LOCUS       RC212863                8247 bp    DNA     circular  SYN 
DEFINITION  HDAC5 (Myc-DDK-tagged)-Human histone deacetylase 5 (HDAC5), transcript variant 1
ACCESSION   NM_005474
VERSION     NM_005474.4
SOURCE      synthetic construct 
FEATURES    Location/Qualifiers 
     misc_feature    201..924 
                     /label="CMV promoter"  
     misc_feature    839..856 
                     /label="Restriction Enzyme"  
     misc_feature    953..971 
                     /label="T7 promoter"  
     misc_feature    4404..4495 
                     /label="Myc-DDK"  
     misc_feature    4525..5110 
                     /label="PolyA signal"  
     misc_feature    4582..4601 
                     /label="Restriction Enzyme"  
     misc_feature    5386..6058 
                     /label="ColE1"  
     misc_feature    6488..7282 
                     /label="Kan/Neo"  
     misc_feature    7317..7619 
                     /label="SV40 ori"  
     misc_feature    7705..8182 
                     /label="f1 ori"  
     misc_feature    1020..1028 
                     /label="Restriction Enzyme"  
     misc_feature    4386..4392 
                     /label="Restriction Enzyme"  
     misc_feature    1020..4386 
                     /label="HDAC5"  
ORIGIN                      
        1 AACAAAATAT TAACGCTTAC AATTTCCATT CGCCATTCAG GCTGCGCAAC TGTTGGGAAG 
       61 GGCGATCGGT GCGGGCCTCT TCGCTATTAC GCCAGCTGGC GAAAGGGGGA TGTGCTGCAA 
      121 GGCGATTAAG TTGGGTAACG CCAGGGTTTT CCCAGTCACG ACGTTGTAAA ACGACGGCCA 
      181 GTGCCAAGCT GATCTATACA TTGAATCAAT ATTGGCAATT AGCCATATTA GTCATTGGTT 
      241 ATATAGCATA AATCAATATT GGCTATTGGC CATTGCATAC GTTGTATCTA TATCATAATA 
      301 TGTACATTTA TATTGGCTCA TGTCCAATAT GACCGCCATG TTGACATTGA TTATTGACTA 
      361 GTTATTAATA GTAATCAATT ACGGGGTCAT TAGTTCATAG CCCATATATG GAGTTCCGCG 
      421 TTACATAACT TACGGTAAAT GGCCCGCCTG GCTGACCGCC CAACGACCCC CGCCCATTGA 
      481 CGTCAATAAT GACGTATGTT CCCATAGTAA CGCCAATAGG GACTTTCCAT TGACGTCAAT 
      541 GGGTGGAGTA TTTACGGTAA ACTGCCCACT TGGCAGTACA TCAAGTGTAT CATATGCCAA 
      601 GTCCGCCCCC TATTGACGTC AATGACGGTA AATGGCCCGC CTGGCATTAT GCCCAGTACA 
      661 TGACCTTACG GGACTTTCCT ACTTGGCAGT ACATCTACGT ATTAGTCATC GCTATTACCA 
      721 TGGTGATGCG GTTTTGGCAG TACACCAATG GGCGTGGATA GCGGTTTGAC TCACGGGGAT 
      781 TTCCAAGTCT CCACCCCATT GACGTCAATG GGAGTTTGTT TTGGCACCAA AATCAACGGG 
