LOCUS       RC212712                9267 bp    DNA     circular  SYN 
DEFINITION  MAGI1 (Myc-DDK-tagged)-Human membrane associated guanylate kinase, WW and PDZ domain containing 1 (MAGI1), transcript variant 3
ACCESSION   NM_001033057
VERSION     NM_001033057.1
SOURCE      synthetic construct 
FEATURES    Location/Qualifiers 
     misc_feature    201..924 
                     /label="CMV promoter"  
     misc_feature    839..856 
                     /label="Restriction Enzyme"  
     misc_feature    953..971 
                     /label="T7 promoter"  
     misc_feature    5424..5515 
                     /label="Myc-DDK"  
     misc_feature    5545..6130 
                     /label="PolyA signal"  
     misc_feature    5602..5621 
                     /label="Restriction Enzyme"  
     misc_feature    6406..7078 
                     /label="ColE1"  
     misc_feature    7508..8302 
                     /label="Kan/Neo"  
     misc_feature    8337..8639 
                     /label="SV40 ori"  
     misc_feature    8725..9202 
                     /label="f1 ori"  
     misc_feature    1020..1028 
                     /label="Restriction Enzyme"  
     misc_feature    5406..5412 
                     /label="Restriction Enzyme"  
     misc_feature    1020..5406 
                     /label="MAGI1"  
ORIGIN                      
        1 AACAAAATAT TAACGCTTAC AATTTCCATT CGCCATTCAG GCTGCGCAAC TGTTGGGAAG 
       61 GGCGATCGGT GCGGGCCTCT TCGCTATTAC GCCAGCTGGC GAAAGGGGGA TGTGCTGCAA 
      121 GGCGATTAAG TTGGGTAACG CCAGGGTTTT CCCAGTCACG ACGTTGTAAA ACGACGGCCA 
      181 GTGCCAAGCT GATCTATACA TTGAATCAAT ATTGGCAATT AGCCATATTA GTCATTGGTT 
      241 ATATAGCATA AATCAATATT GGCTATTGGC CATTGCATAC GTTGTATCTA TATCATAATA 
      301 TGTACATTTA TATTGGCTCA TGTCCAATAT GACCGCCATG TTGACATTGA TTATTGACTA 
      361 GTTATTAATA GTAATCAATT ACGGGGTCAT TAGTTCATAG CCCATATATG GAGTTCCGCG 
      421 TTACATAACT TACGGTAAAT GGCCCGCCTG GCTGACCGCC CAACGACCCC CGCCCATTGA 
      481 CGTCAATAAT GACGTATGTT CCCATAGTAA CGCCAATAGG GACTTTCCAT TGACGTCAAT 
      541 GGGTGGAGTA TTTACGGTAA ACTGCCCACT TGGCAGTACA TCAAGTGTAT CATATGCCAA 
      601 GTCCGCCCCC TATTGACGTC AATGACGGTA AATGGCCCGC CTGGCATTAT GCCCAGTACA 
