LOCUS       RC213002                6840 bp    DNA     circular  SYN 
DEFINITION  MAN1A1 (Myc-DDK-tagged)-Human mannosidase, alpha, class 1A, member 1 (MAN1A1)
ACCESSION   NM_005907
VERSION     NM_005907.2
SOURCE      synthetic construct 
FEATURES    Location/Qualifiers 
     misc_feature    201..924 
                     /label="CMV promoter"  
     misc_feature    839..856 
                     /label="Restriction Enzyme"  
     misc_feature    953..971 
                     /label="T7 promoter"  
     misc_feature    2997..3088 
                     /label="Myc-DDK"  
     misc_feature    3118..3703 
                     /label="PolyA signal"  
     misc_feature    3175..3194 
                     /label="Restriction Enzyme"  
     misc_feature    3979..4651 
                     /label="ColE1"  
     misc_feature    5081..5875 
                     /label="Kan/Neo"  
     misc_feature    5910..6212 
                     /label="SV40 ori"  
     misc_feature    6298..6775 
                     /label="f1 ori"  
     misc_feature    1020..1028 
                     /label="Restriction Enzyme"  
     misc_feature    2979..2985 
                     /label="Restriction Enzyme"  
     misc_feature    1020..2979 
                     /label="MAN1A1"  
ORIGIN                      
        1 AACAAAATAT TAACGCTTAC AATTTCCATT CGCCATTCAG GCTGCGCAAC TGTTGGGAAG 
       61 GGCGATCGGT GCGGGCCTCT TCGCTATTAC GCCAGCTGGC GAAAGGGGGA TGTGCTGCAA 
      121 GGCGATTAAG TTGGGTAACG CCAGGGTTTT CCCAGTCACG ACGTTGTAAA ACGACGGCCA 
      181 GTGCCAAGCT GATCTATACA TTGAATCAAT ATTGGCAATT AGCCATATTA GTCATTGGTT 
      241 ATATAGCATA AATCAATATT GGCTATTGGC CATTGCATAC GTTGTATCTA TATCATAATA 
      301 TGTACATTTA TATTGGCTCA TGTCCAATAT GACCGCCATG TTGACATTGA TTATTGACTA 
      361 GTTATTAATA GTAATCAATT ACGGGGTCAT TAGTTCATAG CCCATATATG GAGTTCCGCG 
      421 TTACATAACT TACGGTAAAT GGCCCGCCTG GCTGACCGCC CAACGACCCC CGCCCATTGA 
      481 CGTCAATAAT GACGTATGTT CCCATAGTAA CGCCAATAGG GACTTTCCAT TGACGTCAAT 
      541 GGGTGGAGTA TTTACGGTAA ACTGCCCACT TGGCAGTACA TCAAGTGTAT CATATGCCAA 
      601 GTCCGCCCCC TATTGACGTC AATGACGGTA AATGGCCCGC CTGGCATTAT GCCCAGTACA 
      661 TGACCTTACG GGACTTTCCT ACTTGGCAGT ACATCTACGT ATTAGTCATC GCTATTACCA 
      721 TGGTGATGCG GTTTTGGCAG TACACCAATG GGCGTGGATA GCGGTTTGAC TCACGGGGAT 
      781 TTCCAAGTCT CCACCCCATT GACGTCAATG GGAGTTTGTT TTGGCACCAA AATCAACGGG 
      841 ACTTTCCAAA ATGTCGTAAT AACCCCGCCC CGTTGACGCA AATGGGCGGT AGGCGTGTAC 
      901 GGTGGGAGGT CTATATAAGC AGAGCTCGTT TAGTGAACCG TCAGAATTTT GTAATACGAC 
