LOCUS       RC213159                8478 bp    DNA     circular  SYN 
DEFINITION  TAF2 (Myc-DDK-tagged)-Human TAF2 RNA polymerase II, TATA box binding protein (TBP)-associated factor, 150kDa (TAF2)
ACCESSION   NM_003184
VERSION     NM_003184.2
SOURCE      synthetic construct 
FEATURES    Location/Qualifiers 
     misc_feature    201..924 
                     /label="CMV promoter"  
     misc_feature    839..856 
                     /label="Restriction Enzyme"  
     misc_feature    953..971 
                     /label="T7 promoter"  
     misc_feature    4635..4726 
                     /label="Myc-DDK"  
     misc_feature    4756..5341 
                     /label="PolyA signal"  
     misc_feature    4813..4832 
                     /label="Restriction Enzyme"  
     misc_feature    5617..6289 
                     /label="ColE1"  
     misc_feature    6719..7513 
                     /label="Kan/Neo"  
     misc_feature    7548..7850 
                     /label="SV40 ori"  
     misc_feature    7936..8413 
                     /label="f1 ori"  
     misc_feature    1020..1028 
                     /label="Restriction Enzyme"  
     misc_feature    4617..4623 
                     /label="Restriction Enzyme"  
     misc_feature    1020..4617 
                     /label="TAF2"  
ORIGIN                      
        1 AACAAAATAT TAACGCTTAC AATTTCCATT CGCCATTCAG GCTGCGCAAC TGTTGGGAAG 
       61 GGCGATCGGT GCGGGCCTCT TCGCTATTAC GCCAGCTGGC GAAAGGGGGA TGTGCTGCAA 
      121 GGCGATTAAG TTGGGTAACG CCAGGGTTTT CCCAGTCACG ACGTTGTAAA ACGACGGCCA 
      181 GTGCCAAGCT GATCTATACA TTGAATCAAT ATTGGCAATT AGCCATATTA GTCATTGGTT 
      241 ATATAGCATA AATCAATATT GGCTATTGGC CATTGCATAC GTTGTATCTA TATCATAATA 
      301 TGTACATTTA TATTGGCTCA TGTCCAATAT GACCGCCATG TTGACATTGA TTATTGACTA 
      361 GTTATTAATA GTAATCAATT ACGGGGTCAT TAGTTCATAG CCCATATATG GAGTTCCGCG 
      421 TTACATAACT TACGGTAAAT GGCCCGCCTG GCTGACCGCC CAACGACCCC CGCCCATTGA 
      481 CGTCAATAAT GACGTATGTT CCCATAGTAA CGCCAATAGG GACTTTCCAT TGACGTCAAT 
      541 GGGTGGAGTA TTTACGGTAA ACTGCCCACT TGGCAGTACA TCAAGTGTAT CATATGCCAA 
      601 GTCCGCCCCC TATTGACGTC AATGACGGTA AATGGCCCGC CTGGCATTAT GCCCAGTACA 
      661 TGACCTTACG GGACTTTCCT ACTTGGCAGT ACATCTACGT ATTAGTCATC GCTATTACCA 
      721 TGGTGATGCG GTTTTGGCAG TACACCAATG GGCGTGGATA GCGGTTTGAC TCACGGGGAT 
      781 TTCCAAGTCT CCACCCCATT GACGTCAATG GGAGTTTGTT TTGGCACCAA AATCAACGGG 
