LOCUS       RC213359                9216 bp    DNA     circular  SYN 
DEFINITION  CD109 (Myc-DDK-tagged)-Human CD109 molecule (CD109), transcript variant 1
ACCESSION   NM_133493
VERSION     NM_133493.1
SOURCE      synthetic construct 
FEATURES    Location/Qualifiers 
     misc_feature    201..924 
                     /label="CMV promoter"  
     misc_feature    839..856 
                     /label="Restriction Enzyme"  
     misc_feature    953..971 
                     /label="T7 promoter"  
     misc_feature    5373..5464 
                     /label="Myc-DDK"  
     misc_feature    5494..6079 
                     /label="PolyA signal"  
     misc_feature    5551..5570 
                     /label="Restriction Enzyme"  
     misc_feature    6355..7027 
                     /label="ColE1"  
     misc_feature    7457..8251 
                     /label="Kan/Neo"  
     misc_feature    8286..8588 
                     /label="SV40 ori"  
     misc_feature    8674..9151 
                     /label="f1 ori"  
     misc_feature    1020..1028 
                     /label="Restriction Enzyme"  
     misc_feature    5355..5361 
                     /label="Restriction Enzyme"  
     misc_feature    1020..5355 
                     /label="CD109"  
ORIGIN                      
        1 AACAAAATAT TAACGCTTAC AATTTCCATT CGCCATTCAG GCTGCGCAAC TGTTGGGAAG 
       61 GGCGATCGGT GCGGGCCTCT TCGCTATTAC GCCAGCTGGC GAAAGGGGGA TGTGCTGCAA 
      121 GGCGATTAAG TTGGGTAACG CCAGGGTTTT CCCAGTCACG ACGTTGTAAA ACGACGGCCA 
      181 GTGCCAAGCT GATCTATACA TTGAATCAAT ATTGGCAATT AGCCATATTA GTCATTGGTT 
      241 ATATAGCATA AATCAATATT GGCTATTGGC CATTGCATAC GTTGTATCTA TATCATAATA 
      301 TGTACATTTA TATTGGCTCA TGTCCAATAT GACCGCCATG TTGACATTGA TTATTGACTA 
      361 GTTATTAATA GTAATCAATT ACGGGGTCAT TAGTTCATAG CCCATATATG GAGTTCCGCG 
      421 TTACATAACT TACGGTAAAT GGCCCGCCTG GCTGACCGCC CAACGACCCC CGCCCATTGA 
      481 CGTCAATAAT GACGTATGTT CCCATAGTAA CGCCAATAGG GACTTTCCAT TGACGTCAAT 
      541 GGGTGGAGTA TTTACGGTAA ACTGCCCACT TGGCAGTACA TCAAGTGTAT CATATGCCAA 
      601 GTCCGCCCCC TATTGACGTC AATGACGGTA AATGGCCCGC CTGGCATTAT GCCCAGTACA 
      661 TGACCTTACG GGACTTTCCT ACTTGGCAGT ACATCTACGT ATTAGTCATC GCTATTACCA 
