LOCUS       RC212739                9150 bp    DNA     circular  SYN 
DEFINITION  SSH2 (Myc-DDK-tagged)-Human slingshot homolog 2 (Drosophila) (SSH2)
ACCESSION   NM_033389
VERSION     NM_033389.2
SOURCE      synthetic construct 
FEATURES    Location/Qualifiers 
     misc_feature    201..924 
                     /label="CMV promoter"  
     misc_feature    839..856 
                     /label="Restriction Enzyme"  
     misc_feature    953..971 
                     /label="T7 promoter"  
     misc_feature    5307..5398 
                     /label="Myc-DDK"  
     misc_feature    5428..6013 
                     /label="PolyA signal"  
     misc_feature    5485..5504 
                     /label="Restriction Enzyme"  
     misc_feature    6289..6961 
                     /label="ColE1"  
     misc_feature    7391..8185 
                     /label="Kan/Neo"  
     misc_feature    8220..8522 
                     /label="SV40 ori"  
     misc_feature    8608..9085 
                     /label="f1 ori"  
     misc_feature    1020..1028 
                     /label="Restriction Enzyme"  
     misc_feature    5289..5295 
                     /label="Restriction Enzyme"  
     misc_feature    1020..5289 
                     /label="SSH2"  
ORIGIN                      
        1 AACAAAATAT TAACGCTTAC AATTTCCATT CGCCATTCAG GCTGCGCAAC TGTTGGGAAG 
       61 GGCGATCGGT GCGGGCCTCT TCGCTATTAC GCCAGCTGGC GAAAGGGGGA TGTGCTGCAA 
      121 GGCGATTAAG TTGGGTAACG CCAGGGTTTT CCCAGTCACG ACGTTGTAAA ACGACGGCCA 
      181 GTGCCAAGCT GATCTATACA TTGAATCAAT ATTGGCAATT AGCCATATTA GTCATTGGTT 
      241 ATATAGCATA AATCAATATT GGCTATTGGC CATTGCATAC GTTGTATCTA TATCATAATA 
      301 TGTACATTTA TATTGGCTCA TGTCCAATAT GACCGCCATG TTGACATTGA TTATTGACTA 
      361 GTTATTAATA GTAATCAATT ACGGGGTCAT TAGTTCATAG CCCATATATG GAGTTCCGCG 
      421 TTACATAACT TACGGTAAAT GGCCCGCCTG GCTGACCGCC CAACGACCCC CGCCCATTGA 
      481 CGTCAATAAT GACGTATGTT CCCATAGTAA CGCCAATAGG GACTTTCCAT TGACGTCAAT 
      541 GGGTGGAGTA TTTACGGTAA ACTGCCCACT TGGCAGTACA TCAAGTGTAT CATATGCCAA 
      601 GTCCGCCCCC TATTGACGTC AATGACGGTA AATGGCCCGC CTGGCATTAT GCCCAGTACA 
      661 TGACCTTACG GGACTTTCCT ACTTGGCAGT ACATCTACGT ATTAGTCATC GCTATTACCA 