      841 ACTTTCCAAA ATGTCGTAAT AACCCCGCCC CGTTGACGCA AATGGGCGGT AGGCGTGTAC 
      901 GGTGGGAGGT CTATATAAGC AGAGCTCGTT TAGTGAACCG TCAGAATTTT GTAATACGAC 
      961 TCACTATAGG GCGGCCGGGA ATTCGTCGAC TGGATCCGGT ACCGAGGAGA TCTGCCGCCG 
     1021 CGATCGCCAT GCCGCTGACT GGTGTAGAGC CCGCCAGAAT GAACAGGAAG AAAGGAGACA 
     1081 AGGGCTTTGA AAGCCCAAGG CCATATAAAT TAACCCATCA GGTCGTCTGC ATCAACAACA 
     1141 TAAATTTCCA GAGAAAATCT GTTGTGGGAT TTGTGGAACT GACTATATTT CCCACAGTTG 
     1201 CAAACTTGAA TAGAATCAAG TTGAACAGCA AACAGTGTAG AATATACCGA GTAAGGATCA 
     1261 ATGATTTAGA GGCTGCTTTT ATTTATAATG ACCCAACCTT GGAAGTTTGT CACAGTGAAT 
     1321 CAAAACAGAG AAACCTCAAT TATTTTTCCA ATGCTTATGC AGCTGCAGTT AGTGCTGTGG 
     1381 ACCCTGATGC AGGAAATGGA GAACTTTGCA TTAAGGTTCC ATCAGAGCTA TGGAAACACG 
     1441 TTGATGAGTT AAAGGTCCTG AAGATACACA TCAATTTTTC TTTGGATCAG CCCAAAGGAG 
     1501 GTCTTCATTT TGTGGTACCC AGTGTAGAGG GAAGTATGGC AGAGAGAGGT GCTCATGTTT 
     1561 TCTCTTGTGG GTATCAAAAT TCTACAAGAT TTTGGTTCCC TTGTGTTGAT TCATACTCTG 
     1621 AATTGTGTAC ATGGAAATTA GAATTTACAG TAGATGCTGC AATGGTTGCT GTTTCTAATG 
     1681 GCGATTTGGT GGAGACAGTG TATACTCATG ATATGAGGAA GAAAACTTTC CATTATATGC 
     1741 TTACCATTCC TACAGCAGCG TCAAATATCT CCTTGGCCAT TGGACCATTT GAAATACTGG 
     1801 TAGATCCATA CATGCATGAG GTTACTCATT TTTGTTTGCC CCAACTTCTT CCATTGCTGA 
     1861 AACATACCAC ATCATACCTT CATGAAGTCT TTGAATTTTA TGAAGAAATT CTTACATGTC 
     1921 GTTACCCATA CTCCTGTTTT AAGACTGTCT TCATTGATGA GGCTTATGTT GAAGTGGCTG 
     1981 CTTATGCTTC CATGAGCATT TTTAGCACAA ATCTTTTACA CAGTGCCATG ATTATAGATG 
     2041 AGACACCTTT GACTAGAAGG TGTTTAGCCC AATCCTTGGC CCAGCAGTTT TTTGGTTGTT 
     2101 TCATATCTAG AATGTCTTGG TCTGATGAAT GGGTGCTGAA GGGAATTTCA GGCTATATCT 
     2161 ATGGACTTTG GATGAAAAAA ACTTTTGGTG TTAATGAGTA CCGCCATTGG ATTAAAGAGG 
     2221 AGCTAGACAA AATAGTGGCA TATGAACTAA AAACTGGTGG GGTTTTACTA CATCCCATAT 
     2281 TTGGTGGAGG AAAAGAGAAG GATAATCCGG CTTCCCATCT ACACTTTTCA ATAAAGCATC 
     2341 CACATACACT GTCCTGGGAA TACTACACTA TGTTTCAGTG TAAAGCCCAC CTTGTGATGA 
     2401 GATTGATTGA AAATAGGATC AGTATGGAAT TTATGCTACA AGTTTTCAAT AAACTGCTAA 
     2461 GTCTGGCTAG TACTGCTTCA TCTCAGAAGT TCCAGTCACA TATGTGGAGT CAGATGTTGG 
     2521 TTTCCACATC TGGGTTTTTG AAATCCATTT CAAATGTCTC TGGCAAAGAT ATTCAGCCGT 
     2581 TAATAAAGCA GTGGGTAGAT CAGAGTGGAG TGGTAAAATT TTATGGAAGT TTTGCATTTA 
     2641 ATAGAAAACG AAATGTCTTG GAACTGGAAA TAAAACAGGA CTATACATCT CCTGGAACTC 
     2701 AGAAATACGT GGGACCACTT AAAGTGACAG TGCAGGAGTT AGATGGATCC TTCAATCATA 
