LOCUS       RC212731                8556 bp    DNA     circular  SYN 
DEFINITION  DMD (Myc-DDK-tagged)-Human dystrophin (DMD), transcript variant Dp140
ACCESSION   NM_004013
VERSION     NM_004013.2
SOURCE      synthetic construct 
FEATURES    Location/Qualifiers 
     misc_feature    201..924 
                     /label="CMV promoter"  
     misc_feature    839..856 
                     /label="Restriction Enzyme"  
     misc_feature    953..971 
                     /label="T7 promoter"  
     misc_feature    4713..4804 
                     /label="Myc-DDK"  
     misc_feature    4834..5419 
                     /label="PolyA signal"  
     misc_feature    4891..4910 
                     /label="Restriction Enzyme"  
     misc_feature    5695..6367 
                     /label="ColE1"  
     misc_feature    6797..7591 
                     /label="Kan/Neo"  
     misc_feature    7626..7928 
                     /label="SV40 ori"  
     misc_feature    8014..8491 
                     /label="f1 ori"  
     misc_feature    1020..1028 
                     /label="Restriction Enzyme"  
     misc_feature    4695..4701 
                     /label="Restriction Enzyme"  
     misc_feature    1020..4695 
                     /label="DMD"  
ORIGIN                      
        1 AACAAAATAT TAACGCTTAC AATTTCCATT CGCCATTCAG GCTGCGCAAC TGTTGGGAAG 
       61 GGCGATCGGT GCGGGCCTCT TCGCTATTAC GCCAGCTGGC GAAAGGGGGA TGTGCTGCAA 
      121 GGCGATTAAG TTGGGTAACG CCAGGGTTTT CCCAGTCACG ACGTTGTAAA ACGACGGCCA 
      181 GTGCCAAGCT GATCTATACA TTGAATCAAT ATTGGCAATT AGCCATATTA GTCATTGGTT 
      241 ATATAGCATA AATCAATATT GGCTATTGGC CATTGCATAC GTTGTATCTA TATCATAATA 
      301 TGTACATTTA TATTGGCTCA TGTCCAATAT GACCGCCATG TTGACATTGA TTATTGACTA 
      361 GTTATTAATA GTAATCAATT ACGGGGTCAT TAGTTCATAG CCCATATATG GAGTTCCGCG 
      421 TTACATAACT TACGGTAAAT GGCCCGCCTG GCTGACCGCC CAACGACCCC CGCCCATTGA 
      481 CGTCAATAAT GACGTATGTT CCCATAGTAA CGCCAATAGG GACTTTCCAT TGACGTCAAT 
      541 GGGTGGAGTA TTTACGGTAA ACTGCCCACT TGGCAGTACA TCAAGTGTAT CATATGCCAA 