      721 TGGTGATGCG GTTTTGGCAG TACACCAATG GGCGTGGATA GCGGTTTGAC TCACGGGGAT 
      781 TTCCAAGTCT CCACCCCATT GACGTCAATG GGAGTTTGTT TTGGCACCAA AATCAACGGG 
      841 ACTTTCCAAA ATGTCGTAAT AACCCCGCCC CGTTGACGCA AATGGGCGGT AGGCGTGTAC 
      901 GGTGGGAGGT CTATATAAGC AGAGCTCGTT TAGTGAACCG TCAGAATTTT GTAATACGAC 
      961 TCACTATAGG GCGGCCGGGA ATTCGTCGAC TGGATCCGGT ACCGAGGAGA TCTGCCGCCG 
     1021 CGATCGCCAT GCAGGGCCCA CCGCTCCTGA CCGCCGCCCA CCTCCTCTGC GTGTGCACCG 
     1081 CCGCGCTGGC CGTGGCTCCC GGGCCTCGGT TTCTGGTGAC AGCCCCAGGG ATCATCAGGC 
     1141 CCGGAGGAAA TGTGACTATT GGGGTGGAGC TTCTGGAACA CTGCCCTTCA CAGGTGACTG 
     1201 TGAAGGCGGA GCTGCTCAAG ACAGCATCAA ACCTCACTGT CTCTGTCCTG GAAGCAGAAG 
     1261 GAGTCTTTGA AAAAGGCTCT TTTAAGACAC TTACTCTTCC ATCACTACCT CTGAACAGTG 
     1321 CAGATGAGAT TTATGAGCTA CGTGTAACCG GACGTACCCA GGATGAGATT TTATTCTCTA 
     1381 ATAGTACCCG CTTATCATTT GAGACCAAGA GAATATCTGT CTTCATTCAA ACAGACAAGG 
     1441 CCTTATACAA GCCAAAGCAA GAAGTGAAGT TTCGCATTGT TACACTCTTC TCAGATTTTA 
     1501 AGCCTTACAA AACCTCTTTA AACATTCTCA TTAAGGACCC CAAATCAAAT TTGATCCAAC 
     1561 AGTGGTTGTC ACAACAAAGT GATCTTGGAG TCATTTCCAA AACTTTTCAG CTATCTTCCC 
     1621 ATCCAATACT TGGTGACTGG TCTATTCAAG TTCAAGTGAA TGACCAGACA TATTATCAAT 
     1681 CATTTCAGGT TTCAGAATAT GTATTACCAA AATTTGAAGT GACTTTGCAG ACACCATTAT 
     1741 ATTGTTCTAT GAATTCTAAG CATTTAAATG GTACCATCAC GGCAAAGTAT ACATATGGGA 
     1801 AGCCAGTGAA AGGAGACGTA ACGCTTACAT TTTTACCTTT ATCCTTTTGG GGAAAGAAGA 
     1861 AAAATATTAC AAAAACATTT AAGATAAATG GATCTGCAAA CTTCTCTTTT AATGATGAAG 
     1921 AGATGAAAAA TGTAATGGAT TCTTCAAATG GACTTTCTGA ATACCTGGAT CTATCTTCCC 
     1981 CTGGACCAGT AGAAATTTTA ACCACAGTGA CAGAATCAGT TACAGGTATT TCAAGAAATG 
     2041 TAAGCACTAA TGTGTTCTTC AAGCAACATG ATTACATCAT TGAGTTTTTT GATTATACTA 
     2101 CTGTCTTGAA GCCATCTCTC AACTTCACAG CCACTGTGAA GGTAACTCGT GCTGATGGCA 
     2161 ACCAACTGAC TCTTGAAGAA AGAAGAAATA ATGTAGTCAT AACAGTGACA CAGAGAAACT 
     2221 ATACTGAGTA CTGGAGCGGA TCTAACAGTG GAAATCAGAA AATGGAAGCT GTTCAGAAAA 
     2281 TAAATTATAC TGTCCCCCAA AGTGGAACTT TTAAGATTGA ATTCCCAATC CTGGAGGATT 
     2341 CCAGTGAGCT ACAGTTGAAG GCCTATTTCC TTGGTAGTAA AAGTAGCATG GCAGTTCATA 
