LOCUS       RC212944                8229 bp    DNA     circular  SYN 
DEFINITION  PLEKHA5 (Myc-DDK-tagged)-Human pleckstrin homology domain containing, family A member 5 (PLEKHA5), transcript variant 1
ACCESSION   NM_019012
VERSION     NM_019012.2
SOURCE      synthetic construct 
FEATURES    Location/Qualifiers 
     misc_feature    201..924 
                     /label="CMV promoter"  
     misc_feature    839..856 
                     /label="Restriction Enzyme"  
     misc_feature    953..971 
                     /label="T7 promoter"  
     misc_feature    4386..4477 
                     /label="Myc-DDK"  
     misc_feature    4507..5092 
                     /label="PolyA signal"  
     misc_feature    4564..4583 
                     /label="Restriction Enzyme"  
     misc_feature    5368..6040 
                     /label="ColE1"  
     misc_feature    6470..7264 
                     /label="Kan/Neo"  
     misc_feature    7299..7601 
                     /label="SV40 ori"  
     misc_feature    7687..8164 
                     /label="f1 ori"  
     misc_feature    1020..1028 
                     /label="Restriction Enzyme"  
     misc_feature    4368..4374 
                     /label="Restriction Enzyme"  
     misc_feature    1020..4368 
                     /label="PLEKHA5"  
ORIGIN                      
        1 AACAAAATAT TAACGCTTAC AATTTCCATT CGCCATTCAG GCTGCGCAAC TGTTGGGAAG 
       61 GGCGATCGGT GCGGGCCTCT TCGCTATTAC GCCAGCTGGC GAAAGGGGGA TGTGCTGCAA 
      121 GGCGATTAAG TTGGGTAACG CCAGGGTTTT CCCAGTCACG ACGTTGTAAA ACGACGGCCA 
      181 GTGCCAAGCT GATCTATACA TTGAATCAAT ATTGGCAATT AGCCATATTA GTCATTGGTT 
      241 ATATAGCATA AATCAATATT GGCTATTGGC CATTGCATAC GTTGTATCTA TATCATAATA 
      301 TGTACATTTA TATTGGCTCA TGTCCAATAT GACCGCCATG TTGACATTGA TTATTGACTA 
      361 GTTATTAATA GTAATCAATT ACGGGGTCAT TAGTTCATAG CCCATATATG GAGTTCCGCG 
      421 TTACATAACT TACGGTAAAT GGCCCGCCTG GCTGACCGCC CAACGACCCC CGCCCATTGA 
      481 CGTCAATAAT GACGTATGTT CCCATAGTAA CGCCAATAGG GACTTTCCAT TGACGTCAAT 
      541 GGGTGGAGTA TTTACGGTAA ACTGCCCACT TGGCAGTACA TCAAGTGTAT CATATGCCAA 
      601 GTCCGCCCCC TATTGACGTC AATGACGGTA AATGGCCCGC CTGGCATTAT GCCCAGTACA 
      661 TGACCTTACG GGACTTTCCT ACTTGGCAGT ACATCTACGT ATTAGTCATC GCTATTACCA 
      721 TGGTGATGCG GTTTTGGCAG TACACCAATG GGCGTGGATA GCGGTTTGAC TCACGGGGAT 
      781 TTCCAAGTCT CCACCCCATT GACGTCAATG GGAGTTTGTT TTGGCACCAA AATCAACGGG 
