LOCUS       RC212911                8667 bp    DNA     circular  SYN 
DEFINITION  IARS (Myc-DDK-tagged)-Human isoleucyl-tRNA synthetase (IARS), transcript variant short
ACCESSION   NM_002161
VERSION     NM_002161.2
SOURCE      synthetic construct 
FEATURES    Location/Qualifiers 
     misc_feature    201..924 
                     /label="CMV promoter"  
     misc_feature    839..856 
                     /label="Restriction Enzyme"  
     misc_feature    953..971 
                     /label="T7 promoter"  
     misc_feature    4824..4915 
                     /label="Myc-DDK"  
     misc_feature    4945..5530 
                     /label="PolyA signal"  
     misc_feature    5002..5021 
                     /label="Restriction Enzyme"  
     misc_feature    5806..6478 
                     /label="ColE1"  
     misc_feature    6908..7702 
                     /label="Kan/Neo"  
     misc_feature    7737..8039 
                     /label="SV40 ori"  
     misc_feature    8125..8602 
                     /label="f1 ori"  
     misc_feature    1020..1028 
                     /label="Restriction Enzyme"  
     misc_feature    4806..4812 
                     /label="Restriction Enzyme"  
     misc_feature    1020..4806 
                     /label="IARS"  
ORIGIN                      
        1 AACAAAATAT TAACGCTTAC AATTTCCATT CGCCATTCAG GCTGCGCAAC TGTTGGGAAG 
       61 GGCGATCGGT GCGGGCCTCT TCGCTATTAC GCCAGCTGGC GAAAGGGGGA TGTGCTGCAA 
      121 GGCGATTAAG TTGGGTAACG CCAGGGTTTT CCCAGTCACG ACGTTGTAAA ACGACGGCCA 
      181 GTGCCAAGCT GATCTATACA TTGAATCAAT ATTGGCAATT AGCCATATTA GTCATTGGTT 
      241 ATATAGCATA AATCAATATT GGCTATTGGC CATTGCATAC GTTGTATCTA TATCATAATA 
      301 TGTACATTTA TATTGGCTCA TGTCCAATAT GACCGCCATG TTGACATTGA TTATTGACTA 
      361 GTTATTAATA GTAATCAATT ACGGGGTCAT TAGTTCATAG CCCATATATG GAGTTCCGCG 
      421 TTACATAACT TACGGTAAAT GGCCCGCCTG GCTGACCGCC CAACGACCCC CGCCCATTGA 
      481 CGTCAATAAT GACGTATGTT CCCATAGTAA CGCCAATAGG GACTTTCCAT TGACGTCAAT 
      541 GGGTGGAGTA TTTACGGTAA ACTGCCCACT TGGCAGTACA TCAAGTGTAT CATATGCCAA 
