LOCUS       RC213320                8154 bp    DNA     circular  SYN 
DEFINITION  DLC1 (Myc-DDK-tagged)-Human deleted in liver cancer 1 (DLC1), transcript variant 2
ACCESSION   NM_006094
VERSION     NM_006094.3
SOURCE      synthetic construct 
FEATURES    Location/Qualifiers 
     misc_feature    201..924 
                     /label="CMV promoter"  
     misc_feature    839..856 
                     /label="Restriction Enzyme"  
     misc_feature    953..971 
                     /label="T7 promoter"  
     misc_feature    4311..4402 
                     /label="Myc-DDK"  
     misc_feature    4432..5017 
                     /label="PolyA signal"  
     misc_feature    4489..4508 
                     /label="Restriction Enzyme"  
     misc_feature    5293..5965 
                     /label="ColE1"  
     misc_feature    6395..7189 
                     /label="Kan/Neo"  
     misc_feature    7224..7526 
                     /label="SV40 ori"  
     misc_feature    7612..8089 
                     /label="f1 ori"  
     misc_feature    1020..1028 
                     /label="Restriction Enzyme"  
     misc_feature    4293..4299 
                     /label="Restriction Enzyme"  
     misc_feature    1020..4293 
                     /label="DLC1"  
ORIGIN                      
        1 AACAAAATAT TAACGCTTAC AATTTCCATT CGCCATTCAG GCTGCGCAAC TGTTGGGAAG 
       61 GGCGATCGGT GCGGGCCTCT TCGCTATTAC GCCAGCTGGC GAAAGGGGGA TGTGCTGCAA 
      121 GGCGATTAAG TTGGGTAACG CCAGGGTTTT CCCAGTCACG ACGTTGTAAA ACGACGGCCA 
      181 GTGCCAAGCT GATCTATACA TTGAATCAAT ATTGGCAATT AGCCATATTA GTCATTGGTT 
      241 ATATAGCATA AATCAATATT GGCTATTGGC CATTGCATAC GTTGTATCTA TATCATAATA 
      301 TGTACATTTA TATTGGCTCA TGTCCAATAT GACCGCCATG TTGACATTGA TTATTGACTA 
      361 GTTATTAATA GTAATCAATT ACGGGGTCAT TAGTTCATAG CCCATATATG GAGTTCCGCG 
      421 TTACATAACT TACGGTAAAT GGCCCGCCTG GCTGACCGCC CAACGACCCC CGCCCATTGA 
      481 CGTCAATAAT GACGTATGTT CCCATAGTAA CGCCAATAGG GACTTTCCAT TGACGTCAAT 
      541 GGGTGGAGTA TTTACGGTAA ACTGCCCACT TGGCAGTACA TCAAGTGTAT CATATGCCAA 
      601 GTCCGCCCCC TATTGACGTC AATGACGGTA AATGGCCCGC CTGGCATTAT GCCCAGTACA 
      661 TGACCTTACG GGACTTTCCT ACTTGGCAGT ACATCTACGT ATTAGTCATC GCTATTACCA 
      721 TGGTGATGCG GTTTTGGCAG TACACCAATG GGCGTGGATA GCGGTTTGAC TCACGGGGAT 
      781 TTCCAAGTCT CCACCCCATT GACGTCAATG GGAGTTTGTT TTGGCACCAA AATCAACGGG 