      841 ACTTTCCAAA ATGTCGTAAT AACCCCGCCC CGTTGACGCA AATGGGCGGT AGGCGTGTAC 
      901 GGTGGGAGGT CTATATAAGC AGAGCTCGTT TAGTGAACCG TCAGAATTTT GTAATACGAC 
      961 TCACTATAGG GCGGCCGGGA ATTCGTCGAC TGGATCCGGT ACCGAGGAGA TCTGCCGCCG 
     1021 CGATCGCCAT GAACTCTCCC AACGAGTCGG ATGGGATGTC AGGTCGGGAA CCATCCTTGG 
     1081 AAATCCTGCC GCGGACTTCT CTGCACAGCA TCCCTGTGAC AGTGGAGGTG AAGCCGGTGC 
     1141 TGCCAAGAGC CATGCCCAGT TCCATGGGGG GTGGGGGTGG AGGCAGCCCC AGCCCTGTGG 
     1201 AGCTACGGGG GGCTCTGGTG GGCTCTGTGG ACCCCACACT GCGGGAGCAG CAACTGCAGC 
     1261 AGGAGCTCCT GGCGCTCAAG CAGCAGCAGC AGCTGCAGAA GCAGCTCCTG TTCGCTGAGT 
     1321 TCCAGAAACA GCATGACCAC CTGACAAGGC AGCATGAGGT CCAGCTGCAG AAGCACCTCA 
     1381 AGCAGCAGCA GGAGATGCTG GCAGCCAAGC AGCAGCAGGA GATGCTGGCA GCCAAGCGGC 
     1441 AGCAGGAGCT GGAGCAGCAG CGGCAGCGGG AGCAGCAGCG GCAGGAAGAG CTGGAGAAGC 
     1501 AGCGGCTGGA GCAGCAGCTG CTCATCCTGC GGAACAAGGA GAAGAGCAAA GAGAGTGCCA 
     1561 TTGCCAGCAC TGAGGTAAAG CTGAGGCTCC AGGAATTCCT CTTGTCGAAG TCAAAGGAGC 
     1621 CCACACCAGG CGGCCTCAAC CATTCCCTCC CACAGCACCC CAAATGCTGG GGAGCCCACC 
     1681 ATGCTTCTTT GGACCAGAGT TCCCCTCCCC AGAGCGGCCC CCCTGGGACG CCTCCCTCCT 
     1741 ACAAACTGCC TTTGCCTGGG CCCTACGACA GTCGAGACGA CTTCCCCCTC CGCAAAACAG 
     1801 CCTCTGAACC CAACTTGAAA GTGCGTTCAA GGCTAAAACA GAAGGTGGCT GAGCGGAGAA 
     1861 GCAGTCCCCT CCTGCGTCGC AAGGATGGGA CTGTTATTAG CACCTTTAAG AAGAGAGCTG 
     1921 TTGAGATCAC AGGTGCCGGG CCTGGGGCGT CGTCCGTGTG TAACAGCGCA CCCGGCTCCG 
     1981 GCCCCAGCTC TCCCAACAGC TCCCACAGCA CCATCGCTGA GAATGGCTTT ACTGGCTCAG 
     2041 TCCCCAACAT CCCCACTGAG ATGCTCCCTC AGCACCGAGC CCTCCCTCTG GACAGCTCCC 
     2101 CCAACCAGTT CAGCCTCTAC ACGTCTCCTT CTCTGCCCAA CATCTCCCTA GGGCTGCAGG 
     2161 CCACGGTCAC TGTCACCAAC TCACACCTCA CTGCCTCCCC GAAGCTGTCG ACACAGCAGG 
     2221 AGGCCGAGAG GCAGGCCCTC CAGTCCCTGC GGCAGGGTGG CACGCTGACC GGCAAGTTCA 
     2281 TGAGCACATC CTCTATTCCT GGCTGCCTGC TGGGCGTGGC ACTGGAGGGC GACGGGAGCC 
     2341 CCCACGGGCA TGCCTCCCTG CTGCAGCATG TGCTGTTGCT GGAGCAGGCC CGGCAGCAGA 
     2401 GCACCCTCAT TGCTGTGCCA CTCCACGGGC AGTCCCCACT AGTGACGGGT GAACGTGTGG 
     2461 CCACCAGCAT GCGGACGGTA GGCAAGCTCC CGCGGCATCG GCCCCTGAGC CGCACTCAGT 
     2521 CCTCACCGCT GCCGCAGAGT CCCCAGGCCC TGCAGCAGCT GGTCATGCAA CAACAGCACC 
     2581 AGCAGTTCCT GGAGAAGCAG AAGCAGCAGC AGCTACAGCT GGGCAAGATC CTCACCAAGA 
     2641 CAGGGGAGCT GCCCAGGCAG CCCACCACCC ACCCTGAGGA GACAGAGGAG GAGCTGACGG 