     2401 GTCTGTTTAA GTCTCCTAGT AAGACATACA TCCAACTAAA AACAAGAGAT GAAAATATAA 
     2461 AGGTGGGATC GCCTTTTGAG TTGGTGGTTA GTGGCAACAA ACGATTGAAG GAGTTAAGCT 
     2521 ATATGGTAGT ATCCAGGGGA CAGTTGGTGG CTGTAGGAAA ACAAAATTCA ACAATGTTCT 
     2581 CTTTAACACC AGAAAATTCT TGGACTCCAA AAGCCTGTGT AATTGTGTAT TATATTGAAG 
     2641 ATGATGGGGA AATTATAAGT GATGTTCTAA AAATTCCTGT TCAGCTTGTT TTTAAAAATA 
     2701 AGATAAAGCT ATATTGGAGT AAAGTGAAAG CTGAACCATC TGAGAAAGTC TCTCTTAGGA 
     2761 TCTCTGTGAC ACAGCCTGAC TCCATAGTTG GGATTGTAGC TGTTGACAAA AGTGTGAATC 
     2821 TGATGAATGC CTCTAATGAT ATTACAATGG AAAATGTGGT CCATGAGTTG GAACTTTATA 
     2881 ACACAGGATA TTATTTAGGC ATGTTCATGA ATTCTTTTGC AGTCTTTCAG GAATGTGGAC 
     2941 TCTGGGTATT GACAGATGCA AACCTCACGA AGGATTATAT TGATGGTGTT TATGACAATG 
     3001 CAGAATATGC TGAGAGGTTT ATGGAGGAAA ATGAAGGACA TATTGTAGAT ATTCATGACT 
     3061 TTTCTTTGGG TAGCAGTCCA CATGTCCGAA AGCATTTTCC AGAGACTTGG ATTTGGCTAG 
     3121 ACACCAACAT GGGTTACAGG ATTTACCAAG AATTTGAAGT AACTGTACCT GATTCTATCA 
     3181 CTTCTTGGGT GGCTACTGGT TTTGTGATCT CTGAGGACCT GGGTCTTGGA CTAACAACTA 
     3241 CTCCAGTGGA GCTCCAAGCC TTCCAACCAT TTTTCATTTT TTTGAATCTT CCCTACTCTG 
     3301 TTATCAGAGG TGAAGAATTT GCTTTGGAAA TAACTATATT CAATTATTTG AAAGATGCCA 
     3361 CTGAGGTTAA GGTAATCATT GAGAAAAGTG ACAAATTTGA TATTCTAATG ACTTCAAATG 
     3421 AAATAAATGC CACAGGCCAC CAGCAGACCC TTCTGGTTCC CAGTGAGGAT GGGGCAACTG 
     3481 TTCTTTTTCC CATCAGGCCA ACACATCTGG GAGAAATTCC TATCACAGTC ACAGCTCTTT 
     3541 CACCCACTGC TTCTGATGCT GTCACCCAGA TGATTTTAGT AAAGGCTGAA GGAATAGAAA 
     3601 AATCATATTC ACAATCCATC TTATTAGACT TGACTGACAA TAGGCTACAG AGTACCCTGA 
     3661 AAACTTTGAG TTTCTCATTT CCTCCTAATA CAGTGACTGG CAGTGAAAGA GTTCAGATCA 
     3721 CTGCAATTGG AGATGTTCTT GGTCCTTCCA TCAATGGCTT AGCCTCATTG ATTCGGATGC 
     3781 CTTATGGCTG TGGTGAACAG AACATGATAA ATTTTGCTCC AAATATTTAC ATTTTGGATT 
     3841 ATCTGACTAA AAAGAAACAA CTGACAGATA ATTTGAAAGA AAAAGCTCTT TCATTTATGA 
     3901 GGCAAGGTTA CCAGAGAGAA CTTCTCTATC AGAGGGAAGA TGGCTCTTTC AGTGCTTTTG 
     3961 GGAATTATGA CCCTTCTGGG AGCACTTGGT TGTCAGCTTT TGTTTTAAGA TGTTTCCTTG 
     4021 AAGCCGATCC TTACATAGAT ATTGATCAGA ATGTGTTACA CAGAACATAC ACTTGGCTTA 