      961 TCACTATAGG GCGGCCGGGA ATTCGTCGAC TGGATCCGGT ACCGAGGAGA TCTGCCGCCG 
     1021 CGATCGCCAT GCCCGTGGGG GGCCTGTTGC CGCTCTTCAG CAGCCCCGCG GGCGGCGTCC 
     1081 TGGGCGGGGG GCTCGGCGGC GGCGGTGGCA GGAAGGGGTC GGGCCCCGCC GCCCTCCGCC 
     1141 TGACGGAGAA GTTCGTGCTG CTGCTGGTAT TCAGCGCCTT CATCACGCTC TGCTTCGGGG 
     1201 CGATCTTCTT CCTGCCAGAC TCCTCCAAGC TGCTCAGCGG GGTCCTGTTC CACTCCAGCC 
     1261 CCGCCTTGCA GCCGGCCGCC GACCACAAGC CCGGGCCCGG GGCGCGCGCC GAGGACGCGG 
     1321 CCGAGGGGCG AGCCCGGCGC CGCGAGGAGG GGGCACCCGG GGACCCGGAG GCCGCCCTGG 
     1381 AGGACAACTT GGCCAGGATC CGCGAAAACC ACGAGCGGGC TCTCAGGGAA GCCAAGGAGA 
     1441 CCCTGCAGAA GCTGCCCGAG GAGATCCAAA GAGACATCCT ACTGGAGAAG AAGAAGGTGG 
     1501 CCCAGGACCA GCTGCGTGAC AAGGCGCCGT TCAGAGGCCT GCCCCCGGTG GACTTCGTGC 
     1561 CCCCAATCGG GGTGGAGAGC CGGGAGCCCG CCGACGCCGC CATCCGCGAG AAAAGGGCAA 
     1621 AGATCAAAGA GATGATGAAA CATGCTTGGA ATAATTATAA AGGTTATGCC TGGGGATTAA 
     1681 ATGAACTCAA ACCTATATCA AAAGGAGGCC ATTCAAGCAG TTTGTTTGGT AACATCAAAG 
     1741 GAGCAACTAT AGTAGATGCC CTGGATACAC TTTTTATTAT GGAAATGAAA CATGAATTTG 
     1801 AAGAAGCAAA ATCATGGGTT GAAGAAAATT TAGATTTTAA TGTGAATGCT GAAATTTCTG 
     1861 TCTTTGAAGT AAATATACGC TTTGTTGGTG GACTACTCTC AGCCTACTAT CTGTCTGGAG 
     1921 AAGAGATTTT TCGAAAGAAA GCAGTGGAAC TTGGGGTAAA ATTGCTACCT GCATTTCATA 
     1981 CTCCCTCTGG AATACCTTGG GCATTGCTGA ATATGAAAAG TGGTATTGGA AGGAACTGGC 
     2041 CCTGGGCCTC TGGAGGCAGC AGTATTCTGG CAGAATTTGG AACCCTGCAT TTGGAGTTTA 
     2101 TGCACTTGAG CCACTTATCA GGAAACCCCA TCTTTGCTGA AAAGGTAATG AATATTCGAA 
     2161 CAGTACTGAA CAAACTGGAA AAACCACAAG GCCTTTATCC TAACTATCTG AATCCCAGTA 
     2221 GTGGACAGTG GGGTCAACAT CATGTATCAG TTGGAGGACT TGGAGACAGC TTCTATGAGT 
     2281 ATTTGCTGAA GGCCTGGTTA ATGTCTGACA AGACAGATCT GGAAGCTAAG AAGATGTATT 
     2341 TTGATGCTGT TCAGGCTATC GAGACTCATT TGATCCGCAA GTCTAGCAGC GGACTAACTT 
     2401 ATATCGCAGA GTGGAAAGGG GGCCTCCTGG AGCACAAGAT GGGCCACCTG ACCTGCTTCG 
     2461 CGGGGGGCAT GTTCGCACTC GGGGCTGATG CAGCTCCCGA AGGCATGGCC CAACACTACC 
     2521 TTGAACTCGG GGCTGAAATT GCCCGTACTT GTCATGAATC ATATAATCGA ACATTTATGA 
     2581 AACTGGGACC AGAAGCTTTC AGATTTGATG GTGGTGTTGA AGCCATCGCT ACAAGACAAA 
     2641 ATGAAAAATA CTACATCTTA CGGCCAGAAG TTATGGAGAC TTACATGTAT ATGTGGAGAC 
     2701 TGACTCATGA TCCAAAGTAC AGGAAATGGG CCTGGGAAGC CGTAGAGGCC TTGGAAAACC 
     2761 ATTGCAGAGT GAATGGAGGC TATTCAGGCC TAAGGGATGT TTACCTTCTT CATGAGAGTT 
     2821 ATGATGATGT GCAGCAGAGT TTCTTCCTGG CAGAGACATT GAAATATTTG TACCTAATAT 
     2881 TTTCTGACGA CGATCTTCTT CCACTGGAGC ATTGGATCTT CAATAGCGAG GCACATCTTC 