      601 GTCCGCCCCC TATTGACGTC AATGACGGTA AATGGCCCGC CTGGCATTAT GCCCAGTACA 
      661 TGACCTTACG GGACTTTCCT ACTTGGCAGT ACATCTACGT ATTAGTCATC GCTATTACCA 
      721 TGGTGATGCG GTTTTGGCAG TACACCAATG GGCGTGGATA GCGGTTTGAC TCACGGGGAT 
      781 TTCCAAGTCT CCACCCCATT GACGTCAATG GGAGTTTGTT TTGGCACCAA AATCAACGGG 
      841 ACTTTCCAAA ATGTCGTAAT AACCCCGCCC CGTTGACGCA AATGGGCGGT AGGCGTGTAC 
      901 GGTGGGAGGT CTATATAAGC AGAGCTCGTT TAGTGAACCG TCAGAATTTT GTAATACGAC 
      961 TCACTATAGG GCGGCCGGGA ATTCGTCGAC TGGATCCGGT ACCGAGGAGA TCTGCCGCCG 
     1021 CGATCGCCAT GCTTCAACAA GTTCCAGAAA ACATAAATTT TCCTGCTGAA GAAGAGAAAA 
     1081 TCTTGGAGTT TTGGACTGAA TTTAATTGTT TTCAGGAATG CTTAAAGCAA TCAAAACATA 
     1141 AACCAAAATT TACCTTCTAT GATGGTCCTC CTTTTGCAAC TGGACTGCCT CACTATGGAC 
     1201 ATATACTTGC GGGTACAATT AAAGATATAG TTACAAGATA TGCTCACCAG AGTGGGTTTC 
     1261 ATGTTGACAG AAGATTTGGA TGGGATTGCC ATGGCTTACC TGTGGAATAT GAAATTGATA 
     1321 AGACACTGGG AATCAGAGGA CCAGAGGATG TGGCCAAAAT GGGGATTACA GAGTATAACA 
     1381 ATCAGTGCCG AGCAATTGTG ATGAGATATT CTGCTGAGTG GAAGTCTACT GTTAGCAGAC 
     1441 TTGGCCGATG GATTGACTTT GACAATGACT ATAAAACTCT GTATCCACAA TTCATGGAAT 
     1501 CAGTCTGGTG GGTCTTCAAA CAACTCTATG ATAAAGGCCT TGTTTATAGA GGTGTGAAAG 
     1561 TCATGCCCTT CTCTACGGCA TGTAACACTC CACTTTCCAA CTTCGAGTCA CACCAGAATT 
     1621 ATAAGGATGT TCAAGATCCT TCAGTATTTG TAACTTTCCC TTTGGAAGAA GATGAAACTG 
     1681 TATCTTTAGT TGCTTGGACA ACCACTCCCT GGACTCTACC TAGTAACCTT GCTGTGTGTG 
     1741 TTAATCCAGA AATGCAATAT GTGAAAATTA AAGATGTTGC CAGAGGACGA TTACTCATTT 
     1801 TAATGGAAGC CAGATTGTCA GCCCTCTATA AATTGGAGAG TGACTATGAG ATCCTTGAAA 
     1861 GATTTCCTGG TGCCTATCTT AAAGGCAAGA AGTACAGGCC CCTGTTTGAC TATTTCCTGA 
     1921 AGTGTAAAGA GAATGGCGCT TTCACTGTGC TTGTTGACAA CTATGTGAAG GAAGAAGAAG 
     1981 GCACAGGGGT TGTCCACCAA GCTCCTTACT TCGGTGCTGA GGACTATCGG GTCTGTATGG 
     2041 ACTTTAACAT TATTCGGAAA GACTCACTCC CTGTTTGCCC TGTGGATGCT TCAGGCTGCT 
     2101 TCACAACGGA GGTGACAGAT TTCGCAGGAC AGTATGTGAA GGATGCTGAC AAAAGTATCA 
     2161 TCAGGACTTT GAAGGAACAA GGCCGACTTC TGGTTGCCAC CACCTTCACT CACAGCTACC 