     4081 AAGGACATCA GAAATCCAAC GGTGAATTTT GGGATCCAGG AAGAGTGATT CATAGTGAGC 
     4141 TTCAAGGTGG CAATAAAAGT CCAGTAACAC TTACAGCCTA TATTGTAACT TCTCTCCTGG 
     4201 GATATAGAAA GTATCAGCCT AACATTGATG TGCAAGAGTC TATCCATTTT TTGGAGTCTG 
     4261 AATTCAGTAG AGGAATTTCA GACAATTATA CTCTAGCCCT TATAACTTAT GCATTGTCAT 
     4321 CAGTGGGGAG TCCTAAAGCG AAGGAAGCTT TGAATATGCT GACTTGGAGA GCAGAACAAG 
     4381 AAGGTGGCAT GCAATTCTGG GTGTCATCAG AGTCCAAACT TTCTGACTCC TGGCAGCCAC 
     4441 GCTCCCTGGA TATTGAAGTT GCAGCCTATG CACTGCTCTC ACACTTCTTA CAATTTCAGA 
     4501 CTTCTGAGGG AATCCCAATT ATGAGGTGGC TAAGCAGGCA AAGAAATAGC TTGGGTGGTT 
     4561 TTGCATCTAC TCAGGATACC ACTGTGGCTT TAAAGGCTCT GTCTGAATTT GCAGCCCTAA 
     4621 TGAATACAGA AAGGACAAAT ATCCAAGTGA CCGTGACGGG GCCTAGCTCA CCAAGTCCTG 
     4681 TAAAGTTTCT GATTGACACA CACAACCGCT TACTCCTTCA GACAGCAGAG CTTGCTGTGG 
     4741 TACAGCCAAT GGCAGTTAAT ATTTCCGCAA ATGGTTTTGG ATTTGCTATT TGTCAGCTCA 
     4801 ATGTTGTATA TAATGTGAAG GCTTCTGGGT CTTCTAGAAG ACGAAGATCT ATCCAAAATC 
     4861 AAGAAGCCTT TGATTTAGAT GTTGCTGTAA AAGAAAATAA AGATGATCTC AATCATGTGG 
     4921 ATTTGAATGT GTGTACAAGC TTTTCGGGCC CGGGTAGGAG TGGCATGGCT CTTATGGAAG 
     4981 TTAACCTATT AAGTGGCTTT ATGGTGCCTT CAGAAGCAAT TTCTCTGAGC GAGACAGTGA 
     5041 AGAAAGTGGA ATATGATCAT GGAAAACTCA ACCTCTATTT AGATTCTGTA AATGAAACCC 
     5101 AGTTTTGTGT TAATATTCCT GCTGTGAGAA ACTTTAAAGT TTCAAATACC CAAGATGCTT 
     5161 CAGTGTCCAT AGTGGATTAC TATGAGCCAA GGAGACAGGC GGTGAGAAGT TACAACTCTG 
     5221 AAGTGAAGCT GTCCTCCTGT GACCTTTGCA GTGATGTCCA GGGCTGCCGT CCTTGTGAGG 
     5281 ATGGAGCTTC AGGCTCCCAT CATCACTCTT CAGTCATTTT TATTTTCTGT TTCAAGCTTC 
     5341 TGTACTTTAT GGAACTTTGG CTGACGCGTA CGCGGCCGCT CGAGCAGAAA CTCATCTCAG 
     5401 AAGAGGATCT GGCAGCAAAT GATATCCTGG ATTACAAGGA TGACGACGAT AAGGTTTAAA 
     5461 CGGCCGGCCG CGGTCATAGC TGTTTCCTGA ACAGATCCCG GGTGGCATCC CTGTGACCCC 
     5521 TCCCCAGTGC CTCTCCTGGC CCTGGAAGTT GCCACTCCAG TGCCCACCAG CCTTGTCCTA 
     5581 ATAAAATTAA GTTGCATCAT TTTGTCTGAC TAGGTGTCCT TCTATAATAT TATGGGGTGG 
     5641 AGGGGGGTGG TATGGAGCAA GGGGCAAGTT GGGAAGACAA CCTGTAGGGC CTGCGGGGTC 
     5701 TATTGGGAAC CAAGCTGGAG TGCAGTGGCA CAATCTTGGC TCACTGCAAT CTCCGCCTCC 
     5761 TGGGTTCAAG CGATTCTCCT GCCTCAGCCT CCCGAGTTGT TGGGATTCCA GGCATGCATG 