     2941 TCCCTATCCT CCCTAAAGAT AAAAAGGAAG TTGAAATCAG AGAGGAAACG CGTACGCGGC 
     3001 CGCTCGAGCA GAAACTCATC TCAGAAGAGG ATCTGGCAGC AAATGATATC CTGGATTACA 
     3061 AGGATGACGA CGATAAGGTT TAAACGGCCG GCCGCGGTCA TAGCTGTTTC CTGAACAGAT 
     3121 CCCGGGTGGC ATCCCTGTGA CCCCTCCCCA GTGCCTCTCC TGGCCCTGGA AGTTGCCACT 
     3181 CCAGTGCCCA CCAGCCTTGT CCTAATAAAA TTAAGTTGCA TCATTTTGTC TGACTAGGTG 
     3241 TCCTTCTATA ATATTATGGG GTGGAGGGGG GTGGTATGGA GCAAGGGGCA AGTTGGGAAG 
     3301 ACAACCTGTA GGGCCTGCGG GGTCTATTGG GAACCAAGCT GGAGTGCAGT GGCACAATCT 
     3361 TGGCTCACTG CAATCTCCGC CTCCTGGGTT CAAGCGATTC TCCTGCCTCA GCCTCCCGAG 
     3421 TTGTTGGGAT TCCAGGCATG CATGACCAGG CTCAGCTAAT TTTTGTTTTT TTGGTAGAGA 
     3481 CGGGGTTTCA CCATATTGGC CAGGCTGGTC TCCAACTCCT AATCTCAGGT GATCTACCCA 
     3541 CCTTGGCCTC CCAAATTGCT GGGATTACAG GCGTGAACCA CTGCTCCCTT CCCTGTCCTT 
     3601 CTGATTTTAA AATAACTATA CCAGCAGGAG GACGTCCAGA CACAGCATAG GCTACCTGGC 
     3661 CATGCCCAAC CGGTGGGACA TTTGAGTTGC TTGCTTGGCA CTGTCCTCTC ATGCGTTGGG 
     3721 TCCACTCAGT AGATGCCTGT TGAATTGGGT ACGCGGCCAG CGGCGAGCGG TATCAGCTCA 
     3781 CTCAAAGGCG GTAATACGGT TATCCACAGA ATCAGGGGAT AACGCAGGAA AGAACATGTG 
     3841 AGCAAAAGGC CAGCAAAAGG CCAGGAACCG TAAAAAGGCC GCGTTGCTGG CGTTTTTCCA 
     3901 TAGGCTCCGC CCCCCTGACG AGCATCACAA AAATCGACGC TCAAGTCAGA GGTGGCGAAA 
     3961 CCCGACAGGA CTATAAAGAT ACCAGGCGTT TCCCCCTGGA AGCTCCCTCG TGCGCTCTCC 
     4021 TGTTCCGACC CTGCCGCTTA CCGGATACCT GTCCGCCTTT CTCCCTTCGG GAAGCGTGGC 
     4081 GCTTTCTCAT AGCTCACGCT GTAGGTATCT CAGTTCGGTG TAGGTCGTTC GCTCCAAGCT 
     4141 GGGCTGTGTG CACGAACCCC CCGTTCAGCC CGACCGCTGC GCCTTATCCG GTAACTATCG 
     4201 TCTTGAGTCC AACCCGGTAA GACACGACTT ATCGCCACTG GCAGCAGCCA CTGGTAACAG 
     4261 GATTAGCAGA GCGAGGTATG TAGGCGGTGC TACAGAGTTC TTGAAGTGGT GGCCTAACTA 
     4321 CGGCTACACT AGAAGAACAG TATTTGGTAT CTGCGCTCTG CTGAAGCCAG TTACCTTCGG 
     4381 AAAAAGAGTT GGTAGCTCTT GATCCGGCAA ACAAACCACC GCTGGTAGCG GTGGTTTTTT 
     4441 TGTTTGCAAG CAGCAGATTA CGCGCAGAAA AAAAGGATCT CAAGAAGATC CTTTGATCTT 
     4501 TTCTACGGGG TCTGACGCTC AGTGGAACGA AAACTCACGT TAAGGGATTT TGGTCATGAG 
     4561 ATTATCAAAA AGGATCTTCA CCTAGATCCT TTTAAATTAA AAATGAAGTT TTAAATCAAT 
     4621 CTAAAGTATA TATGAGTAAC CTGAGGCTAT GGCAGGGCCT GCCGCCCCGA CGTTGGCTGC 
     4681 GAGCCCTGGG CCTTCACCCG AACTTGGGGG GTGGGGTGGG GAAAAGGAAG AAACGCGGGC 
     4741 GTATTGGCCC CAATGGGGTC TCGGTGGGGT ATCGACAGAG TGCCAGCCCT GGGACCGAAC 
     4801 CCCGCGTTTA TGAACAAACG ACCCAACACC GTGCGTTTTA TTCTGTCTTT TTATTGCCGT 
