LOCUS       RC213240                8700 bp    DNA     circular  SYN 
DEFINITION  SIN3A (Myc-DDK-tagged)-Human SIN3 homolog A, transcription regulator (yeast) (SIN3A), transcript variant 2
ACCESSION   NM_015477
VERSION     NM_015477.1
SOURCE      synthetic construct 
FEATURES    Location/Qualifiers 
     misc_feature    201..924 
                     /label="CMV promoter"  
     misc_feature    839..856 
                     /label="Restriction Enzyme"  
     misc_feature    953..971 
                     /label="T7 promoter"  
     misc_feature    4857..4948 
                     /label="Myc-DDK"  
     misc_feature    4978..5563 
                     /label="PolyA signal"  
     misc_feature    5035..5054 
                     /label="Restriction Enzyme"  
     misc_feature    5839..6511 
                     /label="ColE1"  
     misc_feature    6941..7735 
                     /label="Kan/Neo"  
     misc_feature    7770..8072 
                     /label="SV40 ori"  
     misc_feature    8158..8635 
                     /label="f1 ori"  
     misc_feature    1020..1028 
                     /label="Restriction Enzyme"  
     misc_feature    4839..4845 
                     /label="Restriction Enzyme"  
     misc_feature    1020..4839 
                     /label="SIN3A"  
ORIGIN                      
        1 AACAAAATAT TAACGCTTAC AATTTCCATT CGCCATTCAG GCTGCGCAAC TGTTGGGAAG 
       61 GGCGATCGGT GCGGGCCTCT TCGCTATTAC GCCAGCTGGC GAAAGGGGGA TGTGCTGCAA 
      121 GGCGATTAAG TTGGGTAACG CCAGGGTTTT CCCAGTCACG ACGTTGTAAA ACGACGGCCA 
      181 GTGCCAAGCT GATCTATACA TTGAATCAAT ATTGGCAATT AGCCATATTA GTCATTGGTT 
      241 ATATAGCATA AATCAATATT GGCTATTGGC CATTGCATAC GTTGTATCTA TATCATAATA 
      301 TGTACATTTA TATTGGCTCA TGTCCAATAT GACCGCCATG TTGACATTGA TTATTGACTA 
      361 GTTATTAATA GTAATCAATT ACGGGGTCAT TAGTTCATAG CCCATATATG GAGTTCCGCG 
      421 TTACATAACT TACGGTAAAT GGCCCGCCTG GCTGACCGCC CAACGACCCC CGCCCATTGA 
      481 CGTCAATAAT GACGTATGTT CCCATAGTAA CGCCAATAGG GACTTTCCAT TGACGTCAAT 
      541 GGGTGGAGTA TTTACGGTAA ACTGCCCACT TGGCAGTACA TCAAGTGTAT CATATGCCAA 
