LOCUS       RC213278                8448 bp    DNA     circular  SYN 
DEFINITION  DIDO1 (Myc-DDK-tagged)-Human death inducer-obliterator 1 (DIDO1), transcript variant 3
ACCESSION   NM_080797
VERSION     NM_080797.3
SOURCE      synthetic construct 
FEATURES    Location/Qualifiers 
     misc_feature    201..924 
                     /label="CMV promoter"  
     misc_feature    839..856 
                     /label="Restriction Enzyme"  
     misc_feature    953..971 
                     /label="T7 promoter"  
     misc_feature    4605..4696 
                     /label="Myc-DDK"  
     misc_feature    4726..5311 
                     /label="PolyA signal"  
     misc_feature    4783..4802 
                     /label="Restriction Enzyme"  
     misc_feature    5587..6259 
                     /label="ColE1"  
     misc_feature    6689..7483 
                     /label="Kan/Neo"  
     misc_feature    7518..7820 
                     /label="SV40 ori"  
     misc_feature    7906..8383 
                     /label="f1 ori"  
     misc_feature    1020..1028 
                     /label="Restriction Enzyme"  
     misc_feature    4587..4593 
                     /label="Restriction Enzyme"  
     misc_feature    1020..4587 
                     /label="DIDO1"  
ORIGIN                      
        1 AACAAAATAT TAACGCTTAC AATTTCCATT CGCCATTCAG GCTGCGCAAC TGTTGGGAAG 
       61 GGCGATCGGT GCGGGCCTCT TCGCTATTAC GCCAGCTGGC GAAAGGGGGA TGTGCTGCAA 
      121 GGCGATTAAG TTGGGTAACG CCAGGGTTTT CCCAGTCACG ACGTTGTAAA ACGACGGCCA 
      181 GTGCCAAGCT GATCTATACA TTGAATCAAT ATTGGCAATT AGCCATATTA GTCATTGGTT 
      241 ATATAGCATA AATCAATATT GGCTATTGGC CATTGCATAC GTTGTATCTA TATCATAATA 
      301 TGTACATTTA TATTGGCTCA TGTCCAATAT GACCGCCATG TTGACATTGA TTATTGACTA 
      361 GTTATTAATA GTAATCAATT ACGGGGTCAT TAGTTCATAG CCCATATATG GAGTTCCGCG 
      421 TTACATAACT TACGGTAAAT GGCCCGCCTG GCTGACCGCC CAACGACCCC CGCCCATTGA 
      481 CGTCAATAAT GACGTATGTT CCCATAGTAA CGCCAATAGG GACTTTCCAT TGACGTCAAT 
      541 GGGTGGAGTA TTTACGGTAA ACTGCCCACT TGGCAGTACA TCAAGTGTAT CATATGCCAA 
      601 GTCCGCCCCC TATTGACGTC AATGACGGTA AATGGCCCGC CTGGCATTAT GCCCAGTACA 
      661 TGACCTTACG GGACTTTCCT ACTTGGCAGT ACATCTACGT ATTAGTCATC GCTATTACCA 
      721 TGGTGATGCG GTTTTGGCAG TACACCAATG GGCGTGGATA GCGGTTTGAC TCACGGGGAT 
      781 TTCCAAGTCT CCACCCCATT GACGTCAATG GGAGTTTGTT TTGGCACCAA AATCAACGGG 
      841 ACTTTCCAAA ATGTCGTAAT AACCCCGCCC CGTTGACGCA AATGGGCGGT AGGCGTGTAC 
