LOCUS       RC212738                8622 bp    DNA     circular  SYN 
DEFINITION  NEDD4 (Myc-DDK-tagged)-Human neural precursor cell expressed, developmentally down-regulated 4 (NEDD4), transcript variant 2
ACCESSION   NM_198400
VERSION     NM_198400.1
SOURCE      synthetic construct 
FEATURES    Location/Qualifiers 
     misc_feature    201..924 
                     /label="CMV promoter"  
     misc_feature    839..856 
                     /label="Restriction Enzyme"  
     misc_feature    953..971 
                     /label="T7 promoter"  
     misc_feature    4779..4870 
                     /label="Myc-DDK"  
     misc_feature    4900..5485 
                     /label="PolyA signal"  
     misc_feature    4957..4976 
                     /label="Restriction Enzyme"  
     misc_feature    5761..6433 
                     /label="ColE1"  
     misc_feature    6863..7657 
                     /label="Kan/Neo"  
     misc_feature    7692..7994 
                     /label="SV40 ori"  
     misc_feature    8080..8557 
                     /label="f1 ori"  
     misc_feature    1020..1028 
                     /label="Restriction Enzyme"  
     misc_feature    4761..4767 
                     /label="Restriction Enzyme"  
     misc_feature    1020..4761 
                     /label="NEDD4"  
ORIGIN                      
        1 AACAAAATAT TAACGCTTAC AATTTCCATT CGCCATTCAG GCTGCGCAAC TGTTGGGAAG 
       61 GGCGATCGGT GCGGGCCTCT TCGCTATTAC GCCAGCTGGC GAAAGGGGGA TGTGCTGCAA 
      121 GGCGATTAAG TTGGGTAACG CCAGGGTTTT CCCAGTCACG ACGTTGTAAA ACGACGGCCA 
      181 GTGCCAAGCT GATCTATACA TTGAATCAAT ATTGGCAATT AGCCATATTA GTCATTGGTT 
      241 ATATAGCATA AATCAATATT GGCTATTGGC CATTGCATAC GTTGTATCTA TATCATAATA 
      301 TGTACATTTA TATTGGCTCA TGTCCAATAT GACCGCCATG TTGACATTGA TTATTGACTA 
      361 GTTATTAATA GTAATCAATT ACGGGGTCAT TAGTTCATAG CCCATATATG GAGTTCCGCG 
      421 TTACATAACT TACGGTAAAT GGCCCGCCTG GCTGACCGCC CAACGACCCC CGCCCATTGA 
      481 CGTCAATAAT GACGTATGTT CCCATAGTAA CGCCAATAGG GACTTTCCAT TGACGTCAAT 