      721 TGGTGATGCG GTTTTGGCAG TACACCAATG GGCGTGGATA GCGGTTTGAC TCACGGGGAT 
      781 TTCCAAGTCT CCACCCCATT GACGTCAATG GGAGTTTGTT TTGGCACCAA AATCAACGGG 
      841 ACTTTCCAAA ATGTCGTAAT AACCCCGCCC CGTTGACGCA AATGGGCGGT AGGCGTGTAC 
      901 GGTGGGAGGT CTATATAAGC AGAGCTCGTT TAGTGAACCG TCAGAATTTT GTAATACGAC 
      961 TCACTATAGG GCGGCCGGGA ATTCGTCGAC TGGATCCGGT ACCGAGGAGA TCTGCCGCCG 
     1021 CGATCGCCAT GGCTTTGGTC ACGGTCCAGC GGTCACCTAC CCCCAGCACC ACCTCCAGCC 
     1081 CCTGCGCCTC GGAGGCAGAC AGTGGGGAGG AAGAATGCCG GTCACAGCCC AGGAGCATCA 
     1141 GCGAGAGCTT TCTAACTGTC AAAGGTGCTG CCCTTTTTCT ACCACGGGGA AATGGCTCAT 
     1201 CCACACCAAG AATCAGCCAC AGACGGAACA AGCATGCAGG CGATCTCCAA CAGCATCTCC 
     1261 AAGCAATGTT CATTTTACTC CGCCCAGAAG ACAACATCAG ACTGGCTGTA AGACTGGAAA 
     1321 GTACTTACCA GAATCGAACA CGCTATATGG TAGTGGTTTC AACTAATGGT AGACAAGACA 
     1381 CTGAAGAAAG CATCGTCCTA GGAATGGATT TCTCCTCTAA TGACAGTAGC ACTTGTACCA 
     1441 TGGGCTTAGT TTTGCCTCTC TGGAGCGACA CGCTAATTCA TTTGGATGGT GATGGTGGGT 
     1501 TCAGTGTATC GACGGATAAC AGAGTTCACA TATTCAAACC TGTATCTGTG CAGGCAATGT 
     1561 GGTCTGCACT ACAGAGCTTA CACAAGGCTT GTGAAGTCGC CAGAGCGCAT AACTACTACC 
     1621 CAGGCAGCCT ATTTCTCACT TGGGTGAGTT ATTATGAGAG CCATATCAAC TCAGATCAAT 
     1681 CCTCAGTCAA TGAATGGAAT GCAATGCAAG ATGTACAGTC CCACCGGCCC GACTCTCCAG 
     1741 CTCTCTTCAC CGACATACCT ACTGAACGTG AACGAACAGA AAGGCTAATT AAAACCAAAT 
     1801 TAAGGGAGAT CATGATGCAG AAGGATTTGG AGAATATTAC ATCCAAAGAG ATAAGAACAG 
     1861 AGTTGGAAAT GCAAATGGTG TGCAACTTGC GGGAATTCAA GGAATTTATA GACAATGAAA 
     1921 TGATAGTGAT CCTTGGTCAA ATGGATAGCC CTACACAGAT ATTTGAGCAT GTGTTCCTGG 
     1981 GCTCAGAATG GAATGCCTCC AACTTAGAGG ACTTACAGAA CCGAGGGGTA CGGTATATCT 
     2041 TGAATGTCAC TCGAGAGATA GATAACTTCT TCCCAGGAGT CTTTGAGTAT CATAACATTC 
     2101 GGGTATATGA TGAAGAGGCA ACGGATCTCC TGGCGTACTG GAATGACACT TACAAATTCA 
     2161 TCTCTAAAGC AAAGAAACAT GGATCTAAAT GCCTTGTGCA CTGCAAAATG GGGGTGAGTC 
     2221 GCTCAGCCTC CACCGTGATT GCCTATGCAA TGAAGGAATA TGGCTGGAAT CTGGACCGAG 
     2281 CCTATGACTA TGTGAAAGAA AGACGAACGG TAACCAAGCC CAACCCAAGC TTCATGAGAC 
     2341 AACTGGAAGA GTATCAGGGG ATCTTGCTGG CAAGCAAACA GCGGCATAAC AAACTATGGA 
