LOCUS       RC213122                7413 bp    DNA     circular  SYN 
DEFINITION  NRG2 (Myc-DDK-tagged)-Human neuregulin 2 (NRG2), transcript variant 2
ACCESSION   NM_013981
VERSION     NM_013981.3
SOURCE      synthetic construct 
FEATURES    Location/Qualifiers 
     misc_feature    201..924 
                     /label="CMV promoter"  
     misc_feature    839..856 
                     /label="Restriction Enzyme"  
     misc_feature    953..971 
                     /label="T7 promoter"  
     misc_feature    3570..3661 
                     /label="Myc-DDK"  
     misc_feature    3691..4276 
                     /label="PolyA signal"  
     misc_feature    3748..3767 
                     /label="Restriction Enzyme"  
     misc_feature    4552..5224 
                     /label="ColE1"  
     misc_feature    5654..6448 
                     /label="Kan/Neo"  
     misc_feature    6483..6785 
                     /label="SV40 ori"  
     misc_feature    6871..7348 
                     /label="f1 ori"  
     misc_feature    1020..1028 
                     /label="Restriction Enzyme"  
     misc_feature    3552..3558 
                     /label="Restriction Enzyme"  
     misc_feature    1020..3552 
                     /label="NRG2"  
ORIGIN                      
        1 AACAAAATAT TAACGCTTAC AATTTCCATT CGCCATTCAG GCTGCGCAAC TGTTGGGAAG 
       61 GGCGATCGGT GCGGGCCTCT TCGCTATTAC GCCAGCTGGC GAAAGGGGGA TGTGCTGCAA 
      121 GGCGATTAAG TTGGGTAACG CCAGGGTTTT CCCAGTCACG ACGTTGTAAA ACGACGGCCA 
      181 GTGCCAAGCT GATCTATACA TTGAATCAAT ATTGGCAATT AGCCATATTA GTCATTGGTT 
      241 ATATAGCATA AATCAATATT GGCTATTGGC CATTGCATAC GTTGTATCTA TATCATAATA 
      301 TGTACATTTA TATTGGCTCA TGTCCAATAT GACCGCCATG TTGACATTGA TTATTGACTA 
      361 GTTATTAATA GTAATCAATT ACGGGGTCAT TAGTTCATAG CCCATATATG GAGTTCCGCG 
      421 TTACATAACT TACGGTAAAT GGCCCGCCTG GCTGACCGCC CAACGACCCC CGCCCATTGA 
      481 CGTCAATAAT GACGTATGTT CCCATAGTAA CGCCAATAGG GACTTTCCAT TGACGTCAAT 
      541 GGGTGGAGTA TTTACGGTAA ACTGCCCACT TGGCAGTACA TCAAGTGTAT CATATGCCAA 
      601 GTCCGCCCCC TATTGACGTC AATGACGGTA AATGGCCCGC CTGGCATTAT GCCCAGTACA 
