LOCUS RC213332 8385 bp DNA circular SYN DEFINITION TSC1 (Myc-DDK-tagged)-Human tuberous sclerosis 1 (TSC1), transcript variant 1 ACCESSION NM_000368 VERSION null SOURCE synthetic construct FEATURES Location/Qualifiers misc_feature 201..924 /label="CMV promoter" misc_feature 839..856 /label="Restriction Enzyme" misc_feature 953..971 /label="T7 promoter" misc_feature 4542..4633 /label="Myc-DDK" misc_feature 4663..5248 /label="PolyA signal" misc_feature 4720..4739 /label="Restriction Enzyme" misc_feature 5524..6196 /label="ColE1" misc_feature 6626..7420 /label="Kan/Neo" misc_feature 7455..7757 /label="SV40 ori" misc_feature 7843..8320 /label="f1 ori" misc_feature 1020..1028 /label="Restriction Enzyme" misc_feature 4524..4530 /label="Restriction Enzyme" misc_feature 1020..4524 /label="TSC1" ORIGIN 1 AACAAAATAT TAACGCTTAC AATTTCCATT CGCCATTCAG GCTGCGCAAC TGTTGGGAAG 61 GGCGATCGGT GCGGGCCTCT TCGCTATTAC GCCAGCTGGC GAAAGGGGGA TGTGCTGCAA 121 GGCGATTAAG TTGGGTAACG CCAGGGTTTT CCCAGTCACG ACGTTGTAAA ACGACGGCCA 181 GTGCCAAGCT GATCTATACA TTGAATCAAT ATTGGCAATT AGCCATATTA GTCATTGGTT 241 ATATAGCATA AATCAATATT GGCTATTGGC CATTGCATAC GTTGTATCTA TATCATAATA 301 TGTACATTTA TATTGGCTCA TGTCCAATAT GACCGCCATG TTGACATTGA TTATTGACTA 361 GTTATTAATA GTAATCAATT ACGGGGTCAT TAGTTCATAG CCCATATATG GAGTTCCGCG 421 TTACATAACT TACGGTAAAT GGCCCGCCTG GCTGACCGCC CAACGACCCC CGCCCATTGA 481 CGTCAATAAT GACGTATGTT CCCATAGTAA CGCCAATAGG GACTTTCCAT TGACGTCAAT 541 GGGTGGAGTA TTTACGGTAA ACTGCCCACT TGGCAGTACA TCAAGTGTAT CATATGCCAA 601 GTCCGCCCCC TATTGACGTC AATGACGGTA AATGGCCCGC CTGGCATTAT GCCCAGTACA 661 TGACCTTACG GGACTTTCCT ACTTGGCAGT ACATCTACGT ATTAGTCATC GCTATTACCA 721 TGGTGATGCG GTTTTGGCAG TACACCAATG GGCGTGGATA GCGGTTTGAC TCACGGGGAT 781 TTCCAAGTCT CCACCCCATT GACGTCAATG GGAGTTTGTT TTGGCACCAA AATCAACGGG 841 ACTTTCCAAA ATGTCGTAAT AACCCCGCCC CGTTGACGCA AATGGGCGGT AGGCGTGTAC 901 GGTGGGAGGT CTATATAAGC AGAGCTCGTT TAGTGAACCG TCAGAATTTT GTAATACGAC 961 TCACTATAGG GCGGCCGGGA ATTCGTCGAC TGGATCCGGT ACCGAGGAGA TCTGCCGCCG 1021 CGATCGCCAT GGCCCAACAA GCAAATGTCG GGGAGCTTCT TGCCATGCTG GACTCCCCCA 1081 TGCTGGGTGT GCGGGACGAC GTGACAGCTG TCTTTAAAGA GAACCTCAAT TCTGACCGTG 1141 