     2401 GATCTCATTC AGATAGTGAC CTCTCAGACC ACCACGAACC CATCTGCAAA CCTGGGCTAG 
     2461 AACTCAACAA GAAGGATATC ACCACCTCAG CAGACCAGAT TGCTGAGGTG AAGACCATGG 
     2521 AGAGTCACCC ACCCATACCT CCTGTCTTTG TGGAACATAT GGTCCCACAA GATGCAAATC 
     2581 AGAAAGGCCT GTGTACCAAA GAAAGAATGA TCTGCTTGGA GTTTACTTCT AGGGAATTTC 
     2641 ATGCTGGACA GATTGAGGAT GAATTAAACT TAAATGACAT CAATGGATGC TCATCAGGGT 
     2701 GTTGTCTGAA TGAATCAAAA TTTCCTCTTG ACAATTGCCA TGCATCCAAA GCCTTAATTC 
     2761 AGCCTGGACA TGTCCCAGAA ATGGCCAACA AGTTTCCAGA CTTAACAGTG GAAGATTTGG 
     2821 AGACAGATGC ACTGAAAGCA GACATGAATG TCCACCTACT GCCTATGGAA GAATTGACAT 
     2881 CTCCACTGAA AGACCCCCCC ATGTCCCCTG ATCCTGAGTC ACCAAGCCCC CAACCCAGTT 
     2941 GCCAGACTGA AATCTCAGAT TTCAGTACAG ATCGCATTGA CTTTTTTAGT GCCCTAGAGA 
     3001 AGTTTGTGGA GCTCTCCCAA GAAACCCGGT CACGATCTTT TTCCCATTCA AGGATGGAGG 
     3061 AACTGGGTGG AGGAAGGAAT GAGAGCTGTC GACTGTCAGT GGTAGAAGTA GCCCCTTCCA 
     3121 AAGTGACAGC TGATGACCAG AGAAGCAGCT CTTTGAGTAA TACTCCCCAT GCATCAGAAG 
     3181 AATCTTCAAT GGATGAGGAA CAGTCAAAGG CAATTTCAGA ACTGGTCAGC CCAGACATCT 
     3241 TCATGCAGTC TCACTCGGAA AATGCAATTT CAGTCAAAGA AATTGTCACT GAAATTGAGT 
     3301 CCATCAGTCA AGGAGTTGGG CAGATTCAAC TGAAAGGAGA CATCTTACCC AACCCATGCC 
     3361 ATACACCAAA GAAGAACAGC ATCCATGAGC TGCTCCTTGA GAGGGCCCAG ACTCCAGAGA 
     3421 ACAAACCTGG ACATATGGAG CAAGATGAGG ACTCCTGCAC AGCCCAGCCT GAACTAGCCA 
     3481 AAGACTCAGG GATGTGCAAC CCAGAAGGCT GCCTAACCAC ACACTCATCT ATAGCAGACT 
     3541 TGGAAGAAGG GGAACCAGCT GAGGGGGAAC AAGAGCTCCA GGGCTCAGGG ATGCACCCAG 
     3601 GTGCCAAGTG GTACCCTGGG TCTGTGAGGC GAGCCACCTT GGAGTTCGAA GAGCGCTTAC 
     3661 GGCAGGAGCA AGAGCATCAT GGTGCTGCCC CAACATGTAC CTCATTGTCC ACTCGTAAGA 
     3721 ATTCAAAGAA TGATTCTTCT GTGGCAGACC TAGCACCAAA AGGGAAAAGT GATGAAGCCC 
     3781 CCCCAGAACA TTCATTTGTC CTCAAGGAAC CAGAAATGAG CAAAGGCAAA GGGAAATACA 
     3841 GTGGGTCTGA GGCTGGCTCA CTGTCCCATT CTGAGCAGAA TGCCACTGTT CCAGCTCCCA 
     3901 GGGTGCTGGA GTTTGACCAC TTGCCAGATC CTCAGGAGGG CCCAGGGTCA GATACTGGAA 
     3961 CACAGCAGGA AGGAGTCCTG AAGGATCTGA GGACTGTGAT TCCATACCAG GAGTCTGAAA 
     4021 CACAAGCAGT CCCTCTTCCC CTTCCCAAGA GGGTAGAAAT CATTGAATAT ACCCACATAG 