     2221 CTTTTTGCTG GAGATCAGAC ACTCCTCTAA TTTACAAAGC AGTGCCCAGC TGGTTTGTGC 
     2281 GAGTGGAGAA CATGGTGGAC CAGCTCCTAA GGAACAATGA CCTGTGCTAC TGGGTCCCAG 
     2341 AGTTGGTACG AGAAAAACGA TTTGGAAATT GGCTGAAAGA TGCACGTGAC TGGACAATTT 
     2401 CCAGAAACAG ATACTGGGGC ACCCCCATCC CACTGTGGGT CAGCGATGAC TTTGAGGAGG 
     2461 TGGTATGCAT TGGGTCAGTG GCGGAACTTG AAGAACTGTC AGGAGCAAAG ATCTCAGATC 
     2521 TCCACAGAGA GAGTGTTGAC CACCTGACCA TTCCTTCACG CTGTGGGAAG GGATCCTTGC 
     2581 ACCGCATCTC TGAAGTGTTT GACTGTTGGT TTGAGAGTGG CAGCATGCCC TATGCTCAGG 
     2641 TTCATTACCC GTTTGAAAAC AAGAGGGAGT TTGAGGATGC TTTTCCTGCA GATTTCATTG 
     2701 CCGAGGGCAT CGACCAAACC AGAGGATGGT TTTATACCCT GCTGGTGCTG GCCACGGCCC 
     2761 TCTTTGGACA ACCGCCTTTC AAGAACGTAA TTGTGAATGG GCTTGTCCTG GCAAGTGATG 
     2821 GCCAAAAAAT GAGCAAACGG AAAAAGAATT ATCCAGATCC AGTTTCCATC ATCCAGAAGT 
     2881 ATGGTGCTGA TGCCCTCAGA TTATATCTGA TTAACTCCCC TGTGGTGAGA GCAGAAAACC 
     2941 TCCGCTTTAA AGAAGAGGGT GTGCGGGACG TCCTTAAGGA TGTACTGCTC CCATGGTACA 
     3001 ATGCCTATCG CTTCTTAATC CAGAACGTTC TGAGGCTCCA GAAGGAGGAA GAAATAGAAT 
     3061 TTCTCTACAA TGAGAACACG GTTAGAGAAA GCCCCAACAT TACAGACCGG TGGATCCTGT 
     3121 CCTTCATGCA GTCTCTCATT GGCTTCTTTG AGACTGAAAT GGCAGCTTAT AGGCTTTATA 
     3181 CTGTGGTGCC TCGCCTGGTC AAGTTTGTAG ATATTCTGAC CAATTGGTAT GTTAGAATGA 
     3241 ACCGCAGAAG ATTAAAGGGT GAAAATGGGA TGGAGGATTG TGTCATGGCC CTAGAAACCT 
     3301 TGTTTAGTGT TCTGCTTTCT CTTTGCAGAC TTATAGCTCC CTACACACCT TTTCTCACTG 
     3361 AATTGATGTA CCAGAATCTA AAGGTGCTGA TTGACCCTGT TTCTGTTCAG GACAAGGACA 
     3421 CACTCAGCAT TCACTACCTC ATGCTGCCCC GTGTTCGAGA AGAATTGATT GACAAGAAAA 
     3481 CAGAGAGTGC AGTATCTCAG ATGCAGTCTG TGATTGAACT TGGAAGAGTG ATCAGAGACC 
     3541 GAAAAACTAT TCCCATAAAG TATCCTTTGA AAGAAATTGT GGTTATCCAT CAAGATCCAG 
     3601 AAGCTCTTAA AGATATCAAG TCTTTGGAGA AGTATATCAT TGAGGAACTC AATGTTCGAA 
     3661 AAGTTACACT GTCTACAGAT AAAAACAAGT ATGGCATTCG GCTAAGGGCA GAACCAGATC 
     3721 ACATGGTCCT GGGGAAGCGT CTGAAGGGAG CCTTTAAGGC AGTGATGACG TCCATCAAGC 
     3781 AGTTGAGCAG TGAGGAGCTG GAGCAGTTCC AGAAGACTGG GACCATTGTT GTGGAAGGCC 
