LOCUS RC213190 7329 bp DNA circular SYN DEFINITION TPCN1 (Myc-DDK-tagged)-Human two pore segment channel 1 (TPCN1), transcript variant 2 ACCESSION NM_017901 VERSION null SOURCE synthetic construct FEATURES Location/Qualifiers misc_feature 201..924 /label="CMV promoter" misc_feature 839..856 /label="Restriction Enzyme" misc_feature 953..971 /label="T7 promoter" misc_feature 3486..3577 /label="Myc-DDK" misc_feature 3607..4192 /label="PolyA signal" misc_feature 3664..3683 /label="Restriction Enzyme" misc_feature 4468..5140 /label="ColE1" misc_feature 5570..6364 /label="Kan/Neo" misc_feature 6399..6701 /label="SV40 ori" misc_feature 6787..7264 /label="f1 ori" misc_feature 1020..1028 /label="Restriction Enzyme" misc_feature 3468..3474 /label="Restriction Enzyme" misc_feature 1020..3468 /label="TPCN1" ORIGIN 1 AACAAAATAT TAACGCTTAC AATTTCCATT CGCCATTCAG GCTGCGCAAC TGTTGGGAAG 61 GGCGATCGGT GCGGGCCTCT TCGCTATTAC GCCAGCTGGC GAAAGGGGGA TGTGCTGCAA 121 GGCGATTAAG TTGGGTAACG CCAGGGTTTT CCCAGTCACG ACGTTGTAAA ACGACGGCCA 181 GTGCCAAGCT GATCTATACA TTGAATCAAT ATTGGCAATT AGCCATATTA GTCATTGGTT 241 ATATAGCATA AATCAATATT GGCTATTGGC CATTGCATAC GTTGTATCTA TATCATAATA 301 TGTACATTTA TATTGGCTCA TGTCCAATAT GACCGCCATG TTGACATTGA TTATTGACTA 361 GTTATTAATA GTAATCAATT ACGGGGTCAT TAGTTCATAG CCCATATATG GAGTTCCGCG 421 TTACATAACT TACGGTAAAT GGCCCGCCTG GCTGACCGCC CAACGACCCC CGCCCATTGA 481 CGTCAATAAT GACGTATGTT CCCATAGTAA CGCCAATAGG GACTTTCCAT TGACGTCAAT 541 GGGTGGAGTA TTTACGGTAA ACTGCCCACT TGGCAGTACA TCAAGTGTAT CATATGCCAA 601 GTCCGCCCCC TATTGACGTC AATGACGGTA AATGGCCCGC CTGGCATTAT GCCCAGTACA 661 TGACCTTACG GGACTTTCCT ACTTGGCAGT ACATCTACGT ATTAGTCATC GCTATTACCA 721 TGGTGATGCG GTTTTGGCAG TACACCAATG GGCGTGGATA GCGGTTTGAC TCACGGGGAT 781 TTCCAAGTCT CCACCCCATT GACGTCAATG GGAGTTTGTT TTGGCACCAA AATCAACGGG 841 ACTTTCCAAA ATGTCGTAAT AACCCCGCCC CGTTGACGCA AATGGGCGGT AGGCGTGTAC 901 GGTGGGAGGT CTATATAAGC AGAGCTCGTT TAGTGAACCG TCAGAATTTT GTAATACGAC 961 TCACTATAGG GCGGCCGGGA ATTCGTCGAC TGGATCCGGT ACCGAGGAGA TCTGCCGCCG 1021 CGATCGCCAT GGCTGTGAGT TTGGATGACG ACGTGCCGCT CATCCTGACC TTGGATGAGG 1081 GTGGCAGTGC CCCACTGGCT CCCTCCAACG GCCTGGGCCA AGAAGAGCTA CCTAGCAAAA 1141 ATGGCGGCAG CTATGCCATC CACGACTCCC AGGCCCCCAG TCTCAGCTCT GGGGGTGAGA 1201 GTTCCCCCTC CAGCCCCGCA CACAACTGGG AGATGAATTA CCAAGAGGCA GCAATCTACC 1261 TCCAGGAAGG CGAGAACAAC GACAAGTTCT TCACCCACCC CAAGGATGCC AAGGCGCTGG 1321 CGGCCTACCT