      841 ACTTTCCAAA ATGTCGTAAT AACCCCGCCC CGTTGACGCA AATGGGCGGT AGGCGTGTAC 
      901 GGTGGGAGGT CTATATAAGC AGAGCTCGTT TAGTGAACCG TCAGAATTTT GTAATACGAC 
      961 TCACTATAGG GCGGCCGGGA ATTCGTCGAC TGGATCCGGT ACCGAGGAGA TCTGCCGCCG 
     1021 CGATCGCCAT GTGCAGAAAG AAGCCGGACA CCATGATCCT AACACAAATT GAAGCCAAGG 
     1081 AAGCTTGTGA TTGGCTACGG GCAACTGGTT TCCCCCAGTA TGCACAGCTT TATGAAGATT 
     1141 TCCTGTTCCC CATCGATATT TCCTTGGTCA AGAGAGAGCA TGATTTTTTG GACAGAGATG 
     1201 CCATTGAGGC TCTATGCAGG CGTCTAAATA CTTTAAACAA ATGTGCGGTG ATGAAGCTAG 
     1261 AAATTAGTCC TCATCGGAAA CGAAGTGACG ATTCAGACGA GGATGAGCCT TGTGCCATCA 
     1321 GTGGCAAATG GACTTTCCAA AGGGACAGCA AGAGGTGGTC CCGGCTTGAA GAGTTTGATG 
     1381 TCTTTTCTCC AAAACAAGAC CTGGTCCCTG GGTCCCCAGA CGACTCCCAC CCGAAGGACG 
     1441 GCCCCAGCCC CGGAGGCACG CTGATGGACC TCAGCGAGCG CCAGGAGGTG TCTTCCGTCC 
     1501 GCAGCCTCAG CAGCACTGGC AGCCTCCCCA GCCACGCGCC CCCCAGCGAG GATGCTGCCA 
     1561 CCCCCCGGAC TAACTCCGTC ATCAGCGTTT GCTCCTCCAG CAACTTGGCA GGCAATGACG 
     1621 ACTCTTTCGG CAGCCTGCCC TCTCCCAAGG AACTGTCCAG CTTCAGCTTC AGCATGAAAG 
     1681 GCCACGAAAA AACTGCCAAG TCCAAGACGC GCAGTCTGCT GAAACGGATG GAGAGCCTGA 
     1741 AGCTCAAGAG CTCCCATCAC AGCAAGCACA AAGCGCCCTC AAAGCTGGGG TTGATCATCA 
     1801 GCGGGCCCAT CTTGCAAGAG GGGATGGATG AGGAGAAGCT GAAGCAGCTC AACTGCGTGG 
     1861 AGATCTCCGC CCTCAATGGC AACCGCATCA ACGTCCCCAT GGTACGAAAG AGGAGCGTTT 
     1921 CCAACTCCAC GCAGACCAGC AGCAGCAGCA GCCAGTCGGA GACCAGCAGC GCGGTCAGCA 
     1981 CGCCCAGCCC TGTTACGAGG ACCCGGAGCC TCAGTGCGTG CAACAAGCGG GTGGGCATGT 
     2041 ACTTAGAGGG CTTCGATCCT TTCAATCAGT CAACATTTAA CAACGTGATG GAGCAGAACT 
     2101 TTAAGAACCG CGAGAGCTAC CCAGAGGACA CGGTGTTCTA CATCCCTGAA GATCACAAGC 
     2161 CTGGCACTTT CCCCAAAGCT CTCACCAATG GCAGTTTCTC CCCCTCGGGG AATAACGGCT 
     2221 CTGTGAACTG GAGGACGGGA AGCTTCCACG GCCCTGGCCA CATCAGCCTC AGGAGGGAAA 
     2281 ACAGTAGCGA CAGCCCCAAG GAACTGAAGA GACGCAATTC TTCCAGCTCC ATGAGCAGCC 
     2341 GCCTGAGCAT CTACGACAAC GTGCCGGGCT CCATCCTCTA CTCCAGTTCA GGGGACCTGG 
     2401 CGGATCTGGA GAACGAGGAC ATCTTCCCCG AGCTGGACGA CATCCTCTAC CACGTGAAGG 
     2461 GGATGCAGCG GATAGTCAAT CAGTGGTCGG AGAAGTTTTC TGATGAGGGA GATTCGGACT 
     2521 CAGCCCTGGA CTCGGTCTCT CCCTGCCCGT CCTCTCCAAA ACAGATACAC CTGGATGTGG 
     2581 ACAACGACCG AACCACACCC AGCGACCTGG ACAGCACAGG CAACTCCCTG AATGAACCGG 