     2761 CACTGCAAAT TGAAGAAAAC AGCCTTAAAC ATGATATACC CTGCCATTCC AAAAGTAGAA 
     2821 GGAATAAAAA GAAAAAAATC CCACTGATGA ATGGAGAAGA AGTTGATATG GATCTTTCTG 
     2881 CAATGGATGC TGATTCCCCT TTGCTGTGGA TAAGGATAGA CCCAGATATG TCAGTATTGA 
     2941 GGAAGGTAGA ATTTGAGCAA GCTGATTTTA TGTGGCAGTA TCAGCTCCGC TATGAGAGAG 
     3001 ATGTTGTTGC ACAGCAGGAA TCCATTTTGG CTTTGGAAAA ATTCCCTACT CCAGCATCTC 
     3061 GGCTTGCACT CACTGATATA TTAGAACAAG AGCAGTGTTT CTACAGAGTA AGAATGTCAG 
     3121 CTTGCTTCTG TCTTGCAAAG ATTGCAAATT CAATGGTGAG CACATGGACA GGACCACCAG 
     3181 CCATGAAGTC ACTCTTCACT AGGATGTTTT GTTGTAAAAG TTGTCCAAAC ATTGTGAAAA 
     3241 CAAACAACTT TATGAGCTTT CAAAGCTATT TTCTACAGAA GACTATGCCA GTTGCAATGG 
     3301 CTTTATTAAG AGATGTTCAT AATCTTTGTC CTAAAGAAGT CTTAACGTTT ATTTTAGACT 
     3361 TAATCAAGTA CAATGACAAC AGGAAAAATA AGTTTTCAGA TAACTATTAT CGTGCAGAAA 
     3421 TGATTGATGC CCTGGCCAAC TCTGTTACAC CTGCAGTCAG TGTGAATAAT GAAGTTAGAA 
     3481 CTTTGGATAA CTTAAATCCT GATGTGCGAC TCATTCTTGA AGAAATCACC AGATTTTTGA 
     3541 ATATGGAAAA ACTTCTTCCG AGTTACAGGC ATACCATCAC TGTCAGTTGT TTGAGAGCCA 
     3601 TACGGGTACT TCAGAAGAAC GGACATGTGC CAAGTGATCC AGCTCTTTTT AAATCTTATG 
     3661 CTGAATATGG CCACTTTGTG GACATTAGGA TAGCAGCTTT GGAAGCAGTT GTTGATTATA 
     3721 CTAAAGTGGA CAGAAGTTAT GAAGAACTGC AATGGCTACT TAATATGATT CAGAATGACC 
     3781 CTGTACCCTA TGTAAGGCAT AAGATTCTCA ACATGTTGAC TAAGAACCCA CCATTTACTA 
     3841 AGAACATGGA GTCTCCCTTA TGCAATGAAG CCCTGGTAGA TCAACTTTGG AAACTTATGA 
     3901 ATTCTGGTAC TTCACATGAC TGGAGGTTAC GGTGTGGTGC TGTGGACTTG TACTTCACAC 
     3961 TTTTTGGCCT CAGTAGACCT TCCTGTTTAC CCTTGCCAGA GCTTGGGTTG GTTCTTAATC 
     4021 TAAAGGAGAA AAAAGCTGTC TTGAATCCTA CCATAATTCC AGAGTCAGTA GCAGGCAACC 
     4081 AAGAAGCTGC AAATAATCCA AGCAGTCACC CACAGCTAGT TGGATTTCAG AACCCTTTTT 
     4141 CCAGTTCTCA AGATGAGGAG GAGATTGATA TGGATACTGT TCATGATAGC CAGGCCTTCA 
     4201 TTTCCCATCA TTTAAACATG CTTGAAAGGC CGTCAACTCC AGGGCTCTCG AAATATCGGC 
     4261 CAGCTAGCTC CCGATCTGCT TTAATACCCC AGCACTCAGC AGGCTGTGAC AGCACACCCA 
     4321 CCACAAAACC CCAGTGGAGT TTGGAACTTG CACGGAAGGG AACAGGTAAA GAACAAGCAC 
     4381 CTTTGGAGAT GAGTATGCAT CCAGCGGCAA GCGCTCCACT CTCAGTCTTT ACTAAGGAAT 
     4441 CTACAGCCTC CAAACACAGT GACCACCATC ACCACCATCA CCATGAGCAC AAGAAAAAGA 
     4501 AGAAGAAGCA TAAACATAAG CACAAACACA AGCATAAGCA TGACAGTAAA GAAAAGGACA 
     4561 AGGAGCCTTT CACTTTCTCC AGCCCTGCCA GTGGCAGGTC TATTCGTTCT CCTTCCCTTT 
     4621 CAGACACGCG TACGCGGCCG CTCGAGCAGA AACTCATCTC AGAAGAGGAT CTGGCAGCAA 
