LOCUS       RC213184L1                9039 bp    DNA     circular  SYN 
DEFINITION  HMGCR (Myc-DDK-tagged)-Human 3-hydroxy-3-methylglutaryl-CoA reductase (HMGCR), transcript variant 1
ACCESSION   NM_000859
VERSION     null
SOURCE      synthetic construct 
FEATURES    Location/Qualifiers 
     misc_feature    7330..7510 
                     /label="3'LTR"  
     misc_feature    833..1013 
                     /label="5'LTR"  
     misc_feature    8238..8897 
                     /label="CAT(Cam-r)"  
     misc_feature    2620..3234 
                     /label="CMV promoter"  
     misc_feature    7669..8237 
                     /label="ColE1"  
     misc_feature    6205..6225 
                     /label="LR50 primer"  
     misc_feature    6002..6073 
                     /label="Myc-DDK"  
     misc_feature    3252..3271 
                     /label="T7 promoter"  
     misc_feature    3221..3237 
                     /label="V2 primer"  
     misc_feature    6214..6805 
                     /label="WPRE"  
     misc_feature    3320..3328 
                     /label="Restriction Enzyme"  
     misc_feature    5984..5990 
                     /label="Restriction Enzyme"  
     misc_feature    3320..5984 
                     /label="HMGCR"  
ORIGIN                      
        1 GTCGACGGAT CGGGAGATCT CCCGATCCCC TATGGTGCAC TCTCAGTACA ATCTGCTCTG 
       61 ATGCCGCATA GTTAAGCCAG TATCTGCTCC CTGCTTGTGT GTTGGAGGTC GCTGAGTAGT 
      121 GCGCGAGCAA AATTTAAGCT ACAACAAGGC AAGGCTTGAC CGACAATTGC ATGAAGAATC 
      181 TGCTTAGGGT TAGGCGTTTT GCGCTGCTTC GCGATGTACG GGCCAGATAT CGCGTTGACA 
      241 TTGATTATTG ACTAGTTATT AATAGTAATC AATTACGGGG TCATTAGTTC ATAGCCCATA 
      301 TATGGAGTTC CGCGTTACAT AACTTACGGT AAATGGCCCG CCTGGCTGAC CGCCCAACGA 
      361 CCCCCGCCCA TTGACGTCAA TAATGACGTA TGTTCCCATA GTAACGCCAA TAGGGACTTT 
      421 CCATTGACGT CAATGGGTGG AGTATTTACG GTAAACTGCC CACTTGGCAG TACATCAAGT 
      481 GTATCATATG CCAAGTACGC CCCCTATTGA CGTCAATGAC GGTAAATGGC CCGCCTGGCA 
      541 TTATGCCCAG TACATGACCT TATGGGACTT TCCTACTTGG CAGTACATCT ACGTATTAGT 
      601 CATCGCTATT ACCATGGTGA TGCGGTTTTG GCAGTACATC AATGGGCGTG GATAGCGGTT 
