LOCUS       RC213269                8634 bp    DNA     circular  SYN 
DEFINITION  AFF3 (Myc-DDK-tagged)-Human AF4/FMR2 family, member 3 (AFF3), transcript variant 2
ACCESSION   NM_001025108
VERSION     NM_001025108.1
SOURCE      synthetic construct 
FEATURES    Location/Qualifiers 
     misc_feature    201..924 
                     /label="CMV promoter"  
     misc_feature    839..856 
                     /label="Restriction Enzyme"  
     misc_feature    953..971 
                     /label="T7 promoter"  
     misc_feature    4791..4882 
                     /label="Myc-DDK"  
     misc_feature    4912..5497 
                     /label="PolyA signal"  
     misc_feature    4969..4988 
                     /label="Restriction Enzyme"  
     misc_feature    5773..6445 
                     /label="ColE1"  
     misc_feature    6875..7669 
                     /label="Kan/Neo"  
     misc_feature    7704..8006 
                     /label="SV40 ori"  
     misc_feature    8092..8569 
                     /label="f1 ori"  
     misc_feature    1020..1028 
                     /label="Restriction Enzyme"  
     misc_feature    4773..4779 
                     /label="Restriction Enzyme"  
     misc_feature    1020..4773 
                     /label="AFF3"  
ORIGIN                      
        1 AACAAAATAT TAACGCTTAC AATTTCCATT CGCCATTCAG GCTGCGCAAC TGTTGGGAAG 
       61 GGCGATCGGT GCGGGCCTCT TCGCTATTAC GCCAGCTGGC GAAAGGGGGA TGTGCTGCAA 
      121 GGCGATTAAG TTGGGTAACG CCAGGGTTTT CCCAGTCACG ACGTTGTAAA ACGACGGCCA 
      181 GTGCCAAGCT GATCTATACA TTGAATCAAT ATTGGCAATT AGCCATATTA GTCATTGGTT 
      241 ATATAGCATA AATCAATATT GGCTATTGGC CATTGCATAC GTTGTATCTA TATCATAATA 
      301 TGTACATTTA TATTGGCTCA TGTCCAATAT GACCGCCATG TTGACATTGA TTATTGACTA 
      361 GTTATTAATA GTAATCAATT ACGGGGTCAT TAGTTCATAG CCCATATATG GAGTTCCGCG 
      421 TTACATAACT TACGGTAAAT GGCCCGCCTG GCTGACCGCC CAACGACCCC CGCCCATTGA 
      481 CGTCAATAAT GACGTATGTT CCCATAGTAA CGCCAATAGG GACTTTCCAT TGACGTCAAT 
      541 GGGTGGAGTA TTTACGGTAA ACTGCCCACT TGGCAGTACA TCAAGTGTAT CATATGCCAA 
