LOCUS RC213277 6666 bp DNA circular SYN DEFINITION ESR1 (Myc-DDK-tagged)-Human estrogen receptor 1 (ESR1), transcript variant 1 ACCESSION NM_000125 VERSION null SOURCE synthetic construct FEATURES Location/Qualifiers misc_feature 201..924 /label="CMV promoter" misc_feature 839..856 /label="Restriction Enzyme" misc_feature 953..971 /label="T7 promoter" misc_feature 2823..2914 /label="Myc-DDK" misc_feature 2944..3529 /label="PolyA signal" misc_feature 3001..3020 /label="Restriction Enzyme" misc_feature 3805..4477 /label="ColE1" misc_feature 4907..5701 /label="Kan/Neo" misc_feature 5736..6038 /label="SV40 ori" misc_feature 6124..6601 /label="f1 ori" misc_feature 1020..1028 /label="Restriction Enzyme" misc_feature 2805..2811 /label="Restriction Enzyme" misc_feature 1020..2805 /label="ESR1" ORIGIN 1 AACAAAATAT TAACGCTTAC AATTTCCATT CGCCATTCAG GCTGCGCAAC TGTTGGGAAG 61 GGCGATCGGT GCGGGCCTCT TCGCTATTAC GCCAGCTGGC GAAAGGGGGA TGTGCTGCAA 121 GGCGATTAAG TTGGGTAACG CCAGGGTTTT CCCAGTCACG ACGTTGTAAA ACGACGGCCA 181 GTGCCAAGCT GATCTATACA TTGAATCAAT ATTGGCAATT AGCCATATTA GTCATTGGTT 241 ATATAGCATA AATCAATATT GGCTATTGGC CATTGCATAC GTTGTATCTA TATCATAATA 301 TGTACATTTA TATTGGCTCA TGTCCAATAT GACCGCCATG TTGACATTGA TTATTGACTA 361 GTTATTAATA GTAATCAATT ACGGGGTCAT TAGTTCATAG CCCATATATG GAGTTCCGCG 421 TTACATAACT TACGGTAAAT GGCCCGCCTG GCTGACCGCC CAACGACCCC CGCCCATTGA 481 CGTCAATAAT GACGTATGTT CCCATAGTAA CGCCAATAGG GACTTTCCAT TGACGTCAAT 541 GGGTGGAGTA TTTACGGTAA ACTGCCCACT TGGCAGTACA TCAAGTGTAT CATATGCCAA 601 GTCCGCCCCC TATTGACGTC AATGACGGTA AATGGCCCGC CTGGCATTAT GCCCAGTACA 661 TGACCTTACG GGACTTTCCT ACTTGGCAGT ACATCTACGT ATTAGTCATC GCTATTACCA 721 TGGTGATGCG GTTTTGGCAG TACACCAATG GGCGTGGATA GCGGTTTGAC TCACGGGGAT 781 TTCCAAGTCT CCACCCCATT GACGTCAATG GGAGTTTGTT TTGGCACCAA AATCAACGGG 841 ACTTTCCAAA ATGTCGTAAT AACCCCGCCC CGTTGACGCA AATGGGCGGT AGGCGTGTAC 901 GGTGGGAGGT CTATATAAGC AGAGCTCGTT TAGTGAACCG TCAGAATTTT GTAATACGAC 961 TCACTATAGG GCGGCCGGGA ATTCGTCGAC TGGATCCGGT ACCGAGGAGA TCTGCCGCCG 1021 CGATCGCCAT GACCATGACC CTCCACACCA AAGCATCTGG GATGGCCCTA CTGCATCAGA 1081 TCCAAGGGAA CGAGCTGGAG CCCCTGAACC GTCCGCAGCT CAAGATCCCC CTGGAGCGGC 1141 CCCTGGGCGA GGTGTACCTG GACAGCAGCA