LOCUS       RC213023                9171 bp    DNA     circular  SYN 
DEFINITION  FGD6 (Myc-DDK-tagged)-Human FYVE, RhoGEF and PH domain containing 6 (FGD6)
ACCESSION   NM_018351
VERSION     NM_018351.2
SOURCE      synthetic construct 
FEATURES    Location/Qualifiers 
     misc_feature    201..924 
                     /label="CMV promoter"  
     misc_feature    839..856 
                     /label="Restriction Enzyme"  
     misc_feature    953..971 
                     /label="T7 promoter"  
     misc_feature    5328..5419 
                     /label="Myc-DDK"  
     misc_feature    5449..6034 
                     /label="PolyA signal"  
     misc_feature    5506..5525 
                     /label="Restriction Enzyme"  
     misc_feature    6310..6982 
                     /label="ColE1"  
     misc_feature    7412..8206 
                     /label="Kan/Neo"  
     misc_feature    8241..8543 
                     /label="SV40 ori"  
     misc_feature    8629..9106 
                     /label="f1 ori"  
     misc_feature    1020..1028 
                     /label="Restriction Enzyme"  
     misc_feature    5310..5316 
                     /label="Restriction Enzyme"  
     misc_feature    1020..5310 
                     /label="FGD6"  
ORIGIN                      
        1 AACAAAATAT TAACGCTTAC AATTTCCATT CGCCATTCAG GCTGCGCAAC TGTTGGGAAG 
       61 GGCGATCGGT GCGGGCCTCT TCGCTATTAC GCCAGCTGGC GAAAGGGGGA TGTGCTGCAA 
      121 GGCGATTAAG TTGGGTAACG CCAGGGTTTT CCCAGTCACG ACGTTGTAAA ACGACGGCCA 
      181 GTGCCAAGCT GATCTATACA TTGAATCAAT ATTGGCAATT AGCCATATTA GTCATTGGTT 
      241 ATATAGCATA AATCAATATT GGCTATTGGC CATTGCATAC GTTGTATCTA TATCATAATA 
      301 TGTACATTTA TATTGGCTCA TGTCCAATAT GACCGCCATG TTGACATTGA TTATTGACTA 
      361 GTTATTAATA GTAATCAATT ACGGGGTCAT TAGTTCATAG CCCATATATG GAGTTCCGCG 
      421 TTACATAACT TACGGTAAAT GGCCCGCCTG GCTGACCGCC CAACGACCCC CGCCCATTGA 
      481 CGTCAATAAT GACGTATGTT CCCATAGTAA CGCCAATAGG GACTTTCCAT TGACGTCAAT 
      541 GGGTGGAGTA TTTACGGTAA ACTGCCCACT TGGCAGTACA TCAAGTGTAT CATATGCCAA 
      601 GTCCGCCCCC TATTGACGTC AATGACGGTA AATGGCCCGC CTGGCATTAT GCCCAGTACA 
      661 TGACCTTACG GGACTTTCCT ACTTGGCAGT ACATCTACGT ATTAGTCATC GCTATTACCA 