      661 TGACCTTACG GGACTTTCCT ACTTGGCAGT ACATCTACGT ATTAGTCATC GCTATTACCA 
      721 TGGTGATGCG GTTTTGGCAG TACACCAATG GGCGTGGATA GCGGTTTGAC TCACGGGGAT 
      781 TTCCAAGTCT CCACCCCATT GACGTCAATG GGAGTTTGTT TTGGCACCAA AATCAACGGG 
      841 ACTTTCCAAA ATGTCGTAAT AACCCCGCCC CGTTGACGCA AATGGGCGGT AGGCGTGTAC 
      901 GGTGGGAGGT CTATATAAGC AGAGCTCGTT TAGTGAACCG TCAGAATTTT GTAATACGAC 
      961 TCACTATAGG GCGGCCGGGA ATTCGTCGAC TGGATCCGGT ACCGAGGAGA TCTGCCGCCG 
     1021 CGATCGCCAT GCGGCAGGTT TGCTGCTCAG CGCTGCCGCC GCCGCCACTG GAGAAGGGTC 
     1081 GGTGCAGCAG CTACAGCGAC AGCAGCAGCA GCAGCAGCGA GAGGAGCAGC AGCAGCAGCA 
     1141 GCAGCAGCAG CGAGAGCGGC AGCAGCAGCA GGAGCAGCAG CAACAACAGC AGCATCTCTC 
     1201 GTCCCGCTGC GCCCCCAGAG CCGCGGCCGC AGCAACAGCC GCAGCCCCGC AGCCCCGCAG 
     1261 CCCGGAGAGC CGCCGCCCGT TCGCGAGCCG CAGCCGCCGG CGGCATGAGG CGCGACCCGG 
     1321 CCCCCGGCTT CTCCATGCTG CTCTTCGGTG TGTCGCTCGC CTGCTACTCG CCCAGCCTCA 
     1381 AGTCAGTGCA GGACCAGGCG TACAAGGCAC CCGTGGTGGT GGAGGGCAAG GTACAGGGGC 
     1441 TGGTCCCAGC CGGCGGCTCC AGCTCCAACA GCACCCGAGA GCCGCCCGCC TCGGGTCGGG 
     1501 TGGCGTTGGT AAAGGTGCTG GACAAGTGGC CGCTCCGGAG CGGGGGGCTG CAGCGCGAGC 
     1561 AGGTGATCAG CGTGGGCTCC TGTGTGCCGC TCGAAAGGAA CCAGCGCTAC ATCTTTTTCC 
     1621 TGGAGCCCAC GGAACAGCCC TTAGTCTTTA AGACGGCCTT TGCCCCCCTC GATACCAACG 
     1681 GCAAAAATCT CAAGAAAGAG GTGGGCAAGA TCCTGTGCAC TGACTGCGCC ACCCGGCCCA 
     1741 AGTTGAAGAA GATGAAGAGC CAGACGGGAC AGGTGGGTGA GAAGCAATCG CTGAAGTGTG 
     1801 AGGCAGCAGC CGGTAATCCC CAGCCTTCCT ACCGTTGGTT CAAGGATGGC AAGGAGCTCA 
     1861 ACCGCAGCCG AGACATTCGC ATCAAATATG GCAACGGCAG AAAGAACTCA CGACTACAGT 
     1921 TCAACAAGGT GAAGGTGGAG GACGCTGGGG AGTATGTCTG CGAGGCCGAG AACATCCTGG 
     1981 GGAAGGACAC CGTCCGGGGC CGGCTTTACG TCAACAGCGT GAGCACCACC CTGTCATCCT 
     2041 GGTCGGGGCA CGCCCGGAAG TGCAACGAGA CAGCCAAGTC CTATTGCGTC AATGGAGGCG 
     2101 TCTGCTACTA CATCGAGGGC ATCAACCAGC TCTCCTGCAA GTGTCCTGTG GGATACACCG 
     2161 GGGACAGGTG TCAGCAGTTC GCAATGGTCA ACTTCTCCAA AGCCGAGGAG CTGTACCAGA 
     2221 AGAGGGTCCT GACCATCACG GGCATCTGCG TGGCTCTGCT GGTCGTGGGC ATCGTCTGTG 
     2281 TGGTGGCCTA CTGCAAGACC AAAAAACAGC GGAAGCAGAT GCACAACCAC CTCCGGCAGA 