      601 GTCCGCCCCC TATTGACGTC AATGACGGTA AATGGCCCGC CTGGCATTAT GCCCAGTACA 
      661 TGACCTTACG GGACTTTCCT ACTTGGCAGT ACATCTACGT ATTAGTCATC GCTATTACCA 
      721 TGGTGATGCG GTTTTGGCAG TACACCAATG GGCGTGGATA GCGGTTTGAC TCACGGGGAT 
      781 TTCCAAGTCT CCACCCCATT GACGTCAATG GGAGTTTGTT TTGGCACCAA AATCAACGGG 
      841 ACTTTCCAAA ATGTCGTAAT AACCCCGCCC CGTTGACGCA AATGGGCGGT AGGCGTGTAC 
      901 GGTGGGAGGT CTATATAAGC AGAGCTCGTT TAGTGAACCG TCAGAATTTT GTAATACGAC 
      961 TCACTATAGG GCGGCCGGGA ATTCGTCGAC TGGATCCGGT ACCGAGGAGA TCTGCCGCCG 
     1021 CGATCGCCAT GAAGCGGCGT TTGGATGACC AGGAGTCACC GGTGTATGCA GCCCAGCAGC 
     1081 GTCGGATCCC TGGCAGCACA GAGGCTTTTC CTCACCAGCA CCGGGTGCTT GCCCCTGCCC 
     1141 CTCCTGTGTA TGAAGCAGTG TCTGAGACCA TGCAGTCAGC TACGGGAATT CAGTACTCTG 
     1201 TAACACCCAG CTACCAGGTT TCAGCCATGC CACAGAGCTC CGGCAGTCAT GGGCCCGCTA 
     1261 TAGCAGCAGT TCATAGCAGC CATCATCACC CAACAGCGGT GCAGCCCCAC GGAGGCCAGG 
     1321 TGGTCCAGAG TCATGCTCAT CCAGCCCCAC CAGTTGCACC AGTGCAGGGA CAGCAGCAAT 
     1381 TTCAGAGGCT GAAGGTGGAG GATGCGCTAT CTTATCTTGA CCAGGTGAAG CTGCAGTTTG 
     1441 GTAGTCAGCC TCAGGTCTAC AATGATTTCC TTGACATCAT GAAGGAATTT AAATCTCAGA 
     1501 GCATCGACAC CCCAGGAGTG ATTAGTCGTG TGTCCCAGCT ATTCAAAGGC CACCCCGATC 
     1561 TGATAATGGG ATTCAACACC TTCTTGCCCC CTGGCTACAA AATTGAGGTG CAAACCAATG 
     1621 ACATGGTGAA TGTGACAACT CCTGGCCAGG TTCATCAGAT TCCCACCCAT GGCATCCAGC 
     1681 CACAGCCTCA ACCACCACCC CAACATCCTT CCCAGCCTTC AGCCCAGTCA GCCCCAGCTC 
     1741 CTGCCCAGCC AGCTCCTCAG CCCCCACCTG CCAAAGTCAG CAAGCCCTCC CAACTGCAAG 
     1801 CACATACTCC GGCCAGTCAG CAGACTCCCC CACTTCCACC GTATGCATCC CCACGTTCTC 
     1861 CGCCGGTCCA GCCTCACACA CCAGTGACAA TCTCGTTGGG AACGGCCCCA TCCTTGCAGA 
     1921 ACAATCAACC TGTGGAGTTT AATCATGCCA TCAACTATGT TAATAAGATC AAGAACAGAT 
     1981 TTCAGGGCCA ACCAGACATC TACAAAGCAT TCCTGGAGAT TTTGCACACA TATCAGAAAG 
     2041 AGCAGAGAAA TGCCAAGGAA GCTGGAGGAA ACTACACTCC AGCATTGACA GAGCAGGAGG 
     2101 TGTATGCCCA GGTTGCTCGT CTCTTTAAAA ACCAGGAAGA TTTGTTGTCA GAGTTTGGAC 
     2161 AATTCCTACC AGATGCCAAC AGCTCCGTGC TTTTAAGCAA AACAACTGCT GAGAAGGTTG 