      721 TGGTGATGCG GTTTTGGCAG TACACCAATG GGCGTGGATA GCGGTTTGAC TCACGGGGAT 
      781 TTCCAAGTCT CCACCCCATT GACGTCAATG GGAGTTTGTT TTGGCACCAA AATCAACGGG 
      841 ACTTTCCAAA ATGTCGTAAT AACCCCGCCC CGTTGACGCA AATGGGCGGT AGGCGTGTAC 
      901 GGTGGGAGGT CTATATAAGC AGAGCTCGTT TAGTGAACCG TCAGAATTTT GTAATACGAC 
      961 TCACTATAGG GCGGCCGGGA ATTCGTCGAC TGGATCCGGT ACCGAGGAGA TCTGCCGCCG 
     1021 CGATCGCCAT GACTTCTGCA GCCGAGATAA AGAAGCCACC AGTGGCCCCC AAGCCCAAGT 
     1081 TTGTTGTGGC AAATAATAAG CCAGCCCCAC CTCCTATTGC ACCTAAACCC GACATTGTGA 
     1141 TTTCTAGTGT TCCACAGTCG ACAAAGAAAA TGAAACCAGC AATAGCCCCA AAACCAAAAG 
     1201 TCCTGAAGAC CTCACCTGTT CGAGAGATTG GGCAGTCGCC ATCAAGGAAA ATCATGTTGA 
     1261 ACCTGGAAGG GCATAAACAG GAATTAGCTG AAAGCACTGA CAACTTTAAT TGTAAATATG 
     1321 AAGGCAATCA GAGCAATGAT TATATTTCAC CAATGTGTTC CTGCAGTTCT GAGTGTATCC 
     1381 ATAAGCTGGG CCATAGAGAG AATTTGTGTG TAAAGCAGCT TGTTTTAGAG CCCCTGGAAA 
     1441 TGAATGAAAA TTTAGAAAAC AGTAAAATTG ATGAGACTTT GACTATAAAA ACTAGGAGTA 
     1501 AATGTGATTT GTATGGTGAA AAAGCCAAGA ACCAGGGTGG GGTTGTTTTA AAGGCAAGCG 
     1561 TTTTAGAAGA GGAGCTCAAA GATGCCTTAA TACACCAAAT GCCACCTTTT ATTTCTGCAC 
     1621 AGAAGCACAG GCCCACAGAC AGCCCAGAAA TGAATGGTGG CTGTAATTCA AATGGACAAT 
     1681 TCAGAATTGA ATTTGCGGAT TTGTCACCTT CCCCATCCAG CTTTGAAAAA GTTCCTGATC 
     1741 ATCACAGTTG CCACTTACAG CTTCCTAGTG ATGAATGTGA ACATTTTGAA ACTTGCCAGG 
     1801 ATGACAGTGA AAAAAGCAAT AATTGCTTTC AGTCATCTGA ACTAGAGGCT CTGGAAAATG 
     1861 GGAAAAGGAG TACTTTAATA TCTTCAGATG GAGTTAGTAA GAAATCAGAA GTCAAAGACC 
     1921 TTGGTCCCTT AGAAATTCAT TTAGTACCAT ATACCCCAAA ATTTCCAACT CCCAAGCCCA 
     1981 GAAAGACACG AACTGCTCGT CTGTTACGCC AAAAGTGTGT AGATACTCCT AGTGAAAGCA 
     2041 CTGAAGAACC GGGGAATTCA GACAGTAGCT CTTCCTGTCT TACTGAAAAT AGTTTGAAAA 
     2101 TCAATAAAAT CAGTGTTCTG CATCAGAATG TTTTGTGTAA GCAGGAACAG GTGGATAAAA 
     2161 TGAAGCTAGG AAATAAAAGT GAATTGAATA TGGAATCCAA CAGTGATGCA CAGGACTTAG 
     2221 TCAATTCACA GAAAGCCATG TGTAATGAAA CAACTTCCTT TGAAAAAATG GCACCTTCTT 
     2281 TTGATAAAGA CTCTAATTTG AGTTCTGACA GCACAACTGT AGATGGTTCT AGTATGTCGC 
     2341 TTGCTGTGGA CGAAGGGACC GGTTTTATAA GATGTACTGT ATCTATGAGC CTGCCTAAGC 