      661 TGACTCACGG GGATTTCCAA GTCTCCACCC CATTGACGTC AATGGGAGTT TGTTTTGGCA 
      721 CCAAAATCAA CGGGACTTTC CAAAATGTCG TAACAACTCC GCCCCATTGA CGCAAATGGG 
      781 CGGTAGGCGT GTACGGTGGG AGGTCTATAT AAGCAGCGCG TTTTGCCTGT ACTGGGTCTC 
      841 TCTGGTTAGA CCAGATCTGA GCCTGGGAGC TCTCTGGCTA ACTAGGGAAC CCACTGCTTA 
      901 AGCCTCAATA AAGCTTGCCT TGAGTGCTTC AAGTAGTGTG TGCCCGTCTG TTGTGTGACT 
      961 CTGGTAACTA GAGATCCCTC AGACCCTTTT AGTCAGTGTG GAAAATCTCT AGCAGTGGCG 
     1021 CCCGAACAGG GACTTGAAAG CGAAAGGGAA ACCAGAGGAG CTCTCTCGAC GCAGGACTCG 
     1081 GCTTGCTGAA GCGCGCACGG CAAGAGGCGA GGGGCGGCGA CTGGTGAGTA CGCCAAAAAT 
     1141 TTTGACTAGC GGAGGCTAGA AGGAGAGAGA TGGGTGCGAG AGCGTCAGTA TTAAGCGGGG 
     1201 GAGAATTAGA TCGCGATGGG AAAAAATTCG GTTAAGGCCA GGGGGAAAGA AAAAATATAA 
     1261 ATTAAAACAT ATAGTATGGG CAAGCAGGGA GCTAGAACGA TTCGCAGTTA ATCCTGGCCT 
     1321 GTTAGAAACA TCAGAAGGCT GTAGACAAAT ACTGGGACAG CTACAACCAT CCCTTCAGAC 
     1381 AGGATCAGAA GAACTTAGAT CATTATATAA TACAGTAGCA ACCCTCTATT GTGTGCATCA 
     1441 AAGGATAGAG ATAAAAGACA CCAAGGAAGC TTTAGACAAG ATAGAGGAAG AGCAAAACAA 
     1501 AAGTAAGACC ACCGCACAGC AAGCGGCCGG CCGCTGATCT TCAGACCTGG AGGAGGAGAT 
     1561 ATGAGGGACA ATTGGAGAAG TGAATTATAT AAATATAAAG TAGTAAAAAT TGAACCATTA 
     1621 GGAGTAGCAC CCACCAAGGC AAAGAGAAGA GTGGTGCAGA GAGAAAAAAG AGCAGTGGGA 
     1681 ATAGGAGCTT TGTTCCTTGG GTTCTTGGGA GCAGCAGGAA GCACTATGGG CGCAGCGTCA 
     1741 ATGACGCTGA CGGTACAGGC CAGACAATTA TTGTCTGGTA TAGTGCAGCA GCAGAACAAT 
     1801 TTGCTGAGGG CTATTGAGGC GCAACAGCAT CTGTTGCAAC TCACAGTCTG GGGCATCAAG 
     1861 CAGCTCCAGG CAAGAATCCT GGCTGTGGAA AGATACCTAA AGGATCAACA GCTCCTGGGG 
     1921 ATTTGGGGTT GCTCTGGAAA ACTCATTTGC ACCACTGCTG TGCCTTGGAA TGCTAGTTGG 
     1981 AGTAATAAAT CTCTGGAACA GATTTGGAAT CACACGACCT GGATGGAGTG GGACAGAGAA 
     2041 ATTAACAATT ACACAAGCTT AATACACTCC TTAATTGAAG AATCGCAAAA CCAGCAAGAA 
     2101 AAGAATGAAC AAGAATTATT GGAATTAGAT AAATGGGCAA GTTTGTGGAA TTGGTTTAAC 
     2161 ATAACAAATT GGCTGTGGTA TATAAAATTA TTCATAATGA TAGTAGGAGG CTTGGTAGGT 
     2221 TTAAGAATAG TTTTTGCTGT ACTTTCTATA GTGAATAGAG TTAGGCAGGG ATATTCACCA 
     2281 TTATCGTTTC AGACCCACCT CCCAACCCCG AGGGGACCCG ACAGGCCCGA AGGAATAGAA 