      901 GGTGGGAGGT CTATATAAGC AGAGCTCGTT TAGTGAACCG TCAGAATTTT GTAATACGAC 
      961 TCACTATAGG GCGGCCGGGA ATTCGTCGAC TGGATCCGGT ACCGAGGAGA TCTGCCGCCG 
     1021 CGATCGCCAT GGACGACAAA GGCGACCCGA GCAATGAGGA GGCACCTAAG GCCATCAAAC 
     1081 CCACCAGCAA AGAGTTCAGG AAAACATGGG GTTTTCGAAG GACCACTATC GCCAAGCGAG 
     1141 AGGGCGCAGG GGACGCGGAG GCTGACCCAC TGGAGCCGCC ACCCCCACAG CAGCAGCTGG 
     1201 GCCTGTCCCT GCGGCGCAGT GGGAGGCAGC CCAAGCGCAC TGAGCGCGTG GAGCAGTTCC 
     1261 TGACCATTGC GCGGCGCCGC GGCAGGAGGA GCATGCCTGT CTCCCTGGAG GATTCTGGTG 
     1321 AGCCCACGTC CTGCCCCGCC ACAGACGCCG AGACAGCCTC CGAGGGCAGC GTGGAAAGCG 
     1381 CTTCTGAGAC CAGAAGCGGC CCCCAGTCTG CTTCCACAGC TGTGAAGGAA CGACCAGCCT 
     1441 CTTCTGAAAA GGTGAAAGGA GGGGATGACC ACGATGACAC CTCCGATAGT GACAGCGATG 
     1501 GCCTGACCTT GAAAGAGCTT CAGAATCGCC TTCGCAGGAA GCGGGAACAG GAGCCCACTG 
     1561 AGAGGCCCCT GAAAGGGATC CAGAGTCGCC TGCGGAAGAA GCGCCGGGAG GAGGGTCCCG 
     1621 CCGAGACTGT GGGCTCCGAG GCCAGTGACA CTGTGGAGGG CGTCCTGCCC AGTAAGCAGG 
     1681 AGCCCGAGAA CGATCAGGGG GTTGTGTCCC AGGCTGGGAA AGATGACAGA GAGAGTAAGT 
     1741 TGGAGGGAAA GGCGGCTCAG GACATCAAAG ATGAGGAGCC TGGAGACTTG GGCCGACCGA 
     1801 AGCCTGAATG TGAGGGTTAC GACCCCAACG CCCTGTATTG CATTTGCCGC CAGCCTCACA 
     1861 ACAACAGGTT TATGATTTGC TGTGACCGCT GTGAAGAATG GTTTCATGGC GATTGTGTGG 
     1921 GCATTTCTGA GGCTCGAGGG AGGCTTTTGG AAAGGAATGG GGAAGACTAT ATCTGCCCAA 
     1981 ACTGCACCAT TCTGCAAGTG CAGGATGAGA CTCATTCAGA AACGGCAGAT CAGCAGGAAG 
     2041 CTAAATGGAG ACCTGGAGAT GCTGATGGCA CCGATTGTAC AAGTATAGGA ACAATAGAGC 
     2101 AGAAGTCTAG CGAAGACCAA GGGATAAAGG GTAGAATTGA GAAAGCTGCA AATCCAAGTG 
     2161 GCAAGAAGAA ACTCAAGATC TTCCAGCCTG TGATAGAGGC GCCTGGTGCC TCAAAATGTA 
     2221 TTGGCCCCGG GTGCTGTCAC GTGGCGCAGC CCGACTCGGT GTACTGCAGT AATGACTGTA 
     2281 TCCTCAAACA CGCCGCAGCG ACAATGAAGT TTCTAAGCTC AGGTAAAGAA CAGAAGCCAA 
     2341 AGCCTAAAGA AAAGATGAAG ATGAAGCCAG AGAAGCCCAG TCTTCCGAAA TGCGGTGCTC 
     2401 AGGCAGGTAT TAAAATCTCT TCTGTGCACA AGAGACCAGC TCCAGAAAAA AAAGAGACCA 
     2461 CAGTGAAGAA GGCAGTGGTG GTCCCTGCGC GGAGTGAAGC ACTCGGGAAG GAAGCAGCTT 
     2521 GTGAGAGCAG CACGCCGTCG TGGGCGAGCG ATCACAATTA CAATGCAGTA AAGCCAGAAA 
     2581 AGACTGCTGC TCCCTCGCCG TCACTGTTGT ATAAATCCAC GAAGGAAGAC AGGAGGTCCG 
     2641 AGGAGAAAGC GGCAGCCATG GCAGCCTCAA AGAAAACAGC CCCTCCAGGC TCCGCGGTGG 
     2701 GCAAGCAGCC TGCACCTAGA AACCTCGTGC CAAAGAAGTC TTCTTTTGCT AATGTGGCAG 