CTTTGCACAC AATCACCTCT TCTACCTGAT GGAGCTGGCC ACGGCCCTGC 1381 TGCTGCTGCT GCTCTCCCTG TGCGAGGCCC CCGCCGTCCC CGCACTCCGG CTTGGCATCT 1441 ATGTCCACGC CACCCTGGAG CTGTTTGCCC TGATGGTGGT AGTGTTTGAA CTCTGCATGA 1501 AGTTACGCTG GCTGGGCCTC CACACCTTCA TCCGGCACAA GCGGACCATG GTCAAGACCT 1561 CGGTGCTGGT GGTGCAGTTT GTCGAGGCCA TCGTGGTGTT GGTACGGCAG ATGTCCCATG 1621 TGCGGGTGAC CCGAGCACTG CGCTGCATTT TCCTGGTGGA CTGTCGGTAT TGCGGTGGCG 1681 TCCGGCGCAA CCTGCGGCAG ATCTTCCAGT CCCTGCCGCC CTTCATGGAC ATCCTCCTGC 1741 TGCTGCTGTT CTTCATGATC ATCTTTGCCA TCCTCGGTTT CTACTTGTTC TCCCCTAACC 1801 CTTCAGACCC CTACTTCAGC ACCCTGGAGA ACAGCATCGT CAGTCTGTTT GTCCTTCTGA 1861 CCACAGCCAA TTTCCCAGAT GTGATGATGC CCTCCTACTC CCGGAACCCC TGGTCCTGCG 1921 TCTTCTTCAT CGTGTACCTC TCCATCGAGC TGTATTTCAT CATGAACCTG CTTCTGGCTG 1981 TGGTGTTCGA CACCTTCAAT GACATTGAGA AACGCAAGTT CAAGTCTTTG CTACTGCACA 2041 AGCGAACCGC TATCCAGCAT GCCTACCGCC TGCTCATCAG CCAGAGGAGG CCTGCCGGCA 2101 TCTCCTACAG GCAGTTTGAA GGCCTCATGC GCTTCTACAA GCCCCGGATG AGTGCCAGGG 2161 AGCGCTATCT TACCTTCAAG GCCCTGAATC AGAACAACAC ACCCCTGCTC AGCCTAAAGG 2221 ACTTTTACGA TATCTACGAA GTTGCTGCTT TGAAGTGGAA GGCCAAGAAA AACAGAGAGC 2281 ACTGGTTTGA TGAGCTTCCC AGGACGGCGC TCCTCATCTT CAAAGGTATT AATATCCTTG 2341 TGAAGTCCAA GGCCTTCCAG TATTTCATGT ACTTGGTGGT GGCAGTCAAC GGGGTCTGGA 2401 TCCTCGTGGA GACATTTATG CTGAAAGGTG GGAACTTCTT CTCCAAGCAC GTGCCCTGGA 2461 GTTACCTCGT CTTTCTAACT ATCTATGGGG TGGAGCTGTT CCTGAAGGTT GCCGGCCTGG 2521 GCCCTGTGGA GTACTTGTCT TCCGGATGGA ACTTGTTTGA CTTCTCCGTG ACAGTGTTCG 2581 CCTTCCTGGG ACTGCTGGCG CTGGCCCTCA ACATGGAGCC CTTCTATTTC ATCGTGGTCC 2641 TGCGCCCCCT CCAGCTGCTG AGGTTGTTTA AGTTGAAGGA GCGCTACCGC AACGTGCTGG 2701 ACACCATGTT CGAGCTGCTG CCCCGGATGG CCAGCCTGGG CCTCACCCTG CTCATCTTTT 2761 ACTACTCCTT CGCCATCGTG GGCATGGAGT TCTTCTGCGG GATCGTCTTC CCCAACTGCT 2821 GCAACACGAG TACAGTGGCA GATGCCTACC GCTGGCGCAA CCACACCGTG GGCAACAGGA 2881 CCGTGGTGGA GGAAGGCTAC TATTATCTCA ATAATTTTGA CAACATCCTC AACAGCTTTG 2941 TGACCCTGTT TGAGCTCACA GTTGTCAACA ACTGGTACAT CATCATGGAA GGCGTCACCT 3001 CTCAGACCTC CCACTGGAGC CGCCTCTACT TCATGACCTT TTACATTGTG ACCATGGTGG 3061 TGATGACGAT CATTGTCGCC TTTATCCTCG AGGCCTTCGT CTTCCGAATG AACTACAGCC 3121 GCAAGAACCA GGACTCGGAA GTTGATGGTG GCATCACCCT TGAGAAGGAA ATCTCCAAAG 3181 AAGAGCTGGT TGCCGTCCTG GAGCTCTACC GGGAGGCACG GGGGGCCTCC TCGGATGTCA 3241 CCAGGCTGCT GGAGACCCTC TCCCAGATGG AGAGATACCA GCAACATTCC ATGGTGTTTC 3301 TGGGACGGCG ATCAAGGACC AAGAGCGACC