     2341 ACATGTGCCC GGCCCATCAG AACCGGAGCT TGGCCAATGG GCCCAGCCAC CCCCGGCTGG 
     2401 ACCCAGAGGA GATCCAGATG GCAGATTATA TTTCCAAGAA CGTGCCAGCC ACAGACCATG 
     2461 TCATCAGGAG AGAAACTGAG ACCACCTTCT CTGGGAGCCA CTCCTGTTCT CCTTCTCACC 
     2521 ACTGCTCCAC AGCCACACCC ACCTCCAGCC ACAGACACGA GAGCCACACG TGGAGCCTGG 
     2581 AACGTTCTGA GAGCCTGACT TCTGACTCCC AGTCGGGGAT CATGCTATCA TCAGTGGGTA 
     2641 CCAGCAAATG CAACAGCCCA GCATGTGTGG AGGCCCGGGC AAGGCGGGCA GCAGCCTACA 
     2701 ACCTGGAGGA GCGGCGCAGG GCCACCGCGC CACCCTATCA CGATTCCGTG GACTCCCTTC 
     2761 GCGACTCCCC ACACAGCGAG AGGTACGTGT CGGCCCTGAC CACGCCCGCG CGCCTCTCGC 
     2821 CCGTGGACTT CCACTACTCG CTGGCCACGC AGGTGCCAAC TTTCGAGATC ACGTCCCCCA 
     2881 ACTCGGCGCA CGCCGTGTCG CTGCCGCCGG CGGCGCCCAT CAGTTACCGC CTGGCCGAGC 
     2941 AGCAGCCGTT ACTGCGGCAC CCGGCGCCCC CCGGCCCGGG ACCCGGACCC GGGCCCGGGC 
     3001 CCGGGCCCGG CGCAGACATG CAGCGCAGCT ATGACAGCTA CTATTACCCC GCGGCGGGGC 
     3061 CCGGACCGCG GCGCGGGACC TGCGCGCTCG GCGGCAGCCT GGGCAGCCTG CCTGCCAGCC 
     3121 CCTTCCGCAT CCCCGAGGAC GACGAGTACG AGACCACGCA GGAGTGCGCG CCCCCGCCGC 
     3181 CGCCGCGGCC GCGCGCGCGC GGTGCGTCCC GCAGGACGTC GGCGGGGCCC CGGCGCTGGC 
     3241 GCCGCTCGCG CCTCAACGGG CTGGCGGCGC AGCGCGCACG GGCGGCGAGG GACTCGCTGT 
     3301 CGCTGAGCAG CGGCTCGGGC GGCGGCTCAG CCTCGGCGTC GGACGACGAC GCGGACGACG 
     3361 CGGACGGGGC GCTGGCGGCC GAGAGCACAC CTTTCCTGGG CCTGCGTGGG GCGCACGACG 
     3421 CGCTGCGCTC GGACTCGCCG CCACTGTGCC CGGCGGCCGA CAGCAGGACT TACTACTCAC 
     3481 TGGACAGCCA CAGCACGCGG GCCAGCAGCA GACACAGCCG CGGGCCGCCC CCGCGGGCCA 
     3541 AGCAGGACTC GGCGCCACTC ACGCGTACGC GGCCGCTCGA GCAGAAACTC ATCTCAGAAG 
     3601 AGGATCTGGC AGCAAATGAT ATCCTGGATT ACAAGGATGA CGACGATAAG GTTTAAACGG 
     3661 CCGGCCGCGG TCATAGCTGT TTCCTGAACA GATCCCGGGT GGCATCCCTG TGACCCCTCC 
     3721 CCAGTGCCTC TCCTGGCCCT GGAAGTTGCC ACTCCAGTGC CCACCAGCCT TGTCCTAATA 
     3781 AAATTAAGTT GCATCATTTT GTCTGACTAG GTGTCCTTCT ATAATATTAT GGGGTGGAGG 
     3841 GGGGTGGTAT GGAGCAAGGG GCAAGTTGGG AAGACAACCT GTAGGGCCTG CGGGGTCTAT 
     3901 TGGGAACCAA GCTGGAGTGC AGTGGCACAA TCTTGGCTCA CTGCAATCTC CGCCTCCTGG 
     3961 GTTCAAGCGA TTCTCCTGCC TCAGCCTCCC GAGTTGTTGG GATTCCAGGC ATGCATGACC 