     2221 ATTCTGTGAG AAATGATCAT GGAGGCACTG TCAAGAAGCC CCAACTGAAC AACAAGCCGC 
     2281 AGAGGCCCAG CCAGAATGGC TGCCAGATCC GCAGACATCC TACAGGAACC ACCCCTCCAG 
     2341 TTAAGAAGAA ACCCAAACTG CTCAATCTGA AGGATTCTTC TATGGCAGAT GCCAGCAAAC 
     2401 ATAGTGGTGG AACAGAATCG TTATTTTTTG ATAAGGTCCG AAAGGCTCTT CGGAGTGCAG 
     2461 AAGCCTACGA AAATTTCCTA CGCTGTCTTG TTATTTTTAA CCAGGAGGTG ATCTCTCGTG 
     2521 CTGAGCTTGT GCAACTAGTC TCTCCTTTCC TGGGGAAATT CCCTGAGTTG TTTAATTGGT 
     2581 TTAAAAACTT TCTGGGCTAT AAGGAGTCTG TACATCTGGA AACTTATCCA AAGGAGCGAG 
     2641 CCACAGAGGG CATTGCTATG GAGATAGATT ATGCTTCTTG TAAACGATTG GGCTCCAGCT 
     2701 ATCGAGCCTT ACCAAAGAGT TACCAGCAGC CCAAGTGTAC AGGACGGACT CCTCTCTGTA 
     2761 AAGAGGTTTT AAATGATACC TGGGTTTCCT TCCCTTCGTG GTCTGAGGAC TCTACCTTTG 
     2821 TGAGTTCCAA GAAGACTCAA TATGAAGAAC ATATTTATCG TTGTGAAGAT GAACGCTTTG 
     2881 AGCTTGATGT AGTTTTAGAG ACCAATCTGG CAACAATCCG GGTTCTGGAA GCAATACAGA 
     2941 AGAAGCTTTC CCGCTTGTCT GCTGAAGAAC AAGCCAAATT TCGCTTGGAC AACACCCTTG 
     3001 GGGGCACATC AGAAGTCATC CATAGAAAAG CACTCCAGAG GATATATGCT GATAAAGCAG 
     3061 CTGACATCAT TGATGGTCTG AGAAAGAATC CCTCCATTGC TGTTCCAATT GTCCTTAAAA 
     3121 GGTTGAAGAT GAAAGAGGAA GAATGGCGAG AAGCTCAGAG AGGCTTTAAC AAAGTATGGC 
     3181 GAGAACAAAA TGAGAAATAC TACTTGAAGT CTCTGGACCA CCAGGGGATC AACTTTAAAC 
     3241 AGAATGACAC CAAGGTCCTG AGGTCTAAGA GCTTACTCAA TGAGATTGAG AGTATCTATG 
     3301 ATGAGAGGCA AGAGCAGGCT ACGGAGGAGA ATGCTGGTGT ACCTGTTGGC CCACACCTCT 
     3361 CACTTGCGTA TGAAGACAAA CAAATACTGG AAGATGCTGC TGCTCTGATT ATCCACCATG 
     3421 TGAAGAGGCA GACAGGCATT CAGAAGGAGG ACAAATATAA GATAAAACAA ATCATGCATC 
     3481 ATTTTATTCC AGATTTGCTC TTTGCCCAAA GAGGTGATCT CTCAGATGTG GAGGAAGAGG 
     3541 AAGAAGAAGA GATGGATGTA GATGAAGCCA CAGGGGCAGT TAAGAAGCAC AATGGTGTTG 
     3601 GGGGCAGTCC CCCTAAGTCC AAGTTACTGT TTAGTAACAC AGCAGCTCAA AAATTAAGAG 
     3661 GAATGGATGA AGTATACAAC CTCTTCTATG TCAACAACAA CTGGTATATT TTTATGCGAC 
     3721 TGCACCAGAT TCTCTGCCTG AGGCTGCTAC GGATTTGTTC CCAAGCCGAA CGGCAAATTG 
     3781 AAGAAGAAAA CCGAGAGAGA GAATGGGAAC GGGAAGTGCT GGGCATAAAG CGAGACAAGA 