     2341 GAAGAAGGTG GAGAGAGAGA CAGAGACAGA TCCATTCGAT TAGTGAACGG ATCGGCACTG 
     2401 CGTGCGCCAA TTCTGCAGAC AAATGGCAGT ATTCATCCAC AATTTTAAAA GAAAAGGGGG 
     2461 GATTGGGGGG TACAGTGCAG GGGAAAGAAT AGTAGACATA ATAGCAACAG ACATACAAAC 
     2521 TAAAGAATTA CAAAAACAAA TTACAAAAAT TCAAAATTTT CGGGTTTATT ACAGGGACAG 
     2581 CAGAGATCCA GTTTGGTTAG TACCGGGCCC GCTCTAGACA TGTCCAATAT GACCGCCATG 
     2641 TTGACATTGA TTATTGACTA GTTATTAATA GTAATCAATT ACGGGGTCAT TAGTTCATAG 
     2701 CCCATATATG GAGTTCCGCG TTACATAACT TACGGTAAAT GGCCCGCCTG GCTGACCGCC 
     2761 CAACGACCCC CGCCCATTGA CGTCAATAAT GACGTATGTT CCCATAGTAA CGCCAATAGG 
     2821 GACTTTCCAT TGACGTCAAT GGGTGGAGTA TTTACGGTAA ACTGCCCACT TGGCAGTACA 
     2881 TCAAGTGTAT CATATGCCAA GTCCGCCCCC TATTGACGTC AATGACGGTA AATGGCCCGC 
     2941 CTGGCATTAT GCCCAGTACA TGACCTTACG GGACTTTCCT ACTTGGCAGT ACATCTACGT 
     3001 ATTAGTCATC GCTATTACCA TGGTGATGCG GTTTTGGCAG TACACCAATG GGCGTGGATA 
     3061 GCGGTTTGAC TCACGGGGAT TTCCAAGTCT CCACCCCATT GACGTCAATG GGAGTTTGTT 
     3121 TTGGCACCAA AATCAACGGG ACTTTCCAAA ATGTCGTAAT AACCCCGCCC CGTTGACGCA 
     3181 AATGGGCGGT AGGCGTGTAC GGTGGGAGGT CTATATAAGC AGAGCTCGTT TAGTGAACCG 
     3241 TCAGAATTTT GTAATACGAC TCACTATAGG GCGGCCGGGA ATTCGTCGAC TGGATCCGGT 
     3301 ACCGAGGAGA TCTGCCGCCG CGATCGCCAT GTTGTCAAGA CTTTTTCGAA TGCATGGCCT 
     3361 CTTTGTGGCC TCCCATCCCT GGGAAGTCAT AGTGGGGACA GTGACACTGA CCATCTGCAT 
     3421 GATGTCCATG AACATGTTTA CTGGTAACAA TAAGATCTGT GGTTGGAATT ATGAATGTCC 
     3481 AAAGTTTGAA GAGGATGTTT TGAGCAGTGA CATTATAATT CTGACAATAA CACGATGCAT 
     3541 AGCCATCCTG TATATTTACT TCCAGTTCCA GAATTTACGT CAACTTGGAT CAAAATATAT 
     3601 TTTGGGTATT GCTGGCCTTT TCACAATTTT CTCAAGTTTT GTATTCAGTA CAGTTGTCAT 
     3661 TCACTTCTTA GACAAAGAAT TGACAGGCTT GAATGAAGCT TTGCCCTTTT TCCTACTTTT 
     3721 GATTGACCTT TCCAGAGCAA GCACATTAGC AAAGTTTGCC CTCAGTTCCA ACTCACAGGA 
     3781 TGAAGTAAGG GAAAATATTG CTCGTGGAAT GGCAATTTTA GGTCCTACGT TTACCCTCGA 
     3841 TGCTCTTGTT GAATGTCTTG TGATTGGAGT TGGTACCATG TCAGGGGTAC GTCAGCTTGA 
     3901 AATTATGTGC TGCTTTGGCT GCATGTCAGT TCTTGCCAAC TACTTCGTGT TCATGACTTT 
     3961 CTTCCCAGCT TGTGTGTCCT TGGTATTAGA GCTTTCTCGG GAAAGCCGCG AGGGTCGTCC 