      601 GTCCGCCCCC TATTGACGTC AATGACGGTA AATGGCCCGC CTGGCATTAT GCCCAGTACA 
      661 TGACCTTACG GGACTTTCCT ACTTGGCAGT ACATCTACGT ATTAGTCATC GCTATTACCA 
      721 TGGTGATGCG GTTTTGGCAG TACACCAATG GGCGTGGATA GCGGTTTGAC TCACGGGGAT 
      781 TTCCAAGTCT CCACCCCATT GACGTCAATG GGAGTTTGTT TTGGCACCAA AATCAACGGG 
      841 ACTTTCCAAA ATGTCGTAAT AACCCCGCCC CGTTGACGCA AATGGGCGGT AGGCGTGTAC 
      901 GGTGGGAGGT CTATATAAGC AGAGCTCGTT TAGTGAACCG TCAGAATTTT GTAATACGAC 
      961 TCACTATAGG GCGGCCGGGA ATTCGTCGAC TGGATCCGGT ACCGAGGAGA TCTGCCGCCG 
     1021 CGATCGCCAT GGACAGCTTC GACTTAGCCC TGCTCCAGGA ATGGGACCTC GAGTCACTGT 
     1081 GGGGTGAAGA CATCTTAAAC CAGAGGAATG ACTCTCTAGT TGTGGAATTT CAGTCATCAG 
     1141 CCAGCAGATG CAGGAGTGTC TATGAACCAG ATAGAAATGC ATTACGGAGG AAAGAACGAG 
     1201 AAAGAAGAAA TCAAGAAACT CAACAGGATG ATGGCACGTT TAATTCTAGT TACTCTCTCT 
     1261 TCAGTGAGCC CTACAAGACT AACAAGGGGG ATGAACTCTC CAACCGGATC CAGAACACTT 
     1321 TAGGCAATTA TGATGAAATG AAAGACTTTT TAACTGATAG ATCCAATCAG AGTCATCTCG 
     1381 TTGGAGTTCC CAAACCTGGG GTTCCTCAGA CTCCTGTGAA CAAGATCGAT GAACATTTTG 
     1441 TTGCAGATTC AAGAGCCCAG AACCAGCCCT CGTCTATCTG TAGCACTACA ACTTCCACAC 
     1501 CAGCAGCTGT CCCCGTGCAG CAGAGTAAGA GAGGCACTAT GGGCTGGCAG AAGGCTGGGC 
     1561 ACCCACCCTC TGACGGCCAA CAGAGAGCAA CACAACAGGG CTCTCTCAGG ACCTTGCTTG 
     1621 GAGATGGTGT TGGCAGACAG CAGCCTCGGG CCAAACAAGT GTGCAATGTG GAGGTGGGCC 
     1681 TTCAGACCCA GGAGAGGCCA CCTGCCATGG CGGCCAAGCA CAGCAGCAGC GGACACTGTG 
     1741 TTCAGAACTT TCCTCCATCC CTAGCTTCAA AACCCAGCCT GGTCCAGCAG AAACCGACCG 
     1801 CGTATGTGAG GCCAATGGAC GGCCAAGATC AGGCCCCTGA TGAGTCTCCT AAGCTGAAGT 
     1861 CGTCTTCGGA AACCAGCGTG CACTGCACAT CATACAGGGG AGTCCCTGCC AGCAAGCCGG 
     1921 AGCCTGCCAG AGCCAAGGCC AAGCTCTCCA AGTTCAGCAT CCCCAAGCAG GGGGAGGAGA 
     1981 GTAGATCTGG AGAAACCAAC AGCTGTGTTG AAGAAATAAT CCGGGAGATG ACCTGGCTTC 
     2041 CACCACTTTC TGCTATTCAA GCACCTGGCA AAGTGGAACC AACCAAATTT CCATTTCCAA 
     2101 ATAAGGACTC TCAGCTTGTA TCCTCTGGAC ACAATAATCC AAAGAAAGGT GATGCAGAGC 
     2161 CAGAGAGTCC AGACAATGGC ACATCGAATA CATCAATGCT GGAAGATGAC CTTAAGCTAA 