      661 TGACCTTACG GGACTTTCCT ACTTGGCAGT ACATCTACGT ATTAGTCATC GCTATTACCA 
      721 TGGTGATGCG GTTTTGGCAG TACACCAATG GGCGTGGATA GCGGTTTGAC TCACGGGGAT 
      781 TTCCAAGTCT CCACCCCATT GACGTCAATG GGAGTTTGTT TTGGCACCAA AATCAACGGG 
      841 ACTTTCCAAA ATGTCGTAAT AACCCCGCCC CGTTGACGCA AATGGGCGGT AGGCGTGTAC 
      901 GGTGGGAGGT CTATATAAGC AGAGCTCGTT TAGTGAACCG TCAGAATTTT GTAATACGAC 
      961 TCACTATAGG GCGGCCGGGA ATTCGTCGAC TGGATCCGGT ACCGAGGAGA TCTGCCGCCG 
     1021 CGATCGCCAT GTCCAAAGTG ATCCAGAAGA AGAACCACTG GACTAGCAGG GTTCACGAAT 
     1081 GCACCGTGAA GCGGGGACCC CAGGGCGAGC TGGGGGTGAC GGTGCTGGGA GGCGCGGAGC 
     1141 ACGGGGAGTT TCCGTACGTC GGAGCGGTGG CGGCGGTCGA GGCAGCGGGG CTTCCCGGCG 
     1201 GCGGCGAGGG CCCGAGGCTG GGCGAAGGGG AGCTGCTTCT GGAGGTGCAG GGGGTCCGGG 
     1261 TGTCCGGCTT GCCCCGCTAT GACGTGCTGG GGGTCATCGA CAGCTGCAAG GAGGCCGTCA 
     1321 CCTTCAAGGC CGTCAGACAA GGAGGAAGGC TGAACAAGGA CCTGCGACAT TTTCTCAATC 
     1381 AGCGATTCCA GAAGGGGTCT CCTGATCATG AGCTCCAGCA GACCATAAGG GATAACCTTT 
     1441 ACCGCCATGC TGTGCCTTGC ACAACCCGAT CTCCCAGAGA AGGAGAAGTG CCTGGCGTGG 
     1501 ACTATAACTT TCTGACTGTG AAGGAGTTCT TGGACCTCGA GCAGAGTGGG ACTCTTCTGG 
     1561 AAGTCGGCAC CTATGAAGGA AACTATTATG GGACACCCAA GCCTCCTAGC CAGCCAGTCA 
     1621 GTGGGAAAGT GATCACGACG GATGCCTTGC ACAGCCTTCA GTCTGGCTCT AAGCAGTCGA 
     1681 CCCCGAAGCG AACCAAGTCC TACAATGATA TGCAAAATGC TGGCATAGTC CACGCGGAGA 
     1741 ATGAGGAGGA GGATGACGTT CCTGAAATGA ACAGCAGCTT TACAGCCGAT TCTGGTGAAC 
     1801 AAGAGGAGCA CACTCTCCAA GAAACAGCAT TACCACCTGT GAATAGTAGC ATCATCGCTG 
     1861 CTCCCATCAC GGACCCTTCT CAGAAGTTCC CTCAATACCT ACCTCTTTCT GCAGAGGATA 
     1921 ATTTAGGTCC TCTACCTGAA AACTGGGAGA TGGCCTATAC TGAAAATGGA GAAGTCTATT 
     1981 TTATAGACCA TAACACGAAA ACAACATCTT GGTTAGACCC TCGGTGCCTA AACAAGCAGC 
     2041 AGAAGCCACT GGAAGAGTGT GAAGATGATG AAGGGGTACA CACCGAGGAG CTGGACAGTG 
     2101 AACTAGAACT GCCTGCTGGT TGGGAAAAGA TTGAAGACCC TGTCTATGGT ATCTACTATG 
     2161 TAGACCACAT CAACAGGAAG ACACAATATG AGAACCCGGT TCTAGAAGCC AAACGGAAGA 
     2221 AGCAGCTTGA GCAGCAGCAG CAGCAGCAAC AGCAGCAGCA ACAGCAGCAG CAGCAGCAGC 
     2281 AGCAGCAGCA GACAGAAGAA TGGACAGAAG ATCACTCAGC CCTTGTGCCT CCTGTTATTC 
     2341 CAAACCACCC TCCAAGCAAT CCAGAGCCAG CCAGAGAAGT TCCACTTCAG GGCAAACCCT 