     2401 AGCTCAAATT AACTTGCAAT GAACATTTGC AATCTGGGAG AAACCTGGGA GTTTCTGCCC 
     2461 CTCAAATGCA AAAGGAATCT GTTATAAAAG AGGAAAATTC TCTACGAATT GTCCCCAAAA 
     2521 AACCTCAAAG ACATAGCTTG CCTGCTACAG GAGTGCTTAA AAAGGCTGCC TCCGAGGAGC 
     2581 TTTTGGAAAA AAGTTCTTAT CCTTCAAGTG AAGAAAAAAG TTCAGAGAAG AGTCTAGAAA 
     2641 GAAATCACCT TCAGCATTTG TGTGCCCAAA ACCGTGGTGT GTCATCCTCC TTTGATATGC 
     2701 CTAAACGGGC TTCAGAAAAG CCAGTGTGGA AGTTACCTCA TCCTATTTTA CCCTTTTCAG 
     2761 GGAACCCAGA ATTCTTAAAG TCTGTCACCG TATCGTCAAA CAGTGAGCCT TCAACAGCCC 
     2821 TAACCAAGCC CAGAGCAAAA TCGTTATCTG CTATGGATGT GGAAAAGTGC ACTAAGCCTT 
     2881 GCAAAGACTC TACAAAGAAA AACTCTTTTA AAAAGTTGCT CAGCATGAAA CTGTCCATCT 
     2941 GTTTCATGAA GAGTGACTTT CAAAAATTTT GGTCCAAGAG TAGCCAACTC GGAGACACCA 
     3001 CCACAGGCCA CCTCTCCAGT GGGGAGCAGA AGGGGATTGA AAGTGATTGG CAAGGCTTGT 
     3061 TGGTAGGAGA GGAGAAGAGA AGTAAACCCA TCAAGGCATA TTCCACAGAA AACTATAGCC 
     3121 TGGAATCTCA AAAGAAGAGG AAGAAGTCTC GGGGCCAGAC CAGTGCAGCT AATGGTCTGA 
     3181 GAGCTGAGTC TTTGGATGAC CAAATGCTCT CCCGGGAGTC ATCATCTCAG GCACCTTACA 
     3241 AGTCTGTTAC AAGCCTCTGT GCACCGGAGT ATGAAAATAT ACGCCATTAT GAGGAAATAC 
     3301 CAGAGTACGA GAACTTGCCA TTTATTATGG CTATACGGAA AACTCAAGAG TTGGAATGGC 
     3361 AGAATTCCAG CAGCATGGAG GACGCTGATG CAAATGTGTA TGAGGTAGAA GAGCCGTATG 
     3421 AAGCTCCAGA TGGCCAGCTG CAGCTTGGAC CCAGACATCA GCATTCCAGT TCAGGAGCAT 
     3481 CCCAGGAGGA ACAGAATGAT CTTGGTCTTG GTGACCTTCC CTCTGATGAG GAGGAAATCA 
     3541 TCAACAGTTC TGATGAAGAT GATGTCAGCT CTGAGTCAAG TAAAGGAGAG CCTGACCCAC 
     3601 TGGAAGATAA ACAGGATGAA GATAATGGAA TGAAAAGTAA AGTTCATCAT ATTGCCAAGG 
     3661 AGATCATGAG CTCAGAGAAA GTGTTTGTGG ATGTGTTAAA ACTTTTGCAT ATTGATTTCC 
     3721 GGGATGCAGT AGCTCATGCT TCCAGGCAAC TTGGGAAACC AGTGATTGAG GACCGGATTC 
     3781 TAAATCAGAT CCTATACTAC TTGCCTCAGC TGTATGAGCT CAACCGGGAT CTCTTGAAGG 
     3841 AACTGGAGGA AAGAATGTTG CACTGGACTG AACAACAAAG AATTGCTGAT ATCTTTGTAA 
     3901 AGAAGGGACC ATATCTAAAA ATGTATTCCA CATACATCAA AGAATTTGAT AAGAATATAG 
     3961 CCTTGCTGGA TGAACAGTGC AAGAAAAATC CAGGTTTTGC TGCTGTTGTT AGAGAATTTG 
     4021 AGATGAGCCC TCGCTGTGCT AATCTGGCCC TCAAGCACTA CCTGCTCAAG CCGGTTCAGA 