     2221 GCAGTGATGA AGAGGAGAAT GAACAGCAGG CAGCTCAGAG AACGGCTCTC CGCGCTCTCT 
     2281 CTGACAGCGC CGTGGTCCAG CAGCCCAACT GCAGAACCTC GGTGCCTTCC AGCAAGGGCA 
     2341 GCAGCAGCAG CAGCAGCAGC GGCAGCAGCA GCTCCTCCAG CGACTCAGAG AGCAGCTCCG 
     2401 GATCTGACTC GGAGACCGAG AGCAGCTCCA GCGAGAGTGA GGGCAGCAAG CCCCCCCACT 
     2461 TCTCCAGCCC CGAGGCTGAA CCGGCATCCT CTAACAAGTG GCAGCTGGAT AAATGGCTAA 
     2521 ACAAAGTTAA TCCCCACAAG CCTCCTATTC TGATCCAAAA TGAAAGCCAC GGGTCAGAGA 
     2581 GCAATCAGTA CTACAACCCG GTGAAAGAGG ACGTCCAGGA CTGTGGGAAA GTCCCCGACG 
     2641 TTTGCCAGCC CAGCCTGAGA GAGAAGGAGA TCAAGAGCAC TTGCAAGGAG GAGCAAAGGC 
     2701 CAAGGACAGC CAACAAGGCC CCTGGGAGTA AAGGCGTGAA GCAGAAGTCC CCGCCCGCGG 
     2761 CCGTGGCCGT GGCGGTGAGC GCAGCCGCCC CGCCACCCGC AGTGCCCTGT GCGCCCGCGG 
     2821 AGAACGCGCC CGCGCCTGCC CGGAGGTCCG CGGGCAAGAA GCCCACCAGG CGCACCGAGA 
     2881 GGACCTCAGC CGGGGACGGC GCCAACTGCC ACCGGCCCGA GGAGCCCGCG GCCGCGGACG 
     2941 CGCTGGGGAC GAGCGTGGTG GTCCCCCCGG AGCCCACCAA AACCAGGCCC TGTGGCAACA 
     3001 ACAGAGCGAG CCACCGCAAG GAGCTGCGCT CCTCCGTGAC CTGCGAGAAG CGCCGCACGC 
     3061 GGGGGCTAAG CAGGATCGTC CCCAAATCCA AGGAGTTCAT TGAGACAGAG TCGTCATCTT 
     3121 CATCCTCCTC CTCGGACTCC GACCTGGAGT CCGAGCAGGA GGAGTACCCT CTGTCCAAAG 
     3181 CACAGACCGT GGCTGCCTCT GCCTCCTCCG GGAATGATCA GAGGCTGAAG GAGGCCGCTG 
     3241 CCAACGGGGG CAGTGGTCCT AGGGCCCCTG TAGGCTCCAT CAACGCCAGG ACCACCAGTG 
     3301 ACATCGCCAA GGAGCTGGAG GAGCAGTTCT ACACACTGGT CCCCTTTGGC CGGAACGAAC 
     3361 TTCTCTCCCC TCTAAAGGAC AGTGATGAGA TCAGGTCTCT CTGGGTCAAA ATCGACCTGA 
     3421 CCCTCCTGTC CAGGATCCCA GAACACCTGC CCCAGGAGCC AGGGGTATTG AGCGCCCCTG 
     3481 CCACCAAGGA CTCTGAGAGC GCACCGCCCA GCCACACCTC GGACACACCT GCAGAAAAGG 
     3541 CTTTGCCAAA ATCCAAGAGG AAACGCAAGT GTGACAACGA AGACGACTAC AGGGAGATCA 
     3601 AGAAGTCCCA GGGAGAGAAA GACAGCTCTT CAAGACTGGC CACCTCCACC AGTAATACTT 
     3661 TGTCTGCAAA CCACTGCAAC ATGAACATCA ACAGTGTGGC AATACCAATA AATAAAAATG 
     3721 AAAAAATGCT TCGGTCGCCC ATCTCACCCC TCTCTGATGC ATCTAAACAC AAATACACCA 