     3841 GTGACAGCCC TGCCATTCAG CTACGTCTCA AAGAACCTAT GGATGTTGAT GTAGAAGATT 
     3901 ATTACCCAGC TTTCCTGGAC ATGGTGCGGA GCCTGCTGGA TGGCAACATA GACTCATCAC 
     3961 AGTATGAAGA TTCACTGAGA GAGATGTTCA CCATTCATGC CTACATTGCC TTTACCATGG 
     4021 ACAAACTGAT CCAGAGCATT GTCAGACAGC TGCAGCATAT CGTGAGTGAT GAGATCTGTG 
     4081 TGCAGGTGAC TGACCTTTAC CTGGCAGAAA ATAATAATGG GGCCACCGGA GGCCAGCTGA 
     4141 ACACACAGAA CTCAAGGAGC CTCCTGGAGT CAACGTATCA GCGGAAAGCT GAGCAGCTAA 
     4201 TGTCAGATGA GAATTGCTTT AAGCTTATGT TTATTCAGAG CCAAGGCCAG GTCCAGCTGA 
     4261 CTATTGAGCT TCTGGACACA GAAGAGGAGA ATTCGGATGA CCCTGTGGAA GCAGAGCGCT 
     4321 GGTCAGACTA CGTGGAGCGA TACATGAATT CAGATACTAC CTCGCCTGAG CTTCGTGAAC 
     4381 ATCTAGCACA GAAACCAGTA TTTCTCCCCA GGAATCTACG GCGGATCCGG AAGTGTCAAC 
     4441 GTGGTCGAGA GCAGCAGGAA AAGGAAGGGA AGGAAGGAAA CAGCAAGAAG ACCATGGAGA 
     4501 ATGTGGATAG TCTGGATAAG CTGGAGTGTA GATTCAAGCT GAATTCCTAC AAGATGGTGT 
     4561 ATGTGATCAA ATCAGAGGAC TATATGTATC GGAGGACCGC CCTGCTCCGG GCTCATCAGT 
     4621 CCCATGAGCG TGTAAGCAAG CGTCTACATC AGAGATTCCA GGCCTGGGTA GATAAATGGA 
     4681 CCAAGGAGCA TGTGCCCCGT GAAATGGCAG CAGAGACCAG CAAGTGGCTC ATGGGTGAGG 
     4741 GGCTGGAGGG CCTGGTGCCC TGTACCACCA CCTGTGATAC AGAGACCCTG CATTTTGTGA 
     4801 GCATTAACAA GTATCGTGTC AAATACGGCA CAGTATTCAA AGCCCCTACG CGTACGCGGC 
     4861 CGCTCGAGCA GAAACTCATC TCAGAAGAGG ATCTGGCAGC AAATGATATC CTGGATTACA 
     4921 AGGATGACGA CGATAAGGTT TAAACGGCCG GCCGCGGTCA TAGCTGTTTC CTGAACAGAT 
     4981 CCCGGGTGGC ATCCCTGTGA CCCCTCCCCA GTGCCTCTCC TGGCCCTGGA AGTTGCCACT 
     5041 CCAGTGCCCA CCAGCCTTGT CCTAATAAAA TTAAGTTGCA TCATTTTGTC TGACTAGGTG 
     5101 TCCTTCTATA ATATTATGGG GTGGAGGGGG GTGGTATGGA GCAAGGGGCA AGTTGGGAAG 
     5161 ACAACCTGTA GGGCCTGCGG GGTCTATTGG GAACCAAGCT GGAGTGCAGT GGCACAATCT 
     5221 TGGCTCACTG CAATCTCCGC CTCCTGGGTT CAAGCGATTC TCCTGCCTCA GCCTCCCGAG 
     5281 TTGTTGGGAT TCCAGGCATG CATGACCAGG CTCAGCTAAT TTTTGTTTTT TTGGTAGAGA 
     5341 CGGGGTTTCA CCATATTGGC CAGGCTGGTC TCCAACTCCT AATCTCAGGT GATCTACCCA 
     5401 CCTTGGCCTC CCAAATTGCT GGGATTACAG GCGTGAACCA CTGCTCCCTT CCCTGTCCTT 