AGCCCGCCGT GTACAACTAC CCCGAGGGCG 1201 CCGCCTACGA GTTCAACGCC GCGGCCGCCG CCAACGCGCA GGTCTACGGT CAGACCGGCC 1261 TCCCCTACGG CCCCGGGTCT GAGGCTGCGG CGTTCGGCTC CAACGGCCTG GGGGGTTTCC 1321 CCCCACTCAA CAGCGTGTCT CCGAGCCCGC TGATGCTACT GCACCCGCCG CCGCAGCTGT 1381 CGCCTTTCCT GCAGCCCCAC GGCCAGCAGG TGCCCTACTA CCTGGAGAAC GAGCCCAGCG 1441 GCTACACGGT GCGCGAGGCC GGCCCGCCGG CATTCTACAG GCCAAATTCA GATAATCGAC 1501 GCCAGGGTGG CAGAGAAAGA TTGGCCAGTA CCAATGACAA GGGAAGTATG GCTATGGAAT 1561 CTGCCAAGGA GACTCGCTAC TGTGCAGTGT GCAATGACTA TGCTTCAGGC TACCATTATG 1621 GAGTCTGGTC CTGTGAGGGC TGCAAGGCCT TCTTCAAGAG AAGTATTCAA GGACATAACG 1681 ACTATATGTG TCCAGCCACC AACCAGTGCA CCATTGATAA AAACAGGAGG AAGAGCTGCC 1741 AGGCCTGCCG GCTCCGCAAA TGCTACGAAG TGGGAATGAT GAAAGGTGGG ATACGAAAAG 1801 ACCGAAGAGG AGGGAGAATG TTGAAACACA AGCGCCAGAG AGATGATGGG GAGGGCAGGG 1861 GTGAAGTGGG GTCTGCTGGA GACATGAGAG CTGCCAACCT TTGGCCAAGC CCGCTCATGA 1921 TCAAACGCTC TAAGAAGAAC AGCCTGGCCT TGTCCCTGAC GGCCGACCAG ATGGTCAGTG 1981 CCTTGTTGGA TGCTGAGCCC CCGATACTCT ATTCCGAGTA TGATCCTACC AGACCCTTCA 2041 GTGAAGCTTC GATGATGGGC TTACTGACCA ACCTGGCAGA CAGGGAGCTG GTTCACATGA 2101 TCAACTGGGC GAAGAGGGTG CCAGGCTTTG TGGATTTGAC CCTCCATGAT CAGGTCCACC 2161 TTCTAGAATG TGCCTGGCTA GAGATCCTGA TGATTGGTCT CGTCTGGCGC TCCATGGAGC 2221 ACCCAGGGAA GCTACTGTTT GCTCCTAACT TGCTCTTGGA CAGGAACCAG GGAAAATGTG 2281 TAGAGGGCAT GGTGGAGATC TTCGACATGC TGCTGGCTAC ATCATCTCGG TTCCGCATGA 2341 TGAATCTGCA GGGAGAGGAG TTTGTGTGCC TCAAATCTAT TATTTTGCTT AATTCTGGAG 2401 TGTACACATT TCTGTCCAGC ACCCTGAAGT CTCTGGAAGA GAAGGACCAT ATCCACCGAG 2461 TCCTGGACAA GATCACAGAC ACTTTGATCC ACCTGATGGC CAAGGCAGGC CTGACCCTGC 2521 AGCAGCAGCA CCAGCGGCTG GCCCAGCTCC TCCTCATCCT CTCCCACATC AGGCACATGA 2581 GTAACAAAGG CATGGAGCAT CTGTACAGCA TGAAGTGCAA GAACGTGGTG CCCCTCTATG 2641 ACCTGCTGCT GGAGATGCTG GACGCCCACC GCCTACATGC GCCCACTAGC CGTGGAGGGG 2701 CATCCGTGGA GGAGACGGAC CAAAGCCACT TGGCCACTGC GGGCTCTACT TCATCGCATT 2761 CCTTGCAAAA GTATTACATC ACGGGGGAGG CAGAGGGTTT CCCTGCCACG GTCACGCGTA 2821 CGCGGCCGCT CGAGCAGAAA CTCATCTCAG AAGAGGATCT GGCAGCAAAT GATATCCTGG 2881 ATTACAAGGA TGACGACGAT AAGGTTTAAA CGGCCGGCCG CGGTCATAGC TGTTTCCTGA 2941 ACAGATCCCG GGTGGCATCC CTGTGACCCC TCCCCAGTGC CTCTCCTGGC CCTGGAAGTT 3001 