     3781 GCGAGGACTT AACTTCTTCC AGCCGACCTA ATGGCAACAG TTTGTTTACT TCAGCCTCTT 
     3841 CCAGCAAAAA GCCTAAGGCC GACAGCCAGC TGCAGCCTCA CGGCGGAGAC CTCACGAAAG 
     3901 CAGCTCACAA CAATTCTGAA AACATTCCCC TCCACAAGTC ACGGCCGCAG ACGAAGCCGT 
     3961 GGTCTCCAGG CTCCAACGGC CACAGGGACT GCAAGAGGCA GAAACTTGTC TTCGATGATA 
     4021 TGCCTCGCAG TGCCGATTAT TTTATGCAAG AAGCTAAACG AATGAAGCAT AAAGCAGATG 
     4081 CAATGGTGGA AAAGTTTGGA AAGGCTTTGA ACTATGCTGA AGCAGCATTG TCGTTTATCG 
     4141 AGTGTGGAAA TGCAATGGAA CAAGGCCCCA TGGAATCCAA ATCTCCTTAT ACGATGTATT 
     4201 CAGAAACAGT AGAGCTCATC AGGTATGCTA TGAGACTAAA AACCCACTCA GGCCCCAATG 
     4261 CCACACCAGA AGACAAACAA CTGGCTGCAT TATGTTACCG ATGCCTGGCC CTCCTGTACT 
     4321 GGCGGATGTT TCGACTCAAA AGGGACCACG CTGTAAAGTA TTCAAAAGCA CTAATCGACT 
     4381 ATTTCAAGAA CTCATCTAAA GCCGCCCAAG CCCCATCTCC GTGGGGGGCC AGTGGAAAGA 
     4441 GCACTGGAAC CCCATCCCCC ATGTCTCCCA ACCCCTCTCC CGCCAGCTCC GTGGGGTCTC 
     4501 AGGGCAGCCT CTCCAACGCC AGCGCCCTGT CCCCGTCGAC CATCGTCAGC ATCCCACAGC 
     4561 GCATCCACCA GATGGCGGCC AACCACGTCA GCATCACCAA CAGCATCCTG CACAGCTACG 
     4621 ACTACTGGGA GATGGCCGAC AACCTGGCCA AGGAAAACCG AGAATTCTTC AACGACCTGG 
     4681 ATCTGCTCAT GGGGCCGGTC ACCCTGCACA GCAGCATGGA GCACCTGGTC CAGTACTCCC 
     4741 AACAGGGCCT GCACTGGCTG CGGAACAGCG CCCACCTGTC AACGCGTACG CGGCCGCTCG 
     4801 AGCAGAAACT CATCTCAGAA GAGGATCTGG CAGCAAATGA TATCCTGGAT TACAAGGATG 
     4861 ACGACGATAA GGTTTAAACG GCCGGCCGCG GTCATAGCTG TTTCCTGAAC AGATCCCGGG 
     4921 TGGCATCCCT GTGACCCCTC CCCAGTGCCT CTCCTGGCCC TGGAAGTTGC CACTCCAGTG 
     4981 CCCACCAGCC TTGTCCTAAT AAAATTAAGT TGCATCATTT TGTCTGACTA GGTGTCCTTC 
     5041 TATAATATTA TGGGGTGGAG GGGGGTGGTA TGGAGCAAGG GGCAAGTTGG GAAGACAACC 
     5101 TGTAGGGCCT GCGGGGTCTA TTGGGAACCA AGCTGGAGTG CAGTGGCACA ATCTTGGCTC 
     5161 ACTGCAATCT CCGCCTCCTG GGTTCAAGCG ATTCTCCTGC CTCAGCCTCC CGAGTTGTTG 
     5221 GGATTCCAGG CATGCATGAC CAGGCTCAGC TAATTTTTGT TTTTTTGGTA GAGACGGGGT 
     5281 TTCACCATAT TGGCCAGGCT GGTCTCCAAC TCCTAATCTC AGGTGATCTA CCCACCTTGG 
     5341 CCTCCCAAAT TGCTGGGATT ACAGGCGTGA ACCACTGCTC CCTTCCCTGT CCTTCTGATT 
