LOCUS       RC212708                7314 bp    DNA     circular  SYN 
DEFINITION  TMPRSS6 (Myc-DDK-tagged)-Human transmembrane protease, serine 6 (TMPRSS6)
ACCESSION   NM_153609
VERSION     NM_153609.2
SOURCE      synthetic construct 
FEATURES    Location/Qualifiers 
     misc_feature    201..924 
                     /label="CMV promoter"  
     misc_feature    839..856 
                     /label="Restriction Enzyme"  
     misc_feature    953..971 
                     /label="T7 promoter"  
     misc_feature    3471..3562 
                     /label="Myc-DDK"  
     misc_feature    3592..4177 
                     /label="PolyA signal"  
     misc_feature    3649..3668 
                     /label="Restriction Enzyme"  
     misc_feature    4453..5125 
                     /label="ColE1"  
     misc_feature    5555..6349 
                     /label="Kan/Neo"  
     misc_feature    6384..6686 
                     /label="SV40 ori"  
     misc_feature    6772..7249 
                     /label="f1 ori"  
     misc_feature    1020..1028 
                     /label="Restriction Enzyme"  
     misc_feature    3453..3459 
                     /label="Restriction Enzyme"  
     misc_feature    1020..3453 
                     /label="TMPRSS6"  
ORIGIN                      
        1 AACAAAATAT TAACGCTTAC AATTTCCATT CGCCATTCAG GCTGCGCAAC TGTTGGGAAG 
       61 GGCGATCGGT GCGGGCCTCT TCGCTATTAC GCCAGCTGGC GAAAGGGGGA TGTGCTGCAA 
      121 GGCGATTAAG TTGGGTAACG CCAGGGTTTT CCCAGTCACG ACGTTGTAAA ACGACGGCCA 
      181 GTGCCAAGCT GATCTATACA TTGAATCAAT ATTGGCAATT AGCCATATTA GTCATTGGTT 
      241 ATATAGCATA AATCAATATT GGCTATTGGC CATTGCATAC GTTGTATCTA TATCATAATA 
      301 TGTACATTTA TATTGGCTCA TGTCCAATAT GACCGCCATG TTGACATTGA TTATTGACTA 
      361 GTTATTAATA GTAATCAATT ACGGGGTCAT TAGTTCATAG CCCATATATG GAGTTCCGCG 
      421 TTACATAACT TACGGTAAAT GGCCCGCCTG GCTGACCGCC CAACGACCCC CGCCCATTGA 
      481 CGTCAATAAT GACGTATGTT CCCATAGTAA CGCCAATAGG GACTTTCCAT TGACGTCAAT 
      541 GGGTGGAGTA TTTACGGTAA ACTGCCCACT TGGCAGTACA TCAAGTGTAT CATATGCCAA 
      601 GTCCGCCCCC TATTGACGTC AATGACGGTA AATGGCCCGC CTGGCATTAT GCCCAGTACA 
