LOCUS RC213277L4 9497 bp DNA circular SYN DEFINITION ESR1 (Myc-DDK-tagged)-Human estrogen receptor 1 (ESR1), transcript variant 1 ACCESSION NM_000125 VERSION null SOURCE synthetic construct FEATURES Location/Qualifiers misc_feature 7788..7968 /label="3-LTR" misc_feature 833..1013 /label="5-LTR" misc_feature 8696..9342 /label="CAT(Cam-r)" misc_feature 2620..3234 /label="CMV" misc_feature 6663..6683 /label="LR50" misc_feature 5126..5854 /label="mGFP" misc_feature 5861..5926 /label="P2A" misc_feature 8016..8635 /label="pBR322 Ori" misc_feature 5927..6526 /label="Puro" misc_feature 3252..3271 /label="T7" misc_feature 3221..3237 /label="V2" misc_feature 3320..3328 /label="Restriction Enzyme" misc_feature 5105..5111 /label="Restriction Enzyme" misc_feature 3320..5105 /label="ESR1" ORIGIN 1 GTCGACGGAT CGGGAGATCT CCCGATCCCC TATGGTGCAC TCTCAGTACA ATCTGCTCTG 61 ATGCCGCATA GTTAAGCCAG TATCTGCTCC CTGCTTGTGT GTTGGAGGTC GCTGAGTAGT 121 GCGCGAGCAA AATTTAAGCT ACAACAAGGC AAGGCTTGAC CGACAATTGC ATGAAGAATC 181 TGCTTAGGGT TAGGCGTTTT GCGCTGCTTC GCGATGTACG GGCCAGATAT CGCGTTGACA 241 TTGATTATTG ACTAGTTATT AATAGTAATC AATTACGGGG TCATTAGTTC ATAGCCCATA 301 TATGGAGTTC CGCGTTACAT AACTTACGGT AAATGGCCCG CCTGGCTGAC CGCCCAACGA 361 CCCCCGCCCA TTGACGTCAA TAATGACGTA TGTTCCCATA GTAACGCCAA TAGGGACTTT 421 CCATTGACGT CAATGGGTGG AGTATTTACG GTAAACTGCC CACTTGGCAG TACATCAAGT 481 GTATCATATG CCAAGTACGC CCCCTATTGA CGTCAATGAC GGTAAATGGC CCGCCTGGCA 541 TTATGCCCAG TACATGACCT TATGGGACTT TCCTACTTGG CAGTACATCT ACGTATTAGT 601 CATCGCTATT ACCATGGTGA TGCGGTTTTG GCAGTACATC AATGGGCGTG GATAGCGGTT 661 TGACTCACGG GGATTTCCAA GTCTCCACCC CATTGACGTC AATGGGAGTT TGTTTTGGCA 721 CCAAAATCAA CGGGACTTTC CAAAATGTCG TAACAACTCC GCCCCATTGA CGCAAATGGG 781 CGGTAGGCGT GTACGGTGGG AGGTCTATAT AAGCAGCGCG TTTTGCCTGT ACTGGGTCTC 841 TCTGGTTAGA CCAGATCTGA GCCTGGGAGC TCTCTGGCTA ACTAGGGAAC CCACTGCTTA 901 AGCCTCAATA AAGCTTGCCT TGAGTGCTTC AAGTAGTGTG TGCCCGTCTG TTGTGTGACT 961 CTGGTAACTA GAGATCCCTC AGACCCTTTT AGTCAGTGTG GAAAATCTCT AGCAGTGGCG 1021 CCCGAACAGG GACTTGAAAG