     2641 AAGAGCCCTC CGAGATCCCG GAAAGAAGGG ATTCTGGGGT TGGGGCTTCC CTAACCAGGT 
     2701 CCAACAGGCA CCGACTGAGA TGGCACAGTT TCCAGAGCTC ACATCGGCCA AGCCTCAACT 
     2761 CTGTATCACT ACAGATTAAC TGCCAGTCTG TGGCCCAGAT GAACCTGCTG CAGAAATACT 
     2821 CACTCCTAAA GCTAACGGCC CTGCTGGAGA AATACACACC TTCTAACAAG CATGGTTTTA 
     2881 GCTGGGCCGT GCCCAAGTTC ATGAAGAGGA TCAAGGTTCC AGACTACAAG GACCGGAGTG 
     2941 TGTTTGGGGT CCCACTGACG GTCAACGTGC AGCGCACAGG ACAACCGTTG CCTCAGAGCA 
     3001 TCCAGCAGGC CATGCGATAC CTCCGGAACC ATTGTTTGGA TCAGGTTGGG CTCTTCAGAA 
     3061 AATCGGGGGT CAAGTCCCGG ATTCAGGCTC TGCGCCAGAT GAATGAAGGT GCCATAGACT 
     3121 GTGTCAACTA CGAAGGACAG TCTGCTTATG ACGTGGCAGA CATGCTGAAG CAGTATTTTC 
     3181 GAGATCTTCC TGAGCCACTA ATGACGAACA AACTCTCGGA AACCTTTCTA CAGATCTACC 
     3241 AATATGTGCC CAAGGACCAG CGCCTGCAGG CCATCAAGGC TGCCATCATG CTGCTGCCTG 
     3301 ACGAGAACCG GGAGGTTCTG CAGACCCTGC TTTATTTCCT GAGCGATGTC ACAGCAGCCG 
     3361 TAAAAGAAAA CCAGATGACC CCAACCAACC TGGCCGTGTG CTTAGCGCCT TCCCTCTTCC 
     3421 ATCTCAACAC CCTGAAGAGA GAGAATTCCT CTCCCAGGGT AATGCAAAGA AAACAAAGTT 
     3481 TGGGCAAACC AGATCAGAAA GATTTGAATG AAAACCTAGC TGCCACTCAA GGGCTGGCCC 
     3541 ATATGATCGC CGAGTGCAAG AAGCTTTTCC AGGTTCCCGA GGAAATGAGC CGATGTCGTA 
     3601 ATTCCTATAC CGAACAAGAG CTGAAGCCCC TCACTCTGGA AGCACTCGGG CACCTGGGTA 
     3661 ATGATGACTC AGCTGACTAC CAACACTTCC TCCAGGACTG TGTGGATGGC CTGTTTAAAG 
     3721 AAGTCAAAGA GAAGTTTAAA GGCTGGGTCA GCTACTCCAC TTCGGAGCAG GCTGAGCTGT 
     3781 CCTATAAGAA GGTGAGCGAA GGACCCCCTC TGAGGCTTTG GAGGTCAGTC ATTGAAGTCC 
     3841 CTGCTGTGCC AGAGGAAATC TTAAAGCGCC TACTTAAAGA ACAGCACCTC TGGGATGTAG 
     3901 ACCTGTTGGA TTCAAAAGTG ATCGAAATTC TGGACAGCCA AACTGAAATT TACCAGTATG 
     3961 TCCAAAACAG TATGGCACCT CATCCTGCTC GAGACTACGT TGTTTTAAGA ACCTGGAGGA 
     4021 CTAATTTACC CAAAGGAGCC TGTGCCCTTT TACTAACCTC TGTGGATCAC GATCGCGCAC 
     4081 CTGTGGTGGG TGTGAGGGTT AATGTGCTCT TGTCCAGGTA TTTGATTGAA CCCTGTGGGC 
     4141 CAGGAAAATC CAAACTCACC TACATGTGCA GAGTTGACTT AAGGGGCCAC ATGCCAGAAT 
     4201 GGTACACAAA ATCTTTTGGA CATTTGTGTG CAGCTGAAGT TGTAAAGATC CGGGATTCCT 
     4261 TCAGTAACCA GAACACTGAA ACCAAAGACA CCAAATCTAG GACGCGTACG CGGCCGCTCG 
     4321 AGCAGAAACT CATCTCAGAA GAGGATCTGG CAGCAAATGA TATCCTGGAT TACAAGGATG 
     4381 ACGACGATAA GGTTTAAACG GCCGGCCGCG GTCATAGCTG TTTCCTGAAC AGATCCCGGG 
     4441 TGGCATCCCT GTGACCCCTC CCCAGTGCCT CTCCTGGCCC TGGAAGTTGC CACTCCAGTG 