     4861 CATAGCGCGG GTTCCTTCCG GTATTGTCTC CTTCCGTGTT TCAGTTAGCC TCCCCCTAGG 
     4921 GTGGGCGAAG AACTCCAGCA TGAGATCCCC GCGCTGGAGG ATCATCCAGC CGGCGTCCCG 
     4981 GAAAACGATT CCGAAGCCCA ACCTTTCATA GAAGGCGGCG GTGGAATCGA AATCTCGTGA 
     5041 TGGCAGGTTG GGCGTCGCTT GGTCGGTCAT TTCGAACCCC AGAGTCCCGC TCAGAAGAAC 
     5101 TCGTCAAGAA GGCGATAGAA GGCGATGCGC TGCGAATCGG GAGCGGCGAT ACCGTAAAGC 
     5161 ACGAGGAAGC GGTCAGCCCA TTCGCCGCCA AGCTCTTCAG CAATATCACG GGTAGCCAAC 
     5221 GCTATGTCCT GATAGCGATC CGCCACACCC AGCCGGCCAC AGTCGATGAA TCCAGAAAAG 
     5281 CGGCCATTTT CCACCATGAT ATTCGGCAAG CAGGCATCGC CATGGGTCAC GACGAGATCC 
     5341 TCGCCGTCGG GCATGCTCGC CTTGAGCCTG GCGAACAGTT CGGCTGGCGC GAGCCCCTGA 
     5401 TGCTCTTCGT CCAGATCATC CTGATCGACA AGACCGGCTT CCATCCGAGT ACGTGCTCGC 
     5461 TCGATGCGAT GTTTCGCTTG GTGGTCGAAT GGGCAGGTAG CCGGATCAAG CGTATGCAGC 
     5521 CGCCGCATTG CATCAGCCAT GATGGATACT TTCTCGGCAG GAGCAAGGTG AGATGACAGG 
     5581 AGATCCTGCC CCGGCACTTC GCCCAATAGC AGCCAGTCCC TTCCCGCTTC AGTGACAACG 
     5641 TCGAGCACAG CTGCGCAAGG AACGCCCGTC GTGGCCAGCC ACGATAGCCG CGCTGCCTCG 
     5701 TCTTGCAGTT CATTCAGGGC ACCGGACAGG TCGGTCTTGA CAAAAAGAAC CGGGCGCCCC 
     5761 TGCGCTGACA GCCGGAACAC GGCGGCATCA GAGCAGCCGA TTGTCTGTTG TGCCCAGTCA 
     5821 TAGCCGAATA GCCTCTCCAC CCAAGCGGCC GGAGAACCTG CGTGCAATCC ATCTTGTTCA 
     5881 ATCATGCGAA ACGATCCTCA TCCTGTCTCT TGATCGATCT TTGCAAAAGC CTAGGCCTCC 
     5941 AAAAAAGCCT CCTCACTACT TCTGGAATAG CTCAGAGGCC GAGGCGGCCT CGGCCTCTGC 
     6001 ATAAATAAAA AAAATTAGTC AGCCATGGGG CGGAGAATGG GCGGAACTGG GCGGAGTTAG 
     6061 GGGCGGGATG GGCGGAGTTA GGGGCGGGAC TATGGTTGCT GACTAATTGA GATGCATGCT 
     6121 TTGCATACTT CTGCCTGCTG GGGAGCCTGG GGACTTTCCA CACCTGGTTG CTGACTAATT 
     6181 GAGATGCATG CTTTGCATAC TTCTGCCTGC TGGGGAGCCT GGGGACTTTC CACACCCTAA 
     6241 CTGACACACA TTCCACAGCT GGTTCTTTCC GCCTCAGGAC TCTTCCTTTT TCAATATTAT 
     6301 TGAAGCATTT ATCAGGGTTA TTGTCTCATG AGCGGATACA TATTTGAATG TATTTAGAAA 
     6361 AATAAACAAA TAGGGGTTCC GCGCACATTT CCCCGAAAAG TGCCACCTGA CGCGCCCTGT 
     6421 AGCGGCGCAT TAAGCGCGGC GGGTGTGGTG GTTACGCGCA GCGTGACCGC TACACTTGCC 
     6481 AGCGCCCTAG CGCCCGCTCC TTTCGCTTTC TTCCCTTCCT TTCTCGCCAC GTTCGCCGGC 
     6541 TTTCCCCGTC AAGCTCTAAA TCGGGGGCTC CCTTTAGGGT TCCGATTTAG TGCTTTACGG 
     6601 CACCTCGACC CCAAAAAACT TGATTAGGGT GATGGTTCAC GTAGTGGGCC ATCGCCCTGA 
     6661 TAGACGGTTT TTCGCCCTTT GACGTTGGAG TCCACGTTCT TTAATAGTGG ACTCTTGTTC 
     6721 CAAACTGGAA CAACACTCAA CCCTATCTCG GTCTATTCTT TTGATTTATA AGGGATTTTG 
     6781 CCGATTTCGG CCTATTGGTT AAAAAATGAG CTGATTTAAC AAAAATTTAA CGCGAATTTT 
//