     3841 ATGAATTGCA CGATGAAGAC ATCCGCCTCA TGTACACCTT TGATCAGGCC ACAGGTGGGA 
     3901 CTGCGCAATT TGAAGCACAC TCAGATGCTC AGGCTTTGGT CCTCTTAGAT GTCACTCCTG 
     3961 ACCAGTCAAT GGTAGATGAA GGAATGGCTC GGGAAGTCAT CAATCGCATA CAGAAACTTC 
     4021 GCAAAAAGTG CAATCTGGTT CCAACTGATG AAATCACAGT GTACTATAAA GCAAAGTCTG 
     4081 AAGGAACATA TCTGAATAGT GTTATTGAAA GCCACACAGA GTTCATATTT ACCACCATAA 
     4141 AGGCTCCCTT GAAACCATAT CCAGTTTCTC CATCGGATAA AGTCCTTATT CAAGAAAAAA 
     4201 CACAGTTGAA GGGATCTGAA CTGGAAATTA CACTCACCAG AGGATCTTCC CTTCCTGGTC 
     4261 CTGCTTGTGC ATATGTCAAT CTTAACATTT GTGCAAATGG CAGTGAACAA GGTGGAGTAT 
     4321 TGCTCCTGGA AAATCCAAAA GGTGACAATA GGTTGGACCT TTTAAAGCTG AAGAGTGTTG 
     4381 TCACTAGCAT TTTTGGTGTG AAAAATACAG AGCTGGCTGT CTTCCATGAT GAAACAGAAA 
     4441 TACAAAACCA AACTGACTTA CTGAGTCTTA GTGGAAAAAC ACTTTGTGTG ACTGCAGGAT 
     4501 CGGCTCCCTC TCTGATCAAC AGTTCTAGTA CTCTTCTTTG TCAGTATATC AACCTACAGC 
     4561 TCCTGAATGC AAAGCCACAA GAGTGTTTAA TGGGGACAGT GGGCACTCTC CTGCTTGAAA 
     4621 ACCCACTTGG GCAGAATGGA CTCACCCACC AAGGTCTTCT GTATGAAGCA GCCAAGGTGT 
     4681 TTGGCCTTCG GAGCAGGAAG CTAAAGCTGT TTCTGAATGA GACCCAAACG CAGGAAATTA 
     4741 CAGAAGACAT CCCCGTGAAG ACTTTGAATA TGAAGACTGT GTATGTTTCT GTGTTACCAA 
     4801 CAACAGCAGA CTTCACGCGT ACGCGGCCGC TCGAGCAGAA ACTCATCTCA GAAGAGGATC 
     4861 TGGCAGCAAA TGATATCCTG GATTACAAGG ATGACGACGA TAAGGTTTAA ACGGCCGGCC 
     4921 GCGGTCATAG CTGTTTCCTG AACAGATCCC GGGTGGCATC CCTGTGACCC CTCCCCAGTG 
     4981 CCTCTCCTGG CCCTGGAAGT TGCCACTCCA GTGCCCACCA GCCTTGTCCT AATAAAATTA 
     5041 AGTTGCATCA TTTTGTCTGA CTAGGTGTCC TTCTATAATA TTATGGGGTG GAGGGGGGTG 
     5101 GTATGGAGCA AGGGGCAAGT TGGGAAGACA ACCTGTAGGG CCTGCGGGGT CTATTGGGAA 
     5161 CCAAGCTGGA GTGCAGTGGC ACAATCTTGG CTCACTGCAA TCTCCGCCTC CTGGGTTCAA 
     5221 GCGATTCTCC TGCCTCAGCC TCCCGAGTTG TTGGGATTCC AGGCATGCAT GACCAGGCTC 
     5281 AGCTAATTTT TGTTTTTTTG GTAGAGACGG GGTTTCACCA TATTGGCCAG GCTGGTCTCC 
     5341 AACTCCTAAT CTCAGGTGAT CTACCCACCT TGGCCTCCCA AATTGCTGGG ATTACAGGCG 
     5401 TGAACCACTG CTCCCTTCCC TGTCCTTCTG ATTTTAAAAT AACTATACCA GCAGGAGGAC 