      541 GGGTGGAGTA TTTACGGTAA ACTGCCCACT TGGCAGTACA TCAAGTGTAT CATATGCCAA 
      601 GTCCGCCCCC TATTGACGTC AATGACGGTA AATGGCCCGC CTGGCATTAT GCCCAGTACA 
      661 TGACCTTACG GGACTTTCCT ACTTGGCAGT ACATCTACGT ATTAGTCATC GCTATTACCA 
      721 TGGTGATGCG GTTTTGGCAG TACACCAATG GGCGTGGATA GCGGTTTGAC TCACGGGGAT 
      781 TTCCAAGTCT CCACCCCATT GACGTCAATG GGAGTTTGTT TTGGCACCAA AATCAACGGG 
      841 ACTTTCCAAA ATGTCGTAAT AACCCCGCCC CGTTGACGCA AATGGGCGGT AGGCGTGTAC 
      901 GGTGGGAGGT CTATATAAGC AGAGCTCGTT TAGTGAACCG TCAGAATTTT GTAATACGAC 
      961 TCACTATAGG GCGGCCGGGA ATTCGTCGAC TGGATCCGGT ACCGAGGAGA TCTGCCGCCG 
     1021 CGATCGCCAT GGCACAAAGC TTACGATTGC ACTTTGCAGC CAGAAGAAGC AATACTTACC 
     1081 CTTTGTCAGA AACCTCCGGA GATGACTTGG ATAGCCATGT TCACATGTGC TTCAAAAGAC 
     1141 CAACACGGAT TTCAACGTCT AACGTTGTTC AAATGAAGCT GACTCCCAGA CAGACTGCAC 
     1201 TAGCTCCGTT AATAAAGGAA AACGTTCAGT CTCAAGAAAG ATCATCTGTT CCCTCATCTG 
     1261 AAAATGTTAA TAAAAAGAGC AGCTGTCTAC AGATTTCACT ACAGCCAACA AGGTACAGTG 
     1321 GATATCTTCA GTCTAGCAAT GTCTTAGCTG ATAGTGATGA TGCTTCGTTT ACTTGTATCT 
     1381 TGAAGGATGG TATTTACAGT AGTGCTGTGG TCGATAATGA ATTGAATGCT GTGAATGATG 
     1441 GTCACCTTGT AAGCAGTCCA GCCATTTGTA GTGGTAGCCT TAGTAACTTT TCAACCAGTG 
     1501 ATAATGGGTC TTACAGCAGC AACGGTAGTG ATTTTGGGTC ATGTGCAAGT ATCACAAGTG 
     1561 GAGGTTCATA TACTAACAGT GTCATCAGTG ACAGTAGTAG TTATACTTTT CCACCAAGTG 
     1621 ATGATACTTT TTTGGGTGGA AACTTACCTT CTGACAGCAC CTCCAATAGA AGTGTGCCAA 
     1681 ACAGGAATAC TACTCCTTGT GAAATTTTTT CAAGAAGTAC AAGTACAGAT CCTTTTGTCC 
     1741 AGGATGACTT GGAACATGGA TTAGAGATTA TGAAATTGCC AGTGAGCAGG AACACAAAAA 
     1801 TTCCACTAAA ACGTTACTCC TCCTTAGTCA TTTTTCCTAG GAGTCCTTCA ACTACCCGAC 
     1861 CGACTTCTCC AACAAGTCTG TGTACTCTTC TGAGCAAAGG ATCCTATCAA ACTTCACACC 
     1921 AGTTTATTAT TTCTCCTAGT GAAATTGCAC ATAATGAGGA TGGCACTAGT GCTAAAGGAT 
     1981 TTCTTTCAAC AGCTGTCAAT GGACTTCGGT TATCTAAAAC AATTTGTACC CCCGGAGAAG 
     2041 TAAGAGACAT ACGGCCGCTT CACAGGAAGG GCTCGTTACA GAAGAAAATT GTTCTTTCGA 
     2101 ATAATACTCC CAGACAGACT GTCTGTGAAA AGTCATCTGA AGGATATTCT TGTGTTTCAG 