     2701 AGCAGCAGGA GGTCTTGCTG GGGGAGGGAG CCCTGACCAT GCCCCGGGAG GGCTCCACAG 
     2761 AGAGTGAGAG CACACAGGAA GACCTGGAGG AGGAGGACGA GGAAGACGAT GGGGAGGAGG 
     2821 AGGAGGATTG CATCCAGGTT AAGGACGAGG AGGGCGAGAG TGGTGCTGAG GAGGGGCCCG 
     2881 ACTTGGAGGA GCCTGGTGCT GGATACAAAA AACTGTTCTC AGATGCCCAG CCGCTGCAGC 
     2941 CTTTGCAGGT GTACCAGGCG CCCCTCAGCC TGGCCACTGT GCCCCACCAG GCCCTGGGCC 
     3001 GTACCCAGTC CTCCCCTGCT GCCCCTGGGG GCATGAAGAG CCCCCCAGAC CAGCCCGTCA 
     3061 AGCACCTCTT CACCACAGGT GTGGTCTACG ACACGTTCAT GCTAAAGCAC CAGTGCATGT 
     3121 GCGGGAACAC ACACGTGCAC CCTGAGCATG CTGGCCGGAT CCAGAGCATC TGGTCCCGGC 
     3181 TGCAGGAGAC AGGCCTGCTT AGCAAGTGCG AGCGGATCCG AGGTCGCAAA GCCACGCTAG 
     3241 ATGAGATCCA GACAGTGCAC TCTGAATACC ACACCCTGCT CTATGGGACC AGTCCCCTCA 
     3301 ACCGGCAGAA GCTAGACAGC AAGAAGTTGC TCGGCCCCAT CAGCCAGAAG ATGTATGCTG 
     3361 TGCTGCCTTG TGGGGGCATC GGGGTGGACA GTGACACCGT GTGGAATGAG ATGCACTCCT 
     3421 CCAGTGCTGT GCGCATGGCA GTGGGCTGCC TGCTGGAGCT GGCCTTCAAG GTGGCTGCAG 
     3481 GAGAGCTCAA GAATGGATTT GCCATCATCC GGCCCCCAGG ACACCACGCC GAGGAATCCA 
     3541 CAGCCATGGG ATTCTGCTTC TTCAACTCTG TAGCCATCAC CGCAAAACTC CTACAGCAGA 
     3601 AGTTGAACGT GGGCAAGGTC CTCATCGTGG ACTGGGACAT TCACCATGGC AATGGCACCC 
     3661 AGCAGGCGTT CTACAATGAC CCCTCTGTGC TCTACATCTC TCTGCATCGC TATGACAACG 
     3721 GGAACTTCTT TCCAGGCTCT GGGGCTCCTG AAGAGGTTGG TGGAGGACCA GGCGTGGGGT 
     3781 ACAATGTGAA CGTGGCATGG ACAGGAGGTG TGGACCCCCC CATTGGAGAC GTGGAGTACC 
     3841 TTACAGCCTT CAGGACAGTG GTGATGCCCA TTGCCCACGA GTTCTCACCT GATGTGGTCC 
     3901 TAGTCTCCGC CGGGTTTGAT GCTGTTGAAG GACATCTGTC TCCTCTGGGT GGCTACTCTG 
     3961 TCACCGCCAG ATGTTTTGGC CACTTGACCA GGCAGCTGAT GACCCTGGCA GGGGGCCGGG 
     4021 TGGTGCTGGC CCTGGAGGGA GGCCATGACT TGACCGCCAT CTGTGATGCC TCTGAGGCTT 
     4081 GTGTCTCGGC TCTGCTCAGT GTAGAGCTGC AGCCCTTGGA TGAGGCAGTC TTGCAGCAAA 
     4141 AGCCCAACAT CAACGCAGTG GCCACGCTAG AGAAAGTCAT CGAGATCCAG AGCAAACACT 
     4201 GGAGCTGTGT GCAGAAGTTC GCCGCTGGTC TGGGCCGGTC CCTGCGAGAG GCCCAAGCAG 
     4261 GTGAGACCGA GGAGGCCGAG ACTGTGAGCG CCATGGCCTT GCTGTCGGTG GGGGCCGAGC 
     4321 AGGCCCAGGC TGCGGCAGCC CGGGAACACA GCCCCAGGCC GGCAGAGGAG CCCATGGAGC 
     4381 AGGAGCCTGC CCTGACGCGT ACGCGGCCGC TCGAGCAGAA ACTCATCTCA GAAGAGGATC 
     4441 TGGCAGCAAA TGATATCCTG GATTACAAGG ATGACGACGA TAAGGTTTAA ACGGCCGGCC 
     4501 GCGGTCATAG CTGTTTCCTG AACAGATCCC GGGTGGCATC CCTGTGACCC CTCCCCAGTG 
