCTCCA GATGGCAAAA TGCTTATCTC TGCTGGAGGA TATATTAAGT 
     4501 GGTGGAACGT TGTCACTGGG GAATCCTCAC AGACCTTCTA CACAAATGGA ACCAATCTTA 
     4561 AGAAAATACA CGTGTCCCCT GACTTCAAAA CATATGTGAC TGTGGATAAT CTTGGTATTT 
     4621 TATATATTTT ACAGACTTTA GAAACGCGTA CGCGGCCGCT CGAGCAGAAA CTCATCTCAG 
     4681 AAGAGGATCT GGCAGCAAAT GATATCCTGG ATTACAAGGA TGACGACGAT AAGGTTTAAA 
     4741 CGGCCGGCCG CGGTCATAGC TGTTTCCTGA ACAGATCCCG GGTGGCATCC CTGTGACCCC 
     4801 TCCCCAGTGC CTCTCCTGGC CCTGGAAGTT GCCACTCCAG TGCCCACCAG CCTTGTCCTA 
     4861 ATAAAATTAA GTTGCATCAT TTTGTCTGAC TAGGTGTCCT TCTATAATAT TATGGGGTGG 
     4921 AGGGGGGTGG TATGGAGCAA GGGGCAAGTT GGGAAGACAA CCTGTAGGGC CTGCGGGGTC 
     4981 TATTGGGAAC CAAGCTGGAG TGCAGTGGCA CAATCTTGGC TCACTGCAAT CTCCGCCTCC 
     5041 TGGGTTCAAG CGATTCTCCT GCCTCAGCCT CCCGAGTTGT TGGGATTCCA GGCATGCATG 
     5101 ACCAGGCTCA GCTAATTTTT GTTTTTTTGG TAGAGACGGG GTTTCACCAT ATTGGCCAGG 
     5161 CTGGTCTCCA ACTCCTAATC TCAGGTGATC TACCCACCTT GGCCTCCCAA ATTGCTGGGA 
     5221 TTACAGGCGT GAACCACTGC TCCCTTCCCT GTCCTTCTGA TTTTAAAATA ACTATACCAG 
     5281 CAGGAGGACG TCCAGACACA GCATAGGCTA CCTGGCCATG CCCAACCGGT GGGACATTTG 
     5341 AGTTGCTTGC TTGGCACTGT CCTCTCATGC GTTGGGTCCA CTCAGTAGAT GCCTGTTGAA 
     5401 TTGGGTACGC GGCCAGCGGC GAGCGGTATC AGCTCACTCA AAGGCGGTAA TACGGTTATC 
     5461 CACAGAATCA GGGGATAACG CAGGAAAGAA CATGTGAGCA AAAGGCCAGC AAAAGGCCAG 
     5521 GAACCGTAAA AAGGCCGCGT TGCTGGCGTT TTTCCATAGG CTCCGCCCCC CTGACGAGCA 
     5581 TCACAAAAAT CGACGCTCAA GTCAGAGGTG GCGAAACCCG ACAGGACTAT AAAGATACCA 
     5641 GGCGTTTCCC CCTGGAAGCT CCCTCGTGCG CTCTCCTGTT CCGACCCTGC CGCTTACCGG 
     5701 ATACCTGTCC GCCTTTCTCC CTTCGGGAAG CGTGGCGCTT TCTCATAGCT CACGCTGTAG 
     5761 GTATCTCAGT TCGGTGTAGG TCGTTCGCTC CAAGCTGGGC TGTGTGCACG AACCCCCCGT 
     5821 TCAGCCCGAC CGCTGCGCCT TATCCGGTAA CTATCGTCTT GAGTCCAACC CGGTAAGACA 
     5881 CGACTTATCG CCACTGGCAG CAGCCACTGG TAACAGGATT AGCAGAGCGA GGTATGTAGG 
     5941 CGGTGCTACA GAGTTCTTGA AGTGGTGGCC TAACTACGGC TACACTAGAA GAACAGTATT 
     6001 TGGTATCTGC GCTCTGCTGA AGCCAGTTAC CTTCGGAAAA AGAGTTGGTA GCTCTTGATC 
     6061 CGGCAAACAA ACCACCGCTG GTAGCGGTGG TTTTTTTGTT TGCAAGCAGC AGATTACGCG 
     6121 CAGAAAAAAA GGATCTCAAG AAGATCCTTT GATCTTTTCT ACGGGGTCTG ACGCTCAGTG 
     6181 GAACGAAAAC TCACGTTAAG GGATTTTGGT CATGAGATTA TCAAAAAGGA TCTTCACCTA 
     6241 GATCCTTTTA AATTAAAAAT GAAGTTTTAA ATCAATCTAA AGTATATATG AGTAACCTGA 
     6301 GGCTATGGCA GGGCCTGCCG CCCCGACGTT GGCTGCGAGC CCTGGGCCTT CACCCGAACT 
     6361 TGGGGGGTGG GGTGGGGAAA AGGAAGAAAC GCGGGCGTAT TGGCCCCAAT GGGGTCTCGG 
     6421 TGGGGTATCG ACAGAGTGCC AGCCCTGGGA CCGAACCCCG CGTTTATGAA CAAACGACCC 