     2161 TGCATTTCAC CCAACGAAAA GCAGCTACAT TAGACTGTGA AACAACAAAT GGTGATTGTA 
     2221 AACCAGAAAT GTCAGAAATT AAGCTTAATT CTGATTCAGA GTATATTAAG CTCATGCATA 
     2281 GGACATCTGC ATGTTTGCCA TCCTCCCAAA ATGTAGATTG TCAAATAAAT ATCAATGGAG 
     2341 AATTGGAAAG ACCACATTCA CAGATGAACA AAAACCATGG TATTTTACGA AGAAGTATTT 
     2401 CATTGGGAGG AGCTTATCCA AATATTTCCT GTCTATCCAG CCTTAAGCAC AATTGTTCTA 
     2461 AAGGGGGACC ATCTCAGTTA CTCATAAAGT TTGCATCTGG AAATGAAGGT AAAGTGGATA 
     2521 ATTTATCAAG AGACAGCAAC AGAGATTGCA CAAATGAACT GTCTAATTCT TGCAAGCCTG 
     2581 GCTGGGTTGT TTTGGACCAA CCAGATGCTG CTTGCCATTT GCAGCAACAA CAAGAACCTT 
     2641 CTCCTCTACC TCCAGGGTGG GAAGAGAGGC AGGATATCCT TGGAAGGACC TATTATGTAA 
     2701 ACCATGAATC TAGAAGAACA CAGTGGAAAA GACCAACCCC TCAGGACAAC CTAACAGATG 
     2761 CTGAGAATGG CAACATTCAA CTGCAAGCAC AACGTGCATT TACCACCAGG CGGCAGATAT 
     2821 CCGAGGAAAC AGAAAGTGTT GACAACCGAG AGTCTTCCGA GAACTGGGAA ATTATAAGAG 
     2881 AAGATGAAGC CACCATGTAT AGCAACCAGG CCTTCCCATC ACCTCCACCG TCAAGTAACT 
     2941 TGGATGTTCC AACTCATCTT GCAGAAGAAT TGAATGCCAG ACTCACCATT TTTGGAAATT 
     3001 CAGCCGTGAG CCAGCCAGCA TCGAGCTCAA ATCATTCCAG CAGAAGAGGC AGCTTACAAG 
     3061 CCTATACTTT TGAGGAACAA CCTACACTTC CTGTGCTTTT GCCTACTTCA TCTGGATTAC 
     3121 CACCAGGTTG GGAAGAAAAA CAAGATGAAA GAGGAAGATC ATATTATGTA GATCACAATT 
     3181 CCAGAACGAC TACTTGGACA AAGCCCACTG TACAGGCCAC AGTGGAGACC AGTCAGCTGA 
     3241 CCTCAAGCCA GAGTTCTGCA GGCCCTCAAT CACAAGCCTC CACCAGTGAT TCAGGCCAGC 
     3301 AGGTGACCCA GCCATCTGAA ATTGAGCAAG GATTCCTTCC TAAAGGCTGG GAAGTCCGGC 
     3361 ATGCACCAAA TGGGAGGCCT TTCTTTATTG ACCACAACAC TAAAACCACC ACCTGGGAAG 
     3421 ATCCAAGATT GAAAATTCCA GCCCATCTGA GAGGAAAGAC ATCACTTGAT ACTTCCAATG 
     3481 ATCTAGGGCC TTTACCTCCA GGATGGGAAG AGAGAACTCA CACAGATGGA AGAATCTTCT 
     3541 ACATAAATCA CAATATAAAA AGAACACAAT GGGAAGATCC TCGGTTGGAG AATGTAGCAA 
     3601 TAACTGGACC AGCAGTGCCC TACTCCAGGG ATTACAAAAG AAAGTATGAG TTCTTCCGAA 
     3661 GAAAGTTGAA GAAGCAGAAT GACATTCCAA ACAAATTTGA AATGAAACTT CGCCGAGCAA 
     3721 CTGTTCTTGA AGACTCTTAC CGGAGAATTA TGGGTGTCAA GAGAGCAGAC TTCCTGAAGG 