     2401 TTTTTACACG AAACCCTTCT GAGTTGAAAG GCAAGTTCAT TCACACAAAG CTGCGGAAAA 
     2461 GCAGTCGTGG CTTTGGCTTC ACGGTGGTTG GAGGGGATGA ACCTGATGAG TTTCTCCAGA 
     2521 TCAAGAGCTT GGTCCTAGAT GGTCCTGCTG CATTGGATGG CAAGATGGAA ACAGGGGATG 
     2581 TGATTGTAAG TGTGAATGAC ACCTGTGTTT TGGGACACAC ACATGCTCAA GTTGTGAAGA 
     2641 TCTTCCAGTC CATCCCCATT GGTGCCAGCG TGGACCTTGA ACTCTGCCGA GGTTATCCAT 
     2701 TGCCTTTTGA TCCAGATGAC CCCAATACAA GTTTAGTGAC CTCGGTAGCC ATTTTGGATA 
     2761 AAGAACCAAT TATTGTGAAT GGGCAAGAGA CCTATGATTC ACCAGCTAGC CACAGTAGTA 
     2821 AAACAGGCAA AGTCAATGGC ATGAAGGATG CCAGGCCAAG CAGCCCAGCG GACGTGGCTT 
     2881 CAAATAGTTC TCATGGTTAT CCTAATGACA CTGTTTCTTT GGCTTCCTCC ATAGCCACTC 
     2941 AGCCAGAACT CATAACTGTT CATATTGTCA AAGGGCCAAT GGGCTTTGGT TTTACTATCG 
     3001 CAGACAGTCC TGGTGGGGGT GGCCAAAGAG TGAAACAGAT TGTTGACAGC CCAAGGTGCC 
     3061 GAGGCCTGAA AGAAGGGGAT CTCATAGTGG AAGTTAATAA GAAGAACGTG CAGGCCCTAA 
     3121 CTCACAACCA AGTGGTGGAT ATGCTGGTTG AGTGTCCTAA GGGAAGTGAG GTCACATTGT 
     3181 TGGTGCAACG AGGAGGGCTG CCAGTTCCCA AGAAGAGCCC AAAGTCGCAA CCACTGGAAA 
     3241 GGAAAGACAG CCAGAATAGT TCTCAGCACA GTGTTTCCAG CCACCGAAGC CTGCACACAG 
     3301 CATCCCCAAG CCACAGCACA CAGGTGCTCC CCGAGTTCCC ACCTGCAGAG GCCCAAGCTC 
     3361 CAGATCAAAC TGACAGCTCT GGCCAGAAAA AACCAGATCC TTTTAAAATC TGGGCCCAGT 
     3421 CCAGGAGCAT GTATGAAAAC CGACTTCCAG ATTACCAGGA ACAGGACATC TTCCTCTGGA 
     3481 GAAAAGAGAC TGGATTTGGA TTTAGGATTC TGGGTGGAAA TGAACCAGGG GAACCTATTT 
     3541 ATATTGGTCA CATCGTACCA CTGGGTGCTG CTGATACTGA CGGCCGCCTG AGGTCTGGAG 
     3601 ATGAATTAAT CTGTGTGGAT GGGACGCCAG TAATTGGAAA ATCACACCAG CTTGTGGTCC 
     3661 AGCTTATGCA ACAAGCTGCC AAGCAAGGCC ACGTCAATCT CACGGTGCGG CGTAAAGTGG 
     3721 TTTTTGCGGT GCCCAAAACC GAGAACGAGG TGCCCTCGCC AGCCTCCTCT CATCACAGTA 
     3781 GCAACCAGCC GGCCTCGCTG ACAGAAGAGA AGCGCACTCC GCAGGGCAGC CAGAACTCGC 
     3841 TGAACACGGT GAGCTCGGGC AGCGGCAGCA CCAGCGGCAT CGGCAGTGGC GGCGGCGGGG 
     3901 GCAGCGGCGT GGTCAGCACC GTGGTGCAGC CCTACGACGT GGAGATCCGG CGCGGGGAGA 
     3961 ACGAGGGCTT CGGCTTCGTC ATCGTGTCCT CGGTGAGCAG GCCCGAAGCA GGCACGACCT 
     4021 TTGGCAATGC ATGTGTGGCT ATGCCTCACA AAATAGGTCG GATTATTGAG GGGAGCCCTG 