     5821 ACCAGGCTCA GCTAATTTTT GTTTTTTTGG TAGAGACGGG GTTTCACCAT ATTGGCCAGG 
     5881 CTGGTCTCCA ACTCCTAATC TCAGGTGATC TACCCACCTT GGCCTCCCAA ATTGCTGGGA 
     5941 TTACAGGCGT GAACCACTGC TCCCTTCCCT GTCCTTCTGA TTTTAAAATA ACTATACCAG 
     6001 CAGGAGGACG TCCAGACACA GCATAGGCTA CCTGGCCATG CCCAACCGGT GGGACATTTG 
     6061 AGTTGCTTGC TTGGCACTGT CCTCTCATGC GTTGGGTCCA CTCAGTAGAT GCCTGTTGAA 
     6121 TTGGGTACGC GGCCAGCGGC GAGCGGTATC AGCTCACTCA AAGGCGGTAA TACGGTTATC 
     6181 CACAGAATCA GGGGATAACG CAGGAAAGAA CATGTGAGCA AAAGGCCAGC AAAAGGCCAG 
     6241 GAACCGTAAA AAGGCCGCGT TGCTGGCGTT TTTCCATAGG CTCCGCCCCC CTGACGAGCA 
     6301 TCACAAAAAT CGACGCTCAA GTCAGAGGTG GCGAAACCCG ACAGGACTAT AAAGATACCA 
     6361 GGCGTTTCCC CCTGGAAGCT CCCTCGTGCG CTCTCCTGTT CCGACCCTGC CGCTTACCGG 
     6421 ATACCTGTCC GCCTTTCTCC CTTCGGGAAG CGTGGCGCTT TCTCATAGCT CACGCTGTAG 
     6481 GTATCTCAGT TCGGTGTAGG TCGTTCGCTC CAAGCTGGGC TGTGTGCACG AACCCCCCGT 
     6541 TCAGCCCGAC CGCTGCGCCT TATCCGGTAA CTATCGTCTT GAGTCCAACC CGGTAAGACA 
     6601 CGACTTATCG CCACTGGCAG CAGCCACTGG TAACAGGATT AGCAGAGCGA GGTATGTAGG 
     6661 CGGTGCTACA GAGTTCTTGA AGTGGTGGCC TAACTACGGC TACACTAGAA GAACAGTATT 
     6721 TGGTATCTGC GCTCTGCTGA AGCCAGTTAC CTTCGGAAAA AGAGTTGGTA GCTCTTGATC 
     6781 CGGCAAACAA ACCACCGCTG GTAGCGGTGG TTTTTTTGTT TGCAAGCAGC AGATTACGCG 
     6841 CAGAAAAAAA GGATCTCAAG AAGATCCTTT GATCTTTTCT ACGGGGTCTG ACGCTCAGTG 
     6901 GAACGAAAAC TCACGTTAAG GGATTTTGGT CATGAGATTA TCAAAAAGGA TCTTCACCTA 
     6961 GATCCTTTTA AATTAAAAAT GAAGTTTTAA ATCAATCTAA AGTATATATG AGTAACCTGA 
     7021 GGCTATGGCA GGGCCTGCCG CCCCGACGTT GGCTGCGAGC CCTGGGCCTT CACCCGAACT 
     7081 TGGGGGGTGG GGTGGGGAAA AGGAAGAAAC GCGGGCGTAT TGGCCCCAAT GGGGTCTCGG 
     7141 TGGGGTATCG ACAGAGTGCC AGCCCTGGGA CCGAACCCCG CGTTTATGAA CAAACGACCC 
     7201 AACACCGTGC GTTTTATTCT GTCTTTTTAT TGCCGTCATA GCGCGGGTTC CTTCCGGTAT 
     7261 TGTCTCCTTC CGTGTTTCAG TTAGCCTCCC CCTAGGGTGG GCGAAGAACT CCAGCATGAG 
     7321 ATCCCCGCGC TGGAGGATCA TCCAGCCGGC GTCCCGGAAA ACGATTCCGA AGCCCAACCT 
     7381 TTCATAGAAG GCGGCGGTGG AATCGAAATC TCGTGATGGC AGGTTGGGCG TCGCTTGGTC 
     7441 GGTCATTTCG AACCCCAGAG TCCCGCTCAG AAGAACTCGT CAAGAAGGCG ATAGAAGGCG 
