LOCUS       RC213008                9390 bp    DNA     circular  SYN 
DEFINITION  CPS1 (Myc-DDK-tagged)-Human carbamoyl-phosphate synthase 1, mitochondrial (CPS1), nuclear gene encoding mitochondrial protein, transcript variant 2
ACCESSION   NM_001875
VERSION     NM_001875.2
SOURCE      synthetic construct 
FEATURES    Location/Qualifiers 
     misc_feature    201..924 
                     /label="CMV promoter"  
     misc_feature    839..856 
                     /label="Restriction Enzyme"  
     misc_feature    953..971 
                     /label="T7 promoter"  
     misc_feature    5545..5636 
                     /label="Myc-DDK"  
     misc_feature    5666..6251 
                     /label="PolyA signal"  
     misc_feature    5723..5742 
                     /label="Restriction Enzyme"  
     misc_feature    6527..7199 
                     /label="ColE1"  
     misc_feature    7629..8423 
                     /label="Kan/Neo"  
     misc_feature    8458..8760 
                     /label="SV40 ori"  
     misc_feature    8846..9323 
                     /label="f1 ori"  
     misc_feature    1020..1028 
                     /label="Restriction Enzyme"  
     misc_feature    5520..5527 
                     /label="Restriction Enzyme"  
     misc_feature    1020..5520 
                     /label="CPS1"  
ORIGIN                      
        1 AACAAAATAT TAACGCTTAC AATTTCCATT CGCCATTCAG GCTGCGCAAC TGTTGGGAAG 
       61 GGCGATCGGT GCGGGCCTCT TCGCTATTAC GCCAGCTGGC GAAAGGGGGA TGTGCTGCAA 
      121 GGCGATTAAG TTGGGTAACG CCAGGGTTTT CCCAGTCACG ACGTTGTAAA ACGACGGCCA 
      181 GTGCCAAGCT GATCTATACA TTGAATCAAT ATTGGCAATT AGCCATATTA GTCATTGGTT 
      241 ATATAGCATA AATCAATATT GGCTATTGGC CATTGCATAC GTTGTATCTA TATCATAATA 
      301 TGTACATTTA TATTGGCTCA TGTCCAATAT GACCGCCATG TTGACATTGA TTATTGACTA 
      361 GTTATTAATA GTAATCAATT ACGGGGTCAT TAGTTCATAG CCCATATATG GAGTTCCGCG 
      421 TTACATAACT TACGGTAAAT GGCCCGCCTG GCTGACCGCC CAACGACCCC CGCCCATTGA 
      481 CGTCAATAAT GACGTATGTT CCCATAGTAA CGCCAATAGG GACTTTCCAT TGACGTCAAT 
      541 GGGTGGAGTA TTTACGGTAA ACTGCCCACT TGGCAGTACA TCAAGTGTAT CATATGCCAA 
      601 GTCCGCCCCC TATTGACGTC AATGACGGTA AATGGCCCGC CTGGCATTAT GCCCAGTACA 