CGAAAGGGAA ACCAGAGGAG CTCTCTCGAC GCAGGACTCG 1081 GCTTGCTGAA GCGCGCACGG CAAGAGGCGA GGGGCGGCGA CTGGTGAGTA CGCCAAAAAT 1141 TTTGACTAGC GGAGGCTAGA AGGAGAGAGA TGGGTGCGAG AGCGTCAGTA TTAAGCGGGG 1201 GAGAATTAGA TCGCGATGGG AAAAAATTCG GTTAAGGCCA GGGGGAAAGA AAAAATATAA 1261 ATTAAAACAT ATAGTATGGG CAAGCAGGGA GCTAGAACGA TTCGCAGTTA ATCCTGGCCT 1321 GTTAGAAACA TCAGAAGGCT GTAGACAAAT ACTGGGACAG CTACAACCAT CCCTTCAGAC 1381 AGGATCAGAA GAACTTAGAT CATTATATAA TACAGTAGCA ACCCTCTATT GTGTGCATCA 1441 AAGGATAGAG ATAAAAGACA CCAAGGAAGC TTTAGACAAG ATAGAGGAAG AGCAAAACAA 1501 AAGTAAGACC ACCGCACAGC AAGCGGCCGG CCGCTGATCT TCAGACCTGG AGGAGGAGAT 1561 ATGAGGGACA ATTGGAGAAG TGAATTATAT AAATATAAAG TAGTAAAAAT TGAACCATTA 1621 GGAGTAGCAC CCACCAAGGC AAAGAGAAGA GTGGTGCAGA GAGAAAAAAG AGCAGTGGGA 1681 ATAGGAGCTT TGTTCCTTGG GTTCTTGGGA GCAGCAGGAA GCACTATGGG CGCAGCGTCA 1741 ATGACGCTGA CGGTACAGGC CAGACAATTA TTGTCTGGTA TAGTGCAGCA GCAGAACAAT 1801 TTGCTGAGGG CTATTGAGGC GCAACAGCAT CTGTTGCAAC TCACAGTCTG GGGCATCAAG 1861 CAGCTCCAGG CAAGAATCCT GGCTGTGGAA AGATACCTAA AGGATCAACA GCTCCTGGGG 1921 ATTTGGGGTT GCTCTGGAAA ACTCATTTGC ACCACTGCTG TGCCTTGGAA TGCTAGTTGG 1981 AGTAATAAAT CTCTGGAACA GATTTGGAAT CACACGACCT GGATGGAGTG GGACAGAGAA 2041 ATTAACAATT ACACAAGCTT AATACACTCC TTAATTGAAG AATCGCAAAA CCAGCAAGAA 2101 AAGAATGAAC AAGAATTATT GGAATTAGAT AAATGGGCAA GTTTGTGGAA TTGGTTTAAC 2161 ATAACAAATT GGCTGTGGTA TATAAAATTA TTCATAATGA TAGTAGGAGG CTTGGTAGGT 2221 TTAAGAATAG TTTTTGCTGT ACTTTCTATA GTGAATAGAG TTAGGCAGGG ATATTCACCA 2281 TTATCGTTTC AGACCCACCT CCCAACCCCG AGGGGACCCG ACAGGCCCGA AGGAATAGAA 2341 GAAGAAGGTG GAGAGAGAGA CAGAGACAGA TCCATTCGAT TAGTGAACGG ATCGGCACTG 2401 CGTGCGCCAA TTCTGCAGAC AAATGGCAGT ATTCATCCAC AATTTTAAAA GAAAAGGGGG 2461 GATTGGGGGG TACAGTGCAG GGGAAAGAAT AGTAGACATA ATAGCAACAG ACATACAAAC 2521 TAAAGAATTA CAAAAACAAA TTACAAAAAT TCAAAATTTT CGGGTTTATT ACAGGGACAG 2581 CAGAGATCCA GTTTGGTTAG TACCGGGCCC GCTCTAGACA TGTCCAATAT GACCGCCATG 2641 TTGACATTGA TTATTGACTA GTTATTAATA GTAATCAATT ACGGGGTCAT TAGTTCATAG 2701 CCCATATATG GAGTTCCGCG TTACATAACT