      601 GTCCGCCCCC TATTGACGTC AATGACGGTA AATGGCCCGC CTGGCATTAT GCCCAGTACA 
      661 TGACCTTACG GGACTTTCCT ACTTGGCAGT ACATCTACGT ATTAGTCATC GCTATTACCA 
      721 TGGTGATGCG GTTTTGGCAG TACACCAATG GGCGTGGATA GCGGTTTGAC TCACGGGGAT 
      781 TTCCAAGTCT CCACCCCATT GACGTCAATG GGAGTTTGTT TTGGCACCAA AATCAACGGG 
      841 ACTTTCCAAA ATGTCGTAAT AACCCCGCCC CGTTGACGCA AATGGGCGGT AGGCGTGTAC 
      901 GGTGGGAGGT CTATATAAGC AGAGCTCGTT TAGTGAACCG TCAGAATTTT GTAATACGAC 
      961 TCACTATAGG GCGGCCGGGA ATTCGTCGAC TGGATCCGGT ACCGAGGAGA TCTGCCGCCG 
     1021 CGATCGCCAT GCCATCTTCC TTGATGTTGG AGGTACCTGC TCTGGCAGAT TTCAACCGGG 
     1081 CTTGGACAGA ACTTACCGAC TGGCTTTCTC TGCTTGATCA AGTTATAAAA TCACAGAGGG 
     1141 TGATGGTGGG TGACCTTGAG GATATCAACG AGATGATCAT CAAGCAGAAG GCAACAATGC 
     1201 AGGATTTGGA ACAGAGGCGT CCCCAGTTGG AAGAACTCAT TACCGCTGCC CAAAATTTGA 
     1261 AAAACAAGAC CAGCAATCAA GAGGCTAGAA CAATCATTAC GGATCGAATT GAAAGAATTC 
     1321 AGAATCAGTG GGATGAAGTA CAAGAACACC TTCAGAACCG GAGGCAACAG TTGAATGAAA 
     1381 TGTTAAAGGA TTCAACACAA TGGCTGGAAG CTAAGGAAGA AGCTGAGCAG GTCTTAGGAC 
     1441 AGGCCAGAGC CAAGCTTGAG TCATGGAAGG AGGGTCCCTA TACAGTAGAT GCAATCCAAA 
     1501 AGAAAATCAC AGAAACCAAG CAGTTGGCCA AAGACCTCCG CCAGTGGCAG ACAAATGTAG 
     1561 ATGTGGCAAA TGACTTGGCC CTGAAACTTC TCCGGGATTA TTCTGCAGAT GATACCAGAA 
     1621 AAGTCCACAT GATAACAGAG AATATCAATG CCTCTTGGAG AAGCATTCAT AAAAGGGTGA 
     1681 GTGAGCGAGA GGCTGCTTTG GAAGAAACTC ATAGATTACT GCAACAGTTC CCCCTGGACC 
     1741 TGGAAAAGTT TCTTGCCTGG CTTACAGAAG CTGAAACAAC TGCCAATGTC CTACAGGATG 
     1801 CTACCCGTAA GGAAAGGCTC CTAGAAGACT CCAAGGGAGT AAAAGAGCTG ATGAAACAAT 
     1861 GGCAAGACCT CCAAGGTGAA ATTGAAGCTC ACACAGATGT TTATCACAAC CTGGATGAAA 
     1921 ACAGCCAAAA AATCCTGAGA TCCCTGGAAG GTTCCGATGA TGCAGTCCTG TTACAAAGAC 
     1981 GTTTGGATAA CATGAACTTC AAGTGGAGTG AACTTCGGAA AAAGTCTCTC AACATTAGGT 
     2041 CCCATTTGGA AGCCAGTTCT GACCAGTGGA AGCGTCTGCA CCTTTCTCTG CAGGAACTTC 
     2101 TGGTGTGGCT ACAGCTGAAA GATGATGAAT TAAGCCGGCA GGCACCTATT GGAGGCGACT 
