LOCUS       RC212783                6762 bp    DNA     circular  SYN 
DEFINITION  DTNB (Myc-DDK-tagged)-Human dystrobrevin, beta (DTNB), transcript variant 1
ACCESSION   NM_021907
VERSION     NM_021907.3
SOURCE      synthetic construct 
FEATURES    Location/Qualifiers 
     misc_feature    201..924 
                     /label="CMV promoter"  
     misc_feature    839..856 
                     /label="Restriction Enzyme"  
     misc_feature    953..971 
                     /label="T7 promoter"  
     misc_feature    2919..3010 
                     /label="Myc-DDK"  
     misc_feature    3040..3625 
                     /label="PolyA signal"  
     misc_feature    3097..3116 
                     /label="Restriction Enzyme"  
     misc_feature    3901..4573 
                     /label="ColE1"  
     misc_feature    5003..5797 
                     /label="Kan/Neo"  
     misc_feature    5832..6134 
                     /label="SV40 ori"  
     misc_feature    6220..6697 
                     /label="f1 ori"  
     misc_feature    1020..1028 
                     /label="Restriction Enzyme"  
     misc_feature    2901..2907 
                     /label="Restriction Enzyme"  
     misc_feature    1020..2901 
                     /label="DTNB"  
ORIGIN                      
        1 AACAAAATAT TAACGCTTAC AATTTCCATT CGCCATTCAG GCTGCGCAAC TGTTGGGAAG 
       61 GGCGATCGGT GCGGGCCTCT TCGCTATTAC GCCAGCTGGC GAAAGGGGGA TGTGCTGCAA 
      121 GGCGATTAAG TTGGGTAACG CCAGGGTTTT CCCAGTCACG ACGTTGTAAA ACGACGGCCA 
      181 GTGCCAAGCT GATCTATACA TTGAATCAAT ATTGGCAATT AGCCATATTA GTCATTGGTT 
      241 ATATAGCATA AATCAATATT GGCTATTGGC CATTGCATAC GTTGTATCTA TATCATAATA 
      301 TGTACATTTA TATTGGCTCA TGTCCAATAT GACCGCCATG TTGACATTGA TTATTGACTA 
      361 GTTATTAATA GTAATCAATT ACGGGGTCAT TAGTTCATAG CCCATATATG GAGTTCCGCG 
      421 TTACATAACT TACGGTAAAT GGCCCGCCTG GCTGACCGCC CAACGACCCC CGCCCATTGA 
      481 CGTCAATAAT GACGTATGTT CCCATAGTAA CGCCAATAGG GACTTTCCAT TGACGTCAAT 
      541 GGGTGGAGTA TTTACGGTAA ACTGCCCACT TGGCAGTACA TCAAGTGTAT CATATGCCAA 
      601 GTCCGCCCCC TATTGACGTC AATGACGGTA AATGGCCCGC CTGGCATTAT GCCCAGTACA 
      661 TGACCTTACG GGACTTTCCT ACTTGGCAGT ACATCTACGT ATTAGTCATC GCTATTACCA 
      721 TGGTGATGCG GTTTTGGCAG TACACCAATG GGCGTGGATA GCGGTTTGAC TCACGGGGAT 
      781 TTCCAAGTCT CCACCCCATT GACGTCAATG GGAGTTTGTT TTGGCACCAA AATCAACGGG 
      841 ACTTTCCAAA ATGTCGTAAT AACCCCGCCC CGTTGACGCA AATGGGCGGT AGGCGTGTAC 
      901 GGTGGGAGGT CTATATAAGC AGAGCTCGTT TAGTGAACCG TCAGAATTTT GTAATACGAC 