     2761 CAGCCACACC AGCCATTAAA AAGCCACCCT CAGGTTTCAA GGGCACCATC CCCAAGAGGC 
     2821 CATGGCTCTC CGCTACCCCA TCGAGTGGTG CTTCAGCTGC CAGGCAGGCC GGACCGGCAC 
     2881 CTGCAGCGGC AACGGCTGCC TCCAAGAAGT TCCCTGGCTC CGCTGCTTTG GTGGGAGCCG 
     2941 TAAGGAAGCC AGTGGTACCT TCTGTTCCAA TGGCCTCGCC AGCCCCAGGA CGCCTTGGGG 
     3001 CTATGAGTGC TGCACCATCG CAGCCAAATT CACAAATTCG GCAAAATATC AGACGCTCCT 
     3061 TAAAAGAGAT TTTGTGGAAA AGAGTCAATG ACAGCGATGA CTTAATCATG ACAGAAAACG 
     3121 AAGTAGGAAA AATTGCCCTC CATATTGAGA AGGAGATGTT TAACTTGTTT CAAGTTACAG 
     3181 ATAATCGCTA CAAGAGTAAA TATCGCAGCA TCATGTTCAA CCTGAAGGAC CCTAAAAATC 
     3241 AGGGACTCTT CCATCGTGTT CTGCGTGAGG AAATCTCTTT GGCGAAACTT GTGAGACTGA 
     3301 AGCCAGAAGA ACTTGTATCT AAAGAGCTTT CCACGTGGAA AGAGAGGCCA GCGAGATCTG 
     3361 TGATGGAGTC CAGAACTAAA CTGCACAATG AAAGCAAGAA GACGGCCCCC AGGCAGGAGG 
     3421 CCATCCCCGA TCTGGAGGAC TCTCCGCCAG TGTCGGATTC AGAGGAACAG CAAGAGTCAG 
     3481 CACGTGCTGT CCCTGAGAAG AGCACAGCGC CGCTTCTCGA CGTCTTCAGC AGCATGTTGA 
     3541 AAGACACCAC CAGTCAGCAC CGCGCACACC TCTTCGATCT CAACTGTAAA ATTTGCACAG 
     3601 GCCAGGTTCC CTCCGCAGAA GATGAGCCAG CTCCGAAAAA ACAAAAATTG TCAGCTTCTG 
     3661 TTAAGAAAGA AGACTTAAAA TCAAAGCATG ACAGCTCTGC ACCTGACCCA GCTCCGGATT 
     3721 CAGCTGATGA GGTGATGCCG GAGGCTGTGC CTGAAGTTGC CTCTGAGCCA GGCCTAGAAA 
     3781 GTGCTTCTCA TCCAAATGTG GACAGAACGT ATTTCCCTGG GCCTCCAGGA GATGGCCATC 
     3841 CCGAGCCCTC CCCGCTGGAA GACCTGTCCC CCTGCCCAGC CTCCTGTGGG AGCGGGGTGG 
     3901 TCACCACCGT CACAGTGTCC GGCCGGGACC CCAGGACCGC TCCAAGCAGT TCATGCACAG 
     3961 CCGTGGCCTC CGCAGCATCC CGCCCAGACA GCACCCACAT GGTGGAAGCC AGACAGGATG 
     4021 TGCCGAAGCC TGTCTTGACT TCTGTGATGG TGCCCAAGTC CATACTAGCT AAGCCATCCT 
     4081 CATCTCCTGA CCCAAGATAC CTGTCAGTTC CTCCGTCACC AAATATCAGC ACTTCAGAAT 
     4141 CACGTTCCCC TCCAGAGGGA GACACGACCC TCTTTTTGTC TCGACTCAGC ACCATTTGGA 
     4201 AAGGATTTAT TAACATGCAG AGTGTGGCAA AATTTGTCAC TAAGGCGTAT CCTGTCTCTG 
     4261 GGTGTTTTGA TTACCTCAGT GAGGATTTGC CTGACACAAT TCACATTGGT GGGAGGATCG 
     4321 CACCGAAGAC AGTTTGGGAT TATGTTGGCA AACTCAAGTC TTCTGTGTCT AAGGAGCTCT 
     4381 GTCTGATCCG CTTCCACCCC GCCACAGAGG AAGAGGAGGT CGCCTATATC TCTCTCTACT 
     4441 CCTATTTCAG CAGCCGTGGC CGCTTTGGTG TTGTAGCTAA TAACAACAGG CACGTCAAGG 
     4501 ACCTCTACCT GATCCCGCTG AGCGCCCAGG ACCCTGTTCC ATCCAAACTC TTGCCCTTTG 
     4561 AGGGACCAGG TAAGCGCCGG CTTTCTGGGT GGAGGACGCG TACGCGGCCG CTCGAGCAGA 
     4621 AACTCATCTC AGAAGAGGAT CTGGCAGCAA ATGATATCCT GGATTACAAG GATGACGACG 