     4081 CTGACCGCTG TGGCAAGCTG AAAGTAGGAG ACCGGATCTT GGCAGTAAAT GGATGTTCCA 
     4141 TCACCAACAA ATCCCATTCA GACATTGTGA ACCTAATCAA GGAAGCGGGA AACACAGTTA 
     4201 CCCTCCGCAT CATTCCTGGG GATGAGTCCT CGAATGCCAC CTTGCTGACC AATGCAGAGA 
     4261 AGATTGCCAC CATCACCACC ACACACACCC CTTCTCAGCA AGGGACCCAG GAGACAAGGA 
     4321 ATACCACCAA ACCAAAGCAG GAATCTCAAT TTGAGTTCAA AGCACCCCAA GCAACACAGG 
     4381 AGCAAGATTT TTACACTGTG GAACTGGAAA GAGGAGCCAA GGGATTTGGC TTTAGCCTTC 
     4441 GAGGAGGCCG AGAGTATAAC ATGGACCTCT ATGTTCTGCG CTTAGCAGAG GACGGTCCTG 
     4501 CGGAGAGGTG TGGAAAGATG AGGATTGGTG ATGAAATTTT AGAGATCAAT GGTGAGACCA 
     4561 CCAAAAACAT GAAGCATTCT CGAGCTATAG AACTGATTAA GAATGGTGGC CGCAGAGTTC 
     4621 GTCTGTTTCT GAAGCGGGGA GACGGCTCAG TACCAGAATA TGACCCCAGC AGCGACCGCC 
     4681 ACGGCCCCGC CACCGGTCCA CAAGGTGTTC CGGAAGTGAG GGCCGGGCCC GACCGCCGGC 
     4741 AGCATCCGTC ATTGGAGTCC AGTTACCCAC CCGATCTTCA CAAATCATCA CCACATGGGG 
     4801 AAAAGCGGGC ACACGCAAGG GATCCGAAAG GCAGCAGAGA GTATAGCAGA CAACCCAATG 
     4861 AACACCACAC CTGGAATGGG ACTTCGAGGA AACCCGACAG CGGGGCATGC CGACCCAAGG 
     4921 ACCGGGCGCC GGAGGGACGG AGGGACGCGC AGGCCGAGCG CGCGGCAGCC GCCAACGGCC 
     4981 CCAAGAGGCG GTCCCCAGAG AAGCGGAGGG AGGGCACCCG CAGCGCCGAC AACACTTTGG 
     5041 AGAGGAGGGA GAAGCACGAG AAGAGACGAG ACGTCTCTCC GGAGCGGAGG CGAGAGCGGT 
     5101 CACCCACCCG CAGGAGAGAC GGCTCCCCCA GCCGGCGGAG ACGGTCTCTG GAGAGACTCC 
     5161 TGGAGCAGAG GAGGTCCCCC GAGCGCAGGA GAGGGGGCTC GCCCGAGCGC AGGGCCAAGT 
     5221 CCACCGACCG GAGGCGCGCA CGCTCCCCCG AGCGCAGGAG AGAGCGGTCC CTGGACAAAA 
     5281 GGAACAGAGA GGACAGAGCC AGCCACCGAG AAAGGGAAGA GGCGAATCTG AAACAGGATG 
     5341 CCGGCAGAAG TTCCAGACAT CCCCCGGAGC AGAGAAGGCG ACCTTACAAA GAATGTAGCA 
     5401 CCGACCTCAG TATCACGCGT ACGCGGCCGC TCGAGCAGAA ACTCATCTCA GAAGAGGATC 
     5461 TGGCAGCAAA TGATATCCTG GATTACAAGG ATGACGACGA TAAGGTTTAA ACGGCCGGCC 
     5521 GCGGTCATAG CTGTTTCCTG AACAGATCCC GGGTGGCATC CCTGTGACCC CTCCCCAGTG 
     5581 CCTCTCCTGG CCCTGGAAGT TGCCACTCCA GTGCCCACCA GCCTTGTCCT AATAAAATTA 
     5641 AGTTGCATCA TTTTGTCTGA CTAGGTGTCC TTCTATAATA TTATGGGGTG GAGGGGGGTG 
     5701 GTATGGAGCA AGGGGCAAGT TGGGAAGACA ACCTGTAGGG CCTGCGGGGT CTATTGGGAA 
     5761 CCAAGCTGGA GTGCAGTGGC ACAATCTTGG CTCACTGCAA TCTCCGCCTC CTGGGTTCAA 