      961 TCACTATAGG GCGGCCGGGA ATTCGTCGAC TGGATCCGGT ACCGAGGAGA TCTGCCGCCG 
     1021 CGATCGCCAT GATTGAGGAA AGTGGGAACA AGCGGAAGAC CATGGCAGAG AAGAGGCAGC 
     1081 TGTTCATAGA AATGCGTGCT CAGAATTTTG ATGTCATACG ACTATCAACT TACAGAACAG 
     1141 CCTGCAAATT ACGATTTGTA CAAAAACGAT GCAACCTTCA TCTTGTTGAT ATCTGGAACA 
     1201 TGATTGAAGC CTTCCGAGAC AATGGCCTTA ATACACTGGA CCATACCACC GAGATCAGTG 
     1261 TGTCCCGCCT CGAAACTGTC ATCTCCTCCA TCTACTATCA GTTGAACAAG CGCCTTCCTT 
     1321 CTACTCACCA AATTAGTGTG GAACAATCTA TCAGCCTCCT CCTCAACTTT ATGATTGCTG 
     1381 CATATGACAG TGAGGGCCGA GGCAAGTTGA CGGTATTTTC AGTTAAAGCT ATGTTAGCAA 
     1441 CCATGTGTGG TGGAAAAATG CTGGACAAAT TGAGATATGT TTTCTCCCAG ATGTCAGATT 
     1501 CCAATGGCTT AATGATATTT AGCAAGTTTG ACCAGTTTCT GAAGGAAGTT CTGAAGCTCC 
     1561 CAACAGCTGT CTTTGAAGGG CCATCTTTTG GTTACACAGA GCACTCAGTC CGCACCTGTT 
     1621 TTCCACAGCA GAGAAAGATA ATGCTAAATA TGTTTTTAGA CACAATGATG GCTGACCCTC 
     1681 CTCCCCAGTG CCTTGTCTGG CTACCTCTCA TGCACAGGCT TGCCCATGTT GAGAATGTCT 
     1741 TCCATCCCGT GGAGTGCTCC TACTGCCGAT GTGAGAGTAT GATGGGTTTC CGGTACCGAT 
     1801 GCCAGCAGTG CCACAACTAT CAGCTCTGCC AGAATTGCTT TTGGCGTGGC CATGCCGGCG 
     1861 GCCCTCACAG CAACCAGCAC CAGATGAAGG AGCATTCCTC TTGGAAATCT CCTGCAAAGA 
     1921 AGCTGAGCCA TGCAATTAGT AAATCTTTGG GGTGTGTACC CACGAGAGAA CCCCCGCATC 
     1981 CTGTTTTTCC TGAGCAACCA GAGAAACCAC TTGACCTTGC ACATATAGTT CCTCCTCGCC 
     2041 CTCTGACTAA TATGAATGAC ACCATGGTTA GCCACATGTC CTCTGGAGTG CCCACTCCCA 
     2101 CCAAGAGGTT ACAGTATAGC CAGGATATAC CCAGTCACTT GGCCGATGAG CATGCGCTGA 
     2161 TAGCCTCCTA TGTGGCCCGT CTGCAGCACT GTGCACGTGT TCTGGACAGT CCTAGCCGAC 
     2221 TGGATGAGGA ACACCGTCTT ATAGCTCGCT ATGCTGCCCG GCTGGCTGCA GAAGCAGGAA 
     2281 ACGTGACTCG TCCTCCCACT GACTTGAGCT TTAACTTTGA TGCCAACAAA CAACAAAGAC 
     2341 AGCTTATTGC AGAACTGGAA AACAAAAACA GAGAGATCCT GCAGGAGATT CAGCGTCTCC 
     2401 GCCTGGAACA CGAGCAGGCC TCCCAGCCCA CCCCTGAGAA GGCACAGCAG AACCCCACGC 
     2461 TGCTGGCAGA GCTGCGGCTG CTGAGGCAAA GGAAGGATGA ACTGGAGCAG AGGATGTCGG 
     2521 CCCTGCAGGA GAGCAGGCGG GAGCTGATGG TCCAGCTGGA AGAGCTGATG AAGTTGCTGA 
     2581 AGGAGGAAGA GCAAAAGCAG GCAGCTCAGG CCACAGGGTC ACCACATACA TCGCCCACCC 
     2641 ATGGAGGCGG CCGGCCAATG CCCATGCCAG TGCGCTCCAC GTCTGCCGGC TCCACCCCCA 
     2701 CCCACTGTCC GCAGGACTCG CTGAGCGGAG TCGGGGGAGA CGTGCAGGAG GCCTTCGCAC 
     2761 AAGGTACGAG GAGAAACCTC CGCAATGACC TGCTGGTGGC AGCTGACTCC ATCACCAACA 
     2821 CCATGTCATC CCTGGTGAAG GAGCTCCATT CAGCAGAGGA AGGTGCAGAG GAAGAAGAAG 