TACGGTAAAT GGCCCGCCTG GCTGACCGCC 2761 CAACGACCCC CGCCCATTGA CGTCAATAAT GACGTATGTT CCCATAGTAA CGCCAATAGG 2821 GACTTTCCAT TGACGTCAAT GGGTGGAGTA TTTACGGTAA ACTGCCCACT TGGCAGTACA 2881 TCAAGTGTAT CATATGCCAA GTCCGCCCCC TATTGACGTC AATGACGGTA AATGGCCCGC 2941 CTGGCATTAT GCCCAGTACA TGACCTTACG GGACTTTCCT ACTTGGCAGT ACATCTACGT 3001 ATTAGTCATC GCTATTACCA TGGTGATGCG GTTTTGGCAG TACACCAATG GGCGTGGATA 3061 GCGGTTTGAC TCACGGGGAT TTCCAAGTCT CCACCCCATT GACGTCAATG GGAGTTTGTT 3121 TTGGCACCAA AATCAACGGG ACTTTCCAAA ATGTCGTAAT AACCCCGCCC CGTTGACGCA 3181 AATGGGCGGT AGGCGTGTAC GGTGGGAGGT CTATATAAGC AGAGCTCGTT TAGTGAACCG 3241 TCAGAATTTT GTAATACGAC TCACTATAGG GCGGCCGGGA ATTCGTCGAC TGGATCCGGT 3301 ACCGAGGAGA TCTGCCGCCG CGATCGCCAT GACCATGACC CTCCACACCA AAGCATCTGG 3361 GATGGCCCTA CTGCATCAGA TCCAAGGGAA CGAGCTGGAG CCCCTGAACC GTCCGCAGCT 3421 CAAGATCCCC CTGGAGCGGC CCCTGGGCGA GGTGTACCTG GACAGCAGCA AGCCCGCCGT 3481 GTACAACTAC CCCGAGGGCG CCGCCTACGA GTTCAACGCC GCGGCCGCCG CCAACGCGCA 3541 GGTCTACGGT CAGACCGGCC TCCCCTACGG CCCCGGGTCT GAGGCTGCGG CGTTCGGCTC 3601 CAACGGCCTG GGGGGTTTCC CCCCACTCAA CAGCGTGTCT CCGAGCCCGC TGATGCTACT 3661 GCACCCGCCG CCGCAGCTGT CGCCTTTCCT GCAGCCCCAC GGCCAGCAGG TGCCCTACTA 3721 CCTGGAGAAC GAGCCCAGCG GCTACACGGT GCGCGAGGCC GGCCCGCCGG CATTCTACAG 3781 GCCAAATTCA GATAATCGAC GCCAGGGTGG CAGAGAAAGA TTGGCCAGTA CCAATGACAA 3841 GGGAAGTATG GCTATGGAAT CTGCCAAGGA GACTCGCTAC TGTGCAGTGT GCAATGACTA 3901 TGCTTCAGGC TACCATTATG GAGTCTGGTC CTGTGAGGGC TGCAAGGCCT TCTTCAAGAG 3961 AAGTATTCAA GGACATAACG ACTATATGTG TCCAGCCACC AACCAGTGCA CCATTGATAA 4021 AAACAGGAGG AAGAGCTGCC AGGCCTGCCG GCTCCGCAAA TGCTACGAAG TGGGAATGAT 4081 GAAAGGTGGG ATACGAAAAG ACCGAAGAGG AGGGAGAATG TTGAAACACA AGCGCCAGAG 4141 AGATGATGGG GAGGGCAGGG GTGAAGTGGG GTCTGCTGGA GACATGAGAG CTGCCAACCT 4201 TTGGCCAAGC CCGCTCATGA TCAAACGCTC TAAGAAGAAC AGCCTGGCCT TGTCCCTGAC 4261 GGCCGACCAG ATGGTCAGTG CCTTGTTGGA TGCTGAGCCC CCGATACTCT ATTCCGAGTA 4321 TGATCCTACC AGACCCTTCA GTGAAGCTTC GATGATGGGC TTACTGACCA ACCTGGCAGA 4381 CAGGGAGCTG GTTCACATGA TCAACTGGGC GAAGAGGGTG