     4681 ATGATATCCT GGATTACAAG GATGACGACG ATAAGGTTTA AACGGCCGGC CGCGGTCATA 
     4741 GCTGTTTCCT GAACAGATCC CGGGTGGCAT CCCTGTGACC CCTCCCCAGT GCCTCTCCTG 
     4801 GCCCTGGAAG TTGCCACTCC AGTGCCCACC AGCCTTGTCC TAATAAAATT AAGTTGCATC 
     4861 ATTTTGTCTG ACTAGGTGTC CTTCTATAAT ATTATGGGGT GGAGGGGGGT GGTATGGAGC 
     4921 AAGGGGCAAG TTGGGAAGAC AACCTGTAGG GCCTGCGGGG TCTATTGGGA ACCAAGCTGG 
     4981 AGTGCAGTGG CACAATCTTG GCTCACTGCA ATCTCCGCCT CCTGGGTTCA AGCGATTCTC 
     5041 CTGCCTCAGC CTCCCGAGTT GTTGGGATTC CAGGCATGCA TGACCAGGCT CAGCTAATTT 
     5101 TTGTTTTTTT GGTAGAGACG GGGTTTCACC ATATTGGCCA GGCTGGTCTC CAACTCCTAA 
     5161 TCTCAGGTGA TCTACCCACC TTGGCCTCCC AAATTGCTGG GATTACAGGC GTGAACCACT 
     5221 GCTCCCTTCC CTGTCCTTCT GATTTTAAAA TAACTATACC AGCAGGAGGA CGTCCAGACA 
     5281 CAGCATAGGC TACCTGGCCA TGCCCAACCG GTGGGACATT TGAGTTGCTT GCTTGGCACT 
     5341 GTCCTCTCAT GCGTTGGGTC CACTCAGTAG ATGCCTGTTG AATTGGGTAC GCGGCCAGCG 
     5401 GCGAGCGGTA TCAGCTCACT CAAAGGCGGT AATACGGTTA TCCACAGAAT CAGGGGATAA 
     5461 CGCAGGAAAG AACATGTGAG CAAAAGGCCA GCAAAAGGCC AGGAACCGTA AAAAGGCCGC 
     5521 GTTGCTGGCG TTTTTCCATA GGCTCCGCCC CCCTGACGAG CATCACAAAA ATCGACGCTC 
     5581 AAGTCAGAGG TGGCGAAACC CGACAGGACT ATAAAGATAC CAGGCGTTTC CCCCTGGAAG 
     5641 CTCCCTCGTG CGCTCTCCTG TTCCGACCCT GCCGCTTACC GGATACCTGT CCGCCTTTCT 
     5701 CCCTTCGGGA AGCGTGGCGC TTTCTCATAG CTCACGCTGT AGGTATCTCA GTTCGGTGTA 
     5761 GGTCGTTCGC TCCAAGCTGG GCTGTGTGCA CGAACCCCCC GTTCAGCCCG ACCGCTGCGC 
     5821 CTTATCCGGT AACTATCGTC TTGAGTCCAA CCCGGTAAGA CACGACTTAT CGCCACTGGC 
     5881 AGCAGCCACT GGTAACAGGA TTAGCAGAGC GAGGTATGTA GGCGGTGCTA CAGAGTTCTT 
     5941 GAAGTGGTGG CCTAACTACG GCTACACTAG AAGAACAGTA TTTGGTATCT GCGCTCTGCT 
     6001 GAAGCCAGTT ACCTTCGGAA AAAGAGTTGG TAGCTCTTGA TCCGGCAAAC AAACCACCGC 
     6061 TGGTAGCGGT GGTTTTTTTG TTTGCAAGCA GCAGATTACG CGCAGAAAAA AAGGATCTCA 
     6121 AGAAGATCCT TTGATCTTTT CTACGGGGTC TGACGCTCAG TGGAACGAAA ACTCACGTTA 
     6181 AGGGATTTTG GTCATGAGAT TATCAAAAAG GATCTTCACC TAGATCCTTT TAAATTAAAA 
     6241 ATGAAGTTTT AAATCAATCT AAAGTATATA TGAGTAACCT GAGGCTATGG CAGGGCCTGC 
     6301 CGCCCCGACG TTGGCTGCGA GCCCTGGGCC TTCACCCGAA CTTGGGGGGT GGGGTGGGGA 
     6361 AAAGGAAGAA ACGCGGGCGT ATTGGCCCCA ATGGGGTCTC GGTGGGGTAT CGACAGAGTG 
     6421 CCAGCCCTGG GACCGAACCC CGCGTTTATG AACAAACGAC CCAACACCGT GCGTTTTATT 
     6481 CTGTCTTTTT ATTGCCGTCA TAGCGCGGGT TCCTTCCGGT ATTGTCTCCT TCCGTGTTTC 
     6541 AGTTAGCCTC CCCCTAGGGT GGGCGAAGAA CTCCAGCATG AGATCCCCGC GCTGGAGGAT 