     2161 TTCCAGCAGT TCAGAAGCAG AACGATGTAC ATAGGGCCTT CAAGAGGGAA TTGAAAACTA 
     2221 AAGAACCTGT AATCATGAGT ACTCTTGAGA CTGTACGAAT ATTTCTGACA GAGCAGCCTT 
     2281 TGGAAGGACT AGAGAAACTC TACCAGGAGC CCAGAGAGCT GCCTCCTGAG GAGAGAGCCC 
     2341 AGAATGTCAC TCGGCTTCTA CGAAAGCAGG CTGAGGAGGT CAATACTGAG TGGGAAAAAT 
     2401 TGAACCTGCA CTCCGCTGAC TGGCAGAGAA AAATAGATGA GACCCTTGAA AGACTCCAGG 
     2461 AACTTCAAGA GGCCACGGAT GAGCTGGACC TCAAGCTGCG CCAAGCTGAG GTGATCAAGG 
     2521 GATCCTGGCA GCCCGTGGGC GATCTCCTCA TTGACTCTCT CCAAGATCAC CTCGAGAAAG 
     2581 TCAAGGCACT TCGAGGAGAA ATTGCGCCTC TGAAAGAGAA CGTGAGCCAC GTCAATGACC 
     2641 TTGCTCGCCA GCTTACCACT TTGGGCATTC AGCTCTCACC GTATAACCTC AGCACTCTGG 
     2701 AAGACCTGAA CACCAGATGG AAGCTTCTGC AGGTGGCCGT CGAGGACCGA GTCAGGCAGC 
     2761 TGCATGAAGC CCACAGGGAC TTTGGTCCAG CATCTCAGCA CTTTCTTTCC ACGTCTGTCC 
     2821 AGGGTCCCTG GGAGAGAGCC ATCTCGCCAA ACAAAGTGCC CTACTATATC AACCACGAGA 
     2881 CTCAAACAAC TTGCTGGGAC CATCCCAAAA TGACAGAGCT CTACCAGTCT TTAGCTGACC 
     2941 TGAATAATGT CAGATTCTCA GCTTATAGGA CTGCCATGAA ACTCCGAAGA CTGCAGAAGG 
     3001 CCCTTTGCTT GGATCTCTTG AGCCTGTCAG CTGCATGTGA TGCCTTGGAC CAGCACAACC 
     3061 TCAAGCAAAA TGACCAGCCC ATGGATATCC TGCAGATTAT TAATTGTTTG ACCACTATTT 
     3121 ATGACCGCCT GGAGCAAGAG CACAACAATT TGGTCAACGT CCCTCTCTGC GTGGATATGT 
     3181 GTCTGAACTG GCTGCTGAAT GTTTATGATA CGGGACGAAC AGGGAGGATC CGTGTCCTGT 
     3241 CTTTTAAAAC TGGCATCATT TCCCTGTGTA AAGCACATTT GGAAGACAAG TACAGATACC 
     3301 TTTTCAAGCA AGTGGCAAGT TCAACAGGAT TTTGTGACCA GCGCAGGCTG GGCCTCCTTC 
     3361 TGCATGATTC TATCCAAATT CCAAGACAGT TGGGTGAAGT TGCATCCTTT GGGGGCAGTA 
     3421 ACATTGAGCC AAGTGTCCGG AGCTGCTTCC AATTTGCTAA TAATAAGCCA GAGATCGAAG 
     3481 CGGCCCTCTT CCTAGACTGG ATGAGACTGG AACCCCAGTC CATGGTGTGG CTGCCCGTCC 
     3541 TGCACAGAGT GGCTGCTGCA GAAACTGCCA AGCATCAGGC CAAATGTAAC ATCTGCAAAG 
     3601 AGTGTCCAAT CATTGGATTC AGGTACAGGA GTCTAAAGCA CTTTAATTAT GACATCTGCC 
     3661 AAAGCTGCTT TTTTTCTGGT CGAGTTGCAA AAGGCCATAA AATGCACTAT CCCATGGTGG 
     3721 AATATTGCAC TCCGACTACA TCAGGAGAAG ATGTTCGAGA CTTTGCCAAG GTACTAAAAA 