     5461 CTGATTTTAA AATAACTATA CCAGCAGGAG GACGTCCAGA CACAGCATAG GCTACCTGGC 
     5521 CATGCCCAAC CGGTGGGACA TTTGAGTTGC TTGCTTGGCA CTGTCCTCTC ATGCGTTGGG 
     5581 TCCACTCAGT AGATGCCTGT TGAATTGGGT ACGCGGCCAG CGGCGAGCGG TATCAGCTCA 
     5641 CTCAAAGGCG GTAATACGGT TATCCACAGA ATCAGGGGAT AACGCAGGAA AGAACATGTG 
     5701 AGCAAAAGGC CAGCAAAAGG CCAGGAACCG TAAAAAGGCC GCGTTGCTGG CGTTTTTCCA 
     5761 TAGGCTCCGC CCCCCTGACG AGCATCACAA AAATCGACGC TCAAGTCAGA GGTGGCGAAA 
     5821 CCCGACAGGA CTATAAAGAT ACCAGGCGTT TCCCCCTGGA AGCTCCCTCG TGCGCTCTCC 
     5881 TGTTCCGACC CTGCCGCTTA CCGGATACCT GTCCGCCTTT CTCCCTTCGG GAAGCGTGGC 
     5941 GCTTTCTCAT AGCTCACGCT GTAGGTATCT CAGTTCGGTG TAGGTCGTTC GCTCCAAGCT 
     6001 GGGCTGTGTG CACGAACCCC CCGTTCAGCC CGACCGCTGC GCCTTATCCG GTAACTATCG 
     6061 TCTTGAGTCC AACCCGGTAA GACACGACTT ATCGCCACTG GCAGCAGCCA CTGGTAACAG 
     6121 GATTAGCAGA GCGAGGTATG TAGGCGGTGC TACAGAGTTC TTGAAGTGGT GGCCTAACTA 
     6181 CGGCTACACT AGAAGAACAG TATTTGGTAT CTGCGCTCTG CTGAAGCCAG TTACCTTCGG 
     6241 AAAAAGAGTT GGTAGCTCTT GATCCGGCAA ACAAACCACC GCTGGTAGCG GTGGTTTTTT 
     6301 TGTTTGCAAG CAGCAGATTA CGCGCAGAAA AAAAGGATCT CAAGAAGATC CTTTGATCTT 
     6361 TTCTACGGGG TCTGACGCTC AGTGGAACGA AAACTCACGT TAAGGGATTT TGGTCATGAG 
     6421 ATTATCAAAA AGGATCTTCA CCTAGATCCT TTTAAATTAA AAATGAAGTT TTAAATCAAT 
     6481 CTAAAGTATA TATGAGTAAC CTGAGGCTAT GGCAGGGCCT GCCGCCCCGA CGTTGGCTGC 
     6541 GAGCCCTGGG CCTTCACCCG AACTTGGGGG GTGGGGTGGG GAAAAGGAAG AAACGCGGGC 
     6601 GTATTGGCCC CAATGGGGTC TCGGTGGGGT ATCGACAGAG TGCCAGCCCT GGGACCGAAC 
     6661 CCCGCGTTTA TGAACAAACG ACCCAACACC GTGCGTTTTA TTCTGTCTTT TTATTGCCGT 
     6721 CATAGCGCGG GTTCCTTCCG GTATTGTCTC CTTCCGTGTT TCAGTTAGCC TCCCCCTAGG 
     6781 GTGGGCGAAG AACTCCAGCA TGAGATCCCC GCGCTGGAGG ATCATCCAGC CGGCGTCCCG 
     6841 GAAAACGATT CCGAAGCCCA ACCTTTCATA GAAGGCGGCG GTGGAATCGA AATCTCGTGA 
     6901 TGGCAGGTTG GGCGTCGCTT GGTCGGTCAT TTCGAACCCC AGAGTCCCGC TCAGAAGAAC 
     6961 TCGTCAAGAA GGCGATAGAA GGCGATGCGC TGCGAATCGG GAGCGGCGAT ACCGTAAAGC 
     7021 ACGAGGAAGC GGTCAGCCCA TTCGCCGCCA AGCTCTTCAG CAATATCACG GGTAGCCAAC 