     6601 CATCCAGCCG GCGTCCCGGA AAACGATTCC GAAGCCCAAC CTTTCATAGA AGGCGGCGGT 
     6661 GGAATCGAAA TCTCGTGATG GCAGGTTGGG CGTCGCTTGG TCGGTCATTT CGAACCCCAG 
     6721 AGTCCCGCTC AGAAGAACTC GTCAAGAAGG CGATAGAAGG CGATGCGCTG CGAATCGGGA 
     6781 GCGGCGATAC CGTAAAGCAC GAGGAAGCGG TCAGCCCATT CGCCGCCAAG CTCTTCAGCA 
     6841 ATATCACGGG TAGCCAACGC TATGTCCTGA TAGCGATCCG CCACACCCAG CCGGCCACAG 
     6901 TCGATGAATC CAGAAAAGCG GCCATTTTCC ACCATGATAT TCGGCAAGCA GGCATCGCCA 
     6961 TGGGTCACGA CGAGATCCTC GCCGTCGGGC ATGCTCGCCT TGAGCCTGGC GAACAGTTCG 
     7021 GCTGGCGCGA GCCCCTGATG CTCTTCGTCC AGATCATCCT GATCGACAAG ACCGGCTTCC 
     7081 ATCCGAGTAC GTGCTCGCTC GATGCGATGT TTCGCTTGGT GGTCGAATGG GCAGGTAGCC 
     7141 GGATCAAGCG TATGCAGCCG CCGCATTGCA TCAGCCATGA TGGATACTTT CTCGGCAGGA 
     7201 GCAAGGTGAG ATGACAGGAG ATCCTGCCCC GGCACTTCGC CCAATAGCAG CCAGTCCCTT 
     7261 CCCGCTTCAG TGACAACGTC GAGCACAGCT GCGCAAGGAA CGCCCGTCGT GGCCAGCCAC 
     7321 GATAGCCGCG CTGCCTCGTC TTGCAGTTCA TTCAGGGCAC CGGACAGGTC GGTCTTGACA 
     7381 AAAAGAACCG GGCGCCCCTG CGCTGACAGC CGGAACACGG CGGCATCAGA GCAGCCGATT 
     7441 GTCTGTTGTG CCCAGTCATA GCCGAATAGC CTCTCCACCC AAGCGGCCGG AGAACCTGCG 
     7501 TGCAATCCAT CTTGTTCAAT CATGCGAAAC GATCCTCATC CTGTCTCTTG ATCGATCTTT 
     7561 GCAAAAGCCT AGGCCTCCAA AAAAGCCTCC TCACTACTTC TGGAATAGCT CAGAGGCCGA 
     7621 GGCGGCCTCG GCCTCTGCAT AAATAAAAAA AATTAGTCAG CCATGGGGCG GAGAATGGGC 
     7681 GGAACTGGGC GGAGTTAGGG GCGGGATGGG CGGAGTTAGG GGCGGGACTA TGGTTGCTGA 
     7741 CTAATTGAGA TGCATGCTTT GCATACTTCT GCCTGCTGGG GAGCCTGGGG ACTTTCCACA 
     7801 CCTGGTTGCT GACTAATTGA GATGCATGCT TTGCATACTT CTGCCTGCTG GGGAGCCTGG 
     7861 GGACTTTCCA CACCCTAACT GACACACATT CCACAGCTGG TTCTTTCCGC CTCAGGACTC 
     7921 TTCCTTTTTC AATATTATTG AAGCATTTAT CAGGGTTATT GTCTCATGAG CGGATACATA 
     7981 TTTGAATGTA TTTAGAAAAA TAAACAAATA GGGGTTCCGC GCACATTTCC CCGAAAAGTG 
     8041 CCACCTGACG CGCCCTGTAG CGGCGCATTA AGCGCGGCGG GTGTGGTGGT TACGCGCAGC 
     8101 GTGACCGCTA CACTTGCCAG CGCCCTAGCG CCCGCTCCTT TCGCTTTCTT CCCTTCCTTT 
     8161 CTCGCCACGT TCGCCGGCTT TCCCCGTCAA GCTCTAAATC GGGGGCTCCC TTTAGGGTTC 
     8221 CGATTTAGTG CTTTACGGCA CCTCGACCCC AAAAAACTTG ATTAGGGTGA TGGTTCACGT 
     8281 AGTGGGCCAT CGCCCTGATA GACGGTTTTT CGCCCTTTGA CGTTGGAGTC CACGTTCTTT 
     8341 AATAGTGGAC TCTTGTTCCA AACTGGAACA ACACTCAACC CTATCTCGGT CTATTCTTTT 
     8401 GATTTATAAG GGATTTTGCC GATTTCGGCC TATTGGTTAA AAAATGAGCT GATTTAACAA 
     8461 AAATTTAACG CGAATTTT
//