     4021 AATTTGGCAG CTCAGCCATT TTGCCCGAGT TTTAGAAGAA GAAGAAAATA AGCCGAATCC 
     4081 TGTAACTCAG AGGGTCAAGA TGATTATGTC TCTAGGCTTG GTTCTTGTTC ATGCTCACAG 
     4141 TCGCTGGATA GCTGATCCTT CTCCTCAAAA CAGTACAGCA GATACTTCTA AGGTTTCATT 
     4201 AGGACTGGAT GAAAATGTGT CCAAGAGAAT TGAACCAAGT GTTTCCCTCT GGCAGTTTTA 
     4261 TCTCTCTAAA ATGATCAGCA TGGATATTGA ACAAGTTATT ACCCTAAGTT TAGCTCTCCT 
     4321 TCTGGCTGTC AAGTACATCT TCTTTGAACA AACAGAGACA GAATCTACAC TCTCATTAAA 
     4381 AAACCCTATC ACATCTCCTG TAGTGACACA AAAGAAAGTC CCAGACAATT GTTGTAGACG 
     4441 TGAACCTATG CTGGTCAGAA ATAACCAGAA ATGTGATTCA GTAGAGGAAG AGACAGGGAT 
     4501 AAACCGAGAA AGAAAAGTTG AGGTTATAAA ACCCTTAGTG GCTGAAACAG ATACCCCAAA 
     4561 CAGAGCTACA TTTGTGGTTG GTAACTCCTC CTTACTCGAT ACTTCATCAG TACTGGTGAC 
     4621 ACAGGAACCT GAAATTGAAC TTCCCAGGGA ACCTCGGCCT AATGAAGAAT GTCTACAGAT 
     4681 ACTTGGGAAT GCAGAGAAAG GTGCAAAATT CCTTAGTGAT GCTGAGATCA TCCAGTTAGT 
     4741 CAATGCTAAG CATATCCCAG CCTACAAGTT GGAAACTCTG ATGGAAACTC ATGAGCGTGG 
     4801 TGTATCTATT CGCCGACAGT TACTTTCCAA GAAGCTTTCA GAACCTTCTT CTCTCCAGTA 
     4861 CCTACCTTAC AGGGATTATA ATTACTCCTT GGTGATGGGA GCTTGTTGTG AGAATGTTAT 
     4921 TGGATATATG CCCATCCCTG TTGGAGTGGC AGGACCCCTT TGCTTAGATG AAAAAGAATT 
     4981 TCAGGTTCCA ATGGCAACAA CAGAAGGTTG TCTTGTGGCC AGCACCAATA GAGGCTGCAG 
     5041 AGCAATAGGT CTTGGTGGAG GTGCCAGCAG CCGAGTCCTT GCAGATGGGA TGACTCGTGG 
     5101 CCCAGTTGTG CGTCTTCCAC GTGCTTGTGA CTCTGCAGAA GTGAAAGCCT GGCTCGAAAC 
     5161 ATCTGAAGGG TTCGCAGTGA TAAAGGAGGC ATTTGACAGC ACTAGCAGAT TTGCACGTCT 
     5221 ACAGAAACTT CATACAAGTA TAGCTGGACG CAACCTTTAT ATCCGTTTCC AGTCCAGGTC 
     5281 AGGGGATGCC ATGGGGATGA ACATGATTTC AAAGGGTACA GAGAAAGCAC TTTCAAAACT 
     5341 TCACGAGTAT TTCCCTGAAA TGCAGATTCT AGCCGTTAGT GGTAACTATT GTACTGACAA 
     5401 GAAACCTGCT GCTATAAATT GGATAGAGGG AAGAGGAAAA TCTGTTGTTT GTGAAGCTGT 
     5461 CATTCCAGCC AAGGTTGTCA GAGAAGTATT AAAGACTACC ACAGAGGCTA TGATTGAGGT 
     5521 CAACATTAAC AAGAATTTAG TGGGCTCTGC CATGGCTGGG AGCATAGGAG GCTACAACGC 
     5581 CCATGCAGCA AACATTGTCA CCGCCATCTA CATTGCCTGT GGACAGGATG CAGCACAGAA 
     5641 TGTTGGTAGT TCAAACTGTA TTACTTTAAT GGAAGCAAGT GGTCCCACAA ATGAAGATTT 