TGAGCCTGAA GATGTACCAG GAGGAGATCC 3361 AGGAGTGGTA TGAGGAGCAT GCCAGGGAGC AAGAGCAGCA GCGACAACTC AGCAGCAGTG 3421 CAGCCCCCGC CGCCCAGCAG CCCCCAGGCA GCCGCCAGCG CTCCCAGACC GTTACCACGC 3481 GTACGCGGCC GCTCGAGCAG AAACTCATCT CAGAAGAGGA TCTGGCAGCA AATGATATCC 3541 TGGATTACAA GGATGACGAC GATAAGGTTT AAACGGCCGG CCGCGGTCAT AGCTGTTTCC 3601 TGAACAGATC CCGGGTGGCA TCCCTGTGAC CCCTCCCCAG TGCCTCTCCT GGCCCTGGAA 3661 GTTGCCACTC CAGTGCCCAC CAGCCTTGTC CTAATAAAAT TAAGTTGCAT CATTTTGTCT 3721 GACTAGGTGT CCTTCTATAA TATTATGGGG TGGAGGGGGG TGGTATGGAG CAAGGGGCAA 3781 GTTGGGAAGA CAACCTGTAG GGCCTGCGGG GTCTATTGGG AACCAAGCTG GAGTGCAGTG 3841 GCACAATCTT GGCTCACTGC AATCTCCGCC TCCTGGGTTC AAGCGATTCT CCTGCCTCAG 3901 CCTCCCGAGT TGTTGGGATT CCAGGCATGC ATGACCAGGC TCAGCTAATT TTTGTTTTTT 3961 TGGTAGAGAC GGGGTTTCAC CATATTGGCC AGGCTGGTCT CCAACTCCTA ATCTCAGGTG 4021 ATCTACCCAC CTTGGCCTCC CAAATTGCTG GGATTACAGG CGTGAACCAC TGCTCCCTTC 4081 CCTGTCCTTC TGATTTTAAA ATAACTATAC CAGCAGGAGG ACGTCCAGAC ACAGCATAGG 4141 CTACCTGGCC ATGCCCAACC GGTGGGACAT TTGAGTTGCT TGCTTGGCAC TGTCCTCTCA 4201 TGCGTTGGGT CCACTCAGTA GATGCCTGTT GAATTGGGTA CGCGGCCAGC GGCGAGCGGT 4261 ATCAGCTCAC TCAAAGGCGG TAATACGGTT ATCCACAGAA TCAGGGGATA ACGCAGGAAA 4321 GAACATGTGA GCAAAAGGCC AGCAAAAGGC CAGGAACCGT AAAAAGGCCG CGTTGCTGGC 4381 GTTTTTCCAT AGGCTCCGCC CCCCTGACGA GCATCACAAA AATCGACGCT CAAGTCAGAG 4441 GTGGCGAAAC CCGACAGGAC TATAAAGATA CCAGGCGTTT CCCCCTGGAA GCTCCCTCGT 4501 GCGCTCTCCT GTTCCGACCC TGCCGCTTAC CGGATACCTG TCCGCCTTTC TCCCTTCGGG 4561 AAGCGTGGCG CTTTCTCATA GCTCACGCTG TAGGTATCTC AGTTCGGTGT AGGTCGTTCG 4621 CTCCAAGCTG GGCTGTGTGC ACGAACCCCC CGTTCAGCCC GACCGCTGCG CCTTATCCGG 4681 TAACTATCGT CTTGAGTCCA ACCCGGTAAG ACACGACTTA TCGCCACTGG CAGCAGCCAC 4741 TGGTAACAGG ATTAGCAGAG CGAGGTATGT AGGCGGTGCT ACAGAGTTCT TGAAGTGGTG 4801 GCCTAACTAC GGCTACACTA GAAGAACAGT ATTTGGTATC TGCGCTCTGC TGAAGCCAGT 4861 TACCTTCGGA AAAAGAGTTG GTAGCTCTTG ATCCGGCAAA CAAACCACCG CTGGTAGCGG 4921 TGGTTTTTTT GTTTGCAAGC AGCAGATTAC GCGCAGAAAA AAAGGATCTC AAGAAGATCC 4981 TTTGATCTTT TCTACGGGGT CTGACGCTCA GTGGAACGAA AACTCACGTT AAGGGATTTT 5041 GGTCATGAGA TTATCAAAAA GGATCTTCAC CTAGATCCTT TTAAATTAAA AATGAAGTTT 5101 TAAATCAATC TAAAGTATAT ATGAGTAACC TGAGGCTATG GCAGGGCCTG CCGCCCCGAC 5161 GTTGGCTGCG AGCCCTGGGC CTTCACCCGA ACTTGGGGGG TGGGGTGGGG AAAAGGAAGA 5221 AACGCGGGCG TATTGGCCCC AATGGGGTCT CGGTGGGGTA TCGACAGAGT GCCAGCCCTG 5281 GGACCGAACC CCGCGTTTAT GAACAAACGA CCCAACACCG TGCGTTTTAT