      841 ACTTTCCAAA ATGTCGTAAT AACCCCGCCC CGTTGACGCA AATGGGCGGT AGGCGTGTAC 
      901 GGTGGGAGGT CTATATAAGC AGAGCTCGTT TAGTGAACCG TCAGAATTTT GTAATACGAC 
      961 TCACTATAGG GCGGCCGGGA ATTCGTCGAC TGGATCCGGT ACCGAGGAGA TCTGCCGCCG 
     1021 CGATCGCCAT GGCGGCGGAT CTAAACCTGG AGTGGATCTC CCTGCCCCGG TCCTGGACTT 
     1081 ACGGGATCAC CAGGGGCGGC CGAGTCTTCT TCATCAACGA GGAGGCCAAG AGCACCACCT 
     1141 GGCTGCACCC CGTCACCGGC GAGGCGGTGG TCACCGGACA CCGGCGGCAG AGCACAGATT 
     1201 TGCCTACTGG CTGGGAAGAA GCATATACTT TTGAAGGTGC AAGATACTAT ATAAACCATA 
     1261 ATGAAAGGAA AGTGACCTGC AAACATCCAG TCACAGGACA ACCATCACAG GACAATTGTA 
     1321 TTTTTGTAGT GAATGAACAG ACTGTTGCAA CCATGACATC TGAAGAAAAG AAGGAACGGC 
     1381 CAATAAGTAT GATAAATGAA GCTTCTAACT ATAACGTGAC TTCAGATTAT GCAGTGCATC 
     1441 CAATGAGCCC TGTAGGCAGA ACTTCACGAG CTTCAAAAAA AGTTCATAAT TTTGGAAAGA 
     1501 GGTCAAATTC AATTAAAAGG AATCCTAATG CACCGGTTGT CAGACGAGGT TGGCTTTATA 
     1561 AACAGGACAG TACTGGCATG AAATTGTGGA AGAAACGCTG GTTTGTGCTT TCTGACCTTT 
     1621 GCCTCTTTTA TTATAGAGAT GAGAAAGAAG AGGGTATCCT GGGAAGCATA CTGTTACCTA 
     1681 GTTTTCAGAT AGCTTTGCTT ACCTCTGAAG ATCACATTAA TCGCAAATAT GCTTTTAAGG 
     1741 CAGCCCATCC AAACATGCGG ACCTATTATT TCTGCACTGA TACAGGAAAG GAAATGGAGT 
     1801 TGTGGATGAA AGCCATGTTA GATGCTGCCC TAGTACAGAC AGAACCTGTG AAAAGAGTGG 
     1861 ACAAGATTAC ATCTGAAAAT GCACCAACTA AAGAAACCAA TAACATTCCC AACCATAGAG 
     1921 TGCTAATTAA ACCAGAGATC CAAAACAATC AAAAAAACAA GGAAATGAGC AAAATTGAAG 
     1981 AAAAAAAGGC ATTAGAAGCT GAAAAATATG GATTTCAGAA GGATGGTCAA GATAGACCCT 
     2041 TAACAAAAAT TAATAGTGTA AAGCTGAATT CTCTGCCATC TGAATATGAG AGTGGGTCAG 
     2101 CATGCCCTGC TCAGACTGTG CACTACAGAC CAATCAACTT GAGCAGTTCA GAGAACAAAA 
     2161 TAGTCAATGT TAGCCTGGCA GATCTTAGAG GTGGAAATCG CCCCAATACA GGGCCCTTAT 
     2221 ACACAGAGGC CGATCGAGTC ATACAGAGAA CAAATTCAAT GCAGCAGTTG GAACAGTGGA 
     2281 TTAAAATCCA GAAGGGGAGG GGTCATGAAG AAGAAACCAG GGGAGTAATT TCTTACCAAA 
     2341 CATTACCAAG AAATATGCCA AGTCACAGAG CCCAGATTAT GGCCCGCTAC CCTGAAGGTT 
     2401 ATAGAACACT CCCAAGAAAC AGCAAGACAA GGCCTGAAAG TATCTGCAGT GTAACCCCTT 
     2461 CCACTCATGA CAAGACATTA GGACCCGGAG CGGAGGAGAA ACGGAGGTCC ATGAGAGATG 
     2521 ACACAATGTG GCAGCTCTAC GAATGGCAGC AGCGTCAGTT TTATAACAAA CAGAGCACCC 
     2581 TCCCTCGACA CAGTACTTTG AGTAGTCCCA AAACCATGGT AAATATTTCT GACCAGACAA 
