LOCUS       RC213335                9378 bp    DNA     circular  SYN 
DEFINITION  COL5A2 (Myc-DDK-tagged)-Human collagen, type V, alpha 2 (COL5A2)
ACCESSION   NM_000393
VERSION     NM_000393.3
SOURCE      synthetic construct 
FEATURES    Location/Qualifiers 
     misc_feature    201..924 
                     /label="CMV promoter"  
     misc_feature    839..856 
                     /label="Restriction Enzyme"  
     misc_feature    953..971 
                     /label="T7 promoter"  
     misc_feature    5535..5626 
                     /label="Myc-DDK"  
     misc_feature    5656..6241 
                     /label="PolyA signal"  
     misc_feature    5713..5732 
                     /label="Restriction Enzyme"  
     misc_feature    6517..7189 
                     /label="ColE1"  
     misc_feature    7619..8413 
                     /label="Kan/Neo"  
     misc_feature    8448..8750 
                     /label="SV40 ori"  
     misc_feature    8836..9313 
                     /label="f1 ori"  
     misc_feature    1020..1028 
                     /label="Restriction Enzyme"  
     misc_feature    5517..5523 
                     /label="Restriction Enzyme"  
     misc_feature    1020..5517 
                     /label="COL5A2"  
ORIGIN                      
        1 AACAAAATAT TAACGCTTAC AATTTCCATT CGCCATTCAG GCTGCGCAAC TGTTGGGAAG 
       61 GGCGATCGGT GCGGGCCTCT TCGCTATTAC GCCAGCTGGC GAAAGGGGGA TGTGCTGCAA 
      121 GGCGATTAAG TTGGGTAACG CCAGGGTTTT CCCAGTCACG ACGTTGTAAA ACGACGGCCA 
      181 GTGCCAAGCT GATCTATACA TTGAATCAAT ATTGGCAATT AGCCATATTA GTCATTGGTT 
      241 ATATAGCATA AATCAATATT GGCTATTGGC CATTGCATAC GTTGTATCTA TATCATAATA 
      301 TGTACATTTA TATTGGCTCA TGTCCAATAT GACCGCCATG TTGACATTGA TTATTGACTA 
      361 GTTATTAATA GTAATCAATT ACGGGGTCAT TAGTTCATAG CCCATATATG GAGTTCCGCG 
      421 TTACATAACT TACGGTAAAT GGCCCGCCTG GCTGACCGCC CAACGACCCC CGCCCATTGA 
      481 CGTCAATAAT GACGTATGTT CCCATAGTAA CGCCAATAGG GACTTTCCAT TGACGTCAAT 
      541 GGGTGGAGTA TTTACGGTAA ACTGCCCACT TGGCAGTACA TCAAGTGTAT CATATGCCAA 
      601 GTCCGCCCCC TATTGACGTC AATGACGGTA AATGGCCCGC CTGGCATTAT GCCCAGTACA 
      661 TGACCTTACG GGACTTTCCT ACTTGGCAGT ACATCTACGT ATTAGTCATC GCTATTACCA 