     4681 ATAAGGTTTA AACGGCCGGC CGCGGTCATA GCTGTTTCCT GAACAGATCC CGGGTGGCAT 
     4741 CCCTGTGACC CCTCCCCAGT GCCTCTCCTG GCCCTGGAAG TTGCCACTCC AGTGCCCACC 
     4801 AGCCTTGTCC TAATAAAATT AAGTTGCATC ATTTTGTCTG ACTAGGTGTC CTTCTATAAT 
     4861 ATTATGGGGT GGAGGGGGGT GGTATGGAGC AAGGGGCAAG TTGGGAAGAC AACCTGTAGG 
     4921 GCCTGCGGGG TCTATTGGGA ACCAAGCTGG AGTGCAGTGG CACAATCTTG GCTCACTGCA 
     4981 ATCTCCGCCT CCTGGGTTCA AGCGATTCTC CTGCCTCAGC CTCCCGAGTT GTTGGGATTC 
     5041 CAGGCATGCA TGACCAGGCT CAGCTAATTT TTGTTTTTTT GGTAGAGACG GGGTTTCACC 
     5101 ATATTGGCCA GGCTGGTCTC CAACTCCTAA TCTCAGGTGA TCTACCCACC TTGGCCTCCC 
     5161 AAATTGCTGG GATTACAGGC GTGAACCACT GCTCCCTTCC CTGTCCTTCT GATTTTAAAA 
     5221 TAACTATACC AGCAGGAGGA CGTCCAGACA CAGCATAGGC TACCTGGCCA TGCCCAACCG 
     5281 GTGGGACATT TGAGTTGCTT GCTTGGCACT GTCCTCTCAT GCGTTGGGTC CACTCAGTAG 
     5341 ATGCCTGTTG AATTGGGTAC GCGGCCAGCG GCGAGCGGTA TCAGCTCACT CAAAGGCGGT 
     5401 AATACGGTTA TCCACAGAAT CAGGGGATAA CGCAGGAAAG AACATGTGAG CAAAAGGCCA 
     5461 GCAAAAGGCC AGGAACCGTA AAAAGGCCGC GTTGCTGGCG TTTTTCCATA GGCTCCGCCC 
     5521 CCCTGACGAG CATCACAAAA ATCGACGCTC AAGTCAGAGG TGGCGAAACC CGACAGGACT 
     5581 ATAAAGATAC CAGGCGTTTC CCCCTGGAAG CTCCCTCGTG CGCTCTCCTG TTCCGACCCT 
     5641 GCCGCTTACC GGATACCTGT CCGCCTTTCT CCCTTCGGGA AGCGTGGCGC TTTCTCATAG 
     5701 CTCACGCTGT AGGTATCTCA GTTCGGTGTA GGTCGTTCGC TCCAAGCTGG GCTGTGTGCA 
     5761 CGAACCCCCC GTTCAGCCCG ACCGCTGCGC CTTATCCGGT AACTATCGTC TTGAGTCCAA 
     5821 CCCGGTAAGA CACGACTTAT CGCCACTGGC AGCAGCCACT GGTAACAGGA TTAGCAGAGC 
     5881 GAGGTATGTA GGCGGTGCTA CAGAGTTCTT GAAGTGGTGG CCTAACTACG GCTACACTAG 
     5941 AAGAACAGTA TTTGGTATCT GCGCTCTGCT GAAGCCAGTT ACCTTCGGAA AAAGAGTTGG 
     6001 TAGCTCTTGA TCCGGCAAAC AAACCACCGC TGGTAGCGGT GGTTTTTTTG TTTGCAAGCA 
     6061 GCAGATTACG CGCAGAAAAA AAGGATCTCA AGAAGATCCT TTGATCTTTT CTACGGGGTC 
     6121 TGACGCTCAG TGGAACGAAA ACTCACGTTA AGGGATTTTG GTCATGAGAT TATCAAAAAG 
     6181 GATCTTCACC TAGATCCTTT TAAATTAAAA ATGAAGTTTT AAATCAATCT AAAGTATATA 
     6241 TGAGTAACCT GAGGCTATGG CAGGGCCTGC CGCCCCGACG TTGGCTGCGA GCCCTGGGCC 
     6301 TTCACCCGAA CTTGGGGGGT GGGGTGGGGA AAAGGAAGAA ACGCGGGCGT ATTGGCCCCA 
     6361 ATGGGGTCTC GGTGGGGTAT CGACAGAGTG CCAGCCCTGG GACCGAACCC CGCGTTTATG 
     6421 AACAAACGAC CCAACACCGT GCGTTTTATT CTGTCTTTTT ATTGCCGTCA TAGCGCGGGT 
     6481 TCCTTCCGGT ATTGTCTCCT TCCGTGTTTC AGTTAGCCTC CCCCTAGGGT GGGCGAAGAA 