      661 TGACCTTACG GGACTTTCCT ACTTGGCAGT ACATCTACGT ATTAGTCATC GCTATTACCA 
      721 TGGTGATGCG GTTTTGGCAG TACACCAATG GGCGTGGATA GCGGTTTGAC TCACGGGGAT 
      781 TTCCAAGTCT CCACCCCATT GACGTCAATG GGAGTTTGTT TTGGCACCAA AATCAACGGG 
      841 ACTTTCCAAA ATGTCGTAAT AACCCCGCCC CGTTGACGCA AATGGGCGGT AGGCGTGTAC 
      901 GGTGGGAGGT CTATATAAGC AGAGCTCGTT TAGTGAACCG TCAGAATTTT GTAATACGAC 
      961 TCACTATAGG GCGGCCGGGA ATTCGTCGAC TGGATCCGGT ACCGAGGAGA TCTGCCGCCG 
     1021 CGATCGCCAT GACGAGGATT TTGACAGCTT TCAAAGTGGT GAGGACACTG AAGACTGGTT 
     1081 TTGGCTTTAC CAATGTGACT GCACACCAAA AATGGAAATT TTCAAGACCT GGCATCAGGC 
     1141 TCCTTTCTGT CAAGGCACAG ACAGCACACA TTGTCCTGGA AGATGGAACT AAGATGAAAG 
     1201 GTTACTCCTT TGGCCATCCA TCCTCTGTTG CTGGTGAAGT GGTTTTTAAT ACTGGCCTGG 
     1261 GAGGGTACCC AGAAGCTATT ACTGACCCTG CCTACAAAGG ACAGATTCTC ACAATGGCCA 
     1321 ACCCTATTAT TGGGAATGGT GGAGCTCCTG ATACTACTGC TCTGGATGAA CTGGGACTTA 
     1381 GCAAATATTT GGAGTCTAAT GGAATCAAGG TTTCAGGTTT GCTGGTGCTG GATTATAGTA 
     1441 AAGACTACAA CCACTGGCTG GCTACCAAGA GTTTAGGGCA ATGGCTACAG GAAGAAAAGG 
     1501 TTCCTGCAAT TTATGGAGTG GACACAAGAA TGCTGACTAA AATAATTCGG GATAAGGGTA 
     1561 CCATGCTTGG GAAGATTGAA TTTGAAGGTC AGCCTGTGGA TTTTGTGGAT CCAAATAAAC 
     1621 AGAATTTGAT TGCTGAGGTT TCAACCAAGG ATGTCAAAGT GTACGGCAAA GGAAACCCCA 
     1681 CAAAAGTGGT AGCTGTAGAC TGTGGGATTA AAAACAATGT AATCCGCCTG CTAGTAAAGC 
     1741 GAGGAGCTGA AGTGCACTTA GTTCCCTGGA ACCATGATTT CACCAAGATG GAGTATGATG 
     1801 GGATTTTGAT CGCGGGAGGA CCGGGGAACC CAGCTCTTGC AGAACCACTA ATTCAGAATG 
     1861 TCAGAAAGAT TTTGGAGAGT GATCGCAAGG AGCCATTGTT TGGAATCAGT ACAGGAAACT 
     1921 TAATAACAGG ATTGGCTGCT GGTGCCAAAA CCTACAAGAT GTCCATGGCC AACAGAGGGC 
     1981 AGAATCAGCC TGTTTTGAAT ATCACAAACA AACAGGCTTT CATTACTGCT CAGAATCATG 
     2041 GCTATGCCTT GGACAACACC CTCCCTGCTG GCTGGAAACC ACTTTTTGTG AATGTCAACG 
     2101 ATCAAACAAA TGAGGGGATT ATGCATGAGA GCAAACCCTT CTTCGCTGTG CAGTTCCACC 
     2161 CAGAGGTCAC CCCGGGGCCA ATAGACACTG AGTACCTGTT TGATTCCTTT TTCTCACTGA 
     2221 TAAAGAAAGG AAAAGCTACC ACCATTACAT CAGTCTTACC GAAGCCAGCA CTAGTTGCAT 
     2281 CTCGGGTTGA GGTTTCCAAA GTCCTTATTC TAGGATCAGG AGGTCTGTCC ATTGGTCAGG 
     2341 CTGGAGAATT TGATTACTCA GGATCTCAAG CTGTAAAAGC CATGAAGGAA GAAAATGTCA 