     5461 GTCCAGACAC AGCATAGGCT ACCTGGCCAT GCCCAACCGG TGGGACATTT GAGTTGCTTG 
     5521 CTTGGCACTG TCCTCTCATG CGTTGGGTCC ACTCAGTAGA TGCCTGTTGA ATTGGGTACG 
     5581 CGGCCAGCGG CGAGCGGTAT CAGCTCACTC AAAGGCGGTA ATACGGTTAT CCACAGAATC 
     5641 AGGGGATAAC GCAGGAAAGA ACATGTGAGC AAAAGGCCAG CAAAAGGCCA GGAACCGTAA 
     5701 AAAGGCCGCG TTGCTGGCGT TTTTCCATAG GCTCCGCCCC CCTGACGAGC ATCACAAAAA 
     5761 TCGACGCTCA AGTCAGAGGT GGCGAAACCC GACAGGACTA TAAAGATACC AGGCGTTTCC 
     5821 CCCTGGAAGC TCCCTCGTGC GCTCTCCTGT TCCGACCCTG CCGCTTACCG GATACCTGTC 
     5881 CGCCTTTCTC CCTTCGGGAA GCGTGGCGCT TTCTCATAGC TCACGCTGTA GGTATCTCAG 
     5941 TTCGGTGTAG GTCGTTCGCT CCAAGCTGGG CTGTGTGCAC GAACCCCCCG TTCAGCCCGA 
     6001 CCGCTGCGCC TTATCCGGTA ACTATCGTCT TGAGTCCAAC CCGGTAAGAC ACGACTTATC 
     6061 GCCACTGGCA GCAGCCACTG GTAACAGGAT TAGCAGAGCG AGGTATGTAG GCGGTGCTAC 
     6121 AGAGTTCTTG AAGTGGTGGC CTAACTACGG CTACACTAGA AGAACAGTAT TTGGTATCTG 
     6181 CGCTCTGCTG AAGCCAGTTA CCTTCGGAAA AAGAGTTGGT AGCTCTTGAT CCGGCAAACA 
     6241 AACCACCGCT GGTAGCGGTG GTTTTTTTGT TTGCAAGCAG CAGATTACGC GCAGAAAAAA 
     6301 AGGATCTCAA GAAGATCCTT TGATCTTTTC TACGGGGTCT GACGCTCAGT GGAACGAAAA 
     6361 CTCACGTTAA GGGATTTTGG TCATGAGATT ATCAAAAAGG ATCTTCACCT AGATCCTTTT 
     6421 AAATTAAAAA TGAAGTTTTA AATCAATCTA AAGTATATAT GAGTAACCTG AGGCTATGGC 
     6481 AGGGCCTGCC GCCCCGACGT TGGCTGCGAG CCCTGGGCCT TCACCCGAAC TTGGGGGGTG 
     6541 GGGTGGGGAA AAGGAAGAAA CGCGGGCGTA TTGGCCCCAA TGGGGTCTCG GTGGGGTATC 
     6601 GACAGAGTGC CAGCCCTGGG ACCGAACCCC GCGTTTATGA ACAAACGACC CAACACCGTG 
     6661 CGTTTTATTC TGTCTTTTTA TTGCCGTCAT AGCGCGGGTT CCTTCCGGTA TTGTCTCCTT 
     6721 CCGTGTTTCA GTTAGCCTCC CCCTAGGGTG GGCGAAGAAC TCCAGCATGA GATCCCCGCG 
     6781 CTGGAGGATC ATCCAGCCGG CGTCCCGGAA AACGATTCCG AAGCCCAACC TTTCATAGAA 
     6841 GGCGGCGGTG GAATCGAAAT CTCGTGATGG CAGGTTGGGC GTCGCTTGGT CGGTCATTTC 
     6901 GAACCCCAGA GTCCCGCTCA GAAGAACTCG TCAAGAAGGC GATAGAAGGC GATGCGCTGC 
     6961 GAATCGGGAG CGGCGATACC GTAAAGCACG AGGAAGCGGT CAGCCCATTC GCCGCCAAGC 
     7021 TCTTCAGCAA TATCACGGGT AGCCAACGCT ATGTCCTGAT AGCGATCCGC CACACCCAGC 