     2641 TGCACTCTAT TCCCACATCA CCTTCCCACG GGTCAATAGC TGCTTATCAG GGATACTCCC 
     2701 CTCAACGAAC TTACAGATCG GAAGTGTCTT CACCAATTCA GAGAGGAGAT GTGACAATAG 
     2761 ACCGCAGACA CAGGGCCCAT CACCCTAAGC ATGTCTATGT GCCTGACAGA AGGTCAGTGC 
     2821 CAGCTGGCCT GACTTTACAG TCTGTTAGTC CCCAGAGCCT CCAAGGGAAA ACGCTGTCAC 
     2881 AAGATGAAGG TAGAGGCACA TTATACAAAT ACAGACCTGA AGAAGTAGAT ATTGATGCCA 
     2941 AGTTAAGCCG ATTATGTGAA CAAGATAAAG TGGTGCATGC TCTGGAAGAG AAACTTCAGC 
     3001 AACTCCACAA GGAGAAATAC ACGCTTGAGC AAGCTTTGCT ATCAGCCAGC CAAGAGATAG 
     3061 AAATGCATGC AGATAACCCA GCAGCCATTC AGACAGTGGT GTTACAAAGG GATGATTTAC 
     3121 AAAATGGACT GCTTAGTACG TGTCGAGAAC TTTCTCGAGC CACTGCCGAA TTGGAACGAG 
     3181 CATGGAGAGA ATATGATAAG TTAGAATACG ATGTAACTGT TACCAGGAAC CAGATGCAAG 
     3241 AGCAGCTGGA TCACCTTGGT GAAGTTCAGA CGGAATCAGC AGGAATTCAG CGTGCACAGA 
     3301 TTCAGAAAGA ACTTTGGCGA ATTCAGGATG TCATGGAAGG GCTGAGTAAA CATAAGCAGC 
     3361 AAAGAGGTAC TACAGAAATA GGTATGATAG GATCAAAGCC TTTCTCAACA GTTAAGTACA 
     3421 AAAATGAGGG TCCAGATTAT AGACTCTACA AGAGTGAACC AGAGTTAACA ACAGTGGCAG 
     3481 AAGTTGATGA ATCTAATGGA GAAGAAAAAT CAGAACCTGT TTCAGAGATA GAAACTTCAG 
     3541 TTGTTAAAGG TTCCCACTTT CCTGTTGGAG TAGTCCCTCC AAGAGCAAAA TCACCAACAC 
     3601 CCGAATCTTC GACAATAGCT TCCTATGTAA CCTTGAGGAA AACTAAGAAG ATGATGGATC 
     3661 TAAGAACGGA AAGACCAAGA AGTGCAGTGG AACAGCTCTG TTTGGCTGAA AGTACTCGAC 
     3721 CAAGGATGAC TGTGGAAGAG CAAATGGAAA GAATAAGAAG ACATCAACAA GCGTGCCTGA 
     3781 GGGAGAAGAA AAAAGGGTTA AATGTTATCG GTGCTTCAGA CCAGTCACCC TTACAAAGCC 
     3841 CTTCAAATTT AAGGGATAAT CCATTTAGGA CTACTCAGAC TCGAAGGAGG GATGATAAGG 
     3901 AACTGGACAC TGCCATTAGA GAAAATGATG TAAAGCCAGA CCATGAAACT CCTGCAACAG 
     3961 AAATTGTTCA ACTAAAAGAA ACCGAACCCC AAAATGTGGA CTTCAGCAAA GAGTTAAAAA 
     4021 AAACTGAAAA CATTTCATAT GAAATGCTTT TTGAACCTGA GCCAAATGGA GTAAATTCTG 
     4081 TGGAAATGAT GGATAAAGAA AGAAACAAAG ACAAAATGCC TGAGGATGTT ACATTCAGCC 
     4141 CTCAAGATGA AACACAGACC GCAAATCATA AACCAGAAGA GCATCCTGAA GAAAATACAA 
     4201 AGAACAGTGT TGACGAACAG GAAGAAACTG TTATTTCTTA CGAATCAACT CCTGAGGTTT 
     4261 CTAGAGGAAA TCAAACAATG GCAGTGAAAA GTCTGTCCCC ATCTCCTGAG TCCTCGGCAT 
     4321 CGCCAGTTCC ATCCACTCAG CCGCAGCTCA CAGAAGGATC ACATTTCATG TGTGTGACGC 
     4381 GTACGCGGCC GCTCGAGCAG AAACTCATCT CAGAAGAGGA TCTGGCAGCA AATGATATCC 
     4441 TGGATTACAA GGATGACGAC GATAAGGTTT AAACGGCCGG CCGCGGTCAT AGCTGTTTCC 