     4501 CCCACCAGCC TTGTCCTAAT AAAATTAAGT TGCATCATTT TGTCTGACTA GGTGTCCTTC 
     4561 TATAATATTA TGGGGTGGAG GGGGGTGGTA TGGAGCAAGG GGCAAGTTGG GAAGACAACC 
     4621 TGTAGGGCCT GCGGGGTCTA TTGGGAACCA AGCTGGAGTG CAGTGGCACA ATCTTGGCTC 
     4681 ACTGCAATCT CCGCCTCCTG GGTTCAAGCG ATTCTCCTGC CTCAGCCTCC CGAGTTGTTG 
     4741 GGATTCCAGG CATGCATGAC CAGGCTCAGC TAATTTTTGT TTTTTTGGTA GAGACGGGGT 
     4801 TTCACCATAT TGGCCAGGCT GGTCTCCAAC TCCTAATCTC AGGTGATCTA CCCACCTTGG 
     4861 CCTCCCAAAT TGCTGGGATT ACAGGCGTGA ACCACTGCTC CCTTCCCTGT CCTTCTGATT 
     4921 TTAAAATAAC TATACCAGCA GGAGGACGTC CAGACACAGC ATAGGCTACC TGGCCATGCC 
     4981 CAACCGGTGG GACATTTGAG TTGCTTGCTT GGCACTGTCC TCTCATGCGT TGGGTCCACT 
     5041 CAGTAGATGC CTGTTGAATT GGGTACGCGG CCAGCGGCGA GCGGTATCAG CTCACTCAAA 
     5101 GGCGGTAATA CGGTTATCCA CAGAATCAGG GGATAACGCA GGAAAGAACA TGTGAGCAAA 
     5161 AGGCCAGCAA AAGGCCAGGA ACCGTAAAAA GGCCGCGTTG CTGGCGTTTT TCCATAGGCT 
     5221 CCGCCCCCCT GACGAGCATC ACAAAAATCG ACGCTCAAGT CAGAGGTGGC GAAACCCGAC 
     5281 AGGACTATAA AGATACCAGG CGTTTCCCCC TGGAAGCTCC CTCGTGCGCT CTCCTGTTCC 
     5341 GACCCTGCCG CTTACCGGAT ACCTGTCCGC CTTTCTCCCT TCGGGAAGCG TGGCGCTTTC 
     5401 TCATAGCTCA CGCTGTAGGT ATCTCAGTTC GGTGTAGGTC GTTCGCTCCA AGCTGGGCTG 
     5461 TGTGCACGAA CCCCCCGTTC AGCCCGACCG CTGCGCCTTA TCCGGTAACT ATCGTCTTGA 
     5521 GTCCAACCCG GTAAGACACG ACTTATCGCC ACTGGCAGCA GCCACTGGTA ACAGGATTAG 
     5581 CAGAGCGAGG TATGTAGGCG GTGCTACAGA GTTCTTGAAG TGGTGGCCTA ACTACGGCTA 
     5641 CACTAGAAGA ACAGTATTTG GTATCTGCGC TCTGCTGAAG CCAGTTACCT TCGGAAAAAG 
     5701 AGTTGGTAGC TCTTGATCCG GCAAACAAAC CACCGCTGGT AGCGGTGGTT TTTTTGTTTG 
     5761 CAAGCAGCAG ATTACGCGCA GAAAAAAAGG ATCTCAAGAA GATCCTTTGA TCTTTTCTAC 
     5821 GGGGTCTGAC GCTCAGTGGA ACGAAAACTC ACGTTAAGGG ATTTTGGTCA TGAGATTATC 
     5881 AAAAAGGATC TTCACCTAGA TCCTTTTAAA TTAAAAATGA AGTTTTAAAT CAATCTAAAG 
     5941 TATATATGAG TAACCTGAGG CTATGGCAGG GCCTGCCGCC CCGACGTTGG CTGCGAGCCC 
     6001 TGGGCCTTCA CCCGAACTTG GGGGGTGGGG TGGGGAAAAG GAAGAAACGC GGGCGTATTG 
     6061 GCCCCAATGG GGTCTCGGTG GGGTATCGAC AGAGTGCCAG CCCTGGGACC GAACCCCGCG 
     6121 TTTATGAACA AACGACCCAA CACCGTGCGT TTTATTCTGT CTTTTTATTG CCGTCATAGC 
     6181 GCGGGTTCCT TCCGGTATTG TCTCCTTCCG TGTTTCAGTT AGCCTCCCCC TAGGGTGGGC 
     6241 GAAGAACTCC AGCATGAGAT CCCCGCGCTG GAGGATCATC CAGCCGGCGT CCCGGAAAAC 