     5401 TTAAAATAAC TATACCAGCA GGAGGACGTC CAGACACAGC ATAGGCTACC TGGCCATGCC 
     5461 CAACCGGTGG GACATTTGAG TTGCTTGCTT GGCACTGTCC TCTCATGCGT TGGGTCCACT 
     5521 CAGTAGATGC CTGTTGAATT GGGTACGCGG CCAGCGGCGA GCGGTATCAG CTCACTCAAA 
     5581 GGCGGTAATA CGGTTATCCA CAGAATCAGG GGATAACGCA GGAAAGAACA TGTGAGCAAA 
     5641 AGGCCAGCAA AAGGCCAGGA ACCGTAAAAA GGCCGCGTTG CTGGCGTTTT TCCATAGGCT 
     5701 CCGCCCCCCT GACGAGCATC ACAAAAATCG ACGCTCAAGT CAGAGGTGGC GAAACCCGAC 
     5761 AGGACTATAA AGATACCAGG CGTTTCCCCC TGGAAGCTCC CTCGTGCGCT CTCCTGTTCC 
     5821 GACCCTGCCG CTTACCGGAT ACCTGTCCGC CTTTCTCCCT TCGGGAAGCG TGGCGCTTTC 
     5881 TCATAGCTCA CGCTGTAGGT ATCTCAGTTC GGTGTAGGTC GTTCGCTCCA AGCTGGGCTG 
     5941 TGTGCACGAA CCCCCCGTTC AGCCCGACCG CTGCGCCTTA TCCGGTAACT ATCGTCTTGA 
     6001 GTCCAACCCG GTAAGACACG ACTTATCGCC ACTGGCAGCA GCCACTGGTA ACAGGATTAG 
     6061 CAGAGCGAGG TATGTAGGCG GTGCTACAGA GTTCTTGAAG TGGTGGCCTA ACTACGGCTA 
     6121 CACTAGAAGA ACAGTATTTG GTATCTGCGC TCTGCTGAAG CCAGTTACCT TCGGAAAAAG 
     6181 AGTTGGTAGC TCTTGATCCG GCAAACAAAC CACCGCTGGT AGCGGTGGTT TTTTTGTTTG 
     6241 CAAGCAGCAG ATTACGCGCA GAAAAAAAGG ATCTCAAGAA GATCCTTTGA TCTTTTCTAC 
     6301 GGGGTCTGAC GCTCAGTGGA ACGAAAACTC ACGTTAAGGG ATTTTGGTCA TGAGATTATC 
     6361 AAAAAGGATC TTCACCTAGA TCCTTTTAAA TTAAAAATGA AGTTTTAAAT CAATCTAAAG 
     6421 TATATATGAG TAACCTGAGG CTATGGCAGG GCCTGCCGCC CCGACGTTGG CTGCGAGCCC 
     6481 TGGGCCTTCA CCCGAACTTG GGGGGTGGGG TGGGGAAAAG GAAGAAACGC GGGCGTATTG 
     6541 GCCCCAATGG GGTCTCGGTG GGGTATCGAC AGAGTGCCAG CCCTGGGACC GAACCCCGCG 
     6601 TTTATGAACA AACGACCCAA CACCGTGCGT TTTATTCTGT CTTTTTATTG CCGTCATAGC 
     6661 GCGGGTTCCT TCCGGTATTG TCTCCTTCCG TGTTTCAGTT AGCCTCCCCC TAGGGTGGGC 
     6721 GAAGAACTCC AGCATGAGAT CCCCGCGCTG GAGGATCATC CAGCCGGCGT CCCGGAAAAC 
     6781 GATTCCGAAG CCCAACCTTT CATAGAAGGC GGCGGTGGAA TCGAAATCTC GTGATGGCAG 
     6841 GTTGGGCGTC GCTTGGTCGG TCATTTCGAA CCCCAGAGTC CCGCTCAGAA GAACTCGTCA 
     6901 AGAAGGCGAT AGAAGGCGAT GCGCTGCGAA TCGGGAGCGG CGATACCGTA AAGCACGAGG 
     6961 AAGCGGTCAG CCCATTCGCC GCCAAGCTCT TCAGCAATAT CACGGGTAGC CAACGCTATG 