     4081 TTACATCACC CAATCACACT GGGCCAGGGA GTGAAATAGC CACCAGTGAG AAGAGCGGAG 
     4141 AGCAAGGGCT GAGGAAAGTG AACATGGAAA AATCTGTCAC TGTGCTCTGC ACACTGGATG 
     4201 AAAATCTAAA CAGGACTCTG GACCCCAACC AGGTTTCTCT GCACCCCCAA GTGCTACCTC 
     4261 TGCCTCATTC TTCCTCCCCT GAGCACAACA GACCCACTGA CCATCCAACC TCCATCCTGA 
     4321 GTAGCCCTGA AGACAGAGGC AGCAGCCTGT CCACAGCCCT GGAGACAGCA GCACCTTTTG 
     4381 TCAGTCATAC AACCCATTTA CTGTCTGCCA GTTTGGATTA CCTGCATCCC CAGACTATGG 
     4441 TTCACCTGGA GGGCTTCACA GAGCAGAGCA GCACTACAGA TGAGCCCTCT GCAGAACAGG 
     4501 TTAGCTGGGA AGAAAGTCAG GAGAGCCCTC TCTCCAGTGG CAGTGAGGTG CCATATAAGG 
     4561 ACTCCCAGCT AAGTAGCGCA GACCTAAGTT TAATTAGCAA ACTTGGTGAC AACACTGGGG 
     4621 AGTTACAGGA GAAAATGGAC CCATTGCCTG TAGCCTGTCG ACTCCCACAT AGCTCTAGTA 
     4681 GTGAAAACAT AAAGAGTCTC AGCCACAGCC CCGGTGTGGT GAAGGAGCGT GCTAAAGAAA 
     4741 TCGAGTCTCG AGTGGTTTTC CAGGCAGGGC TCACCAAACC ATCCCAAATG AGGCGCTCAG 
     4801 CTTCTCTCGC CAAATTAGGT TACTTGGACC TCTGTAAAGA CTGCTTACCA GAGAGGGAGC 
     4861 CTGCCTCCTG TGAATCCCCT CATCTCAAAC TGCTTCAGCC TTTCCTCAGA ACAGACTCAG 
     4921 GCATGCACGC GATGGAGGAC CAAGAGTCCC TAGAAAACCC AGGTGCCCCC CACAACCCAG 
     4981 AGCCCACCAA GTCTTTTGTA GAACAACTCA CAACAACAGA GTGTATTGTG CAGAGCAAGC 
     5041 CAGTGGAGAG GCCCCTTGTG CAGTATGCCA AAGAATTTGG TTCTAGTCAG CAGTATTTGC 
     5101 TCCCCAGGGC AGGACTTGAA TTGACTAGTT CTGAAGGAGG CCTTCCCGTG CTACAGACCC 
     5161 AGGGACTGCA GTGTGCATGC CCAGCTCCAG GGCTGGCCGT GGCACCCCGT CAGCAACACG 
     5221 GCAGAACTCA CCCCCTTAGG AGACTGAAAA AGGCAAATGA CAAAAAACGG ACAACCAACC 
     5281 CCTTCTATAA TACCATGACG CGTACGCGGC CGCTCGAGCA GAAACTCATC TCAGAAGAGG 
     5341 ATCTGGCAGC AAATGATATC CTGGATTACA AGGATGACGA CGATAAGGTT TAAACGGCCG 
     5401 GCCGCGGTCA TAGCTGTTTC CTGAACAGAT CCCGGGTGGC ATCCCTGTGA CCCCTCCCCA 
     5461 GTGCCTCTCC TGGCCCTGGA AGTTGCCACT CCAGTGCCCA CCAGCCTTGT CCTAATAAAA 
     5521 TTAAGTTGCA TCATTTTGTC TGACTAGGTG TCCTTCTATA ATATTATGGG GTGGAGGGGG 
     5581 GTGGTATGGA GCAAGGGGCA AGTTGGGAAG ACAACCTGTA GGGCCTGCGG GGTCTATTGG 
     5641 GAACCAAGCT GGAGTGCAGT GGCACAATCT TGGCTCACTG CAATCTCCGC CTCCTGGGTT 
     5701 CAAGCGATTC TCCTGCCTCA GCCTCCCGAG TTGTTGGGAT TCCAGGCATG CATGACCAGG 