     2401 AAACTGTTCT GATGAACCCA AACATTGCAT CAGTCCAGAC CAATGAGGTG GGCTTAAAGC 
     2461 AAGCGGATAC TGTCTACTTT CTTCCCATCA CCCCTCAGTT TGTCACAGAG GTCATCAAGG 
     2521 CAGAACAGCC AGATGGGTTA ATTCTGGGCA TGGGTGGCCA GACAGCTCTG AACTGTGGAG 
     2581 TGGAACTATT CAAGAGAGGT GTGCTCAAGG AATATGGTGT GAAAGTCCTG GGAACTTCAG 
     2641 TTGAGTCCAT TATGGCTACG GAAGACAGGC AGCTGTTTTC AGATAAACTA AATGAGATCA 
     2701 ATGAAAAGAT TGCTCCAAGT TTTGCAGTGG AATCGATTGA GGATGCACTG AAGGCAGCAG 
     2761 ACACCATTGG CTACCCAGTG ATGATCCGTT CCGCCTATGC ACTGGGTGGG TTAGGCTCAG 
     2821 GCATCTGTCC CAACAGAGAG ACTTTGATGG ACCTCAGCAC AAAGGCCTTT GCTATGACCA 
     2881 ACCAAATTCT GGTGGAGAAG TCAGTGACAG GTTGGAAAGA AATAGAATAT GAAGTGGTTC 
     2941 GAGATGCTGA TGACAATTGT GTCACTGTCT GTAACATGGA AAATGTTGAT GCCATGGGTG 
     3001 TTCACACAGG TGACTCAGTT GTTGTGGCTC CTGCCCAGAC ACTCTCCAAT GCCGAGTTTC 
     3061 AGATGTTGAG ACGTACTTCA ATCAATGTTG TTCGCCACTT GGGCATTGTG GGTGAATGCA 
     3121 ACATTCAGTT TGCCCTTCAT CCTACCTCAA TGGAATACTG CATCATTGAA GTGAATGCCA 
     3181 GACTGTCCCG AAGCTCTGCT CTGGCCTCAA AAGCCACTGG CTACCCATTG GCATTCATTG 
     3241 CTGCAAAGAT TGCCCTAGGA ATCCCACTTC CAGAAATTAA GAACGTCGTA TCCGGGAAGA 
     3301 CATCAGCCTG TTTTGAACCT AGCCTGGATT ACATGGTCAC CAAGATTCCC CGCTGGGATC 
     3361 TTGACCGTTT TCATGGAACA TCTAGCCGAA TTGGTAGCTC TATGAAAAGT GTAGGAGAGG 
     3421 TCATGGCTAT TGGTCGTACC TTTGAGGAGA GTTTCCAGAA AGCTTTACGG ATGTGCCACC 
     3481 CATCTATAGA AGGTTTCACT CCCCGTCTCC CAATGAACAA AGAATGGCCA TCTAATTTAG 
     3541 ATCTTAGAAA AGAGTTGTCT GAACCAAGCA GCACGCGTAT CTATGCCATT GCCAAGGCCA 
     3601 TTGATGACAA CATGTCCCTT GATGAGATTG AGAAGCTCAC ATACATTGAC AAGTGGTTTT 
     3661 TGTATAAGAT GCGTGATATT TTAAACATGG AAAAGACACT GAAAGGGCTC AACAGTGAGT 
     3721 CCATGACAGA AGAAACCCTG AAAAGGGCAA AGGAGATTGG GTTCTCAGAT AAGCAGATTT 
     3781 CAAAATGCCT TGGGCTCACT GAGGCCCAGA CAAGGGAGCT GAGGTTAAAG AAAAACATCC 
     3841 ACCCTTGGGT TAAACAGATT GATACACTGG CTGCAGAATA CCCATCAGTA ACAAACTATC 
     3901 TCTATGTTAC CTACAATGGT CAGGAGCATG ATGTCAATTT TGATGACCAT GGAATGATGG 
     3961 TGCTAGGCTG TGGTCCATAT CACATTGGCA GCAGTGTGGA ATTTGATTGG TGTGCTGTCT 
     4021 CTAGTATCCG CACACTGCGT CAACTTGGCA AGAAGACGGT GGTGGTGAAT TGCAATCCTG 
     4081 AGACTGTGAG CACAGACTTT GATGAGTGTG ACAAACTGTA CTTTGAAGAG TTGTCCTTGG 