     2881 AGAAGATGCA GAATGGGAAA GACAGAGGTA CGCGTACGCG GCCGCTCGAG CAGAAACTCA 
     2941 TCTCAGAAGA GGATCTGGCA GCAAATGATA TCCTGGATTA CAAGGATGAC GACGATAAGG 
     3001 TTTAAACGGC CGGCCGCGGT CATAGCTGTT TCCTGAACAG ATCCCGGGTG GCATCCCTGT 
     3061 GACCCCTCCC CAGTGCCTCT CCTGGCCCTG GAAGTTGCCA CTCCAGTGCC CACCAGCCTT 
     3121 GTCCTAATAA AATTAAGTTG CATCATTTTG TCTGACTAGG TGTCCTTCTA TAATATTATG 
     3181 GGGTGGAGGG GGGTGGTATG GAGCAAGGGG CAAGTTGGGA AGACAACCTG TAGGGCCTGC 
     3241 GGGGTCTATT GGGAACCAAG CTGGAGTGCA GTGGCACAAT CTTGGCTCAC TGCAATCTCC 
     3301 GCCTCCTGGG TTCAAGCGAT TCTCCTGCCT CAGCCTCCCG AGTTGTTGGG ATTCCAGGCA 
     3361 TGCATGACCA GGCTCAGCTA ATTTTTGTTT TTTTGGTAGA GACGGGGTTT CACCATATTG 
     3421 GCCAGGCTGG TCTCCAACTC CTAATCTCAG GTGATCTACC CACCTTGGCC TCCCAAATTG 
     3481 CTGGGATTAC AGGCGTGAAC CACTGCTCCC TTCCCTGTCC TTCTGATTTT AAAATAACTA 
     3541 TACCAGCAGG AGGACGTCCA GACACAGCAT AGGCTACCTG GCCATGCCCA ACCGGTGGGA 
     3601 CATTTGAGTT GCTTGCTTGG CACTGTCCTC TCATGCGTTG GGTCCACTCA GTAGATGCCT 
     3661 GTTGAATTGG GTACGCGGCC AGCGGCGAGC GGTATCAGCT CACTCAAAGG CGGTAATACG 
     3721 GTTATCCACA GAATCAGGGG ATAACGCAGG AAAGAACATG TGAGCAAAAG GCCAGCAAAA 
     3781 GGCCAGGAAC CGTAAAAAGG CCGCGTTGCT GGCGTTTTTC CATAGGCTCC GCCCCCCTGA 
     3841 CGAGCATCAC AAAAATCGAC GCTCAAGTCA GAGGTGGCGA AACCCGACAG GACTATAAAG 
     3901 ATACCAGGCG TTTCCCCCTG GAAGCTCCCT CGTGCGCTCT CCTGTTCCGA CCCTGCCGCT 
     3961 TACCGGATAC CTGTCCGCCT TTCTCCCTTC GGGAAGCGTG GCGCTTTCTC ATAGCTCACG 
     4021 CTGTAGGTAT CTCAGTTCGG TGTAGGTCGT TCGCTCCAAG CTGGGCTGTG TGCACGAACC 
     4081 CCCCGTTCAG CCCGACCGCT GCGCCTTATC CGGTAACTAT CGTCTTGAGT CCAACCCGGT 
     4141 AAGACACGAC TTATCGCCAC TGGCAGCAGC CACTGGTAAC AGGATTAGCA GAGCGAGGTA 
     4201 TGTAGGCGGT GCTACAGAGT TCTTGAAGTG GTGGCCTAAC TACGGCTACA CTAGAAGAAC 
     4261 AGTATTTGGT ATCTGCGCTC TGCTGAAGCC AGTTACCTTC GGAAAAAGAG TTGGTAGCTC 
     4321 TTGATCCGGC AAACAAACCA CCGCTGGTAG CGGTGGTTTT TTTGTTTGCA AGCAGCAGAT 
     4381 TACGCGCAGA AAAAAAGGAT CTCAAGAAGA TCCTTTGATC TTTTCTACGG GGTCTGACGC 
     4441 TCAGTGGAAC GAAAACTCAC GTTAAGGGAT TTTGGTCATG AGATTATCAA AAAGGATCTT 
     4501 CACCTAGATC CTTTTAAATT AAAAATGAAG TTTTAAATCA ATCTAAAGTA TATATGAGTA 
     4561 ACCTGAGGCT ATGGCAGGGC CTGCCGCCCC GACGTTGGCT GCGAGCCCTG GGCCTTCACC 
     4621 CGAACTTGGG GGGTGGGGTG GGGAAAAGGA AGAAACGCGG GCGTATTGGC CCCAATGGGG 
     4681 TCTCGGTGGG GTATCGACAG AGTGCCAGCC CTGGGACCGA ACCCCGCGTT TATGAACAAA 
     4741 CGACCCAACA CCGTGCGTTT TATTCTGTCT TTTTATTGCC GTCATAGCGC GGGTTCCTTC 