     4081 GGATCCCCCA GTACAGGCTG TTGCTGACAG ATTATTTGAA GAATCTCATA GAAGATGCTG 
     4141 GAGATTACAG AGACACTCAA GATGCCCTTG CTGTTGTTAT AGAGGTAGCC AACCACGCCA 
     4201 ATGACACCAT GAAGCAAGGA GACAACTTTC AGAAACTTAT GCAAATTCAG TACAGCTTAA 
     4261 ATGGACACCA TGAAATTGTG CAGCCTGGTC GGGTTTTTCT CAAAGAAGGA ATTCTGATGA 
     4321 AGCTGTCTCG GAAAGTGATG CAACCTCGAA TGTTTTTCCT GTTTAATGAT GCCCTGCTGT 
     4381 ATACAACACC AGTGCAGTCT GGGATGTATA AACTGAACAA CATGCTCTCA CTGGCTGGAA 
     4441 TGAAGGTCAG AAAACCTACC CAAGAAGCCT ATCAGAATGA ATTAAAGATT GAAAGTGTAG 
     4501 AACGTTCCTT CATTCTCTCA GCCAGTTCTG CCACAGAAAG GGATGAATGG CTAGAAGCGA 
     4561 TTTCCAGGGC AATAGAAGAG TATGCCAAGA AAAGAATCAC CTTCTGTCCT AGTAGGAGTC 
     4621 TTGATGAGGC AGACTCAGAA AATAAAGAAG AAGTTAGTCC TCTTGGATCG AAGGCTCCCA 
     4681 TCTGGATTCC TGATACCAGA GCCACAATGT GTATGATCTG CACAAGCGAA TTCACTCTCA 
     4741 CCTGGAGACG ACACCACTGC CGGGCCTGTG GAAAGATTGT ATGCCAAGCT TGTTCGTCTA 
     4801 ATAAGTATGG CTTAGATTAC CTGAAAAATC AACCAGCAAG AGTATGTGAA CATTGTTTCC 
     4861 AAGAACTGCA GAAATTAGAT CACCAGCACT CCCCTAGGAT TGGATCTCCT GGAAATCACA 
     4921 AATCTCCTTC AAGTGCCTTA TCATCAGTCT TACATAGCAT TCCATCAGGG AGGAAACAGA 
     4981 AAAAAATCCC AGCTGCTCTC AAAGAAGTAT CAGCAAACAC AGAGGATTCT TCTATGAGTG 
     5041 GCTACTTGTA CAGATCAAAG GGCAATAAAA AACCCTGGAA ACACTTTTGG TTTGTCATAA 
     5101 AAAATAAAGT ACTATATACA TATGCTGCAA GTGAGGACGT GGCCGCTTTG GAGAGTCAGC 
     5161 CTTTATTAGG ATTCACTGTT ATTCAAGTTA AAGATGAGAA TTCCGAGTCT AAAGTATTTC 
     5221 AGTTACTGCA CAAAAACATG TTATTTTATG TATTCAAAGC AGAGGATGCT CATTCGGCTC 
     5281 AGAAGTGGAT AGAAGCATTT CAGGAAGGCA CAATATTGAC GCGTACGCGG CCGCTCGAGC 
     5341 AGAAACTCAT CTCAGAAGAG GATCTGGCAG CAAATGATAT CCTGGATTAC AAGGATGACG 
     5401 ACGATAAGGT TTAAACGGCC GGCCGCGGTC ATAGCTGTTT CCTGAACAGA TCCCGGGTGG 
     5461 CATCCCTGTG ACCCCTCCCC AGTGCCTCTC CTGGCCCTGG AAGTTGCCAC TCCAGTGCCC 
     5521 ACCAGCCTTG TCCTAATAAA ATTAAGTTGC ATCATTTTGT CTGACTAGGT GTCCTTCTAT 
     5581 AATATTATGG GGTGGAGGGG GGTGGTATGG AGCAAGGGGC AAGTTGGGAA GACAACCTGT 
     5641 AGGGCCTGCG GGGTCTATTG GGAACCAAGC TGGAGTGCAG TGGCACAATC TTGGCTCACT 
     5701 GCAATCTCCG CCTCCTGGGT TCAAGCGATT CTCCTGCCTC AGCCTCCCGA GTTGTTGGGA 
     5761 TTCCAGGCAT GCATGACCAG GCTCAGCTAA TTTTTGTTTT TTTGGTAGAG ACGGGGTTTC 