      661 TGACCTTACG GGACTTTCCT ACTTGGCAGT ACATCTACGT ATTAGTCATC GCTATTACCA 
      721 TGGTGATGCG GTTTTGGCAG TACACCAATG GGCGTGGATA GCGGTTTGAC TCACGGGGAT 
      781 TTCCAAGTCT CCACCCCATT GACGTCAATG GGAGTTTGTT TTGGCACCAA AATCAACGGG 
      841 ACTTTCCAAA ATGTCGTAAT AACCCCGCCC CGTTGACGCA AATGGGCGGT AGGCGTGTAC 
      901 GGTGGGAGGT CTATATAAGC AGAGCTCGTT TAGTGAACCG TCAGAATTTT GTAATACGAC 
      961 TCACTATAGG GCGGCCGGGA ATTCGTCGAC TGGATCCGGT ACCGAGGAGA TCTGCCGCCG 
     1021 CGATCGCCAT GTTGTTACTC TTCCACTCCA AAAGGATGCC CGTGGCCGAG GCCCCCCAGG 
     1081 TGGCTGGCGG GCAGGGGGAC GGAGGTGATG GCGAGGAAGC GGAGCCAGAG GGGATGTTCA 
     1141 AGGCCTGTGA GGACTCCAAG AGAAAAGCCC GGGGCTACCT CCGCCTGGTG CCCCTGTTTG 
     1201 TGCTGCTGGC CCTGCTCGTG CTGGCTTCGG CGGGGGTGCT ACTCTGGTAT TTCCTAGGGT 
     1261 ACAAGGCGGA GGTGATGGTC AGCCAGGTGT ACTCAGGCAG TCTGCGTGTA CTCAATCGCC 
     1321 ACTTCTCCCA GGATCTTACC CGCCGGGAAT CTAGTGCCTT CCGCAGTGAA ACCGCCAAAG 
     1381 CCCAGAAGAT GCTCAAGGAG CTCATCACCA GCACCCGCCT GGGAACTTAC TACAACTCCA 
     1441 GCTCCGTCTA TTCCTTTGGG GAGGGACCCC TCACCTGCTT CTTCTGGTTC ATTCTCCAAA 
     1501 TCCCCGAGCA CCGCCGGCTG ATGCTGAGCC CCGAGGTGGT GCAGGCACTG CTGGTGGAGG 
     1561 AGCTGCTGTC CACAGTCAAC AGCTCGGCTG CCGTCCCCTA CAGGGCCGAG TACGAAGTGG 
     1621 ACCCCGAGGG CCTAGTGATC CTGGAAGCCA GTGTGAAAGA CATAGCTGCA TTGAATTCCA 
     1681 CGCTGGGTTG TTACCGCTAC AGCTACGTGG GCCAGGGCCA GGTCCTCCGG CTGAAGGGGC 
     1741 CTGACCACCT GGCCTCCAGC TGCCTGTGGC ACCTGCAGGG CCCCGAGGAC CTCATGCTCA 
     1801 AACTCCGGCT GGAGTGGACG CTGGCAGAGT GCCGGGACCG ACTGGCCATG TATGACGTGG 
     1861 CCGGGCCCCT GGAGAAGAGG CTCATCACCT CGGTGTACGG CTGCAGCCGC CAGGAGCCCG 
     1921 TGGTGGAGGT TCTGGCGTCG GGGGCCATCA TGGCGGTCGT CTGGAAGAAG GGCCTGCACA 
     1981 GCTACTACGA CCCCTTCGTG CTCTCCGTGC AGCCGGTGGT CTTCCAGGCC TGTGAAGTGA 
     2041 ACCTGACGCT GGACAACAGG CTCGACTCCC AGGGCGTCCT CAGCACCCCG TACTTCCCCA 
     2101 GCTACTACTC ACCCCAAACC CACTGCTCCT GGCACCTCAC GGTGCCCTCT CTGGACTACG 
     2161 GCTTGGCCCT CTGGTTTGAT GCCTATGCAC TGAGGAGGCA GAAGTATGAT TTGCCGTGCA 
     2221 CCCAGGGCCA GTGGACGATC CAGAACAGGA GGCTGTGTGG CTTGCGCATC CTGCAGCCCT 
     2281 ACGCCGAGAG GATCCCCGTG GTGGCCACGG CCGGGATCAC CATCAACTTC ACCTCCCAGA 