GCCCTATGCT TGTAAACACC TTGGTGGATT ATTACCTGGA AACCAGCTCT CAGCCGGCAT 1201 TGCACATCCT GACCACCTTG CAAGAGCCAC ATGACAAGCA CCTCTTGGAC AGGATTAACG 1261 AATATGTGGG CAAAGCCGCC ACTCGTTTAT CCATCCTCTC GTTACTGGGT CATGTCATAA 1321 GACTGCAGCC ATCTTGGAAG CATAAGCTCT CTCAAGCACC TCTTTTGCCT TCTTTACTAA 1381 AATGTCTCAA GATGGACACT GACGTCGTTG TCCTCACAAC AGGCGTCTTG GTGTTGATAA 1441 CCATGCTACC AATGATTCCA CAGTCTGGGA AACAGCATCT TCTTGATTTC TTTGACATTT 1501 TTGGCCGTCT GTCATCATGG TGCCTGAAGA AACCAGGCCA CGTGGCGGAA GTCTATCTCG 1561 TCCATCTCCA TGCCAGTGTG TACGCACTCT TTCATCGCCT TTATGGAATG TACCCTTGCA 1621 ACTTCGTCTC CTTTTTGCGT TCTCATTACA GTATGAAAGA AAACCTGGAG ACTTTTGAAG 1681 AAGTGGTCAA GCCAATGATG GAGCATGTGC GAATTCATCC GGAATTAGTG ACTGGATCCA 1741 AGGACCATGA ACTGGACCCT CGAAGGTGGA AGAGATTAGA AACTCATGAT GTTGTGATCG 1801 AGTGTGCCAA AATCTCTCTG GATCCCACAG AAGCCTCATA TGAAGATGGC TATTCTGTGT 1861 CTCACCAAAT CTCAGCCCGC TTTCCTCATC GTTCAGCCGA TGTCACCACC AGCCCTTATG 1921 CTGACACACA GAATAGCTAT GGGTGTGCTA CTTCTACCCC TTACTCCACG TCTCGGCTGA 1981 TGTTGTTAAA TATGCCAGGG CAGCTACCTC AGACTCTGAG TTCCCCATCG ACACGGCTGA 2041 TAACTGAACC ACCACAAGCT ACTCTTTGGA GCCCATCTAT GGTTTGTGGT ATGACCACTC 2101 CTCCAACTTC TCCTGGAAAT GTCCCACCTG ATCTGTCACA CCCTTACAGT AAAGTCTTTG 2161 GTACAACTGC AGGTGGAAAA GGAACTCCTC TGGGAACCCC AGCAACCTCT CCTCCTCCAG 2221 CCCCACTCTG TCATTCGGAT GACTACGTGC ACATTTCACT CCCCCAGGCC ACAGTCACAC 2281 CCCCCAGGAA GGAAGAGAGA ATGGATTCTG CAAGACCATG TCTACACAGA CAACACCATC 2341 TTCTGAATGA CAGAGGATCA GAAGAGCCAC CTGGCAGCAA AGGTTCTGTC ACTCTAAGTG 2401 ATCTTCCAGG GTTTTTAGGT GATCTGGCCT CTGAAGAAGA TAGTATTGAA AAAGATAAAG 2461 AAGAAGCTGC AATATCTAGA GAACTTTCTG AGATCACCAC AGCAGAGGCA GAGCCTGTGG 2521 TTCCTCGAGG AGGCTTTGAC TCTCCCTTTT ACCGAGACAG TCTCCCAGGT TCTCAGCGGA 2581 AGACCCACTC GGCAGCCTCC AGTTCTCAGG GCGCCAGCGT GAACCCTGAG CCTTTACACT 2641 CCTCCCTGGA CAAGCTTGGG CCTGACACAC CAAAGCAAGC CTTTACTCCC ATAGACCTGC 2701 CCTGCGGCAG TGCTGATGAA AGCCCTGCGG GAGACAGGGA ATGCCAGACT TCTTTGGAGA 2761 CCAGTATCTT CACTCCCAGT CCTTGTAAAA TTCCACCTCC GACGAGAGTG GGCTTTGGAA 2821 GCGGGCAGCC TCCCCCGTAT GATCATCTTT TTGAGGTGGC ATTGCCAAAG ACAGCCCATC 2881 ATTTTGTCAT CAGGAAGACT GAGGAGCTGT TAAAGAAAGC AAAAGGAAAC ACAGAGGAAG 2941 ATGGTGTGCC