     4801 CGGTATTGTC TCCTTCCGTG TTTCAGTTAG CCTCCCCCTA GGGTGGGCGA AGAACTCCAG 
     4861 CATGAGATCC CCGCGCTGGA GGATCATCCA GCCGGCGTCC CGGAAAACGA TTCCGAAGCC 
     4921 CAACCTTTCA TAGAAGGCGG CGGTGGAATC GAAATCTCGT GATGGCAGGT TGGGCGTCGC 
     4981 TTGGTCGGTC ATTTCGAACC CCAGAGTCCC GCTCAGAAGA ACTCGTCAAG AAGGCGATAG 
     5041 AAGGCGATGC GCTGCGAATC GGGAGCGGCG ATACCGTAAA GCACGAGGAA GCGGTCAGCC 
     5101 CATTCGCCGC CAAGCTCTTC AGCAATATCA CGGGTAGCCA ACGCTATGTC CTGATAGCGA 
     5161 TCCGCCACAC CCAGCCGGCC ACAGTCGATG AATCCAGAAA AGCGGCCATT TTCCACCATG 
     5221 ATATTCGGCA AGCAGGCATC GCCATGGGTC ACGACGAGAT CCTCGCCGTC GGGCATGCTC 
     5281 GCCTTGAGCC TGGCGAACAG TTCGGCTGGC GCGAGCCCCT GATGCTCTTC GTCCAGATCA 
     5341 TCCTGATCGA CAAGACCGGC TTCCATCCGA GTACGTGCTC GCTCGATGCG ATGTTTCGCT 
     5401 TGGTGGTCGA ATGGGCAGGT AGCCGGATCA AGCGTATGCA GCCGCCGCAT TGCATCAGCC 
     5461 ATGATGGATA CTTTCTCGGC AGGAGCAAGG TGAGATGACA GGAGATCCTG CCCCGGCACT 
     5521 TCGCCCAATA GCAGCCAGTC CCTTCCCGCT TCAGTGACAA CGTCGAGCAC AGCTGCGCAA 
     5581 GGAACGCCCG TCGTGGCCAG CCACGATAGC CGCGCTGCCT CGTCTTGCAG TTCATTCAGG 
     5641 GCACCGGACA GGTCGGTCTT GACAAAAAGA ACCGGGCGCC CCTGCGCTGA CAGCCGGAAC 
     5701 ACGGCGGCAT CAGAGCAGCC GATTGTCTGT TGTGCCCAGT CATAGCCGAA TAGCCTCTCC 
     5761 ACCCAAGCGG CCGGAGAACC TGCGTGCAAT CCATCTTGTT CAATCATGCG AAACGATCCT 
     5821 CATCCTGTCT CTTGATCGAT CTTTGCAAAA GCCTAGGCCT CCAAAAAAGC CTCCTCACTA 
     5881 CTTCTGGAAT AGCTCAGAGG CCGAGGCGGC CTCGGCCTCT GCATAAATAA AAAAAATTAG 
     5941 TCAGCCATGG GGCGGAGAAT GGGCGGAACT GGGCGGAGTT AGGGGCGGGA TGGGCGGAGT 
     6001 TAGGGGCGGG ACTATGGTTG CTGACTAATT GAGATGCATG CTTTGCATAC TTCTGCCTGC 
     6061 TGGGGAGCCT GGGGACTTTC CACACCTGGT TGCTGACTAA TTGAGATGCA TGCTTTGCAT 
     6121 ACTTCTGCCT GCTGGGGAGC CTGGGGACTT TCCACACCCT AACTGACACA CATTCCACAG 
     6181 CTGGTTCTTT CCGCCTCAGG ACTCTTCCTT TTTCAATATT ATTGAAGCAT TTATCAGGGT 
     6241 TATTGTCTCA TGAGCGGATA CATATTTGAA TGTATTTAGA AAAATAAACA AATAGGGGTT 
     6301 CCGCGCACAT TTCCCCGAAA AGTGCCACCT GACGCGCCCT GTAGCGGCGC ATTAAGCGCG 
     6361 GCGGGTGTGG TGGTTACGCG CAGCGTGACC GCTACACTTG CCAGCGCCCT AGCGCCCGCT 
     6421 CCTTTCGCTT TCTTCCCTTC CTTTCTCGCC ACGTTCGCCG GCTTTCCCCG TCAAGCTCTA 
     6481 AATCGGGGGC TCCCTTTAGG GTTCCGATTT AGTGCTTTAC GGCACCTCGA CCCCAAAAAA 
     6541 CTTGATTAGG GTGATGGTTC ACGTAGTGGG CCATCGCCCT GATAGACGGT TTTTCGCCCT 
     6601 TTGACGTTGG AGTCCACGTT CTTTAATAGT GGACTCTTGT TCCAAACTGG AACAACACTC 
     6661 AACCCTATCT CGGTCTATTC TTTTGATTTA TAAGGGATTT TGCCGATTTC GGCCTATTGG 
     6721 TTAAAAAATG AGCTGATTTA ACAAAAATTT AACGCGAATT TT
//