     5821 GCGATTCTCC TGCCTCAGCC TCCCGAGTTG TTGGGATTCC AGGCATGCAT GACCAGGCTC 
     5881 AGCTAATTTT TGTTTTTTTG GTAGAGACGG GGTTTCACCA TATTGGCCAG GCTGGTCTCC 
     5941 AACTCCTAAT CTCAGGTGAT CTACCCACCT TGGCCTCCCA AATTGCTGGG ATTACAGGCG 
     6001 TGAACCACTG CTCCCTTCCC TGTCCTTCTG ATTTTAAAAT AACTATACCA GCAGGAGGAC 
     6061 GTCCAGACAC AGCATAGGCT ACCTGGCCAT GCCCAACCGG TGGGACATTT GAGTTGCTTG 
     6121 CTTGGCACTG TCCTCTCATG CGTTGGGTCC ACTCAGTAGA TGCCTGTTGA ATTGGGTACG 
     6181 CGGCCAGCGG CGAGCGGTAT CAGCTCACTC AAAGGCGGTA ATACGGTTAT CCACAGAATC 
     6241 AGGGGATAAC GCAGGAAAGA ACATGTGAGC AAAAGGCCAG CAAAAGGCCA GGAACCGTAA 
     6301 AAAGGCCGCG TTGCTGGCGT TTTTCCATAG GCTCCGCCCC CCTGACGAGC ATCACAAAAA 
     6361 TCGACGCTCA AGTCAGAGGT GGCGAAACCC GACAGGACTA TAAAGATACC AGGCGTTTCC 
     6421 CCCTGGAAGC TCCCTCGTGC GCTCTCCTGT TCCGACCCTG CCGCTTACCG GATACCTGTC 
     6481 CGCCTTTCTC CCTTCGGGAA GCGTGGCGCT TTCTCATAGC TCACGCTGTA GGTATCTCAG 
     6541 TTCGGTGTAG GTCGTTCGCT CCAAGCTGGG CTGTGTGCAC GAACCCCCCG TTCAGCCCGA 
     6601 CCGCTGCGCC TTATCCGGTA ACTATCGTCT TGAGTCCAAC CCGGTAAGAC ACGACTTATC 
     6661 GCCACTGGCA GCAGCCACTG GTAACAGGAT TAGCAGAGCG AGGTATGTAG GCGGTGCTAC 
     6721 AGAGTTCTTG AAGTGGTGGC CTAACTACGG CTACACTAGA AGAACAGTAT TTGGTATCTG 
     6781 CGCTCTGCTG AAGCCAGTTA CCTTCGGAAA AAGAGTTGGT AGCTCTTGAT CCGGCAAACA 
     6841 AACCACCGCT GGTAGCGGTG GTTTTTTTGT TTGCAAGCAG CAGATTACGC GCAGAAAAAA 
     6901 AGGATCTCAA GAAGATCCTT TGATCTTTTC TACGGGGTCT GACGCTCAGT GGAACGAAAA 
     6961 CTCACGTTAA GGGATTTTGG TCATGAGATT ATCAAAAAGG ATCTTCACCT AGATCCTTTT 
     7021 AAATTAAAAA TGAAGTTTTA AATCAATCTA AAGTATATAT GAGTAACCTG AGGCTATGGC 
     7081 AGGGCCTGCC GCCCCGACGT TGGCTGCGAG CCCTGGGCCT TCACCCGAAC TTGGGGGGTG 
     7141 GGGTGGGGAA AAGGAAGAAA CGCGGGCGTA TTGGCCCCAA TGGGGTCTCG GTGGGGTATC 
     7201 GACAGAGTGC CAGCCCTGGG ACCGAACCCC GCGTTTATGA ACAAACGACC CAACACCGTG 
     7261 CGTTTTATTC TGTCTTTTTA TTGCCGTCAT AGCGCGGGTT CCTTCCGGTA TTGTCTCCTT 
     7321 CCGTGTTTCA GTTAGCCTCC CCCTAGGGTG GGCGAAGAAC TCCAGCATGA GATCCCCGCG 
     7381 CTGGAGGATC ATCCAGCCGG CGTCCCGGAA AACGATTCCG AAGCCCAACC TTTCATAGAA 
     7441 GGCGGCGGTG GAATCGAAAT CTCGTGATGG CAGGTTGGGC GTCGCTTGGT CGGTCATTTC 
     7501 GAACCCCAGA GTCCCGCTCA GAAGAACTCG TCAAGAAGGC GATAGAAGGC GATGCGCTGC 