     7021 TCCTGATAGC GATCCGCCAC ACCCAGCCGG CCACAGTCGA TGAATCCAGA AAAGCGGCCA 
     7081 TTTTCCACCA TGATATTCGG CAAGCAGGCA TCGCCATGGG TCACGACGAG ATCCTCGCCG 
     7141 TCGGGCATGC TCGCCTTGAG CCTGGCGAAC AGTTCGGCTG GCGCGAGCCC CTGATGCTCT 
     7201 TCGTCCAGAT CATCCTGATC GACAAGACCG GCTTCCATCC GAGTACGTGC TCGCTCGATG 
     7261 CGATGTTTCG CTTGGTGGTC GAATGGGCAG GTAGCCGGAT CAAGCGTATG CAGCCGCCGC 
     7321 ATTGCATCAG CCATGATGGA TACTTTCTCG GCAGGAGCAA GGTGAGATGA CAGGAGATCC 
     7381 TGCCCCGGCA CTTCGCCCAA TAGCAGCCAG TCCCTTCCCG CTTCAGTGAC AACGTCGAGC 
     7441 ACAGCTGCGC AAGGAACGCC CGTCGTGGCC AGCCACGATA GCCGCGCTGC CTCGTCTTGC 
     7501 AGTTCATTCA GGGCACCGGA CAGGTCGGTC TTGACAAAAA GAACCGGGCG CCCCTGCGCT 
     7561 GACAGCCGGA ACACGGCGGC ATCAGAGCAG CCGATTGTCT GTTGTGCCCA GTCATAGCCG 
     7621 AATAGCCTCT CCACCCAAGC GGCCGGAGAA CCTGCGTGCA ATCCATCTTG TTCAATCATG 
     7681 CGAAACGATC CTCATCCTGT CTCTTGATCG ATCTTTGCAA AAGCCTAGGC CTCCAAAAAA 
     7741 GCCTCCTCAC TACTTCTGGA ATAGCTCAGA GGCCGAGGCG GCCTCGGCCT CTGCATAAAT 
     7801 AAAAAAAATT AGTCAGCCAT GGGGCGGAGA ATGGGCGGAA CTGGGCGGAG TTAGGGGCGG 
     7861 GATGGGCGGA GTTAGGGGCG GGACTATGGT TGCTGACTAA TTGAGATGCA TGCTTTGCAT 
     7921 ACTTCTGCCT GCTGGGGAGC CTGGGGACTT TCCACACCTG GTTGCTGACT AATTGAGATG 
     7981 CATGCTTTGC ATACTTCTGC CTGCTGGGGA GCCTGGGGAC TTTCCACACC CTAACTGACA 
     8041 CACATTCCAC AGCTGGTTCT TTCCGCCTCA GGACTCTTCC TTTTTCAATA TTATTGAAGC 
     8101 ATTTATCAGG GTTATTGTCT CATGAGCGGA TACATATTTG AATGTATTTA GAAAAATAAA 
     8161 CAAATAGGGG TTCCGCGCAC ATTTCCCCGA AAAGTGCCAC CTGACGCGCC CTGTAGCGGC 
     8221 GCATTAAGCG CGGCGGGTGT GGTGGTTACG CGCAGCGTGA CCGCTACACT TGCCAGCGCC 
     8281 CTAGCGCCCG CTCCTTTCGC TTTCTTCCCT TCCTTTCTCG CCACGTTCGC CGGCTTTCCC 
     8341 CGTCAAGCTC TAAATCGGGG GCTCCCTTTA GGGTTCCGAT TTAGTGCTTT ACGGCACCTC 
     8401 GACCCCAAAA AACTTGATTA GGGTGATGGT TCACGTAGTG GGCCATCGCC CTGATAGACG 
     8461 GTTTTTCGCC CTTTGACGTT GGAGTCCACG TTCTTTAATA GTGGACTCTT GTTCCAAACT 
     8521 GGAACAACAC TCAACCCTAT CTCGGTCTAT TCTTTTGATT TATAAGGGAT TTTGCCGATT 
     8581 TCGGCCTATT GGTTAAAAAA TGAGCTGATT TAACAAAAAT TTAACGCGAA TTTT
//