     4501 TGAACAGATC CCGGGTGGCA TCCCTGTGAC CCCTCCCCAG TGCCTCTCCT GGCCCTGGAA 
     4561 GTTGCCACTC CAGTGCCCAC CAGCCTTGTC CTAATAAAAT TAAGTTGCAT CATTTTGTCT 
     4621 GACTAGGTGT CCTTCTATAA TATTATGGGG TGGAGGGGGG TGGTATGGAG CAAGGGGCAA 
     4681 GTTGGGAAGA CAACCTGTAG GGCCTGCGGG GTCTATTGGG AACCAAGCTG GAGTGCAGTG 
     4741 GCACAATCTT GGCTCACTGC AATCTCCGCC TCCTGGGTTC AAGCGATTCT CCTGCCTCAG 
     4801 CCTCCCGAGT TGTTGGGATT CCAGGCATGC ATGACCAGGC TCAGCTAATT TTTGTTTTTT 
     4861 TGGTAGAGAC GGGGTTTCAC CATATTGGCC AGGCTGGTCT CCAACTCCTA ATCTCAGGTG 
     4921 ATCTACCCAC CTTGGCCTCC CAAATTGCTG GGATTACAGG CGTGAACCAC TGCTCCCTTC 
     4981 CCTGTCCTTC TGATTTTAAA ATAACTATAC CAGCAGGAGG ACGTCCAGAC ACAGCATAGG 
     5041 CTACCTGGCC ATGCCCAACC GGTGGGACAT TTGAGTTGCT TGCTTGGCAC TGTCCTCTCA 
     5101 TGCGTTGGGT CCACTCAGTA GATGCCTGTT GAATTGGGTA CGCGGCCAGC GGCGAGCGGT 
     5161 ATCAGCTCAC TCAAAGGCGG TAATACGGTT ATCCACAGAA TCAGGGGATA ACGCAGGAAA 
     5221 GAACATGTGA GCAAAAGGCC AGCAAAAGGC CAGGAACCGT AAAAAGGCCG CGTTGCTGGC 
     5281 GTTTTTCCAT AGGCTCCGCC CCCCTGACGA GCATCACAAA AATCGACGCT CAAGTCAGAG 
     5341 GTGGCGAAAC CCGACAGGAC TATAAAGATA CCAGGCGTTT CCCCCTGGAA GCTCCCTCGT 
     5401 GCGCTCTCCT GTTCCGACCC TGCCGCTTAC CGGATACCTG TCCGCCTTTC TCCCTTCGGG 
     5461 AAGCGTGGCG CTTTCTCATA GCTCACGCTG TAGGTATCTC AGTTCGGTGT AGGTCGTTCG 
     5521 CTCCAAGCTG GGCTGTGTGC ACGAACCCCC CGTTCAGCCC GACCGCTGCG CCTTATCCGG 
     5581 TAACTATCGT CTTGAGTCCA ACCCGGTAAG ACACGACTTA TCGCCACTGG CAGCAGCCAC 
     5641 TGGTAACAGG ATTAGCAGAG CGAGGTATGT AGGCGGTGCT ACAGAGTTCT TGAAGTGGTG 
     5701 GCCTAACTAC GGCTACACTA GAAGAACAGT ATTTGGTATC TGCGCTCTGC TGAAGCCAGT 
     5761 TACCTTCGGA AAAAGAGTTG GTAGCTCTTG ATCCGGCAAA CAAACCACCG CTGGTAGCGG 
     5821 TGGTTTTTTT GTTTGCAAGC AGCAGATTAC GCGCAGAAAA AAAGGATCTC AAGAAGATCC 
     5881 TTTGATCTTT TCTACGGGGT CTGACGCTCA GTGGAACGAA AACTCACGTT AAGGGATTTT 
     5941 GGTCATGAGA TTATCAAAAA GGATCTTCAC CTAGATCCTT TTAAATTAAA AATGAAGTTT 
     6001 TAAATCAATC TAAAGTATAT ATGAGTAACC TGAGGCTATG GCAGGGCCTG CCGCCCCGAC 
     6061 GTTGGCTGCG AGCCCTGGGC CTTCACCCGA ACTTGGGGGG TGGGGTGGGG AAAAGGAAGA 
     6121 AACGCGGGCG TATTGGCCCC AATGGGGTCT CGGTGGGGTA TCGACAGAGT GCCAGCCCTG 
     6181 GGACCGAACC CCGCGTTTAT GAACAAACGA CCCAACACCG TGCGTTTTAT TCTGTCTTTT 
     6241 TATTGCCGTC ATAGCGCGGG TTCCTTCCGG TATTGTCTCC TTCCGTGTTT CAGTTAGCCT 
     6301 CCCCCTAGGG TGGGCGAAGA ACTCCAGCAT GAGATCCCCG CGCTGGAGGA TCATCCAGCC 