      721 TGGTGATGCG GTTTTGGCAG TACACCAATG GGCGTGGATA GCGGTTTGAC TCACGGGGAT 
      781 TTCCAAGTCT CCACCCCATT GACGTCAATG GGAGTTTGTT TTGGCACCAA AATCAACGGG 
      841 ACTTTCCAAA ATGTCGTAAT AACCCCGCCC CGTTGACGCA AATGGGCGGT AGGCGTGTAC 
      901 GGTGGGAGGT CTATATAAGC AGAGCTCGTT TAGTGAACCG TCAGAATTTT GTAATACGAC 
      961 TCACTATAGG GCGGCCGGGA ATTCGTCGAC TGGATCCGGT ACCGAGGAGA TCTGCCGCCG 
     1021 CGATCGCCAT GATGGCAAAC TGGGCGGAAG CAAGACCTCT CCTCATTCTT ATTGTTTTAT 
     1081 TAGGGCAATT TGTCTCAATA AAAGCCCAGG AAGAAGACGA GGATGAAGGA TATGGTGAAG 
     1141 AAATAGCCTG CACTCAGAAT GGCCAGATGT ACTTAAACAG GGACATTTGG AAACCTGCCC 
     1201 CTTGTCAGAT CTGTGTCTGT GACAATGGAG CCATTCTCTG TGACAAGATA GAATGCCAGG 
     1261 ATGTGCTGGA CTGTGCCGAC CCTGTAACGC CCCCTGGGGA ATGCTGTCCT GTCTGTTCAC 
     1321 AAACACCTGG AGGTGGCAAT ACAAATTTTG GTAGAGGAAG AAAGGGACAA AAGGGAGAAC 
     1381 CAGGATTAGT GCCTGTTGTA ACAGGCATAC GTGGTCGTCC AGGACCGGCA GGACCTCCAG 
     1441 GATCACAGGG ACCAAGAGGA GAGCGAGGGC CAAAAGGAAG ACCTGGCCCT CGTGGACCTC 
     1501 AGGGAATTGA TGGAGAACCA GGTGTTCCTG GTCAACCTGG TGCTCCAGGA CCTCCTGGAC 
     1561 ATCCGTCCCA CCCAGGACCC GATGGCTTGA GCAGGCCGTT TTCAGCTCAA ATGGCTGGGT 
     1621 TGGATGAAAA ATCTGGACTT GGGAGTCAAG TAGGACTAAT GCCTGGCTCT GTGGGTCCTG 
     1681 TTGGCCCAAG GGGACCACAG GGTTTACAAG GACAGCAAGG TGGTGCAGGA CCTACAGGAC 
     1741 CTCCTGGTGA ACCTGGTGAT CCTGGACCAA TGGGTCCGAT TGGTTCACGT GGACCAGAGG 
     1801 GCCCTCCTGG TAAACCTGGG GAAGATGGTG AACCTGGCAG AAATGGAAAT CCTGGTGAAG 
     1861 TGGGATTTGC AGGATCTCCG GGAGCTCGTG GATTTCCTGG GGCTCCTGGT CTTCCAGGTC 
     1921 TGAAGGGTCA CCGAGGACAC AAAGGTCTTG AAGGCCCTAA AGGTGAAGTT GGAGCACCTG 
     1981 GTTCCAAGGG TGAAGCTGGC CCCACTGGTC CAATGGGTGC CATGGGTCCT CTGGGTCCGA 
     2041 GGGGAATGCC AGGAGAGAGA GGGAGACTTG GGCCACAGGG TGCTCCTGGA CAACGAGGTG 
     2101 CACATGGTAT GCCTGGAAAA CCTGGACCAA TGGGTCCTCT TGGGATACCA GGCTCTTCTG 
     2161 GTTTTCCAGG AAATCCTGGA ATGAAGGGAG AAGCAGGTCC TACAGGGGCG CGAGGCCCTG 
     2221 AAGGTCCTCA GGGGCAGAGA GGTGAAACTG GGCCCCCAGG TCCAGTTGGC TCTCCAGGTC 
     2281 TTCCTGGTGC AATAGGAACT GATGGTACTC CTGGTGCCAA AGGCCCAACG GGCTCTCCGG 
     2341 GTACCTCTGG TCCTCCTGGC TCAGCAGGGC CTCCTGGATC TCCAGGACCT CAGGGTAGCA 
     2401 CTGGTCCTCA GGGAATTCGA GGCCAACCGG GTGATCCAGG AGTTCCAGGT TTCAAAGGAG 