     3781 CTCGACTGTG GATTGAGTTT GATGGTGAAA AGGGATTGGA TTATGGAGGA GTTGCCAGAG 
     3841 AATGGTTCTT CCTGATCTCA AAGGAAATGT TTAACCCTTA TTATGGGTTG TTTGAATATT 
     3901 CTGCTACGGA CAATTATACC CTACAGATAA ATCCAAACTC TGGATTGTGT AACGAAGATC 
     3961 ACCTCTCTTA CTTCAAGTTT ATTGGTCGGG TAGCTGGAAT GGCAGTTTAT CATGGCAAAC 
     4021 TGTTGGATGG TTTTTTCATC CGCCCATTTT ACAAGATGAT GCTTCACAAA CCAATAACCC 
     4081 TTCATGATAT GGAATCTGTG GATAGTGAAT ATTACAATTC CCTAAGATGG ATTCTTGAAA 
     4141 ATGACCCAAC AGAATTGGAC CTCAGGTTTA TCATAGATGA AGAACTTTTT GGACAGACAC 
     4201 ATCAACATGA GCTGAAAAAT GGTGGATCAG AAATAGTTGT CACCAATAAG AACAAAAAGG 
     4261 AATATATTTA TCTTGTAATA CAATGGCGAT TTGTAAACCG AATCCAGAAG CAAATGGCTG 
     4321 CTTTTAAAGA GGGATTCTTT GAACTAATAC CACAGGATCT CATCAAAATT TTTGATGAAA 
     4381 ATGAACTAGA GCTTCTTATG TGTGGACTGG GAGATGTTGA TGTGAATGAC TGGAGGGAAC 
     4441 ATACAAAGTA TAAAAATGGC TACAGTGCAA ATCATCAGGT TATACAGTGG TTTTGGAAGG 
     4501 CTGTTTTAAT GATGGATTCA GAAAAAAGAA TAAGATTACT TCAGTTTGTC ACTGGCACAT 
     4561 CTCGGGTGCC TATGAATGGA TTTGCTGAAC TATACGGTTC AAATGGACCA CAGTCATTTA 
     4621 CAGTTGAACA GTGGGGTACT CCTGAAAAGC TGCCAAGAGC TCATACCTGT TTTAATCGCC 
     4681 TGGACTTGCC ACCTTATGAA TCATTTGAAG AATTATGGGA TAAACTTCAG ATGGCAATTG 
     4741 AAAACACCCA GGGCTTTGAT GGAGTTGATA CGCGTACGCG GCCGCTCGAG CAGAAACTCA 
     4801 TCTCAGAAGA GGATCTGGCA GCAAATGATA TCCTGGATTA CAAGGATGAC GACGATAAGG 
     4861 TTTAAACGGC CGGCCGCGGT CATAGCTGTT TCCTGAACAG ATCCCGGGTG GCATCCCTGT 
     4921 GACCCCTCCC CAGTGCCTCT CCTGGCCCTG GAAGTTGCCA CTCCAGTGCC CACCAGCCTT 
     4981 GTCCTAATAA AATTAAGTTG CATCATTTTG TCTGACTAGG TGTCCTTCTA TAATATTATG 
     5041 GGGTGGAGGG GGGTGGTATG GAGCAAGGGG CAAGTTGGGA AGACAACCTG TAGGGCCTGC 
     5101 GGGGTCTATT GGGAACCAAG CTGGAGTGCA GTGGCACAAT CTTGGCTCAC TGCAATCTCC 
     5161 GCCTCCTGGG TTCAAGCGAT TCTCCTGCCT CAGCCTCCCG AGTTGTTGGG ATTCCAGGCA 
     5221 TGCATGACCA GGCTCAGCTA ATTTTTGTTT TTTTGGTAGA GACGGGGTTT CACCATATTG 
     5281 GCCAGGCTGG TCTCCAACTC CTAATCTCAG GTGATCTACC CACCTTGGCC TCCCAAATTG 
     5341 CTGGGATTAC AGGCGTGAAC CACTGCTCCC TTCCCTGTCC TTCTGATTTT AAAATAACTA 