     7561 GAATCGGGAG CGGCGATACC GTAAAGCACG AGGAAGCGGT CAGCCCATTC GCCGCCAAGC 
     7621 TCTTCAGCAA TATCACGGGT AGCCAACGCT ATGTCCTGAT AGCGATCCGC CACACCCAGC 
     7681 CGGCCACAGT CGATGAATCC AGAAAAGCGG CCATTTTCCA CCATGATATT CGGCAAGCAG 
     7741 GCATCGCCAT GGGTCACGAC GAGATCCTCG CCGTCGGGCA TGCTCGCCTT GAGCCTGGCG 
     7801 AACAGTTCGG CTGGCGCGAG CCCCTGATGC TCTTCGTCCA GATCATCCTG ATCGACAAGA 
     7861 CCGGCTTCCA TCCGAGTACG TGCTCGCTCG ATGCGATGTT TCGCTTGGTG GTCGAATGGG 
     7921 CAGGTAGCCG GATCAAGCGT ATGCAGCCGC CGCATTGCAT CAGCCATGAT GGATACTTTC 
     7981 TCGGCAGGAG CAAGGTGAGA TGACAGGAGA TCCTGCCCCG GCACTTCGCC CAATAGCAGC 
     8041 CAGTCCCTTC CCGCTTCAGT GACAACGTCG AGCACAGCTG CGCAAGGAAC GCCCGTCGTG 
     8101 GCCAGCCACG ATAGCCGCGC TGCCTCGTCT TGCAGTTCAT TCAGGGCACC GGACAGGTCG 
     8161 GTCTTGACAA AAAGAACCGG GCGCCCCTGC GCTGACAGCC GGAACACGGC GGCATCAGAG 
     8221 CAGCCGATTG TCTGTTGTGC CCAGTCATAG CCGAATAGCC TCTCCACCCA AGCGGCCGGA 
     8281 GAACCTGCGT GCAATCCATC TTGTTCAATC ATGCGAAACG ATCCTCATCC TGTCTCTTGA 
     8341 TCGATCTTTG CAAAAGCCTA GGCCTCCAAA AAAGCCTCCT CACTACTTCT GGAATAGCTC 
     8401 AGAGGCCGAG GCGGCCTCGG CCTCTGCATA AATAAAAAAA ATTAGTCAGC CATGGGGCGG 
     8461 AGAATGGGCG GAACTGGGCG GAGTTAGGGG CGGGATGGGC GGAGTTAGGG GCGGGACTAT 
     8521 GGTTGCTGAC TAATTGAGAT GCATGCTTTG CATACTTCTG CCTGCTGGGG AGCCTGGGGA 
     8581 CTTTCCACAC CTGGTTGCTG ACTAATTGAG ATGCATGCTT TGCATACTTC TGCCTGCTGG 
     8641 GGAGCCTGGG GACTTTCCAC ACCCTAACTG ACACACATTC CACAGCTGGT TCTTTCCGCC 
     8701 TCAGGACTCT TCCTTTTTCA ATATTATTGA AGCATTTATC AGGGTTATTG TCTCATGAGC 
     8761 GGATACATAT TTGAATGTAT TTAGAAAAAT AAACAAATAG GGGTTCCGCG CACATTTCCC 
     8821 CGAAAAGTGC CACCTGACGC GCCCTGTAGC GGCGCATTAA GCGCGGCGGG TGTGGTGGTT 
     8881 ACGCGCAGCG TGACCGCTAC ACTTGCCAGC GCCCTAGCGC CCGCTCCTTT CGCTTTCTTC 
     8941 CCTTCCTTTC TCGCCACGTT CGCCGGCTTT CCCCGTCAAG CTCTAAATCG GGGGCTCCCT 
     9001 TTAGGGTTCC GATTTAGTGC TTTACGGCAC CTCGACCCCA AAAAACTTGA TTAGGGTGAT 
     9061 GGTTCACGTA GTGGGCCATC GCCCTGATAG ACGGTTTTTC GCCCTTTGAC GTTGGAGTCC 
     9121 ACGTTCTTTA ATAGTGGACT CTTGTTCCAA ACTGGAACAA CACTCAACCC TATCTCGGTC 
     9181 TATTCTTTTG ATTTATAAGG GATTTTGCCG ATTTCGGCCT ATTGGTTAAA AAATGAGCTG 
     9241 ATTTAACAAA AATTTAACGC GAATTTT
//