CTCTACCTCC CCAATGGAAG TGCTGGACAG ACTGATACAG CAGGGAGCAG 3001 ACGCGCACAG CAAGGAGCTG AACAAGTTGC CTTTACCCAG CAAGTCTGTC GACTGGACCC 3061 ACTTTGGAGG CTCTCCTCCT TCAGATGAGA TCCGCACCCT CCGAGACCAG TTGCTTTTAC 3121 TGCACAACCA GTTACTCTAT GAGCGTTTTA AGAGGCAGCA GCATGCCCTC CGGAACAGGC 3181 GGCTCCTCCG CAAGGTGATC AAAGCAGCAG CTCTGGAGGA ACATAATGCT GCCATGAAAG 3241 ATCAGTTGAA GTTACAAGAG AAGGACATCC AGATGTGGAA GGTTAGTCTG CAGAAAGAAC 3301 AAGCTAGATA CAATCAGCTC CAGGAGCAGC GTGACACTAT GGTAACCAAG CTCCACAGCC 3361 AGATCAGACA GCTGCAGCAT GACCGAGAGG AATTCTACAA CCAGAGCCAG GAATTACAGA 3421 CGAAGCTGGA GGACTGCAGG AACATGATTG CGGAGCTGCG GATAGAACTG AAGAAGGCCA 3481 ACAACAAGGT GTGTCACACT GAGCTGCTGC TCAGTCAGGT TTCCCAAAAG CTCTCAAACA 3541 GTGAGTCGGT CCAGCAGCAG ATGGAGTTCT TGAACAGGCA GCTGTTGGTT CTTGGGGAGG 3601 TCAACGAGCT CTATTTGGAA CAACTGCAGA ACAAGCACTC AGATACCACA AAGGAAGTAG 3661 AAATGATGAA AGCCGCCTAT CGGAAAGAGC TAGAAAAAAA CAGAAGCCAT GTTCTCCAGC 3721 AGACTCAGAG GCTTGATACC TCCCAAAAAC GGATTTTGGA ACTGGAATCT CACCTGGCCA 3781 AGAAAGACCA CCTTCTTTTG GAACAGAAGA AATATCTAGA GGATGTCAAA CTCCAGGCAA 3841 GAGGACAGCT GCAGGCCGCA GAGAGCAGGT ATGAGGCTCA GAAAAGGATA ACCCAGGTGT 3901 TTGAATTGGA GATCTTAGAT TTATATGGCA GGTTGGAGAA AGATGGCCTC CTGAAAAAAC 3961 TTGAAGAAGA AAAAGCAGAA GCAGCTGAAG CAGCAGAAGA AAGGCTTTCT TACAGGCTTG 4021 ACTGTTGTAA TGACGGGTGC TCAGATTCCA TGGTAGGGCA CAATGAAGAG GCATCTGGCC 4081 ACAACGGTGA GACCAAGACC CCCAGGCCCA GCAGCGCCCG GGGCAGTAGT GGAAGCAGAG 4141 GTGGTGGAGG CAGCAGCAGC AGCAGCAGCG AGCTTTCTAC CCCAGAGAAA CCCCCACACC 4201 AGAGGGCAGG CCCATTCAGC AGTCGGTGGG AGACGACTAT GGGAGAAGCG TCTGCCAGCA 4261 TCCCCACCAC TGTGGGCTCA CTTCCCAGTT CAAAAAGCTT CCTGGGTATG AAGGCTCGAG 4321 AGTTATTTCG TAATAAGAGC GAGAGCCAGT GTGATGAGGA CGGCATGACC AGTAGCCTTT 4381 CTGAGAGCCT AAAGACAGAA CTGGGCAAAG ACTTGGGTGT GGAAGCCAAG ATTCCCCTGA 4441 ACCTAGATGG CCCTCACCCG TCTCCCCCGA CCCCGGACAG TGTTGGACAG CTACATATCA 4501 TGGACTACAA TGAGACTCAT CATGAACACA GCACGCGTAC GCGGCCGCTC GAGCAGAAAC 4561 TCATCTCAGA AGAGGATCTG GCAGCAAATG ATATCCTGGA TTACAAGGAT GACGACGATA 4621 AGGTTTAAAC GGCCGGCCGC GGTCATAGCT GTTTCCTGAA CAGATCCCGG GTGGCATCCC 4681 TGTGACCCCT CCCCAGTGCC TCTCCTGGCC CTGGAAGTTG CCACTCCAGT GCCCACCAGC 4741 CTTGTCCTAA TAAAATTAAG TTGCATCATT