     6301 GATTCCGAAG CCCAACCTTT CATAGAAGGC GGCGGTGGAA TCGAAATCTC GTGATGGCAG 
     6361 GTTGGGCGTC GCTTGGTCGG TCATTTCGAA CCCCAGAGTC CCGCTCAGAA GAACTCGTCA 
     6421 AGAAGGCGAT AGAAGGCGAT GCGCTGCGAA TCGGGAGCGG CGATACCGTA AAGCACGAGG 
     6481 AAGCGGTCAG CCCATTCGCC GCCAAGCTCT TCAGCAATAT CACGGGTAGC CAACGCTATG 
     6541 TCCTGATAGC GATCCGCCAC ACCCAGCCGG CCACAGTCGA TGAATCCAGA AAAGCGGCCA 
     6601 TTTTCCACCA TGATATTCGG CAAGCAGGCA TCGCCATGGG TCACGACGAG ATCCTCGCCG 
     6661 TCGGGCATGC TCGCCTTGAG CCTGGCGAAC AGTTCGGCTG GCGCGAGCCC CTGATGCTCT 
     6721 TCGTCCAGAT CATCCTGATC GACAAGACCG GCTTCCATCC GAGTACGTGC TCGCTCGATG 
     6781 CGATGTTTCG CTTGGTGGTC GAATGGGCAG GTAGCCGGAT CAAGCGTATG CAGCCGCCGC 
     6841 ATTGCATCAG CCATGATGGA TACTTTCTCG GCAGGAGCAA GGTGAGATGA CAGGAGATCC 
     6901 TGCCCCGGCA CTTCGCCCAA TAGCAGCCAG TCCCTTCCCG CTTCAGTGAC AACGTCGAGC 
     6961 ACAGCTGCGC AAGGAACGCC CGTCGTGGCC AGCCACGATA GCCGCGCTGC CTCGTCTTGC 
     7021 AGTTCATTCA GGGCACCGGA CAGGTCGGTC TTGACAAAAA GAACCGGGCG CCCCTGCGCT 
     7081 GACAGCCGGA ACACGGCGGC ATCAGAGCAG CCGATTGTCT GTTGTGCCCA GTCATAGCCG 
     7141 AATAGCCTCT CCACCCAAGC GGCCGGAGAA CCTGCGTGCA ATCCATCTTG TTCAATCATG 
     7201 CGAAACGATC CTCATCCTGT CTCTTGATCG ATCTTTGCAA AAGCCTAGGC CTCCAAAAAA 
     7261 GCCTCCTCAC TACTTCTGGA ATAGCTCAGA GGCCGAGGCG GCCTCGGCCT CTGCATAAAT 
     7321 AAAAAAAATT AGTCAGCCAT GGGGCGGAGA ATGGGCGGAA CTGGGCGGAG TTAGGGGCGG 
     7381 GATGGGCGGA GTTAGGGGCG GGACTATGGT TGCTGACTAA TTGAGATGCA TGCTTTGCAT 
     7441 ACTTCTGCCT GCTGGGGAGC CTGGGGACTT TCCACACCTG GTTGCTGACT AATTGAGATG 
     7501 CATGCTTTGC ATACTTCTGC CTGCTGGGGA GCCTGGGGAC TTTCCACACC CTAACTGACA 
     7561 CACATTCCAC AGCTGGTTCT TTCCGCCTCA GGACTCTTCC TTTTTCAATA TTATTGAAGC 
     7621 ATTTATCAGG GTTATTGTCT CATGAGCGGA TACATATTTG AATGTATTTA GAAAAATAAA 
     7681 CAAATAGGGG TTCCGCGCAC ATTTCCCCGA AAAGTGCCAC CTGACGCGCC CTGTAGCGGC 
     7741 GCATTAAGCG CGGCGGGTGT GGTGGTTACG CGCAGCGTGA CCGCTACACT TGCCAGCGCC 
     7801 CTAGCGCCCG CTCCTTTCGC TTTCTTCCCT TCCTTTCTCG CCACGTTCGC CGGCTTTCCC 
     7861 CGTCAAGCTC TAAATCGGGG GCTCCCTTTA GGGTTCCGAT TTAGTGCTTT ACGGCACCTC 
     7921 GACCCCAAAA AACTTGATTA GGGTGATGGT TCACGTAGTG GGCCATCGCC CTGATAGACG 
     7981 GTTTTTCGCC CTTTGACGTT GGAGTCCACG TTCTTTAATA GTGGACTCTT GTTCCAAACT 
     8041 GGAACAACAC TCAACCCTAT CTCGGTCTAT TCTTTTGATT TATAAGGGAT TTTGCCGATT 
     8101 TCGGCCTATT GGTTAAAAAA TGAGCTGATT TAACAAAAAT TTAACGCGAA TTTT
//