     5401 TACCAGCAGG AGGACGTCCA GACACAGCAT AGGCTACCTG GCCATGCCCA ACCGGTGGGA 
     5461 CATTTGAGTT GCTTGCTTGG CACTGTCCTC TCATGCGTTG GGTCCACTCA GTAGATGCCT 
     5521 GTTGAATTGG GTACGCGGCC AGCGGCGAGC GGTATCAGCT CACTCAAAGG CGGTAATACG 
     5581 GTTATCCACA GAATCAGGGG ATAACGCAGG AAAGAACATG TGAGCAAAAG GCCAGCAAAA 
     5641 GGCCAGGAAC CGTAAAAAGG CCGCGTTGCT GGCGTTTTTC CATAGGCTCC GCCCCCCTGA 
     5701 CGAGCATCAC AAAAATCGAC GCTCAAGTCA GAGGTGGCGA AACCCGACAG GACTATAAAG 
     5761 ATACCAGGCG TTTCCCCCTG GAAGCTCCCT CGTGCGCTCT CCTGTTCCGA CCCTGCCGCT 
     5821 TACCGGATAC CTGTCCGCCT TTCTCCCTTC GGGAAGCGTG GCGCTTTCTC ATAGCTCACG 
     5881 CTGTAGGTAT CTCAGTTCGG TGTAGGTCGT TCGCTCCAAG CTGGGCTGTG TGCACGAACC 
     5941 CCCCGTTCAG CCCGACCGCT GCGCCTTATC CGGTAACTAT CGTCTTGAGT CCAACCCGGT 
     6001 AAGACACGAC TTATCGCCAC TGGCAGCAGC CACTGGTAAC AGGATTAGCA GAGCGAGGTA 
     6061 TGTAGGCGGT GCTACAGAGT TCTTGAAGTG GTGGCCTAAC TACGGCTACA CTAGAAGAAC 
     6121 AGTATTTGGT ATCTGCGCTC TGCTGAAGCC AGTTACCTTC GGAAAAAGAG TTGGTAGCTC 
     6181 TTGATCCGGC AAACAAACCA CCGCTGGTAG CGGTGGTTTT TTTGTTTGCA AGCAGCAGAT 
     6241 TACGCGCAGA AAAAAAGGAT CTCAAGAAGA TCCTTTGATC TTTTCTACGG GGTCTGACGC 
     6301 TCAGTGGAAC GAAAACTCAC GTTAAGGGAT TTTGGTCATG AGATTATCAA AAAGGATCTT 
     6361 CACCTAGATC CTTTTAAATT AAAAATGAAG TTTTAAATCA ATCTAAAGTA TATATGAGTA 
     6421 ACCTGAGGCT ATGGCAGGGC CTGCCGCCCC GACGTTGGCT GCGAGCCCTG GGCCTTCACC 
     6481 CGAACTTGGG GGGTGGGGTG GGGAAAAGGA AGAAACGCGG GCGTATTGGC CCCAATGGGG 
     6541 TCTCGGTGGG GTATCGACAG AGTGCCAGCC CTGGGACCGA ACCCCGCGTT TATGAACAAA 
     6601 CGACCCAACA CCGTGCGTTT TATTCTGTCT TTTTATTGCC GTCATAGCGC GGGTTCCTTC 
     6661 CGGTATTGTC TCCTTCCGTG TTTCAGTTAG CCTCCCCCTA GGGTGGGCGA AGAACTCCAG 
     6721 CATGAGATCC CCGCGCTGGA GGATCATCCA GCCGGCGTCC CGGAAAACGA TTCCGAAGCC 
     6781 CAACCTTTCA TAGAAGGCGG CGGTGGAATC GAAATCTCGT GATGGCAGGT TGGGCGTCGC 
     6841 TTGGTCGGTC ATTTCGAACC CCAGAGTCCC GCTCAGAAGA ACTCGTCAAG AAGGCGATAG 
     6901 AAGGCGATGC GCTGCGAATC GGGAGCGGCG ATACCGTAAA GCACGAGGAA GCGGTCAGCC 
     6961 CATTCGCCGC CAAGCTCTTC AGCAATATCA CGGGTAGCCA ACGCTATGTC CTGATAGCGA 