     5821 ACCATATTGG CCAGGCTGGT CTCCAACTCC TAATCTCAGG TGATCTACCC ACCTTGGCCT 
     5881 CCCAAATTGC TGGGATTACA GGCGTGAACC ACTGCTCCCT TCCCTGTCCT TCTGATTTTA 
     5941 AAATAACTAT ACCAGCAGGA GGACGTCCAG ACACAGCATA GGCTACCTGG CCATGCCCAA 
     6001 CCGGTGGGAC ATTTGAGTTG CTTGCTTGGC ACTGTCCTCT CATGCGTTGG GTCCACTCAG 
     6061 TAGATGCCTG TTGAATTGGG TACGCGGCCA GCGGCGAGCG GTATCAGCTC ACTCAAAGGC 
     6121 GGTAATACGG TTATCCACAG AATCAGGGGA TAACGCAGGA AAGAACATGT GAGCAAAAGG 
     6181 CCAGCAAAAG GCCAGGAACC GTAAAAAGGC CGCGTTGCTG GCGTTTTTCC ATAGGCTCCG 
     6241 CCCCCCTGAC GAGCATCACA AAAATCGACG CTCAAGTCAG AGGTGGCGAA ACCCGACAGG 
     6301 ACTATAAAGA TACCAGGCGT TTCCCCCTGG AAGCTCCCTC GTGCGCTCTC CTGTTCCGAC 
     6361 CCTGCCGCTT ACCGGATACC TGTCCGCCTT TCTCCCTTCG GGAAGCGTGG CGCTTTCTCA 
     6421 TAGCTCACGC TGTAGGTATC TCAGTTCGGT GTAGGTCGTT CGCTCCAAGC TGGGCTGTGT 
     6481 GCACGAACCC CCCGTTCAGC CCGACCGCTG CGCCTTATCC GGTAACTATC GTCTTGAGTC 
     6541 CAACCCGGTA AGACACGACT TATCGCCACT GGCAGCAGCC ACTGGTAACA GGATTAGCAG 
     6601 AGCGAGGTAT GTAGGCGGTG CTACAGAGTT CTTGAAGTGG TGGCCTAACT ACGGCTACAC 
     6661 TAGAAGAACA GTATTTGGTA TCTGCGCTCT GCTGAAGCCA GTTACCTTCG GAAAAAGAGT 
     6721 TGGTAGCTCT TGATCCGGCA AACAAACCAC CGCTGGTAGC GGTGGTTTTT TTGTTTGCAA 
     6781 GCAGCAGATT ACGCGCAGAA AAAAAGGATC TCAAGAAGAT CCTTTGATCT TTTCTACGGG 
     6841 GTCTGACGCT CAGTGGAACG AAAACTCACG TTAAGGGATT TTGGTCATGA GATTATCAAA 
     6901 AAGGATCTTC ACCTAGATCC TTTTAAATTA AAAATGAAGT TTTAAATCAA TCTAAAGTAT 
     6961 ATATGAGTAA CCTGAGGCTA TGGCAGGGCC TGCCGCCCCG ACGTTGGCTG CGAGCCCTGG 
     7021 GCCTTCACCC GAACTTGGGG GGTGGGGTGG GGAAAAGGAA GAAACGCGGG CGTATTGGCC 
     7081 CCAATGGGGT CTCGGTGGGG TATCGACAGA GTGCCAGCCC TGGGACCGAA CCCCGCGTTT 
     7141 ATGAACAAAC GACCCAACAC CGTGCGTTTT ATTCTGTCTT TTTATTGCCG TCATAGCGCG 
     7201 GGTTCCTTCC GGTATTGTCT CCTTCCGTGT TTCAGTTAGC CTCCCCCTAG GGTGGGCGAA 
     7261 GAACTCCAGC ATGAGATCCC CGCGCTGGAG GATCATCCAG CCGGCGTCCC GGAAAACGAT 
     7321 TCCGAAGCCC AACCTTTCAT AGAAGGCGGC GGTGGAATCG AAATCTCGTG ATGGCAGGTT 
     7381 GGGCGTCGCT TGGTCGGTCA TTTCGAACCC CAGAGTCCCG CTCAGAAGAA CTCGTCAAGA 
     7441 AGGCGATAGA AGGCGATGCG CTGCGAATCG GGAGCGGCGA TACCGTAAAG CACGAGGAAG 