     4021 AGGCTCAGCT AATTTTTGTT TTTTTGGTAG AGACGGGGTT TCACCATATT GGCCAGGCTG 
     4081 GTCTCCAACT CCTAATCTCA GGTGATCTAC CCACCTTGGC CTCCCAAATT GCTGGGATTA 
     4141 CAGGCGTGAA CCACTGCTCC CTTCCCTGTC CTTCTGATTT TAAAATAACT ATACCAGCAG 
     4201 GAGGACGTCC AGACACAGCA TAGGCTACCT GGCCATGCCC AACCGGTGGG ACATTTGAGT 
     4261 TGCTTGCTTG GCACTGTCCT CTCATGCGTT GGGTCCACTC AGTAGATGCC TGTTGAATTG 
     4321 GGTACGCGGC CAGCGGCGAG CGGTATCAGC TCACTCAAAG GCGGTAATAC GGTTATCCAC 
     4381 AGAATCAGGG GATAACGCAG GAAAGAACAT GTGAGCAAAA GGCCAGCAAA AGGCCAGGAA 
     4441 CCGTAAAAAG GCCGCGTTGC TGGCGTTTTT CCATAGGCTC CGCCCCCCTG ACGAGCATCA 
     4501 CAAAAATCGA CGCTCAAGTC AGAGGTGGCG AAACCCGACA GGACTATAAA GATACCAGGC 
     4561 GTTTCCCCCT GGAAGCTCCC TCGTGCGCTC TCCTGTTCCG ACCCTGCCGC TTACCGGATA 
     4621 CCTGTCCGCC TTTCTCCCTT CGGGAAGCGT GGCGCTTTCT CATAGCTCAC GCTGTAGGTA 
     4681 TCTCAGTTCG GTGTAGGTCG TTCGCTCCAA GCTGGGCTGT GTGCACGAAC CCCCCGTTCA 
     4741 GCCCGACCGC TGCGCCTTAT CCGGTAACTA TCGTCTTGAG TCCAACCCGG TAAGACACGA 
     4801 CTTATCGCCA CTGGCAGCAG CCACTGGTAA CAGGATTAGC AGAGCGAGGT ATGTAGGCGG 
     4861 TGCTACAGAG TTCTTGAAGT GGTGGCCTAA CTACGGCTAC ACTAGAAGAA CAGTATTTGG 
     4921 TATCTGCGCT CTGCTGAAGC CAGTTACCTT CGGAAAAAGA GTTGGTAGCT CTTGATCCGG 
     4981 CAAACAAACC ACCGCTGGTA GCGGTGGTTT TTTTGTTTGC AAGCAGCAGA TTACGCGCAG 
     5041 AAAAAAAGGA TCTCAAGAAG ATCCTTTGAT CTTTTCTACG GGGTCTGACG CTCAGTGGAA 
     5101 CGAAAACTCA CGTTAAGGGA TTTTGGTCAT GAGATTATCA AAAAGGATCT TCACCTAGAT 
     5161 CCTTTTAAAT TAAAAATGAA GTTTTAAATC AATCTAAAGT ATATATGAGT AACCTGAGGC 
     5221 TATGGCAGGG CCTGCCGCCC CGACGTTGGC TGCGAGCCCT GGGCCTTCAC CCGAACTTGG 
     5281 GGGGTGGGGT GGGGAAAAGG AAGAAACGCG GGCGTATTGG CCCCAATGGG GTCTCGGTGG 
     5341 GGTATCGACA GAGTGCCAGC CCTGGGACCG AACCCCGCGT TTATGAACAA ACGACCCAAC 
     5401 ACCGTGCGTT TTATTCTGTC TTTTTATTGC CGTCATAGCG CGGGTTCCTT CCGGTATTGT 
     5461 CTCCTTCCGT GTTTCAGTTA GCCTCCCCCT AGGGTGGGCG AAGAACTCCA GCATGAGATC 
     5521 CCCGCGCTGG AGGATCATCC AGCCGGCGTC CCGGAAAACG ATTCCGAAGC CCAACCTTTC 
     5581 ATAGAAGGCG GCGGTGGAAT CGAAATCTCG TGATGGCAGG TTGGGCGTCG CTTGGTCGGT 
     5641 CATTTCGAAC CCCAGAGTCC CGCTCAGAAG AACTCGTCAA GAAGGCGATA GAAGGCGATG 
     5701 CGCTGCGAAT CGGGAGCGGC GATACCGTAA AGCACGAGGA AGCGGTCAGC CCATTCGCCG 