     2461 AAGCTGGCCC AAAAGGGGAA CCAGGGCCAC ATGGTATTCA GGGTCCGATA GGCCCACCCG 
     2521 GTGAAGAAGG CAAAAGAGGT CCCAGAGGTG ACCCAGGAAC AGTTGGTCCT CCAGGGCCAG 
     2581 TGGGAGAAAG GGGTGCTCCT GGCAATCGTG GTTTTCCAGG CTCTGATGGT TTACCTGGGC 
     2641 CAAAGGGTGC TCAAGGAGAA CGGGGTCCTG TAGGTTCTTC AGGACCCAAA GGAAGCCAGG 
     2701 GGGATCCAGG ACGTCCAGGG GAACCTGGGC TTCCAGGTGC TCGGGGTTTG ACAGGAAATC 
     2761 CTGGTGTTCA AGGTCCTGAA GGAAAACTTG GACCTTTGGG TGCGCCAGGG GAAGATGGCC 
     2821 GTCCAGGTCC TCCAGGCTCC ATAGGAATCA GAGGGCAGCC CGGGAGCATG GGCCTTCCAG 
     2881 GCCCCAAAGG TAGCAGTGGT GACCCTGGGA AACCTGGAGA AGCAGGAAAT GCTGGAGTTC 
     2941 CTGGGCAGAG GGGAGCTCCT GGAAAAGATG GTGAAGTTGG TCCTTCTGGT CCTGTGGGCC 
     3001 CGCCGGGTCT AGCTGGTGAA AGAGGAGAAC AAGGACCTCC AGGCCCCACA GGTTTTCAGG 
     3061 GGCTTCCTGG TCCTCCAGGG CCTCCTGGAG AAGGTGGAAA ACCAGGTGAT CAAGGTGTTC 
     3121 CTGGAGATCC CGGAGCAGTT GGCCCGTTAG GACCTAGAGG AGAACGAGGA AATCCTGGGG 
     3181 AAAGAGGAGA ACCTGGGATA ACTGGACTCC CTGGTGAGAA GGGAATGGCT GGAGGACATG 
     3241 GTCCTGATGG CCCAAAAGGC AGTCCAGGTC CATCTGGGAC CCCTGGAGAT ACAGGCCCAC 
     3301 CAGGTCTTCA AGGTATGCCG GGAGAAAGAG GAATTGCAGG AACTCCTGGC CCCAAGGGTG 
     3361 ACAGAGGTGG CATAGGAGAA AAAGGTGCTG AAGGCACAGC TGGAAATGAT GGTGCAAGAG 
     3421 GTCTTCCAGG TCCTTTGGGC CCTCCAGGTC CGGCAGGTCC TACTGGAGAA AAGGGTGAAC 
     3481 CTGGTCCTCG AGGTTTAGTT GGCCCTCCTG GCTCCCGGGG CAATCCTGGT TCTCGAGGTG 
     3541 AAAATGGGCC AACTGGAGCT GTTGGTTTTG CCGGACCCCA GGGTCCTGAC GGACAGCCTG 
     3601 GAGTAAAAGG TGAACCTGGA GAGCCAGGAC AGAAGGGAGA TGCTGGTTCT CCTGGACCAC 
     3661 AAGGTTTAGC AGGATCCCCT GGCCCTCATG GTCCTAATGG TGTTCCTGGA CTAAAAGGTG 
     3721 GTCGAGGAAC CCAAGGTCCG CCTGGTGCTA CAGGATTTCC TGGTTCTGCG GGCAGAGTTG 
     3781 GACCTCCAGG CCCTGCTGGA GCTCCAGGAC CTGCGGGACC CCTAGGGGAA CCCGGGAAGG 
     3841 AGGGACCTCC AGGTCTTCGT GGGGACCCTG GCTCTCATGG GCGTGTGGGA GATCGAGGAC 
     3901 CAGCTGGCCC CCCTGGTGGC CCAGGAGACA AAGGGGACCC AGGAGAAGAT GGGCAACCTG 
     3961 GTCCAGATGG CCCCCCTGGT CCAGCTGGAA CGACCGGGCA GAGAGGAATT GTTGGCATGC 
     4021 CTGGGCAACG TGGAGAGAGA GGCATGCCCG GCCTACCAGG CCCAGCGGGA ACACCAGGAA 
     4081 AAGTAGGACC AACTGGTGCA ACAGGAGATA AAGGTCCACC TGGACCTGTG GGGCCCCCAG 
     4141 GCTCCAATGG TCCTGTAGGG GAACCTGGAC CAGAAGGTCC AGCTGGCAAT GATGGTACCC 