     2341 TCTCCCTCAC CGGGCCCGGT GTGCGGGTGC ACTATGGCTT GTACAACCAG TCGGACCCCT 
     2401 GCCCTGGAGA GTTCCTCTGT TCTGTGAATG GACTCTGTGT CCCTGCCTGT GATGGGGTCA 
     2461 AGGACTGCCC CAACGGCCTG GATGAGAGAA ACTGCGTTTG CAGAGCCACA TTCCAGTGCA 
     2521 AAGAGGACAG CACATGCATC TCACTGCCCA AGGTCTGTGA TGGGCAGCCT GATTGTCTCA 
     2581 ACGGCAGCGA CGAAGAGCAG TGCCAGGAAG GGGTGCCATG TGGGACATTC ACCTTCCAGT 
     2641 GTGAGGACCG GAGCTGCGTG AAGAAGCCCA ACCCGCAGTG TGATGGGCGG CCCGACTGCA 
     2701 GGGACGGCTC GGATGAGGAG CACTGTGACT GTGGCCTCCA GGGCCCCTCC AGCCGCATTG 
     2761 TTGGTGGAGC TGTGTCCTCC GAGGGTGAGT GGCCATGGCA GGCCAGCCTC CAGGTTCGGG 
     2821 GTCGACACAT CTGTGGGGGG GCCCTCATCG CTGACCGCTG GGTGATAACA GCTGCCCACT 
     2881 GCTTCCAGGA GGACAGCATG GCCTCCACGG TGCTGTGGAC CGTGTTCCTG GGCAAGGTGT 
     2941 GGCAGAACTC GCGCTGGCCT GGAGAGGTGT CCTTCAAGGT GAGCCGCCTG CTCCTGCACC 
     3001 CGTACCACGA AGAGGACAGC CATGACTACG ACGTGGCGCT GCTGCAGCTC GACCACCCGG 
     3061 TGGTGCGCTC GGCCGCCGTG CGCCCCGTCT GCCTGCCCGC GCGCTCCCAC TTCTTCGAGC 
     3121 CCGGCCTGCA CTGCTGGATT ACGGGCTGGG GCGCCTTGCG CGAGGGCGGC CCCATCAGCA 
     3181 ACGCTCTGCA GAAAGTGGAT GTGCAGTTGA TCCCACAGGA CCTGTGCAGC GAGGTCTATC 
     3241 GCTACCAGGT GACGCCACGC ATGCTGTGTG CCGGCTACCG CAAGGGCAAG AAGGATGCCT 
     3301 GTCAGGGTGA CTCAGGTGGT CCGCTGGTGT GCAAGGCACT CAGTGGCCGC TGGTTCCTGG 
     3361 CGGGGCTGGT CAGCTGGGGC CTGGGCTGTG GCCGGCCTAA CTACTTCGGC GTCTACACCC 
     3421 GCATCACAGG TGTGATCAGC TGGATCCAGC AAGTGGTGAC CACGCGTACG CGGCCGCTCG 
     3481 AGCAGAAACT CATCTCAGAA GAGGATCTGG CAGCAAATGA TATCCTGGAT TACAAGGATG 
     3541 ACGACGATAA GGTTTAAACG GCCGGCCGCG GTCATAGCTG TTTCCTGAAC AGATCCCGGG 
     3601 TGGCATCCCT GTGACCCCTC CCCAGTGCCT CTCCTGGCCC TGGAAGTTGC CACTCCAGTG 
     3661 CCCACCAGCC TTGTCCTAAT AAAATTAAGT TGCATCATTT TGTCTGACTA GGTGTCCTTC 
     3721 TATAATATTA TGGGGTGGAG GGGGGTGGTA TGGAGCAAGG GGCAAGTTGG GAAGACAACC 
     3781 TGTAGGGCCT GCGGGGTCTA TTGGGAACCA AGCTGGAGTG CAGTGGCACA ATCTTGGCTC 
     3841 ACTGCAATCT CCGCCTCCTG GGTTCAAGCG ATTCTCCTGC CTCAGCCTCC CGAGTTGTTG 
     3901 GGATTCCAGG CATGCATGAC CAGGCTCAGC TAATTTTTGT TTTTTTGGTA GAGACGGGGT 