     7081 GCTATGTCCT GATAGCGATC CGCCACACCC AGCCGGCCAC AGTCGATGAA TCCAGAAAAG 
     7141 CGGCCATTTT CCACCATGAT ATTCGGCAAG CAGGCATCGC CATGGGTCAC GACGAGATCC 
     7201 TCGCCGTCGG GCATGCTCGC CTTGAGCCTG GCGAACAGTT CGGCTGGCGC GAGCCCCTGA 
     7261 TGCTCTTCGT CCAGATCATC CTGATCGACA AGACCGGCTT CCATCCGAGT ACGTGCTCGC 
     7321 TCGATGCGAT GTTTCGCTTG GTGGTCGAAT GGGCAGGTAG CCGGATCAAG CGTATGCAGC 
     7381 CGCCGCATTG CATCAGCCAT GATGGATACT TTCTCGGCAG GAGCAAGGTG AGATGACAGG 
     7441 AGATCCTGCC CCGGCACTTC GCCCAATAGC AGCCAGTCCC TTCCCGCTTC AGTGACAACG 
     7501 TCGAGCACAG CTGCGCAAGG AACGCCCGTC GTGGCCAGCC ACGATAGCCG CGCTGCCTCG 
     7561 TCTTGCAGTT CATTCAGGGC ACCGGACAGG TCGGTCTTGA CAAAAAGAAC CGGGCGCCCC 
     7621 TGCGCTGACA GCCGGAACAC GGCGGCATCA GAGCAGCCGA TTGTCTGTTG TGCCCAGTCA 
     7681 TAGCCGAATA GCCTCTCCAC CCAAGCGGCC GGAGAACCTG CGTGCAATCC ATCTTGTTCA 
     7741 ATCATGCGAA ACGATCCTCA TCCTGTCTCT TGATCGATCT TTGCAAAAGC CTAGGCCTCC 
     7801 AAAAAAGCCT CCTCACTACT TCTGGAATAG CTCAGAGGCC GAGGCGGCCT CGGCCTCTGC 
     7861 ATAAATAAAA AAAATTAGTC AGCCATGGGG CGGAGAATGG GCGGAACTGG GCGGAGTTAG 
     7921 GGGCGGGATG GGCGGAGTTA GGGGCGGGAC TATGGTTGCT GACTAATTGA GATGCATGCT 
     7981 TTGCATACTT CTGCCTGCTG GGGAGCCTGG GGACTTTCCA CACCTGGTTG CTGACTAATT 
     8041 GAGATGCATG CTTTGCATAC TTCTGCCTGC TGGGGAGCCT GGGGACTTTC CACACCCTAA 
     8101 CTGACACACA TTCCACAGCT GGTTCTTTCC GCCTCAGGAC TCTTCCTTTT TCAATATTAT 
     8161 TGAAGCATTT ATCAGGGTTA TTGTCTCATG AGCGGATACA TATTTGAATG TATTTAGAAA 
     8221 AATAAACAAA TAGGGGTTCC GCGCACATTT CCCCGAAAAG TGCCACCTGA CGCGCCCTGT 
     8281 AGCGGCGCAT TAAGCGCGGC GGGTGTGGTG GTTACGCGCA GCGTGACCGC TACACTTGCC 
     8341 AGCGCCCTAG CGCCCGCTCC TTTCGCTTTC TTCCCTTCCT TTCTCGCCAC GTTCGCCGGC 
     8401 TTTCCCCGTC AAGCTCTAAA TCGGGGGCTC CCTTTAGGGT TCCGATTTAG TGCTTTACGG 
     8461 CACCTCGACC CCAAAAAACT TGATTAGGGT GATGGTTCAC GTAGTGGGCC ATCGCCCTGA 
     8521 TAGACGGTTT TTCGCCCTTT GACGTTGGAG TCCACGTTCT TTAATAGTGG ACTCTTGTTC 
     8581 CAAACTGGAA CAACACTCAA CCCTATCTCG GTCTATTCTT TTGATTTATA AGGGATTTTG 
     8641 CCGATTTCGG CCTATTGGTT AAAAAATGAG CTGATTTAAC AAAAATTTAA CGCGAATTTT 
//