     6541 CTCCAGCATG AGATCCCCGC GCTGGAGGAT CATCCAGCCG GCGTCCCGGA AAACGATTCC 
     6601 GAAGCCCAAC CTTTCATAGA AGGCGGCGGT GGAATCGAAA TCTCGTGATG GCAGGTTGGG 
     6661 CGTCGCTTGG TCGGTCATTT CGAACCCCAG AGTCCCGCTC AGAAGAACTC GTCAAGAAGG 
     6721 CGATAGAAGG CGATGCGCTG CGAATCGGGA GCGGCGATAC CGTAAAGCAC GAGGAAGCGG 
     6781 TCAGCCCATT CGCCGCCAAG CTCTTCAGCA ATATCACGGG TAGCCAACGC TATGTCCTGA 
     6841 TAGCGATCCG CCACACCCAG CCGGCCACAG TCGATGAATC CAGAAAAGCG GCCATTTTCC 
     6901 ACCATGATAT TCGGCAAGCA GGCATCGCCA TGGGTCACGA CGAGATCCTC GCCGTCGGGC 
     6961 ATGCTCGCCT TGAGCCTGGC GAACAGTTCG GCTGGCGCGA GCCCCTGATG CTCTTCGTCC 
     7021 AGATCATCCT GATCGACAAG ACCGGCTTCC ATCCGAGTAC GTGCTCGCTC GATGCGATGT 
     7081 TTCGCTTGGT GGTCGAATGG GCAGGTAGCC GGATCAAGCG TATGCAGCCG CCGCATTGCA 
     7141 TCAGCCATGA TGGATACTTT CTCGGCAGGA GCAAGGTGAG ATGACAGGAG ATCCTGCCCC 
     7201 GGCACTTCGC CCAATAGCAG CCAGTCCCTT CCCGCTTCAG TGACAACGTC GAGCACAGCT 
     7261 GCGCAAGGAA CGCCCGTCGT GGCCAGCCAC GATAGCCGCG CTGCCTCGTC TTGCAGTTCA 
     7321 TTCAGGGCAC CGGACAGGTC GGTCTTGACA AAAAGAACCG GGCGCCCCTG CGCTGACAGC 
     7381 CGGAACACGG CGGCATCAGA GCAGCCGATT GTCTGTTGTG CCCAGTCATA GCCGAATAGC 
     7441 CTCTCCACCC AAGCGGCCGG AGAACCTGCG TGCAATCCAT CTTGTTCAAT CATGCGAAAC 
     7501 GATCCTCATC CTGTCTCTTG ATCGATCTTT GCAAAAGCCT AGGCCTCCAA AAAAGCCTCC 
     7561 TCACTACTTC TGGAATAGCT CAGAGGCCGA GGCGGCCTCG GCCTCTGCAT AAATAAAAAA 
     7621 AATTAGTCAG CCATGGGGCG GAGAATGGGC GGAACTGGGC GGAGTTAGGG GCGGGATGGG 
     7681 CGGAGTTAGG GGCGGGACTA TGGTTGCTGA CTAATTGAGA TGCATGCTTT GCATACTTCT 
     7741 GCCTGCTGGG GAGCCTGGGG ACTTTCCACA CCTGGTTGCT GACTAATTGA GATGCATGCT 
     7801 TTGCATACTT CTGCCTGCTG GGGAGCCTGG GGACTTTCCA CACCCTAACT GACACACATT 
     7861 CCACAGCTGG TTCTTTCCGC CTCAGGACTC TTCCTTTTTC AATATTATTG AAGCATTTAT 
     7921 CAGGGTTATT GTCTCATGAG CGGATACATA TTTGAATGTA TTTAGAAAAA TAAACAAATA 
     7981 GGGGTTCCGC GCACATTTCC CCGAAAAGTG CCACCTGACG CGCCCTGTAG CGGCGCATTA 
     8041 AGCGCGGCGG GTGTGGTGGT TACGCGCAGC GTGACCGCTA CACTTGCCAG CGCCCTAGCG 
     8101 CCCGCTCCTT TCGCTTTCTT CCCTTCCTTT CTCGCCACGT TCGCCGGCTT TCCCCGTCAA 
     8161 GCTCTAAATC GGGGGCTCCC TTTAGGGTTC CGATTTAGTG CTTTACGGCA CCTCGACCCC 
     8221 AAAAAACTTG ATTAGGGTGA TGGTTCACGT AGTGGGCCAT CGCCCTGATA GACGGTTTTT 
     8281 CGCCCTTTGA CGTTGGAGTC CACGTTCTTT AATAGTGGAC TCTTGTTCCA AACTGGAACA 
     8341 ACACTCAACC CTATCTCGGT CTATTCTTTT GATTTATAAG GGATTTTGCC GATTTCGGCC 
     8401 TATTGGTTAA AAAATGAGCT GATTTAACAA AAATTTAACG CGAATTTT
//