     6481 AACACCGTGC GTTTTATTCT GTCTTTTTAT TGCCGTCATA GCGCGGGTTC CTTCCGGTAT 
     6541 TGTCTCCTTC CGTGTTTCAG TTAGCCTCCC CCTAGGGTGG GCGAAGAACT CCAGCATGAG 
     6601 ATCCCCGCGC TGGAGGATCA TCCAGCCGGC GTCCCGGAAA ACGATTCCGA AGCCCAACCT 
     6661 TTCATAGAAG GCGGCGGTGG AATCGAAATC TCGTGATGGC AGGTTGGGCG TCGCTTGGTC 
     6721 GGTCATTTCG AACCCCAGAG TCCCGCTCAG AAGAACTCGT CAAGAAGGCG ATAGAAGGCG 
     6781 ATGCGCTGCG AATCGGGAGC GGCGATACCG TAAAGCACGA GGAAGCGGTC AGCCCATTCG 
     6841 CCGCCAAGCT CTTCAGCAAT ATCACGGGTA GCCAACGCTA TGTCCTGATA GCGATCCGCC 
     6901 ACACCCAGCC GGCCACAGTC GATGAATCCA GAAAAGCGGC CATTTTCCAC CATGATATTC 
     6961 GGCAAGCAGG CATCGCCATG GGTCACGACG AGATCCTCGC CGTCGGGCAT GCTCGCCTTG 
     7021 AGCCTGGCGA ACAGTTCGGC TGGCGCGAGC CCCTGATGCT CTTCGTCCAG ATCATCCTGA 
     7081 TCGACAAGAC CGGCTTCCAT CCGAGTACGT GCTCGCTCGA TGCGATGTTT CGCTTGGTGG 
     7141 TCGAATGGGC AGGTAGCCGG ATCAAGCGTA TGCAGCCGCC GCATTGCATC AGCCATGATG 
     7201 GATACTTTCT CGGCAGGAGC AAGGTGAGAT GACAGGAGAT CCTGCCCCGG CACTTCGCCC 
     7261 AATAGCAGCC AGTCCCTTCC CGCTTCAGTG ACAACGTCGA GCACAGCTGC GCAAGGAACG 
     7321 CCCGTCGTGG CCAGCCACGA TAGCCGCGCT GCCTCGTCTT GCAGTTCATT CAGGGCACCG 
     7381 GACAGGTCGG TCTTGACAAA AAGAACCGGG CGCCCCTGCG CTGACAGCCG GAACACGGCG 
     7441 GCATCAGAGC AGCCGATTGT CTGTTGTGCC CAGTCATAGC CGAATAGCCT CTCCACCCAA 
     7501 GCGGCCGGAG AACCTGCGTG CAATCCATCT TGTTCAATCA TGCGAAACGA TCCTCATCCT 
     7561 GTCTCTTGAT CGATCTTTGC AAAAGCCTAG GCCTCCAAAA AAGCCTCCTC ACTACTTCTG 
     7621 GAATAGCTCA GAGGCCGAGG CGGCCTCGGC CTCTGCATAA ATAAAAAAAA TTAGTCAGCC 
     7681 ATGGGGCGGA GAATGGGCGG AACTGGGCGG AGTTAGGGGC GGGATGGGCG GAGTTAGGGG 
     7741 CGGGACTATG GTTGCTGACT AATTGAGATG CATGCTTTGC ATACTTCTGC CTGCTGGGGA 
     7801 GCCTGGGGAC TTTCCACACC TGGTTGCTGA CTAATTGAGA TGCATGCTTT GCATACTTCT 
     7861 GCCTGCTGGG GAGCCTGGGG ACTTTCCACA CCCTAACTGA CACACATTCC ACAGCTGGTT 
     7921 CTTTCCGCCT CAGGACTCTT CCTTTTTCAA TATTATTGAA GCATTTATCA GGGTTATTGT 
     7981 CTCATGAGCG GATACATATT TGAATGTATT TAGAAAAATA AACAAATAGG GGTTCCGCGC 
     8041 ACATTTCCCC GAAAAGTGCC ACCTGACGCG CCCTGTAGCG GCGCATTAAG CGCGGCGGGT 
     8101 GTGGTGGTTA CGCGCAGCGT GACCGCTACA CTTGCCAGCG CCCTAGCGCC CGCTCCTTTC 
     8161 GCTTTCTTCC CTTCCTTTCT CGCCACGTTC GCCGGCTTTC CCCGTCAAGC TCTAAATCGG 
     8221 GGGCTCCCTT TAGGGTTCCG ATTTAGTGCT TTACGGCACC TCGACCCCAA AAAACTTGAT 
     8281 TAGGGTGATG GTTCACGTAG TGGGCCATCG CCCTGATAGA CGGTTTTTCG CCCTTTGACG 
     8341 TTGGAGTCCA CGTTCTTTAA TAGTGGACTC TTGTTCCAAA CTGGAACAAC ACTCAACCCT 
     8401 ATCTCGGTCT ATTCTTTTGA TTTATAAGGG ATTTTGCCGA TTTCGGCCTA TTGGTTAAAA 
     8461 AATGAGCTGA TTTAACAAAA ATTTAACGCG AATTTT
//