     3781 ACAAATTTCG AACCAAAAGG TATTTTGCGA AGCATCCCCG AATGGGCTAC CTGCCAGTGC 
     3841 AGACTGTCTT AGAGGGGGAC AACATGGAAA CTCCCGTTAC TCTGATCAAC TTCTGGCCAG 
     3901 TAGATTCTGC GCCTGCCTCG TCCCCTCAGC TTTCACACGA TGATACTCAT TCACGCATTG 
     3961 AACATTATGC TAGCAGGCTA GCAGAAATGG AAAACAGCAA TGGATCTTAT CTAAATGATA 
     4021 GCATCTCTCC TAATGAGAGC ATAGATGATG AACATTTGTT AATCCAGCAT TACTGCCAAA 
     4081 GTTTGAACCA GGACTCCCCC CTGAGCCAGC CTCGTAGTCC TGCCCAGATC TTGATTTCCT 
     4141 TAGAGAGTGA GGAAAGAGGG GAGCTAGAGA GAATCCTAGC AGATCTTGAG GAAGAAAACA 
     4201 GGAATCTGCA AGCAGAATAT GACCGTCTAA AGCAGCAGCA CGAACATAAA GGCCTGTCCC 
     4261 CACTGCCGTC CCCTCCTGAA ATGATGCCCA CCTCTCCCCA GAGTCCCCGG GATGCTGAGC 
     4321 TCATTGCTGA GGCCAAGCTA CTGCGTCAAC ACAAAGGCCG CCTGGAAGCC AGGATGCAAA 
     4381 TCCTGGAAGA CCACAATAAA CAGCTGGAGT CACAGTTACA CAGGCTAAGG CAGCTGCTGG 
     4441 AGCAACCCCA GGCAGAGGCC AAAGTGAATG GCACAACGGT GTCCTCTCCT TCTACCTCTC 
     4501 TACAGAGGTC CGACAGCAGT CAGCCTATGC TGCTCCGAGT GGTTGGCAGT CAAACTTCGG 
     4561 ACTCCATGGG TGAGGAAGAT CTTCTCAGTC CTCCCCAGGA CACAAGCACA GGGTTAGAGG 
     4621 AGGTGATGGA GCAACTCAAC AACTCCTTCC CTAGTTCAAG AGGAAGAAAT ACCCCTGGAA 
     4681 AGCCAATGAG AGAGGACACA ATGACGCGTA CGCGGCCGCT CGAGCAGAAA CTCATCTCAG 
     4741 AAGAGGATCT GGCAGCAAAT GATATCCTGG ATTACAAGGA TGACGACGAT AAGGTTTAAA 
     4801 CGGCCGGCCG CGGTCATAGC TGTTTCCTGA ACAGATCCCG GGTGGCATCC CTGTGACCCC 
     4861 TCCCCAGTGC CTCTCCTGGC CCTGGAAGTT GCCACTCCAG TGCCCACCAG CCTTGTCCTA 
     4921 ATAAAATTAA GTTGCATCAT TTTGTCTGAC TAGGTGTCCT TCTATAATAT TATGGGGTGG 
     4981 AGGGGGGTGG TATGGAGCAA GGGGCAAGTT GGGAAGACAA CCTGTAGGGC CTGCGGGGTC 
     5041 TATTGGGAAC CAAGCTGGAG TGCAGTGGCA CAATCTTGGC TCACTGCAAT CTCCGCCTCC 
     5101 TGGGTTCAAG CGATTCTCCT GCCTCAGCCT CCCGAGTTGT TGGGATTCCA GGCATGCATG 
     5161 ACCAGGCTCA GCTAATTTTT GTTTTTTTGG TAGAGACGGG GTTTCACCAT ATTGGCCAGG 
     5221 CTGGTCTCCA ACTCCTAATC TCAGGTGATC TACCCACCTT GGCCTCCCAA ATTGCTGGGA 
     5281 TTACAGGCGT GAACCACTGC TCCCTTCCCT GTCCTTCTGA TTTTAAAATA ACTATACCAG 
     5341 CAGGAGGACG TCCAGACACA GCATAGGCTA CCTGGCCATG CCCAACCGGT GGGACATTTG 