GCCACTCCAG TGCCCACCAG CCTTGTCCTA ATAAAATTAA GTTGCATCAT TTTGTCTGAC 3061 TAGGTGTCCT TCTATAATAT TATGGGGTGG AGGGGGGTGG TATGGAGCAA GGGGCAAGTT 3121 GGGAAGACAA CCTGTAGGGC CTGCGGGGTC TATTGGGAAC CAAGCTGGAG TGCAGTGGCA 3181 CAATCTTGGC TCACTGCAAT CTCCGCCTCC TGGGTTCAAG CGATTCTCCT GCCTCAGCCT 3241 CCCGAGTTGT TGGGATTCCA GGCATGCATG ACCAGGCTCA GCTAATTTTT GTTTTTTTGG 3301 TAGAGACGGG GTTTCACCAT ATTGGCCAGG CTGGTCTCCA ACTCCTAATC TCAGGTGATC 3361 TACCCACCTT GGCCTCCCAA ATTGCTGGGA TTACAGGCGT GAACCACTGC TCCCTTCCCT 3421 GTCCTTCTGA TTTTAAAATA ACTATACCAG CAGGAGGACG TCCAGACACA GCATAGGCTA 3481 CCTGGCCATG CCCAACCGGT GGGACATTTG AGTTGCTTGC TTGGCACTGT CCTCTCATGC 3541 GTTGGGTCCA CTCAGTAGAT GCCTGTTGAA TTGGGTACGC GGCCAGCGGC GAGCGGTATC 3601 AGCTCACTCA AAGGCGGTAA TACGGTTATC CACAGAATCA GGGGATAACG CAGGAAAGAA 3661 CATGTGAGCA AAAGGCCAGC AAAAGGCCAG GAACCGTAAA AAGGCCGCGT TGCTGGCGTT 3721 TTTCCATAGG CTCCGCCCCC CTGACGAGCA TCACAAAAAT CGACGCTCAA GTCAGAGGTG 3781 GCGAAACCCG ACAGGACTAT AAAGATACCA GGCGTTTCCC CCTGGAAGCT CCCTCGTGCG 3841 CTCTCCTGTT CCGACCCTGC CGCTTACCGG ATACCTGTCC GCCTTTCTCC CTTCGGGAAG 3901 CGTGGCGCTT TCTCATAGCT CACGCTGTAG GTATCTCAGT TCGGTGTAGG TCGTTCGCTC 3961 CAAGCTGGGC TGTGTGCACG AACCCCCCGT TCAGCCCGAC CGCTGCGCCT TATCCGGTAA 4021 CTATCGTCTT GAGTCCAACC CGGTAAGACA CGACTTATCG CCACTGGCAG CAGCCACTGG 4081 TAACAGGATT AGCAGAGCGA GGTATGTAGG CGGTGCTACA GAGTTCTTGA AGTGGTGGCC 4141 TAACTACGGC TACACTAGAA GAACAGTATT TGGTATCTGC GCTCTGCTGA AGCCAGTTAC 4201 CTTCGGAAAA AGAGTTGGTA GCTCTTGATC CGGCAAACAA ACCACCGCTG GTAGCGGTGG 4261 TTTTTTTGTT TGCAAGCAGC AGATTACGCG CAGAAAAAAA GGATCTCAAG AAGATCCTTT 4321 GATCTTTTCT ACGGGGTCTG ACGCTCAGTG GAACGAAAAC TCACGTTAAG GGATTTTGGT 4381 CATGAGATTA TCAAAAAGGA TCTTCACCTA GATCCTTTTA AATTAAAAAT GAAGTTTTAA 4441 ATCAATCTAA AGTATATATG AGTAACCTGA GGCTATGGCA GGGCCTGCCG CCCCGACGTT 4501 GGCTGCGAGC CCTGGGCCTT CACCCGAACT TGGGGGGTGG GGTGGGGAAA AGGAAGAAAC 4561 GCGGGCGTAT TGGCCCCAAT GGGGTCTCGG TGGGGTATCG ACAGAGTGCC AGCCCTGGGA 4621 CCGAACCCCG CGTTTATGAA CAAACGACCC AACACCGTGC GTTTTATTCT GTCTTTTTAT 4681 TGCCGTCATA GCGCGGGTTC CTTCCGGTAT TGTCTCCTTC CGTGTTTCAG TTAGCCTCCC 4741 CCTAGGGTGG GCGAAGAACT CCAGCATGAG ATCCCCGCGC TGGAGGATCA TCCAGCCGGC 4801 GTCCCGGAAA ACGATTCCGA AGCCCAACCT TTCATAGAAG