     4141 AGAGAATCCT AGACATCTAC CATCAGGAGG CATGTGGTGG CTGCATCATA TCAGTTGGAG 
     4201 GCCAGATTCC AAACAACCTG GCAGTTCCTC TATACAAGAA TGGTGTCAAG ATCATGGGCA 
     4261 CAAGCCCCCT GCAGATCGAC AGGGCTGAGG ATCGCTCCAT CTTCTCAGCT GTCTTGGATG 
     4321 AGCTGAAGGT GGCTCAGGCA CCTTGGAAAG CTGTTAATAC TTTGAATGAA GCACTGGAAT 
     4381 TTGCAAAGTC TGTGGACTAC CCCTGCTTGT TGAGGCCTTC CTATGTTTTG AGTGGGTCTG 
     4441 CTATGAATGT GGTATTCTCT GAGGATGAGA TGAAAAAATT CCTAGAAGAG GCGACTAGAG 
     4501 TTTCTCAGGA GCACCCAGTG GTCCTGACAA AATTTGTTGA AGGGGCCCGA GAAGTAGAAA 
     4561 TGGACGCTGT TGGCAAAGAT GGAAGGGTTA TCTCTCATGC CATCTCTGAA CATGTTGAAG 
     4621 ATGCAGGTGT CCACTCGGGA GATGCCACTC TGATGCTGCC CACACAAACC ATCAGCCAAG 
     4681 GGGCCATTGA AAAGGTGAAG GATGCTACCC GGAAGATTGC AAAGGCTTTT GCCATCTCTG 
     4741 GTCCATTCAA CGTCCAATTT CTTGTCAAAG GAAATGATGT CTTGGTGATT GAGTGTAACT 
     4801 TGAGAGCTTC TCGATCCTTC CCCTTTGTTT CCAAGACTCT TGGGGTTGAC TTCATTGATG 
     4861 TGGCCACCAA GGTGATGATT GGAGAGAATG TTGATGAGAA ACATCTTCCA ACATTGGACC 
     4921 ATCCCATAAT TCCTGCTGAC TATGTTGCAA TTAAGGCTCC CATGTTTTCC TGGCCCCGGT 
     4981 TGAGGGATGC TGACCCCATT CTGAGATGTG AGATGGCTTC CACTGGAGAG GTGGCTTGCT 
     5041 TTGGTGAAGG TATTCATACA GCCTTCCTAA AGGCAATGCT TTCCACAGGA TTTAAGATAC 
     5101 CCCAGAAAGG CATCCTGATA GGCATCCAGC AATCATTCCG GCCAAGATTC CTTGGTGTGG 
     5161 CTGAACAATT ACACAATGAA GGTTTCAAGC TGTTTGCCAC GGAAGCCACA TCAGACTGGC 
     5221 TCAACGCCAA CAATGTCCCT GCCACCCCAG TGGCATGGCC GTCTCAAGAA GGACAGAATC 
     5281 CCAGCCTCTC TTCCATCAGA AAATTGATTA GAGATGGCAG CATTGACCTA GTGATTAACC 
     5341 TTCCCAACAA CAACACTAAA TTTGTCCATG ATAATTATGT GATTCGGAGG ACAGCTGTTG 
     5401 ATAGTGGAAT CCCTCTCCTC ACTAATTTTC AGGTGACCAA ACTTTTTGCT GAAGCTGTGC 
     5461 AGAAATCTCG CAAGGTGGAC TCCAAGAGTC TTTTCCACTA CAGGCAGTAC AGTGCTGGAA 
     5521 AAGCAGCAAG CGGACCGACG CGTACGCGGC CGCTCGAGCA GAAACTCATC TCAGAAGAGG 
     5581 ATCTGGCAGC AAATGATATC CTGGATTACA AGGATGACGA CGATAAGGTT TAAACGGCCG 
     5641 GCCGCGGTCA TAGCTGTTTC CTGAACAGAT CCCGGGTGGC ATCCCTGTGA CCCCTCCCCA 
     5701 GTGCCTCTCC TGGCCCTGGA AGTTGCCACT CCAGTGCCCA CCAGCCTTGT CCTAATAAAA 
     5761 TTAAGTTGCA TCATTTTGTC TGACTAGGTG TCCTTCTATA ATATTATGGG GTGGAGGGGG 
     5821 GTGGTATGGA GCAAGGGGCA AGTTGGGAAG ACAACCTGTA GGGCCTGCGG GGTCTATTGG 