     5761 CCAAGCTCTT CAGCAATATC ACGGGTAGCC AACGCTATGT CCTGATAGCG ATCCGCCACA 
     5821 CCCAGCCGGC CACAGTCGAT GAATCCAGAA AAGCGGCCAT TTTCCACCAT GATATTCGGC 
     5881 AAGCAGGCAT CGCCATGGGT CACGACGAGA TCCTCGCCGT CGGGCATGCT CGCCTTGAGC 
     5941 CTGGCGAACA GTTCGGCTGG CGCGAGCCCC TGATGCTCTT CGTCCAGATC ATCCTGATCG 
     6001 ACAAGACCGG CTTCCATCCG AGTACGTGCT CGCTCGATGC GATGTTTCGC TTGGTGGTCG 
     6061 AATGGGCAGG TAGCCGGATC AAGCGTATGC AGCCGCCGCA TTGCATCAGC CATGATGGAT 
     6121 ACTTTCTCGG CAGGAGCAAG GTGAGATGAC AGGAGATCCT GCCCCGGCAC TTCGCCCAAT 
     6181 AGCAGCCAGT CCCTTCCCGC TTCAGTGACA ACGTCGAGCA CAGCTGCGCA AGGAACGCCC 
     6241 GTCGTGGCCA GCCACGATAG CCGCGCTGCC TCGTCTTGCA GTTCATTCAG GGCACCGGAC 
     6301 AGGTCGGTCT TGACAAAAAG AACCGGGCGC CCCTGCGCTG ACAGCCGGAA CACGGCGGCA 
     6361 TCAGAGCAGC CGATTGTCTG TTGTGCCCAG TCATAGCCGA ATAGCCTCTC CACCCAAGCG 
     6421 GCCGGAGAAC CTGCGTGCAA TCCATCTTGT TCAATCATGC GAAACGATCC TCATCCTGTC 
     6481 TCTTGATCGA TCTTTGCAAA AGCCTAGGCC TCCAAAAAAG CCTCCTCACT ACTTCTGGAA 
     6541 TAGCTCAGAG GCCGAGGCGG CCTCGGCCTC TGCATAAATA AAAAAAATTA GTCAGCCATG 
     6601 GGGCGGAGAA TGGGCGGAAC TGGGCGGAGT TAGGGGCGGG ATGGGCGGAG TTAGGGGCGG 
     6661 GACTATGGTT GCTGACTAAT TGAGATGCAT GCTTTGCATA CTTCTGCCTG CTGGGGAGCC 
     6721 TGGGGACTTT CCACACCTGG TTGCTGACTA ATTGAGATGC ATGCTTTGCA TACTTCTGCC 
     6781 TGCTGGGGAG CCTGGGGACT TTCCACACCC TAACTGACAC ACATTCCACA GCTGGTTCTT 
     6841 TCCGCCTCAG GACTCTTCCT TTTTCAATAT TATTGAAGCA TTTATCAGGG TTATTGTCTC 
     6901 ATGAGCGGAT ACATATTTGA ATGTATTTAG AAAAATAAAC AAATAGGGGT TCCGCGCACA 
     6961 TTTCCCCGAA AAGTGCCACC TGACGCGCCC TGTAGCGGCG CATTAAGCGC GGCGGGTGTG 
     7021 GTGGTTACGC GCAGCGTGAC CGCTACACTT GCCAGCGCCC TAGCGCCCGC TCCTTTCGCT 
     7081 TTCTTCCCTT CCTTTCTCGC CACGTTCGCC GGCTTTCCCC GTCAAGCTCT AAATCGGGGG 
     7141 CTCCCTTTAG GGTTCCGATT TAGTGCTTTA CGGCACCTCG ACCCCAAAAA ACTTGATTAG 
     7201 GGTGATGGTT CACGTAGTGG GCCATCGCCC TGATAGACGG TTTTTCGCCC TTTGACGTTG 
     7261 GAGTCCACGT TCTTTAATAG TGGACTCTTG TTCCAAACTG GAACAACACT CAACCCTATC 
     7321 TCGGTCTATT CTTTTGATTT ATAAGGGATT TTGCCGATTT CGGCCTATTG GTTAAAAAAT 
     7381 GAGCTGATTT AACAAAAATT TAACGCGAAT TTT
//