     7501 CGGTCAGCCC ATTCGCCGCC AAGCTCTTCA GCAATATCAC GGGTAGCCAA CGCTATGTCC 
     7561 TGATAGCGAT CCGCCACACC CAGCCGGCCA CAGTCGATGA ATCCAGAAAA GCGGCCATTT 
     7621 TCCACCATGA TATTCGGCAA GCAGGCATCG CCATGGGTCA CGACGAGATC CTCGCCGTCG 
     7681 GGCATGCTCG CCTTGAGCCT GGCGAACAGT TCGGCTGGCG CGAGCCCCTG ATGCTCTTCG 
     7741 TCCAGATCAT CCTGATCGAC AAGACCGGCT TCCATCCGAG TACGTGCTCG CTCGATGCGA 
     7801 TGTTTCGCTT GGTGGTCGAA TGGGCAGGTA GCCGGATCAA GCGTATGCAG CCGCCGCATT 
     7861 GCATCAGCCA TGATGGATAC TTTCTCGGCA GGAGCAAGGT GAGATGACAG GAGATCCTGC 
     7921 CCCGGCACTT CGCCCAATAG CAGCCAGTCC CTTCCCGCTT CAGTGACAAC GTCGAGCACA 
     7981 GCTGCGCAAG GAACGCCCGT CGTGGCCAGC CACGATAGCC GCGCTGCCTC GTCTTGCAGT 
     8041 TCATTCAGGG CACCGGACAG GTCGGTCTTG ACAAAAAGAA CCGGGCGCCC CTGCGCTGAC 
     8101 AGCCGGAACA CGGCGGCATC AGAGCAGCCG ATTGTCTGTT GTGCCCAGTC ATAGCCGAAT 
     8161 AGCCTCTCCA CCCAAGCGGC CGGAGAACCT GCGTGCAATC CATCTTGTTC AATCATGCGA 
     8221 AACGATCCTC ATCCTGTCTC TTGATCGATC TTTGCAAAAG CCTAGGCCTC CAAAAAAGCC 
     8281 TCCTCACTAC TTCTGGAATA GCTCAGAGGC CGAGGCGGCC TCGGCCTCTG CATAAATAAA 
     8341 AAAAATTAGT CAGCCATGGG GCGGAGAATG GGCGGAACTG GGCGGAGTTA GGGGCGGGAT 
     8401 GGGCGGAGTT AGGGGCGGGA CTATGGTTGC TGACTAATTG AGATGCATGC TTTGCATACT 
     8461 TCTGCCTGCT GGGGAGCCTG GGGACTTTCC ACACCTGGTT GCTGACTAAT TGAGATGCAT 
     8521 GCTTTGCATA CTTCTGCCTG CTGGGGAGCC TGGGGACTTT CCACACCCTA ACTGACACAC 
     8581 ATTCCACAGC TGGTTCTTTC CGCCTCAGGA CTCTTCCTTT TTCAATATTA TTGAAGCATT 
     8641 TATCAGGGTT ATTGTCTCAT GAGCGGATAC ATATTTGAAT GTATTTAGAA AAATAAACAA 
     8701 ATAGGGGTTC CGCGCACATT TCCCCGAAAA GTGCCACCTG ACGCGCCCTG TAGCGGCGCA 
     8761 TTAAGCGCGG CGGGTGTGGT GGTTACGCGC AGCGTGACCG CTACACTTGC CAGCGCCCTA 
     8821 GCGCCCGCTC CTTTCGCTTT CTTCCCTTCC TTTCTCGCCA CGTTCGCCGG CTTTCCCCGT 
     8881 CAAGCTCTAA ATCGGGGGCT CCCTTTAGGG TTCCGATTTA GTGCTTTACG GCACCTCGAC 
     8941 CCCAAAAAAC TTGATTAGGG TGATGGTTCA CGTAGTGGGC CATCGCCCTG ATAGACGGTT 
     9001 TTTCGCCCTT TGACGTTGGA GTCCACGTTC TTTAATAGTG GACTCTTGTT CCAAACTGGA 
     9061 ACAACACTCA ACCCTATCTC GGTCTATTCT TTTGATTTAT AAGGGATTTT GCCGATTTCG 
     9121 GCCTATTGGT TAAAAAATGA GCTGATTTAA CAAAAATTTA ACGCGAATTT T
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