     3961 TTCACCATAT TGGCCAGGCT GGTCTCCAAC TCCTAATCTC AGGTGATCTA CCCACCTTGG 
     4021 CCTCCCAAAT TGCTGGGATT ACAGGCGTGA ACCACTGCTC CCTTCCCTGT CCTTCTGATT 
     4081 TTAAAATAAC TATACCAGCA GGAGGACGTC CAGACACAGC ATAGGCTACC TGGCCATGCC 
     4141 CAACCGGTGG GACATTTGAG TTGCTTGCTT GGCACTGTCC TCTCATGCGT TGGGTCCACT 
     4201 CAGTAGATGC CTGTTGAATT GGGTACGCGG CCAGCGGCGA GCGGTATCAG CTCACTCAAA 
     4261 GGCGGTAATA CGGTTATCCA CAGAATCAGG GGATAACGCA GGAAAGAACA TGTGAGCAAA 
     4321 AGGCCAGCAA AAGGCCAGGA ACCGTAAAAA GGCCGCGTTG CTGGCGTTTT TCCATAGGCT 
     4381 CCGCCCCCCT GACGAGCATC ACAAAAATCG ACGCTCAAGT CAGAGGTGGC GAAACCCGAC 
     4441 AGGACTATAA AGATACCAGG CGTTTCCCCC TGGAAGCTCC CTCGTGCGCT CTCCTGTTCC 
     4501 GACCCTGCCG CTTACCGGAT ACCTGTCCGC CTTTCTCCCT TCGGGAAGCG TGGCGCTTTC 
     4561 TCATAGCTCA CGCTGTAGGT ATCTCAGTTC GGTGTAGGTC GTTCGCTCCA AGCTGGGCTG 
     4621 TGTGCACGAA CCCCCCGTTC AGCCCGACCG CTGCGCCTTA TCCGGTAACT ATCGTCTTGA 
     4681 GTCCAACCCG GTAAGACACG ACTTATCGCC ACTGGCAGCA GCCACTGGTA ACAGGATTAG 
     4741 CAGAGCGAGG TATGTAGGCG GTGCTACAGA GTTCTTGAAG TGGTGGCCTA ACTACGGCTA 
     4801 CACTAGAAGA ACAGTATTTG GTATCTGCGC TCTGCTGAAG CCAGTTACCT TCGGAAAAAG 
     4861 AGTTGGTAGC TCTTGATCCG GCAAACAAAC CACCGCTGGT AGCGGTGGTT TTTTTGTTTG 
     4921 CAAGCAGCAG ATTACGCGCA GAAAAAAAGG ATCTCAAGAA GATCCTTTGA TCTTTTCTAC 
     4981 GGGGTCTGAC GCTCAGTGGA ACGAAAACTC ACGTTAAGGG ATTTTGGTCA TGAGATTATC 
     5041 AAAAAGGATC TTCACCTAGA TCCTTTTAAA TTAAAAATGA AGTTTTAAAT CAATCTAAAG 
     5101 TATATATGAG TAACCTGAGG CTATGGCAGG GCCTGCCGCC CCGACGTTGG CTGCGAGCCC 
     5161 TGGGCCTTCA CCCGAACTTG GGGGGTGGGG TGGGGAAAAG GAAGAAACGC GGGCGTATTG 
     5221 GCCCCAATGG GGTCTCGGTG GGGTATCGAC AGAGTGCCAG CCCTGGGACC GAACCCCGCG 
     5281 TTTATGAACA AACGACCCAA CACCGTGCGT TTTATTCTGT CTTTTTATTG CCGTCATAGC 
     5341 GCGGGTTCCT TCCGGTATTG TCTCCTTCCG TGTTTCAGTT AGCCTCCCCC TAGGGTGGGC 
     5401 GAAGAACTCC AGCATGAGAT CCCCGCGCTG GAGGATCATC CAGCCGGCGT CCCGGAAAAC 
     5461 GATTCCGAAG CCCAACCTTT CATAGAAGGC GGCGGTGGAA TCGAAATCTC GTGATGGCAG 
     5521 GTTGGGCGTC GCTTGGTCGG TCATTTCGAA CCCCAGAGTC CCGCTCAGAA GAACTCGTCA 
     5581 AGAAGGCGAT AGAAGGCGAT GCGCTGCGAA TCGGGAGCGG CGATACCGTA AAGCACGAGG 