     7501 ATGCGCTGCG AATCGGGAGC GGCGATACCG TAAAGCACGA GGAAGCGGTC AGCCCATTCG 
     7561 CCGCCAAGCT CTTCAGCAAT ATCACGGGTA GCCAACGCTA TGTCCTGATA GCGATCCGCC 
     7621 ACACCCAGCC GGCCACAGTC GATGAATCCA GAAAAGCGGC CATTTTCCAC CATGATATTC 
     7681 GGCAAGCAGG CATCGCCATG GGTCACGACG AGATCCTCGC CGTCGGGCAT GCTCGCCTTG 
     7741 AGCCTGGCGA ACAGTTCGGC TGGCGCGAGC CCCTGATGCT CTTCGTCCAG ATCATCCTGA 
     7801 TCGACAAGAC CGGCTTCCAT CCGAGTACGT GCTCGCTCGA TGCGATGTTT CGCTTGGTGG 
     7861 TCGAATGGGC AGGTAGCCGG ATCAAGCGTA TGCAGCCGCC GCATTGCATC AGCCATGATG 
     7921 GATACTTTCT CGGCAGGAGC AAGGTGAGAT GACAGGAGAT CCTGCCCCGG CACTTCGCCC 
     7981 AATAGCAGCC AGTCCCTTCC CGCTTCAGTG ACAACGTCGA GCACAGCTGC GCAAGGAACG 
     8041 CCCGTCGTGG CCAGCCACGA TAGCCGCGCT GCCTCGTCTT GCAGTTCATT CAGGGCACCG 
     8101 GACAGGTCGG TCTTGACAAA AAGAACCGGG CGCCCCTGCG CTGACAGCCG GAACACGGCG 
     8161 GCATCAGAGC AGCCGATTGT CTGTTGTGCC CAGTCATAGC CGAATAGCCT CTCCACCCAA 
     8221 GCGGCCGGAG AACCTGCGTG CAATCCATCT TGTTCAATCA TGCGAAACGA TCCTCATCCT 
     8281 GTCTCTTGAT CGATCTTTGC AAAAGCCTAG GCCTCCAAAA AAGCCTCCTC ACTACTTCTG 
     8341 GAATAGCTCA GAGGCCGAGG CGGCCTCGGC CTCTGCATAA ATAAAAAAAA TTAGTCAGCC 
     8401 ATGGGGCGGA GAATGGGCGG AACTGGGCGG AGTTAGGGGC GGGATGGGCG GAGTTAGGGG 
     8461 CGGGACTATG GTTGCTGACT AATTGAGATG CATGCTTTGC ATACTTCTGC CTGCTGGGGA 
     8521 GCCTGGGGAC TTTCCACACC TGGTTGCTGA CTAATTGAGA TGCATGCTTT GCATACTTCT 
     8581 GCCTGCTGGG GAGCCTGGGG ACTTTCCACA CCCTAACTGA CACACATTCC ACAGCTGGTT 
     8641 CTTTCCGCCT CAGGACTCTT CCTTTTTCAA TATTATTGAA GCATTTATCA GGGTTATTGT 
     8701 CTCATGAGCG GATACATATT TGAATGTATT TAGAAAAATA AACAAATAGG GGTTCCGCGC 
     8761 ACATTTCCCC GAAAAGTGCC ACCTGACGCG CCCTGTAGCG GCGCATTAAG CGCGGCGGGT 
     8821 GTGGTGGTTA CGCGCAGCGT GACCGCTACA CTTGCCAGCG CCCTAGCGCC CGCTCCTTTC 
     8881 GCTTTCTTCC CTTCCTTTCT CGCCACGTTC GCCGGCTTTC CCCGTCAAGC TCTAAATCGG 
     8941 GGGCTCCCTT TAGGGTTCCG ATTTAGTGCT TTACGGCACC TCGACCCCAA AAAACTTGAT 
     9001 TAGGGTGATG GTTCACGTAG TGGGCCATCG CCCTGATAGA CGGTTTTTCG CCCTTTGACG 
     9061 TTGGAGTCCA CGTTCTTTAA TAGTGGACTC TTGTTCCAAA CTGGAACAAC ACTCAACCCT 
     9121 ATCTCGGTCT ATTCTTTTGA TTTATAAGGG ATTTTGCCGA TTTCGGCCTA TTGGTTAAAA 
     9181 AATGAGCTGA TTTAACAAAA ATTTAACGCG AATTTT
//