     4201 CAGGACGGGA TGGTGCTGTT GGAGAACGTG GTGATCGTGG AGACCCTGGG CCTGCAGGTC 
     4261 TGCCAGGCTC TCAGGGTGCC CCTGGAACTC CTGGCCCTGT GGGTGCTCCA GGAGATGCAG 
     4321 GACAAAGAGG AGATCCGGGT TCTCGGGGTC CTATAGGACC ACCTGGTCGA GCTGGGAAAC 
     4381 GTGGATTACC TGGACCCCAA GGACCTCGTG GTGACAAAGG TGATCATGGA GACCGAGGCG 
     4441 ACAGAGGTCA GAAGGGCCAC AGAGGCTTTA CTGGTCTTCA GGGTCTTCCT GGCCCTCCTG 
     4501 GTCCAAATGG TGAACAAGGA AGTGCTGGAA TCCCTGGACC ATTTGGCCCA AGAGGTCCTC 
     4561 CAGGCCCAGT TGGTCCTTCA GGTAAAGAAG GAAACCCTGG GCCACTTGGG CCAATTGGAC 
     4621 CTCCAGGTGT ACGAGGCAGT GTAGGAGAAG CAGGACCTGA GGGCCCTCCT GGTGAGCCTG 
     4681 GCCCACCTGG CCCTCCGGGT CCCCCTGGCC ACCTTACAGC TGCTCTTGGG GATATCATGG 
     4741 GGCACTATGA TGAAAGCATG CCAGATCCAC TTCCTGAGTT TACTGAAGAT CAGGCGGCTC 
     4801 CTGATGACAA AAACAAAACG GACCCAGGGG TTCATGCTAC CCTGAAGTCA CTCAGTAGTC 
     4861 AGATTGAAAC CATGCGCAGC CCCGATGGCT CGAAAAAGCA CCCAGCCCGC ACGTGTGATG 
     4921 ACCTAAAGCT TTGCCATTCC GCAAAGCAGA GTGGTGAATA CTGGATTGAT CCTAACCAAG 
     4981 GATCTGTTGA AGATGCAATC AAAGTTTACT GCAACATGGA AACAGGAGAA ACATGTATTT 
     5041 CAGCAAACCC ATCCAGTGTA CCACGTAAAA CCTGGTGGGC CAGTAAATCT CCTGACAATA 
     5101 AACCTGTTTG GTATGGTCTT GATATGAACA GAGGGTCTCA GTTCGCTTAT GGAGACCACC 
     5161 AATCACCTAA TACAGCCATT ACTCAGATGA CTTTTTTGCG CCTTTTATCA AAAGAAGCCT 
     5221 CCCAGAACAT CACTTACATC TGTAAAAACA GTGTAGGATA CATGGACGAT CAAGCTAAGA 
     5281 ACCTCAAAAA AGCTGTGGTT CTCAAAGGGG CAAATGACTT AGATATCAAA GCAGAGGGAA 
     5341 ATATTAGATT CCGGTATATC GTTCTTCAAG ACACTTGCTC TAAGCGGAAT GGAAATGTGG 
     5401 GCAAGACTGT CTTTGAATAT AGAACACAGA ATGTGGCACG CTTGCCCATC ATAGATCTTG 
     5461 CTCCTGTGGA TGTTGGCGGC ACAGACCAGG AATTCGGCGT TGAAATTGGG CCAGTTTGTT 
     5521 TTGTGACGCG TACGCGGCCG CTCGAGCAGA AACTCATCTC AGAAGAGGAT CTGGCAGCAA 
     5581 ATGATATCCT GGATTACAAG GATGACGACG ATAAGGTTTA AACGGCCGGC CGCGGTCATA 
     5641 GCTGTTTCCT GAACAGATCC CGGGTGGCAT CCCTGTGACC CCTCCCCAGT GCCTCTCCTG 
     5701 GCCCTGGAAG TTGCCACTCC AGTGCCCACC AGCCTTGTCC TAATAAAATT AAGTTGCATC 
     5761 ATTTTGTCTG ACTAGGTGTC CTTCTATAAT ATTATGGGGT GGAGGGGGGT GGTATGGAGC 
     5821 AAGGGGCAAG TTGGGAAGAC AACCTGTAGG GCCTGCGGGG TCTATTGGGA ACCAAGCTGG 
     5881 AGTGCAGTGG CACAATCTTG GCTCACTGCA ATCTCCGCCT CCTGGGTTCA AGCGATTCTC 