     5641 AAGCGGTCAG CCCATTCGCC GCCAAGCTCT TCAGCAATAT CACGGGTAGC CAACGCTATG 
     5701 TCCTGATAGC GATCCGCCAC ACCCAGCCGG CCACAGTCGA TGAATCCAGA AAAGCGGCCA 
     5761 TTTTCCACCA TGATATTCGG CAAGCAGGCA TCGCCATGGG TCACGACGAG ATCCTCGCCG 
     5821 TCGGGCATGC TCGCCTTGAG CCTGGCGAAC AGTTCGGCTG GCGCGAGCCC CTGATGCTCT 
     5881 TCGTCCAGAT CATCCTGATC GACAAGACCG GCTTCCATCC GAGTACGTGC TCGCTCGATG 
     5941 CGATGTTTCG CTTGGTGGTC GAATGGGCAG GTAGCCGGAT CAAGCGTATG CAGCCGCCGC 
     6001 ATTGCATCAG CCATGATGGA TACTTTCTCG GCAGGAGCAA GGTGAGATGA CAGGAGATCC 
     6061 TGCCCCGGCA CTTCGCCCAA TAGCAGCCAG TCCCTTCCCG CTTCAGTGAC AACGTCGAGC 
     6121 ACAGCTGCGC AAGGAACGCC CGTCGTGGCC AGCCACGATA GCCGCGCTGC CTCGTCTTGC 
     6181 AGTTCATTCA GGGCACCGGA CAGGTCGGTC TTGACAAAAA GAACCGGGCG CCCCTGCGCT 
     6241 GACAGCCGGA ACACGGCGGC ATCAGAGCAG CCGATTGTCT GTTGTGCCCA GTCATAGCCG 
     6301 AATAGCCTCT CCACCCAAGC GGCCGGAGAA CCTGCGTGCA ATCCATCTTG TTCAATCATG 
     6361 CGAAACGATC CTCATCCTGT CTCTTGATCG ATCTTTGCAA AAGCCTAGGC CTCCAAAAAA 
     6421 GCCTCCTCAC TACTTCTGGA ATAGCTCAGA GGCCGAGGCG GCCTCGGCCT CTGCATAAAT 
     6481 AAAAAAAATT AGTCAGCCAT GGGGCGGAGA ATGGGCGGAA CTGGGCGGAG TTAGGGGCGG 
     6541 GATGGGCGGA GTTAGGGGCG GGACTATGGT TGCTGACTAA TTGAGATGCA TGCTTTGCAT 
     6601 ACTTCTGCCT GCTGGGGAGC CTGGGGACTT TCCACACCTG GTTGCTGACT AATTGAGATG 
     6661 CATGCTTTGC ATACTTCTGC CTGCTGGGGA GCCTGGGGAC TTTCCACACC CTAACTGACA 
     6721 CACATTCCAC AGCTGGTTCT TTCCGCCTCA GGACTCTTCC TTTTTCAATA TTATTGAAGC 
     6781 ATTTATCAGG GTTATTGTCT CATGAGCGGA TACATATTTG AATGTATTTA GAAAAATAAA 
     6841 CAAATAGGGG TTCCGCGCAC ATTTCCCCGA AAAGTGCCAC CTGACGCGCC CTGTAGCGGC 
     6901 GCATTAAGCG CGGCGGGTGT GGTGGTTACG CGCAGCGTGA CCGCTACACT TGCCAGCGCC 
     6961 CTAGCGCCCG CTCCTTTCGC TTTCTTCCCT TCCTTTCTCG CCACGTTCGC CGGCTTTCCC 
     7021 CGTCAAGCTC TAAATCGGGG GCTCCCTTTA GGGTTCCGAT TTAGTGCTTT ACGGCACCTC 
     7081 GACCCCAAAA AACTTGATTA GGGTGATGGT TCACGTAGTG GGCCATCGCC CTGATAGACG 
     7141 GTTTTTCGCC CTTTGACGTT GGAGTCCACG TTCTTTAATA GTGGACTCTT GTTCCAAACT 
     7201 GGAACAACAC TCAACCCTAT CTCGGTCTAT TCTTTTGATT TATAAGGGAT TTTGCCGATT 
     7261 TCGGCCTATT GGTTAAAAAA TGAGCTGATT TAACAAAAAT TTAACGCGAA TTTT
//