     5401 AGTTGCTTGC TTGGCACTGT CCTCTCATGC GTTGGGTCCA CTCAGTAGAT GCCTGTTGAA 
     5461 TTGGGTACGC GGCCAGCGGC GAGCGGTATC AGCTCACTCA AAGGCGGTAA TACGGTTATC 
     5521 CACAGAATCA GGGGATAACG CAGGAAAGAA CATGTGAGCA AAAGGCCAGC AAAAGGCCAG 
     5581 GAACCGTAAA AAGGCCGCGT TGCTGGCGTT TTTCCATAGG CTCCGCCCCC CTGACGAGCA 
     5641 TCACAAAAAT CGACGCTCAA GTCAGAGGTG GCGAAACCCG ACAGGACTAT AAAGATACCA 
     5701 GGCGTTTCCC CCTGGAAGCT CCCTCGTGCG CTCTCCTGTT CCGACCCTGC CGCTTACCGG 
     5761 ATACCTGTCC GCCTTTCTCC CTTCGGGAAG CGTGGCGCTT TCTCATAGCT CACGCTGTAG 
     5821 GTATCTCAGT TCGGTGTAGG TCGTTCGCTC CAAGCTGGGC TGTGTGCACG AACCCCCCGT 
     5881 TCAGCCCGAC CGCTGCGCCT TATCCGGTAA CTATCGTCTT GAGTCCAACC CGGTAAGACA 
     5941 CGACTTATCG CCACTGGCAG CAGCCACTGG TAACAGGATT AGCAGAGCGA GGTATGTAGG 
     6001 CGGTGCTACA GAGTTCTTGA AGTGGTGGCC TAACTACGGC TACACTAGAA GAACAGTATT 
     6061 TGGTATCTGC GCTCTGCTGA AGCCAGTTAC CTTCGGAAAA AGAGTTGGTA GCTCTTGATC 
     6121 CGGCAAACAA ACCACCGCTG GTAGCGGTGG TTTTTTTGTT TGCAAGCAGC AGATTACGCG 
     6181 CAGAAAAAAA GGATCTCAAG AAGATCCTTT GATCTTTTCT ACGGGGTCTG ACGCTCAGTG 
     6241 GAACGAAAAC TCACGTTAAG GGATTTTGGT CATGAGATTA TCAAAAAGGA TCTTCACCTA 
     6301 GATCCTTTTA AATTAAAAAT GAAGTTTTAA ATCAATCTAA AGTATATATG AGTAACCTGA 
     6361 GGCTATGGCA GGGCCTGCCG CCCCGACGTT GGCTGCGAGC CCTGGGCCTT CACCCGAACT 
     6421 TGGGGGGTGG GGTGGGGAAA AGGAAGAAAC GCGGGCGTAT TGGCCCCAAT GGGGTCTCGG 
     6481 TGGGGTATCG ACAGAGTGCC AGCCCTGGGA CCGAACCCCG CGTTTATGAA CAAACGACCC 
     6541 AACACCGTGC GTTTTATTCT GTCTTTTTAT TGCCGTCATA GCGCGGGTTC CTTCCGGTAT 
     6601 TGTCTCCTTC CGTGTTTCAG TTAGCCTCCC CCTAGGGTGG GCGAAGAACT CCAGCATGAG 
     6661 ATCCCCGCGC TGGAGGATCA TCCAGCCGGC GTCCCGGAAA ACGATTCCGA AGCCCAACCT 
     6721 TTCATAGAAG GCGGCGGTGG AATCGAAATC TCGTGATGGC AGGTTGGGCG TCGCTTGGTC 
     6781 GGTCATTTCG AACCCCAGAG TCCCGCTCAG AAGAACTCGT CAAGAAGGCG ATAGAAGGCG 
     6841 ATGCGCTGCG AATCGGGAGC GGCGATACCG TAAAGCACGA GGAAGCGGTC AGCCCATTCG 
     6901 CCGCCAAGCT CTTCAGCAAT ATCACGGGTA GCCAACGCTA TGTCCTGATA GCGATCCGCC 