     4561 CCTCTCCTGG CCCTGGAAGT TGCCACTCCA GTGCCCACCA GCCTTGTCCT AATAAAATTA 
     4621 AGTTGCATCA TTTTGTCTGA CTAGGTGTCC TTCTATAATA TTATGGGGTG GAGGGGGGTG 
     4681 GTATGGAGCA AGGGGCAAGT TGGGAAGACA ACCTGTAGGG CCTGCGGGGT CTATTGGGAA 
     4741 CCAAGCTGGA GTGCAGTGGC ACAATCTTGG CTCACTGCAA TCTCCGCCTC CTGGGTTCAA 
     4801 GCGATTCTCC TGCCTCAGCC TCCCGAGTTG TTGGGATTCC AGGCATGCAT GACCAGGCTC 
     4861 AGCTAATTTT TGTTTTTTTG GTAGAGACGG GGTTTCACCA TATTGGCCAG GCTGGTCTCC 
     4921 AACTCCTAAT CTCAGGTGAT CTACCCACCT TGGCCTCCCA AATTGCTGGG ATTACAGGCG 
     4981 TGAACCACTG CTCCCTTCCC TGTCCTTCTG ATTTTAAAAT AACTATACCA GCAGGAGGAC 
     5041 GTCCAGACAC AGCATAGGCT ACCTGGCCAT GCCCAACCGG TGGGACATTT GAGTTGCTTG 
     5101 CTTGGCACTG TCCTCTCATG CGTTGGGTCC ACTCAGTAGA TGCCTGTTGA ATTGGGTACG 
     5161 CGGCCAGCGG CGAGCGGTAT CAGCTCACTC AAAGGCGGTA ATACGGTTAT CCACAGAATC 
     5221 AGGGGATAAC GCAGGAAAGA ACATGTGAGC AAAAGGCCAG CAAAAGGCCA GGAACCGTAA 
     5281 AAAGGCCGCG TTGCTGGCGT TTTTCCATAG GCTCCGCCCC CCTGACGAGC ATCACAAAAA 
     5341 TCGACGCTCA AGTCAGAGGT GGCGAAACCC GACAGGACTA TAAAGATACC AGGCGTTTCC 
     5401 CCCTGGAAGC TCCCTCGTGC GCTCTCCTGT TCCGACCCTG CCGCTTACCG GATACCTGTC 
     5461 CGCCTTTCTC CCTTCGGGAA GCGTGGCGCT TTCTCATAGC TCACGCTGTA GGTATCTCAG 
     5521 TTCGGTGTAG GTCGTTCGCT CCAAGCTGGG CTGTGTGCAC GAACCCCCCG TTCAGCCCGA 
     5581 CCGCTGCGCC TTATCCGGTA ACTATCGTCT TGAGTCCAAC CCGGTAAGAC ACGACTTATC 
     5641 GCCACTGGCA GCAGCCACTG GTAACAGGAT TAGCAGAGCG AGGTATGTAG GCGGTGCTAC 
     5701 AGAGTTCTTG AAGTGGTGGC CTAACTACGG CTACACTAGA AGAACAGTAT TTGGTATCTG 
     5761 CGCTCTGCTG AAGCCAGTTA CCTTCGGAAA AAGAGTTGGT AGCTCTTGAT CCGGCAAACA 
     5821 AACCACCGCT GGTAGCGGTG GTTTTTTTGT TTGCAAGCAG CAGATTACGC GCAGAAAAAA 
     5881 AGGATCTCAA GAAGATCCTT TGATCTTTTC TACGGGGTCT GACGCTCAGT GGAACGAAAA 
     5941 CTCACGTTAA GGGATTTTGG TCATGAGATT ATCAAAAAGG ATCTTCACCT AGATCCTTTT 
     6001 AAATTAAAAA TGAAGTTTTA AATCAATCTA AAGTATATAT GAGTAACCTG AGGCTATGGC 
     6061 AGGGCCTGCC GCCCCGACGT TGGCTGCGAG CCCTGGGCCT TCACCCGAAC TTGGGGGGTG 
     6121 GGGTGGGGAA AAGGAAGAAA CGCGGGCGTA TTGGCCCCAA TGGGGTCTCG GTGGGGTATC 
     6181 GACAGAGTGC CAGCCCTGGG ACCGAACCCC GCGTTTATGA ACAAACGACC CAACACCGTG 
     6241 CGTTTTATTC TGTCTTTTTA TTGCCGTCAT AGCGCGGGTT CCTTCCGGTA TTGTCTCCTT 
     6301 CCGTGTTTCA GTTAGCCTCC CCCTAGGGTG GGCGAAGAAC TCCAGCATGA GATCCCCGCG 
     6361 CTGGAGGATC ATCCAGCCGG CGTCCCGGAA AACGATTCCG AAGCCCAACC TTTCATAGAA 