TTGTCTGACT AGGTGTCCTT CTATAATATT 4801 ATGGGGTGGA GGGGGGTGGT ATGGAGCAAG GGGCAAGTTG GGAAGACAAC CTGTAGGGCC 4861 TGCGGGGTCT ATTGGGAACC AAGCTGGAGT GCAGTGGCAC AATCTTGGCT CACTGCAATC 4921 TCCGCCTCCT GGGTTCAAGC GATTCTCCTG CCTCAGCCTC CCGAGTTGTT GGGATTCCAG 4981 GCATGCATGA CCAGGCTCAG CTAATTTTTG TTTTTTTGGT AGAGACGGGG TTTCACCATA 5041 TTGGCCAGGC TGGTCTCCAA CTCCTAATCT CAGGTGATCT ACCCACCTTG GCCTCCCAAA 5101 TTGCTGGGAT TACAGGCGTG AACCACTGCT CCCTTCCCTG TCCTTCTGAT TTTAAAATAA 5161 CTATACCAGC AGGAGGACGT CCAGACACAG CATAGGCTAC CTGGCCATGC CCAACCGGTG 5221 GGACATTTGA GTTGCTTGCT TGGCACTGTC CTCTCATGCG TTGGGTCCAC TCAGTAGATG 5281 CCTGTTGAAT TGGGTACGCG GCCAGCGGCG AGCGGTATCA GCTCACTCAA AGGCGGTAAT 5341 ACGGTTATCC ACAGAATCAG GGGATAACGC AGGAAAGAAC ATGTGAGCAA AAGGCCAGCA 5401 AAAGGCCAGG AACCGTAAAA AGGCCGCGTT GCTGGCGTTT TTCCATAGGC TCCGCCCCCC 5461 TGACGAGCAT CACAAAAATC GACGCTCAAG TCAGAGGTGG CGAAACCCGA CAGGACTATA 5521 AAGATACCAG GCGTTTCCCC CTGGAAGCTC CCTCGTGCGC TCTCCTGTTC CGACCCTGCC 5581 GCTTACCGGA TACCTGTCCG CCTTTCTCCC TTCGGGAAGC GTGGCGCTTT CTCATAGCTC 5641 ACGCTGTAGG TATCTCAGTT CGGTGTAGGT CGTTCGCTCC AAGCTGGGCT GTGTGCACGA 5701 ACCCCCCGTT CAGCCCGACC GCTGCGCCTT ATCCGGTAAC TATCGTCTTG AGTCCAACCC 5761 GGTAAGACAC GACTTATCGC CACTGGCAGC AGCCACTGGT AACAGGATTA GCAGAGCGAG 5821 GTATGTAGGC GGTGCTACAG AGTTCTTGAA GTGGTGGCCT AACTACGGCT ACACTAGAAG 5881 AACAGTATTT GGTATCTGCG CTCTGCTGAA GCCAGTTACC TTCGGAAAAA GAGTTGGTAG 5941 CTCTTGATCC GGCAAACAAA CCACCGCTGG TAGCGGTGGT TTTTTTGTTT GCAAGCAGCA 6001 GATTACGCGC AGAAAAAAAG GATCTCAAGA AGATCCTTTG ATCTTTTCTA CGGGGTCTGA 6061 CGCTCAGTGG AACGAAAACT CACGTTAAGG GATTTTGGTC ATGAGATTAT CAAAAAGGAT 6121 CTTCACCTAG ATCCTTTTAA ATTAAAAATG AAGTTTTAAA TCAATCTAAA GTATATATGA 6181 GTAACCTGAG GCTATGGCAG GGCCTGCCGC CCCGACGTTG GCTGCGAGCC CTGGGCCTTC 6241 ACCCGAACTT GGGGGGTGGG GTGGGGAAAA GGAAGAAACG CGGGCGTATT GGCCCCAATG 6301 GGGTCTCGGT GGGGTATCGA CAGAGTGCCA GCCCTGGGAC CGAACCCCGC GTTTATGAAC 6361 AAACGACCCA ACACCGTGCG TTTTATTCTG TCTTTTTATT GCCGTCATAG CGCGGGTTCC 6421 TTCCGGTATT GTCTCCTTCC GTGTTTCAGT TAGCCTCCCC CTAGGGTGGG CGAAGAACTC 6481 CAGCATGAGA TCCCCGCGCT GGAGGATCAT CCAGCCGGCG TCCCGGAAAA CGATTCCGAA 6541 GCCCAACCTT TCATAGAAGG CGGCGGTGGA ATCGAAATCT