GCGGCGGTGG AATCGAAATC 4861 TCGTGATGGC AGGTTGGGCG TCGCTTGGTC GGTCATTTCG AACCCCAGAG TCCCGCTCAG 4921 AAGAACTCGT CAAGAAGGCG ATAGAAGGCG ATGCGCTGCG AATCGGGAGC GGCGATACCG 4981 TAAAGCACGA GGAAGCGGTC AGCCCATTCG CCGCCAAGCT CTTCAGCAAT ATCACGGGTA 5041 GCCAACGCTA TGTCCTGATA GCGATCCGCC ACACCCAGCC GGCCACAGTC GATGAATCCA 5101 GAAAAGCGGC CATTTTCCAC CATGATATTC GGCAAGCAGG CATCGCCATG GGTCACGACG 5161 AGATCCTCGC CGTCGGGCAT GCTCGCCTTG AGCCTGGCGA ACAGTTCGGC TGGCGCGAGC 5221 CCCTGATGCT CTTCGTCCAG ATCATCCTGA TCGACAAGAC CGGCTTCCAT CCGAGTACGT 5281 GCTCGCTCGA TGCGATGTTT CGCTTGGTGG TCGAATGGGC AGGTAGCCGG ATCAAGCGTA 5341 TGCAGCCGCC GCATTGCATC AGCCATGATG GATACTTTCT CGGCAGGAGC AAGGTGAGAT 5401 GACAGGAGAT CCTGCCCCGG CACTTCGCCC AATAGCAGCC AGTCCCTTCC CGCTTCAGTG 5461 ACAACGTCGA GCACAGCTGC GCAAGGAACG CCCGTCGTGG CCAGCCACGA TAGCCGCGCT 5521 GCCTCGTCTT GCAGTTCATT CAGGGCACCG GACAGGTCGG TCTTGACAAA AAGAACCGGG 5581 CGCCCCTGCG CTGACAGCCG GAACACGGCG GCATCAGAGC AGCCGATTGT CTGTTGTGCC 5641 CAGTCATAGC CGAATAGCCT CTCCACCCAA GCGGCCGGAG AACCTGCGTG CAATCCATCT 5701 TGTTCAATCA TGCGAAACGA TCCTCATCCT GTCTCTTGAT CGATCTTTGC AAAAGCCTAG 5761 GCCTCCAAAA AAGCCTCCTC ACTACTTCTG GAATAGCTCA GAGGCCGAGG CGGCCTCGGC 5821 CTCTGCATAA ATAAAAAAAA TTAGTCAGCC ATGGGGCGGA GAATGGGCGG AACTGGGCGG 5881 AGTTAGGGGC GGGATGGGCG GAGTTAGGGG CGGGACTATG GTTGCTGACT AATTGAGATG 5941 CATGCTTTGC ATACTTCTGC CTGCTGGGGA GCCTGGGGAC TTTCCACACC TGGTTGCTGA 6001 CTAATTGAGA TGCATGCTTT GCATACTTCT GCCTGCTGGG GAGCCTGGGG ACTTTCCACA 6061 CCCTAACTGA CACACATTCC ACAGCTGGTT CTTTCCGCCT CAGGACTCTT CCTTTTTCAA 6121 TATTATTGAA GCATTTATCA GGGTTATTGT CTCATGAGCG GATACATATT TGAATGTATT 6181 TAGAAAAATA AACAAATAGG GGTTCCGCGC ACATTTCCCC GAAAAGTGCC ACCTGACGCG 6241 CCCTGTAGCG GCGCATTAAG CGCGGCGGGT GTGGTGGTTA CGCGCAGCGT GACCGCTACA 6301 CTTGCCAGCG CCCTAGCGCC CGCTCCTTTC GCTTTCTTCC CTTCCTTTCT CGCCACGTTC 6361 GCCGGCTTTC CCCGTCAAGC TCTAAATCGG GGGCTCCCTT TAGGGTTCCG ATTTAGTGCT 6421 TTACGGCACC TCGACCCCAA AAAACTTGAT TAGGGTGATG GTTCACGTAG TGGGCCATCG 6481 CCCTGATAGA CGGTTTTTCG CCCTTTGACG TTGGAGTCCA CGTTCTTTAA TAGTGGACTC 6541 TTGTTCCAAA CTGGAACAAC ACTCAACCCT ATCTCGGTCT ATTCTTTTGA TTTATAAGGG 6601 ATTTTGCCGA TTTCGGCCTA TTGGTTAAAA AATGAGCTGA TTTAACAAAA ATTTAACGCG 6661 AATTTT //