     6421 GGCGGCGGTG GAATCGAAAT CTCGTGATGG CAGGTTGGGC GTCGCTTGGT CGGTCATTTC 
     6481 GAACCCCAGA GTCCCGCTCA GAAGAACTCG TCAAGAAGGC GATAGAAGGC GATGCGCTGC 
     6541 GAATCGGGAG CGGCGATACC GTAAAGCACG AGGAAGCGGT CAGCCCATTC GCCGCCAAGC 
     6601 TCTTCAGCAA TATCACGGGT AGCCAACGCT ATGTCCTGAT AGCGATCCGC CACACCCAGC 
     6661 CGGCCACAGT CGATGAATCC AGAAAAGCGG CCATTTTCCA CCATGATATT CGGCAAGCAG 
     6721 GCATCGCCAT GGGTCACGAC GAGATCCTCG CCGTCGGGCA TGCTCGCCTT GAGCCTGGCG 
     6781 AACAGTTCGG CTGGCGCGAG CCCCTGATGC TCTTCGTCCA GATCATCCTG ATCGACAAGA 
     6841 CCGGCTTCCA TCCGAGTACG TGCTCGCTCG ATGCGATGTT TCGCTTGGTG GTCGAATGGG 
     6901 CAGGTAGCCG GATCAAGCGT ATGCAGCCGC CGCATTGCAT CAGCCATGAT GGATACTTTC 
     6961 TCGGCAGGAG CAAGGTGAGA TGACAGGAGA TCCTGCCCCG GCACTTCGCC CAATAGCAGC 
     7021 CAGTCCCTTC CCGCTTCAGT GACAACGTCG AGCACAGCTG CGCAAGGAAC GCCCGTCGTG 
     7081 GCCAGCCACG ATAGCCGCGC TGCCTCGTCT TGCAGTTCAT TCAGGGCACC GGACAGGTCG 
     7141 GTCTTGACAA AAAGAACCGG GCGCCCCTGC GCTGACAGCC GGAACACGGC GGCATCAGAG 
     7201 CAGCCGATTG TCTGTTGTGC CCAGTCATAG CCGAATAGCC TCTCCACCCA AGCGGCCGGA 
     7261 GAACCTGCGT GCAATCCATC TTGTTCAATC ATGCGAAACG ATCCTCATCC TGTCTCTTGA 
     7321 TCGATCTTTG CAAAAGCCTA GGCCTCCAAA AAAGCCTCCT CACTACTTCT GGAATAGCTC 
     7381 AGAGGCCGAG GCGGCCTCGG CCTCTGCATA AATAAAAAAA ATTAGTCAGC CATGGGGCGG 
     7441 AGAATGGGCG GAACTGGGCG GAGTTAGGGG CGGGATGGGC GGAGTTAGGG GCGGGACTAT 
     7501 GGTTGCTGAC TAATTGAGAT GCATGCTTTG CATACTTCTG CCTGCTGGGG AGCCTGGGGA 
     7561 CTTTCCACAC CTGGTTGCTG ACTAATTGAG ATGCATGCTT TGCATACTTC TGCCTGCTGG 
     7621 GGAGCCTGGG GACTTTCCAC ACCCTAACTG ACACACATTC CACAGCTGGT TCTTTCCGCC 
     7681 TCAGGACTCT TCCTTTTTCA ATATTATTGA AGCATTTATC AGGGTTATTG TCTCATGAGC 
     7741 GGATACATAT TTGAATGTAT TTAGAAAAAT AAACAAATAG GGGTTCCGCG CACATTTCCC 
     7801 CGAAAAGTGC CACCTGACGC GCCCTGTAGC GGCGCATTAA GCGCGGCGGG TGTGGTGGTT 
     7861 ACGCGCAGCG TGACCGCTAC ACTTGCCAGC GCCCTAGCGC CCGCTCCTTT CGCTTTCTTC 
     7921 CCTTCCTTTC TCGCCACGTT CGCCGGCTTT CCCCGTCAAG CTCTAAATCG GGGGCTCCCT 
     7981 TTAGGGTTCC GATTTAGTGC TTTACGGCAC CTCGACCCCA AAAAACTTGA TTAGGGTGAT 
     8041 GGTTCACGTA GTGGGCCATC GCCCTGATAG ACGGTTTTTC GCCCTTTGAC GTTGGAGTCC 
     8101 ACGTTCTTTA ATAGTGGACT CTTGTTCCAA ACTGGAACAA CACTCAACCC TATCTCGGTC 
     8161 TATTCTTTTG ATTTATAAGG GATTTTGCCG ATTTCGGCCT ATTGGTTAAA AAATGAGCTG 
     8221 ATTTAACAAA AATTTAACGC GAATTTT
//