CGTGATGGCA GGTTGGGCGT 6601 CGCTTGGTCG GTCATTTCGA ACCCCAGAGT CCCGCTCAGA AGAACTCGTC AAGAAGGCGA 6661 TAGAAGGCGA TGCGCTGCGA ATCGGGAGCG GCGATACCGT AAAGCACGAG GAAGCGGTCA 6721 GCCCATTCGC CGCCAAGCTC TTCAGCAATA TCACGGGTAG CCAACGCTAT GTCCTGATAG 6781 CGATCCGCCA CACCCAGCCG GCCACAGTCG ATGAATCCAG AAAAGCGGCC ATTTTCCACC 6841 ATGATATTCG GCAAGCAGGC ATCGCCATGG GTCACGACGA GATCCTCGCC GTCGGGCATG 6901 CTCGCCTTGA GCCTGGCGAA CAGTTCGGCT GGCGCGAGCC CCTGATGCTC TTCGTCCAGA 6961 TCATCCTGAT CGACAAGACC GGCTTCCATC CGAGTACGTG CTCGCTCGAT GCGATGTTTC 7021 GCTTGGTGGT CGAATGGGCA GGTAGCCGGA TCAAGCGTAT GCAGCCGCCG CATTGCATCA 7081 GCCATGATGG ATACTTTCTC GGCAGGAGCA AGGTGAGATG ACAGGAGATC CTGCCCCGGC 7141 ACTTCGCCCA ATAGCAGCCA GTCCCTTCCC GCTTCAGTGA CAACGTCGAG CACAGCTGCG 7201 CAAGGAACGC CCGTCGTGGC CAGCCACGAT AGCCGCGCTG CCTCGTCTTG CAGTTCATTC 7261 AGGGCACCGG ACAGGTCGGT CTTGACAAAA AGAACCGGGC GCCCCTGCGC TGACAGCCGG 7321 AACACGGCGG CATCAGAGCA GCCGATTGTC TGTTGTGCCC AGTCATAGCC GAATAGCCTC 7381 TCCACCCAAG CGGCCGGAGA ACCTGCGTGC AATCCATCTT GTTCAATCAT GCGAAACGAT 7441 CCTCATCCTG TCTCTTGATC GATCTTTGCA AAAGCCTAGG CCTCCAAAAA AGCCTCCTCA 7501 CTACTTCTGG AATAGCTCAG AGGCCGAGGC GGCCTCGGCC TCTGCATAAA TAAAAAAAAT 7561 TAGTCAGCCA TGGGGCGGAG AATGGGCGGA ACTGGGCGGA GTTAGGGGCG GGATGGGCGG 7621 AGTTAGGGGC GGGACTATGG TTGCTGACTA ATTGAGATGC ATGCTTTGCA TACTTCTGCC 7681 TGCTGGGGAG CCTGGGGACT TTCCACACCT GGTTGCTGAC TAATTGAGAT GCATGCTTTG 7741 CATACTTCTG CCTGCTGGGG AGCCTGGGGA CTTTCCACAC CCTAACTGAC ACACATTCCA 7801 CAGCTGGTTC TTTCCGCCTC AGGACTCTTC CTTTTTCAAT ATTATTGAAG CATTTATCAG 7861 GGTTATTGTC TCATGAGCGG ATACATATTT GAATGTATTT AGAAAAATAA ACAAATAGGG 7921 GTTCCGCGCA CATTTCCCCG AAAAGTGCCA CCTGACGCGC CCTGTAGCGG CGCATTAAGC 7981 GCGGCGGGTG TGGTGGTTAC GCGCAGCGTG ACCGCTACAC TTGCCAGCGC CCTAGCGCCC 8041 GCTCCTTTCG CTTTCTTCCC TTCCTTTCTC GCCACGTTCG CCGGCTTTCC CCGTCAAGCT 8101 CTAAATCGGG GGCTCCCTTT AGGGTTCCGA TTTAGTGCTT TACGGCACCT CGACCCCAAA 8161 AAACTTGATT AGGGTGATGG TTCACGTAGT GGGCCATCGC CCTGATAGAC GGTTTTTCGC 8221 CCTTTGACGT TGGAGTCCAC GTTCTTTAAT AGTGGACTCT TGTTCCAAAC TGGAACAACA 8281 CTCAACCCTA TCTCGGTCTA TTCTTTTGAT TTATAAGGGA TTTTGCCGAT TTCGGCCTAT 8341 TGGTTAAAAA ATGAGCTGAT TTAACAAAAA TTTAACGCGA ATTTT //