     5761 CTCAGCTAAT TTTTGTTTTT TTGGTAGAGA CGGGGTTTCA CCATATTGGC CAGGCTGGTC 
     5821 TCCAACTCCT AATCTCAGGT GATCTACCCA CCTTGGCCTC CCAAATTGCT GGGATTACAG 
     5881 GCGTGAACCA CTGCTCCCTT CCCTGTCCTT CTGATTTTAA AATAACTATA CCAGCAGGAG 
     5941 GACGTCCAGA CACAGCATAG GCTACCTGGC CATGCCCAAC CGGTGGGACA TTTGAGTTGC 
     6001 TTGCTTGGCA CTGTCCTCTC ATGCGTTGGG TCCACTCAGT AGATGCCTGT TGAATTGGGT 
     6061 ACGCGGCCAG CGGCGAGCGG TATCAGCTCA CTCAAAGGCG GTAATACGGT TATCCACAGA 
     6121 ATCAGGGGAT AACGCAGGAA AGAACATGTG AGCAAAAGGC CAGCAAAAGG CCAGGAACCG 
     6181 TAAAAAGGCC GCGTTGCTGG CGTTTTTCCA TAGGCTCCGC CCCCCTGACG AGCATCACAA 
     6241 AAATCGACGC TCAAGTCAGA GGTGGCGAAA CCCGACAGGA CTATAAAGAT ACCAGGCGTT 
     6301 TCCCCCTGGA AGCTCCCTCG TGCGCTCTCC TGTTCCGACC CTGCCGCTTA CCGGATACCT 
     6361 GTCCGCCTTT CTCCCTTCGG GAAGCGTGGC GCTTTCTCAT AGCTCACGCT GTAGGTATCT 
     6421 CAGTTCGGTG TAGGTCGTTC GCTCCAAGCT GGGCTGTGTG CACGAACCCC CCGTTCAGCC 
     6481 CGACCGCTGC GCCTTATCCG GTAACTATCG TCTTGAGTCC AACCCGGTAA GACACGACTT 
     6541 ATCGCCACTG GCAGCAGCCA CTGGTAACAG GATTAGCAGA GCGAGGTATG TAGGCGGTGC 
     6601 TACAGAGTTC TTGAAGTGGT GGCCTAACTA CGGCTACACT AGAAGAACAG TATTTGGTAT 
     6661 CTGCGCTCTG CTGAAGCCAG TTACCTTCGG AAAAAGAGTT GGTAGCTCTT GATCCGGCAA 
     6721 ACAAACCACC GCTGGTAGCG GTGGTTTTTT TGTTTGCAAG CAGCAGATTA CGCGCAGAAA 
     6781 AAAAGGATCT CAAGAAGATC CTTTGATCTT TTCTACGGGG TCTGACGCTC AGTGGAACGA 
     6841 AAACTCACGT TAAGGGATTT TGGTCATGAG ATTATCAAAA AGGATCTTCA CCTAGATCCT 
     6901 TTTAAATTAA AAATGAAGTT TTAAATCAAT CTAAAGTATA TATGAGTAAC CTGAGGCTAT 
     6961 GGCAGGGCCT GCCGCCCCGA CGTTGGCTGC GAGCCCTGGG CCTTCACCCG AACTTGGGGG 
     7021 GTGGGGTGGG GAAAAGGAAG AAACGCGGGC GTATTGGCCC CAATGGGGTC TCGGTGGGGT 
     7081 ATCGACAGAG TGCCAGCCCT GGGACCGAAC CCCGCGTTTA TGAACAAACG ACCCAACACC 
     7141 GTGCGTTTTA TTCTGTCTTT TTATTGCCGT CATAGCGCGG GTTCCTTCCG GTATTGTCTC 
     7201 CTTCCGTGTT TCAGTTAGCC TCCCCCTAGG GTGGGCGAAG AACTCCAGCA TGAGATCCCC 
     7261 GCGCTGGAGG ATCATCCAGC CGGCGTCCCG GAAAACGATT CCGAAGCCCA ACCTTTCATA 
     7321 GAAGGCGGCG GTGGAATCGA AATCTCGTGA TGGCAGGTTG GGCGTCGCTT GGTCGGTCAT 
     7381 TTCGAACCCC AGAGTCCCGC TCAGAAGAAC TCGTCAAGAA GGCGATAGAA GGCGATGCGC 