     7081 CGGCCACAGT CGATGAATCC AGAAAAGCGG CCATTTTCCA CCATGATATT CGGCAAGCAG 
     7141 GCATCGCCAT GGGTCACGAC GAGATCCTCG CCGTCGGGCA TGCTCGCCTT GAGCCTGGCG 
     7201 AACAGTTCGG CTGGCGCGAG CCCCTGATGC TCTTCGTCCA GATCATCCTG ATCGACAAGA 
     7261 CCGGCTTCCA TCCGAGTACG TGCTCGCTCG ATGCGATGTT TCGCTTGGTG GTCGAATGGG 
     7321 CAGGTAGCCG GATCAAGCGT ATGCAGCCGC CGCATTGCAT CAGCCATGAT GGATACTTTC 
     7381 TCGGCAGGAG CAAGGTGAGA TGACAGGAGA TCCTGCCCCG GCACTTCGCC CAATAGCAGC 
     7441 CAGTCCCTTC CCGCTTCAGT GACAACGTCG AGCACAGCTG CGCAAGGAAC GCCCGTCGTG 
     7501 GCCAGCCACG ATAGCCGCGC TGCCTCGTCT TGCAGTTCAT TCAGGGCACC GGACAGGTCG 
     7561 GTCTTGACAA AAAGAACCGG GCGCCCCTGC GCTGACAGCC GGAACACGGC GGCATCAGAG 
     7621 CAGCCGATTG TCTGTTGTGC CCAGTCATAG CCGAATAGCC TCTCCACCCA AGCGGCCGGA 
     7681 GAACCTGCGT GCAATCCATC TTGTTCAATC ATGCGAAACG ATCCTCATCC TGTCTCTTGA 
     7741 TCGATCTTTG CAAAAGCCTA GGCCTCCAAA AAAGCCTCCT CACTACTTCT GGAATAGCTC 
     7801 AGAGGCCGAG GCGGCCTCGG CCTCTGCATA AATAAAAAAA ATTAGTCAGC CATGGGGCGG 
     7861 AGAATGGGCG GAACTGGGCG GAGTTAGGGG CGGGATGGGC GGAGTTAGGG GCGGGACTAT 
     7921 GGTTGCTGAC TAATTGAGAT GCATGCTTTG CATACTTCTG CCTGCTGGGG AGCCTGGGGA 
     7981 CTTTCCACAC CTGGTTGCTG ACTAATTGAG ATGCATGCTT TGCATACTTC TGCCTGCTGG 
     8041 GGAGCCTGGG GACTTTCCAC ACCCTAACTG ACACACATTC CACAGCTGGT TCTTTCCGCC 
     8101 TCAGGACTCT TCCTTTTTCA ATATTATTGA AGCATTTATC AGGGTTATTG TCTCATGAGC 
     8161 GGATACATAT TTGAATGTAT TTAGAAAAAT AAACAAATAG GGGTTCCGCG CACATTTCCC 
     8221 CGAAAAGTGC CACCTGACGC GCCCTGTAGC GGCGCATTAA GCGCGGCGGG TGTGGTGGTT 
     8281 ACGCGCAGCG TGACCGCTAC ACTTGCCAGC GCCCTAGCGC CCGCTCCTTT CGCTTTCTTC 
     8341 CCTTCCTTTC TCGCCACGTT CGCCGGCTTT CCCCGTCAAG CTCTAAATCG GGGGCTCCCT 
     8401 TTAGGGTTCC GATTTAGTGC TTTACGGCAC CTCGACCCCA AAAAACTTGA TTAGGGTGAT 
     8461 GGTTCACGTA GTGGGCCATC GCCCTGATAG ACGGTTTTTC GCCCTTTGAC GTTGGAGTCC 
     8521 ACGTTCTTTA ATAGTGGACT CTTGTTCCAA ACTGGAACAA CACTCAACCC TATCTCGGTC 
     8581 TATTCTTTTG ATTTATAAGG GATTTTGCCG ATTTCGGCCT ATTGGTTAAA AAATGAGCTG 
     8641 ATTTAACAAA AATTTAACGC GAATTTT
//