     5881 GAACCAAGCT GGAGTGCAGT GGCACAATCT TGGCTCACTG CAATCTCCGC CTCCTGGGTT 
     5941 CAAGCGATTC TCCTGCCTCA GCCTCCCGAG TTGTTGGGAT TCCAGGCATG CATGACCAGG 
     6001 CTCAGCTAAT TTTTGTTTTT TTGGTAGAGA CGGGGTTTCA CCATATTGGC CAGGCTGGTC 
     6061 TCCAACTCCT AATCTCAGGT GATCTACCCA CCTTGGCCTC CCAAATTGCT GGGATTACAG 
     6121 GCGTGAACCA CTGCTCCCTT CCCTGTCCTT CTGATTTTAA AATAACTATA CCAGCAGGAG 
     6181 GACGTCCAGA CACAGCATAG GCTACCTGGC CATGCCCAAC CGGTGGGACA TTTGAGTTGC 
     6241 TTGCTTGGCA CTGTCCTCTC ATGCGTTGGG TCCACTCAGT AGATGCCTGT TGAATTGGGT 
     6301 ACGCGGCCAG CGGCGAGCGG TATCAGCTCA CTCAAAGGCG GTAATACGGT TATCCACAGA 
     6361 ATCAGGGGAT AACGCAGGAA AGAACATGTG AGCAAAAGGC CAGCAAAAGG CCAGGAACCG 
     6421 TAAAAAGGCC GCGTTGCTGG CGTTTTTCCA TAGGCTCCGC CCCCCTGACG AGCATCACAA 
     6481 AAATCGACGC TCAAGTCAGA GGTGGCGAAA CCCGACAGGA CTATAAAGAT ACCAGGCGTT 
     6541 TCCCCCTGGA AGCTCCCTCG TGCGCTCTCC TGTTCCGACC CTGCCGCTTA CCGGATACCT 
     6601 GTCCGCCTTT CTCCCTTCGG GAAGCGTGGC GCTTTCTCAT AGCTCACGCT GTAGGTATCT 
     6661 CAGTTCGGTG TAGGTCGTTC GCTCCAAGCT GGGCTGTGTG CACGAACCCC CCGTTCAGCC 
     6721 CGACCGCTGC GCCTTATCCG GTAACTATCG TCTTGAGTCC AACCCGGTAA GACACGACTT 
     6781 ATCGCCACTG GCAGCAGCCA CTGGTAACAG GATTAGCAGA GCGAGGTATG TAGGCGGTGC 
     6841 TACAGAGTTC TTGAAGTGGT GGCCTAACTA CGGCTACACT AGAAGAACAG TATTTGGTAT 
     6901 CTGCGCTCTG CTGAAGCCAG TTACCTTCGG AAAAAGAGTT GGTAGCTCTT GATCCGGCAA 
     6961 ACAAACCACC GCTGGTAGCG GTGGTTTTTT TGTTTGCAAG CAGCAGATTA CGCGCAGAAA 
     7021 AAAAGGATCT CAAGAAGATC CTTTGATCTT TTCTACGGGG TCTGACGCTC AGTGGAACGA 
     7081 AAACTCACGT TAAGGGATTT TGGTCATGAG ATTATCAAAA AGGATCTTCA CCTAGATCCT 
     7141 TTTAAATTAA AAATGAAGTT TTAAATCAAT CTAAAGTATA TATGAGTAAC CTGAGGCTAT 
     7201 GGCAGGGCCT GCCGCCCCGA CGTTGGCTGC GAGCCCTGGG CCTTCACCCG AACTTGGGGG 
     7261 GTGGGGTGGG GAAAAGGAAG AAACGCGGGC GTATTGGCCC CAATGGGGTC TCGGTGGGGT 
     7321 ATCGACAGAG TGCCAGCCCT GGGACCGAAC CCCGCGTTTA TGAACAAACG ACCCAACACC 
     7381 GTGCGTTTTA TTCTGTCTTT TTATTGCCGT CATAGCGCGG GTTCCTTCCG GTATTGTCTC 
     7441 CTTCCGTGTT TCAGTTAGCC TCCCCCTAGG GTGGGCGAAG AACTCCAGCA TGAGATCCCC 
     7501 GCGCTGGAGG ATCATCCAGC CGGCGTCCCG GAAAACGATT CCGAAGCCCA ACCTTTCATA 
     7561 GAAGGCGGCG GTGGAATCGA AATCTCGTGA TGGCAGGTTG GGCGTCGCTT GGTCGGTCAT 
     7621 TTCGAACCCC AGAGTCCCGC TCAGAAGAAC TCGTCAAGAA GGCGATAGAA GGCGATGCGC 