     7441 TGCGAATCGG GAGCGGCGAT ACCGTAAAGC ACGAGGAAGC GGTCAGCCCA TTCGCCGCCA 
     7501 AGCTCTTCAG CAATATCACG GGTAGCCAAC GCTATGTCCT GATAGCGATC CGCCACACCC 
     7561 AGCCGGCCAC AGTCGATGAA TCCAGAAAAG CGGCCATTTT CCACCATGAT ATTCGGCAAG 
     7621 CAGGCATCGC CATGGGTCAC GACGAGATCC TCGCCGTCGG GCATGCTCGC CTTGAGCCTG 
     7681 GCGAACAGTT CGGCTGGCGC GAGCCCCTGA TGCTCTTCGT CCAGATCATC CTGATCGACA 
     7741 AGACCGGCTT CCATCCGAGT ACGTGCTCGC TCGATGCGAT GTTTCGCTTG GTGGTCGAAT 
     7801 GGGCAGGTAG CCGGATCAAG CGTATGCAGC CGCCGCATTG CATCAGCCAT GATGGATACT 
     7861 TTCTCGGCAG GAGCAAGGTG AGATGACAGG AGATCCTGCC CCGGCACTTC GCCCAATAGC 
     7921 AGCCAGTCCC TTCCCGCTTC AGTGACAACG TCGAGCACAG CTGCGCAAGG AACGCCCGTC 
     7981 GTGGCCAGCC ACGATAGCCG CGCTGCCTCG TCTTGCAGTT CATTCAGGGC ACCGGACAGG 
     8041 TCGGTCTTGA CAAAAAGAAC CGGGCGCCCC TGCGCTGACA GCCGGAACAC GGCGGCATCA 
     8101 GAGCAGCCGA TTGTCTGTTG TGCCCAGTCA TAGCCGAATA GCCTCTCCAC CCAAGCGGCC 
     8161 GGAGAACCTG CGTGCAATCC ATCTTGTTCA ATCATGCGAA ACGATCCTCA TCCTGTCTCT 
     8221 TGATCGATCT TTGCAAAAGC CTAGGCCTCC AAAAAAGCCT CCTCACTACT TCTGGAATAG 
     8281 CTCAGAGGCC GAGGCGGCCT CGGCCTCTGC ATAAATAAAA AAAATTAGTC AGCCATGGGG 
     8341 CGGAGAATGG GCGGAACTGG GCGGAGTTAG GGGCGGGATG GGCGGAGTTA GGGGCGGGAC 
     8401 TATGGTTGCT GACTAATTGA GATGCATGCT TTGCATACTT CTGCCTGCTG GGGAGCCTGG 
     8461 GGACTTTCCA CACCTGGTTG CTGACTAATT GAGATGCATG CTTTGCATAC TTCTGCCTGC 
     8521 TGGGGAGCCT GGGGACTTTC CACACCCTAA CTGACACACA TTCCACAGCT GGTTCTTTCC 
     8581 GCCTCAGGAC TCTTCCTTTT TCAATATTAT TGAAGCATTT ATCAGGGTTA TTGTCTCATG 
     8641 AGCGGATACA TATTTGAATG TATTTAGAAA AATAAACAAA TAGGGGTTCC GCGCACATTT 
     8701 CCCCGAAAAG TGCCACCTGA CGCGCCCTGT AGCGGCGCAT TAAGCGCGGC GGGTGTGGTG 
     8761 GTTACGCGCA GCGTGACCGC TACACTTGCC AGCGCCCTAG CGCCCGCTCC TTTCGCTTTC 
     8821 TTCCCTTCCT TTCTCGCCAC GTTCGCCGGC TTTCCCCGTC AAGCTCTAAA TCGGGGGCTC 
     8881 CCTTTAGGGT TCCGATTTAG TGCTTTACGG CACCTCGACC CCAAAAAACT TGATTAGGGT 
     8941 GATGGTTCAC GTAGTGGGCC ATCGCCCTGA TAGACGGTTT TTCGCCCTTT GACGTTGGAG 
     9001 TCCACGTTCT TTAATAGTGG ACTCTTGTTC CAAACTGGAA CAACACTCAA CCCTATCTCG 
     9061 GTCTATTCTT TTGATTTATA AGGGATTTTG CCGATTTCGG CCTATTGGTT AAAAAATGAG 
     9121 CTGATTTAAC AAAAATTTAA CGCGAATTTT 
//