CCAGGCTTTG TGGATTTGAC 4441 CCTCCATGAT CAGGTCCACC TTCTAGAATG TGCCTGGCTA GAGATCCTGA TGATTGGTCT 4501 CGTCTGGCGC TCCATGGAGC ACCCAGGGAA GCTACTGTTT GCTCCTAACT TGCTCTTGGA 4561 CAGGAACCAG GGAAAATGTG TAGAGGGCAT GGTGGAGATC TTCGACATGC TGCTGGCTAC 4621 ATCATCTCGG TTCCGCATGA TGAATCTGCA GGGAGAGGAG TTTGTGTGCC TCAAATCTAT 4681 TATTTTGCTT AATTCTGGAG TGTACACATT TCTGTCCAGC ACCCTGAAGT CTCTGGAAGA 4741 GAAGGACCAT ATCCACCGAG TCCTGGACAA GATCACAGAC ACTTTGATCC ACCTGATGGC 4801 CAAGGCAGGC CTGACCCTGC AGCAGCAGCA CCAGCGGCTG GCCCAGCTCC TCCTCATCCT 4861 CTCCCACATC AGGCACATGA GTAACAAAGG CATGGAGCAT CTGTACAGCA TGAAGTGCAA 4921 GAACGTGGTG CCCCTCTATG ACCTGCTGCT GGAGATGCTG GACGCCCACC GCCTACATGC 4981 GCCCACTAGC CGTGGAGGGG CATCCGTGGA GGAGACGGAC CAAAGCCACT TGGCCACTGC 5041 GGGCTCTACT TCATCGCATT CCTTGCAAAA GTATTACATC ACGGGGGAGG CAGAGGGTTT 5101 CCCTGCCACG GTCACGCGTA CGCGGCCGCT CGAGATGAGC GGGGGCGAGG AGCTGTTCGC 5161 CGGCATCGTG CCCGTGCTGA TCGAGCTGGA CGGCGACGTG CACGGCCACA AGTTCAGCGT 5221 GCGCGGCGAG GGCGAGGGCG ACGCCGACTA CGGCAAGCTG GAGATCAAGT TCATCTGCAC 5281 CACCGGCAAG CTGCCCGTGC CCTGGCCCAC CCTGGTGACC ACCCTCTGCT ACGGCATCCA 5341 GTGCTTCGCC CGCTACCCCG AGCACATGAA GATGAACGAC TTCTTCAAGA GCGCCATGCC 5401 CGAGGGCTAC ATCCAGGAGC GCACCATCCA GTTCCAGGAC GACGGCAAGT ACAAGACCCG 5461 CGGCGAGGTG AAGTTCGAGG GCGACACCCT GGTGAACCGC ATCGAGCTGA AGGGCAAGGA 5521 CTTCAAGGAG GACGGCAACA TCCTGGGCCA CAAGCTGGAG TACAGCTTCA ACAGCCACAA 5581 CGTGTACATC CGCCCCGACA AGGCCAACAA CGGCCTGGAG GCTAACTTCA AGACCCGCCA 5641 CAACATCGAG GGCGGCGGCG TGCAGCTGGC CGACCACTAC CAGACCAACG TGCCCCTGGG 5701 CGACGGCCCC GTGCTGATCC CCATCAACCA CTACCTGAGC ACTCAGACCA AGATCAGCAA 5761 GGACCGCAAC GAGGCCCGCG ACCACATGGT GCTCCTGGAG TCCTTCAGCG CCTGCTGCCA 5821 CACCCACGGC ATGGACGAGC TGTACAGGTC CGGACTCAGA GTTTGGGTAG GAAGCGGAGC 5881 TACTAACTTC AGCCTGCTGA AGCAGGCTGG AGACGTGGAG GAGAACCCTG GACCTATGAC 5941 CGAGTACAAG CCCACGGTGC GCCTCGCCAC CCGCGACGAC GTCCCCAGGG CCGTACGCAC 6001 CCTCGCCGCC GCGTTCGCCG ACTACCCCGC CACGCGCCAC ACCGTCGATC CGGATCGCCA 6061 CATCGAGCGG GTCACCGAGC TGCAAGAACT CTTCCTCACG CGCGTCGGGC TCGACATCGG 