     5701 ATATATCAGC TGCACCATGC CATCTATAGA GATAGGAACG GTGGGTGGTG GGACCAACCT 
     5761 ACTACCTCAG CAAGCCTGTT TGCAGATGCT AGGTGTTCAA GGAGCATGCA AAGATAATCC 
     5821 TGGGGAAAAT GCCCGGCAGC TTGCCCGAAT TGTGTGTGGG ACCGTAATGG CTGGGGAATT 
     5881 GTCACTTATG GCAGCATTGG CAGCAGGACA TCTTGTCAAA AGTCACATGA TTCACAACAG 
     5941 GTCGAAGATC AATTTACAAG ACCTCCAAGG AGCTTGCACC AAGAAGACAG CCACGCGTAC 
     6001 GCGGCCGCTC GAGCAGAAAC TCATCTCAGA AGAGGATCTG GCAGCAAATG ATATCCTGGA 
     6061 TTACAAGGAT GACGACGATA AGGTTTAAAC GGCCGGCCGC GGTCTGTACA AGTAGGATTC 
     6121 GTCGAGGGAC CTAATAACTT CGTATAGCAT ACATTATACG AAGTTATACA TGTTTAAGGG 
     6181 TTCCGGTTCC ACTAGGTACA ATTCGATATC AAGCTTATCG ATAATCAACC TCTGGATTAC 
     6241 AAAATTTGTG AAAGATTGAC TGGTATTCTT AACTATGTTG CTCCTTTTAC GCTATGTGGA 
     6301 TACGCTGCTT TAATGCCTTT GTATCATGCT ATTGCTTCCC GTATGGCTTT CATTTTCTCC 
     6361 TCCTTGTATA AATCCTGGTT GCTGTCTCTT TATGAGGAGT TGTGGCCCGT TGTCAGGCAA 
     6421 CGTGGCGTGG TGTGCACTGT GTTTGCTGAC GCAACCCCCA CTGGTTGGGG CATTGCCACC 
     6481 ACCTGTCAGC TCCTTTCCGG GACTTTCGCT TTCCCCCTCC CTATTGCCAC GGCGGAACTC 
     6541 ATCGCCGCCT GCCTTGCCCG CTGCTGGACA GGGGCTCGGC TGTTGGGCAC TGACAATTCC 
     6601 GTGGTGTTGT CGGGGAAATC ATCGTCCTTT CCTTGGCTGC TCGCCTGTGT TGCCACCTGG 
     6661 ATTCTGCGCG GGACGTCCTT CTGCTACGTC CCTTCGGCCC TCAATCCAGC GGACCTTCCT 
     6721 TCCCGCGGCC TGCTGCCGGC TCTGCGGCCT CTTCCGCGTC TTCGCCTTCG CCCTCAGACG 
     6781 AGTCGGATCT CCCTTTGGGC CGCCTCCCCG CATCGATACC GTCGACCTCG ATCGAGACCT 
     6841 AGAAAAACAT GGAGCAATCA CAAGTAGCAA TACAGCAGCT ACCAATGCTG ATTGTGCCTG 
     6901 GCTAGAAGCA CAAGAGGAGG AGGAGGTGGG TTTTCCAGTC ACACCTCAGG TACCTTTAAG 
     6961 ACCAATGACT TACAAGGCAG CTGTAGATCT TAGCCACTTT TTAAAAGAAA AGGGGGGACT 
     7021 GGAAGGGCTA ATTCACTCCC AACGAAGACA AGATATCCTT GATCTGTGGA TCTACCACAC 
     7081 ACAAGGCTAC TTCCCTGATT GGCAGAACTA CACACCAGGG CCAGGGATCA GATATCCACT 
     7141 GACCTTTGGA TGGTGCTACA AGCTAGTACC AGTTGAGCAA GAGAAGGTAG AAGAAGCCAA 
     7201 TGAAGGAGAG AACACCCGCT TGTTACACCC TGTGAGCCTG CATGGGATGG ATGACCCGGA 
     7261 GAGAGAAGTA TTAGAGTGGA GGTTTGACAG CCGCCTAGCA TTTCATCACA TGGCCCGAGA 
     7321 GCTGCATCCG GACTGTACTG GGTCTCTCTG GTTAGACCAG ATCTGAGCCT GGGAGCTCTC 