     6961 ACACCCAGCC GGCCACAGTC GATGAATCCA GAAAAGCGGC CATTTTCCAC CATGATATTC 
     7021 GGCAAGCAGG CATCGCCATG GGTCACGACG AGATCCTCGC CGTCGGGCAT GCTCGCCTTG 
     7081 AGCCTGGCGA ACAGTTCGGC TGGCGCGAGC CCCTGATGCT CTTCGTCCAG ATCATCCTGA 
     7141 TCGACAAGAC CGGCTTCCAT CCGAGTACGT GCTCGCTCGA TGCGATGTTT CGCTTGGTGG 
     7201 TCGAATGGGC AGGTAGCCGG ATCAAGCGTA TGCAGCCGCC GCATTGCATC AGCCATGATG 
     7261 GATACTTTCT CGGCAGGAGC AAGGTGAGAT GACAGGAGAT CCTGCCCCGG CACTTCGCCC 
     7321 AATAGCAGCC AGTCCCTTCC CGCTTCAGTG ACAACGTCGA GCACAGCTGC GCAAGGAACG 
     7381 CCCGTCGTGG CCAGCCACGA TAGCCGCGCT GCCTCGTCTT GCAGTTCATT CAGGGCACCG 
     7441 GACAGGTCGG TCTTGACAAA AAGAACCGGG CGCCCCTGCG CTGACAGCCG GAACACGGCG 
     7501 GCATCAGAGC AGCCGATTGT CTGTTGTGCC CAGTCATAGC CGAATAGCCT CTCCACCCAA 
     7561 GCGGCCGGAG AACCTGCGTG CAATCCATCT TGTTCAATCA TGCGAAACGA TCCTCATCCT 
     7621 GTCTCTTGAT CGATCTTTGC AAAAGCCTAG GCCTCCAAAA AAGCCTCCTC ACTACTTCTG 
     7681 GAATAGCTCA GAGGCCGAGG CGGCCTCGGC CTCTGCATAA ATAAAAAAAA TTAGTCAGCC 
     7741 ATGGGGCGGA GAATGGGCGG AACTGGGCGG AGTTAGGGGC GGGATGGGCG GAGTTAGGGG 
     7801 CGGGACTATG GTTGCTGACT AATTGAGATG CATGCTTTGC ATACTTCTGC CTGCTGGGGA 
     7861 GCCTGGGGAC TTTCCACACC TGGTTGCTGA CTAATTGAGA TGCATGCTTT GCATACTTCT 
     7921 GCCTGCTGGG GAGCCTGGGG ACTTTCCACA CCCTAACTGA CACACATTCC ACAGCTGGTT 
     7981 CTTTCCGCCT CAGGACTCTT CCTTTTTCAA TATTATTGAA GCATTTATCA GGGTTATTGT 
     8041 CTCATGAGCG GATACATATT TGAATGTATT TAGAAAAATA AACAAATAGG GGTTCCGCGC 
     8101 ACATTTCCCC GAAAAGTGCC ACCTGACGCG CCCTGTAGCG GCGCATTAAG CGCGGCGGGT 
     8161 GTGGTGGTTA CGCGCAGCGT GACCGCTACA CTTGCCAGCG CCCTAGCGCC CGCTCCTTTC 
     8221 GCTTTCTTCC CTTCCTTTCT CGCCACGTTC GCCGGCTTTC CCCGTCAAGC TCTAAATCGG 
     8281 GGGCTCCCTT TAGGGTTCCG ATTTAGTGCT TTACGGCACC TCGACCCCAA AAAACTTGAT 
     8341 TAGGGTGATG GTTCACGTAG TGGGCCATCG CCCTGATAGA CGGTTTTTCG CCCTTTGACG 
     8401 TTGGAGTCCA CGTTCTTTAA TAGTGGACTC TTGTTCCAAA CTGGAACAAC ACTCAACCCT 
     8461 ATCTCGGTCT ATTCTTTTGA TTTATAAGGG ATTTTGCCGA TTTCGGCCTA TTGGTTAAAA 
     8521 AATGAGCTGA TTTAACAAAA ATTTAACGCG AATTTT
//