6121 CAAGGTGTGG GTCGCGGACG ACGGCGCCGC GGTGGCGGTC TGGACCACGC CGGAGAGCGT 6181 CGAAGCGGGG GCGGTGTTCG CCGAGATCGG CCCGCGCATG GCCGAGTTGA GCGGTTCCCG 6241 GCTGGCCGCG CAGCAACAGA TGGAAGGCCT CCTGGCGCCG CACCGGCCCA AGGAGCCCGC 6301 GTGGTTCCTG GCCACCGTCG GCGTCTCGCC CGACCACCAG GGCAAGGGTC TGGGCAGCGC 6361 CGTCGTGCTC CCCGGAGTGG AGGCGGCCGA GCGCGCCGGG GTGCCCGCCT TCCTGGAGAC 6421 CTCCGCGCCC CGCAACCTCC CCTTCTACGA GCGGCTCGGC TTCACCGTCA CCGCCGACGT 6481 CGAGGTGCCC GAAGGACCGC GCACCTGGTG CATGACCCGC AAGCCCGGTG CCTGATGTAC 6541 ACCCAAACGG CCGGCCGCGG TCTGTACAAG TAGGATTCGT CGAGGGACCT AATAACTTCG 6601 TATAGCATAC ATTATACGAA GTTATACATG TTTAAGGGTT CCGGTTCCAC TAGGTACAAT 6661 TCGATATCAA GCTTATCGAT AATCAACCTC TGGATTACAA AATTTGTGAA AGATTGACTG 6721 GTATTCTTAA CTATGTTGCT CCTTTTACGC TATGTGGATA CGCTGCTTTA ATGCCTTTGT 6781 ATCATGCTAT TGCTTCCCGT ATGGCTTTCA TTTTCTCCTC CTTGTATAAA TCCTGGTTGC 6841 TGTCTCTTTA TGAGGAGTTG TGGCCCGTTG TCAGGCAACG TGGCGTGGTG TGCACTGTGT 6901 TTGCTGACGC AACCCCCACT GGTTGGGGCA TTGCCACCAC CTGTCAGCTC CTTTCCGGGA 6961 CTTTCGCTTT CCCCCTCCCT ATTGCCACGG CGGAACTCAT CGCCGCCTGC CTTGCCCGCT 7021 GCTGGACAGG GGCTCGGCTG TTGGGCACTG ACAATTCCGT GGTGTTGTCG GGGAAATCAT 7081 CGTCCTTTCC TTGGCTGCTC GCCTGTGTTG CCACCTGGAT TCTGCGCGGG ACGTCCTTCT 7141 GCTACGTCCC TTCGGCCCTC AATCCAGCGG ACCTTCCTTC CCGCGGCCTG CTGCCGGCTC 7201 TGCGGCCTCT TCCGCGTCTT CGCCTTCGCC CTCAGACGAG TCGGATCTCC CTTTGGGCCG 7261 CCTCCCCGCA TCGATACCGT CGACCTCGAT CGAGACCTAG AAAAACATGG AGCAATCACA 7321 AGTAGCAATA CAGCAGCTAC CAATGCTGAT TGTGCCTGGC TAGAAGCACA AGAGGAGGAG 7381 GAGGTGGGTT TTCCAGTCAC ACCTCAGGTA CCTTTAAGAC CAATGACTTA CAAGGCAGCT 7441 GTAGATCTTA GCCACTTTTT AAAAGAAAAG GGGGGACTGG AAGGGCTAAT TCACTCCCAA 7501 CGAAGACAAG ATATCCTTGA TCTGTGGATC TACCACACAC AAGGCTACTT CCCTGATTGG 7561 CAGAACTACA CACCAGGGCC AGGGATCAGA TATCCACTGA CCTTTGGATG GTGCTACAAG 7621 CTAGTACCAG TTGAGCAAGA GAAGGTAGAA GAAGCCAATG AAGGAGAGAA CACCCGCTTG 7681 TTACACCCTG TGAGCCTGCA TGGGATGGAT GACCCGGAGA GAGAAGTATT AGAGTGGAGG 7741 TTTGACAGCC GCCTAGCATT TCATCACATG GCCCGAGAGC TGCATCCGGA CTGTACTGGG 7801 TCTCTCTGGT