     5941 CTGCCTCAGC CTCCCGAGTT GTTGGGATTC CAGGCATGCA TGACCAGGCT CAGCTAATTT 
     6001 TTGTTTTTTT GGTAGAGACG GGGTTTCACC ATATTGGCCA GGCTGGTCTC CAACTCCTAA 
     6061 TCTCAGGTGA TCTACCCACC TTGGCCTCCC AAATTGCTGG GATTACAGGC GTGAACCACT 
     6121 GCTCCCTTCC CTGTCCTTCT GATTTTAAAA TAACTATACC AGCAGGAGGA CGTCCAGACA 
     6181 CAGCATAGGC TACCTGGCCA TGCCCAACCG GTGGGACATT TGAGTTGCTT GCTTGGCACT 
     6241 GTCCTCTCAT GCGTTGGGTC CACTCAGTAG ATGCCTGTTG AATTGGGTAC GCGGCCAGCG 
     6301 GCGAGCGGTA TCAGCTCACT CAAAGGCGGT AATACGGTTA TCCACAGAAT CAGGGGATAA 
     6361 CGCAGGAAAG AACATGTGAG CAAAAGGCCA GCAAAAGGCC AGGAACCGTA AAAAGGCCGC 
     6421 GTTGCTGGCG TTTTTCCATA GGCTCCGCCC CCCTGACGAG CATCACAAAA ATCGACGCTC 
     6481 AAGTCAGAGG TGGCGAAACC CGACAGGACT ATAAAGATAC CAGGCGTTTC CCCCTGGAAG 
     6541 CTCCCTCGTG CGCTCTCCTG TTCCGACCCT GCCGCTTACC GGATACCTGT CCGCCTTTCT 
     6601 CCCTTCGGGA AGCGTGGCGC TTTCTCATAG CTCACGCTGT AGGTATCTCA GTTCGGTGTA 
     6661 GGTCGTTCGC TCCAAGCTGG GCTGTGTGCA CGAACCCCCC GTTCAGCCCG ACCGCTGCGC 
     6721 CTTATCCGGT AACTATCGTC TTGAGTCCAA CCCGGTAAGA CACGACTTAT CGCCACTGGC 
     6781 AGCAGCCACT GGTAACAGGA TTAGCAGAGC GAGGTATGTA GGCGGTGCTA CAGAGTTCTT 
     6841 GAAGTGGTGG CCTAACTACG GCTACACTAG AAGAACAGTA TTTGGTATCT GCGCTCTGCT 
     6901 GAAGCCAGTT ACCTTCGGAA AAAGAGTTGG TAGCTCTTGA TCCGGCAAAC AAACCACCGC 
     6961 TGGTAGCGGT GGTTTTTTTG TTTGCAAGCA GCAGATTACG CGCAGAAAAA AAGGATCTCA 
     7021 AGAAGATCCT TTGATCTTTT CTACGGGGTC TGACGCTCAG TGGAACGAAA ACTCACGTTA 
     7081 AGGGATTTTG GTCATGAGAT TATCAAAAAG GATCTTCACC TAGATCCTTT TAAATTAAAA 
     7141 ATGAAGTTTT AAATCAATCT AAAGTATATA TGAGTAACCT GAGGCTATGG CAGGGCCTGC 
     7201 CGCCCCGACG TTGGCTGCGA GCCCTGGGCC TTCACCCGAA CTTGGGGGGT GGGGTGGGGA 
     7261 AAAGGAAGAA ACGCGGGCGT ATTGGCCCCA ATGGGGTCTC GGTGGGGTAT CGACAGAGTG 
     7321 CCAGCCCTGG GACCGAACCC CGCGTTTATG AACAAACGAC CCAACACCGT GCGTTTTATT 
     7381 CTGTCTTTTT ATTGCCGTCA TAGCGCGGGT TCCTTCCGGT ATTGTCTCCT TCCGTGTTTC 
     7441 AGTTAGCCTC CCCCTAGGGT GGGCGAAGAA CTCCAGCATG AGATCCCCGC GCTGGAGGAT 
     7501 CATCCAGCCG GCGTCCCGGA AAACGATTCC GAAGCCCAAC CTTTCATAGA AGGCGGCGGT 
     7561 GGAATCGAAA TCTCGTGATG GCAGGTTGGG CGTCGCTTGG TCGGTCATTT CGAACCCCAG 
     7621 AGTCCCGCTC AGAAGAACTC GTCAAGAAGG CGATAGAAGG CGATGCGCTG CGAATCGGGA 