     6361 GGCGTCCCGG AAAACGATTC CGAAGCCCAA CCTTTCATAG AAGGCGGCGG TGGAATCGAA 
     6421 ATCTCGTGAT GGCAGGTTGG GCGTCGCTTG GTCGGTCATT TCGAACCCCA GAGTCCCGCT 
     6481 CAGAAGAACT CGTCAAGAAG GCGATAGAAG GCGATGCGCT GCGAATCGGG AGCGGCGATA 
     6541 CCGTAAAGCA CGAGGAAGCG GTCAGCCCAT TCGCCGCCAA GCTCTTCAGC AATATCACGG 
     6601 GTAGCCAACG CTATGTCCTG ATAGCGATCC GCCACACCCA GCCGGCCACA GTCGATGAAT 
     6661 CCAGAAAAGC GGCCATTTTC CACCATGATA TTCGGCAAGC AGGCATCGCC ATGGGTCACG 
     6721 ACGAGATCCT CGCCGTCGGG CATGCTCGCC TTGAGCCTGG CGAACAGTTC GGCTGGCGCG 
     6781 AGCCCCTGAT GCTCTTCGTC CAGATCATCC TGATCGACAA GACCGGCTTC CATCCGAGTA 
     6841 CGTGCTCGCT CGATGCGATG TTTCGCTTGG TGGTCGAATG GGCAGGTAGC CGGATCAAGC 
     6901 GTATGCAGCC GCCGCATTGC ATCAGCCATG ATGGATACTT TCTCGGCAGG AGCAAGGTGA 
     6961 GATGACAGGA GATCCTGCCC CGGCACTTCG CCCAATAGCA GCCAGTCCCT TCCCGCTTCA 
     7021 GTGACAACGT CGAGCACAGC TGCGCAAGGA ACGCCCGTCG TGGCCAGCCA CGATAGCCGC 
     7081 GCTGCCTCGT CTTGCAGTTC ATTCAGGGCA CCGGACAGGT CGGTCTTGAC AAAAAGAACC 
     7141 GGGCGCCCCT GCGCTGACAG CCGGAACACG GCGGCATCAG AGCAGCCGAT TGTCTGTTGT 
     7201 GCCCAGTCAT AGCCGAATAG CCTCTCCACC CAAGCGGCCG GAGAACCTGC GTGCAATCCA 
     7261 TCTTGTTCAA TCATGCGAAA CGATCCTCAT CCTGTCTCTT GATCGATCTT TGCAAAAGCC 
     7321 TAGGCCTCCA AAAAAGCCTC CTCACTACTT CTGGAATAGC TCAGAGGCCG AGGCGGCCTC 
     7381 GGCCTCTGCA TAAATAAAAA AAATTAGTCA GCCATGGGGC GGAGAATGGG CGGAACTGGG 
     7441 CGGAGTTAGG GGCGGGATGG GCGGAGTTAG GGGCGGGACT ATGGTTGCTG ACTAATTGAG 
     7501 ATGCATGCTT TGCATACTTC TGCCTGCTGG GGAGCCTGGG GACTTTCCAC ACCTGGTTGC 
     7561 TGACTAATTG AGATGCATGC TTTGCATACT TCTGCCTGCT GGGGAGCCTG GGGACTTTCC 
     7621 ACACCCTAAC TGACACACAT TCCACAGCTG GTTCTTTCCG CCTCAGGACT CTTCCTTTTT 
     7681 CAATATTATT GAAGCATTTA TCAGGGTTAT TGTCTCATGA GCGGATACAT ATTTGAATGT 
     7741 ATTTAGAAAA ATAAACAAAT AGGGGTTCCG CGCACATTTC CCCGAAAAGT GCCACCTGAC 
     7801 GCGCCCTGTA GCGGCGCATT AAGCGCGGCG GGTGTGGTGG TTACGCGCAG CGTGACCGCT 
     7861 ACACTTGCCA GCGCCCTAGC GCCCGCTCCT TTCGCTTTCT TCCCTTCCTT TCTCGCCACG 
     7921 TTCGCCGGCT TTCCCCGTCA AGCTCTAAAT CGGGGGCTCC CTTTAGGGTT CCGATTTAGT 
     7981 GCTTTACGGC ACCTCGACCC CAAAAAACTT GATTAGGGTG ATGGTTCACG TAGTGGGCCA 
     8041 TCGCCCTGAT AGACGGTTTT TCGCCCTTTG ACGTTGGAGT CCACGTTCTT TAATAGTGGA 
     8101 CTCTTGTTCC AAACTGGAAC AACACTCAAC CCTATCTCGG TCTATTCTTT TGATTTATAA 
     8161 GGGATTTTGC CGATTTCGGC CTATTGGTTA AAAAATGAGC TGATTTAACA AAAATTTAAC 
     8221 GCGAATTTT
//