TAGACCAGAT CTGAGCCTGG GAGCTCTCTG GCTAACTAGG GAACCCACTG 7861 CTTAAGCCTC AATAAAGCTT GCCTTGAGTG CTTCAAGTAG TGTGTGCCCG TCTGTTGTGT 7921 GACTCTGGTA ACTAGAGATC CCTCAGACCC TTTTAGTCAG TGTGGAAAAT CTCTAGCAGC 7981 ATGTGAGCAA AAGGCCAGCA AAAGGCCAGG AACCGTAAAA AGGCCGCGTT GCTGGCGTTT 8041 TTCCATAGGC TCCGCCCCCC TGACGAGCAT CACAAAAATC GACGCTCAAG TCAGAGGTGG 8101 CGAAACCCGA CAGGACTATA AAGATACCAG GCGTTTCCCC CTGGAAGCTC CCTCGTGCGC 8161 TCTCCTGTTC CGACCCTGCC GCTTACCGGA TACCTGTCCG CCTTTCTCCC TTCGGGAAGC 8221 GTGGCGCTTT CTCATAGCTC ACGCTGTAGG TATCTCAGTT CGGTGTAGGT CGTTCGCTCC 8281 AAGCTGGGCT GTGTGCACGA ACCCCCCGTT CAGCCCGACC GCTGCGCCTT ATCCGGTAAC 8341 TATCGTCTTG AGTCCAACCC GGTAAGACAC GACTTATCGC CACTGGCAGC AGCCACTGGT 8401 AACAGGATTA GCAGAGCGAG GTATGTAGGC GGTGCTACAG AGTTCTTGAA GTGGTGGCCT 8461 AACTACGGCT ACACTAGAAG AACAGTATTT GGTATCTGCG CTCTGCTGAA GCCAGTTACC 8521 TTCGGAAAAA GAGTTGGTAG CTCTTGATCC GGCAAACAAA CCACCGCTGG TAGCGGTGGT 8581 TTTTTTGTTT GCAAGCAGCA GATTACGCGC AGAAAAAAAG GATCTCAAGA AGATCCTTTG 8641 ATCTTTTCTA CGGGGTCTGA CGCTCAGTGG AACGAAAACT CACGTTAAGG GATTTTGGTC 8701 ATGATTACGC CCCGCCCTGC CACTCATCGC AGTACTGTTG TAATTCATTA AGCATTCTGC 8761 CGACATGGAA GCCATCACAA ACGGCATGAT GAACCTGAAT CGCCAGCGGC ATCAGCACCT 8821 TGTCGCCTTG CGTATAATAT TTGCCCATGG TGAAAACGGG GGCGAAGAAG TTGTCCATAT 8881 TGGCCACGTT TAAATCAAAA CTGGTGAAAC TCACCCAGGG ATTGGCTGAG ACGAAAAACA 8941 TATTCTCAAT AAACCCTTTA GGGAAATAGG CCAGGTTTTC ACCGTAACAC GCCACATCTT 9001 GCGAATATAT GTGTAGAAAC TGCCGGAAAT CGTCGTGGTA TTCACTCCAG AGCGATGAAA 9061 ACGTTTCAGT TTGCTCATGG AAAACGGTGT AACAAGGGTG AACACTATCC CATATCACCA 9121 GCTCACCGTC TTTCATTGCC ATACGGAACT CCGGATGAGC ATTCATCAGG CGGGCAAGAA 9181 TGTGAATAAA GGCCGGATAA AACTTGTGCT TATTTTTCTT TACGGTCTTT AAAAAGGCCG 9241 TAATATCCAG CTGAACGGTC TGGTTATAGG TACATTGAGC AACTGACTGA AATGCCTCAA 9301 AATGTTCTTT ACGATGCCAT TGGGATATAT CAACGGTGGT ATATCCAGTG ATTTTTTTCT 9361 CCATACTCTT CCTTTTTCAA TATTATTGAA GCATTTATCA GGGTTATTGT CTCATGAGCG 9421 GATACATATT TGAATGTATT TAGAAAAATA AACAAATAGG GGTTCCGCGC ACATTTCCCC 9481 GAAAAGTGCC ACCTGAC //