     7681 GCGGCGATAC CGTAAAGCAC GAGGAAGCGG TCAGCCCATT CGCCGCCAAG CTCTTCAGCA 
     7741 ATATCACGGG TAGCCAACGC TATGTCCTGA TAGCGATCCG CCACACCCAG CCGGCCACAG 
     7801 TCGATGAATC CAGAAAAGCG GCCATTTTCC ACCATGATAT TCGGCAAGCA GGCATCGCCA 
     7861 TGGGTCACGA CGAGATCCTC GCCGTCGGGC ATGCTCGCCT TGAGCCTGGC GAACAGTTCG 
     7921 GCTGGCGCGA GCCCCTGATG CTCTTCGTCC AGATCATCCT GATCGACAAG ACCGGCTTCC 
     7981 ATCCGAGTAC GTGCTCGCTC GATGCGATGT TTCGCTTGGT GGTCGAATGG GCAGGTAGCC 
     8041 GGATCAAGCG TATGCAGCCG CCGCATTGCA TCAGCCATGA TGGATACTTT CTCGGCAGGA 
     8101 GCAAGGTGAG ATGACAGGAG ATCCTGCCCC GGCACTTCGC CCAATAGCAG CCAGTCCCTT 
     8161 CCCGCTTCAG TGACAACGTC GAGCACAGCT GCGCAAGGAA CGCCCGTCGT GGCCAGCCAC 
     8221 GATAGCCGCG CTGCCTCGTC TTGCAGTTCA TTCAGGGCAC CGGACAGGTC GGTCTTGACA 
     8281 AAAAGAACCG GGCGCCCCTG CGCTGACAGC CGGAACACGG CGGCATCAGA GCAGCCGATT 
     8341 GTCTGTTGTG CCCAGTCATA GCCGAATAGC CTCTCCACCC AAGCGGCCGG AGAACCTGCG 
     8401 TGCAATCCAT CTTGTTCAAT CATGCGAAAC GATCCTCATC CTGTCTCTTG ATCGATCTTT 
     8461 GCAAAAGCCT AGGCCTCCAA AAAAGCCTCC TCACTACTTC TGGAATAGCT CAGAGGCCGA 
     8521 GGCGGCCTCG GCCTCTGCAT AAATAAAAAA AATTAGTCAG CCATGGGGCG GAGAATGGGC 
     8581 GGAACTGGGC GGAGTTAGGG GCGGGATGGG CGGAGTTAGG GGCGGGACTA TGGTTGCTGA 
     8641 CTAATTGAGA TGCATGCTTT GCATACTTCT GCCTGCTGGG GAGCCTGGGG ACTTTCCACA 
     8701 CCTGGTTGCT GACTAATTGA GATGCATGCT TTGCATACTT CTGCCTGCTG GGGAGCCTGG 
     8761 GGACTTTCCA CACCCTAACT GACACACATT CCACAGCTGG TTCTTTCCGC CTCAGGACTC 
     8821 TTCCTTTTTC AATATTATTG AAGCATTTAT CAGGGTTATT GTCTCATGAG CGGATACATA 
     8881 TTTGAATGTA TTTAGAAAAA TAAACAAATA GGGGTTCCGC GCACATTTCC CCGAAAAGTG 
     8941 CCACCTGACG CGCCCTGTAG CGGCGCATTA AGCGCGGCGG GTGTGGTGGT TACGCGCAGC 
     9001 GTGACCGCTA CACTTGCCAG CGCCCTAGCG CCCGCTCCTT TCGCTTTCTT CCCTTCCTTT 
     9061 CTCGCCACGT TCGCCGGCTT TCCCCGTCAA GCTCTAAATC GGGGGCTCCC TTTAGGGTTC 
     9121 CGATTTAGTG CTTTACGGCA CCTCGACCCC AAAAAACTTG ATTAGGGTGA TGGTTCACGT 
     9181 AGTGGGCCAT CGCCCTGATA GACGGTTTTT CGCCCTTTGA CGTTGGAGTC CACGTTCTTT 
     9241 AATAGTGGAC TCTTGTTCCA AACTGGAACA ACACTCAACC CTATCTCGGT CTATTCTTTT 
     9301 GATTTATAAG GGATTTTGCC GATTTCGGCC TATTGGTTAA AAAATGAGCT GATTTAACAA 
     9361 AAATTTAACG CGAATTTT
//