TCTGTCTTTT 5341 TATTGCCGTC ATAGCGCGGG TTCCTTCCGG TATTGTCTCC TTCCGTGTTT CAGTTAGCCT 5401 CCCCCTAGGG TGGGCGAAGA ACTCCAGCAT GAGATCCCCG CGCTGGAGGA TCATCCAGCC 5461 GGCGTCCCGG AAAACGATTC CGAAGCCCAA CCTTTCATAG AAGGCGGCGG TGGAATCGAA 5521 ATCTCGTGAT GGCAGGTTGG GCGTCGCTTG GTCGGTCATT TCGAACCCCA GAGTCCCGCT 5581 CAGAAGAACT CGTCAAGAAG GCGATAGAAG GCGATGCGCT GCGAATCGGG AGCGGCGATA 5641 CCGTAAAGCA CGAGGAAGCG GTCAGCCCAT TCGCCGCCAA GCTCTTCAGC AATATCACGG 5701 GTAGCCAACG CTATGTCCTG ATAGCGATCC GCCACACCCA GCCGGCCACA GTCGATGAAT 5761 CCAGAAAAGC GGCCATTTTC CACCATGATA TTCGGCAAGC AGGCATCGCC ATGGGTCACG 5821 ACGAGATCCT CGCCGTCGGG CATGCTCGCC TTGAGCCTGG CGAACAGTTC GGCTGGCGCG 5881 AGCCCCTGAT GCTCTTCGTC CAGATCATCC TGATCGACAA GACCGGCTTC CATCCGAGTA 5941 CGTGCTCGCT CGATGCGATG TTTCGCTTGG TGGTCGAATG GGCAGGTAGC CGGATCAAGC 6001 GTATGCAGCC GCCGCATTGC ATCAGCCATG ATGGATACTT TCTCGGCAGG AGCAAGGTGA 6061 GATGACAGGA GATCCTGCCC CGGCACTTCG CCCAATAGCA GCCAGTCCCT TCCCGCTTCA 6121 GTGACAACGT CGAGCACAGC TGCGCAAGGA ACGCCCGTCG TGGCCAGCCA CGATAGCCGC 6181 GCTGCCTCGT CTTGCAGTTC ATTCAGGGCA CCGGACAGGT CGGTCTTGAC AAAAAGAACC 6241 GGGCGCCCCT GCGCTGACAG CCGGAACACG GCGGCATCAG AGCAGCCGAT TGTCTGTTGT 6301 GCCCAGTCAT AGCCGAATAG CCTCTCCACC CAAGCGGCCG GAGAACCTGC GTGCAATCCA 6361 TCTTGTTCAA TCATGCGAAA CGATCCTCAT CCTGTCTCTT GATCGATCTT TGCAAAAGCC 6421 TAGGCCTCCA AAAAAGCCTC CTCACTACTT CTGGAATAGC TCAGAGGCCG AGGCGGCCTC 6481 GGCCTCTGCA TAAATAAAAA AAATTAGTCA GCCATGGGGC GGAGAATGGG CGGAACTGGG 6541 CGGAGTTAGG GGCGGGATGG GCGGAGTTAG GGGCGGGACT ATGGTTGCTG ACTAATTGAG 6601 ATGCATGCTT TGCATACTTC TGCCTGCTGG GGAGCCTGGG GACTTTCCAC ACCTGGTTGC 6661 TGACTAATTG AGATGCATGC TTTGCATACT TCTGCCTGCT GGGGAGCCTG GGGACTTTCC 6721 ACACCCTAAC TGACACACAT TCCACAGCTG GTTCTTTCCG CCTCAGGACT CTTCCTTTTT 6781 CAATATTATT GAAGCATTTA TCAGGGTTAT TGTCTCATGA GCGGATACAT ATTTGAATGT 6841 ATTTAGAAAA ATAAACAAAT AGGGGTTCCG CGCACATTTC CCCGAAAAGT GCCACCTGAC 6901 GCGCCCTGTA GCGGCGCATT AAGCGCGGCG GGTGTGGTGG TTACGCGCAG CGTGACCGCT 6961 ACACTTGCCA GCGCCCTAGC GCCCGCTCCT TTCGCTTTCT TCCCTTCCTT TCTCGCCACG 7021 TTCGCCGGCT TTCCCCGTCA AGCTCTAAAT CGGGGGCTCC CTTTAGGGTT CCGATTTAGT 7081 GCTTTACGGC ACCTCGACCC CAAAAAACTT GATTAGGGTG ATGGTTCACG TAGTGGGCCA 7141 TCGCCCTGAT AGACGGTTTT TCGCCCTTTG ACGTTGGAGT CCACGTTCTT TAATAGTGGA 7201 CTCTTGTTCC AAACTGGAAC AACACTCAAC CCTATCTCGG TCTATTCTTT TGATTTATAA 7261 GGGATTTTGC CGATTTCGGC CTATTGGTTA AAAAATGAGC TGATTTAACA AAAATTTAAC 7321 GCGAATTTT //