     7381 TGGCTAACTA GGGAACCCAC TGCTTAAGCC TCAATAAAGC TTGCCTTGAG TGCTTCAAGT 
     7441 AGTGTGTGCC CGTCTGTTGT GTGACTCTGG TAACTAGAGA TCCCTCAGAC CCTTTTAGTC 
     7501 AGTGTGGAAA ATCTCTAGCA GCATGTGAGC AAAAGGCCAG CAAAAGGCCA GGAACCGTAA 
     7561 AAAGGCCGCG TTGCTGGCGT TTTTCCATAG GCTCCGCCCC CCTGACGAGC ATCACAAAAA 
     7621 TCGACGCTCA AGTCAGAGGT GGCGAAACCC GACAGGACTA TAAAGATACC AGGCGTTTCC 
     7681 CCCTGGAAGC TCCCTCGTGC GCTCTCCTGT TCCGACCCTG CCGCTTACCG GATACCTGTC 
     7741 CGCCTTTCTC CCTTCGGGAA GCGTGGCGCT TTCTCATAGC TCACGCTGTA GGTATCTCAG 
     7801 TTCGGTGTAG GTCGTTCGCT CCAAGCTGGG CTGTGTGCAC GAACCCCCCG TTCAGCCCGA 
     7861 CCGCTGCGCC TTATCCGGTA ACTATCGTCT TGAGTCCAAC CCGGTAAGAC ACGACTTATC 
     7921 GCCACTGGCA GCAGCCACTG GTAACAGGAT TAGCAGAGCG AGGTATGTAG GCGGTGCTAC 
     7981 AGAGTTCTTG AAGTGGTGGC CTAACTACGG CTACACTAGA AGAACAGTAT TTGGTATCTG 
     8041 CGCTCTGCTG AAGCCAGTTA CCTTCGGAAA AAGAGTTGGT AGCTCTTGAT CCGGCAAACA 
     8101 AACCACCGCT GGTAGCGGTG GTTTTTTTGT TTGCAAGCAG CAGATTACGC GCAGAAAAAA 
     8161 AGGATCTCAA GAAGATCCTT TGATCTTTTC TACGGGGTCT GACGCTCAGT GGAACGAAAA 
     8221 CTCACGTTAA GGGATTTTGG TCATGATTAC GCCCCGCCCT GCCACTCATC GCAGTACTGT 
     8281 TGTAATTCAT TAAGCATTCT GCCGACATGG AAGCCATCAC AAACGGCATG ATGAACCTGA 
     8341 ATCGCCAGCG GCATCAGCAC CTTGTCGCCT TGCGTATAAT ATTTGCCCAT GGTGAAAACG 
     8401 GGGGCGAAGA AGTTGTCCAT ATTGGCCACG TTTAAATCAA AACTGGTGAA ACTCACCCAG 
     8461 GGATTGGCTG AGACGAAAAA CATATTCTCA ATAAACCCTT TAGGGAAATA GGCCAGGTTT 
     8521 TCACCGTAAC ACGCCACATC TTGCGAATAT ATGTGTAGAA ACTGCCGGAA ATCGTCGTGG 
     8581 TATTCACTCC AGAGCGATGA AAACGTTTCA GTTTGCTCAT GGAAAACGGT GTAACAAGGG 
     8641 TGAACACTAT CCCATATCAC CAGCTCACCG TCTTTCATTG CCATACGGAA CTCCGGATGA 
     8701 GCATTCATCA GGCGGGCAAG AATGTGAATA AAGGCCGGAT AAAACTTGTG CTTATTTTTC 
     8761 TTTACGGTCT TTAAAAAGGC CGTAATATCC AGCTGAACGG TCTGGTTATA GGTACATTGA 
     8821 GCAACTGACT GAAATGCCTC AAAATGTTCT TTACGATGCC ATTGGGATAT ATCAACGGTG 
     8881 GTATATCCAG TGATTTTTTT CTCCATACTC TTCCTTTTTC AATATTATTG AAGCATTTAT 
     8941 CAGGGTTATT GTCTCATGAG CGGATACATA TTTGAATGTA TTTAGAAAAA TAAACAAATA 
     9001 GGGGTCCCGC GCACATTTCC CCGAAAAGTG CCACCTGAC
//