     7021 TCCGCCACAC CCAGCCGGCC ACAGTCGATG AATCCAGAAA AGCGGCCATT TTCCACCATG 
     7081 ATATTCGGCA AGCAGGCATC GCCATGGGTC ACGACGAGAT CCTCGCCGTC GGGCATGCTC 
     7141 GCCTTGAGCC TGGCGAACAG TTCGGCTGGC GCGAGCCCCT GATGCTCTTC GTCCAGATCA 
     7201 TCCTGATCGA CAAGACCGGC TTCCATCCGA GTACGTGCTC GCTCGATGCG ATGTTTCGCT 
     7261 TGGTGGTCGA ATGGGCAGGT AGCCGGATCA AGCGTATGCA GCCGCCGCAT TGCATCAGCC 
     7321 ATGATGGATA CTTTCTCGGC AGGAGCAAGG TGAGATGACA GGAGATCCTG CCCCGGCACT 
     7381 TCGCCCAATA GCAGCCAGTC CCTTCCCGCT TCAGTGACAA CGTCGAGCAC AGCTGCGCAA 
     7441 GGAACGCCCG TCGTGGCCAG CCACGATAGC CGCGCTGCCT CGTCTTGCAG TTCATTCAGG 
     7501 GCACCGGACA GGTCGGTCTT GACAAAAAGA ACCGGGCGCC CCTGCGCTGA CAGCCGGAAC 
     7561 ACGGCGGCAT CAGAGCAGCC GATTGTCTGT TGTGCCCAGT CATAGCCGAA TAGCCTCTCC 
     7621 ACCCAAGCGG CCGGAGAACC TGCGTGCAAT CCATCTTGTT CAATCATGCG AAACGATCCT 
     7681 CATCCTGTCT CTTGATCGAT CTTTGCAAAA GCCTAGGCCT CCAAAAAAGC CTCCTCACTA 
     7741 CTTCTGGAAT AGCTCAGAGG CCGAGGCGGC CTCGGCCTCT GCATAAATAA AAAAAATTAG 
     7801 TCAGCCATGG GGCGGAGAAT GGGCGGAACT GGGCGGAGTT AGGGGCGGGA TGGGCGGAGT 
     7861 TAGGGGCGGG ACTATGGTTG CTGACTAATT GAGATGCATG CTTTGCATAC TTCTGCCTGC 
     7921 TGGGGAGCCT GGGGACTTTC CACACCTGGT TGCTGACTAA TTGAGATGCA TGCTTTGCAT 
     7981 ACTTCTGCCT GCTGGGGAGC CTGGGGACTT TCCACACCCT AACTGACACA CATTCCACAG 
     8041 CTGGTTCTTT CCGCCTCAGG ACTCTTCCTT TTTCAATATT ATTGAAGCAT TTATCAGGGT 
     8101 TATTGTCTCA TGAGCGGATA CATATTTGAA TGTATTTAGA AAAATAAACA AATAGGGGTT 
     8161 CCGCGCACAT TTCCCCGAAA AGTGCCACCT GACGCGCCCT GTAGCGGCGC ATTAAGCGCG 
     8221 GCGGGTGTGG TGGTTACGCG CAGCGTGACC GCTACACTTG CCAGCGCCCT AGCGCCCGCT 
     8281 CCTTTCGCTT TCTTCCCTTC CTTTCTCGCC ACGTTCGCCG GCTTTCCCCG TCAAGCTCTA 
     8341 AATCGGGGGC TCCCTTTAGG GTTCCGATTT AGTGCTTTAC GGCACCTCGA CCCCAAAAAA 
     8401 CTTGATTAGG GTGATGGTTC ACGTAGTGGG CCATCGCCCT GATAGACGGT TTTTCGCCCT 
     8461 TTGACGTTGG AGTCCACGTT CTTTAATAGT GGACTCTTGT TCCAAACTGG AACAACACTC 
     8521 AACCCTATCT CGGTCTATTC TTTTGATTTA TAAGGGATTT TGCCGATTTC GGCCTATTGG 
     8581 TTAAAAAATG AGCTGATTTA ACAAAAATTT AACGCGAATT TT
//