     7681 TGCGAATCGG GAGCGGCGAT ACCGTAAAGC ACGAGGAAGC GGTCAGCCCA TTCGCCGCCA 
     7741 AGCTCTTCAG CAATATCACG GGTAGCCAAC GCTATGTCCT GATAGCGATC CGCCACACCC 
     7801 AGCCGGCCAC AGTCGATGAA TCCAGAAAAG CGGCCATTTT CCACCATGAT ATTCGGCAAG 
     7861 CAGGCATCGC CATGGGTCAC GACGAGATCC TCGCCGTCGG GCATGCTCGC CTTGAGCCTG 
     7921 GCGAACAGTT CGGCTGGCGC GAGCCCCTGA TGCTCTTCGT CCAGATCATC CTGATCGACA 
     7981 AGACCGGCTT CCATCCGAGT ACGTGCTCGC TCGATGCGAT GTTTCGCTTG GTGGTCGAAT 
     8041 GGGCAGGTAG CCGGATCAAG CGTATGCAGC CGCCGCATTG CATCAGCCAT GATGGATACT 
     8101 TTCTCGGCAG GAGCAAGGTG AGATGACAGG AGATCCTGCC CCGGCACTTC GCCCAATAGC 
     8161 AGCCAGTCCC TTCCCGCTTC AGTGACAACG TCGAGCACAG CTGCGCAAGG AACGCCCGTC 
     8221 GTGGCCAGCC ACGATAGCCG CGCTGCCTCG TCTTGCAGTT CATTCAGGGC ACCGGACAGG 
     8281 TCGGTCTTGA CAAAAAGAAC CGGGCGCCCC TGCGCTGACA GCCGGAACAC GGCGGCATCA 
     8341 GAGCAGCCGA TTGTCTGTTG TGCCCAGTCA TAGCCGAATA GCCTCTCCAC CCAAGCGGCC 
     8401 GGAGAACCTG CGTGCAATCC ATCTTGTTCA ATCATGCGAA ACGATCCTCA TCCTGTCTCT 
     8461 TGATCGATCT TTGCAAAAGC CTAGGCCTCC AAAAAAGCCT CCTCACTACT TCTGGAATAG 
     8521 CTCAGAGGCC GAGGCGGCCT CGGCCTCTGC ATAAATAAAA AAAATTAGTC AGCCATGGGG 
     8581 CGGAGAATGG GCGGAACTGG GCGGAGTTAG GGGCGGGATG GGCGGAGTTA GGGGCGGGAC 
     8641 TATGGTTGCT GACTAATTGA GATGCATGCT TTGCATACTT CTGCCTGCTG GGGAGCCTGG 
     8701 GGACTTTCCA CACCTGGTTG CTGACTAATT GAGATGCATG CTTTGCATAC TTCTGCCTGC 
     8761 TGGGGAGCCT GGGGACTTTC CACACCCTAA CTGACACACA TTCCACAGCT GGTTCTTTCC 
     8821 GCCTCAGGAC TCTTCCTTTT TCAATATTAT TGAAGCATTT ATCAGGGTTA TTGTCTCATG 
     8881 AGCGGATACA TATTTGAATG TATTTAGAAA AATAAACAAA TAGGGGTTCC GCGCACATTT 
     8941 CCCCGAAAAG TGCCACCTGA CGCGCCCTGT AGCGGCGCAT TAAGCGCGGC GGGTGTGGTG 
     9001 GTTACGCGCA GCGTGACCGC TACACTTGCC AGCGCCCTAG CGCCCGCTCC TTTCGCTTTC 
     9061 TTCCCTTCCT TTCTCGCCAC GTTCGCCGGC TTTCCCCGTC AAGCTCTAAA TCGGGGGCTC 
     9121 CCTTTAGGGT TCCGATTTAG TGCTTTACGG CACCTCGACC CCAAAAAACT TGATTAGGGT 
     9181 GATGGTTCAC GTAGTGGGCC ATCGCCCTGA TAGACGGTTT TTCGCCCTTT GACGTTGGAG 
     9241 TCCACGTTCT TTAATAGTGG ACTCTTGTTC CAAACTGGAA CAACACTCAA CCCTATCTCG 
     9301 GTCTATTCTT TTGATTTATA AGGGATTTTG CCGATTTCGG CCTATTGGTT AAAAAATGAG 
     9361 CTGATTTAAC AAAAATTTAA CGCGAATTTT 
//